AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074568


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074568.t1 MTR_6g018300 93.785 177 11 0 1 177 1 177 1.99e-126 352
MS.gene074568.t1 MTR_6g018310 91.667 180 12 1 1 177 1 180 7.57e-124 346
MS.gene074568.t1 MTR_7g007120 83.140 172 25 2 9 177 11 181 9.01e-106 300
MS.gene074568.t1 MTR_7g007120 82.558 172 26 2 9 177 11 181 4.37e-105 299
MS.gene074568.t1 MTR_7g007200 81.977 172 27 2 9 177 11 181 5.56e-105 298
MS.gene074568.t1 MTR_7g007220 81.977 172 27 2 9 177 11 181 1.03e-104 298
MS.gene074568.t1 MTR_7g007120 82.456 171 26 2 10 177 12 181 1.38e-104 298
MS.gene074568.t1 MTR_7g007230 80.702 171 29 2 10 177 12 181 1.59e-102 292
MS.gene074568.t1 MTR_7g007210 81.290 155 25 2 9 160 11 164 5.11e-91 264
MS.gene074568.t1 MTR_4g127870 50.000 156 75 2 23 176 15 169 2.59e-59 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074568.t1 AT1G67090 74.419 172 44 0 6 177 7 178 2.36e-99 284
MS.gene074568.t1 AT5G38410 73.295 176 46 1 2 177 4 178 8.92e-99 283
MS.gene074568.t1 AT5G38430 73.295 176 46 1 2 177 4 178 1.11e-98 283
MS.gene074568.t1 AT5G38430 73.295 176 46 1 2 177 4 178 1.11e-98 283
MS.gene074568.t1 AT5G38420 72.159 176 48 1 2 177 4 178 3.79e-97 279
MS.gene074568.t1 AT5G38410 71.271 181 46 2 2 177 4 183 2.77e-96 277
MS.gene074568.t1 AT5G38410 70.455 176 44 2 2 177 4 171 5.31e-92 265
MS.gene074568.t1 AT1G67090 65.714 105 36 0 6 110 7 111 3.24e-47 150

Find 59 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCAAACTTAGTAGGTTTC+TGG 0.166011 6.3:-14081683 None:intergenic
AATGTCATTGTTGGTCTTCT+TGG 0.291082 6.3:-14080574 None:intergenic
GAAGGGATGGATTCCTTGTT+TGG 0.310858 6.3:+14080978 MS.gene074568:CDS
TTGTTGGTCTTCTTGGTGAC+TGG 0.326813 6.3:-14080567 None:intergenic
AACTCATTCATCAGAATCAT+TGG 0.341056 6.3:+14081611 MS.gene074568:CDS
AGGAAGTAGAATACCTTATC+AGG 0.342754 6.3:+14080956 MS.gene074568:CDS
GATACTATGACGGACGTTAC+TGG 0.345500 6.3:+14081504 MS.gene074568:CDS
ATGATTCTGATGAATGAGTT+TGG 0.359780 6.3:-14081608 None:intergenic
TCAAACTTCTTCTTGCCAAC+TGG 0.385159 6.3:-14080889 None:intergenic
TGATTCTTCTCAAGTGTTGA+AGG 0.390177 6.3:+14081559 MS.gene074568:CDS
ATGTGGAGGTTGCCTTTGTT+CGG 0.417201 6.3:+14081530 MS.gene074568:CDS
ACCCCTCACTGAGGAACAAT+TGG 0.420148 6.3:+14080930 MS.gene074568:CDS
GAATCCATCCCTTCCTGATA+AGG 0.434100 6.3:-14080969 None:intergenic
GTGCAAATATCAAACTTAGT+AGG 0.441976 6.3:-14081690 None:intergenic
AGCCAATTGTTCCTCAGTGA+GGG 0.454137 6.3:-14080932 None:intergenic
TGTTGGTCTTCTTGGTGACT+GGG 0.456367 6.3:-14080566 None:intergenic
ACCGGTCTCAAATCCTCAGC+TGG 0.472739 6.3:+14080540 MS.gene074568:CDS
GTTAGCGGAGACGCGGTTAA+CGG 0.473759 6.3:-14080502 None:intergenic
TGATTCTGATGAATGAGTTT+GGG 0.474330 6.3:-14081607 None:intergenic
AACTTGGTTGCTCCATTCAC+CGG 0.481626 6.3:+14080522 MS.gene074568:CDS
TAACCGCGTCTCCGCTAACT+TGG 0.481938 6.3:+14080506 MS.gene074568:CDS
GAGGATTTGAGACCGGTGAA+TGG 0.484712 6.3:-14080534 None:intergenic
CGTGAGAACCACCGTTCACC+AGG 0.497088 6.3:+14081482 MS.gene074568:CDS
TCCACCATTGCTTGCAATGG+AGG 0.500934 6.3:-14080598 None:intergenic
AACGGTGGTAACAGCGGCGG+AGG 0.513043 6.3:-14080484 None:intergenic
TCTTCCACCATTGCTTGCAA+TGG 0.515588 6.3:-14080601 None:intergenic
TTGTTTGGAATTTGAGCTTG+AGG 0.518744 6.3:+14080993 MS.gene074568:CDS
AACTTCTTCTTGCCAACTGG+TGG 0.532490 6.3:-14080886 None:intergenic
AATGGAGGTAATGTCATTGT+TGG 0.536427 6.3:-14080583 None:intergenic
CACTGAGGAACAATTGGCTA+AGG 0.537360 6.3:+14080936 MS.gene074568:CDS
TAGCCAATTGTTCCTCAGTG+AGG 0.553733 6.3:-14080933 None:intergenic
TAACGTCCGTCATAGTATCC+TGG 0.560465 6.3:-14081500 None:intergenic
CTGGTGAACGGTGGTTCTCA+CGG 0.562589 6.3:-14081481 None:intergenic
ACCTCCATTGCAAGCAATGG+TGG 0.570031 6.3:+14080597 MS.gene074568:CDS
GTTCCTCAGTGAGGGGTGGA+AGG 0.571475 6.3:-14080924 None:intergenic
ACCAGCTGAGGATTTGAGAC+CGG 0.573385 6.3:-14080541 None:intergenic
GCCAATTGTTCCTCAGTGAG+GGG 0.576725 6.3:-14080931 None:intergenic
AATACCTTATCAGGAAGGGA+TGG 0.576828 6.3:+14080965 MS.gene074568:CDS
AGTAGAATACCTTATCAGGA+AGG 0.579075 6.3:+14080960 MS.gene074568:CDS
GAATCAGTGGCTCCGAACAA+AGG 0.592010 6.3:-14081542 None:intergenic
GAATGGAGCAACCAAGTTAG+CGG 0.594098 6.3:-14080517 None:intergenic
TTATAGGTGTGGCCACCAGT+TGG 0.602882 6.3:+14080874 MS.gene074568:intron
ATTACCTCCATTGCAAGCAA+TGG 0.615000 6.3:+14080594 MS.gene074568:CDS
TCAAGCTCAAATTCCAAACA+AGG 0.620566 6.3:-14080991 None:intergenic
CAACCAAGTTAGCGGAGACG+CGG 0.626784 6.3:-14080509 None:intergenic
GTAGAATACCTTATCAGGAA+GGG 0.631047 6.3:+14080961 MS.gene074568:CDS
TGGAAGAGTGAACTGTATGC+AGG 0.631476 6.3:+14080617 MS.gene074568:CDS
CAACACTTGAGAAGAATCAG+TGG 0.638041 6.3:-14081555 None:intergenic
CGTCATAGTATCCTGGTGAA+CGG 0.638410 6.3:-14081493 None:intergenic
AATTGTTCCTCAGTGAGGGG+TGG 0.640814 6.3:-14080928 None:intergenic
GGTGACTGGGAAACCAGCTG+AGG 0.655087 6.3:-14080553 None:intergenic
CATAGTATCCTGGTGAACGG+TGG 0.657211 6.3:-14081490 None:intergenic
GCGGTTAACGGTGGTAACAG+CGG 0.666203 6.3:-14080490 None:intergenic
GACGTTACTGGACAATGTGG+AGG 0.672886 6.3:+14081516 MS.gene074568:CDS
AGCGGAGACGCGGTTAACGG+TGG 0.674421 6.3:-14080499 None:intergenic
GTTAACGGTGGTAACAGCGG+CGG 0.699396 6.3:-14080487 None:intergenic
CGTTCACCAGGATACTATGA+CGG 0.703631 6.3:+14081494 MS.gene074568:CDS
ACGGACGTTACTGGACAATG+TGG 0.708236 6.3:+14081513 MS.gene074568:CDS
ATACCTTCCACCCCTCACTG+AGG 0.719780 6.3:+14080921 MS.gene074568:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATATATACAATAAAG+TGG - chr6.3:14081134-14081153 None:intergenic 10.0%
!! ATGTCATAAAAAAATAAAAT+TGG + chr6.3:14080745-14080764 MS.gene074568:intron 10.0%
!!! ACATGAAATTAAATTTTATA+CGG - chr6.3:14080680-14080699 None:intergenic 10.0%
!!! ATATGTAAAATATTTTTACA+CGG - chr6.3:14081205-14081224 None:intergenic 10.0%
!!! ATTTTATGTATGATATTTAT+AGG + chr6.3:14080858-14080877 MS.gene074568:intron 10.0%
!! AAACACTATAAAAATATCAT+AGG - chr6.3:14081243-14081262 None:intergenic 15.0%
!! ATACATAAAATTGAGTATTA+GGG - chr6.3:14080849-14080868 None:intergenic 15.0%
!! ATTAAACTCGTAAATTAAAA+AGG - chr6.3:14081049-14081068 None:intergenic 15.0%
!! GTAAATTAAAAAGGAAAAAA+AGG - chr6.3:14081040-14081059 None:intergenic 15.0%
!! CATACATAAAATTGAGTATT+AGG - chr6.3:14080850-14080869 None:intergenic 20.0%
!! TCATAATTTAAGTAACCTTA+AGG - chr6.3:14080722-14080741 None:intergenic 20.0%
!! TTAATTAATTGTGTCAACTT+TGG - chr6.3:14080807-14080826 None:intergenic 20.0%
!! TTACATATTTATGTACCTTA+AGG + chr6.3:14080704-14080723 MS.gene074568:intron 20.0%
!!! ATTTGTTGTGTTATATAGAA+AGG + chr6.3:14081449-14081468 MS.gene074568:intron 20.0%
!!! GATATTTTTATAGTGTTTAG+TGG + chr6.3:14081245-14081264 MS.gene074568:intron 20.0%
! ATACGGTATATGCAAAAATA+TGG - chr6.3:14080663-14080682 None:intergenic 25.0%
! ATGTATGATATTTATAGGTG+TGG + chr6.3:14080863-14080882 MS.gene074568:intron 25.0%
! TATATGCAAAAATATGGACT+AGG - chr6.3:14080657-14080676 None:intergenic 25.0%
! TTATAGTGTTTAGTGGAATT+TGG + chr6.3:14081252-14081271 MS.gene074568:intron 25.0%
!! ATATGCAAAAATATGGACTA+GGG - chr6.3:14080656-14080675 None:intergenic 25.0%
AAAGGAAAAAAAGGTTGCAA+TGG - chr6.3:14081031-14081050 None:intergenic 30.0%
AACTCATTCATCAGAATCAT+TGG + chr6.3:14081611-14081630 MS.gene074568:CDS 30.0%
ATGATTCTGATGAATGAGTT+TGG - chr6.3:14081611-14081630 None:intergenic 30.0%
TGATTCTGATGAATGAGTTT+GGG - chr6.3:14081610-14081629 None:intergenic 30.0%
AATGGAGGTAATGTCATTGT+TGG - chr6.3:14080586-14080605 None:intergenic 35.0%
AGGAAGTAGAATACCTTATC+AGG + chr6.3:14080956-14080975 MS.gene074568:CDS 35.0%
AGTAGAATACCTTATCAGGA+AGG + chr6.3:14080960-14080979 MS.gene074568:CDS 35.0%
CAGGTTGTTGGAATTTGATA+TGG + chr6.3:14081318-14081337 MS.gene074568:intron 35.0%
GCGTCATGATAAAATCTGAT+TGG - chr6.3:14081112-14081131 None:intergenic 35.0%
GTAGAATACCTTATCAGGAA+GGG + chr6.3:14080961-14080980 MS.gene074568:CDS 35.0%
TCAAGCTCAAATTCCAAACA+AGG - chr6.3:14080994-14081013 None:intergenic 35.0%
! TTGTTTGGAATTTGAGCTTG+AGG + chr6.3:14080993-14081012 MS.gene074568:CDS 35.0%
!! AATGTCATTGTTGGTCTTCT+TGG - chr6.3:14080577-14080596 None:intergenic 35.0%
!! TGATTCTTCTCAAGTGTTGA+AGG + chr6.3:14081559-14081578 MS.gene074568:CDS 35.0%
AATACCTTATCAGGAAGGGA+TGG + chr6.3:14080965-14080984 MS.gene074568:CDS 40.0%
AATCCTCTCATTCACTCAGT+CGG + chr6.3:14081291-14081310 MS.gene074568:intron 40.0%
ACCCTACGAAATTCATCACT+CGG - chr6.3:14081419-14081438 None:intergenic 40.0%
ATTACCTCCATTGCAAGCAA+TGG + chr6.3:14080594-14080613 MS.gene074568:CDS 40.0%
CAACACTTGAGAAGAATCAG+TGG - chr6.3:14081558-14081577 None:intergenic 40.0%
TCAAACTTCTTCTTGCCAAC+TGG - chr6.3:14080892-14080911 None:intergenic 40.0%
TCAGCACATCATGTAGTTGT+TGG - chr6.3:14081346-14081365 None:intergenic 40.0%
TCCGAGTGATGAATTTCGTA+GGG + chr6.3:14081415-14081434 MS.gene074568:intron 40.0%
TTCCGAGTGATGAATTTCGT+AGG + chr6.3:14081414-14081433 MS.gene074568:intron 40.0%
! ATTCGGATTGTCTGAAGTCA+AGG - chr6.3:14081389-14081408 None:intergenic 40.0%
AACTTCTTCTTGCCAACTGG+TGG - chr6.3:14080889-14080908 None:intergenic 45.0%
AACTTGGTTGCTCCATTCAC+CGG + chr6.3:14080522-14080541 MS.gene074568:CDS 45.0%
AGCCAATTGTTCCTCAGTGA+GGG - chr6.3:14080935-14080954 None:intergenic 45.0%
ATGTGGAGGTTGCCTTTGTT+CGG + chr6.3:14081530-14081549 MS.gene074568:CDS 45.0%
CACTGAGGAACAATTGGCTA+AGG + chr6.3:14080936-14080955 MS.gene074568:CDS 45.0%
CATCACTCGGAACACGAATT+CGG - chr6.3:14081406-14081425 None:intergenic 45.0%
CGTCATAGTATCCTGGTGAA+CGG - chr6.3:14081496-14081515 None:intergenic 45.0%
CGTTCACCAGGATACTATGA+CGG + chr6.3:14081494-14081513 MS.gene074568:CDS 45.0%
GAATCCATCCCTTCCTGATA+AGG - chr6.3:14080972-14080991 None:intergenic 45.0%
GAATGGAGCAACCAAGTTAG+CGG - chr6.3:14080520-14080539 None:intergenic 45.0%
TAACGTCCGTCATAGTATCC+TGG - chr6.3:14081503-14081522 None:intergenic 45.0%
TAGCCAATTGTTCCTCAGTG+AGG - chr6.3:14080936-14080955 None:intergenic 45.0%
TCTTCCACCATTGCTTGCAA+TGG - chr6.3:14080604-14080623 None:intergenic 45.0%
TGGAAGAGTGAACTGTATGC+AGG + chr6.3:14080617-14080636 MS.gene074568:CDS 45.0%
! ATGAGAGGATTATCGACTGC+AGG - chr6.3:14081282-14081301 None:intergenic 45.0%
! GAAGGGATGGATTCCTTGTT+TGG + chr6.3:14080978-14080997 MS.gene074568:CDS 45.0%
!! ATGTGCTGACTCTGTGAATG+CGG + chr6.3:14081357-14081376 MS.gene074568:intron 45.0%
!! GATACTATGACGGACGTTAC+TGG + chr6.3:14081504-14081523 MS.gene074568:CDS 45.0%
!! TGTTGGTCTTCTTGGTGACT+GGG - chr6.3:14080569-14080588 None:intergenic 45.0%
!! TTGTTGGTCTTCTTGGTGAC+TGG - chr6.3:14080570-14080589 None:intergenic 45.0%
AATTGTTCCTCAGTGAGGGG+TGG - chr6.3:14080931-14080950 None:intergenic 50.0%
ACCCCTCACTGAGGAACAAT+TGG + chr6.3:14080930-14080949 MS.gene074568:CDS 50.0%
ACCTCCATTGCAAGCAATGG+TGG + chr6.3:14080597-14080616 MS.gene074568:CDS 50.0%
ACGGACGTTACTGGACAATG+TGG + chr6.3:14081513-14081532 MS.gene074568:CDS 50.0%
CATAGTATCCTGGTGAACGG+TGG - chr6.3:14081493-14081512 None:intergenic 50.0%
CATTCACTCAGTCGGTACAC+AGG + chr6.3:14081299-14081318 MS.gene074568:intron 50.0%
GAATCAGTGGCTCCGAACAA+AGG - chr6.3:14081545-14081564 None:intergenic 50.0%
GACGTTACTGGACAATGTGG+AGG + chr6.3:14081516-14081535 MS.gene074568:CDS 50.0%
GCCAATTGTTCCTCAGTGAG+GGG - chr6.3:14080934-14080953 None:intergenic 50.0%
TCAGTCGGTACACAGGTTGT+TGG + chr6.3:14081306-14081325 MS.gene074568:intron 50.0%
TCCACCATTGCTTGCAATGG+AGG - chr6.3:14080601-14080620 None:intergenic 50.0%
TTATAGGTGTGGCCACCAGT+TGG + chr6.3:14080874-14080893 MS.gene074568:intron 50.0%
! ACCAGCTGAGGATTTGAGAC+CGG - chr6.3:14080544-14080563 None:intergenic 50.0%
! GAGGATTTGAGACCGGTGAA+TGG - chr6.3:14080537-14080556 None:intergenic 50.0%
!! GTACCGACTGAGTGAATGAG+AGG - chr6.3:14081297-14081316 None:intergenic 50.0%
ACCGGTCTCAAATCCTCAGC+TGG + chr6.3:14080540-14080559 MS.gene074568:CDS 55.0%
ATACCTTCCACCCCTCACTG+AGG + chr6.3:14080921-14080940 MS.gene074568:CDS 55.0%
CAACCAAGTTAGCGGAGACG+CGG - chr6.3:14080512-14080531 None:intergenic 55.0%
CTGGTGAACGGTGGTTCTCA+CGG - chr6.3:14081484-14081503 None:intergenic 55.0%
GCGGTTAACGGTGGTAACAG+CGG - chr6.3:14080493-14080512 None:intergenic 55.0%
GTTAACGGTGGTAACAGCGG+CGG - chr6.3:14080490-14080509 None:intergenic 55.0%
GTTAGCGGAGACGCGGTTAA+CGG - chr6.3:14080505-14080524 None:intergenic 55.0%
TAACCGCGTCTCCGCTAACT+TGG + chr6.3:14080506-14080525 MS.gene074568:CDS 55.0%
CGTGAGAACCACCGTTCACC+AGG + chr6.3:14081482-14081501 MS.gene074568:CDS 60.0%
GGTGACTGGGAAACCAGCTG+AGG - chr6.3:14080556-14080575 None:intergenic 60.0%
GTTCCTCAGTGAGGGGTGGA+AGG - chr6.3:14080927-14080946 None:intergenic 60.0%
AACGGTGGTAACAGCGGCGG+AGG - chr6.3:14080487-14080506 None:intergenic 65.0%
AGCGGAGACGCGGTTAACGG+TGG - chr6.3:14080502-14080521 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 14080471 14081697 14080471 ID=MS.gene074568
chr6.3 mRNA 14080471 14081697 14080471 ID=MS.gene074568.t1;Parent=MS.gene074568
chr6.3 exon 14080471 14080638 14080471 ID=MS.gene074568.t1.exon1;Parent=MS.gene074568.t1
chr6.3 CDS 14080471 14080638 14080471 ID=cds.MS.gene074568.t1;Parent=MS.gene074568.t1
chr6.3 exon 14080880 14081014 14080880 ID=MS.gene074568.t1.exon2;Parent=MS.gene074568.t1
chr6.3 CDS 14080880 14081014 14080880 ID=cds.MS.gene074568.t1;Parent=MS.gene074568.t1
chr6.3 exon 14081467 14081697 14081467 ID=MS.gene074568.t1.exon3;Parent=MS.gene074568.t1
chr6.3 CDS 14081467 14081697 14081467 ID=cds.MS.gene074568.t1;Parent=MS.gene074568.t1
Gene Sequence

>MS.gene074568

ATGGCTTTGATCTCCTCCGCCGCTGTTACCACCGTTAACCGCGTCTCCGCTAACTTGGTTGCTCCATTCACCGGTCTCAAATCCTCAGCTGGTTTCCCAGTCACCAAGAAGACCAACAATGACATTACCTCCATTGCAAGCAATGGTGGAAGAGTGAACTGTATGCAGGTAATACACACAAGCCCTAGTCCATATTTTTGCATATACCGTATAAAATTTAATTTCATGTTATGTTACATATTTATGTACCTTAAGGTTACTTAAATTATGAATCATGTCATAAAAAAATAAAATTGGACTATAATTCTAGATATATTTTATCTACAAAGTATACCAAAGTTGACACAATTAATTAAAATTGTCAAATTTTGTTAACCCTAATACTCAATTTTATGTATGATATTTATAGGTGTGGCCACCAGTTGGCAAGAAGAAGTTTGAGACACTTTCATACCTTCCACCCCTCACTGAGGAACAATTGGCTAAGGAAGTAGAATACCTTATCAGGAAGGGATGGATTCCTTGTTTGGAATTTGAGCTTGAGGTTAATCTATTTACCATTGCAACCTTTTTTTCCTTTTTAATTTACGAGTTTAATTACTATACACAGACAATGAAAAACTTTTTTACATTTACAACCAATCAGATTTTATCATGACGCCACTTTATTGTATATATTTATTCTTAAAATATCTCAACAAGCATTTACGACGCAAAATCTGATTGAATAACCGTGTAAAAATATTTTACATATATAGTTAATATATAGCCTATGATATTTTTATAGTGTTTAGTGGAATTTGGATTACCTGCAGTCGATAATCCTCTCATTCACTCAGTCGGTACACAGGTTGTTGGAATTTGATATGGATCCAACAACTACATGATGTGCTGACTCTGTGAATGCGGATAATTCCTTGACTTCAGACAATCCGAATTCGTGTTCCGAGTGATGAATTTCGTAGGGTTGATGTGTTAATTTGTTGTGTTATATAGAAAGGATTTGTCTACCGTGAGAACCACCGTTCACCAGGATACTATGACGGACGTTACTGGACAATGTGGAGGTTGCCTTTGTTCGGAGCCACTGATTCTTCTCAAGTGTTGAAGGAGCTTGCTGACTGTAAAGCTGAATACCCAAACTCATTCATCAGAATCATTGGATTTGACAATGTTCGTCAAGTGCAGTGCATTAGTTTCATTGCACACACACCAGAAACCTACTAA

Protein sequence

>MS.gene074568.t1

MALISSAAVTTVNRVSANLVAPFTGLKSSAGFPVTKKTNNDITSIASNGGRVNCMQVWPPVGKKKFETLSYLPPLTEEQLAKEVEYLIRKGWIPCLEFELEKGFVYRENHRSPGYYDGRYWTMWRLPLFGATDSSQVLKELADCKAEYPNSFIRIIGFDNVRQVQCISFIAHTPETY