AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072875


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072875.t1 MTR_3g106820 98.780 164 2 0 1 164 46 209 4.24e-113 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072875.t1 AT4G32260 78.431 153 31 1 11 161 66 218 6.95e-70 211

Find 35 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGAAGCTCTTGCAAATT+TGG 0.138611 3.2:+87385208 MS.gene072875:CDS
TTCCAACTCCTTCACTTCTT+CGG 0.354892 3.2:-87385054 None:intergenic
AGAAATTCAACGACTATGAT+TGG 0.371223 3.2:-87384924 None:intergenic
CACCCCTCTCGGAACCTTCA+TGG 0.382299 3.2:+87384980 MS.gene072875:CDS
GAACCTTGTTAACAATATCT+TGG 0.384952 3.2:-87385286 None:intergenic
TTGAGATATCAACGAGAAAC+AGG 0.396683 3.2:-87385311 None:intergenic
TGCAAATTTGGAAAAGCAAA+AGG 0.407369 3.2:+87385220 MS.gene072875:CDS
CGCAGCTCTCAAAACTGCAT+TGG 0.476740 3.2:-87385084 None:intergenic
GAAATTCAACGACTATGATT+GGG 0.478683 3.2:-87384923 None:intergenic
CGCGTCACGATTGTCCATGA+AGG 0.481050 3.2:-87384994 None:intergenic
CTTCACTTCTTCGGATGTGT+TGG 0.483273 3.2:-87385045 None:intergenic
AAACTCTACTTCACCCCTCT+CGG 0.522559 3.2:+87384969 MS.gene072875:CDS
AGAGAGGGAAGTTGCTGCTA+GGG 0.528221 3.2:-87384835 None:intergenic
AAGAGAGGGAAGTTGCTGCT+AGG 0.534616 3.2:-87384836 None:intergenic
GGAAGTTGCTGCTAGGGTCA+TGG 0.539237 3.2:-87384829 None:intergenic
TAGCCAAGATATTGTTAACA+AGG 0.566254 3.2:+87385283 MS.gene072875:CDS
AGCGAGGGAAGGTGGTGCGA+AGG 0.566516 3.2:-87384862 None:intergenic
ATCCGAAGAAGTGAAGGAGT+TGG 0.580997 3.2:+87385052 MS.gene072875:CDS
TGCGCGTGCTGAAATTGCGG+TGG 0.591536 3.2:+87385103 MS.gene072875:CDS
AAGCGAAGATTGCGGAAGGT+AGG 0.592242 3.2:+87385165 MS.gene072875:CDS
TGGTGCGAAGGCGAAAGAGA+GGG 0.595311 3.2:-87384850 None:intergenic
CGACTATGATTGGGAGAGTG+AGG 0.595912 3.2:-87384914 None:intergenic
CAACACATCCGAAGAAGTGA+AGG 0.596455 3.2:+87385046 MS.gene072875:CDS
AGCTGCGCGTGCTGAAATTG+CGG 0.598150 3.2:+87385100 MS.gene072875:CDS
GTTTGTCGAGAGCGAACATG+AGG 0.598866 3.2:-87384950 None:intergenic
GTGGTGCGAAGGCGAAAGAG+AGG 0.606516 3.2:-87384851 None:intergenic
GTCCATGAAGGTTCCGAGAG+GGG 0.611279 3.2:-87384982 None:intergenic
TGTCCATGAAGGTTCCGAGA+GGG 0.615991 3.2:-87384983 None:intergenic
AATCGTGACGCGGAAATCAG+AGG 0.616507 3.2:+87385005 MS.gene072875:CDS
GATTGCGGAAGGTAGGAAGA+AGG 0.621119 3.2:+87385172 MS.gene072875:CDS
GTTGAAGCGAAGATTGCGGA+AGG 0.664257 3.2:+87385161 MS.gene072875:CDS
GAGGTTGAAATCGAAGAGTG+CGG 0.686862 3.2:-87384895 None:intergenic
TGAAGTTGAAGCGAAGATTG+CGG 0.692066 3.2:+87385157 MS.gene072875:CDS
CTTCATGGACAATCGTGACG+CGG 0.701097 3.2:+87384995 MS.gene072875:CDS
TTGTCCATGAAGGTTCCGAG+AGG 0.735316 3.2:-87384984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAATTCAACGACTATGAT+TGG - chr3.2:87384927-87384946 None:intergenic 30.0%
GAAATTCAACGACTATGATT+GGG - chr3.2:87384926-87384945 None:intergenic 30.0%
GAACCTTGTTAACAATATCT+TGG - chr3.2:87385289-87385308 None:intergenic 30.0%
TAGCCAAGATATTGTTAACA+AGG + chr3.2:87385283-87385302 MS.gene072875:CDS 30.0%
TGCAAATTTGGAAAAGCAAA+AGG + chr3.2:87385220-87385239 MS.gene072875:CDS 30.0%
GCAAGAAGCTCTTGCAAATT+TGG + chr3.2:87385208-87385227 MS.gene072875:CDS 40.0%
TGAAGTTGAAGCGAAGATTG+CGG + chr3.2:87385157-87385176 MS.gene072875:CDS 40.0%
TTCCAACTCCTTCACTTCTT+CGG - chr3.2:87385057-87385076 None:intergenic 40.0%
AAACTCTACTTCACCCCTCT+CGG + chr3.2:87384969-87384988 MS.gene072875:CDS 45.0%
ATCCGAAGAAGTGAAGGAGT+TGG + chr3.2:87385052-87385071 MS.gene072875:CDS 45.0%
CAACACATCCGAAGAAGTGA+AGG + chr3.2:87385046-87385065 MS.gene072875:CDS 45.0%
CTTCACTTCTTCGGATGTGT+TGG - chr3.2:87385048-87385067 None:intergenic 45.0%
GAGGTTGAAATCGAAGAGTG+CGG - chr3.2:87384898-87384917 None:intergenic 45.0%
AAGAGAGGGAAGTTGCTGCT+AGG - chr3.2:87384839-87384858 None:intergenic 50.0%
AAGCGAAGATTGCGGAAGGT+AGG + chr3.2:87385165-87385184 MS.gene072875:CDS 50.0%
AATCGTGACGCGGAAATCAG+AGG + chr3.2:87385005-87385024 MS.gene072875:CDS 50.0%
AGAGAGGGAAGTTGCTGCTA+GGG - chr3.2:87384838-87384857 None:intergenic 50.0%
CGACTATGATTGGGAGAGTG+AGG - chr3.2:87384917-87384936 None:intergenic 50.0%
CGCAGCTCTCAAAACTGCAT+TGG - chr3.2:87385087-87385106 None:intergenic 50.0%
CTTCATGGACAATCGTGACG+CGG + chr3.2:87384995-87385014 MS.gene072875:CDS 50.0%
GATTGCGGAAGGTAGGAAGA+AGG + chr3.2:87385172-87385191 MS.gene072875:CDS 50.0%
GTTGAAGCGAAGATTGCGGA+AGG + chr3.2:87385161-87385180 MS.gene072875:CDS 50.0%
GTTTGTCGAGAGCGAACATG+AGG - chr3.2:87384953-87384972 None:intergenic 50.0%
TGTCCATGAAGGTTCCGAGA+GGG - chr3.2:87384986-87385005 None:intergenic 50.0%
TTGTCCATGAAGGTTCCGAG+AGG - chr3.2:87384987-87385006 None:intergenic 50.0%
! TTTTCGAAAGCGAGGGAAGG+TGG - chr3.2:87384873-87384892 None:intergenic 50.0%
!!! GCTTTTTCGAAAGCGAGGGA+AGG - chr3.2:87384876-87384895 None:intergenic 50.0%
!!! TGCGGCTTTTTCGAAAGCGA+GGG - chr3.2:87384880-87384899 None:intergenic 50.0%
AGCTGCGCGTGCTGAAATTG+CGG + chr3.2:87385100-87385119 MS.gene072875:CDS 55.0%
CGCGTCACGATTGTCCATGA+AGG - chr3.2:87384997-87385016 None:intergenic 55.0%
GTCCATGAAGGTTCCGAGAG+GGG - chr3.2:87384985-87385004 None:intergenic 55.0%
TGGTGCGAAGGCGAAAGAGA+GGG - chr3.2:87384853-87384872 None:intergenic 55.0%
!!! GTGCGGCTTTTTCGAAAGCG+AGG - chr3.2:87384881-87384900 None:intergenic 55.0%
CACCCCTCTCGGAACCTTCA+TGG + chr3.2:87384980-87384999 MS.gene072875:CDS 60.0%
GTGGTGCGAAGGCGAAAGAG+AGG - chr3.2:87384854-87384873 None:intergenic 60.0%
TGCGCGTGCTGAAATTGCGG+TGG + chr3.2:87385103-87385122 MS.gene072875:CDS 60.0%
AGCGAGGGAAGGTGGTGCGA+AGG - chr3.2:87384865-87384884 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 87384831 87385325 87384831 ID=MS.gene072875
chr3.2 mRNA 87384831 87385325 87384831 ID=MS.gene072875.t1;Parent=MS.gene072875
chr3.2 exon 87384831 87385325 87384831 ID=MS.gene072875.t1.exon1;Parent=MS.gene072875.t1
chr3.2 CDS 87384831 87385325 87384831 ID=cds.MS.gene072875.t1;Parent=MS.gene072875.t1
Gene Sequence

>MS.gene072875

ATGACCCTAGCAGCAACTTCCCTCTCTTTCGCCTTCGCACCACCTTCCCTCGCTTTCGAAAAAGCCGCACTCTTCGATTTCAACCTCACTCTCCCAATCATAGTCGTTGAATTTCTCTTCCTCATGTTCGCTCTCGACAAACTCTACTTCACCCCTCTCGGAACCTTCATGGACAATCGTGACGCGGAAATCAGAGGCAAACTCAACAGCGTCACCAACACATCCGAAGAAGTGAAGGAGTTGGAAGAACAAGCCAATGCAGTTTTGAGAGCTGCGCGTGCTGAAATTGCGGTGGCGCTGAATCAGATGAAGAAAGAGACTCAAGCTGAAGTTGAAGCGAAGATTGCGGAAGGTAGGAAGAAGGTTGATGCTGAATTGCAAGAAGCTCTTGCAAATTTGGAAAAGCAAAAGGAAGAAACTGTTAAAGCTCTTGATTCTCAGATTGCTGCTCTTAGCCAAGATATTGTTAACAAGGTTCTTCCTGTTTCTCGTTGA

Protein sequence

>MS.gene072875.t1

MTLAATSLSFAFAPPSLAFEKAALFDFNLTLPIIVVEFLFLMFALDKLYFTPLGTFMDNRDAEIRGKLNSVTNTSEEVKELEEQANAVLRAARAEIAVALNQMKKETQAEVEAKIAEGRKKVDAELQEALANLEKQKEETVKALDSQIAALSQDIVNKVLPVSR