Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05669.t1 | KEH34139.1 | 56.5 | 124 | 34 | 3 | 1 | 110 | 1 | 118 | 5.20E-23 | 117.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05669.t1 | A0A072UWC0 | 56.5 | 124 | 34 | 3 | 1 | 110 | 1 | 118 | 3.8e-23 | 117.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene05669 | 0.81053 | 1.10E-50 | -1.69E-46 |
| MS.gene05329 | MS.gene05669 | 0.802805 | 4.71E-49 | -1.69E-46 |
| MS.gene05669 | MS.gene05676 | 0.843187 | 1.63E-58 | -1.69E-46 |
| MS.gene05669 | MS.gene05678 | 0.81677 | 4.66E-52 | -1.69E-46 |
| MS.gene05669 | MS.gene068494 | 0.813783 | 2.15E-51 | -1.69E-46 |
| MS.gene05669 | MS.gene22612 | 0.812315 | 4.51E-51 | -1.69E-46 |
| MS.gene05669 | MS.gene22613 | 0.805712 | 1.17E-49 | -1.69E-46 |
| MS.gene05669 | MS.gene47552 | 0.805439 | 1.33E-49 | -1.69E-46 |
| MS.gene05669 | MS.gene49642 | 0.815853 | 7.47E-52 | -1.69E-46 |
| MS.gene05669 | MS.gene75887 | 0.840144 | 1.04E-57 | -1.69E-46 |
| MS.gene05669 | MS.gene80877 | 0.827306 | 1.68E-54 | -1.69E-46 |
| MS.gene05669 | MS.gene97534 | 0.803673 | 3.12E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05669.t1 | MTR_3g462080 | 62.903 | 124 | 26 | 3 | 1 | 110 | 1 | 118 | 3.39e-44 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAGCGACTCCCTCATTTC+TGG | 0.281072 | 3.2:-51508741 | None:intergenic |
| AACATGACCGGATGCGGAAT+TGG | 0.340972 | 3.2:-51508895 | None:intergenic |
| GGAGTTCGTGATCTCGTGTT+AGG | 0.342593 | 3.2:+51508657 | MS.gene05669:CDS |
| GAGTTCGTGATCTCGTGTTA+GGG | 0.419998 | 3.2:+51508658 | MS.gene05669:CDS |
| CATTTCTGGTGTGATTACTA+AGG | 0.440969 | 3.2:-51508727 | None:intergenic |
| CAAGCCTCCAATTCCGCATC+CGG | 0.449798 | 3.2:+51508888 | MS.gene05669:CDS |
| GGGAGTCGCTTAACCGTCAT+TGG | 0.467747 | 3.2:+51508751 | MS.gene05669:CDS |
| TAGAGGTGGGGATGGAGTCA+AGG | 0.474938 | 3.2:+51508692 | MS.gene05669:CDS |
| CGGATGGAGATGGAGTTGTC+AGG | 0.475179 | 3.2:+51508610 | MS.gene05669:CDS |
| TTAGGGGACGTCGAGAATAG+AGG | 0.481029 | 3.2:+51508675 | MS.gene05669:CDS |
| TTCAAGAAGGGAATGGAAGG+TGG | 0.482368 | 3.2:+51508774 | MS.gene05669:CDS |
| TGAAGTATGTCGGATGGAGA+TGG | 0.483593 | 3.2:+51508600 | None:intergenic |
| TCAAGAAGGGAATGGAAGGT+GGG | 0.498967 | 3.2:+51508775 | MS.gene05669:CDS |
| CGGATGCGGAATTGGAGGCT+TGG | 0.507109 | 3.2:-51508887 | None:intergenic |
| GGAGTTGGTGATATCGAGGA+TGG | 0.511833 | 3.2:+51508636 | MS.gene05669:CDS |
| GGAGATGGAGTTGTCAGGGA+TGG | 0.527525 | 3.2:+51508615 | MS.gene05669:CDS |
| GTCGAGAATAGAGGTGGGGA+TGG | 0.531827 | 3.2:+51508684 | MS.gene05669:CDS |
| TTCCCTTCTTGAACCAATGA+CGG | 0.536074 | 3.2:-51508764 | None:intergenic |
| TAACCGTCATTGGTTCAAGA+AGG | 0.539237 | 3.2:+51508761 | MS.gene05669:CDS |
| AGCACCAACATGACCGGATG+CGG | 0.541690 | 3.2:-51508901 | None:intergenic |
| TAGTAATCACACCAGAAATG+AGG | 0.542592 | 3.2:+51508730 | MS.gene05669:CDS |
| ATGACCGGATGCGGAATTGG+AGG | 0.548162 | 3.2:-51508892 | None:intergenic |
| AATAGAAGCACCAACATGAC+CGG | 0.566507 | 3.2:-51508907 | None:intergenic |
| AGTAATCACACCAGAAATGA+GGG | 0.575012 | 3.2:+51508731 | MS.gene05669:CDS |
| AACCGTCATTGGTTCAAGAA+GGG | 0.580822 | 3.2:+51508762 | MS.gene05669:CDS |
| TGGTTCAAGAAGGGAATGGA+AGG | 0.589388 | 3.2:+51508771 | MS.gene05669:CDS |
| TTAAAGATGGCGGATGAAGG+TGG | 0.596893 | 3.2:-51508801 | None:intergenic |
| GGAGTTGTCAGGGATGGAGT+TGG | 0.610996 | 3.2:+51508621 | MS.gene05669:CDS |
| AATTCCGCATCCGGTCATGT+TGG | 0.615116 | 3.2:+51508897 | MS.gene05669:CDS |
| TCATTGGTTCAAGAAGGGAA+TGG | 0.632874 | 3.2:+51508767 | MS.gene05669:CDS |
| GGGGACGTCGAGAATAGAGG+TGG | 0.644629 | 3.2:+51508678 | MS.gene05669:CDS |
| GGATGGAGATGGAGTTGTCA+GGG | 0.649124 | 3.2:+51508611 | MS.gene05669:CDS |
| GGATGGAGTTGGTGATATCG+AGG | 0.651118 | 3.2:+51508632 | MS.gene05669:CDS |
| AGGCTTGGAGAGCAGAACCT+CGG | 0.654121 | 3.2:-51508872 | None:intergenic |
| GGGACGTCGAGAATAGAGGT+GGG | 0.655686 | 3.2:+51508679 | MS.gene05669:CDS |
| AGTTCGTGATCTCGTGTTAG+GGG | 0.674252 | 3.2:+51508659 | MS.gene05669:CDS |
| GGACGTCGAGAATAGAGGTG+GGG | 0.676100 | 3.2:+51508680 | MS.gene05669:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTCCATGTTTTTTAAAGA+TGG | - | chr3.2:51508817-51508836 | None:intergenic | 25.0% |
| !!! | TCCATGTTTTTTAAAGATGG+CGG | - | chr3.2:51508814-51508833 | None:intergenic | 30.0% |
| AGTAATCACACCAGAAATGA+GGG | + | chr3.2:51508731-51508750 | MS.gene05669:CDS | 35.0% | |
| TAGTAATCACACCAGAAATG+AGG | + | chr3.2:51508730-51508749 | MS.gene05669:CDS | 35.0% | |
| ! | TCCGCCATCTTTAAAAAACA+TGG | + | chr3.2:51508810-51508829 | MS.gene05669:CDS | 35.0% |
| !! | CATTTCTGGTGTGATTACTA+AGG | - | chr3.2:51508730-51508749 | None:intergenic | 35.0% |
| !!! | TTTTTAAAGATGGCGGATGA+AGG | - | chr3.2:51508807-51508826 | None:intergenic | 35.0% |
| TTCCCTTCTTGAACCAATGA+CGG | - | chr3.2:51508767-51508786 | None:intergenic | 40.0% | |
| !! | AACCGTCATTGGTTCAAGAA+GGG | + | chr3.2:51508762-51508781 | MS.gene05669:CDS | 40.0% |
| !! | AATAGAAGCACCAACATGAC+CGG | - | chr3.2:51508910-51508929 | None:intergenic | 40.0% |
| !! | TAACCGTCATTGGTTCAAGA+AGG | + | chr3.2:51508761-51508780 | MS.gene05669:CDS | 40.0% |
| !! | TCATTGGTTCAAGAAGGGAA+TGG | + | chr3.2:51508767-51508786 | MS.gene05669:CDS | 40.0% |
| !!! | GCTCTAGATTGTTTTAACCG+AGG | + | chr3.2:51508855-51508874 | MS.gene05669:CDS | 40.0% |
| AGTTCGTGATCTCGTGTTAG+GGG | + | chr3.2:51508659-51508678 | MS.gene05669:CDS | 45.0% | |
| GAGTTCGTGATCTCGTGTTA+GGG | + | chr3.2:51508658-51508677 | MS.gene05669:CDS | 45.0% | |
| TCAAGAAGGGAATGGAAGGT+GGG | + | chr3.2:51508775-51508794 | MS.gene05669:CDS | 45.0% | |
| TGGTTCAAGAAGGGAATGGA+AGG | + | chr3.2:51508771-51508790 | MS.gene05669:CDS | 45.0% | |
| TTAAGCGACTCCCTCATTTC+TGG | - | chr3.2:51508744-51508763 | None:intergenic | 45.0% | |
| TTCAAGAAGGGAATGGAAGG+TGG | + | chr3.2:51508774-51508793 | MS.gene05669:CDS | 45.0% | |
| !! | TTAAAGATGGCGGATGAAGG+TGG | - | chr3.2:51508804-51508823 | None:intergenic | 45.0% |
| AACATGACCGGATGCGGAAT+TGG | - | chr3.2:51508898-51508917 | None:intergenic | 50.0% | |
| AATTCCGCATCCGGTCATGT+TGG | + | chr3.2:51508897-51508916 | MS.gene05669:CDS | 50.0% | |
| GGAGTTCGTGATCTCGTGTT+AGG | + | chr3.2:51508657-51508676 | MS.gene05669:CDS | 50.0% | |
| GGATGGAGATGGAGTTGTCA+GGG | + | chr3.2:51508611-51508630 | MS.gene05669:CDS | 50.0% | |
| TTAGGGGACGTCGAGAATAG+AGG | + | chr3.2:51508675-51508694 | MS.gene05669:CDS | 50.0% | |
| !! | GGAGTTGGTGATATCGAGGA+TGG | + | chr3.2:51508636-51508655 | MS.gene05669:CDS | 50.0% |
| !! | GGATGGAGTTGGTGATATCG+AGG | + | chr3.2:51508632-51508651 | MS.gene05669:CDS | 50.0% |
| AGCACCAACATGACCGGATG+CGG | - | chr3.2:51508904-51508923 | None:intergenic | 55.0% | |
| AGGCTTGGAGAGCAGAACCT+CGG | - | chr3.2:51508875-51508894 | None:intergenic | 55.0% | |
| ATGACCGGATGCGGAATTGG+AGG | - | chr3.2:51508895-51508914 | None:intergenic | 55.0% | |
| CAAGCCTCCAATTCCGCATC+CGG | + | chr3.2:51508888-51508907 | MS.gene05669:CDS | 55.0% | |
| CGGATGGAGATGGAGTTGTC+AGG | + | chr3.2:51508610-51508629 | MS.gene05669:CDS | 55.0% | |
| GGACGTCGAGAATAGAGGTG+GGG | + | chr3.2:51508680-51508699 | MS.gene05669:CDS | 55.0% | |
| GGAGATGGAGTTGTCAGGGA+TGG | + | chr3.2:51508615-51508634 | MS.gene05669:CDS | 55.0% | |
| GGAGTTGTCAGGGATGGAGT+TGG | + | chr3.2:51508621-51508640 | MS.gene05669:CDS | 55.0% | |
| GGGACGTCGAGAATAGAGGT+GGG | + | chr3.2:51508679-51508698 | MS.gene05669:CDS | 55.0% | |
| GGGAGTCGCTTAACCGTCAT+TGG | + | chr3.2:51508751-51508770 | MS.gene05669:CDS | 55.0% | |
| GTCGAGAATAGAGGTGGGGA+TGG | + | chr3.2:51508684-51508703 | MS.gene05669:CDS | 55.0% | |
| TAGAGGTGGGGATGGAGTCA+AGG | + | chr3.2:51508692-51508711 | MS.gene05669:CDS | 55.0% | |
| CGGATGCGGAATTGGAGGCT+TGG | - | chr3.2:51508890-51508909 | None:intergenic | 60.0% | |
| GGGGACGTCGAGAATAGAGG+TGG | + | chr3.2:51508678-51508697 | MS.gene05669:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 51508606 | 51508938 | 51508606 | ID=MS.gene05669 |
| chr3.2 | mRNA | 51508606 | 51508938 | 51508606 | ID=MS.gene05669.t1;Parent=MS.gene05669 |
| chr3.2 | exon | 51508606 | 51508938 | 51508606 | ID=MS.gene05669.t1.exon1;Parent=MS.gene05669.t1 |
| chr3.2 | CDS | 51508606 | 51508938 | 51508606 | ID=cds.MS.gene05669.t1;Parent=MS.gene05669.t1 |
| Gene Sequence |
| Protein sequence |
