Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33505.t1 | XP_003602163.1 | 97.3 | 222 | 6 | 0 | 1 | 222 | 1 | 222 | 2.40E-115 | 424.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33505.t1 | Q9LU33 | 51.8 | 226 | 103 | 2 | 1 | 221 | 8 | 232 | 1.9e-57 | 223.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33505.t1 | G7J670 | 97.3 | 222 | 6 | 0 | 1 | 222 | 1 | 222 | 1.7e-115 | 424.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene33505 | -0.838157 | 3.40E-57 | -1.69E-46 |
| MS.gene049217 | MS.gene33505 | -0.812519 | 4.07E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene33505 | -0.829123 | 6.14E-55 | -1.69E-46 |
| MS.gene049232 | MS.gene33505 | -0.847472 | 1.13E-59 | -1.69E-46 |
| MS.gene049259 | MS.gene33505 | 0.842435 | 2.59E-58 | -1.69E-46 |
| MS.gene049285 | MS.gene33505 | -0.829564 | 4.80E-55 | -1.69E-46 |
| MS.gene049320 | MS.gene33505 | -0.838969 | 2.10E-57 | -1.69E-46 |
| MS.gene049611 | MS.gene33505 | 0.851215 | 1.03E-60 | -1.69E-46 |
| MS.gene049633 | MS.gene33505 | -0.830422 | 2.96E-55 | -1.69E-46 |
| MS.gene049634 | MS.gene33505 | 0.813322 | 2.71E-51 | -1.69E-46 |
| MS.gene050082 | MS.gene33505 | 0.861908 | 7.37E-64 | -1.69E-46 |
| MS.gene05010 | MS.gene33505 | -0.82878 | 7.43E-55 | -1.69E-46 |
| MS.gene050163 | MS.gene33505 | -0.820667 | 6.08E-53 | -1.69E-46 |
| MS.gene050165 | MS.gene33505 | -0.841683 | 4.10E-58 | -1.69E-46 |
| MS.gene050170 | MS.gene33505 | -0.857893 | 1.20E-62 | -1.69E-46 |
| MS.gene050216 | MS.gene33505 | -0.829325 | 5.48E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene33505 | -0.844621 | 6.75E-59 | -1.69E-46 |
| MS.gene050333 | MS.gene33505 | -0.828789 | 7.39E-55 | -1.69E-46 |
| MS.gene050338 | MS.gene33505 | 0.813374 | 2.64E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene33505 | 0.839557 | 1.48E-57 | -1.69E-46 |
| MS.gene050444 | MS.gene33505 | -0.804752 | 1.86E-49 | -1.69E-46 |
| MS.gene050496 | MS.gene33505 | -0.858306 | 9.02E-63 | -1.69E-46 |
| MS.gene050504 | MS.gene33505 | -0.869683 | 2.58E-66 | -1.69E-46 |
| MS.gene050518 | MS.gene33505 | -0.845844 | 3.15E-59 | -1.69E-46 |
| MS.gene050577 | MS.gene33505 | -0.8412 | 5.49E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene33505 | -0.87991 | 8.47E-70 | -1.69E-46 |
| MS.gene050647 | MS.gene33505 | 0.814243 | 1.70E-51 | -1.69E-46 |
| MS.gene050662 | MS.gene33505 | -0.807274 | 5.48E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene33505 | -0.855707 | 5.27E-62 | -1.69E-46 |
| MS.gene050699 | MS.gene33505 | -0.823412 | 1.40E-53 | -1.69E-46 |
| MS.gene050772 | MS.gene33505 | -0.810911 | 9.11E-51 | -1.69E-46 |
| MS.gene05079 | MS.gene33505 | 0.806727 | 7.15E-50 | -1.69E-46 |
| MS.gene05080 | MS.gene33505 | -0.811447 | 6.97E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene33505 | -0.843243 | 1.58E-58 | -1.69E-46 |
| MS.gene050871 | MS.gene33505 | -0.842633 | 2.30E-58 | -1.69E-46 |
| MS.gene050876 | MS.gene33505 | -0.830653 | 2.60E-55 | -1.69E-46 |
| MS.gene050883 | MS.gene33505 | 0.822248 | 2.62E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene33505 | -0.846892 | 1.63E-59 | -1.69E-46 |
| MS.gene05096 | MS.gene33505 | 0.80811 | 3.64E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene33505 | -0.844821 | 5.96E-59 | -1.69E-46 |
| MS.gene05104 | MS.gene33505 | 0.823721 | 1.19E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene33505 | 0.824161 | 9.38E-54 | -1.69E-46 |
| MS.gene051138 | MS.gene33505 | 0.849453 | 3.20E-60 | -1.69E-46 |
| MS.gene051142 | MS.gene33505 | 0.805406 | 1.36E-49 | -1.69E-46 |
| MS.gene051268 | MS.gene33505 | -0.854076 | 1.57E-61 | -1.69E-46 |
| MS.gene051317 | MS.gene33505 | -0.849586 | 2.94E-60 | -1.69E-46 |
| MS.gene051364 | MS.gene33505 | 0.831797 | 1.36E-55 | -1.69E-46 |
| MS.gene051366 | MS.gene33505 | 0.833818 | 4.29E-56 | -1.69E-46 |
| MS.gene051367 | MS.gene33505 | 0.823209 | 1.57E-53 | -1.69E-46 |
| MS.gene051422 | MS.gene33505 | 0.842622 | 2.31E-58 | -1.69E-46 |
| MS.gene051651 | MS.gene33505 | 0.837892 | 3.98E-57 | -1.69E-46 |
| MS.gene051863 | MS.gene33505 | 0.80753 | 4.83E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene33505 | -0.822209 | 2.68E-53 | -1.69E-46 |
| MS.gene052482 | MS.gene33505 | -0.840571 | 8.02E-58 | -1.69E-46 |
| MS.gene052486 | MS.gene33505 | -0.841349 | 5.02E-58 | -1.69E-46 |
| MS.gene052506 | MS.gene33505 | -0.803019 | 4.26E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene33505 | 0.82287 | 1.88E-53 | -1.69E-46 |
| MS.gene052822 | MS.gene33505 | -0.813567 | 2.40E-51 | -1.69E-46 |
| MS.gene052966 | MS.gene33505 | -0.821014 | 5.06E-53 | -1.69E-46 |
| MS.gene053203 | MS.gene33505 | -0.815092 | 1.10E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene33505 | 0.867803 | 1.05E-65 | -1.69E-46 |
| MS.gene053479 | MS.gene33505 | -0.818949 | 1.50E-52 | -1.69E-46 |
| MS.gene054056 | MS.gene33505 | 0.828338 | 9.51E-55 | -1.69E-46 |
| MS.gene054595 | MS.gene33505 | -0.823783 | 1.15E-53 | -1.69E-46 |
| MS.gene054642 | MS.gene33505 | -0.81228 | 4.59E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene33505 | 0.825364 | 4.88E-54 | -1.69E-46 |
| MS.gene054708 | MS.gene33505 | 0.804374 | 2.23E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene33505 | -0.841453 | 4.71E-58 | -1.69E-46 |
| MS.gene054954 | MS.gene33505 | -0.852486 | 4.47E-61 | -1.69E-46 |
| MS.gene055154 | MS.gene33505 | 0.842691 | 2.21E-58 | -1.69E-46 |
| MS.gene055158 | MS.gene33505 | 0.817926 | 2.56E-52 | -1.69E-46 |
| MS.gene055202 | MS.gene33505 | 0.829219 | 5.82E-55 | -1.69E-46 |
| MS.gene055362 | MS.gene33505 | -0.826973 | 2.02E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene33505 | -0.841983 | 3.41E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene33505 | -0.834042 | 3.77E-56 | -1.69E-46 |
| MS.gene055748 | MS.gene33505 | -0.847956 | 8.33E-60 | -1.69E-46 |
| MS.gene055750 | MS.gene33505 | -0.841922 | 3.54E-58 | -1.69E-46 |
| MS.gene055751 | MS.gene33505 | -0.86234 | 5.43E-64 | -1.69E-46 |
| MS.gene055776 | MS.gene33505 | -0.838279 | 3.16E-57 | -1.69E-46 |
| MS.gene05589 | MS.gene33505 | -0.876621 | 1.21E-68 | -1.69E-46 |
| MS.gene055942 | MS.gene33505 | 0.821914 | 3.13E-53 | -1.69E-46 |
| MS.gene056046 | MS.gene33505 | -0.804185 | 2.44E-49 | -1.69E-46 |
| MS.gene056224 | MS.gene33505 | 0.804901 | 1.73E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene33505 | -0.817527 | 3.15E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene33505 | -0.880001 | 7.86E-70 | -1.69E-46 |
| MS.gene056510 | MS.gene33505 | -0.820939 | 5.26E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene33505 | -0.831975 | 1.23E-55 | -1.69E-46 |
| MS.gene056692 | MS.gene33505 | -0.837002 | 6.72E-57 | -1.69E-46 |
| MS.gene056856 | MS.gene33505 | 0.851878 | 6.66E-61 | -1.69E-46 |
| MS.gene056857 | MS.gene33505 | 0.816008 | 6.90E-52 | -1.69E-46 |
| MS.gene057473 | MS.gene33505 | 0.802759 | 4.82E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene33505 | 0.830774 | 2.43E-55 | -1.69E-46 |
| MS.gene057831 | MS.gene33505 | -0.81278 | 3.57E-51 | -1.69E-46 |
| MS.gene058068 | MS.gene33505 | 0.809275 | 2.05E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene33505 | 0.846001 | 2.86E-59 | -1.69E-46 |
| MS.gene058109 | MS.gene33505 | 0.835055 | 2.10E-56 | -1.69E-46 |
| MS.gene058111 | MS.gene33505 | 0.846603 | 1.96E-59 | -1.69E-46 |
| MS.gene058117 | MS.gene33505 | 0.816127 | 6.49E-52 | -1.69E-46 |
| MS.gene058171 | MS.gene33505 | 0.847221 | 1.33E-59 | -1.69E-46 |
| MS.gene058173 | MS.gene33505 | 0.837501 | 5.01E-57 | -1.69E-46 |
| MS.gene058175 | MS.gene33505 | 0.848413 | 6.23E-60 | -1.69E-46 |
| MS.gene058268 | MS.gene33505 | 0.844743 | 6.26E-59 | -1.69E-46 |
| MS.gene058269 | MS.gene33505 | 0.827279 | 1.71E-54 | -1.69E-46 |
| MS.gene058334 | MS.gene33505 | 0.834831 | 2.39E-56 | -1.69E-46 |
| MS.gene058335 | MS.gene33505 | -0.806022 | 1.01E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene33505 | -0.813991 | 1.93E-51 | -1.69E-46 |
| MS.gene058382 | MS.gene33505 | 0.800099 | 1.69E-48 | -1.69E-46 |
| MS.gene058419 | MS.gene33505 | -0.834749 | 2.50E-56 | -1.69E-46 |
| MS.gene058493 | MS.gene33505 | 0.810335 | 1.21E-50 | -1.69E-46 |
| MS.gene058773 | MS.gene33505 | -0.825958 | 3.53E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene33505 | -0.810467 | 1.14E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene33505 | -0.852532 | 4.34E-61 | -1.69E-46 |
| MS.gene059023 | MS.gene33505 | -0.816834 | 4.51E-52 | -1.69E-46 |
| MS.gene059045 | MS.gene33505 | 0.818375 | 2.03E-52 | -1.69E-46 |
| MS.gene059070 | MS.gene33505 | 0.800003 | 1.77E-48 | -1.69E-46 |
| MS.gene059079 | MS.gene33505 | -0.801114 | 1.05E-48 | -1.69E-46 |
| MS.gene059367 | MS.gene33505 | -0.805763 | 1.14E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene33505 | -0.84413 | 9.15E-59 | -1.69E-46 |
| MS.gene059699 | MS.gene33505 | 0.800212 | 1.60E-48 | -1.69E-46 |
| MS.gene059703 | MS.gene33505 | 0.808267 | 3.37E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene33505 | -0.814016 | 1.91E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene33505 | -0.877055 | 8.54E-69 | -1.69E-46 |
| MS.gene06000 | MS.gene33505 | -0.847761 | 9.43E-60 | -1.69E-46 |
| MS.gene060405 | MS.gene33505 | 0.809499 | 1.84E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene33505 | 0.863341 | 2.67E-64 | -1.69E-46 |
| MS.gene060703 | MS.gene33505 | -0.839188 | 1.84E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene33505 | -0.858158 | 9.98E-63 | -1.69E-46 |
| MS.gene060778 | MS.gene33505 | -0.853752 | 1.94E-61 | -1.69E-46 |
| MS.gene06077 | MS.gene33505 | -0.850216 | 1.96E-60 | -1.69E-46 |
| MS.gene060823 | MS.gene33505 | 0.878574 | 2.52E-69 | -1.69E-46 |
| MS.gene06084 | MS.gene33505 | 0.809 | 2.35E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene33505 | -0.885364 | 8.66E-72 | -1.69E-46 |
| MS.gene061044 | MS.gene33505 | 0.837786 | 4.23E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33505.t1 | MTR_3g090530 | 97.297 | 222 | 6 | 0 | 1 | 222 | 1 | 222 | 8.42e-162 | 446 |
| MS.gene33505.t1 | MTR_3g090530 | 97.156 | 211 | 6 | 0 | 1 | 211 | 1 | 211 | 5.83e-153 | 423 |
| MS.gene33505.t1 | MTR_3g090530 | 98.171 | 164 | 3 | 0 | 1 | 164 | 1 | 164 | 5.61e-118 | 333 |
| MS.gene33505.t1 | MTR_1g028960 | 69.406 | 219 | 65 | 2 | 5 | 222 | 6 | 223 | 1.60e-110 | 316 |
| MS.gene33505.t1 | MTR_1g028970 | 67.411 | 224 | 70 | 2 | 1 | 222 | 1 | 223 | 4.87e-109 | 312 |
| MS.gene33505.t1 | MTR_3g090530 | 95.364 | 151 | 7 | 0 | 72 | 222 | 7 | 157 | 1.57e-105 | 301 |
| MS.gene33505.t1 | MTR_1g028970 | 68.545 | 213 | 64 | 2 | 1 | 211 | 1 | 212 | 2.79e-104 | 300 |
| MS.gene33505.t1 | MTR_3g090530 | 95.000 | 140 | 7 | 0 | 72 | 211 | 7 | 146 | 7.67e-97 | 279 |
| MS.gene33505.t1 | MTR_1g028970 | 65.882 | 170 | 55 | 2 | 1 | 168 | 1 | 169 | 4.62e-77 | 230 |
| MS.gene33505.t1 | MTR_3g090530 | 95.699 | 93 | 4 | 0 | 72 | 164 | 7 | 99 | 1.09e-61 | 188 |
| MS.gene33505.t1 | MTR_1g075710 | 35.829 | 187 | 115 | 2 | 36 | 222 | 21 | 202 | 4.22e-38 | 131 |
| MS.gene33505.t1 | MTR_1g041495 | 36.898 | 187 | 113 | 3 | 36 | 222 | 21 | 202 | 8.06e-36 | 125 |
| MS.gene33505.t1 | MTR_1g041495 | 35.204 | 196 | 113 | 4 | 36 | 222 | 21 | 211 | 1.03e-32 | 117 |
| MS.gene33505.t1 | MTR_1g075700 | 36.806 | 144 | 86 | 2 | 36 | 179 | 21 | 159 | 2.93e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33505.t1 | AT1G21360 | 51.786 | 224 | 103 | 3 | 1 | 221 | 1 | 222 | 3.66e-77 | 231 |
| MS.gene33505.t1 | AT3G21260 | 51.770 | 226 | 103 | 2 | 1 | 221 | 8 | 232 | 2.17e-70 | 215 |
| MS.gene33505.t1 | AT1G21360 | 53.293 | 167 | 73 | 3 | 1 | 164 | 1 | 165 | 4.39e-57 | 179 |
| MS.gene33505.t1 | AT1G21360 | 51.553 | 161 | 77 | 1 | 61 | 221 | 1 | 160 | 1.28e-52 | 167 |
| MS.gene33505.t1 | AT3G21260 | 52.027 | 148 | 70 | 1 | 74 | 221 | 2 | 148 | 6.50e-44 | 144 |
| MS.gene33505.t1 | AT3G21260 | 52.113 | 142 | 67 | 1 | 80 | 221 | 3 | 143 | 6.41e-42 | 139 |
| MS.gene33505.t1 | AT2G33470 | 37.158 | 183 | 110 | 2 | 40 | 222 | 25 | 202 | 1.08e-34 | 122 |
| MS.gene33505.t1 | AT2G33470 | 37.158 | 183 | 110 | 2 | 40 | 222 | 25 | 202 | 1.08e-34 | 122 |
Find 37 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCTTAACAAATTCAAATT+TGG | 0.154699 | 3.4:-82642875 | MS.gene33505:CDS |
| ATTTGGTTACCTTAATATTC+TGG | 0.236559 | 3.4:+82643155 | None:intergenic |
| CCAGCCTCATGATCTTCTTC+AGG | 0.264906 | 3.4:+82643374 | None:intergenic |
| AACCAAATTTGAATTTGTTA+AGG | 0.324351 | 3.4:+82642873 | None:intergenic |
| ACCAAATTTGAATTTGTTAA+GGG | 0.329032 | 3.4:+82642874 | None:intergenic |
| TACATGGTTCTACAAGTTCT+TGG | 0.339211 | 3.4:-82643314 | MS.gene33505:intron |
| AGACATTCACCAGAATATTA+AGG | 0.371673 | 3.4:-82643164 | MS.gene33505:intron |
| AAAGATAGGAAAGGCTTTGT+TGG | 0.395007 | 3.4:+82643341 | None:intergenic |
| GCGGCAGTAAATCCTTTGTT+TGG | 0.397158 | 3.4:-82642801 | MS.gene33505:CDS |
| GAACCATGTAACATAAAGAT+AGG | 0.421906 | 3.4:+82643327 | None:intergenic |
| CTTTGAAACCATGGCATGGA+TGG | 0.431560 | 3.4:-82642548 | MS.gene33505:CDS |
| CGAAGACTGTGACACCCTAA+TGG | 0.438068 | 3.4:-82642082 | MS.gene33505:CDS |
| AAGATAGGAAAGGCTTTGTT+GGG | 0.447613 | 3.4:+82643342 | None:intergenic |
| TTTCCTATCTTTATGTTACA+TGG | 0.447670 | 3.4:-82643330 | MS.gene33505:CDS |
| TGTCTCAAAACAGCCATTGT+TGG | 0.448554 | 3.4:+82643187 | None:intergenic |
| AATCCTTTGTTTGGCTCACT+AGG | 0.459588 | 3.4:-82642792 | MS.gene33505:intron |
| AGAAAGTGTATAACTTGGAC+AGG | 0.511125 | 3.4:-82641900 | MS.gene33505:CDS |
| TTTGCAGAAAGTGTATAACT+TGG | 0.513854 | 3.4:-82641905 | MS.gene33505:intron |
| CAATGGATATCCTCAAGAAA+AGG | 0.520025 | 3.4:+82642027 | None:intergenic |
| AAAGTATAAGGAAAATGAAG+AGG | 0.529810 | 3.4:-82643469 | None:intergenic |
| AAGCACTGAAAGCTCTTCAA+TGG | 0.530059 | 3.4:+82643409 | None:intergenic |
| GATTGTCTTGCAGATAAGGT+AGG | 0.536852 | 3.4:-82643211 | MS.gene33505:intron |
| ACAATGCTGATGTGAAATCC+AGG | 0.552433 | 3.4:+82642640 | None:intergenic |
| TGTGCCACTATGTAGATCCC+TGG | 0.554714 | 3.4:-82642658 | MS.gene33505:intron |
| ATTGTCTTGCAGATAAGGTA+GGG | 0.565172 | 3.4:-82643210 | MS.gene33505:intron |
| CCTGAAGAAGATCATGAGGC+TGG | 0.568689 | 3.4:-82643374 | MS.gene33505:CDS |
| ATGATGCAACTTTGAAACCA+TGG | 0.590512 | 3.4:-82642557 | MS.gene33505:CDS |
| AATGCAAAGAAGAGAGTTAG+CGG | 0.596549 | 3.4:-82642820 | MS.gene33505:CDS |
| GATGCAATCCATCCATGCCA+TGG | 0.623186 | 3.4:+82642540 | None:intergenic |
| CAATGCTGATGTGAAATCCA+GGG | 0.628144 | 3.4:+82642641 | None:intergenic |
| TTCAAGTCAGAAACTAGCAA+AGG | 0.631092 | 3.4:-82642844 | MS.gene33505:CDS |
| ATGTAACATAAAGATAGGAA+AGG | 0.631348 | 3.4:+82643332 | None:intergenic |
| TCACCTAGTGAGCCAAACAA+AGG | 0.631414 | 3.4:+82642789 | None:intergenic |
| TCAAGCTTACTAGAATACAA+TGG | 0.631531 | 3.4:+82642010 | None:intergenic |
| GCAACTTTGAAACCATGGCA+TGG | 0.637985 | 3.4:-82642552 | MS.gene33505:CDS |
| CAAACCTGAAGAAGATCATG+AGG | 0.654696 | 3.4:-82643378 | MS.gene33505:CDS |
| AGATAAGGTAGGGCCAACAA+TGG | 0.678454 | 3.4:-82643200 | MS.gene33505:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAATAAATATTCATC+AGG | + | chr3.4:82643418-82643437 | None:intergenic | 10.0% |
| !! | AAAAAAAAAAAAGGAAGAAA+TGG | + | chr3.4:82642849-82642868 | None:intergenic | 15.0% |
| !!! | AAACATACTTTAAATTTGTT+TGG | + | chr3.4:82642099-82642118 | None:intergenic | 15.0% |
| !!! | TTTTCAAGAAAAATAACTTA+TGG | - | chr3.4:82642594-82642613 | MS.gene33505:CDS | 15.0% |
| !! | AAAAGATCCTAAAAAGAATA+TGG | - | chr3.4:82642739-82642758 | MS.gene33505:intron | 20.0% |
| !! | CTAAACTCAAAATAATAGAA+AGG | - | chr3.4:82643025-82643044 | MS.gene33505:intron | 20.0% |
| !! | GAAAAGTGGAAAAAAAAAAA+AGG | + | chr3.4:82642858-82642877 | None:intergenic | 20.0% |
| !!! | AACCAAATTTGAATTTGTTA+AGG | + | chr3.4:82642470-82642489 | None:intergenic | 20.0% |
| !!! | ACCAAATTTGAATTTGTTAA+GGG | + | chr3.4:82642469-82642488 | None:intergenic | 20.0% |
| !!! | CTTTCTATTATTTTGAGTTT+AGG | + | chr3.4:82643027-82643046 | None:intergenic | 20.0% |
| ! | ACATATAGTTAGTTACACAT+TGG | - | chr3.4:82642353-82642372 | MS.gene33505:intron | 25.0% |
| ! | ATGTAACATAAAGATAGGAA+AGG | + | chr3.4:82642011-82642030 | None:intergenic | 25.0% |
| ! | ATTTGGTTACCTTAATATTC+TGG | + | chr3.4:82642188-82642207 | None:intergenic | 25.0% |
| ! | TCAAATTATTACATGTGTGA+AGG | + | chr3.4:82642238-82642257 | None:intergenic | 25.0% |
| ! | TCCCTTAACAAATTCAAATT+TGG | - | chr3.4:82642465-82642484 | MS.gene33505:intron | 25.0% |
| ! | TTTCCTATCTTTATGTTACA+TGG | - | chr3.4:82642010-82642029 | MS.gene33505:intron | 25.0% |
| !! | ACAACTCACAATTTCAATTT+TGG | + | chr3.4:82642889-82642908 | None:intergenic | 25.0% |
| !!! | TTTTGGTAACTATTGAAAAG+TGG | + | chr3.4:82642872-82642891 | None:intergenic | 25.0% |
| AGACATTCACCAGAATATTA+AGG | - | chr3.4:82642176-82642195 | MS.gene33505:intron | 30.0% | |
| GAACCATGTAACATAAAGAT+AGG | + | chr3.4:82642016-82642035 | None:intergenic | 30.0% | |
| GCTATTCTAACATGAACTAA+CGG | - | chr3.4:82643162-82643181 | MS.gene33505:intron | 30.0% | |
| TCAAGCTTACTAGAATACAA+TGG | + | chr3.4:82643333-82643352 | None:intergenic | 30.0% | |
| TTCTTGAAAAATTTCTGAGC+TGG | + | chr3.4:82642584-82642603 | None:intergenic | 30.0% | |
| ! | AAATCTGCATCTTTTCCATT+AGG | + | chr3.4:82643276-82643295 | None:intergenic | 30.0% |
| ! | AATCTGCATCTTTTCCATTA+GGG | + | chr3.4:82643275-82643294 | None:intergenic | 30.0% |
| ! | TCCAAGAAAGTTTTTGTATC+AGG | + | chr3.4:82643220-82643239 | None:intergenic | 30.0% |
| ! | TTTGCAGAAAGTGTATAACT+TGG | - | chr3.4:82643435-82643454 | MS.gene33505:CDS | 30.0% |
| !! | ATAGCTTTACTCTTTTGAAC+CGG | - | chr3.4:82643135-82643154 | MS.gene33505:intron | 30.0% |
| AAACAATGAACCACGATGTT+TGG | + | chr3.4:82643105-82643124 | None:intergenic | 35.0% | |
| AATGCAAAGAAGAGAGTTAG+CGG | - | chr3.4:82642520-82642539 | MS.gene33505:intron | 35.0% | |
| AATTTCGTTACCAAACATCG+TGG | - | chr3.4:82643092-82643111 | MS.gene33505:intron | 35.0% | |
| ACAATGCCCAACTTGTATTA+CGG | + | chr3.4:82643000-82643019 | None:intergenic | 35.0% | |
| ATTGTCTTGCAGATAAGGTA+GGG | - | chr3.4:82642130-82642149 | MS.gene33505:CDS | 35.0% | |
| CAATGGATATCCTCAAGAAA+AGG | + | chr3.4:82643316-82643335 | None:intergenic | 35.0% | |
| GCCTGATACAAAAACTTTCT+TGG | - | chr3.4:82643216-82643235 | MS.gene33505:intron | 35.0% | |
| GCTCATAGCTACTAATAAGT+GGG | - | chr3.4:82642930-82642949 | MS.gene33505:intron | 35.0% | |
| TCATCAGGTATGTATATGAC+AGG | + | chr3.4:82643403-82643422 | None:intergenic | 35.0% | |
| TGACATTGAACTGTTGAGTA+TGG | - | chr3.4:82642908-82642927 | MS.gene33505:intron | 35.0% | |
| TTCAAGTCAGAAACTAGCAA+AGG | - | chr3.4:82642496-82642515 | MS.gene33505:intron | 35.0% | |
| ! | AAAGATAGGAAAGGCTTTGT+TGG | + | chr3.4:82642002-82642021 | None:intergenic | 35.0% |
| ! | AGAAAGTGTATAACTTGGAC+AGG | - | chr3.4:82643440-82643459 | MS.gene33505:CDS | 35.0% |
| ! | CATTGATTGTCTTGCAGATA+AGG | - | chr3.4:82642125-82642144 | MS.gene33505:CDS | 35.0% |
| ! | GCTTGCTCCATATTCTTTTT+AGG | + | chr3.4:82642749-82642768 | None:intergenic | 35.0% |
| ! | TACATGGTTCTACAAGTTCT+TGG | - | chr3.4:82642026-82642045 | MS.gene33505:CDS | 35.0% |
| ! | TTTACTTGTGCCTTTTCTTG+AGG | - | chr3.4:82643303-82643322 | MS.gene33505:intron | 35.0% |
| !! | AAGATAGGAAAGGCTTTGTT+GGG | + | chr3.4:82642001-82642020 | None:intergenic | 35.0% |
| !! | ATGATGCAACTTTGAAACCA+TGG | - | chr3.4:82642783-82642802 | MS.gene33505:intron | 35.0% |
| AAATCCAGGGATCTACATAG+TGG | + | chr3.4:82642689-82642708 | None:intergenic | 40.0% | |
| ACAATGCTGATGTGAAATCC+AGG | + | chr3.4:82642703-82642722 | None:intergenic | 40.0% | |
| ACTAACGGAATTTGTACTGC+AGG | - | chr3.4:82643177-82643196 | MS.gene33505:CDS | 40.0% | |
| ATCAGGACCGTAATACAAGT+TGG | - | chr3.4:82642990-82643009 | MS.gene33505:intron | 40.0% | |
| CAAACCTGAAGAAGATCATG+AGG | - | chr3.4:82641962-82641981 | MS.gene33505:intron | 40.0% | |
| CAATGCTGATGTGAAATCCA+GGG | + | chr3.4:82642702-82642721 | None:intergenic | 40.0% | |
| CATCAATTGTTCATGCTCAC+TGG | - | chr3.4:82643373-82643392 | MS.gene33505:CDS | 40.0% | |
| CCAGAGGTAATGTTGTAATC+AGG | - | chr3.4:82642973-82642992 | MS.gene33505:intron | 40.0% | |
| CCTGATTACAACATTACCTC+TGG | + | chr3.4:82642976-82642995 | None:intergenic | 40.0% | |
| GATTGTCTTGCAGATAAGGT+AGG | - | chr3.4:82642129-82642148 | MS.gene33505:CDS | 40.0% | |
| GGCTCATAGCTACTAATAAG+TGG | - | chr3.4:82642929-82642948 | MS.gene33505:intron | 40.0% | |
| GTCATGTGTGTGTGAGTATT+TGG | + | chr3.4:82642205-82642224 | None:intergenic | 40.0% | |
| TCAGGACCGTAATACAAGTT+GGG | - | chr3.4:82642991-82643010 | MS.gene33505:intron | 40.0% | |
| TCATGTTAGAATAGCAACGC+CGG | + | chr3.4:82643157-82643176 | None:intergenic | 40.0% | |
| TGAGACAATATCGACACATG+TGG | + | chr3.4:82642284-82642303 | None:intergenic | 40.0% | |
| TGTCGATATTGTCTCACTGT+CGG | - | chr3.4:82642288-82642307 | MS.gene33505:intron | 40.0% | |
| TGTCTCAAAACAGCCATTGT+TGG | + | chr3.4:82642156-82642175 | None:intergenic | 40.0% | |
| ! | AATCCTTTGTTTGGCTCACT+AGG | - | chr3.4:82642548-82642567 | MS.gene33505:CDS | 40.0% |
| ! | AGTCTTCGTCTTTTTCTCTG+AGG | + | chr3.4:82643246-82643265 | None:intergenic | 40.0% |
| ! | TATGTGTCATTGCATTGCAG+AGG | - | chr3.4:82642419-82642438 | MS.gene33505:intron | 40.0% |
| !! | AAGCACTGAAAGCTCTTCAA+TGG | + | chr3.4:82641934-82641953 | None:intergenic | 40.0% |
| AACGGAATTTGTACTGCAGG+TGG | - | chr3.4:82643180-82643199 | MS.gene33505:CDS | 45.0% | |
| AGATAAGGTAGGGCCAACAA+TGG | - | chr3.4:82642140-82642159 | MS.gene33505:CDS | 45.0% | |
| GCGGCAGTAAATCCTTTGTT+TGG | - | chr3.4:82642539-82642558 | MS.gene33505:CDS | 45.0% | |
| TCACCTAGTGAGCCAAACAA+AGG | + | chr3.4:82642554-82642573 | None:intergenic | 45.0% | |
| TGTCATTGCATTGCAGAGGT+TGG | - | chr3.4:82642423-82642442 | MS.gene33505:intron | 45.0% | |
| !! | CTTTGAAACCATGGCATGGA+TGG | - | chr3.4:82642792-82642811 | MS.gene33505:intron | 45.0% |
| !! | GCAACTTTGAAACCATGGCA+TGG | - | chr3.4:82642788-82642807 | MS.gene33505:intron | 45.0% |
| CACTATGCAGTCAGAGACGT+AGG | - | chr3.4:82643057-82643076 | MS.gene33505:intron | 50.0% | |
| CCAGCCTCATGATCTTCTTC+AGG | + | chr3.4:82641969-82641988 | None:intergenic | 50.0% | |
| CCTGAAGAAGATCATGAGGC+TGG | - | chr3.4:82641966-82641985 | MS.gene33505:intron | 50.0% | |
| CGAAGACTGTGACACCCTAA+TGG | - | chr3.4:82643258-82643277 | MS.gene33505:intron | 50.0% | |
| GATGCAATCCATCCATGCCA+TGG | + | chr3.4:82642803-82642822 | None:intergenic | 50.0% | |
| GTCAGAGACGTAGGCACAAT+TGG | - | chr3.4:82643066-82643085 | MS.gene33505:intron | 50.0% | |
| ! | TGTGCCACTATGTAGATCCC+TGG | - | chr3.4:82642682-82642701 | MS.gene33505:intron | 50.0% |
| CATTACCTCTGGACCGTAGC+TGG | + | chr3.4:82642965-82642984 | None:intergenic | 55.0% | |
| CTCACTGTCGGACAAGAGAC+AGG | - | chr3.4:82642300-82642319 | MS.gene33505:intron | 55.0% | |
| TGGGCAAGTCGCTCCAGCTA+CGG | - | chr3.4:82642949-82642968 | MS.gene33505:intron | 60.0% | |
| TCGCTCCAGCTACGGTCCAG+AGG | - | chr3.4:82642957-82642976 | MS.gene33505:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 82641885 | 82643477 | 82641885 | ID=MS.gene33505 |
| chr3.4 | mRNA | 82641885 | 82643477 | 82641885 | ID=MS.gene33505.t1;Parent=MS.gene33505 |
| chr3.4 | exon | 82643315 | 82643477 | 82643315 | ID=MS.gene33505.t1.exon1;Parent=MS.gene33505.t1 |
| chr3.4 | CDS | 82643315 | 82643477 | 82643315 | ID=cds.MS.gene33505.t1;Parent=MS.gene33505.t1 |
| chr3.4 | exon | 82643165 | 82643220 | 82643165 | ID=MS.gene33505.t1.exon2;Parent=MS.gene33505.t1 |
| chr3.4 | CDS | 82643165 | 82643220 | 82643165 | ID=cds.MS.gene33505.t1;Parent=MS.gene33505.t1 |
| chr3.4 | exon | 82642793 | 82642923 | 82642793 | ID=MS.gene33505.t1.exon3;Parent=MS.gene33505.t1 |
| chr3.4 | CDS | 82642793 | 82642923 | 82642793 | ID=cds.MS.gene33505.t1;Parent=MS.gene33505.t1 |
| chr3.4 | exon | 82642527 | 82642665 | 82642527 | ID=MS.gene33505.t1.exon4;Parent=MS.gene33505.t1 |
| chr3.4 | CDS | 82642527 | 82642665 | 82642527 | ID=cds.MS.gene33505.t1;Parent=MS.gene33505.t1 |
| chr3.4 | exon | 82642020 | 82642163 | 82642020 | ID=MS.gene33505.t1.exon5;Parent=MS.gene33505.t1 |
| chr3.4 | CDS | 82642020 | 82642163 | 82642020 | ID=cds.MS.gene33505.t1;Parent=MS.gene33505.t1 |
| chr3.4 | exon | 82641885 | 82641920 | 82641885 | ID=MS.gene33505.t1.exon6;Parent=MS.gene33505.t1 |
| chr3.4 | CDS | 82641885 | 82641920 | 82641885 | ID=cds.MS.gene33505.t1;Parent=MS.gene33505.t1 |
| Gene Sequence |
| Protein sequence |
