AlfalfaGEDB | MS.gene75887

M. sativa cultivar XinJiangDaYe / MS.gene75887

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75887.t1	GAU36621.1	66	53	17	1	1	53	1	52	6.40E-09	69.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75887.t1	A0A2Z6MYR6	66.0	53	17	1	1	53	1	52	4.6e-09	69.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene75887	0.811185	7.95E-51	-1.69E-46
MS.gene050026	MS.gene75887	0.824843	6.48E-54	-1.69E-46
MS.gene050750	MS.gene75887	0.809849	1.54E-50	-1.69E-46
MS.gene050953	MS.gene75887	0.806696	7.26E-50	-1.69E-46
MS.gene051198	MS.gene75887	0.817099	3.93E-52	-1.69E-46
MS.gene052239	MS.gene75887	-0.813181	2.92E-51	-1.69E-46
MS.gene052447	MS.gene75887	0.855776	5.03E-62	-1.69E-46
MS.gene053166	MS.gene75887	0.810488	1.12E-50	-1.69E-46
MS.gene05329	MS.gene75887	0.836411	9.51E-57	-1.69E-46
MS.gene05415	MS.gene75887	0.847852	8.90E-60	-1.69E-46
MS.gene05423	MS.gene75887	0.803277	3.77E-49	-1.69E-46
MS.gene054651	MS.gene75887	0.805843	1.10E-49	-1.69E-46
MS.gene055228	MS.gene75887	0.83446	2.96E-56	-1.69E-46
MS.gene055818	MS.gene75887	0.804365	2.24E-49	-1.69E-46
MS.gene056174	MS.gene75887	0.822888	1.86E-53	-1.69E-46
MS.gene056272	MS.gene75887	0.811936	5.46E-51	-1.69E-46
MS.gene05669	MS.gene75887	0.840144	1.04E-57	-1.69E-46
MS.gene057444	MS.gene75887	0.809357	1.97E-50	-1.69E-46
MS.gene058010	MS.gene75887	0.802204	6.27E-49	-1.69E-46
MS.gene058294	MS.gene75887	0.808839	2.54E-50	-1.69E-46
MS.gene058979	MS.gene75887	0.81274	3.64E-51	-1.69E-46
MS.gene059459	MS.gene75887	0.801655	8.13E-49	-1.69E-46
MS.gene059460	MS.gene75887	0.809116	2.22E-50	-1.69E-46
MS.gene059584	MS.gene75887	0.814987	1.16E-51	-1.69E-46
MS.gene060011	MS.gene75887	0.816169	6.35E-52	-1.69E-46
MS.gene060167	MS.gene75887	0.80669	7.28E-50	-1.69E-46
MS.gene060521	MS.gene75887	0.800438	1.44E-48	-1.69E-46
MS.gene060634	MS.gene75887	-0.814072	1.86E-51	-1.69E-46
MS.gene060918	MS.gene75887	0.812027	5.21E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75887.t1	MTR_2g011420	94.340	53	2	1	1	53	1	52	9.06e-29	101
MS.gene75887.t1	MTR_2g011420	94.340	53	2	1	1	53	1	52	8.36e-28	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 15 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCCACAAATGGAAATTTCT+TGG	0.278625	2.4:+42683970	None:intergenic
AAATTACCCCACGCAGATTC+TGG	0.280776	2.4:+42684046	None:intergenic
GCAGAATAAAGAACCGGTTT+TGG	0.296006	2.4:-42684098	MS.gene75887:CDS
GCAGCAGCAATCTTCTATTC+AGG	0.365842	2.4:-42684002	MS.gene75887:CDS
ATTTCCATTTGTGGGTGATA+AGG	0.396431	2.4:-42683963	MS.gene75887:CDS
GCCAAGAAATTTCCATTTGT+GGG	0.422209	2.4:-42683971	MS.gene75887:CDS
AGCCAAGAAATTTCCATTTG+TGG	0.451303	2.4:-42683972	MS.gene75887:CDS
CATTTGTGGGTGATAAGGTA+AGG	0.478241	2.4:-42683958	MS.gene75887:CDS
ACTTCCCCATCATCCAAAAC+CGG	0.521167	2.4:+42684085	None:intergenic
CTTACCTTATCACCCACAAA+TGG	0.525327	2.4:+42683959	None:intergenic
TGATGGGGAAGTAGAGTATG+AGG	0.548779	2.4:-42684074	MS.gene75887:CDS
GGCTGGTCCAGAATCTGCGT+GGG	0.566158	2.4:-42684053	MS.gene75887:CDS
GGGGAAGTAGAGTATGAGGC+TGG	0.604667	2.4:-42684070	MS.gene75887:CDS
AGGCTGGTCCAGAATCTGCG+TGG	0.639324	2.4:-42684054	MS.gene75887:CDS
GCTGGTCCAGAATCTGCGTG+GGG	0.726249	2.4:-42684052	MS.gene75887:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACCCACAAATGGAAATTTCT+TGG	+	chr2.4:42684085-42684104	None:intergenic	35.0%
	AGCCAAGAAATTTCCATTTG+TGG	-	chr2.4:42684080-42684099	MS.gene75887:CDS	35.0%
	ATTTCCATTTGTGGGTGATA+AGG	-	chr2.4:42684089-42684108	MS.gene75887:CDS	35.0%
	GCCAAGAAATTTCCATTTGT+GGG	-	chr2.4:42684081-42684100	MS.gene75887:CDS	35.0%
	CATTTGTGGGTGATAAGGTA+AGG	-	chr2.4:42684094-42684113	MS.gene75887:CDS	40.0%
	CTTACCTTATCACCCACAAA+TGG	+	chr2.4:42684096-42684115	None:intergenic	40.0%
!!	GCAGAATAAAGAACCGGTTT+TGG	-	chr2.4:42683954-42683973	MS.gene75887:CDS	40.0%
!!!	AAAGAACCGGTTTTGGATGA+TGG	-	chr2.4:42683961-42683980	MS.gene75887:CDS	40.0%
!!!	AAGAACCGGTTTTGGATGAT+GGG	-	chr2.4:42683962-42683981	MS.gene75887:CDS	40.0%
	AAATTACCCCACGCAGATTC+TGG	+	chr2.4:42684009-42684028	None:intergenic	45.0%
	ACTTCCCCATCATCCAAAAC+CGG	+	chr2.4:42683970-42683989	None:intergenic	45.0%
	GCAGCAGCAATCTTCTATTC+AGG	-	chr2.4:42684050-42684069	MS.gene75887:CDS	45.0%
	TGATGGGGAAGTAGAGTATG+AGG	-	chr2.4:42683978-42683997	MS.gene75887:CDS	45.0%
!	ATCTGCGTGGGGTAATTTTG+AGG	-	chr2.4:42684011-42684030	MS.gene75887:CDS	45.0%
!!!	AGAACCGGTTTTGGATGATG+GGG	-	chr2.4:42683963-42683982	MS.gene75887:CDS	45.0%
	GGGGAAGTAGAGTATGAGGC+TGG	-	chr2.4:42683982-42684001	MS.gene75887:CDS	55.0%
	GCTGGTCCAGAATCTGCGTG+GGG	-	chr2.4:42684000-42684019	MS.gene75887:CDS	60.0%
!	AGGCTGGTCCAGAATCTGCG+TGG	-	chr2.4:42683998-42684017	MS.gene75887:CDS	60.0%
!	GGCTGGTCCAGAATCTGCGT+GGG	-	chr2.4:42683999-42684018	MS.gene75887:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	42683952	42684122	42683952	ID=MS.gene75887
chr2.4	mRNA	42683952	42684122	42683952	ID=MS.gene75887.t1;Parent=MS.gene75887
chr2.4	exon	42683952	42684122	42683952	ID=MS.gene75887.t1.exon1;Parent=MS.gene75887.t1
chr2.4	CDS	42683952	42684122	42683952	ID=cds.MS.gene75887.t1;Parent=MS.gene75887.t1

Gene Sequence	>MS.gene75887 ATGCAGAATAAAGAACCGGTTTTGGATGATGGGGAAGTAGAGTATGAGGCTGGTCCAGAATCTGCGTGGGGTAATTTTGAGGATGATGATGATGTAATGCAGCAGCAATCTTCTATTCAGGATGATGAAGCCAAGAAATTTCCATTTGTGGGTGATAAGGTAAGGCCCTAA
Protein sequence	>MS.gene75887.t1 MQNKEPVLDDGEVEYEAGPESAWGNFEDDDDVMQQQSSIQDDEAKKFPFVGDKVRP