AlfalfaGEDB | MS.gene08889

M. sativa cultivar XinJiangDaYe / MS.gene08889

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08889.t1	XP_013446516.1	97.2	71	2	0	1	71	96	166	1.10E-34	155.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08889.t1	Q9LU91	54.2	59	23	2	1	55	57	115	1.1e-09	63.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08889.t1	A0A072TUL9	97.2	71	2	0	1	71	96	166	8.0e-35	155.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene08889	0.819107	1.38E-52	-1.69E-46
MS.gene04917	MS.gene08889	0.820132	8.06E-53	-1.69E-46
MS.gene049208	MS.gene08889	0.803622	3.19E-49	-1.69E-46
MS.gene049674	MS.gene08889	0.828927	6.85E-55	-1.69E-46
MS.gene049685	MS.gene08889	0.844912	5.64E-59	-1.69E-46
MS.gene049703	MS.gene08889	-0.809909	1.50E-50	-1.69E-46
MS.gene050084	MS.gene08889	0.806659	7.39E-50	-1.69E-46
MS.gene050085	MS.gene08889	0.83253	8.97E-56	-1.69E-46
MS.gene050114	MS.gene08889	0.808246	3.40E-50	-1.69E-46
MS.gene050317	MS.gene08889	0.833017	6.79E-56	-1.69E-46
MS.gene050359	MS.gene08889	0.82822	1.01E-54	-1.69E-46
MS.gene050361	MS.gene08889	0.830452	2.91E-55	-1.69E-46
MS.gene050384	MS.gene08889	0.822844	1.91E-53	-1.69E-46
MS.gene050386	MS.gene08889	0.835609	1.52E-56	-1.69E-46
MS.gene050646	MS.gene08889	0.835231	1.89E-56	-1.69E-46
MS.gene050654	MS.gene08889	0.851232	1.01E-60	-1.69E-46
MS.gene050689	MS.gene08889	0.837719	4.40E-57	-1.69E-46
MS.gene050752	MS.gene08889	0.823437	1.39E-53	-1.69E-46
MS.gene050838	MS.gene08889	0.846024	2.82E-59	-1.69E-46
MS.gene050941	MS.gene08889	0.821011	5.06E-53	-1.69E-46
MS.gene051003	MS.gene08889	0.834817	2.41E-56	-1.69E-46
MS.gene051234	MS.gene08889	0.810353	1.20E-50	-1.69E-46
MS.gene051245	MS.gene08889	0.816608	5.07E-52	-1.69E-46
MS.gene051343	MS.gene08889	0.8312	1.91E-55	-1.69E-46
MS.gene051381	MS.gene08889	0.817412	3.34E-52	-1.69E-46
MS.gene051856	MS.gene08889	0.835326	1.79E-56	-1.69E-46
MS.gene051902	MS.gene08889	0.817922	2.56E-52	-1.69E-46
MS.gene052242	MS.gene08889	-0.829912	3.95E-55	-1.69E-46
MS.gene052307	MS.gene08889	0.822126	2.80E-53	-1.69E-46
MS.gene052335	MS.gene08889	0.815089	1.10E-51	-1.69E-46
MS.gene052342	MS.gene08889	0.820783	5.72E-53	-1.69E-46
MS.gene052434	MS.gene08889	0.835221	1.90E-56	-1.69E-46
MS.gene052527	MS.gene08889	0.804178	2.45E-49	-1.69E-46
MS.gene052609	MS.gene08889	0.801923	7.16E-49	-1.69E-46
MS.gene052932	MS.gene08889	0.81822	2.20E-52	-1.69E-46
MS.gene053058	MS.gene08889	0.863442	2.48E-64	-1.69E-46
MS.gene053109	MS.gene08889	0.806004	1.02E-49	-1.69E-46
MS.gene053167	MS.gene08889	0.801846	7.42E-49	-1.69E-46
MS.gene053359	MS.gene08889	0.805078	1.59E-49	-1.69E-46
MS.gene053381	MS.gene08889	0.806611	7.56E-50	-1.69E-46
MS.gene05344	MS.gene08889	-0.801095	1.06E-48	-1.69E-46
MS.gene053456	MS.gene08889	0.813612	2.34E-51	-1.69E-46
MS.gene053564	MS.gene08889	0.805551	1.26E-49	-1.69E-46
MS.gene053590	MS.gene08889	0.803563	3.28E-49	-1.69E-46
MS.gene053879	MS.gene08889	0.814082	1.85E-51	-1.69E-46
MS.gene054403	MS.gene08889	0.818833	1.59E-52	-1.69E-46
MS.gene054823	MS.gene08889	0.812964	3.25E-51	-1.69E-46
MS.gene055311	MS.gene08889	0.854088	1.55E-61	-1.69E-46
MS.gene055313	MS.gene08889	0.819645	1.04E-52	-1.69E-46
MS.gene055314	MS.gene08889	0.831985	1.22E-55	-1.69E-46
MS.gene055513	MS.gene08889	0.809029	2.32E-50	-1.69E-46
MS.gene056045	MS.gene08889	0.833925	4.03E-56	-1.69E-46
MS.gene056097	MS.gene08889	0.853619	2.12E-61	-1.69E-46
MS.gene056181	MS.gene08889	0.854607	1.10E-61	-1.69E-46
MS.gene056752	MS.gene08889	0.821025	5.03E-53	-1.69E-46
MS.gene056764	MS.gene08889	0.802111	6.55E-49	-1.69E-46
MS.gene056787	MS.gene08889	0.851614	7.91E-61	-1.69E-46
MS.gene056849	MS.gene08889	0.823205	1.57E-53	-1.69E-46
MS.gene057057	MS.gene08889	0.828914	6.90E-55	-1.69E-46
MS.gene057234	MS.gene08889	0.814637	1.39E-51	-1.69E-46
MS.gene057452	MS.gene08889	-0.834922	2.26E-56	-1.69E-46
MS.gene05781	MS.gene08889	0.848288	6.74E-60	-1.69E-46
MS.gene057999	MS.gene08889	0.822958	1.79E-53	-1.69E-46
MS.gene058054	MS.gene08889	0.813413	2.59E-51	-1.69E-46
MS.gene058055	MS.gene08889	0.804928	1.71E-49	-1.69E-46
MS.gene058261	MS.gene08889	0.83704	6.57E-57	-1.69E-46
MS.gene058278	MS.gene08889	0.838398	2.94E-57	-1.69E-46
MS.gene058438	MS.gene08889	0.809515	1.82E-50	-1.69E-46
MS.gene058638	MS.gene08889	0.813819	2.11E-51	-1.69E-46
MS.gene058639	MS.gene08889	0.817512	3.17E-52	-1.69E-46
MS.gene058646	MS.gene08889	0.839421	1.60E-57	-1.69E-46
MS.gene058654	MS.gene08889	0.836586	8.58E-57	-1.69E-46
MS.gene058758	MS.gene08889	0.834646	2.66E-56	-1.69E-46
MS.gene059007	MS.gene08889	0.824882	6.34E-54	-1.69E-46
MS.gene059146	MS.gene08889	0.802965	4.37E-49	-1.69E-46
MS.gene059171	MS.gene08889	0.813896	2.03E-51	-1.69E-46
MS.gene059290	MS.gene08889	0.807254	5.53E-50	-1.69E-46
MS.gene059360	MS.gene08889	0.808817	2.57E-50	-1.69E-46
MS.gene05978	MS.gene08889	0.801782	7.65E-49	-1.69E-46
MS.gene059872	MS.gene08889	-0.80633	8.67E-50	-1.69E-46
MS.gene060084	MS.gene08889	0.803911	2.78E-49	-1.69E-46
MS.gene06010	MS.gene08889	0.816536	5.26E-52	-1.69E-46
MS.gene060296	MS.gene08889	0.820306	7.35E-53	-1.69E-46
MS.gene060465	MS.gene08889	0.800417	1.46E-48	-1.69E-46
MS.gene060563	MS.gene08889	0.833775	4.39E-56	-1.69E-46
MS.gene060869	MS.gene08889	0.816358	5.76E-52	-1.69E-46
MS.gene060870	MS.gene08889	0.815006	1.15E-51	-1.69E-46
MS.gene060910	MS.gene08889	0.800486	1.41E-48	-1.69E-46
MS.gene060932	MS.gene08889	0.807412	5.12E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08889.t1	MTR_8g080000	97.183	71	2	0	1	71	96	166	1.07e-46	155

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08889.t1	AT3G26000	55.172	58	22	2	1	54	57	114	2.15e-13	63.2

Find 19 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTGGATGGCTAGAGGATT+TGG	0.233006	4.4:+16689966	MS.gene08889:CDS
GAGAATTACCCTTTGTTTGC+TGG	0.274541	4.4:+16690039	MS.gene08889:CDS
TCATTTGGAATAATGCTGTT+AGG	0.357009	4.4:+16690070	MS.gene08889:CDS
TTGCTGGGTTGAATTTCATT+TGG	0.372245	4.4:+16690055	MS.gene08889:CDS
TATCATGTAAAGGATGAGAT+TGG	0.410235	4.4:+16690006	MS.gene08889:CDS
AGAATTACCCTTTGTTTGCT+GGG	0.418876	4.4:+16690040	MS.gene08889:CDS
ATCATGTAAAGGATGAGATT+GGG	0.506445	4.4:+16690007	MS.gene08889:CDS
CATTGAAGAGAAGGATTTCG+TGG	0.521110	4.4:+16690125	MS.gene08889:CDS
ACGACGTTCACAGCCATCAC+TGG	0.522090	4.4:+16689949	MS.gene08889:CDS
GAATGTGTGCATTGAAGAGA+AGG	0.529253	4.4:+16690116	MS.gene08889:CDS
AATTCAACCCAGCAAACAAA+GGG	0.559439	4.4:-16690047	None:intergenic
ATCCTCTAGCCATCCAGTGA+TGG	0.576800	4.4:-16689962	None:intergenic
ATTGAAGAGAAGGATTTCGT+GGG	0.604718	4.4:+16690126	MS.gene08889:CDS
AAATTCAACCCAGCAAACAA+AGG	0.606843	4.4:-16690048	None:intergenic
CGTTCACAGCCATCACTGGA+TGG	0.612700	4.4:+16689953	MS.gene08889:CDS
AGCCATCACTGGATGGCTAG+AGG	0.621316	4.4:+16689960	MS.gene08889:CDS
TTATGGTGGATATCATGTAA+AGG	0.645973	4.4:+16689996	MS.gene08889:CDS
AAGGATGAGATTGGGACGAG+TGG	0.669294	4.4:+16690015	MS.gene08889:CDS
TTGAAGAGAAGGATTTCGTG+GGG	0.689003	4.4:+16690127	MS.gene08889:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCATGTAAAGGATGAGATT+GGG	+	chr4.4:16690007-16690026	MS.gene08889:CDS	30.0%
	TATCATGTAAAGGATGAGAT+TGG	+	chr4.4:16690006-16690025	MS.gene08889:CDS	30.0%
!	TCATTTGGAATAATGCTGTT+AGG	+	chr4.4:16690070-16690089	MS.gene08889:CDS	30.0%
!!	TTATGGTGGATATCATGTAA+AGG	+	chr4.4:16689996-16690015	MS.gene08889:CDS	30.0%
!!!	GAGGATTTGGATTTTGATTA+TGG	+	chr4.4:16689979-16689998	MS.gene08889:CDS	30.0%
!!!	GATTTGGATTTTGATTATGG+TGG	+	chr4.4:16689982-16690001	MS.gene08889:CDS	30.0%
	AAATTCAACCCAGCAAACAA+AGG	-	chr4.4:16690051-16690070	None:intergenic	35.0%
	AATTCAACCCAGCAAACAAA+GGG	-	chr4.4:16690050-16690069	None:intergenic	35.0%
	ATTGAAGAGAAGGATTTCGT+GGG	+	chr4.4:16690126-16690145	MS.gene08889:CDS	35.0%
!	AGAATTACCCTTTGTTTGCT+GGG	+	chr4.4:16690040-16690059	MS.gene08889:CDS	35.0%
!	TTGCTGGGTTGAATTTCATT+TGG	+	chr4.4:16690055-16690074	MS.gene08889:CDS	35.0%
	CATTGAAGAGAAGGATTTCG+TGG	+	chr4.4:16690125-16690144	MS.gene08889:CDS	40.0%
	GAATGTGTGCATTGAAGAGA+AGG	+	chr4.4:16690116-16690135	MS.gene08889:CDS	40.0%
	TTGAAGAGAAGGATTTCGTG+GGG	+	chr4.4:16690127-16690146	MS.gene08889:CDS	40.0%
!	GAGAATTACCCTTTGTTTGC+TGG	+	chr4.4:16690039-16690058	MS.gene08889:CDS	40.0%
!	AGGTTCCACACTTTTCCTCA+AGG	+	chr4.4:16690090-16690109	MS.gene08889:CDS	45.0%
!	GGTTCCACACTTTTCCTCAA+GGG	+	chr4.4:16690091-16690110	MS.gene08889:CDS	45.0%
!	GTTCCACACTTTTCCTCAAG+GGG	+	chr4.4:16690092-16690111	MS.gene08889:CDS	45.0%
!	TTCCACACTTTTCCTCAAGG+GGG	+	chr4.4:16690093-16690112	MS.gene08889:CDS	45.0%
	AAGGATGAGATTGGGACGAG+TGG	+	chr4.4:16690015-16690034	MS.gene08889:CDS	50.0%
	ATCCTCTAGCCATCCAGTGA+TGG	-	chr4.4:16689965-16689984	None:intergenic	50.0%
	CACTGGATGGCTAGAGGATT+TGG	+	chr4.4:16689966-16689985	MS.gene08889:CDS	50.0%
!	TCCACACTTTTCCTCAAGGG+GGG	+	chr4.4:16690094-16690113	MS.gene08889:CDS	50.0%
	ACGACGTTCACAGCCATCAC+TGG	+	chr4.4:16689949-16689968	MS.gene08889:CDS	55.0%
	AGCCATCACTGGATGGCTAG+AGG	+	chr4.4:16689960-16689979	MS.gene08889:CDS	55.0%
	ATGCACACATTCCCCCCTTG+AGG	-	chr4.4:16690108-16690127	None:intergenic	55.0%
	CGTTCACAGCCATCACTGGA+TGG	+	chr4.4:16689953-16689972	MS.gene08889:CDS	55.0%
	TCCCCCCTTGAGGAAAAGTG+TGG	-	chr4.4:16690098-16690117	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	16689937	16690152	16689937	ID=MS.gene08889
chr4.4	mRNA	16689937	16690152	16689937	ID=MS.gene08889.t1;Parent=MS.gene08889
chr4.4	exon	16689937	16690152	16689937	ID=MS.gene08889.t1.exon1;Parent=MS.gene08889.t1
chr4.4	CDS	16689937	16690152	16689937	ID=cds.MS.gene08889.t1;Parent=MS.gene08889.t1

Gene Sequence	>MS.gene08889 ATGGATATCAGTACGACGTTCACAGCCATCACTGGATGGCTAGAGGATTTGGATTTTGATTATGGTGGATATCATGTAAAGGATGAGATTGGGACGAGTGGTGAGAATTACCCTTTGTTTGCTGGGTTGAATTTCATTTGGAATAATGCTGTTAGGTTCCACACTTTTCCTCAAGGGGGGAATGTGTGCATTGAAGAGAAGGATTTCGTGGGGTGA
Protein sequence	>MS.gene08889.t1 MDISTTFTAITGWLEDLDFDYGGYHVKDEIGTSGENYPLFAGLNFIWNNAVRFHTFPQGGNVCIEEKDFVG