Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043898.t1 | XP_003615184.1 | 98.5 | 328 | 5 | 0 | 4 | 331 | 70 | 397 | 2.20E-173 | 618.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043898.t1 | Q9QYW0 | 29.8 | 312 | 178 | 9 | 30 | 302 | 183 | 492 | 4.2e-24 | 113.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043898.t1 | G7KAI8 | 98.5 | 328 | 5 | 0 | 4 | 331 | 70 | 397 | 1.6e-173 | 618.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene065496 | MS.gene043898 | PPI |
| MS.gene75013 | MS.gene043898 | PPI |
| MS.gene043898 | MS.gene70303 | PPI |
| MS.gene043898 | MS.gene003175 | PPI |
| MS.gene053381 | MS.gene043898 | PPI |
| MS.gene043898 | MS.gene88378 | PPI |
| MS.gene043898 | MS.gene28303 | PPI |
| MS.gene003846 | MS.gene043898 | PPI |
| MS.gene062301 | MS.gene043898 | PPI |
| MS.gene063209 | MS.gene043898 | PPI |
| MS.gene063568 | MS.gene043898 | PPI |
| MS.gene01984 | MS.gene043898 | PPI |
| MS.gene043898 | MS.gene038409 | PPI |
| MS.gene043898 | MS.gene45448 | PPI |
| MS.gene043898 | MS.gene58159 | PPI |
| MS.gene65479 | MS.gene043898 | PPI |
| MS.gene28304 | MS.gene043898 | PPI |
| MS.gene043898 | MS.gene056617 | PPI |
| MS.gene048301 | MS.gene043898 | PPI |
| MS.gene031356 | MS.gene043898 | PPI |
| MS.gene02854 | MS.gene043898 | PPI |
| MS.gene030707 | MS.gene043898 | PPI |
| MS.gene28161 | MS.gene043898 | PPI |
| MS.gene029825 | MS.gene043898 | PPI |
| MS.gene017174 | MS.gene043898 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043898.t1 | MTR_5g064850 | 98.489 | 331 | 5 | 0 | 1 | 331 | 67 | 397 | 0.0 | 679 |
| MS.gene043898.t1 | MTR_5g064850 | 98.187 | 331 | 4 | 1 | 1 | 331 | 67 | 395 | 0.0 | 670 |
| MS.gene043898.t1 | MTR_7g007890 | 96.073 | 331 | 13 | 0 | 1 | 331 | 67 | 397 | 0.0 | 657 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043898.t1 | AT5G61330 | 63.492 | 315 | 110 | 1 | 16 | 330 | 138 | 447 | 1.74e-141 | 408 |
| MS.gene043898.t1 | AT5G61330 | 63.492 | 315 | 110 | 1 | 16 | 330 | 127 | 436 | 1.79e-141 | 407 |
| MS.gene043898.t1 | AT5G61330 | 63.492 | 315 | 110 | 1 | 16 | 330 | 127 | 436 | 1.79e-141 | 407 |
Find 55 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCACAGCTTGTCCTTTC+AGG | 0.086562 | 5.3:-51131619 | None:intergenic |
| TGAAAAGATAGTAAACTTTA+TGG | 0.146420 | 5.3:+51137310 | MS.gene043898:CDS |
| CTTGCAGTATGGCAACTTTC+AGG | 0.156523 | 5.3:+51134594 | MS.gene043898:intron |
| AGATCAGCTATTAAAATATT+TGG | 0.170387 | 5.3:+51134855 | MS.gene043898:CDS |
| ACTGGTGCTGCTGCAATTAA+AGG | 0.238779 | 5.3:+51134659 | MS.gene043898:CDS |
| TTTAGAAATTTCTAATGATT+TGG | 0.246736 | 5.3:+51134045 | MS.gene043898:CDS |
| GGAGACTTTGGATTCTATAT+TGG | 0.273642 | 5.3:+51132120 | MS.gene043898:CDS |
| CATCTGCTTGATCATTCTAC+TGG | 0.336534 | 5.3:-51134823 | None:intergenic |
| ACAACTTGAGAAAGAGTATA+TGG | 0.343510 | 5.3:+51129840 | MS.gene043898:CDS |
| CATGAAGAAATCACCATTAC+TGG | 0.347783 | 5.3:-51132687 | None:intergenic |
| GGACGGTGATCCTGAACTTC+TGG | 0.350227 | 5.3:+51135734 | MS.gene043898:CDS |
| AACTTCTTCCAAGGAGACTT+TGG | 0.353164 | 5.3:+51132108 | MS.gene043898:CDS |
| TCATATAAGCAGCAACTTGA+TGG | 0.354843 | 5.3:-51134794 | None:intergenic |
| ATGCTATTCGATGATCTGTT+TGG | 0.371540 | 5.3:+51137368 | MS.gene043898:CDS |
| TCAGAATCGTCCAGAAGTTC+AGG | 0.374459 | 5.3:-51135744 | None:intergenic |
| GCCATGGGAGGAAGATTTGC+CGG | 0.414884 | 5.3:-51137341 | None:intergenic |
| GAAGAACTTGAAGCATCATA+AGG | 0.425208 | 5.3:+51131586 | MS.gene043898:CDS |
| TTGCAGTATGGCAACTTTCA+GGG | 0.436730 | 5.3:+51134595 | MS.gene043898:intron |
| AGCCATTCTCAAGTTCAAAT+AGG | 0.443809 | 5.3:+51131922 | MS.gene043898:CDS |
| AAACAGAAACCACACCTTCT+GGG | 0.444736 | 5.3:-51131642 | None:intergenic |
| ATGACAATCTTGATCAAGAA+TGG | 0.451953 | 5.3:+51134068 | MS.gene043898:CDS |
| ACAATTGATCCTTCATCATC+AGG | 0.454754 | 5.3:+51135801 | MS.gene043898:CDS |
| AGATTTCTCAGATGCACAAC+AGG | 0.461402 | 5.3:+51134095 | MS.gene043898:CDS |
| AGCCTATTTGAACTTGAGAA+TGG | 0.465447 | 5.3:-51131924 | None:intergenic |
| GGAGGAAGATTTGCCGGCCG+AGG | 0.466535 | 5.3:-51137335 | None:intergenic |
| TAATGCTAGCTCTAATGAAC+CGG | 0.478598 | 5.3:+51134982 | MS.gene043898:CDS |
| TATAGAATCCAAAGTCTCCT+TGG | 0.480413 | 5.3:-51132116 | None:intergenic |
| AAACTTACAGCTCTTGCTCC+TGG | 0.488685 | 5.3:-51129873 | None:intergenic |
| ATCGAATAGCATTGGTGCCA+TGG | 0.493599 | 5.3:-51137357 | None:intergenic |
| AAACTTTATGGCTCCTCGGC+CGG | 0.497362 | 5.3:+51137322 | MS.gene043898:CDS |
| AGGATGACACAAGTGACAAC+TGG | 0.501309 | 5.3:+51134641 | MS.gene043898:CDS |
| AATTGTTTACCTGATGATGA+AGG | 0.510261 | 5.3:-51135810 | None:intergenic |
| GCCGGCAAATCTTCCTCCCA+TGG | 0.513552 | 5.3:+51137340 | MS.gene043898:CDS |
| ACCATTACTGGCTTGAGCGA+TGG | 0.525229 | 5.3:-51132675 | None:intergenic |
| GGGACAAGTCAATCAACAAA+TGG | 0.528981 | 5.3:+51134615 | MS.gene043898:CDS |
| TGACAATCTTGATCAAGAAT+GGG | 0.532967 | 5.3:+51134069 | MS.gene043898:CDS |
| TTACTGGCTTGAGCGATGGA+TGG | 0.545707 | 5.3:-51132671 | None:intergenic |
| ATCTGCTTGATCATTCTACT+GGG | 0.552705 | 5.3:-51134822 | None:intergenic |
| AACAGATCATCGAATAGCAT+TGG | 0.555701 | 5.3:-51137365 | None:intergenic |
| TCGAATAGCATTGGTGCCAT+GGG | 0.559495 | 5.3:-51137356 | None:intergenic |
| CACGGCCAACCGTGATTACG+AGG | 0.561299 | 5.3:-51137420 | None:intergenic |
| TTCTATATTGGAACTGCAAG+AGG | 0.562013 | 5.3:+51132132 | MS.gene043898:CDS |
| AGATTTGATAACTTCTTCCA+AGG | 0.564786 | 5.3:+51132099 | MS.gene043898:CDS |
| TAGTAAACTTTATGGCTCCT+CGG | 0.577305 | 5.3:+51137318 | MS.gene043898:CDS |
| TCCATCGCTCAAGCCAGTAA+TGG | 0.594131 | 5.3:+51132674 | MS.gene043898:CDS |
| GTATATGGATCTTCATCACC+AGG | 0.598970 | 5.3:+51129855 | MS.gene043898:CDS |
| ACAAGCTGTGAAAGCCCAGA+AGG | 0.601029 | 5.3:+51131628 | MS.gene043898:CDS |
| AAGGATGAAGATCTCCTGAA+AGG | 0.606031 | 5.3:+51131605 | MS.gene043898:CDS |
| AAGTTCAAATAGGCTACCCC+AGG | 0.608312 | 5.3:+51131932 | MS.gene043898:CDS |
| AATAGCATTGGTGCCATGGG+AGG | 0.612836 | 5.3:-51137353 | None:intergenic |
| CTTATGTCCCCAGGTTGCCG+AGG | 0.632820 | 5.3:+51134958 | MS.gene043898:intron |
| AGTCAATCAACAAATGGCAG+AGG | 0.633983 | 5.3:+51134621 | MS.gene043898:CDS |
| ATTAGAGCTAGCATTAACCT+CGG | 0.660903 | 5.3:-51134975 | None:intergenic |
| GCTCAGGAAACACATGCGGA+CGG | 0.674354 | 5.3:+51135717 | MS.gene043898:intron |
| TTCTGCTCAGGAAACACATG+CGG | 0.676690 | 5.3:+51135713 | MS.gene043898:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 51129835 | 51137427 | 51129835 | ID=MS.gene043898 |
| chr5.3 | mRNA | 51129835 | 51137427 | 51129835 | ID=MS.gene043898.t1;Parent=MS.gene043898 |
| chr5.3 | exon | 51129835 | 51129887 | 51129835 | ID=MS.gene043898.t1.exon1;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51129835 | 51129887 | 51129835 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51131577 | 51131649 | 51131577 | ID=MS.gene043898.t1.exon2;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51131577 | 51131649 | 51131577 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51131879 | 51131953 | 51131879 | ID=MS.gene043898.t1.exon3;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51131879 | 51131953 | 51131879 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51132043 | 51132153 | 51132043 | ID=MS.gene043898.t1.exon4;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51132043 | 51132153 | 51132043 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51132653 | 51132695 | 51132653 | ID=MS.gene043898.t1.exon5;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51132653 | 51132695 | 51132653 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51134023 | 51134116 | 51134023 | ID=MS.gene043898.t1.exon6;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51134023 | 51134116 | 51134023 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51134601 | 51134703 | 51134601 | ID=MS.gene043898.t1.exon7;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51134601 | 51134703 | 51134601 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51134786 | 51134881 | 51134786 | ID=MS.gene043898.t1.exon8;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51134786 | 51134881 | 51134786 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51134971 | 51135003 | 51134971 | ID=MS.gene043898.t1.exon9;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51134971 | 51135003 | 51134971 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51135723 | 51135822 | 51135723 | ID=MS.gene043898.t1.exon10;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51135723 | 51135822 | 51135723 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51137116 | 51137200 | 51137116 | ID=MS.gene043898.t1.exon11;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51137116 | 51137200 | 51137116 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| chr5.3 | exon | 51137298 | 51137427 | 51137298 | ID=MS.gene043898.t1.exon12;Parent=MS.gene043898.t1 |
| chr5.3 | CDS | 51137298 | 51137427 | 51137298 | ID=cds.MS.gene043898.t1;Parent=MS.gene043898.t1 |
| Gene Sequence |
| Protein sequence |
