Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05096.t1 | XP_024635738.1 | 95.1 | 123 | 6 | 0 | 5 | 127 | 58 | 180 | 8.00E-60 | 239.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05096.t1 | F4KEV7 | 64.2 | 120 | 43 | 0 | 4 | 123 | 56 | 175 | 1.0e-39 | 164.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05096.t1 | A0A396IQN5 | 95.9 | 121 | 5 | 0 | 7 | 127 | 32 | 152 | 7.6e-60 | 239.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050612 | MS.gene05096 | -0.816803 | 4.58E-52 | -1.69E-46 |
| MS.gene050675 | MS.gene05096 | -0.815191 | 1.05E-51 | -1.69E-46 |
| MS.gene05096 | MS.gene055942 | 0.805846 | 1.10E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene059865 | -0.800512 | 1.39E-48 | -1.69E-46 |
| MS.gene05096 | MS.gene061565 | 0.806427 | 8.27E-50 | -1.69E-46 |
| MS.gene05096 | MS.gene064019 | 0.808553 | 2.93E-50 | -1.69E-46 |
| MS.gene05096 | MS.gene066003 | -0.802565 | 5.28E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene072378 | 0.80726 | 5.51E-50 | -1.69E-46 |
| MS.gene05096 | MS.gene072875 | -0.804818 | 1.80E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene074568 | -0.801437 | 9.01E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene22726 | -0.804315 | 2.29E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene22826 | -0.801182 | 1.02E-48 | -1.69E-46 |
| MS.gene05096 | MS.gene24883 | 0.805032 | 1.62E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene26384 | -0.801906 | 7.22E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene26662 | -0.800891 | 1.17E-48 | -1.69E-46 |
| MS.gene05096 | MS.gene33505 | 0.80811 | 3.64E-50 | -1.69E-46 |
| MS.gene05096 | MS.gene33603 | -0.801945 | 7.09E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene35208 | 0.802051 | 6.74E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene51150 | -0.806938 | 6.45E-50 | -1.69E-46 |
| MS.gene05096 | MS.gene58012 | -0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene58060 | -0.8047 | 1.90E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene63020 | -0.812465 | 4.18E-51 | -1.69E-46 |
| MS.gene05096 | MS.gene65990 | -0.803861 | 2.85E-49 | -1.69E-46 |
| MS.gene05096 | MS.gene75048 | 0.809697 | 1.66E-50 | -1.69E-46 |
| MS.gene05096 | MS.gene82916 | -0.800695 | 1.28E-48 | -1.69E-46 |
| MS.gene05096 | MS.gene98475 | 0.800358 | 1.50E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05096.t1 | MTR_3g063030 | 88.976 | 127 | 10 | 1 | 5 | 127 | 35 | 161 | 8.87e-80 | 232 |
| MS.gene05096.t1 | MTR_3g062950 | 86.885 | 122 | 11 | 2 | 7 | 127 | 48 | 165 | 6.95e-72 | 212 |
| MS.gene05096.t1 | MTR_3g062940 | 68.908 | 119 | 37 | 0 | 9 | 127 | 78 | 196 | 2.09e-48 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05096.t1 | AT5G58240 | 65.254 | 118 | 41 | 0 | 8 | 125 | 8 | 125 | 8.40e-56 | 170 |
| MS.gene05096.t1 | AT5G58240 | 63.115 | 122 | 45 | 0 | 4 | 125 | 36 | 157 | 1.24e-55 | 171 |
| MS.gene05096.t1 | AT5G58240 | 63.115 | 122 | 45 | 0 | 4 | 125 | 56 | 177 | 1.74e-55 | 171 |
Find 16 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACGCTTCACTTCGCGCTTC+GGG | 0.265330 | 3.4:-65268432 | None:intergenic |
| CTTCTTGTGCCATTTCTTCA+AGG | 0.286997 | 3.4:-65269001 | None:intergenic |
| CAATGATGACATTTATGATT+TGG | 0.306333 | 3.4:+65268772 | MS.gene05096:CDS |
| ACACGCTTCACTTCGCGCTT+CGG | 0.356957 | 3.4:-65268433 | None:intergenic |
| TGGATTTAGAGAGGAAAGAT+AGG | 0.389495 | 3.4:+65268976 | MS.gene05096:CDS |
| AGATAGGAACCTTGAAGAAA+TGG | 0.417571 | 3.4:+65268992 | MS.gene05096:CDS |
| ATGTAAGTGATGATGCATTA+TGG | 0.441950 | 3.4:-65268531 | None:intergenic |
| AACCAGGATGGACCTCAAGC+TGG | 0.517750 | 3.4:+65268689 | MS.gene05096:intron |
| TTCATATCCATATACTTCCT+CGG | 0.540594 | 3.4:+65268729 | MS.gene05096:CDS |
| ATGATGAGAATATTGAGTTA+TGG | 0.557271 | 3.4:+65268470 | MS.gene05096:CDS |
| TGGCGGATAGCACATAAACT+TGG | 0.598736 | 3.4:+65268490 | MS.gene05096:CDS |
| GGAACTGATTGTCCAGCTTG+AGG | 0.613109 | 3.4:-65268701 | None:intergenic |
| ATGAGAATATTGAGTTATGG+CGG | 0.618988 | 3.4:+65268473 | MS.gene05096:CDS |
| GTTGAAAAGGAAGCTAGCAG+TGG | 0.629013 | 3.4:+65268956 | MS.gene05096:CDS |
| AGCTAGCAGTGGATTTAGAG+AGG | 0.630163 | 3.4:+65268967 | MS.gene05096:CDS |
| AGTATATGGATATGAACATG+AGG | 0.648556 | 3.4:-65268722 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGATGAGAATATTGAGTTA+TGG | + | chr3.4:65268470-65268489 | MS.gene05096:CDS | 25.0% |
| ! | ATGTAAACATGAATCTACTT+TGG | + | chr3.4:65268356-65268375 | MS.gene05096:intron | 25.0% |
| ! | CAATGATGACATTTATGATT+TGG | + | chr3.4:65268772-65268791 | MS.gene05096:CDS | 25.0% |
| ! | TGGAACATTTGTGTTTAATT+TGG | + | chr3.4:65268800-65268819 | MS.gene05096:intron | 25.0% |
| !!! | GTTTAATTTGGCTGTTTTTT+AGG | + | chr3.4:65268812-65268831 | MS.gene05096:intron | 25.0% |
| AAAAAGAGAAGGAGTTGAAA+AGG | + | chr3.4:65268943-65268962 | MS.gene05096:CDS | 30.0% | |
| AGTATATGGATATGAACATG+AGG | - | chr3.4:65268725-65268744 | None:intergenic | 30.0% | |
| ATGAGAATATTGAGTTATGG+CGG | + | chr3.4:65268473-65268492 | MS.gene05096:CDS | 30.0% | |
| ATTGTATATCCCTATAGCAT+TGG | - | chr3.4:65268640-65268659 | None:intergenic | 30.0% | |
| CTGGTTCAAAAAGATTGATT+TGG | - | chr3.4:65268675-65268694 | None:intergenic | 30.0% | |
| GCAGATAAATGAAAAAGAGA+AGG | + | chr3.4:65268932-65268951 | MS.gene05096:intron | 30.0% | |
| TGTATCCAAGTAAGACAATT+TGG | + | chr3.4:65268556-65268575 | MS.gene05096:intron | 30.0% | |
| TTCATATCCATATACTTCCT+CGG | + | chr3.4:65268729-65268748 | MS.gene05096:CDS | 30.0% | |
| TTTCTCCAAATTGTCTTACT+TGG | - | chr3.4:65268564-65268583 | None:intergenic | 30.0% | |
| ! | ATGTAAGTGATGATGCATTA+TGG | - | chr3.4:65268534-65268553 | None:intergenic | 30.0% |
| ! | CAAATCAATCTTTTTGAACC+AGG | + | chr3.4:65268673-65268692 | MS.gene05096:intron | 30.0% |
| ! | TGCTGTTATTTGAGCATATA+AGG | + | chr3.4:65268586-65268605 | MS.gene05096:intron | 30.0% |
| !! | ACATTTATGATTTGGTAGGA+TGG | + | chr3.4:65268780-65268799 | MS.gene05096:intron | 30.0% |
| !! | GATGACATTTATGATTTGGT+AGG | + | chr3.4:65268776-65268795 | MS.gene05096:intron | 30.0% |
| AGATAGGAACCTTGAAGAAA+TGG | + | chr3.4:65268992-65269011 | MS.gene05096:CDS | 35.0% | |
| CATATACTTCCTCGGAAAAA+CGG | + | chr3.4:65268737-65268756 | MS.gene05096:CDS | 35.0% | |
| TACCTAAGACCAATGCTATA+GGG | + | chr3.4:65268628-65268647 | MS.gene05096:intron | 35.0% | |
| TGGATTTAGAGAGGAAAGAT+AGG | + | chr3.4:65268976-65268995 | MS.gene05096:CDS | 35.0% | |
| TTACCTAAGACCAATGCTAT+AGG | + | chr3.4:65268627-65268646 | MS.gene05096:intron | 35.0% | |
| ! | TCAATCTTTTTGAACCAGGA+TGG | + | chr3.4:65268677-65268696 | MS.gene05096:intron | 35.0% |
| !!! | GGCATTTTTGAAGCTGAATT+TGG | + | chr3.4:65268377-65268396 | MS.gene05096:intron | 35.0% |
| !! | ATCCCTATAGCATTGGTCTT+AGG | - | chr3.4:65268633-65268652 | None:intergenic | 40.0% |
| !! | CGTTTTTCCGAGGAAGTATA+TGG | - | chr3.4:65268739-65268758 | None:intergenic | 40.0% |
| !! | CTTCTTGTGCCATTTCTTCA+AGG | - | chr3.4:65269004-65269023 | None:intergenic | 40.0% |
| GTTGAAAAGGAAGCTAGCAG+TGG | + | chr3.4:65268956-65268975 | MS.gene05096:CDS | 45.0% | |
| ! | AGCTAGCAGTGGATTTAGAG+AGG | + | chr3.4:65268967-65268986 | MS.gene05096:CDS | 45.0% |
| ! | TGGCGGATAGCACATAAACT+TGG | + | chr3.4:65268490-65268509 | MS.gene05096:CDS | 45.0% |
| !! | TCGAAATCACCGTTTTTCCG+AGG | - | chr3.4:65268749-65268768 | None:intergenic | 45.0% |
| GGAACTGATTGTCCAGCTTG+AGG | - | chr3.4:65268704-65268723 | None:intergenic | 50.0% | |
| AACCAGGATGGACCTCAAGC+TGG | + | chr3.4:65268689-65268708 | MS.gene05096:intron | 55.0% | |
| ACACGCTTCACTTCGCGCTT+CGG | - | chr3.4:65268436-65268455 | None:intergenic | 55.0% | |
| CACGCTTCACTTCGCGCTTC+GGG | - | chr3.4:65268435-65268454 | None:intergenic | 60.0% | |
| GTCCAGCTTGAGGTCCATCC+TGG | - | chr3.4:65268694-65268713 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 65268322 | 65269052 | 65268322 | ID=MS.gene05096 |
| chr3.4 | mRNA | 65268322 | 65269052 | 65268322 | ID=MS.gene05096.t1;Parent=MS.gene05096 |
| chr3.4 | exon | 65268322 | 65268339 | 65268322 | ID=MS.gene05096.t1.exon1;Parent=MS.gene05096.t1 |
| chr3.4 | CDS | 65268322 | 65268339 | 65268322 | ID=cds.MS.gene05096.t1;Parent=MS.gene05096.t1 |
| chr3.4 | exon | 65268415 | 65268564 | 65268415 | ID=MS.gene05096.t1.exon2;Parent=MS.gene05096.t1 |
| chr3.4 | CDS | 65268415 | 65268564 | 65268415 | ID=cds.MS.gene05096.t1;Parent=MS.gene05096.t1 |
| chr3.4 | exon | 65268695 | 65268793 | 65268695 | ID=MS.gene05096.t1.exon3;Parent=MS.gene05096.t1 |
| chr3.4 | CDS | 65268695 | 65268793 | 65268695 | ID=cds.MS.gene05096.t1;Parent=MS.gene05096.t1 |
| chr3.4 | exon | 65268936 | 65269052 | 65268936 | ID=MS.gene05096.t1.exon4;Parent=MS.gene05096.t1 |
| chr3.4 | CDS | 65268936 | 65269052 | 65268936 | ID=cds.MS.gene05096.t1;Parent=MS.gene05096.t1 |
| Gene Sequence |
| Protein sequence |
