AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054821

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054821.t1 MTR_1g100180 60.656 488 192 0 43 530 8 495 0.0 615
MS.gene054821.t1 MTR_6g047610 50.851 470 230 1 58 527 11 479 1.87e-179 514
MS.gene054821.t1 MTR_6g047640 49.787 470 236 0 58 527 11 480 1.77e-167 483
MS.gene054821.t1 MTR_1g021740 48.889 495 235 3 30 523 4 481 2.20e-166 481
MS.gene054821.t1 MTR_6g047590 50.427 468 231 1 58 525 11 477 8.73e-164 473
MS.gene054821.t1 MTR_6g047640 47.872 470 226 1 58 527 11 461 3.25e-162 469
MS.gene054821.t1 MTR_8g069470 47.358 492 252 3 46 530 13 504 5.18e-162 470
MS.gene054821.t1 MTR_5g033100 49.462 465 234 1 62 525 40 504 9.82e-157 457
MS.gene054821.t1 MTR_5g032720 48.696 460 234 2 70 527 28 487 7.76e-144 423
MS.gene054821.t1 MTR_7g087410 45.312 448 244 1 79 525 32 479 8.12e-141 416
MS.gene054821.t1 MTR_7g087400 45.336 461 252 0 70 530 22 482 5.79e-140 413
MS.gene054821.t1 MTR_2g088400 42.942 503 279 4 27 527 7 503 2.15e-138 410
MS.gene054821.t1 MTR_4g048000 44.191 482 266 2 49 528 36 516 6.10e-136 404
MS.gene054821.t1 MTR_6g027200 46.137 453 243 1 70 521 28 480 7.17e-134 398
MS.gene054821.t1 MTR_3g099700 43.254 504 270 4 21 522 3 492 3.75e-133 396
MS.gene054821.t1 MTR_2g022980 43.121 487 265 3 47 521 7 493 2.51e-132 394
MS.gene054821.t1 MTR_5g032750 43.182 484 268 4 41 521 2 481 1.70e-128 384
MS.gene054821.t1 MTR_7g087410 47.179 390 205 1 137 525 1 390 1.22e-126 376
MS.gene054821.t1 MTR_6g027190 43.074 462 262 1 70 530 16 477 9.66e-124 372
MS.gene054821.t1 MTR_7g087430 45.995 387 208 1 137 522 1 387 4.00e-121 362
MS.gene054821.t1 MTR_7g087370 43.142 452 228 1 70 521 22 444 9.38e-121 363
MS.gene054821.t1 MTR_3g099720 43.243 444 251 1 78 520 41 484 8.88e-120 362
MS.gene054821.t1 MTR_2g088400 43.333 450 247 4 27 474 7 450 9.35e-118 355
MS.gene054821.t1 MTR_5g090290 42.029 483 263 3 40 520 23 490 1.28e-114 351
MS.gene054821.t1 MTR_8g063590 43.421 456 252 5 79 530 54 507 3.97e-112 342
MS.gene054821.t1 MTR_5g087930 38.105 475 255 10 57 525 15 456 7.49e-91 286
MS.gene054821.t1 MTR_6g081410 34.934 458 293 3 67 520 15 471 5.43e-84 269
MS.gene054821.t1 MTR_6g066380 35.828 441 278 3 82 518 32 471 4.11e-79 256
MS.gene054821.t1 MTR_2g078930 35.095 473 299 3 45 509 4 476 1.06e-78 255
MS.gene054821.t1 MTR_6g081780 33.619 467 305 3 67 529 15 480 1.63e-78 254
MS.gene054821.t1 MTR_6g081240 35.811 444 270 8 77 511 8 445 6.51e-78 252
MS.gene054821.t1 MTR_6g081300 33.853 449 289 4 76 519 8 453 1.02e-76 249
MS.gene054821.t1 MTR_1g108810 36.117 443 272 5 77 512 32 470 4.19e-76 248
MS.gene054821.t1 MTR_7g082810 32.819 454 303 1 79 530 21 474 1.49e-75 247
MS.gene054821.t1 MTR_2g079020 36.322 435 275 1 77 509 36 470 2.45e-75 247
MS.gene054821.t1 MTR_2g078960 36.508 441 275 3 73 509 27 466 9.09e-74 242
MS.gene054821.t1 MTR_8g106670 32.018 456 302 3 70 520 4 456 1.14e-71 236
MS.gene054821.t1 MTR_6g081810 33.630 449 273 3 70 518 20 443 1.15e-71 236
MS.gene054821.t1 MTR_2g078970 34.862 436 280 2 77 509 30 464 6.45e-71 235
MS.gene054821.t1 MTR_6g081390 32.684 462 298 6 70 525 8 462 1.26e-69 231
MS.gene054821.t1 MTR_1g108840 31.070 486 327 2 35 518 4 483 2.16e-69 231
MS.gene054821.t1 MTR_6g081400 31.897 464 302 7 70 526 11 467 1.70e-68 228
MS.gene054821.t1 MTR_5g090280 35.407 418 234 4 137 525 1 411 2.65e-67 223
MS.gene054821.t1 MTR_6g004220 31.209 455 310 2 67 518 12 466 2.84e-66 222
MS.gene054821.t1 MTR_6g081310 35.963 431 254 7 106 518 24 450 3.82e-65 219
MS.gene054821.t1 MTR_1g108990 34.110 472 302 4 53 520 3 469 3.92e-64 217
MS.gene054821.t1 MTR_6g004220 31.250 448 305 2 67 511 19 466 1.26e-63 216
MS.gene054821.t1 MTR_1g109060 33.559 444 293 1 86 527 32 475 3.54e-63 214
MS.gene054821.t1 MTR_1g109060 33.718 433 285 1 86 516 32 464 1.10e-60 207
MS.gene054821.t1 MTR_7g082800 30.303 396 271 2 137 530 1 393 1.41e-57 197
MS.gene054821.t1 MTR_1g101500 28.600 500 348 4 27 523 14 507 5.59e-57 199
MS.gene054821.t1 MTR_5g010830 27.955 440 315 1 79 516 66 505 5.77e-57 199
MS.gene054821.t1 MTR_0039s0080 28.412 447 308 3 86 527 46 485 3.20e-54 191
MS.gene054821.t1 MTR_5g067460 26.829 451 328 1 77 525 54 504 4.64e-52 186
MS.gene054821.t1 MTR_3g111020 28.097 452 321 2 74 522 47 497 9.27e-51 182
MS.gene054821.t1 MTR_6g043810 26.201 458 321 5 73 516 28 482 5.56e-48 174
MS.gene054821.t1 MTR_6g043810 26.201 458 321 5 73 516 18 472 5.64e-48 174
MS.gene054821.t1 MTR_2g078990 35.274 292 185 2 221 509 3 293 1.23e-47 168
MS.gene054821.t1 MTR_6g088805 26.116 448 330 1 67 514 16 462 1.78e-47 172
MS.gene054821.t1 MTR_4g027920 26.386 451 328 2 79 525 157 607 6.33e-47 173
MS.gene054821.t1 MTR_6g004220 29.024 379 266 2 67 442 19 397 1.47e-41 155
MS.gene054821.t1 MTR_8g102430 25.000 456 320 4 60 514 7 441 2.48e-39 149
MS.gene054821.t1 MTR_2g079030 37.949 195 121 0 315 509 319 513 2.84e-35 139
MS.gene054821.t1 MTR_2g435930 24.675 462 330 5 73 516 14 475 2.45e-34 136
MS.gene054821.t1 MTR_5g011540 26.395 466 328 5 56 515 27 483 6.36e-34 135
MS.gene054821.t1 MTR_6g004220 30.526 285 195 2 67 348 19 303 1.87e-30 121
MS.gene054821.t1 MTR_4g131290 49.206 63 32 0 148 210 11 73 1.08e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054821.t1 AT4G00350 72.167 503 136 3 23 521 28 530 0.0 746
MS.gene054821.t1 AT4G25640 65.319 470 163 0 62 531 17 486 0.0 611
MS.gene054821.t1 AT4G25640 65.319 470 163 0 62 531 17 486 0.0 610
MS.gene054821.t1 AT4G25640 66.348 419 141 0 62 480 17 435 0.0 558
MS.gene054821.t1 AT1G47530 53.333 450 209 1 79 527 35 484 9.65e-166 479
MS.gene054821.t1 AT1G12950 49.036 467 237 1 62 527 50 516 1.47e-159 464
MS.gene054821.t1 AT1G23300 47.976 494 250 4 40 531 9 497 1.01e-157 459
MS.gene054821.t1 AT3G26590 47.679 474 247 1 59 531 25 498 1.79e-157 458
MS.gene054821.t1 AT5G38030 45.875 497 263 3 35 530 3 494 3.66e-157 457
MS.gene054821.t1 AT1G47530 54.592 392 177 1 137 527 1 392 5.76e-146 425
MS.gene054821.t1 AT3G21690 47.619 462 240 1 69 528 42 503 4.38e-144 424
MS.gene054821.t1 AT5G65380 49.451 455 228 2 70 522 28 482 1.89e-139 412
MS.gene054821.t1 AT3G26590 49.747 396 198 1 137 531 1 396 6.16e-138 405
MS.gene054821.t1 AT1G11670 44.760 458 251 1 66 521 37 494 9.08e-137 405
MS.gene054821.t1 AT1G61890 44.396 455 251 1 69 521 37 491 9.44e-136 403
MS.gene054821.t1 AT1G33110 43.956 455 254 1 72 525 29 483 2.04e-129 386
MS.gene054821.t1 AT1G61890 43.946 446 248 1 69 512 37 482 2.82e-129 386
MS.gene054821.t1 AT1G33100 43.653 449 252 1 78 525 32 480 6.68e-127 380
MS.gene054821.t1 AT5G44050 45.815 454 244 2 71 522 32 485 8.39e-127 380
MS.gene054821.t1 AT1G33080 43.006 479 267 2 53 525 5 483 1.99e-126 379
MS.gene054821.t1 AT1G33090 44.396 455 252 1 72 525 29 483 8.10e-126 377
MS.gene054821.t1 AT4G21910 40.000 515 296 5 18 530 4 507 5.33e-123 370
MS.gene054821.t1 AT4G21910 40.000 515 296 5 18 530 4 507 5.33e-123 370
MS.gene054821.t1 AT1G33080 43.284 469 260 2 53 515 5 473 9.87e-123 369
MS.gene054821.t1 AT4G21910 42.857 455 257 2 78 530 56 509 3.05e-121 366
MS.gene054821.t1 AT4G21910 42.857 455 257 2 78 530 56 509 3.05e-121 366
MS.gene054821.t1 AT4G21903 43.187 433 243 2 95 525 69 500 5.56e-121 365
MS.gene054821.t1 AT3G59030 41.189 454 265 1 79 530 51 504 6.16e-118 357
MS.gene054821.t1 AT5G10420 47.085 446 234 2 78 521 37 482 5.09e-117 354
MS.gene054821.t1 AT1G61890 43.627 408 228 1 69 474 37 444 1.40e-115 350
MS.gene054821.t1 AT4G21903 42.757 428 242 2 95 520 69 495 1.89e-115 351
MS.gene054821.t1 AT4G21903 43.990 391 217 1 137 525 1 391 1.41e-114 345
MS.gene054821.t1 AT5G17700 39.101 445 270 1 79 522 34 478 6.28e-105 323
MS.gene054821.t1 AT5G17700 39.101 445 270 1 79 522 34 478 6.28e-105 323
MS.gene054821.t1 AT3G03620 38.202 445 274 1 79 522 37 481 1.72e-101 315
MS.gene054821.t1 AT3G03620 38.202 445 274 1 79 522 37 481 1.72e-101 315
MS.gene054821.t1 AT1G33110 43.200 375 212 1 72 445 29 403 1.76e-96 299
MS.gene054821.t1 AT3G59030 39.071 366 221 1 79 442 51 416 8.34e-83 264
MS.gene054821.t1 AT5G52450 34.694 441 285 2 82 520 30 469 6.19e-77 251
MS.gene054821.t1 AT1G71140 34.382 477 297 5 48 518 1 467 1.68e-76 249
MS.gene054821.t1 AT2G04080 33.708 445 293 1 76 518 24 468 8.15e-76 247
MS.gene054821.t1 AT1G73700 36.623 456 278 5 70 518 14 465 7.05e-75 245
MS.gene054821.t1 AT2G04070 33.860 443 291 1 78 518 26 468 2.51e-74 243
MS.gene054821.t1 AT2G04040 34.513 452 286 4 73 518 21 468 8.64e-74 242
MS.gene054821.t1 AT2G04080 33.713 439 289 1 76 512 24 462 3.48e-73 240
MS.gene054821.t1 AT2G34360 33.839 461 289 6 66 520 14 464 4.16e-73 240
MS.gene054821.t1 AT5G17700 36.141 368 234 1 79 445 34 401 1.15e-72 237
MS.gene054821.t1 AT3G23560 33.621 464 297 5 55 509 10 471 2.85e-71 235
MS.gene054821.t1 AT1G15150 33.407 455 301 1 79 531 31 485 1.03e-70 234
MS.gene054821.t1 AT2G04050 33.708 445 293 1 76 518 24 468 2.39e-70 233
MS.gene054821.t1 AT1G15160 32.967 455 303 1 79 531 31 485 1.86e-69 231
MS.gene054821.t1 AT2G04090 32.816 451 298 3 79 527 30 477 1.32e-68 228
MS.gene054821.t1 AT1G66780 32.494 437 283 4 97 526 53 484 3.39e-68 228
MS.gene054821.t1 AT3G23550 33.787 441 285 3 74 509 25 463 1.78e-67 225
MS.gene054821.t1 AT1G15170 33.183 443 294 1 78 518 33 475 2.66e-66 223
MS.gene054821.t1 AT2G04100 32.579 442 296 2 79 518 30 471 2.66e-65 220
MS.gene054821.t1 AT1G66760 30.519 462 316 2 70 526 19 480 8.13e-65 219
MS.gene054821.t1 AT2G04100 32.798 436 291 2 79 512 30 465 1.51e-63 215
MS.gene054821.t1 AT1G66760 30.631 444 306 1 70 511 19 462 7.28e-63 213
MS.gene054821.t1 AT2G34360 31.155 459 260 4 66 519 14 421 2.96e-61 208
MS.gene054821.t1 AT1G64820 28.604 444 311 2 85 524 35 476 2.54e-60 207
MS.gene054821.t1 AT5G19700 31.293 441 299 2 79 516 36 475 3.68e-60 207
MS.gene054821.t1 AT1G15180 31.498 454 308 2 68 518 23 476 1.51e-58 202
MS.gene054821.t1 AT1G15150 33.811 349 229 1 185 531 21 369 8.05e-53 184
MS.gene054821.t1 AT1G15170 33.929 336 220 1 185 518 21 356 1.10e-52 183
MS.gene054821.t1 AT4G29140 26.652 469 328 2 62 514 25 493 5.13e-52 186
MS.gene054821.t1 AT2G38510 26.411 443 319 4 79 516 6 446 7.57e-51 181
MS.gene054821.t1 AT3G23560 32.242 397 258 5 55 442 10 404 4.26e-50 177
MS.gene054821.t1 AT1G58340 25.843 445 328 1 86 528 65 509 1.74e-45 167
MS.gene054821.t1 AT1G66760 30.539 334 230 1 180 511 1 334 4.08e-45 162
MS.gene054821.t1 AT1G71870 25.979 485 320 8 76 529 21 497 1.02e-42 159
MS.gene054821.t1 AT4G23030 28.404 426 302 2 98 520 51 476 2.99e-41 155
MS.gene054821.t1 AT5G52050 25.000 468 327 4 64 515 20 479 9.70e-41 154
MS.gene054821.t1 AT1G66760 28.267 375 267 1 70 442 19 393 7.57e-40 149
MS.gene054821.t1 AT5G49130 24.401 459 327 6 76 516 23 479 3.09e-38 147
MS.gene054821.t1 AT4G22790 28.604 437 298 6 102 525 53 488 1.92e-35 139
MS.gene054821.t1 AT1G15180 30.385 260 179 1 185 442 21 280 5.22e-29 119
MS.gene054821.t1 AT2G04066 30.894 123 85 0 396 518 41 163 5.58e-18 82.0

Find 99 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATTGTTTCAGGGTATT+TGG 0.153563 7.2:+27548402 MS.gene054821:intron
CATAATATTCCAAAACAAAT+AGG 0.199558 7.2:-27545547 None:intergenic
AGCTATTATGCTTTGTTTAG+AGG 0.245160 7.2:+27546561 MS.gene054821:CDS
CGTTCAATTCTAATCTTATT+CGG 0.297281 7.2:+27546121 MS.gene054821:CDS
CCGTTGGCTCACTTTCAATA+TGG 0.300115 7.2:+27546641 MS.gene054821:CDS
ATTGCAAGCCCTATTTGTTT+TGG 0.303655 7.2:+27545538 MS.gene054821:CDS
TTATGCTTTGTTTAGAGGTT+TGG 0.311619 7.2:+27546566 MS.gene054821:CDS
TGTTGAATCGGCGAGGCTTT+GGG 0.314302 7.2:+27545513 MS.gene054821:CDS
ACCAATGTTGGAGACTATTC+TGG 0.317278 7.2:+27545454 MS.gene054821:CDS
TCTAAGTTAGCATCTCTTCT+TGG 0.320396 7.2:+27547749 MS.gene054821:CDS
AGCTTATGATTTATCAGCTT+GGG 0.329059 7.2:+27546423 MS.gene054821:CDS
ACAAAGCAAAGTTGGAGTTT+TGG 0.340262 7.2:+27546309 MS.gene054821:CDS
GTTGTTTCAAACTTCTCTTT+TGG 0.345170 7.2:+27545655 MS.gene054821:CDS
GTTGTTGAATCTCTCATCAT+TGG 0.354682 7.2:+27547641 MS.gene054821:CDS
GCTCAAATGGTTTATGTTGT+TGG 0.357374 7.2:+27546457 MS.gene054821:CDS
TTGGTTTGAATGAGACCTTC+AGG 0.357899 7.2:-27545436 None:intergenic
TTGTTGAATCGGCGAGGCTT+TGG 0.365934 7.2:+27545512 MS.gene054821:CDS
ATAATATTCCAAAACAAATA+GGG 0.370384 7.2:-27545546 None:intergenic
GGGAATTGCTTTAGCTCAAA+TGG 0.400122 7.2:+27546444 MS.gene054821:CDS
TTGGAGGGGTTTGTCTTGGT+TGG 0.400147 7.2:+27546495 MS.gene054821:CDS
AAAATAACTGAGGGATAGAT+TGG 0.402093 7.2:-27546246 None:intergenic
ATATTATCTATAAAACAAAT+TGG 0.403182 7.2:+27548471 MS.gene054821:CDS
GAATCAGAATATGAACAATA+AGG 0.403764 7.2:-27546351 None:intergenic
TTTCTCTTACCTGATAAAAC+TGG 0.406612 7.2:-27547800 None:intergenic
TTCTGTTGCTTCAATAGAAT+CGG 0.413602 7.2:-27545401 None:intergenic
GTTTCCAATGCACTTGGATC+AGG 0.417837 7.2:+27547581 MS.gene054821:CDS
AAATGTAGTCATTGTTGAAT+CGG 0.424749 7.2:+27545501 MS.gene054821:CDS
TTTATTAAAGTGCTTGGTTG+GGG 0.429230 7.2:+27546382 MS.gene054821:CDS
ATTTGGATTGGAATGATATG+TGG 0.431272 7.2:+27548419 MS.gene054821:CDS
TGGTGTCTCTCCCTTGGAAA+TGG 0.431654 7.2:-27545356 None:intergenic
AGCAATAGCTTATCCTCTGC+TGG 0.434351 7.2:+27545316 MS.gene054821:CDS
TGGGAAGGCATATTGTTCAT+AGG 0.440150 7.2:+27547433 MS.gene054821:CDS
AGTGAGCCAACGGCAATAAT+CGG 0.442245 7.2:-27546631 None:intergenic
CTGAACACTATTTAGAATCA+TGG 0.445752 7.2:-27547777 None:intergenic
TTGTTTCAGGGTATTTGGAT+TGG 0.453656 7.2:+27548407 MS.gene054821:intron
TTACAAGCACAAAGCAAAGT+TGG 0.454771 7.2:+27546301 MS.gene054821:CDS
AGGAGGAGGATGGCAGGCTT+TGG 0.455533 7.2:+27548107 MS.gene054821:CDS
AAGGTTGGAGGGGTTTGTCT+TGG 0.457416 7.2:+27546491 MS.gene054821:CDS
TACCTTATGGGACTCCCACT+TGG 0.462171 7.2:+27548156 MS.gene054821:CDS
GCTGATATAGCAGAGCTAGC+TGG 0.463457 7.2:+27546211 MS.gene054821:CDS
TTGGTTGGTGTAAAGAAGGT+TGG 0.465585 7.2:+27546476 MS.gene054821:CDS
CAGCTTATGATTTATCAGCT+TGG 0.466258 7.2:+27546422 MS.gene054821:CDS
GATTGTTCTCACTGGACACT+TGG 0.474558 7.2:+27546603 MS.gene054821:CDS
GGGAAGGCATATTGTTCATA+GGG 0.482782 7.2:+27547434 MS.gene054821:CDS
ACACCAATCTTAAAACTCCT+TGG 0.483912 7.2:+27546181 MS.gene054821:CDS
TGCTGTAGGAGGAGGATGGC+AGG 0.484254 7.2:+27548101 MS.gene054821:CDS
AAATGGTTTATGTTGTTGGT+TGG 0.485952 7.2:+27546461 MS.gene054821:CDS
AACCAAGTGGGAGTCCCATA+AGG 0.487565 7.2:-27548158 None:intergenic
TCCAGAATAGTCTCCAACAT+TGG 0.488066 7.2:-27545455 None:intergenic
ACGCTATGCGGACAAGCATA+TGG 0.495036 7.2:+27546061 MS.gene054821:CDS
GTTGTTGGTTGGTGTAAAGA+AGG 0.495105 7.2:+27546472 MS.gene054821:CDS
TAGGGGTCAATGCAGCTGTC+AGG 0.499403 7.2:+27547452 MS.gene054821:CDS
GGTCTCATTCAAACCAATGT+TGG 0.514964 7.2:+27545442 MS.gene054821:CDS
AATAAATTTGCAGCTTGGCA+TGG 0.517607 7.2:+27546024 MS.gene054821:intron
CGACAGTGTAGAAATGCAAA+AGG 0.518788 7.2:+27547721 MS.gene054821:CDS
GTGCTTGGTTGGGGTACAGC+TGG 0.521677 7.2:+27546391 MS.gene054821:CDS
ATGACCTTGATTGTTCTCAC+TGG 0.522516 7.2:+27546595 MS.gene054821:CDS
GATAAGTGGTGTCTCTCCCT+TGG 0.524347 7.2:-27545362 None:intergenic
TCTATCAGCATGAATGTGAA+TGG 0.539425 7.2:+27547409 MS.gene054821:intron
TGCGGACAAGCATATGGTGC+AGG 0.539879 7.2:+27546067 MS.gene054821:CDS
GCAATAGCTTATCCTCTGCT+GGG 0.541724 7.2:+27545317 MS.gene054821:CDS
GTAAGAGTTTCCAATGCACT+TGG 0.549510 7.2:+27547575 MS.gene054821:CDS
CTATATCAGCTTCTTGTCCA+AGG 0.554828 7.2:-27546198 None:intergenic
ATTGCAGGTGTTGCTGTAGG+AGG 0.556929 7.2:+27548090 MS.gene054821:intron
GAAAGAATGAAGCAATGGAC+TGG 0.558614 7.2:+27548653 MS.gene054821:CDS
AATGAAGCAATGGACTGGAC+AGG 0.559103 7.2:+27548658 MS.gene054821:CDS
GATAATCCGATTATTGCCGT+TGG 0.560602 7.2:+27546625 MS.gene054821:CDS
GTTGGTGTAAAGAAGGTTGG+AGG 0.564539 7.2:+27546479 MS.gene054821:CDS
ATCAAGTGTTTCCCCAGCAG+AGG 0.568521 7.2:-27545329 None:intergenic
TTTGGATTGGAATGATATGT+GGG 0.568808 7.2:+27548420 MS.gene054821:CDS
TTGGTGTAAAGAAGGTTGGA+GGG 0.573549 7.2:+27546480 MS.gene054821:CDS
GCTTATGATTTATCAGCTTG+GGG 0.573659 7.2:+27546424 MS.gene054821:CDS
GTGTCCAGTGAGAACAATCA+AGG 0.574439 7.2:-27546599 None:intergenic
ATTATTGCAGGTGTTGCTGT+AGG 0.577125 7.2:+27548087 MS.gene054821:intron
CATCAGAAAGAATGAAGCAA+TGG 0.577149 7.2:+27548648 MS.gene054821:CDS
CTATCAGCATGAATGTGAAT+GGG 0.578418 7.2:+27547410 MS.gene054821:intron
GAATGAGACCTTCAGGAGCA+TGG 0.586208 7.2:-27545429 None:intergenic
GCAGGTGTTGCTGTAGGAGG+AGG 0.586790 7.2:+27548093 MS.gene054821:intron
ACTTGATAGAACCATTTCCA+AGG 0.600184 7.2:+27545345 MS.gene054821:CDS
TGGTGTAAAGAAGGTTGGAG+GGG 0.608729 7.2:+27546481 MS.gene054821:CDS
GAACTTCACCATGCTCCTGA+AGG 0.613194 7.2:+27545421 MS.gene054821:CDS
CCATATTGAAAGTGAGCCAA+CGG 0.615566 7.2:-27546641 None:intergenic
ATGGCTAATGAAAATAACTG+AGG 0.619746 7.2:-27546256 None:intergenic
TAAAACAAATTGGAACAAAG+AGG 0.622527 7.2:+27548481 MS.gene054821:CDS
CTTGATAGAACCATTTCCAA+GGG 0.627194 7.2:+27545346 MS.gene054821:CDS
GTGTCCTGATCCAAGTGCAT+TGG 0.627327 7.2:-27547585 None:intergenic
GCAGGACAAGTAGATATGCT+CGG 0.628840 7.2:+27546085 MS.gene054821:CDS
CAATAGCTTATCCTCTGCTG+GGG 0.636892 7.2:+27545318 MS.gene054821:CDS
TGGCTAATGAAAATAACTGA+GGG 0.640051 7.2:-27546255 None:intergenic
TCAGCATGAATGTGAATGGG+TGG 0.646558 7.2:+27547413 MS.gene054821:intron
GGAGAGACACCACTTATCCA+AGG 0.652592 7.2:+27545367 MS.gene054821:CDS
CAGCATGAATGTGAATGGGT+GGG 0.654278 7.2:+27547414 MS.gene054821:intron
GTGTTGCTGTAGGAGGAGGA+TGG 0.654552 7.2:+27548097 MS.gene054821:CDS
TAGTCATTGTTGAATCGGCG+AGG 0.658192 7.2:+27545506 MS.gene054821:CDS
ATGAATGTGAATGGGTGGGA+AGG 0.661405 7.2:+27547418 MS.gene054821:CDS
CGGTGAAAATGACAGCAAAG+TGG 0.663149 7.2:-27547700 None:intergenic
GGAAGGCATATTGTTCATAG+GGG 0.737858 7.2:+27547435 MS.gene054821:CDS
GGTGTTGCATAAATGTACAG+TGG 0.747963 7.2:-27546163 None:intergenic
AGTGCACTAGAGACGCTATG+CGG 0.758720 7.2:+27546049 MS.gene054821:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAATAGAGATAAA+TGG + chr7.2:27544949-27544968 MS.gene054821:intron 10.0%
!! ATATTATCTATAAAACAAAT+TGG + chr7.2:27548471-27548490 MS.gene054821:CDS 10.0%
!!! ATTTTATTATTCTATAATGT+AGG - chr7.2:27545078-27545097 None:intergenic 10.0%
!!! CTAATAATTATAAAATTTTC+AGG + chr7.2:27548616-27548635 MS.gene054821:intron 10.0%
!!! TATTTTAACAAATATCTATA+TGG + chr7.2:27548290-27548309 MS.gene054821:intron 10.0%
!! ACTAATATAAAGTAGTTATT+AGG + chr7.2:27546906-27546925 MS.gene054821:intron 15.0%
!! ATAATATTCCAAAACAAATA+GGG - chr7.2:27545549-27545568 None:intergenic 15.0%
!! TCATTAATATTATGAATCAT+AGG - chr7.2:27546697-27546716 None:intergenic 15.0%
!!! AGTTATTAGGTTTTTATATT+TGG + chr7.2:27546919-27546938 MS.gene054821:intron 15.0%
!!! CTATATTAAACTTTTATGTA+AGG + chr7.2:27547028-27547047 MS.gene054821:intron 15.0%
!!! TTTTATAGATAATATACACT+AGG - chr7.2:27548466-27548485 None:intergenic 15.0%
!! AAAGTAACAATTTCATCAAA+AGG + chr7.2:27548534-27548553 MS.gene054821:intron 20.0%
!! ACAGTAATAACTGTATATAA+CGG + chr7.2:27544977-27544996 MS.gene054821:intron 20.0%
!! CATAATATTCCAAAACAAAT+AGG - chr7.2:27545550-27545569 None:intergenic 20.0%
!! TAAATGATTTCACTCTTATA+AGG + chr7.2:27545048-27545067 MS.gene054821:intron 20.0%
!! TATAATTATTAGACCTAAGA+GGG - chr7.2:27548608-27548627 None:intergenic 20.0%
!! TCATGCTACAATAATAATAT+TGG - chr7.2:27546876-27546895 None:intergenic 20.0%
!! TCCAACAAAAATATTTGTAA+AGG - chr7.2:27545590-27545609 None:intergenic 20.0%
!! TTATAATTATTAGACCTAAG+AGG - chr7.2:27548609-27548628 None:intergenic 20.0%
!!! AACCTTTTATATCTTCTATT+AGG - chr7.2:27547827-27547846 None:intergenic 20.0%
!!! AATATAACAAAGTGTCATTA+AGG - chr7.2:27548259-27548278 None:intergenic 20.0%
!!! AATATTGGTCAATTTTTAGA+GGG - chr7.2:27546861-27546880 None:intergenic 20.0%
!!! AATATTTTTGTTGGACATAT+TGG + chr7.2:27545595-27545614 MS.gene054821:CDS 20.0%
!!! AATTTATTGCACAACTTTTA+AGG - chr7.2:27546012-27546031 None:intergenic 20.0%
!!! ACCTTTACAAATATTTTTGT+TGG + chr7.2:27545586-27545605 MS.gene054821:CDS 20.0%
!!! ACCTTTTATATCTTCTATTA+GGG - chr7.2:27547826-27547845 None:intergenic 20.0%
!!! ATGAAACTTTTTTGTTTCTA+AGG - chr7.2:27545246-27545265 None:intergenic 20.0%
!!! CTATTTTGTTATTACCTTAT+GGG + chr7.2:27548144-27548163 MS.gene054821:CDS 20.0%
!!! CTCTTTTATGAAATTATTTC+AGG + chr7.2:27547063-27547082 MS.gene054821:intron 20.0%
!!! TAATATTGGTCAATTTTTAG+AGG - chr7.2:27546862-27546881 None:intergenic 20.0%
!!! TATTAAACTTTTATGTAAGG+TGG + chr7.2:27547031-27547050 MS.gene054821:intron 20.0%
!!! TCTATTTTGTTATTACCTTA+TGG + chr7.2:27548143-27548162 MS.gene054821:CDS 20.0%
!!! TTTCTTTTTATTAAAGTGCT+TGG + chr7.2:27546376-27546395 MS.gene054821:CDS 20.0%
!!! TTTTCAAAATGTGTAACAAA+CGG + chr7.2:27545902-27545921 MS.gene054821:intron 20.0%
! AAATGTAGTCATTGTTGAAT+CGG + chr7.2:27545501-27545520 MS.gene054821:CDS 25.0%
! AATAACTGTATATAACGGTT+TGG + chr7.2:27544982-27545001 MS.gene054821:intron 25.0%
! ACCCTAATAGAAGATATAAA+AGG + chr7.2:27547822-27547841 MS.gene054821:intron 25.0%
! AGAAGATATAAAAGGTTGTT+TGG + chr7.2:27547830-27547849 MS.gene054821:intron 25.0%
! ATTATTATTGTAGCATGAGT+TGG + chr7.2:27546878-27546897 MS.gene054821:intron 25.0%
! CGTTCAATTCTAATCTTATT+CGG + chr7.2:27546121-27546140 MS.gene054821:CDS 25.0%
! GAATCAGAATATGAACAATA+AGG - chr7.2:27546354-27546373 None:intergenic 25.0%
! GTCAAAAAATCTTACTGAAA+TGG - chr7.2:27547507-27547526 None:intergenic 25.0%
! TAAAACAAATTGGAACAAAG+AGG + chr7.2:27548481-27548500 MS.gene054821:CDS 25.0%
! TAAAATTTCACTCTTACTGA+TGG + chr7.2:27548379-27548398 MS.gene054821:intron 25.0%
! TCATAGAAAACTGCAAAAAT+AGG + chr7.2:27545262-27545281 MS.gene054821:intron 25.0%
! TGATATGATGCATTAAGAAA+CGG + chr7.2:27544916-27544935 MS.gene054821:intron 25.0%
! TTATTATTGTAGCATGAGTT+GGG + chr7.2:27546879-27546898 MS.gene054821:intron 25.0%
! TTTCAAAATTCTACATGCAT+TGG - chr7.2:27547238-27547257 None:intergenic 25.0%
!! AAATATTGTTTCAGGGTATT+TGG + chr7.2:27548402-27548421 MS.gene054821:intron 25.0%
!! AGGTTAGAATAGATTTGTTT+AGG - chr7.2:27546677-27546696 None:intergenic 25.0%
!! ATTTTATCGTTGCAACTATT+AGG - chr7.2:27547126-27547145 None:intergenic 25.0%
!! GTCTTTTGTAGCTAAAATAA+TGG - chr7.2:27547681-27547700 None:intergenic 25.0%
!! TGAAAAGAGTCTTATATCAA+GGG + chr7.2:27547253-27547272 MS.gene054821:intron 25.0%
!! TTGAAAAGAGTCTTATATCA+AGG + chr7.2:27547252-27547271 MS.gene054821:intron 25.0%
!!! AAAATAGGTATCGAAAATGA+CGG + chr7.2:27545277-27545296 MS.gene054821:intron 25.0%
!!! GTTTTTTACTTGGTTACAAA+GGG + chr7.2:27548178-27548197 MS.gene054821:CDS 25.0%
!!! TTTTATTAAAGTGCTTGGTT+GGG + chr7.2:27546381-27546400 MS.gene054821:CDS 25.0%
!!! TTTTTATTAAAGTGCTTGGT+TGG + chr7.2:27546380-27546399 MS.gene054821:CDS 25.0%
!!! TTTTTTACTTGGTTACAAAG+GGG + chr7.2:27548179-27548198 MS.gene054821:CDS 25.0%
AAAATAACTGAGGGATAGAT+TGG - chr7.2:27546249-27546268 None:intergenic 30.0%
AAATGGTTTATGTTGTTGGT+TGG + chr7.2:27546461-27546480 MS.gene054821:CDS 30.0%
AACCAAGTAAAAAACCAAGT+GGG - chr7.2:27548173-27548192 None:intergenic 30.0%
ACTGATGGAAATATTGTTTC+AGG + chr7.2:27548394-27548413 MS.gene054821:intron 30.0%
AGCTTATGATTTATCAGCTT+GGG + chr7.2:27546423-27546442 MS.gene054821:CDS 30.0%
ATGGCTAATGAAAATAACTG+AGG - chr7.2:27546259-27546278 None:intergenic 30.0%
CAAATACTAGTGAACTTTGT+CGG - chr7.2:27547204-27547223 None:intergenic 30.0%
CTGATGGAAATATTGTTTCA+GGG + chr7.2:27548395-27548414 MS.gene054821:intron 30.0%
CTTTCTCTGAACCTTATTAT+AGG + chr7.2:27547172-27547191 MS.gene054821:intron 30.0%
TAACCAAGTAAAAAACCAAG+TGG - chr7.2:27548174-27548193 None:intergenic 30.0%
TGGCTAATGAAAATAACTGA+GGG - chr7.2:27546258-27546277 None:intergenic 30.0%
TTACAAAAGCTACACTTTAG+AGG - chr7.2:27546799-27546818 None:intergenic 30.0%
TTAGAGGTTCAACAAAATCA+TGG - chr7.2:27546783-27546802 None:intergenic 30.0%
TTGTTATCATGTATCTGCAT+TGG + chr7.2:27545715-27545734 MS.gene054821:intron 30.0%
TTTCTCTTACCTGATAAAAC+TGG - chr7.2:27547803-27547822 None:intergenic 30.0%
! ATTTGGATTGGAATGATATG+TGG + chr7.2:27548419-27548438 MS.gene054821:CDS 30.0%
! CTGAACACTATTTAGAATCA+TGG - chr7.2:27547780-27547799 None:intergenic 30.0%
! GAAAGCTTTTGTATTATTGC+AGG + chr7.2:27548075-27548094 MS.gene054821:intron 30.0%
! TTCTGTTGCTTCAATAGAAT+CGG - chr7.2:27545404-27545423 None:intergenic 30.0%
! TTGACTTGACAAAATTATCG+CGG + chr7.2:27546755-27546774 MS.gene054821:intron 30.0%
! TTTATTAAAGTGCTTGGTTG+GGG + chr7.2:27546382-27546401 MS.gene054821:CDS 30.0%
! TTTGGATTGGAATGATATGT+GGG + chr7.2:27548420-27548439 MS.gene054821:CDS 30.0%
! TTTTTACTTGGTTACAAAGG+GGG + chr7.2:27548180-27548199 MS.gene054821:CDS 30.0%
! TTTTTGTGCTGGAAAATTGA+TGG - chr7.2:27546278-27546297 None:intergenic 30.0%
!! AGCTATTATGCTTTGTTTAG+AGG + chr7.2:27546561-27546580 MS.gene054821:CDS 30.0%
!! GTTGTTTCAAACTTCTCTTT+TGG + chr7.2:27545655-27545674 MS.gene054821:CDS 30.0%
!! TTATGCTTTGTTTAGAGGTT+TGG + chr7.2:27546566-27546585 MS.gene054821:CDS 30.0%
!!! AAACTTCTCTTTTGGTTTCA+TGG + chr7.2:27545663-27545682 MS.gene054821:CDS 30.0%
!!! CTTTTTTGTTTCTAAGGTGT+TGG - chr7.2:27545240-27545259 None:intergenic 30.0%
!!! GGTTTTTTACTTGGTTACAA+AGG + chr7.2:27548177-27548196 MS.gene054821:CDS 30.0%
AATAAATTTGCAGCTTGGCA+TGG + chr7.2:27546024-27546043 MS.gene054821:intron 35.0%
ACACCAATCTTAAAACTCCT+TGG + chr7.2:27546181-27546200 MS.gene054821:CDS 35.0%
ACTTGATAGAACCATTTCCA+AGG + chr7.2:27545345-27545364 MS.gene054821:CDS 35.0%
ATGCATTAAGAAACGGTGTT+TGG + chr7.2:27544923-27544942 MS.gene054821:intron 35.0%
ATTCACATTCATGCTGATAG+AGG - chr7.2:27547410-27547429 None:intergenic 35.0%
CAGCTTATGATTTATCAGCT+TGG + chr7.2:27546422-27546441 MS.gene054821:CDS 35.0%
CATCAGAAAGAATGAAGCAA+TGG + chr7.2:27548648-27548667 MS.gene054821:CDS 35.0%
CTATCAGCATGAATGTGAAT+GGG + chr7.2:27547410-27547429 MS.gene054821:intron 35.0%
CTTGATAGAACCATTTCCAA+GGG + chr7.2:27545346-27545365 MS.gene054821:CDS 35.0%
GATGAGTGTAAGGTGTATAT+TGG - chr7.2:27546965-27546984 None:intergenic 35.0%
GCTCAAATGGTTTATGTTGT+TGG + chr7.2:27546457-27546476 MS.gene054821:CDS 35.0%
GCTTATGATTTATCAGCTTG+GGG + chr7.2:27546424-27546443 MS.gene054821:CDS 35.0%
GTTGTTGAATCTCTCATCAT+TGG + chr7.2:27547641-27547660 MS.gene054821:CDS 35.0%
TAGTATCAGAACTAAGTCCT+AGG - chr7.2:27547011-27547030 None:intergenic 35.0%
TATTGAATCTTCTCCCTCTT+AGG + chr7.2:27548592-27548611 MS.gene054821:intron 35.0%
TCTAAGTTAGCATCTCTTCT+TGG + chr7.2:27547749-27547768 MS.gene054821:CDS 35.0%
TCTATCAGCATGAATGTGAA+TGG + chr7.2:27547409-27547428 MS.gene054821:intron 35.0%
TGTGCAATAAATTTGCAGCT+TGG + chr7.2:27546019-27546038 MS.gene054821:intron 35.0%
TTACAAGCACAAAGCAAAGT+TGG + chr7.2:27546301-27546320 MS.gene054821:CDS 35.0%
TTCACATTCATGCTGATAGA+GGG - chr7.2:27547409-27547428 None:intergenic 35.0%
! AAAATGACGGAAACATTGCA+GGG + chr7.2:27545290-27545309 MS.gene054821:intron 35.0%
! AACACTTTTCCTTGGATAAG+TGG - chr7.2:27545379-27545398 None:intergenic 35.0%
! ACAAAGCAAAGTTGGAGTTT+TGG + chr7.2:27546309-27546328 MS.gene054821:CDS 35.0%
! ACTGAATATTTTGCTGCTCT+TGG - chr7.2:27547611-27547630 None:intergenic 35.0%
! ATTGCAAGCCCTATTTGTTT+TGG + chr7.2:27545538-27545557 MS.gene054821:CDS 35.0%
! CTGAATATTTTGCTGCTCTT+GGG - chr7.2:27547610-27547629 None:intergenic 35.0%
! CTTTTGCATTTCTACACTGT+CGG - chr7.2:27547723-27547742 None:intergenic 35.0%
! GACTTGGTCATAAGTTTCAA+TGG + chr7.2:27545023-27545042 MS.gene054821:intron 35.0%
! GCTTAAGTTGCTTGAAAATG+AGG + chr7.2:27545986-27546005 MS.gene054821:intron 35.0%
! GCTTGTAAGAATTTTTGTGC+TGG - chr7.2:27546289-27546308 None:intergenic 35.0%
! TTGTTTCAGGGTATTTGGAT+TGG + chr7.2:27548407-27548426 MS.gene054821:intron 35.0%
!! TTGGCTTTTAAGGATCTTTG+GGG + chr7.2:27546514-27546533 MS.gene054821:CDS 35.0%
!!! ATTTTTAGAGGGAGCATTCT+TGG - chr7.2:27546850-27546869 None:intergenic 35.0%
!!! GTTGGCTTTTAAGGATCTTT+GGG + chr7.2:27546513-27546532 MS.gene054821:CDS 35.0%
!!! TGTCCAAGGAGTTTTAAGAT+TGG - chr7.2:27546187-27546206 None:intergenic 35.0%
!!! TTTGTCTTGGTTGGCTTTTA+AGG + chr7.2:27546504-27546523 MS.gene054821:CDS 35.0%
AAACCCTAGTGATGAGTGTA+AGG - chr7.2:27546975-27546994 None:intergenic 40.0%
ACACCTTACACTCATCACTA+GGG + chr7.2:27546969-27546988 MS.gene054821:intron 40.0%
ACCAATGTTGGAGACTATTC+TGG + chr7.2:27545454-27545473 MS.gene054821:CDS 40.0%
ATACTAGTGAACTTTGTCGG+AGG - chr7.2:27547201-27547220 None:intergenic 40.0%
ATGACCTTGATTGTTCTCAC+TGG + chr7.2:27546595-27546614 MS.gene054821:CDS 40.0%
CCATATTGAAAGTGAGCCAA+CGG - chr7.2:27546644-27546663 None:intergenic 40.0%
CGACAGTGTAGAAATGCAAA+AGG + chr7.2:27547721-27547740 MS.gene054821:CDS 40.0%
CTATATCAGCTTCTTGTCCA+AGG - chr7.2:27546201-27546220 None:intergenic 40.0%
GAAAGAATGAAGCAATGGAC+TGG + chr7.2:27548653-27548672 MS.gene054821:CDS 40.0%
GATAATCCGATTATTGCCGT+TGG + chr7.2:27546625-27546644 MS.gene054821:CDS 40.0%
GGTCTCATTCAAACCAATGT+TGG + chr7.2:27545442-27545461 MS.gene054821:CDS 40.0%
GTAAGAGTTTCCAATGCACT+TGG + chr7.2:27547575-27547594 MS.gene054821:CDS 40.0%
TACACCTTACACTCATCACT+AGG + chr7.2:27546968-27546987 MS.gene054821:intron 40.0%
TACTAGTGAACTTTGTCGGA+GGG - chr7.2:27547200-27547219 None:intergenic 40.0%
TAGAGAGTAGTATGAGACAG+TGG + chr7.2:27547313-27547332 MS.gene054821:intron 40.0%
TCCAGAATAGTCTCCAACAT+TGG - chr7.2:27545458-27545477 None:intergenic 40.0%
TTGGTGTAAAGAAGGTTGGA+GGG + chr7.2:27546480-27546499 MS.gene054821:CDS 40.0%
! AAATGACGGAAACATTGCAG+GGG + chr7.2:27545291-27545310 MS.gene054821:intron 40.0%
! AATCGGAGAACACTTTTCCT+TGG - chr7.2:27545387-27545406 None:intergenic 40.0%
! AGTTTGTAATGGAGGAGACT+TGG + chr7.2:27545007-27545026 MS.gene054821:intron 40.0%
! GAAAATGACGGAAACATTGC+AGG + chr7.2:27545289-27545308 MS.gene054821:intron 40.0%
! GGAAGGCATATTGTTCATAG+GGG + chr7.2:27547435-27547454 MS.gene054821:CDS 40.0%
! GGGAAGGCATATTGTTCATA+GGG + chr7.2:27547434-27547453 MS.gene054821:CDS 40.0%
! GGGAATTGCTTTAGCTCAAA+TGG + chr7.2:27546444-27546463 MS.gene054821:CDS 40.0%
! GGTGTTGCATAAATGTACAG+TGG - chr7.2:27546166-27546185 None:intergenic 40.0%
! GTTGTTGGTTGGTGTAAAGA+AGG + chr7.2:27546472-27546491 MS.gene054821:CDS 40.0%
! GTTTGGTGAGTTTGTAATGG+AGG + chr7.2:27544999-27545018 MS.gene054821:intron 40.0%
! TGGGAAGGCATATTGTTCAT+AGG + chr7.2:27547433-27547452 MS.gene054821:CDS 40.0%
! TTGGTTGGTGTAAAGAAGGT+TGG + chr7.2:27546476-27546495 MS.gene054821:CDS 40.0%
! TTGGTTTGAATGAGACCTTC+AGG - chr7.2:27545439-27545458 None:intergenic 40.0%
!! ACGGTTTGGTGAGTTTGTAA+TGG + chr7.2:27544996-27545015 MS.gene054821:intron 40.0%
!! AGTGTTCAGCCAGTTTTATC+AGG + chr7.2:27547791-27547810 MS.gene054821:CDS 40.0%
!! ATTATTGCAGGTGTTGCTGT+AGG + chr7.2:27548087-27548106 MS.gene054821:intron 40.0%
!!! CTCCCACTTGGTTTTTTACT+TGG + chr7.2:27548168-27548187 MS.gene054821:CDS 40.0%
!!! GGTTGGCTTTTAAGGATCTT+TGG + chr7.2:27546512-27546531 MS.gene054821:CDS 40.0%
ACTAGTGAACTTTGTCGGAG+GGG - chr7.2:27547199-27547218 None:intergenic 45.0%
AGCAATAGCTTATCCTCTGC+TGG + chr7.2:27545316-27545335 MS.gene054821:CDS 45.0%
AGTGAGCCAACGGCAATAAT+CGG - chr7.2:27546634-27546653 None:intergenic 45.0%
ATGAATGTGAATGGGTGGGA+AGG + chr7.2:27547418-27547437 MS.gene054821:CDS 45.0%
CAACCGTGTTTCCAAACCTA+TGG - chr7.2:27546738-27546757 None:intergenic 45.0%
CAATAGCTTATCCTCTGCTG+GGG + chr7.2:27545318-27545337 MS.gene054821:CDS 45.0%
CAGCATGAATGTGAATGGGT+GGG + chr7.2:27547414-27547433 MS.gene054821:intron 45.0%
CGAATTTGACAAACTCGAGG+TGG - chr7.2:27546827-27546846 None:intergenic 45.0%
CGGTGAAAATGACAGCAAAG+TGG - chr7.2:27547703-27547722 None:intergenic 45.0%
CTAAGTCCTAGGCGTGTATT+AGG - chr7.2:27547000-27547019 None:intergenic 45.0%
GATTGTTCTCACTGGACACT+TGG + chr7.2:27546603-27546622 MS.gene054821:CDS 45.0%
GCAATAGCTTATCCTCTGCT+GGG + chr7.2:27545317-27545336 MS.gene054821:CDS 45.0%
GCACACGCTATTGTGCAATA+AGG + chr7.2:27548045-27548064 MS.gene054821:intron 45.0%
GCAGGACAAGTAGATATGCT+CGG + chr7.2:27546085-27546104 MS.gene054821:CDS 45.0%
GGTGAGACATGATTGAGTAG+TGG + chr7.2:27548311-27548330 MS.gene054821:intron 45.0%
GTGTCCAGTGAGAACAATCA+AGG - chr7.2:27546602-27546621 None:intergenic 45.0%
GTTGGTGTAAAGAAGGTTGG+AGG + chr7.2:27546479-27546498 MS.gene054821:CDS 45.0%
GTTTCCAATGCACTTGGATC+AGG + chr7.2:27547581-27547600 MS.gene054821:CDS 45.0%
GTTTCTCCTAATACACGCCT+AGG + chr7.2:27546991-27547010 MS.gene054821:intron 45.0%
TAGTCATTGTTGAATCGGCG+AGG + chr7.2:27545506-27545525 MS.gene054821:CDS 45.0%
TCAGCATGAATGTGAATGGG+TGG + chr7.2:27547413-27547432 MS.gene054821:intron 45.0%
TCGCCATAGGTTTGGAAACA+CGG + chr7.2:27546732-27546751 MS.gene054821:intron 45.0%
TGGCGAATTTGACAAACTCG+AGG - chr7.2:27546830-27546849 None:intergenic 45.0%
TGGTGTAAAGAAGGTTGGAG+GGG + chr7.2:27546481-27546500 MS.gene054821:CDS 45.0%
TTGTACTCTCGCCATAGGTT+TGG + chr7.2:27546724-27546743 MS.gene054821:intron 45.0%
! AATGACGGAAACATTGCAGG+GGG + chr7.2:27545292-27545311 MS.gene054821:intron 45.0%
!! AATGAAGCAATGGACTGGAC+AGG + chr7.2:27548658-27548677 MS.gene054821:CDS 45.0%
!! AGTTGGAGTTTTGGCATGGT+TGG + chr7.2:27546318-27546337 MS.gene054821:CDS 45.0%
!! CCGTTGGCTCACTTTCAATA+TGG + chr7.2:27546641-27546660 MS.gene054821:CDS 45.0%
!! GCAAAGTTGGAGTTTTGGCA+TGG + chr7.2:27546314-27546333 MS.gene054821:CDS 45.0%
!! GTTGGAGTTTTGGCATGGTT+GGG + chr7.2:27546319-27546338 MS.gene054821:CDS 45.0%
!! ATTCAATTTAAATTAAATAA+AGG - chr7.2:27545746-27545765 None:intergenic 5.0%
AACCAAGTGGGAGTCCCATA+AGG - chr7.2:27548161-27548180 None:intergenic 50.0%
AAGGTTGGAGGGGTTTGTCT+TGG + chr7.2:27546491-27546510 MS.gene054821:CDS 50.0%
ACGCTATGCGGACAAGCATA+TGG + chr7.2:27546061-27546080 MS.gene054821:CDS 50.0%
AGTGCACTAGAGACGCTATG+CGG + chr7.2:27546049-27546068 MS.gene054821:CDS 50.0%
ATCAAGTGTTTCCCCAGCAG+AGG - chr7.2:27545332-27545351 None:intergenic 50.0%
CAAAGGGGGATACAGAGTAC+AGG + chr7.2:27548194-27548213 MS.gene054821:CDS 50.0%
CTCGATTGTACTCTCGCCAT+AGG + chr7.2:27546719-27546738 MS.gene054821:intron 50.0%
GAACTTCACCATGCTCCTGA+AGG + chr7.2:27545421-27545440 MS.gene054821:CDS 50.0%
GAATGAGACCTTCAGGAGCA+TGG - chr7.2:27545432-27545451 None:intergenic 50.0%
GATAAGTGGTGTCTCTCCCT+TGG - chr7.2:27545365-27545384 None:intergenic 50.0%
GCTGATATAGCAGAGCTAGC+TGG + chr7.2:27546211-27546230 MS.gene054821:CDS 50.0%
GGAGAGACACCACTTATCCA+AGG + chr7.2:27545367-27545386 MS.gene054821:CDS 50.0%
GTCGGAGGGGTCCTATAATA+AGG - chr7.2:27547186-27547205 None:intergenic 50.0%
GTGTCCTGATCCAAGTGCAT+TGG - chr7.2:27547588-27547607 None:intergenic 50.0%
TACCTTATGGGACTCCCACT+TGG + chr7.2:27548156-27548175 MS.gene054821:CDS 50.0%
TGGTGTCTCTCCCTTGGAAA+TGG - chr7.2:27545359-27545378 None:intergenic 50.0%
TGTTGAATCGGCGAGGCTTT+GGG + chr7.2:27545513-27545532 MS.gene054821:CDS 50.0%
TTGGAGGGGTTTGTCTTGGT+TGG + chr7.2:27546495-27546514 MS.gene054821:CDS 50.0%
TTGTTGAATCGGCGAGGCTT+TGG + chr7.2:27545512-27545531 MS.gene054821:CDS 50.0%
!! ATTGCAGGTGTTGCTGTAGG+AGG + chr7.2:27548090-27548109 MS.gene054821:intron 50.0%
GCGCGCACACACATACATGA+GGG - chr7.2:27544893-27544912 None:intergenic 55.0%
TAGGGGTCAATGCAGCTGTC+AGG + chr7.2:27547452-27547471 MS.gene054821:CDS 55.0%
TGCGGACAAGCATATGGTGC+AGG + chr7.2:27546067-27546086 MS.gene054821:CDS 55.0%
! GTGTTGCTGTAGGAGGAGGA+TGG + chr7.2:27548097-27548116 MS.gene054821:CDS 55.0%
AGGAGGAGGATGGCAGGCTT+TGG + chr7.2:27548107-27548126 MS.gene054821:CDS 60.0%
CGCGCGCACACACATACATG+AGG - chr7.2:27544894-27544913 None:intergenic 60.0%
GTGCTTGGTTGGGGTACAGC+TGG + chr7.2:27546391-27546410 MS.gene054821:CDS 60.0%
TGCTGTAGGAGGAGGATGGC+AGG + chr7.2:27548101-27548120 MS.gene054821:CDS 60.0%
!! GCAGGTGTTGCTGTAGGAGG+AGG + chr7.2:27548093-27548112 MS.gene054821:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 27544869 27548703 27544869 ID=MS.gene054821
chr7.2 mRNA 27544869 27548703 27544869 ID=MS.gene054821.t1;Parent=MS.gene054821
chr7.2 exon 27544869 27544896 27544869 ID=MS.gene054821.t1.exon1;Parent=MS.gene054821.t1
chr7.2 CDS 27544869 27544896 27544869 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
chr7.2 exon 27545311 27545684 27545311 ID=MS.gene054821.t1.exon2;Parent=MS.gene054821.t1
chr7.2 CDS 27545311 27545684 27545311 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
chr7.2 exon 27546037 27546662 27546037 ID=MS.gene054821.t1.exon3;Parent=MS.gene054821.t1
chr7.2 CDS 27546037 27546662 27546037 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
chr7.2 exon 27547417 27547473 27547417 ID=MS.gene054821.t1.exon4;Parent=MS.gene054821.t1
chr7.2 CDS 27547417 27547473 27547417 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
chr7.2 exon 27547574 27547812 27547574 ID=MS.gene054821.t1.exon5;Parent=MS.gene054821.t1
chr7.2 CDS 27547574 27547812 27547574 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
chr7.2 exon 27548097 27548215 27548097 ID=MS.gene054821.t1.exon6;Parent=MS.gene054821.t1
chr7.2 CDS 27548097 27548215 27548097 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
chr7.2 exon 27548416 27548502 27548416 ID=MS.gene054821.t1.exon7;Parent=MS.gene054821.t1
chr7.2 CDS 27548416 27548502 27548416 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
chr7.2 exon 27548638 27548703 27548638 ID=MS.gene054821.t1.exon8;Parent=MS.gene054821.t1
chr7.2 CDS 27548638 27548703 27548638 ID=cds.MS.gene054821.t1;Parent=MS.gene054821.t1
Gene Sequence

>MS.gene054821

ATGTTCTTCGACTACGTTAATCCCTCATGTATGTGTGTGCGCGCGTGTGATATGATGCATTAAGAAACGGTGTTTGGATAAAATAAAAAATAGAGATAAATGGATTTAACAGTAATAACTGTATATAACGGTTTGGTGAGTTTGTAATGGAGGAGACTTGGTCATAAGTTTCAATGGCTTAAATGATTTCACTCTTATAAGGCTATCCTACATTATAGAATAATAAAATTCTTCATCTTTTCTCTCACTAAAGAAACAATGATTGAAACTAATTCATAGAAGCAAATGCACATAAAAAAATCTTTTGTTATTCTTCTTTCTTTTGCATGAGTTTTTAACTTTTGCTTGCAAATGCTTCATTTCATTCTCCAACACCTTAGAAACAAAAAAGTTTCATAGAAAACTGCAAAAATAGGTATCGAAAATGACGGAAACATTGCAGGGGGAAGCAATAGCTTATCCTCTGCTGGGGAAACACTTGATAGAACCATTTCCAAGGGAGAGACACCACTTATCCAAGGAAAAGTGTTCTCCGATTCTATTGAAGCAACAGAACTTCACCATGCTCCTGAAGGTCTCATTCAAACCAATGTTGGAGACTATTCTGGAATAAATAGCTTTGAAGATTTAACAAATGTAGTCATTGTTGAATCGGCGAGGCTTTGGGCGATTGCAAGCCCTATTTGTTTTGGAATATTATGCAATTATGCAATCAATACCTTTACAAATATTTTTGTTGGACATATTGGAGATTTAGAACTTACATCAGTTTCAATCTCTTTGTCTGTTGTTTCAAACTTCTCTTTTGGTTTCATGGTTAGTAATTCTATTATCTTTTTGATTCATTTGTTATCATGTATCTGCATTGGATGCTCCTTTATTTAATTTAAATTGAATTTTGATTTACGAAAACAAAAAGCGATTGTTATATTCAATAATTTAGACATTGATTTTTAGAGATTCTTAAAATAAAAGAAGAAAAGCTATCAGAAAATGATTATTAAAAGAAGCTACTCAAAACATCGTCTCACTTTTTTCAAAATGTGTAACAAACGGATGAAAACTTTTAAAAATGATTTTTTGCTCAAGTTTGAAAGAAAAAAAATTAGCGTTTTTTGCTTAAGTTGCTTGAAAATGAGGCCTTAAAAGTTGTGCAATAAATTTGCAGCTTGGCATGGCAAGTGCACTAGAGACGCTATGCGGACAAGCATATGGTGCAGGACAAGTAGATATGCTCGGTGTTTACATGCAACGTTCAATTCTAATCTTATTCGGTTCATGTTTTCTCATCTTACCACTGTACATTTATGCAACACCAATCTTAAAACTCCTTGGACAAGAAGCTGATATAGCAGAGCTAGCTGGAGTATTCACAGTCCAATCTATCCCTCAGTTATTTTCATTAGCCATCAATTTTCCAGCACAAAAATTCTTACAAGCACAAAGCAAAGTTGGAGTTTTGGCATGGTTGGGTTTCATATTCCTTATTGTTCATATTCTGATTCTGTTTCTTTTTATTAAAGTGCTTGGTTGGGGTACAGCTGGTGCTGCTGCAGCTTATGATTTATCAGCTTGGGGAATTGCTTTAGCTCAAATGGTTTATGTTGTTGGTTGGTGTAAAGAAGGTTGGAGGGGTTTGTCTTGGTTGGCTTTTAAGGATCTTTGGGGTTTTACTAAATTATCTTTTGCTTCAGCTATTATGCTTTGTTTAGAGGTTTGGTATTTTATGACCTTGATTGTTCTCACTGGACACTTGGATAATCCGATTATTGCCGTTGGCTCACTTTCAATATGGTAAGATCACACCTAAACAAATCTATTCTAACCTATGATTCATAATATTAATGATACTCGATTGTACTCTCGCCATAGGTTTGGAAACACGGTTGACTTGACAAAATTATCGCGGCACCATGATTTTGTTGAACCTCTAAAGTGTAGCTTTTGTAAAATCACCACCTCGAGTTTGTCAAATTCGCCAAGAATGCTCCCTCTAAAAATTGACCAATATTATTATTGTAGCATGAGTTGGGTGTAACTAATATAAAGTAGTTATTAGGTTTTTATATTTGGCATAACTCGCTTCAATATTTCCAATATACACCTTACACTCATCACTAGGGTTTCTCCTAATACACGCCTAGGACTTAGTTCTGATACTATATTAAACTTTTATGTAAGGTGGTGAATGTCACTCTTTTATGAAATTATTTCAGGTCTTATCTGTATTCAATATTGTGCGACTTGAATTTTTCCTAATAGTTGCAACGATAAAATATTCTAAACATGACACATAAATCATACTTTCTCTGAACCTTATTATAGGACCCCTCCGACAAAGTTCACTAGTATTTGTAAAGAAATTACCAATGCATGTAGAATTTTGAAAAGAGTCTTATATCAAGGGAAAAAGAAAAAATGAAAATATATTTTATAATTAAGTATAGAGAGTAGTATGAGACAGTGGCTTCTTGCTTTTCACACAAACTTCATGTTTTCTCTTATATACTCTTAACTTACTAATATAGTTTTGTTTTCCCTCTATCAGCATGAATGTGAATGGGTGGGAAGGCATATTGTTCATAGGGGTCAATGCAGCTGTCAGGTAAACTCTTGATTTGTGATTATTGTTATTCCATTTCAGTAAGATTTTTTGACAACTTCTATGAATAATTTTTGTTTATGTTTTGTTTTACTTTTATCAGTGTAAGAGTTTCCAATGCACTTGGATCAGGACACCCAAGAGCAGCAAAATATTCAGTCATTGTCACAGTTGTTGAATCTCTCATCATTGGAATAGTGTCTGCAGCCATTATTTTAGCTACAAAAGACCACTTTGCTGTCATTTTCACCGACAGTGTAGAAATGCAAAAGGCAGTTTCTAAGTTAGCATCTCTTCTTGGCATAACCATGATTCTAAATAGTGTTCAGCCAGTTTTATCAGGTAAGAGAAACCCTAATAGAAGATATAAAAGGTTGTTTGGATTGACTCGTTTTAGTTTATCTTGCGAGACTATTTCGAATAGCTTATGAAAACGACTCATGAAGTGTCTATAACTTGTTTTCAGCTTATGAAAATAGCTTATAACTTGTATGAAAATTGTTTGACTTTATCAAATCTTTGTTATAGAAATAGCTTATACATAAGCAGTTATATGATAAATGCTTATGTGCGTGCACACGCTATTGTGCAATAAGGAAATCATGAAAGCTTTTGTATTATTGCAGGTGTTGCTGTAGGAGGAGGATGGCAGGCTTTGGTAGCTTACATTAATCTATTTTGTTATTACCTTATGGGACTCCCACTTGGTTTTTTACTTGGTTACAAAGGGGGATACAGAGTACAGGTAATGTACTTACATGACGATCATTCAAACATATATCGTACCTTAATGACACTTTGTTATATTATTTTCTAAGATATTTTAACAAATATCTATATGGTGAGACATGATTGAGTAGTGGAGTATAATTTGCTACACTGTCGATGCATGTCACTAAACTCGATTTTAAAATTTCACTCTTACTGATGGAAATATTGTTTCAGGGTATTTGGATTGGAATGATATGTGGGACAGTTTTGCAGACAGCTATCCTAGTGTATATTATCTATAAAACAAATTGGAACAAAGAGGTGAGATTAAGTGCTTTTCTTATTAATCATCAAAGTAACAATTTCATCAAAAGGCTATGATGACAAAAGCTTAAGAAAGTGAGAATATATATTGAATCTTCTCCCTCTTAGGTCTAATAATTATAAAATTTTCAGGTTGAACAAGCATCAGAAAGAATGAAGCAATGGACTGGACAGGAATTAGAACTGAGTGACATGTAG
Protein sequence

>MS.gene054821.t1

MFFDYVNPSWGSNSLSSAGETLDRTISKGETPLIQGKVFSDSIEATELHHAPEGLIQTNVGDYSGINSFEDLTNVVIVESARLWAIASPICFGILCNYAINTFTNIFVGHIGDLELTSVSISLSVVSNFSFGFMLGMASALETLCGQAYGAGQVDMLGVYMQRSILILFGSCFLILPLYIYATPILKLLGQEADIAELAGVFTVQSIPQLFSLAINFPAQKFLQAQSKVGVLAWLGFIFLIVHILILFLFIKVLGWGTAGAAAAYDLSAWGIALAQMVYVVGWCKEGWRGLSWLAFKDLWGFTKLSFASAIMLCLEVWYFMTLIVLTGHLDNPIIAVGSLSICMNVNGWEGILFIGVNAAVSVRVSNALGSGHPRAAKYSVIVTVVESLIIGIVSAAIILATKDHFAVIFTDSVEMQKAVSKLASLLGITMILNSVQPVLSGVAVGGGWQALVAYINLFCYYLMGLPLGFLLGYKGGYRVQGIWIGMICGTVLQTAILVYIIYKTNWNKEVEQASERMKQWTGQELELSDM