AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042538

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042538.t1 MTR_7g087410 96.545 492 15 1 1 490 1 492 0.0 952
MS.gene042538.t1 MTR_7g087410 97.519 403 10 0 88 490 1 403 0.0 793
MS.gene042538.t1 MTR_7g087400 71.074 484 138 2 2 485 3 484 0.0 721
MS.gene042538.t1 MTR_6g027200 66.667 492 158 4 2 488 6 496 0.0 653
MS.gene042538.t1 MTR_7g087370 64.959 488 140 3 2 489 3 459 0.0 639
MS.gene042538.t1 MTR_7g087430 79.798 396 79 1 88 483 1 395 0.0 637
MS.gene042538.t1 MTR_6g027190 66.737 475 149 3 1 474 3 469 0.0 603
MS.gene042538.t1 MTR_5g032720 49.351 462 231 2 22 482 29 488 4.18e-167 481
MS.gene042538.t1 MTR_3g099700 47.746 488 247 2 2 481 12 499 3.40e-162 469
MS.gene042538.t1 MTR_5g032750 46.875 480 251 4 4 481 12 489 4.90e-160 463
MS.gene042538.t1 MTR_1g021740 46.895 467 248 0 15 481 21 487 7.24e-151 439
MS.gene042538.t1 MTR_8g069470 46.239 452 243 0 22 473 43 494 1.23e-150 440
MS.gene042538.t1 MTR_3g099720 46.444 478 248 2 9 482 17 490 1.33e-147 431
MS.gene042538.t1 MTR_1g100180 45.336 461 248 2 30 490 44 500 7.40e-146 427
MS.gene042538.t1 MTR_5g033100 45.652 460 250 0 30 489 57 516 8.21e-144 422
MS.gene042538.t1 MTR_4g048000 45.833 456 242 2 25 475 56 511 1.87e-138 409
MS.gene042538.t1 MTR_2g088400 45.815 454 245 1 23 475 46 499 6.37e-136 402
MS.gene042538.t1 MTR_2g022980 42.888 464 260 2 17 475 32 495 1.72e-132 393
MS.gene042538.t1 MTR_6g047610 39.413 477 281 4 5 475 1 475 3.87e-122 366
MS.gene042538.t1 MTR_6g047640 40.042 477 279 2 5 475 1 476 9.60e-122 365
MS.gene042538.t1 MTR_2g088400 43.478 437 233 2 11 433 21 457 1.03e-116 351
MS.gene042538.t1 MTR_6g047640 37.736 477 271 3 5 475 1 457 1.74e-114 345
MS.gene042538.t1 MTR_5g090290 41.116 484 275 3 4 481 20 499 1.10e-112 345
MS.gene042538.t1 MTR_6g047590 38.526 475 284 4 5 473 1 473 1.14e-109 333
MS.gene042538.t1 MTR_8g063590 36.534 479 289 5 6 471 21 497 2.95e-96 300
MS.gene042538.t1 MTR_6g081410 35.625 480 300 5 4 478 2 477 5.33e-93 291
MS.gene042538.t1 MTR_6g081780 35.477 482 299 6 4 480 2 476 1.37e-90 285
MS.gene042538.t1 MTR_7g082810 37.473 459 284 2 29 484 20 478 9.35e-89 280
MS.gene042538.t1 MTR_1g108810 34.335 466 301 2 5 465 6 471 7.29e-88 278
MS.gene042538.t1 MTR_6g066380 35.729 473 295 5 4 470 2 471 2.70e-86 273
MS.gene042538.t1 MTR_6g081300 34.746 472 299 7 21 487 1 468 3.78e-86 273
MS.gene042538.t1 MTR_5g087930 37.731 432 245 5 54 482 51 461 6.45e-85 270
MS.gene042538.t1 MTR_6g004220 33.755 474 302 4 1 470 1 466 5.06e-83 265
MS.gene042538.t1 MTR_8g106670 33.836 464 293 5 13 472 3 456 4.41e-82 263
MS.gene042538.t1 MTR_6g081810 35.319 470 266 8 3 470 10 443 6.39e-81 259
MS.gene042538.t1 MTR_6g004220 33.619 467 298 4 1 463 8 466 1.14e-79 257
MS.gene042538.t1 MTR_6g081240 33.871 434 283 3 32 463 14 445 1.39e-79 255
MS.gene042538.t1 MTR_6g081390 33.769 459 296 6 25 478 8 463 1.71e-79 255
MS.gene042538.t1 MTR_6g081400 32.743 452 298 4 25 472 11 460 1.36e-78 253
MS.gene042538.t1 MTR_1g108840 33.054 478 310 5 1 470 8 483 1.69e-76 248
MS.gene042538.t1 MTR_2g079020 33.478 460 297 3 10 461 12 470 1.99e-76 248
MS.gene042538.t1 MTR_2g078930 34.498 458 287 4 12 461 24 476 2.08e-76 248
MS.gene042538.t1 MTR_2g078960 35.484 434 279 1 29 461 33 466 2.97e-74 242
MS.gene042538.t1 MTR_1g109060 33.405 467 309 2 5 470 1 466 9.85e-73 238
MS.gene042538.t1 MTR_1g108990 32.623 469 311 3 5 470 1 467 1.88e-72 237
MS.gene042538.t1 MTR_2g078970 34.437 453 288 3 17 461 13 464 3.53e-72 237
MS.gene042538.t1 MTR_6g081310 33.921 454 278 5 51 485 18 468 2.43e-71 234
MS.gene042538.t1 MTR_1g109060 33.405 467 308 3 5 470 1 465 1.63e-70 233
MS.gene042538.t1 MTR_5g090280 34.977 426 234 8 88 481 1 415 7.45e-68 224
MS.gene042538.t1 MTR_7g082800 33.594 384 254 1 88 470 1 384 8.95e-67 220
MS.gene042538.t1 MTR_3g111020 27.823 496 317 9 16 487 35 513 1.64e-54 192
MS.gene042538.t1 MTR_6g004220 31.156 398 262 4 1 394 8 397 2.33e-54 189
MS.gene042538.t1 MTR_0039s0080 28.416 461 318 5 37 490 46 501 6.14e-54 189
MS.gene042538.t1 MTR_5g010830 28.302 477 325 7 1 468 37 505 8.69e-54 189
MS.gene042538.t1 MTR_1g101500 28.508 449 312 3 37 480 72 516 4.86e-53 187
MS.gene042538.t1 MTR_5g067460 25.268 467 332 4 25 482 51 509 6.94e-50 179
MS.gene042538.t1 MTR_2g078990 34.364 291 188 2 173 461 4 293 6.56e-46 162
MS.gene042538.t1 MTR_4g027920 25.597 461 335 3 26 483 153 608 1.74e-45 168
MS.gene042538.t1 MTR_6g043810 24.111 506 358 8 5 490 7 506 1.56e-43 161
MS.gene042538.t1 MTR_6g043810 24.260 507 342 8 11 490 5 496 6.03e-43 159
MS.gene042538.t1 MTR_6g004220 29.338 317 212 4 1 313 8 316 2.71e-35 134
MS.gene042538.t1 MTR_2g079030 38.860 193 118 0 267 459 319 511 3.93e-35 138
MS.gene042538.t1 MTR_2g435930 23.722 489 340 5 15 480 5 483 3.85e-34 134
MS.gene042538.t1 MTR_6g088805 24.628 471 338 6 4 467 3 463 5.68e-33 131
MS.gene042538.t1 MTR_5g011540 25.911 494 335 8 15 490 28 508 9.52e-31 125
MS.gene042538.t1 MTR_8g102430 24.342 456 296 9 27 467 21 442 7.70e-30 121
MS.gene042538.t1 MTR_4g131290 38.983 59 36 0 101 159 13 71 8.63e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042538.t1 AT1G33110 62.917 480 175 1 4 480 7 486 0.0 640
MS.gene042538.t1 AT1G33100 61.426 477 184 0 4 480 7 483 0.0 629
MS.gene042538.t1 AT1G33090 61.042 480 184 1 4 480 7 486 0.0 612
MS.gene042538.t1 AT1G33080 59.504 484 193 1 2 482 5 488 0.0 608
MS.gene042538.t1 AT1G33080 60.645 465 180 1 2 463 5 469 0.0 593
MS.gene042538.t1 AT1G33110 64.484 397 138 1 4 397 7 403 0.0 527
MS.gene042538.t1 AT5G44050 47.890 474 246 1 6 478 16 489 3.90e-161 466
MS.gene042538.t1 AT5G65380 48.504 468 240 1 9 475 16 483 1.60e-160 464
MS.gene042538.t1 AT1G47530 46.407 487 253 2 1 482 1 484 7.15e-152 442
MS.gene042538.t1 AT1G12950 46.608 457 243 1 30 486 67 522 2.61e-146 429
MS.gene042538.t1 AT5G10420 47.569 473 246 2 9 480 18 489 6.29e-146 427
MS.gene042538.t1 AT3G26590 44.536 485 256 3 2 473 4 488 1.30e-145 426
MS.gene042538.t1 AT4G00350 46.171 444 238 1 30 473 88 530 1.88e-145 427
MS.gene042538.t1 AT5G17700 44.196 491 264 2 4 484 7 497 3.01e-145 426
MS.gene042538.t1 AT5G17700 44.196 491 264 2 4 484 7 497 3.01e-145 426
MS.gene042538.t1 AT1G23300 44.172 489 267 2 6 490 16 502 2.10e-144 424
MS.gene042538.t1 AT1G11670 45.175 487 253 4 3 475 10 496 3.13e-141 416
MS.gene042538.t1 AT1G61890 45.397 478 249 3 18 490 31 501 3.20e-141 415
MS.gene042538.t1 AT5G38030 45.133 452 247 1 30 481 45 495 3.24e-141 415
MS.gene042538.t1 AT3G03620 44.375 480 265 2 11 490 19 496 3.10e-140 413
MS.gene042538.t1 AT3G03620 44.375 480 265 2 11 490 19 496 3.10e-140 413
MS.gene042538.t1 AT1G61890 46.903 452 235 2 18 464 31 482 3.67e-137 405
MS.gene042538.t1 AT4G25640 44.353 487 260 3 4 482 2 485 5.12e-135 399
MS.gene042538.t1 AT4G25640 44.353 487 260 3 4 482 2 485 7.77e-135 399
MS.gene042538.t1 AT3G21690 46.930 456 241 1 21 475 43 498 4.15e-134 397
MS.gene042538.t1 AT1G47530 49.620 395 196 1 88 482 1 392 1.80e-133 392
MS.gene042538.t1 AT4G21910 43.226 465 254 4 18 475 39 500 1.20e-128 384
MS.gene042538.t1 AT4G21910 43.226 465 254 4 18 475 39 500 1.20e-128 384
MS.gene042538.t1 AT3G26590 47.409 386 203 0 88 473 1 386 4.10e-128 378
MS.gene042538.t1 AT4G21910 42.373 472 264 4 7 475 36 502 1.37e-127 381
MS.gene042538.t1 AT4G21910 42.373 472 264 4 7 475 36 502 1.37e-127 381
MS.gene042538.t1 AT1G61890 46.731 413 215 2 18 425 31 443 1.28e-121 364
MS.gene042538.t1 AT4G25640 44.851 437 230 3 4 432 2 435 4.10e-121 363
MS.gene042538.t1 AT4G21903 43.349 436 231 3 50 477 73 500 3.86e-119 359
MS.gene042538.t1 AT5G17700 44.557 395 218 1 4 397 7 401 3.31e-115 345
MS.gene042538.t1 AT3G59030 39.004 482 286 4 7 486 29 504 1.11e-114 348
MS.gene042538.t1 AT4G21903 41.722 453 244 4 50 490 73 517 1.45e-114 348
MS.gene042538.t1 AT4G21903 44.730 389 214 1 88 475 1 389 3.64e-113 340
MS.gene042538.t1 AT5G52450 35.642 491 292 6 2 485 9 482 1.05e-88 280
MS.gene042538.t1 AT2G34360 34.021 485 293 8 3 477 2 469 1.39e-84 269
MS.gene042538.t1 AT1G73700 35.991 464 285 3 26 484 20 476 4.04e-84 268
MS.gene042538.t1 AT3G59030 37.018 389 243 2 7 394 29 416 8.78e-84 265
MS.gene042538.t1 AT1G15150 36.364 462 277 8 30 482 31 484 4.52e-80 258
MS.gene042538.t1 AT1G15160 35.281 462 282 8 30 482 31 484 2.20e-77 251
MS.gene042538.t1 AT1G66780 35.831 427 269 4 47 470 52 476 2.64e-77 250
MS.gene042538.t1 AT1G66760 34.161 483 291 9 1 470 1 469 1.27e-76 248
MS.gene042538.t1 AT1G71140 32.972 461 298 5 16 470 12 467 2.35e-76 248
MS.gene042538.t1 AT1G66760 34.310 478 287 9 1 465 1 464 5.42e-75 244
MS.gene042538.t1 AT1G15170 35.938 448 274 6 30 470 34 475 6.54e-75 244
MS.gene042538.t1 AT1G64820 32.599 454 295 6 37 484 36 484 3.19e-73 240
MS.gene042538.t1 AT2G04080 32.704 477 300 7 1 470 6 468 3.41e-73 239
MS.gene042538.t1 AT2G04080 32.558 473 298 7 1 466 6 464 5.33e-71 233
MS.gene042538.t1 AT2G04040 32.883 444 293 3 30 470 27 468 1.25e-70 233
MS.gene042538.t1 AT1G15180 35.938 448 274 6 30 470 35 476 1.15e-68 228
MS.gene042538.t1 AT2G34360 31.042 480 272 8 3 477 2 427 4.96e-68 224
MS.gene042538.t1 AT3G23560 33.836 464 288 4 2 461 23 471 7.16e-68 225
MS.gene042538.t1 AT2G04070 33.708 445 288 5 30 470 27 468 8.72e-68 225
MS.gene042538.t1 AT3G23550 32.174 460 299 2 3 461 16 463 4.09e-67 223
MS.gene042538.t1 AT2G04050 31.692 467 300 5 17 470 8 468 1.09e-64 217
MS.gene042538.t1 AT1G15150 35.580 371 229 5 119 482 1 368 2.98e-64 213
MS.gene042538.t1 AT2G04100 32.812 448 288 6 30 470 30 471 5.48e-63 213
MS.gene042538.t1 AT1G15170 36.212 359 219 5 119 470 1 356 6.44e-62 206
MS.gene042538.t1 AT2G04100 32.735 446 287 6 30 468 30 469 2.09e-61 208
MS.gene042538.t1 AT2G04090 32.222 450 288 6 30 470 30 471 5.66e-61 207
MS.gene042538.t1 AT1G66760 34.795 342 210 5 131 465 1 336 2.51e-53 183
MS.gene042538.t1 AT1G66760 32.678 407 247 9 1 394 1 393 6.59e-50 176
MS.gene042538.t1 AT3G23560 33.753 397 244 4 2 394 23 404 7.89e-50 176
MS.gene042538.t1 AT2G38510 25.376 465 335 5 26 483 2 461 8.46e-50 177
MS.gene042538.t1 AT1G58340 25.843 445 321 3 29 468 57 497 8.91e-45 165
MS.gene042538.t1 AT5G19700 26.369 493 342 6 6 486 9 492 5.78e-41 154
MS.gene042538.t1 AT1G71870 22.587 487 354 6 25 490 19 503 1.21e-38 147
MS.gene042538.t1 AT5G52050 24.078 515 347 6 3 490 8 505 1.41e-37 144
MS.gene042538.t1 AT4G29140 25.641 468 328 7 11 467 36 494 1.80e-37 144
MS.gene042538.t1 AT4G23030 26.694 487 340 7 7 484 10 488 1.94e-36 141
MS.gene042538.t1 AT1G15180 34.629 283 175 5 119 394 1 280 2.06e-35 137
MS.gene042538.t1 AT5G49130 25.301 498 347 5 1 481 3 492 2.06e-34 135
MS.gene042538.t1 AT2G04066 39.844 128 77 0 343 470 36 163 5.78e-27 107
MS.gene042538.t1 AT4G22790 25.833 480 316 13 13 467 6 470 1.51e-25 109

Find 96 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATGATTTCAACTATTT+TGG 0.128485 7.4:-20460421 MS.gene042538:CDS
TTGACAATGAAATACGAATT+TGG 0.132006 7.4:-20460453 MS.gene042538:CDS
ATGAGTTATCACTAGGAAAA+AGG 0.222046 7.4:-20461959 MS.gene042538:CDS
GAACACCATCATTGCATTCT+TGG 0.238540 7.4:-20460517 MS.gene042538:CDS
ATTCCTGTTGGGGTTGTTCT+TGG 0.263732 7.4:-20458610 MS.gene042538:CDS
ACAATAAAAGCAAACATAAT+TGG 0.272274 7.4:+20460579 None:intergenic
GAGGAATGCATGAATAATTA+TGG 0.285003 7.4:+20460485 None:intergenic
GATGATATCACTTGGTTTCA+TGG 0.286945 7.4:-20459250 MS.gene042538:CDS
TGACAATGAAATACGAATTT+GGG 0.314225 7.4:-20460452 MS.gene042538:CDS
GCATATGGTGCAAAAGAATA+TGG 0.321833 7.4:-20460759 MS.gene042538:CDS
TGGCCTAAGAGAGTCAAAAT+TGG 0.339582 7.4:+20460651 None:intergenic
TTGCAGGAAACATCTCTCTT+TGG 0.351337 7.4:-20460608 MS.gene042538:CDS
ATCTGGATGGGAATGTTGTT+TGG 0.361940 7.4:-20458363 MS.gene042538:CDS
GCTATGGTACAACACAATAT+TGG 0.365741 7.4:-20459957 MS.gene042538:CDS
TTATTCATGCATTCCTCTCT+TGG 0.371620 7.4:-20460479 MS.gene042538:CDS
GGCCTAAGAGAGTCAAAATT+GGG 0.375165 7.4:+20460652 None:intergenic
ACATCATAGGTATTCCTGTT+GGG 0.378560 7.4:-20458621 MS.gene042538:CDS
ATAGGTTGTTATTACATCAT+AGG 0.385071 7.4:-20458634 MS.gene042538:CDS
TAATCACCTACAAAACCAAC+TGG 0.393658 7.4:-20458311 MS.gene042538:CDS
ATTTCAACAGGAGTGGCAAT+TGG 0.399246 7.4:-20458691 MS.gene042538:intron
AAATTGATGCACTATCTATA+TGG 0.406199 7.4:-20459898 MS.gene042538:intron
GAAAATCTTGTGAATATTGC+TGG 0.419464 7.4:+20461897 None:intergenic
ACTGCATTGGCGACCCTTTG+TGG 0.422921 7.4:-20460786 MS.gene042538:CDS
AAATATGAGTTGACCAATGT+TGG 0.433777 7.4:+20460386 None:intergenic
CTTCTGATATGCTTTCATCT+TGG 0.434393 7.4:+20460631 None:intergenic
GTCAAACTTTCCCTTTCAGC+TGG 0.437394 7.4:-20460291 MS.gene042538:CDS
TTTGTGATTGAAGGAATGTT+TGG 0.439478 7.4:+20460544 None:intergenic
ACAGGAGTGGCAATTGGAGC+AGG 0.444328 7.4:-20458685 MS.gene042538:intron
CAACATTGCACCAGCTGAAA+GGG 0.447324 7.4:+20460281 None:intergenic
GAAGAAGAACAAGTGCAGTT+AGG 0.451597 7.4:+20460694 None:intergenic
ACCCTTTGTGGACAAGCATA+TGG 0.455701 7.4:-20460774 MS.gene042538:CDS
TGTTCTTGCTTTGTGATTGA+AGG 0.457141 7.4:+20460535 None:intergenic
GAGAAACCTTGCCAAGTCTC+AGG 0.471152 7.4:+20460345 None:intergenic
TGCGGCCACCTCTTTATTCG+AGG 0.471701 7.4:+20458979 None:intergenic
TTACCAAGAACAACCCCAAC+AGG 0.474968 7.4:+20458607 None:intergenic
ATGAATATTGTAGTTGGGAA+TGG 0.482830 7.4:-20460811 MS.gene042538:intron
TAGCTCGCAAGCGTGTAAAC+AGG 0.483098 7.4:-20458166 MS.gene042538:CDS
AAATACGAATTTGGGATTGC+TGG 0.485502 7.4:-20460444 MS.gene042538:CDS
GGAATGAAACAAAGCTTATG+TGG 0.489956 7.4:-20461932 MS.gene042538:CDS
GAGTATATCTTCAAAGATCA+TGG 0.493939 7.4:-20460728 MS.gene042538:CDS
TGCAAAAGAATATGGAATGA+TGG 0.495159 7.4:-20460751 MS.gene042538:CDS
TTCTTGGTAATGTTATCCAT+TGG 0.504552 7.4:-20458594 MS.gene042538:CDS
TATTATGCAGTCTTGAGCTA+TGG 0.507079 7.4:-20459973 MS.gene042538:intron
AATATGAGTTGACCAATGTT+GGG 0.515656 7.4:+20460387 None:intergenic
ATTAACAGTCTCAACATCAA+TGG 0.515679 7.4:-20459282 MS.gene042538:intron
TGTGACAGTGCTTACGTCGT+TGG 0.516527 7.4:-20459065 MS.gene042538:CDS
CATCATAGGTATTCCTGTTG+GGG 0.518377 7.4:-20458620 MS.gene042538:CDS
TACATCATAGGTATTCCTGT+TGG 0.519474 7.4:-20458622 MS.gene042538:CDS
GTATCAAATGAGCTTGGTAA+AGG 0.521672 7.4:-20459118 MS.gene042538:CDS
CAAAACCAACTGGGATGAGC+AGG 0.522487 7.4:-20458301 MS.gene042538:intron
ACCATATGCTTGTCCACAAA+GGG 0.523264 7.4:+20460773 None:intergenic
TACTTTGTACCTGAGAGTAC+TGG 0.525061 7.4:+20458899 None:intergenic
TTTCGATAGGGTATCTGGAT+GGG 0.528960 7.4:-20458375 MS.gene042538:intron
ACAGTCTCAACATCAATGGA+TGG 0.534228 7.4:-20459278 MS.gene042538:intron
TTTCAGCTGGTGCAATGTTG+TGG 0.537720 7.4:-20460278 MS.gene042538:intron
CACCATATGCTTGTCCACAA+AGG 0.545263 7.4:+20460772 None:intergenic
GAAAGCATATCAGAAGTTGC+AGG 0.546308 7.4:-20460624 MS.gene042538:CDS
TCATATTTGTTACATGTGGT+TGG 0.546428 7.4:-20460371 MS.gene042538:CDS
TGGTGTCCTGAGACTTGGCA+AGG 0.562901 7.4:-20460351 MS.gene042538:CDS
GAAAGGGAAAGTTTGACGAC+AGG 0.566650 7.4:+20460297 None:intergenic
TCATACCTGCTCATCCCAGT+TGG 0.566874 7.4:+20458296 None:intergenic
GTGGTTGGTGTCCTGAGACT+TGG 0.570911 7.4:-20460356 MS.gene042538:CDS
AGCTGCCAAGAATGCAATGA+TGG 0.574760 7.4:+20460512 None:intergenic
CAGTCTCAACATCAATGGAT+GGG 0.575086 7.4:-20459277 MS.gene042538:CDS
ACAACATTGCACCAGCTGAA+AGG 0.577683 7.4:+20460280 None:intergenic
AGCCTACCTTGACTTGCCAA+TGG 0.579025 7.4:+20458578 None:intergenic
AGTGTTCAACCAGTACTCTC+AGG 0.579086 7.4:-20458908 MS.gene042538:intron
CACAACGATCGAGAACTTCG+CGG 0.579588 7.4:+20459084 None:intergenic
GCATATTGGATTCCCAACAT+TGG 0.580747 7.4:-20460399 MS.gene042538:CDS
AAAGAGGTGGCCGCAGCTGT+CGG 0.584660 7.4:-20458971 MS.gene042538:CDS
GTGCTTACGTCGTTGGCCAT+CGG 0.585684 7.4:-20459058 MS.gene042538:CDS
GTTGGGAATGGCAACTGCAT+TGG 0.585961 7.4:-20460799 MS.gene042538:CDS
CAACTCATATTTGTTACATG+TGG 0.589602 7.4:-20460375 MS.gene042538:CDS
GCAAAAGAATATGGAATGAT+GGG 0.591328 7.4:-20460750 MS.gene042538:CDS
GTTCGAGTATCAAATGAGCT+TGG 0.593317 7.4:-20459124 MS.gene042538:CDS
GTTCTCATTAGGTTCGCCAA+TGG 0.599406 7.4:-20461789 MS.gene042538:CDS
ACAGTAGCATATGTGAACAT+AGG 0.603815 7.4:-20458652 MS.gene042538:CDS
TGTTATCCATTGGCAAGTCA+AGG 0.605886 7.4:-20458584 MS.gene042538:intron
GAAGAAAATGAGTTATCACT+AGG 0.627440 7.4:-20461966 MS.gene042538:CDS
CAGGAGTGGCAATTGGAGCA+GGG 0.627575 7.4:-20458684 MS.gene042538:CDS
TTGATCAGTACTGTCCACGT+TGG 0.628549 7.4:+20458139 None:intergenic
AGTACTCACGAGAATACCAT+TGG 0.633680 7.4:+20461773 None:intergenic
GAATGAAACAAAGCTTATGT+GGG 0.634561 7.4:-20461931 MS.gene042538:CDS
CGATAATTCTCCGACAGCTG+CGG 0.636484 7.4:+20458961 None:intergenic
TTGGTTTCATGGCTGCAGCA+AGG 0.642144 7.4:-20459239 MS.gene042538:intron
TCATTGTCAAAAGCCAAGAG+AGG 0.649754 7.4:+20460466 None:intergenic
CTCGCAAGCGTGTAAACAGG+TGG 0.656106 7.4:-20458163 MS.gene042538:CDS
AGTATAGAGATCGATAACAA+AGG 0.663232 7.4:+20458938 None:intergenic
TTTCACCTCGAATAAAGAGG+TGG 0.663498 7.4:-20458984 MS.gene042538:CDS
GGGGTTGTGAAGACAAACAC+AGG 0.666387 7.4:+20460672 None:intergenic
TGGGAAATGATGATATCACT+TGG 0.667441 7.4:-20459258 MS.gene042538:CDS
GCCTAAGAGAGTCAAAATTG+GGG 0.673808 7.4:+20460653 None:intergenic
AATCACCTACAAAACCAACT+GGG 0.676987 7.4:-20458310 MS.gene042538:CDS
TGTAAACAGGTGGTCCAACG+TGG 0.700790 7.4:-20458153 MS.gene042538:CDS
TGAAACAAAGCTTATGTGGG+TGG 0.707571 7.4:-20461928 MS.gene042538:CDS
GAGTGGCAATTGGAGCAGGG+TGG 0.718007 7.4:-20458681 MS.gene042538:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGTTTGTTTAATTAATA+AGG + chr7.4:20461730-20461749 None:intergenic 10.0%
!! ATTTAATTAATATACATTGT+CGG + chr7.4:20461887-20461906 None:intergenic 10.0%
!! AAAAAATATGTTATCCATAA+AGG + chr7.4:20460084-20460103 None:intergenic 15.0%
!!! AAGTAAATGTAAATTTTCAA+TGG + chr7.4:20460969-20460988 None:intergenic 15.0%
!! AATAAAAATGGTTAGAACAT+GGG - chr7.4:20460676-20460695 MS.gene042538:CDS 20.0%
!! AATACCAAAACATCATATAA+GGG + chr7.4:20458779-20458798 None:intergenic 20.0%
!! ACAATAAAAGCAAACATAAT+TGG + chr7.4:20459547-20459566 None:intergenic 20.0%
!! ACTGTTAATAATAACATTTG+GGG + chr7.4:20460830-20460849 None:intergenic 20.0%
!! AGACTGTTAATAATAACATT+TGG + chr7.4:20460832-20460851 None:intergenic 20.0%
!! CATTTCAATGATTATGATTA+TGG - chr7.4:20458861-20458880 MS.gene042538:intron 20.0%
!! GACTGTTAATAATAACATTT+GGG + chr7.4:20460831-20460850 None:intergenic 20.0%
!! GTAAAATGAATATTGTAGTT+GGG - chr7.4:20459307-20459326 MS.gene042538:intron 20.0%
!! GTATAACTGTATATTAGTAA+AGG - chr7.4:20458885-20458904 MS.gene042538:intron 20.0%
!! TAATACCAAAACATCATATA+AGG + chr7.4:20458780-20458799 None:intergenic 20.0%
!! TGTAAAATGAATATTGTAGT+TGG - chr7.4:20459306-20459325 MS.gene042538:intron 20.0%
!! TTATTAAGATATTGTTAACG+AGG + chr7.4:20461864-20461883 None:intergenic 20.0%
!! TTTAATGCAATCATATCTAT+CGG - chr7.4:20458583-20458602 MS.gene042538:intron 20.0%
!!! AAACTTGTTAATTTTAAGGA+AGG - chr7.4:20458670-20458689 MS.gene042538:CDS 20.0%
!!! ATAATGAGAATTTTATTCCT+AGG - chr7.4:20460432-20460451 MS.gene042538:CDS 20.0%
!!! ATTAAACAAACATTTTCGAT+AGG - chr7.4:20461735-20461754 MS.gene042538:intron 20.0%
!!! CTCAAAACTTGTTAATTTTA+AGG - chr7.4:20458666-20458685 MS.gene042538:CDS 20.0%
!!! TTAAAATGTTGTCACTTTAT+TGG - chr7.4:20458940-20458959 MS.gene042538:CDS 20.0%
!!! TTAAACAAACATTTTCGATA+GGG - chr7.4:20461736-20461755 MS.gene042538:intron 20.0%
!!! TTTTTAAAAGAACTATGAAC+AGG - chr7.4:20459100-20459119 MS.gene042538:CDS 20.0%
! AAATTGATGCACTATCTATA+TGG - chr7.4:20460225-20460244 MS.gene042538:intron 25.0%
! AATTAAGGATGAGTTAATCT+TGG - chr7.4:20459929-20459948 MS.gene042538:CDS 25.0%
! AATTCTCATTATTGTATTCC+TGG + chr7.4:20460424-20460443 None:intergenic 25.0%
! ATACCAAAACATCATATAAG+GGG + chr7.4:20458778-20458797 None:intergenic 25.0%
! ATTCTCATTATTGTATTCCT+GGG + chr7.4:20460423-20460442 None:intergenic 25.0%
! CTGTTAATAATAACATTTGG+GGG + chr7.4:20460829-20460848 None:intergenic 25.0%
! GAATAAAAATGGTTAGAACA+TGG - chr7.4:20460675-20460694 MS.gene042538:CDS 25.0%
! GTAAGCATATAAGATGAAAA+AGG + chr7.4:20460943-20460962 None:intergenic 25.0%
! GTTTACTAATTTAAACTCCT+GGG - chr7.4:20460475-20460494 MS.gene042538:CDS 25.0%
! TAAGAAGAACAAAAACAGAA+AGG + chr7.4:20461093-20461112 None:intergenic 25.0%
! TACAATGAAATAGAAGTGAA+TGG - chr7.4:20460565-20460584 MS.gene042538:CDS 25.0%
! TCATTTCATTTCATTTCAAC+AGG - chr7.4:20461420-20461439 MS.gene042538:intron 25.0%
! TGACAATGAAATACGAATTT+GGG - chr7.4:20459671-20459690 MS.gene042538:intron 25.0%
! TGTTAATAATAACATTTGGG+GGG + chr7.4:20460828-20460847 None:intergenic 25.0%
! TGTTTACTAATTTAAACTCC+TGG - chr7.4:20460474-20460493 MS.gene042538:CDS 25.0%
! TTGACAATGAAATACGAATT+TGG - chr7.4:20459670-20459689 MS.gene042538:intron 25.0%
! TTTGATGCATGTTTGAATTA+AGG - chr7.4:20459914-20459933 MS.gene042538:CDS 25.0%
!! ATAGGTTGTTATTACATCAT+AGG - chr7.4:20461489-20461508 MS.gene042538:intron 25.0%
!! ATTATTAGTCAAGCTTTTGT+TGG - chr7.4:20458265-20458284 MS.gene042538:intron 25.0%
!! ATTCTAGAGATTAGTTGAAT+GGG - chr7.4:20458808-20458827 MS.gene042538:intron 25.0%
!! TGCAATGATTTCAACTATTT+TGG - chr7.4:20459702-20459721 MS.gene042538:intron 25.0%
!! TGTATCTTTTGTCAAAATCA+CGG - chr7.4:20459982-20460001 MS.gene042538:intron 25.0%
!! TGTGAATTAGTTTGATGAAT+TGG - chr7.4:20461268-20461287 MS.gene042538:intron 25.0%
!! TTCACAAGATTTTCAACTTT+TGG - chr7.4:20458235-20458254 MS.gene042538:intron 25.0%
!! TTTTAGCATTCAAAGATCTT+TGG - chr7.4:20459803-20459822 MS.gene042538:intron 25.0%
!!! ATATATTTTGACAAATACCC+AGG + chr7.4:20460495-20460514 None:intergenic 25.0%
!!! CACATTTTTGAAATATTCCT+AGG + chr7.4:20460452-20460471 None:intergenic 25.0%
!!! TTGATAAATTTTGAGAGACA+AGG + chr7.4:20458488-20458507 None:intergenic 25.0%
!!! TTTCAACTATTTTGGCATAT+TGG - chr7.4:20459710-20459729 MS.gene042538:intron 25.0%
AGTATAGAGATCGATAACAA+AGG + chr7.4:20461188-20461207 None:intergenic 30.0%
ATAAGATGAAAAAGGATGGA+TGG + chr7.4:20460935-20460954 None:intergenic 30.0%
ATGAATATTGTAGTTGGGAA+TGG - chr7.4:20459312-20459331 MS.gene042538:intron 30.0%
ATGAGTTATCACTAGGAAAA+AGG - chr7.4:20458164-20458183 MS.gene042538:CDS 30.0%
ATTAACAGTCTCAACATCAA+TGG - chr7.4:20460841-20460860 MS.gene042538:intron 30.0%
CATCAAACTAATTCACATAG+TGG + chr7.4:20461265-20461284 None:intergenic 30.0%
CCTTCAACAAAAATATACAG+AGG + chr7.4:20458980-20458999 None:intergenic 30.0%
GAAAATCTTGTGAATATTGC+TGG + chr7.4:20458229-20458248 None:intergenic 30.0%
GAAATGAGTATTCCTATGTT+TGG + chr7.4:20460759-20460778 None:intergenic 30.0%
GAAGAAAATGAGTTATCACT+AGG - chr7.4:20458157-20458176 MS.gene042538:CDS 30.0%
GAATGAAACAAAGCTTATGT+GGG - chr7.4:20458192-20458211 MS.gene042538:intron 30.0%
GAGATAAAAAATGCATTCCT+GGG + chr7.4:20460794-20460813 None:intergenic 30.0%
GAGGAATGCATGAATAATTA+TGG + chr7.4:20459641-20459660 None:intergenic 30.0%
GAGTATATCTTCAAAGATCA+TGG - chr7.4:20459395-20459414 MS.gene042538:intron 30.0%
GCAAAAGAATATGGAATGAT+GGG - chr7.4:20459373-20459392 MS.gene042538:intron 30.0%
GCATATAAGATGAAAAAGGA+TGG + chr7.4:20460939-20460958 None:intergenic 30.0%
TACTATTATGTCGTCCTTTA+TGG - chr7.4:20460067-20460086 MS.gene042538:intron 30.0%
TATTCCTATGTTTGGTATGA+TGG + chr7.4:20460751-20460770 None:intergenic 30.0%
TGAGTTATCACTAGGAAAAA+GGG - chr7.4:20458165-20458184 MS.gene042538:CDS 30.0%
TGCAAAAGAATATGGAATGA+TGG - chr7.4:20459372-20459391 MS.gene042538:intron 30.0%
TGGATGTAAAATTCATTCCT+TGG - chr7.4:20460388-20460407 MS.gene042538:CDS 30.0%
TTTGTGATTGAAGGAATGTT+TGG + chr7.4:20459582-20459601 None:intergenic 30.0%
! AAATATGAGTTGACCAATGT+TGG + chr7.4:20459740-20459759 None:intergenic 30.0%
! AATATGAGTTGACCAATGTT+GGG + chr7.4:20459739-20459758 None:intergenic 30.0%
! ACAGTTTTTATGCAAAACGT+GGG - chr7.4:20460609-20460628 MS.gene042538:CDS 30.0%
! ATCCGTCATTTAACAGTTTT+GGG - chr7.4:20459129-20459148 MS.gene042538:intron 30.0%
! ATTTTTATTCCCAAGAGAGA+GGG + chr7.4:20460665-20460684 None:intergenic 30.0%
! CAACTCATATTTGTTACATG+TGG - chr7.4:20459748-20459767 MS.gene042538:intron 30.0%
! GATGGGAAAATTTTATTCCA+AGG + chr7.4:20460643-20460662 None:intergenic 30.0%
! GATTCTAGAGATTAGTTGAA+TGG - chr7.4:20458807-20458826 MS.gene042538:intron 30.0%
! GCAATTTGAAATCAAGAAGA+GGG + chr7.4:20461579-20461598 None:intergenic 30.0%
! TATCCGTCATTTAACAGTTT+TGG - chr7.4:20459128-20459147 MS.gene042538:CDS 30.0%
! TATTTTCACCTCGAATAAAG+AGG - chr7.4:20461136-20461155 MS.gene042538:intron 30.0%
! TCATATTTGTTACATGTGGT+TGG - chr7.4:20459752-20459771 MS.gene042538:intron 30.0%
! TGCAATTTGAAATCAAGAAG+AGG + chr7.4:20461580-20461599 None:intergenic 30.0%
! TTCTAGAGATTAGTTGAATG+GGG - chr7.4:20458809-20458828 MS.gene042538:intron 30.0%
! TTGTTTTCACTGTTCTCATT+AGG - chr7.4:20458323-20458342 MS.gene042538:CDS 30.0%
!! CCTCTGTATATTTTTGTTGA+AGG - chr7.4:20458977-20458996 MS.gene042538:CDS 30.0%
!! GAATTTTTCAGTTTCGTGTA+TGG + chr7.4:20461622-20461641 None:intergenic 30.0%
!! TTCTTGGTAATGTTATCCAT+TGG - chr7.4:20461529-20461548 MS.gene042538:intron 30.0%
!!! ACAATATTGGTTCTTTTGAC+AGG - chr7.4:20460179-20460198 MS.gene042538:intron 30.0%
!!! ATGATGTTTTGGTATTAGAG+TGG - chr7.4:20458783-20458802 MS.gene042538:intron 30.0%
!!! CAATATTGGTTCTTTTGACA+GGG - chr7.4:20460180-20460199 MS.gene042538:intron 30.0%
!!! GGTGTTCTGTTTTTTCTTTT+TGG - chr7.4:20458691-20458710 MS.gene042538:intron 30.0%
AAGTGAATGGATACAAAACG+TGG - chr7.4:20460578-20460597 MS.gene042538:CDS 35.0%
AATCACCTACAAAACCAACT+GGG - chr7.4:20461813-20461832 MS.gene042538:CDS 35.0%
AATGAAAGGCTTCTTTCACT+TGG - chr7.4:20460334-20460353 MS.gene042538:CDS 35.0%
AATGAATCACATAGACTCCT+CGG + chr7.4:20458645-20458664 None:intergenic 35.0%
ACAGTAGCATATGTGAACAT+AGG - chr7.4:20461471-20461490 MS.gene042538:intron 35.0%
ACATCATAGGTATTCCTGTT+GGG - chr7.4:20461502-20461521 MS.gene042538:intron 35.0%
ACGATTAAATCATTCGTTGC+AGG - chr7.4:20461928-20461947 MS.gene042538:CDS 35.0%
AGTGAATGGATACAAAACGT+GGG - chr7.4:20460579-20460598 MS.gene042538:CDS 35.0%
ATGAAAAAGGATGGATGGAA+AGG + chr7.4:20460930-20460949 None:intergenic 35.0%
ATTCATAGCAATGTTGTGTC+TGG - chr7.4:20459235-20459254 MS.gene042538:intron 35.0%
ATTGCCTTGTGATGAATGAA+AGG - chr7.4:20460320-20460339 MS.gene042538:CDS 35.0%
CCTAGGAATAAATAATACCC+AGG + chr7.4:20460713-20460732 None:intergenic 35.0%
CCTGGGTATTATTTATTCCT+AGG - chr7.4:20460710-20460729 MS.gene042538:CDS 35.0%
CTCCCAAAACTGTTAAATGA+CGG + chr7.4:20459134-20459153 None:intergenic 35.0%
CTCTCTCTTGGGAATAAAAA+TGG - chr7.4:20460664-20460683 MS.gene042538:CDS 35.0%
CTTCTGATATGCTTTCATCT+TGG + chr7.4:20459495-20459514 None:intergenic 35.0%
GATGATATCACTTGGTTTCA+TGG - chr7.4:20460873-20460892 MS.gene042538:intron 35.0%
GCATATGGTGCAAAAGAATA+TGG - chr7.4:20459364-20459383 MS.gene042538:intron 35.0%
GCTATGGTACAACACAATAT+TGG - chr7.4:20460166-20460185 MS.gene042538:intron 35.0%
GGAATGAAACAAAGCTTATG+TGG - chr7.4:20458191-20458210 MS.gene042538:intron 35.0%
GGAGATAAAAAATGCATTCC+TGG + chr7.4:20460795-20460814 None:intergenic 35.0%
GTATCAAATGAGCTTGGTAA+AGG - chr7.4:20461005-20461024 MS.gene042538:intron 35.0%
TAAGGATGAGTTAATCTTGG+TGG - chr7.4:20459932-20459951 MS.gene042538:CDS 35.0%
TAATCACCTACAAAACCAAC+TGG - chr7.4:20461812-20461831 MS.gene042538:CDS 35.0%
TACATCATAGGTATTCCTGT+TGG - chr7.4:20461501-20461520 MS.gene042538:intron 35.0%
TATCACTAGGAAAAAGGGTT+TGG - chr7.4:20458170-20458189 MS.gene042538:CDS 35.0%
TATTATGCAGTCTTGAGCTA+TGG - chr7.4:20460150-20460169 MS.gene042538:intron 35.0%
TCATTTCATTTCAACAGGAG+TGG - chr7.4:20461425-20461444 MS.gene042538:intron 35.0%
TGAAAAAGGATGGATGGAAA+GGG + chr7.4:20460929-20460948 None:intergenic 35.0%
TGGGAAATGATGATATCACT+TGG - chr7.4:20460865-20460884 MS.gene042538:intron 35.0%
TGGTTAGAACATGGGTAAAA+TGG - chr7.4:20460684-20460703 MS.gene042538:CDS 35.0%
TGTTCCATCATACCAAACAT+AGG - chr7.4:20460744-20460763 MS.gene042538:CDS 35.0%
TGTTCTTGCTTTGTGATTGA+AGG + chr7.4:20459591-20459610 None:intergenic 35.0%
TTATTCATGCATTCCTCTCT+TGG - chr7.4:20459644-20459663 MS.gene042538:intron 35.0%
! AAATACGAATTTGGGATTGC+TGG - chr7.4:20459679-20459698 MS.gene042538:intron 35.0%
! CAAGCTTTTGTTGGTCATAT+TGG - chr7.4:20458274-20458293 MS.gene042538:intron 35.0%
! CAAGGAGAAACATAGTTTTC+AGG + chr7.4:20458470-20458489 None:intergenic 35.0%
! CAGATTCATTCAAGTTAGCA+CGG + chr7.4:20460126-20460145 None:intergenic 35.0%
! CAGTGCATTTGTTACATTGA+TGG + chr7.4:20460035-20460054 None:intergenic 35.0%
! CATTTTTATTCCCAAGAGAG+AGG + chr7.4:20460666-20460685 None:intergenic 35.0%
! GACAGTTTTTATGCAAAACG+TGG - chr7.4:20460608-20460627 MS.gene042538:CDS 35.0%
! GGAAGCATACATATATGCAA+AGG + chr7.4:20461709-20461728 None:intergenic 35.0%
! TCACCCCTTATATGATGTTT+TGG - chr7.4:20458772-20458791 MS.gene042538:intron 35.0%
! TCTAGAGATTAGTTGAATGG+GGG - chr7.4:20458810-20458829 MS.gene042538:intron 35.0%
!! AACATTTTCGATAGGGTATC+TGG - chr7.4:20461743-20461762 MS.gene042538:intron 35.0%
AAACGTGGGTTCATATTCCT+TGG - chr7.4:20460623-20460642 MS.gene042538:CDS 40.0%
ACACATATTGAAGACGTGTG+TGG - chr7.4:20459193-20459212 MS.gene042538:intron 40.0%
ACAGTCTCAACATCAATGGA+TGG - chr7.4:20460845-20460864 MS.gene042538:intron 40.0%
ACCATATGCTTGTCCACAAA+GGG + chr7.4:20459353-20459372 None:intergenic 40.0%
AGGGAACATGAAAAATGCTG+AGG - chr7.4:20460199-20460218 MS.gene042538:intron 40.0%
AGTACTCACGAGAATACCAT+TGG + chr7.4:20458353-20458372 None:intergenic 40.0%
ATCTGGATGGGAATGTTGTT+TGG - chr7.4:20461760-20461779 MS.gene042538:intron 40.0%
ATTTCAACAGGAGTGGCAAT+TGG - chr7.4:20461432-20461451 MS.gene042538:intron 40.0%
ATTTCCCATTCTCTATTCCC+AGG - chr7.4:20460774-20460793 MS.gene042538:CDS 40.0%
CAAAAACAGAAAGGATCCGA+TGG + chr7.4:20461084-20461103 None:intergenic 40.0%
CAGTCTCAACATCAATGGAT+GGG - chr7.4:20460846-20460865 MS.gene042538:intron 40.0%
CATCATAGGTATTCCTGTTG+GGG - chr7.4:20461503-20461522 MS.gene042538:intron 40.0%
CATTCCTGGGAATAGAGAAT+GGG + chr7.4:20460781-20460800 None:intergenic 40.0%
GAAAAAGGATGGATGGAAAG+GGG + chr7.4:20460928-20460947 None:intergenic 40.0%
GAACACCATCATTGCATTCT+TGG - chr7.4:20459606-20459625 MS.gene042538:intron 40.0%
GAAGAAGAACAAGTGCAGTT+AGG + chr7.4:20459432-20459451 None:intergenic 40.0%
GAAGCCTTTCATTCATCACA+AGG + chr7.4:20460327-20460346 None:intergenic 40.0%
GCATATTGGATTCCCAACAT+TGG - chr7.4:20459724-20459743 MS.gene042538:intron 40.0%
GCCTAAGAGAGTCAAAATTG+GGG + chr7.4:20459473-20459492 None:intergenic 40.0%
GGAACAAAATCAACATGCCT+AGG + chr7.4:20460730-20460749 None:intergenic 40.0%
GGCCTAAGAGAGTCAAAATT+GGG + chr7.4:20459474-20459493 None:intergenic 40.0%
GTGAATGGATACAAAACGTG+GGG - chr7.4:20460580-20460599 MS.gene042538:CDS 40.0%
GTTCGAGTATCAAATGAGCT+TGG - chr7.4:20460999-20461018 MS.gene042538:intron 40.0%
TCATTGTCAAAAGCCAAGAG+AGG + chr7.4:20459660-20459679 None:intergenic 40.0%
TGAAACAAAGCTTATGTGGG+TGG - chr7.4:20458195-20458214 MS.gene042538:intron 40.0%
TGGCCTAAGAGAGTCAAAAT+TGG + chr7.4:20459475-20459494 None:intergenic 40.0%
TGTTATCCATTGGCAAGTCA+AGG - chr7.4:20461539-20461558 MS.gene042538:intron 40.0%
TTCCTGGGAAAGTTATTCCA+AGG + chr7.4:20460408-20460427 None:intergenic 40.0%
TTCCTTGGAATAACTTTCCC+AGG - chr7.4:20460403-20460422 MS.gene042538:CDS 40.0%
TTGCAGGAAACATCTCTCTT+TGG - chr7.4:20459515-20459534 MS.gene042538:intron 40.0%
TTTCACCTCGAATAAAGAGG+TGG - chr7.4:20461139-20461158 MS.gene042538:intron 40.0%
! ACATGGGTAAAATGGATTCC+TGG - chr7.4:20460692-20460711 MS.gene042538:CDS 40.0%
! CATGGGTAAAATGGATTCCT+GGG - chr7.4:20460693-20460712 MS.gene042538:CDS 40.0%
! CTAGAGATTAGTTGAATGGG+GGG - chr7.4:20458811-20458830 MS.gene042538:intron 40.0%
! GAAAGCATATCAGAAGTTGC+AGG - chr7.4:20459499-20459518 MS.gene042538:intron 40.0%
! TACTTTGTACCTGAGAGTAC+TGG + chr7.4:20461227-20461246 None:intergenic 40.0%
! TTTCGATAGGGTATCTGGAT+GGG - chr7.4:20461748-20461767 MS.gene042538:intron 40.0%
!! ACTTGGCTTGCTAAAGATCA+AGG - chr7.4:20460351-20460370 MS.gene042538:CDS 40.0%
!! AGTGCTGCATAGAGTTTTAG+AGG + chr7.4:20459172-20459191 None:intergenic 40.0%
!! CTTGGCTTGCTAAAGATCAA+GGG - chr7.4:20460352-20460371 MS.gene042538:CDS 40.0%
!! TCAAGGGAGTGTTTGTTTCA+TGG - chr7.4:20460368-20460387 MS.gene042538:CDS 40.0%
!! TTTTCGATAGGGTATCTGGA+TGG - chr7.4:20461747-20461766 MS.gene042538:intron 40.0%
!!! ACCCCAATTTTGACTCTCTT+AGG - chr7.4:20459469-20459488 MS.gene042538:intron 40.0%
ACAACATTGCACCAGCTGAA+AGG + chr7.4:20459846-20459865 None:intergenic 45.0%
ACCCTTTGTGGACAAGCATA+TGG - chr7.4:20459349-20459368 MS.gene042538:intron 45.0%
AGCTGCCAAGAATGCAATGA+TGG + chr7.4:20459614-20459633 None:intergenic 45.0%
AGTGTTCAACCAGTACTCTC+AGG - chr7.4:20461215-20461234 MS.gene042538:intron 45.0%
ATCCATTGGCAAGTCAAGGT+AGG - chr7.4:20461543-20461562 MS.gene042538:intron 45.0%
ATTCCTGTTGGGGTTGTTCT+TGG - chr7.4:20461513-20461532 MS.gene042538:intron 45.0%
ATTGAGACACACCAAATCCG+AGG - chr7.4:20458625-20458644 MS.gene042538:CDS 45.0%
CAACATTGCACCAGCTGAAA+GGG + chr7.4:20459845-20459864 None:intergenic 45.0%
CAAGACAAGCAAGTTCAACG+TGG + chr7.4:20458437-20458456 None:intergenic 45.0%
CACCATATGCTTGTCCACAA+AGG + chr7.4:20459354-20459373 None:intergenic 45.0%
GAAAGGGAAAGTTTGACGAC+AGG + chr7.4:20459829-20459848 None:intergenic 45.0%
GCATTCCTGGGAATAGAGAA+TGG + chr7.4:20460782-20460801 None:intergenic 45.0%
GTAGGTATCTGTGACGTGTT+TGG + chr7.4:20458526-20458545 None:intergenic 45.0%
GTCAAACTTTCCCTTTCAGC+TGG - chr7.4:20459832-20459851 MS.gene042538:intron 45.0%
GTTCTCATTAGGTTCGCCAA+TGG - chr7.4:20458334-20458353 MS.gene042538:CDS 45.0%
TATTCCCAAGAGAGAGGGAT+GGG + chr7.4:20460660-20460679 None:intergenic 45.0%
TCACATAGACTCCTCGGATT+TGG + chr7.4:20458639-20458658 None:intergenic 45.0%
TTACCAAGAACAACCCCAAC+AGG + chr7.4:20461519-20461538 None:intergenic 45.0%
TTATTCCCAAGAGAGAGGGA+TGG + chr7.4:20460661-20460680 None:intergenic 45.0%
TTGATCAGTACTGTCCACGT+TGG + chr7.4:20461987-20462006 None:intergenic 45.0%
TTTCAGCTGGTGCAATGTTG+TGG - chr7.4:20459845-20459864 MS.gene042538:intron 45.0%
! ATTTTCCCATCCCTCTCTCT+TGG - chr7.4:20460652-20460671 MS.gene042538:CDS 45.0%
! TTTTCCCATCCCTCTCTCTT+GGG - chr7.4:20460653-20460672 MS.gene042538:CDS 45.0%
!! GGGGGTTTTGATGACTTTGT+AGG - chr7.4:20458829-20458848 MS.gene042538:intron 45.0%
!! TATCATCAGTGTCGACGTGT+AGG + chr7.4:20458544-20458563 None:intergenic 45.0%
!! TGCAAAGGCACTTCAGTTGA+AGG + chr7.4:20461694-20461713 None:intergenic 45.0%
!!! CTCATCCCAGTTGGTTTTGT+AGG + chr7.4:20461821-20461840 None:intergenic 45.0%
!! TTATTATATTATTAAAAAGA+AGG + chr7.4:20458411-20458430 None:intergenic 5.0%
AGGATGGATGGAAAGGGGAT+CGG + chr7.4:20460923-20460942 None:intergenic 50.0%
CAAAACCAACTGGGATGAGC+AGG - chr7.4:20461822-20461841 MS.gene042538:CDS 50.0%
CACAACGATCGAGAACTTCG+CGG + chr7.4:20461042-20461061 None:intergenic 50.0%
CGATAATTCTCCGACAGCTG+CGG + chr7.4:20461165-20461184 None:intergenic 50.0%
GAGAAACCTTGCCAAGTCTC+AGG + chr7.4:20459781-20459800 None:intergenic 50.0%
GGGGTTGTGAAGACAAACAC+AGG + chr7.4:20459454-20459473 None:intergenic 50.0%
GTTGGGAATGGCAACTGCAT+TGG - chr7.4:20459324-20459343 MS.gene042538:intron 50.0%
TAGCTCGCAAGCGTGTAAAC+AGG - chr7.4:20461957-20461976 MS.gene042538:CDS 50.0%
TCATACCTGCTCATCCCAGT+TGG + chr7.4:20461830-20461849 None:intergenic 50.0%
TGTAAACAGGTGGTCCAACG+TGG - chr7.4:20461970-20461989 MS.gene042538:CDS 50.0%
TGTGACAGTGCTTACGTCGT+TGG - chr7.4:20461058-20461077 MS.gene042538:intron 50.0%
TTGGTTTCATGGCTGCAGCA+AGG - chr7.4:20460884-20460903 MS.gene042538:intron 50.0%
!! AGCCTACCTTGACTTGCCAA+TGG + chr7.4:20461548-20461567 None:intergenic 50.0%
!! CATTTGGGGGGCGTTTGTTT+CGG + chr7.4:20460816-20460835 None:intergenic 50.0%
ACAGGAGTGGCAATTGGAGC+AGG - chr7.4:20461438-20461457 MS.gene042538:intron 55.0%
CAGGAGTGGCAATTGGAGCA+GGG - chr7.4:20461439-20461458 MS.gene042538:intron 55.0%
CTCGCAAGCGTGTAAACAGG+TGG - chr7.4:20461960-20461979 MS.gene042538:CDS 55.0%
GTGCTTACGTCGTTGGCCAT+CGG - chr7.4:20461065-20461084 MS.gene042538:intron 55.0%
TGCGGCCACCTCTTTATTCG+AGG + chr7.4:20461147-20461166 None:intergenic 55.0%
TGGTGTCCTGAGACTTGGCA+AGG - chr7.4:20459772-20459791 MS.gene042538:intron 55.0%
! ACTGCATTGGCGACCCTTTG+TGG - chr7.4:20459337-20459356 MS.gene042538:intron 55.0%
! GTGGTTGGTGTCCTGAGACT+TGG - chr7.4:20459767-20459786 MS.gene042538:intron 55.0%
AAAGAGGTGGCCGCAGCTGT+CGG - chr7.4:20461152-20461171 MS.gene042538:intron 60.0%
GAGTGGCAATTGGAGCAGGG+TGG - chr7.4:20461442-20461461 MS.gene042538:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 20458109 20462036 20458109 ID=MS.gene042538
chr7.4 mRNA 20458109 20462036 20458109 ID=MS.gene042538.t1;Parent=MS.gene042538
chr7.4 exon 20461782 20462036 20461782 ID=MS.gene042538.t1.exon1;Parent=MS.gene042538.t1
chr7.4 CDS 20461782 20462036 20461782 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
chr7.4 exon 20460279 20460820 20460279 ID=MS.gene042538.t1.exon2;Parent=MS.gene042538.t1
chr7.4 CDS 20460279 20460820 20460279 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
chr7.4 exon 20459899 20459985 20459899 ID=MS.gene042538.t1.exon3;Parent=MS.gene042538.t1
chr7.4 CDS 20459899 20459985 20459899 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
chr7.4 exon 20459240 20459296 20459240 ID=MS.gene042538.t1.exon4;Parent=MS.gene042538.t1
chr7.4 CDS 20459240 20459296 20459240 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
chr7.4 exon 20458909 20459147 20458909 ID=MS.gene042538.t1.exon5;Parent=MS.gene042538.t1
chr7.4 CDS 20458909 20459147 20458909 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
chr7.4 exon 20458585 20458703 20458585 ID=MS.gene042538.t1.exon6;Parent=MS.gene042538.t1
chr7.4 CDS 20458585 20458703 20458585 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
chr7.4 exon 20458302 20458388 20458302 ID=MS.gene042538.t1.exon7;Parent=MS.gene042538.t1
chr7.4 CDS 20458302 20458388 20458302 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
chr7.4 exon 20458109 20458195 20458109 ID=MS.gene042538.t1.exon8;Parent=MS.gene042538.t1
chr7.4 CDS 20458109 20458195 20458109 ID=cds.MS.gene042538.t1;Parent=MS.gene042538.t1
Gene Sequence

>MS.gene042538

ATGGAAAGAGATCTAAAACAGAATCTACTACAGAAAAAATCAGAACAAGAAGAAAATGAGTTATCACTAGGAAAAAGGGTTTGGAATGAAACAAAGCTTATGTGGGTGGTTGCAGCACCAGCAATATTCACAAGATTTTCAACTTTTGGTATTCAAATTATTAGTCAAGCTTTTGTTGGTCATATTGGTTCAAGAGAACTCGCTGCTTTTGCTCTTGTTTTCACTGTTCTCATTAGGTTCGCCAATGGTATTCTCGTGAGTACTTCATTTTCTCATCATTTTTCAACTTTTTTCTTTGACCTTCTTTTTAATAATATAATAATAACCACGTTGAACTTGCTTGTCTTGTCTTGTGTTACCTGAAAACTATGTTTCTCCTTGTCTCTCAAAATTTATCAAACACAAGTATTGACTCCAAACACGTCACAGATACCTACACGTCGACACTGATGATAATTTGAGAGAATTACATACTTTAATGCAATCATATCTATCGGAAGCTGACACTTGTCTGATATTGAGACACACCAAATCCGAGGAGTCTATGTGATTCATTGCTCAAAACTTGTTAATTTTAAGGAAGGTGTTCTGTTTTTTCTTTTTGGTATAGATACTAGTAGTTAGTACTTCTCTGTTTCTTCAGACAAAAGTTCAAAAAAAGTTTCACCCCTTATATGATGTTTTGGTATTAGAGTGGTGATTCTAGAGATTAGTTGAATGGGGGGTTTTGATGACTTTGTAGGAGAAAGTTTCATTTCAATGATTATGATTATGGTGTATAACTGTATATTAGTAAAGGTTGCAGCTTTTGCACTTTTTCTATTAAAACATTTAAAATGTTGTCACTTTATTGGTGTGTGAACACTTTCCTCTGTATATTTTTGTTGAAGGACACTTGATAAAGATTTAAAAATTGAAACAATCGATAACTTTCATCTAGAAAAAGTTGTTTTAGCATGTTTCAATGAGTTGAATACAGAAGAAGAAGTTGTTTTTAAAAGAACTATGAACAGGAAAAATATCCGTCATTTAACAGTTTTGGGAGAAATGATTACAATTTCCTCTAAAACTCTATGCAGCACTTACACATATTGAAGACGTGTGTGGTTTTCGACACTGACACGACATTCATAGCAATGTTGTGTCTGGTGTCTATGCTTCATAGAACATACTAAGTACTAACACAAGTTAATTGTTTGTAAAATGAATATTGTAGTTGGGAATGGCAACTGCATTGGCGACCCTTTGTGGACAAGCATATGGTGCAAAAGAATATGGAATGATGGGAGTATATCTTCAAAGATCATGGATAGTTTTATTCCTAACTGCACTTGTTCTTCTTCCTGTGTTTGTCTTCACAACCCCAATTTTGACTCTCTTAGGCCAAGATGAAAGCATATCAGAAGTTGCAGGAAACATCTCTCTTTGGTCAATTCCAATTATGTTTGCTTTTATTGTCTCATTCACTTGCCAAACATTCCTTCAATCACAAAGCAAGAACACCATCATTGCATTCTTGGCAGCTTTTTCCATAATTATTCATGCATTCCTCTCTTGGCTTTTGACAATGAAATACGAATTTGGGATTGCTGGTGCAATGATTTCAACTATTTTGGCATATTGGATTCCCAACATTGGTCAACTCATATTTGTTACATGTGGTTGGTGTCCTGAGACTTGGCAAGGTTTCTCATTTTTAGCATTCAAAGATCTTTGGCCTGTCGTCAAACTTTCCCTTTCAGCTGGTGCAATGTTGTGGTGAGTTCTTTGCAAATGCAATTACTGATGACTTGATTTGAATTTCATTTGATGCATGTTTGAATTAAGGATGAGTTAATCTTGGTGGCATCGTAGTTTTGTTGAAGCTCGTAAGTGTATCTTTTGTCAAAATCACGGCGATACAATGTGATTAAGTCAAACTAACCATCAATGTAACAAATGCACTGAATCAATGAGCTTACTATTATGTCGTCCTTTATGGATAACATATTTTTTGTGTGAACAAATATATATGCCGTGCTAACTTGAATGAATCTGTTTATATTATGCAGTCTTGAGCTATGGTACAACACAATATTGGTTCTTTTGACAGGGAACATGAAAAATGCTGAGGTTGAAATTGATGCACTATCTATATGGTAATGCTTCTGCTCAGTAACACTTTTATTAAAATTTATGATTCTGTTTCTACTTTATGTTATTTGTTATTGTATTGCCTTGTGATGAATGAAAGGCTTCTTTCACTTGGCTTGCTAAAGATCAAGGGAGTGTTTGTTTCATGGATGTAAAATTCATTCCTTGGAATAACTTTCCCAGGAATACAATAATGAGAATTTTATTCCTAGGAATATTTCAAAAATGTGTTTGTTTACTAATTTAAACTCCTGGGTATTTGTCAAAATATATACTTGTTTTATAAATAAAAAAAGAAAAGAAAAATCATCAATTAACTAATTTACAATGAAATAGAAGTGAATGGATACAAAACGTGGGGTATAAGACAGTTTTTATGCAAAACGTGGGTTCATATTCCTTGGAATAAAATTTTCCCATCCCTCTCTCTTGGGAATAAAAATGGTTAGAACATGGGTAAAATGGATTCCTGGGTATTATTTATTCCTAGGCATGTTGATTTTGTTCCATCATACCAAACATAGGAATACTCATTTCCCATTCTCTATTCCCAGGAATGCATTTTTTATCTCCGAAACAAACGCCCCCCAAATGTTATTATTAACAGTCTCAACATCAATGGATGGGAAATGATGATATCACTTGGTTTCATGGCTGCAGCAAGGTAATAACATTGTTCCGATCCCCTTTCCATCCATCCTTTTTCATCTTATATGCTTACTTACCATTGAAAATTTACATTTACTTATTGTGTAGTGTTCGAGTATCAAATGAGCTTGGTAAAGGAAGCGCGAAAGCCGCGAAGTTCTCGATCGTTGTGACAGTGCTTACGTCGTTGGCCATCGGATCCTTTCTGTTTTTGTTCTTCTTATTTTTTAGAGAAAGACTTGCTTATATTTTCACCTCGAATAAAGAGGTGGCCGCAGCTGTCGGAGAATTATCGCCTTTGTTATCGATCTCTATACTATTAAACAGTGTTCAACCAGTACTCTCAGGTACAAAGTAGTATTTACAACATTTCCACTATGTGAATTAGTTTGATGAATTGGTTCAAGACTTGTGCAAATTAATCTTCAATGCTATATAACAACAGTTATACATTGAATTTGTCAGTTTTTCTCGTGTATATATTTATACTTGTCAAAGAATATGAAAGAAATGAAAGCAAAAATGTAATTTCATTTCATTTCATTTCAACAGGAGTGGCAATTGGAGCAGGGTGGCAAAGCACAGTAGCATATGTGAACATAGGTTGTTATTACATCATAGGTATTCCTGTTGGGGTTGTTCTTGGTAATGTTATCCATTGGCAAGTCAAGGTAGGCTTTTATCATCCCTCTTCTTGATTTCAAATTGCACTTATATTAAGCACTGATGCCATACACGAAACTGAAAAATTCAATTATTGAATGTTATACATGAGTTAATGTCGTGTCAGATACTCACACACCTTCAACTGAAGTGCCTTTGCATATATGTATGCTTCCTTATTAATTAAACAAACATTTTCGATAGGGTATCTGGATGGGAATGTTGTTTGGAACATTGATTCAAACTATAGTTCTACTTATAATCACCTACAAAACCAACTGGGATGAGCAGGTATGATGACTTATTTTCCTCGTTAACAATATCTTAATAACCGACAATGTATATTAATTAAATTCTTATAGATATACATCTAATACGATTAAATCATTCGTTGCAGGTGACTGTAGCTCGCAAGCGTGTAAACAGGTGGTCCAACGTGGACAGTACTGATCAAGAAACAAAAACTAACTTAATTGAAAAATAG
Protein sequence

>MS.gene042538.t1

MERDLKQNLLQKKSEQEENELSLGKRVWNETKLMWVVAAPAIFTRFSTFGIQIISQAFVGHIGSRELAAFALVFTVLIRFANGILLGMATALATLCGQAYGAKEYGMMGVYLQRSWIVLFLTALVLLPVFVFTTPILTLLGQDESISEVAGNISLWSIPIMFAFIVSFTCQTFLQSQSKNTIIAFLAAFSIIIHAFLSWLLTMKYEFGIAGAMISTILAYWIPNIGQLIFVTCGWCPETWQGFSFLAFKDLWPVVKLSLSAGAMLCLELWYNTILVLLTGNMKNAEVEIDALSICLNINGWEMMISLGFMAAASVRVSNELGKGSAKAAKFSIVVTVLTSLAIGSFLFLFFLFFRERLAYIFTSNKEVAAAVGELSPLLSISILLNSVQPVLSGVAIGAGWQSTVAYVNIGCYYIIGIPVGVVLGNVIHWQVKGIWMGMLFGTLIQTIVLLIITYKTNWDEQVTVARKRVNRWSNVDSTDQETKTNLIEK