Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80877.t1 | GAU36621.1 | 66 | 53 | 17 | 1 | 1 | 53 | 1 | 52 | 6.00E-09 | 69.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80877.t1 | A0A2Z6MYR6 | 66.0 | 53 | 17 | 1 | 1 | 53 | 1 | 52 | 4.3e-09 | 69.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene80877 | 0.817728 | 2.84E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene80877 | 0.803665 | 3.13E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene80877 | 0.829989 | 3.78E-55 | -1.69E-46 |
| MS.gene050750 | MS.gene80877 | 0.822572 | 2.20E-53 | -1.69E-46 |
| MS.gene050953 | MS.gene80877 | 0.823336 | 1.46E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene80877 | 0.83203 | 1.19E-55 | -1.69E-46 |
| MS.gene052239 | MS.gene80877 | -0.822982 | 1.77E-53 | -1.69E-46 |
| MS.gene052447 | MS.gene80877 | 0.845789 | 3.26E-59 | -1.69E-46 |
| MS.gene053006 | MS.gene80877 | 0.809742 | 1.63E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene80877 | 0.819614 | 1.06E-52 | -1.69E-46 |
| MS.gene053201 | MS.gene80877 | 0.803193 | 3.92E-49 | -1.69E-46 |
| MS.gene05329 | MS.gene80877 | 0.825426 | 4.72E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene80877 | 0.802532 | 5.36E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene80877 | 0.852405 | 4.72E-61 | -1.69E-46 |
| MS.gene054405 | MS.gene80877 | 0.815728 | 7.97E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene80877 | 0.816445 | 5.51E-52 | -1.69E-46 |
| MS.gene055228 | MS.gene80877 | 0.845617 | 3.63E-59 | -1.69E-46 |
| MS.gene055818 | MS.gene80877 | 0.8139 | 2.03E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene80877 | 0.835418 | 1.70E-56 | -1.69E-46 |
| MS.gene056272 | MS.gene80877 | 0.815289 | 9.98E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene80877 | 0.804003 | 2.66E-49 | -1.69E-46 |
| MS.gene05669 | MS.gene80877 | 0.827306 | 1.68E-54 | -1.69E-46 |
| MS.gene056873 | MS.gene80877 | 0.806751 | 7.06E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene80877 | 0.802004 | 6.89E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene80877 | 0.820731 | 5.87E-53 | -1.69E-46 |
| MS.gene057598 | MS.gene80877 | 0.809161 | 2.17E-50 | -1.69E-46 |
| MS.gene05761 | MS.gene80877 | 0.80915 | 2.18E-50 | -1.69E-46 |
| MS.gene058009 | MS.gene80877 | 0.804959 | 1.68E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene80877 | 0.822129 | 2.79E-53 | -1.69E-46 |
| MS.gene058294 | MS.gene80877 | 0.815993 | 6.95E-52 | -1.69E-46 |
| MS.gene058979 | MS.gene80877 | 0.83423 | 3.38E-56 | -1.69E-46 |
| MS.gene05925 | MS.gene80877 | 0.801037 | 1.09E-48 | -1.69E-46 |
| MS.gene059459 | MS.gene80877 | 0.808104 | 3.65E-50 | -1.69E-46 |
| MS.gene059460 | MS.gene80877 | 0.808188 | 3.50E-50 | -1.69E-46 |
| MS.gene059584 | MS.gene80877 | 0.829261 | 5.68E-55 | -1.69E-46 |
| MS.gene060011 | MS.gene80877 | 0.841194 | 5.51E-58 | -1.69E-46 |
| MS.gene060167 | MS.gene80877 | 0.82538 | 4.84E-54 | -1.69E-46 |
| MS.gene060310 | MS.gene80877 | 0.800504 | 1.40E-48 | -1.69E-46 |
| MS.gene060521 | MS.gene80877 | 0.804281 | 2.33E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene80877 | -0.814106 | 1.82E-51 | -1.69E-46 |
| MS.gene060918 | MS.gene80877 | 0.824411 | 8.19E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80877.t1 | MTR_2g011420 | 94.340 | 53 | 2 | 1 | 1 | 53 | 1 | 52 | 9.36e-29 | 101 |
| MS.gene80877.t1 | MTR_2g011420 | 94.340 | 53 | 2 | 1 | 1 | 53 | 1 | 52 | 8.00e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCCACAAATGGAAATTTCT+TGG | 0.278625 | 2.4:+42713186 | None:intergenic |
| AAATTACCCCACGCAGATTC+TGG | 0.280776 | 2.4:+42713262 | None:intergenic |
| GCAGAATAAAGAACCGGTTT+TGG | 0.296006 | 2.4:-42713314 | MS.gene80877:CDS |
| GCAGCAGCAATCTTCTATTC+AGG | 0.365842 | 2.4:-42713218 | MS.gene80877:CDS |
| ATTTCCATTTGTGGGTGATA+AGG | 0.396431 | 2.4:-42713179 | None:intergenic |
| GCCAAGAAATTTCCATTTGT+GGG | 0.422209 | 2.4:-42713187 | MS.gene80877:CDS |
| AGCCAAGAAATTTCCATTTG+TGG | 0.451303 | 2.4:-42713188 | MS.gene80877:CDS |
| ACTTCCCCATCATCCAAAAC+CGG | 0.521167 | 2.4:+42713301 | None:intergenic |
| CTTACCTTATCACCCACAAA+TGG | 0.525327 | 2.4:+42713175 | None:intergenic |
| TGATGGGGAAGTAGAGTATG+AGG | 0.548779 | 2.4:-42713290 | MS.gene80877:CDS |
| GGCTGGTCCAGAATCTGCGT+GGG | 0.566158 | 2.4:-42713269 | MS.gene80877:CDS |
| GGGGAAGTAGAGTATGAGGC+TGG | 0.604667 | 2.4:-42713286 | MS.gene80877:CDS |
| AGGCTGGTCCAGAATCTGCG+TGG | 0.639324 | 2.4:-42713270 | MS.gene80877:CDS |
| GCTGGTCCAGAATCTGCGTG+GGG | 0.726249 | 2.4:-42713268 | MS.gene80877:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCCACAAATGGAAATTTCT+TGG | + | chr2.4:42713313-42713332 | None:intergenic | 35.0% | |
| AGCCAAGAAATTTCCATTTG+TGG | - | chr2.4:42713308-42713327 | MS.gene80877:CDS | 35.0% | |
| GCCAAGAAATTTCCATTTGT+GGG | - | chr2.4:42713309-42713328 | MS.gene80877:CDS | 35.0% | |
| !! | GCAGAATAAAGAACCGGTTT+TGG | - | chr2.4:42713182-42713201 | MS.gene80877:CDS | 40.0% |
| !!! | AAAGAACCGGTTTTGGATGA+TGG | - | chr2.4:42713189-42713208 | MS.gene80877:CDS | 40.0% |
| !!! | AAGAACCGGTTTTGGATGAT+GGG | - | chr2.4:42713190-42713209 | MS.gene80877:CDS | 40.0% |
| AAATTACCCCACGCAGATTC+TGG | + | chr2.4:42713237-42713256 | None:intergenic | 45.0% | |
| ACTTCCCCATCATCCAAAAC+CGG | + | chr2.4:42713198-42713217 | None:intergenic | 45.0% | |
| GCAGCAGCAATCTTCTATTC+AGG | - | chr2.4:42713278-42713297 | MS.gene80877:CDS | 45.0% | |
| TGATGGGGAAGTAGAGTATG+AGG | - | chr2.4:42713206-42713225 | MS.gene80877:CDS | 45.0% | |
| ! | ATCTGCGTGGGGTAATTTTG+AGG | - | chr2.4:42713239-42713258 | MS.gene80877:CDS | 45.0% |
| !!! | AGAACCGGTTTTGGATGATG+GGG | - | chr2.4:42713191-42713210 | MS.gene80877:CDS | 45.0% |
| GGGGAAGTAGAGTATGAGGC+TGG | - | chr2.4:42713210-42713229 | MS.gene80877:CDS | 55.0% | |
| GCTGGTCCAGAATCTGCGTG+GGG | - | chr2.4:42713228-42713247 | MS.gene80877:CDS | 60.0% | |
| ! | AGGCTGGTCCAGAATCTGCG+TGG | - | chr2.4:42713226-42713245 | MS.gene80877:CDS | 60.0% |
| ! | GGCTGGTCCAGAATCTGCGT+GGG | - | chr2.4:42713227-42713246 | MS.gene80877:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 42713180 | 42713338 | 42713180 | ID=MS.gene80877 |
| chr2.4 | mRNA | 42713180 | 42713338 | 42713180 | ID=MS.gene80877.t1;Parent=MS.gene80877 |
| chr2.4 | exon | 42713180 | 42713338 | 42713180 | ID=MS.gene80877.t1.exon1;Parent=MS.gene80877.t1 |
| chr2.4 | CDS | 42713180 | 42713338 | 42713180 | ID=cds.MS.gene80877.t1;Parent=MS.gene80877.t1 |
| Gene Sequence |
| Protein sequence |
