Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67107.t1 | XP_003589221.1 | 97.5 | 280 | 7 | 0 | 4 | 283 | 60 | 339 | 5.40E-157 | 563.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67107.t1 | G7I4F5 | 97.5 | 280 | 7 | 0 | 4 | 283 | 60 | 339 | 3.9e-157 | 563.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene67107 | 0.823577 | 1.29E-53 | -1.69E-46 |
| MS.gene049166 | MS.gene67107 | 0.856467 | 3.15E-62 | -1.69E-46 |
| MS.gene049178 | MS.gene67107 | 0.813618 | 2.34E-51 | -1.69E-46 |
| MS.gene04917 | MS.gene67107 | 0.823542 | 1.31E-53 | -1.69E-46 |
| MS.gene049208 | MS.gene67107 | 0.828167 | 1.04E-54 | -1.69E-46 |
| MS.gene049674 | MS.gene67107 | 0.803196 | 3.91E-49 | -1.69E-46 |
| MS.gene049756 | MS.gene67107 | 0.812559 | 3.99E-51 | -1.69E-46 |
| MS.gene049786 | MS.gene67107 | 0.828437 | 9.00E-55 | -1.69E-46 |
| MS.gene049822 | MS.gene67107 | 0.807509 | 4.88E-50 | -1.69E-46 |
| MS.gene04984 | MS.gene67107 | 0.823415 | 1.40E-53 | -1.69E-46 |
| MS.gene049887 | MS.gene67107 | 0.834153 | 3.53E-56 | -1.69E-46 |
| MS.gene050085 | MS.gene67107 | 0.801037 | 1.09E-48 | -1.69E-46 |
| MS.gene050114 | MS.gene67107 | 0.866673 | 2.40E-65 | -1.69E-46 |
| MS.gene050317 | MS.gene67107 | 0.861724 | 8.39E-64 | -1.69E-46 |
| MS.gene050439 | MS.gene67107 | 0.822747 | 2.01E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene67107 | 0.802022 | 6.83E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene67107 | 0.840505 | 8.35E-58 | -1.69E-46 |
| MS.gene051003 | MS.gene67107 | 0.828514 | 8.62E-55 | -1.69E-46 |
| MS.gene051184 | MS.gene67107 | 0.813113 | 3.02E-51 | -1.69E-46 |
| MS.gene051245 | MS.gene67107 | 0.824086 | 9.76E-54 | -1.69E-46 |
| MS.gene051746 | MS.gene67107 | 0.826967 | 2.03E-54 | -1.69E-46 |
| MS.gene051748 | MS.gene67107 | 0.85686 | 2.42E-62 | -1.69E-46 |
| MS.gene051756 | MS.gene67107 | 0.838097 | 3.52E-57 | -1.69E-46 |
| MS.gene051757 | MS.gene67107 | 0.815293 | 9.96E-52 | -1.69E-46 |
| MS.gene051852 | MS.gene67107 | 0.810388 | 1.18E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene67107 | 0.808085 | 3.68E-50 | -1.69E-46 |
| MS.gene052072 | MS.gene67107 | 0.800261 | 1.57E-48 | -1.69E-46 |
| MS.gene052307 | MS.gene67107 | 0.809802 | 1.58E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene67107 | 0.813649 | 2.30E-51 | -1.69E-46 |
| MS.gene052434 | MS.gene67107 | 0.853302 | 2.61E-61 | -1.69E-46 |
| MS.gene052527 | MS.gene67107 | 0.825496 | 4.54E-54 | -1.69E-46 |
| MS.gene053058 | MS.gene67107 | 0.812349 | 4.43E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene67107 | 0.825101 | 5.63E-54 | -1.69E-46 |
| MS.gene053167 | MS.gene67107 | 0.811071 | 8.41E-51 | -1.69E-46 |
| MS.gene053381 | MS.gene67107 | 0.822899 | 1.85E-53 | -1.69E-46 |
| MS.gene053677 | MS.gene67107 | 0.812164 | 4.87E-51 | -1.69E-46 |
| MS.gene05380 | MS.gene67107 | 0.812396 | 4.33E-51 | -1.69E-46 |
| MS.gene054823 | MS.gene67107 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene67107 | 0.858686 | 6.95E-63 | -1.69E-46 |
| MS.gene055313 | MS.gene67107 | 0.83434 | 3.17E-56 | -1.69E-46 |
| MS.gene055314 | MS.gene67107 | 0.8357 | 1.44E-56 | -1.69E-46 |
| MS.gene055441 | MS.gene67107 | 0.823074 | 1.68E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene67107 | 0.807717 | 4.41E-50 | -1.69E-46 |
| MS.gene055603 | MS.gene67107 | 0.812461 | 4.19E-51 | -1.69E-46 |
| MS.gene056097 | MS.gene67107 | 0.80061 | 1.33E-48 | -1.69E-46 |
| MS.gene056181 | MS.gene67107 | 0.857214 | 1.90E-62 | -1.69E-46 |
| MS.gene056403 | MS.gene67107 | 0.800752 | 1.24E-48 | -1.69E-46 |
| MS.gene056432 | MS.gene67107 | 0.823141 | 1.62E-53 | -1.69E-46 |
| MS.gene056638 | MS.gene67107 | 0.804757 | 1.85E-49 | -1.69E-46 |
| MS.gene056752 | MS.gene67107 | 0.83677 | 7.70E-57 | -1.69E-46 |
| MS.gene056787 | MS.gene67107 | 0.838587 | 2.63E-57 | -1.69E-46 |
| MS.gene056849 | MS.gene67107 | 0.802989 | 4.32E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene67107 | 0.814404 | 1.57E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene67107 | 0.847729 | 9.62E-60 | -1.69E-46 |
| MS.gene057094 | MS.gene67107 | 0.826975 | 2.02E-54 | -1.69E-46 |
| MS.gene057140 | MS.gene67107 | 0.811662 | 6.26E-51 | -1.69E-46 |
| MS.gene057234 | MS.gene67107 | 0.813068 | 3.09E-51 | -1.69E-46 |
| MS.gene057360 | MS.gene67107 | 0.817716 | 2.85E-52 | -1.69E-46 |
| MS.gene057452 | MS.gene67107 | -0.823836 | 1.12E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene67107 | 0.803411 | 3.53E-49 | -1.69E-46 |
| MS.gene058055 | MS.gene67107 | 0.802432 | 5.63E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene67107 | 0.818404 | 2.00E-52 | -1.69E-46 |
| MS.gene058438 | MS.gene67107 | 0.808643 | 2.80E-50 | -1.69E-46 |
| MS.gene058638 | MS.gene67107 | 0.829151 | 6.04E-55 | -1.69E-46 |
| MS.gene058639 | MS.gene67107 | 0.847246 | 1.31E-59 | -1.69E-46 |
| MS.gene058646 | MS.gene67107 | 0.820031 | 8.50E-53 | -1.69E-46 |
| MS.gene058654 | MS.gene67107 | 0.845379 | 4.21E-59 | -1.69E-46 |
| MS.gene058758 | MS.gene67107 | 0.855387 | 6.53E-62 | -1.69E-46 |
| MS.gene05880 | MS.gene67107 | 0.817977 | 2.49E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene67107 | 0.815411 | 9.37E-52 | -1.69E-46 |
| MS.gene059050 | MS.gene67107 | 0.812036 | 5.19E-51 | -1.69E-46 |
| MS.gene059171 | MS.gene67107 | 0.85548 | 6.13E-62 | -1.69E-46 |
| MS.gene059360 | MS.gene67107 | 0.808224 | 3.44E-50 | -1.69E-46 |
| MS.gene059719 | MS.gene67107 | 0.824942 | 6.14E-54 | -1.69E-46 |
| MS.gene06010 | MS.gene67107 | 0.841616 | 4.26E-58 | -1.69E-46 |
| MS.gene06011 | MS.gene67107 | 0.845104 | 5.00E-59 | -1.69E-46 |
| MS.gene060296 | MS.gene67107 | 0.809643 | 1.71E-50 | -1.69E-46 |
| MS.gene060465 | MS.gene67107 | 0.805598 | 1.24E-49 | -1.69E-46 |
| MS.gene060563 | MS.gene67107 | 0.824811 | 6.59E-54 | -1.69E-46 |
| MS.gene060637 | MS.gene67107 | 0.819749 | 9.86E-53 | -1.69E-46 |
| MS.gene060702 | MS.gene67107 | 0.811233 | 7.75E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene67107 | 0.817242 | 3.65E-52 | -1.69E-46 |
| MS.gene060869 | MS.gene67107 | 0.872439 | 3.18E-67 | -1.69E-46 |
| MS.gene060870 | MS.gene67107 | 0.863848 | 1.86E-64 | -1.69E-46 |
| MS.gene060910 | MS.gene67107 | 0.800899 | 1.16E-48 | -1.69E-46 |
| MS.gene061006 | MS.gene67107 | 0.804539 | 2.06E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67107.t1 | MTR_1g019780 | 97.500 | 280 | 7 | 0 | 4 | 283 | 60 | 339 | 0.0 | 565 |
| MS.gene67107.t1 | MTR_3g101480 | 70.992 | 262 | 70 | 1 | 9 | 270 | 54 | 309 | 5.87e-138 | 392 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67107.t1 | AT4G33380 | 62.278 | 281 | 97 | 3 | 4 | 283 | 56 | 328 | 5.28e-121 | 349 |
| MS.gene67107.t1 | AT4G33380 | 61.566 | 281 | 97 | 4 | 4 | 283 | 56 | 326 | 9.78e-118 | 341 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCAGCTGCACTATCTTTA+TGG | 0.210691 | 1.3:+3830149 | None:intergenic |
| ACCAGATCAGATTCGGAAAA+TGG | 0.226467 | 1.3:-3837264 | MS.gene67107:CDS |
| GTTTACTGTCCGTCAGAATT+TGG | 0.251133 | 1.3:-3830061 | MS.gene67107:CDS |
| ACCGGTGAAGTTAATGAAAT+TGG | 0.259283 | 1.3:-3837210 | MS.gene67107:intron |
| ATACAGATGCAGTTGAAAAT+TGG | 0.267336 | 1.3:-3830815 | MS.gene67107:intron |
| CCTGATGGTGCAGAGGATTT+AGG | 0.291489 | 1.3:-3830447 | MS.gene67107:intron |
| CCAGAGGAGGAAGTTCTTCC+AGG | 0.300552 | 1.3:-3830250 | MS.gene67107:intron |
| TGGAAGAATCGAATCGAATT+AGG | 0.327853 | 1.3:-3837244 | MS.gene67107:CDS |
| ATGACGGTTCTGCGGTTAGA+TGG | 0.328426 | 1.3:-3830183 | MS.gene67107:CDS |
| TTGAATAGCAGAAGCAATAA+TGG | 0.346033 | 1.3:-3836995 | MS.gene67107:CDS |
| CTATCCTTAAAATTTAGTTT+TGG | 0.353734 | 1.3:+3829352 | None:intergenic |
| GCTGTGCTTGCTGAAGAAAT+TGG | 0.359440 | 1.3:-3830516 | MS.gene67107:CDS |
| TCATAATCTGTATGAAGTTC+AGG | 0.363566 | 1.3:+3830202 | None:intergenic |
| AGGGATGCTAGTTAAAGCTT+TGG | 0.401229 | 1.3:-3830550 | MS.gene67107:CDS |
| TAAAGCTTTGGAGTCTGATT+GGG | 0.405512 | 1.3:-3830538 | MS.gene67107:CDS |
| TTAAAGCTTTGGAGTCTGAT+TGG | 0.415568 | 1.3:-3830539 | MS.gene67107:CDS |
| TGTGAATTTCGATACCATTC+GGG | 0.422775 | 1.3:+3829325 | None:intergenic |
| ATGTGAATTTCGATACCATT+CGG | 0.427345 | 1.3:+3829324 | None:intergenic |
| GTGATTGTTCCATGGGCCTC+TGG | 0.427846 | 1.3:-3829289 | MS.gene67107:CDS |
| TGATTGCTGTCAAGCTTGCT+TGG | 0.436458 | 1.3:-3830131 | MS.gene67107:CDS |
| TGTTTGTGCTGATAAATATC+TGG | 0.438090 | 1.3:+3830028 | None:intergenic |
| ACCAATTTCATTAACTTCAC+CGG | 0.438559 | 1.3:+3837209 | None:intergenic |
| TGACGGTTCTGCGGTTAGAT+GGG | 0.442080 | 1.3:-3830182 | MS.gene67107:CDS |
| TCAGGTGGAAGGGCCCTGCC+TGG | 0.445771 | 1.3:+3830232 | None:intergenic |
| CCTAAATCCTCTGCACCATC+AGG | 0.447349 | 1.3:+3830447 | None:intergenic |
| TCTACATTCACAACCTCAAC+AGG | 0.453666 | 1.3:+3830483 | None:intergenic |
| CTGCACCAGTGATTGTTCCA+TGG | 0.458082 | 1.3:-3829297 | MS.gene67107:CDS |
| CTCGCTCCATAGCAATGCCT+TGG | 0.461557 | 1.3:+3836963 | None:intergenic |
| TATTTATCAGCACAAACATA+TGG | 0.462668 | 1.3:-3830023 | MS.gene67107:CDS |
| TTGCTGAAGAAATTGGCCTT+TGG | 0.466078 | 1.3:-3830509 | MS.gene67107:CDS |
| TGCACCAGTGATTGTTCCAT+GGG | 0.469487 | 1.3:-3829296 | MS.gene67107:CDS |
| GCAACTCAACCTCCGTACTT+TGG | 0.472261 | 1.3:+3837071 | None:intergenic |
| AGGAGGAAGTTCTTCCAGGC+AGG | 0.474177 | 1.3:-3830246 | MS.gene67107:CDS |
| TTCTTGAGAAACGGTAGAGT+TGG | 0.476728 | 1.3:+3830743 | None:intergenic |
| GAAGTTAAATTAGTTTCTGC+GGG | 0.485113 | 1.3:-3830774 | MS.gene67107:CDS |
| AAAAGGTGAAGGAATTAGAA+GGG | 0.491684 | 1.3:-3837105 | MS.gene67107:intron |
| TGTATAAACCAGATCAGATT+CGG | 0.492887 | 1.3:-3837271 | MS.gene67107:CDS |
| ACCTGCCTCTTCTTGAGAAA+CGG | 0.495444 | 1.3:+3830734 | None:intergenic |
| CAGGTTTGCACTCAGGTGGA+AGG | 0.507192 | 1.3:+3830221 | None:intergenic |
| TCGCTCCATAGCAATGCCTT+GGG | 0.512530 | 1.3:+3836964 | None:intergenic |
| AGCACAAACATATGGAATGC+TGG | 0.516650 | 1.3:-3830015 | MS.gene67107:CDS |
| AGGTTTGCACTCAGGTGGAA+GGG | 0.517485 | 1.3:+3830222 | None:intergenic |
| TGAAGTTCAGGTTTGCACTC+AGG | 0.518943 | 1.3:+3830214 | None:intergenic |
| CTTCTTTGGCACGTTTAGCA+TGG | 0.526328 | 1.3:+3830107 | None:intergenic |
| CATGCTAAACGTGCCAAAGA+AGG | 0.528974 | 1.3:-3830106 | MS.gene67107:CDS |
| AATTAGGAAGTCATACAAAC+CGG | 0.530432 | 1.3:-3837228 | MS.gene67107:CDS |
| CTCTACCGTTTCTCAAGAAG+AGG | 0.531814 | 1.3:-3830739 | MS.gene67107:CDS |
| ATCTTCTGTTTCAAGTGCTG+AGG | 0.533874 | 1.3:+3837049 | None:intergenic |
| ACCGTTTCTCAAGAAGAGGC+AGG | 0.537821 | 1.3:-3830735 | MS.gene67107:intron |
| GGAACAATCACTGGTGCAGA+TGG | 0.546225 | 1.3:+3829301 | None:intergenic |
| GAGGCCCATGGAACAATCAC+TGG | 0.549857 | 1.3:+3829292 | None:intergenic |
| AGAAGTTAAATTAGTTTCTG+CGG | 0.551102 | 1.3:-3830775 | MS.gene67107:CDS |
| TTAAATTAGTTTCTGCGGGA+GGG | 0.559450 | 1.3:-3830770 | MS.gene67107:CDS |
| TCACTCCCAAGGCATTGCTA+TGG | 0.562744 | 1.3:-3836969 | MS.gene67107:CDS |
| GACGGTTCTGCGGTTAGATG+GGG | 0.565108 | 1.3:-3830181 | MS.gene67107:CDS |
| AGTTCAGGTTTGCACTCAGG+TGG | 0.566503 | 1.3:+3830217 | None:intergenic |
| GGAGGAAGTTCTTCCAGGCA+GGG | 0.592808 | 1.3:-3830245 | MS.gene67107:CDS |
| TGCAGTTGAAAATTGGCGCA+AGG | 0.598206 | 1.3:-3830808 | MS.gene67107:CDS |
| GTTAAATTAGTTTCTGCGGG+AGG | 0.608057 | 1.3:-3830771 | MS.gene67107:CDS |
| TGGCCTTTGGATACCTGTTG+AGG | 0.611467 | 1.3:-3830496 | MS.gene67107:CDS |
| GAACTTCATACAGATTATGA+CGG | 0.619220 | 1.3:-3830199 | MS.gene67107:CDS |
| GAATGACATCCAAATTCTGA+CGG | 0.620048 | 1.3:+3830052 | None:intergenic |
| AAAGGTGAAGGAATTAGAAG+GGG | 0.620122 | 1.3:-3837104 | MS.gene67107:intron |
| GCAACAACTCCAGAGGCCCA+TGG | 0.623742 | 1.3:+3829280 | None:intergenic |
| CAAGGCATTGCTATGGAGCG+AGG | 0.630111 | 1.3:-3836962 | MS.gene67107:intron |
| TGACAAACCTGATGGTGCAG+AGG | 0.630542 | 1.3:-3830454 | MS.gene67107:CDS |
| AATAATGGATCTCACTCCCA+AGG | 0.631172 | 1.3:-3836980 | MS.gene67107:CDS |
| TACAGATTATGACGGTTCTG+CGG | 0.638373 | 1.3:-3830191 | MS.gene67107:CDS |
| GAAACAGAAGATAATTGATG+AGG | 0.645919 | 1.3:-3837038 | MS.gene67107:CDS |
| TTAAGGATAGGTATCCCGAA+TGG | 0.659919 | 1.3:-3829339 | MS.gene67107:CDS |
| CAACCTCAACAGGTATCCAA+AGG | 0.662747 | 1.3:+3830493 | None:intergenic |
| TCAAGCAGCAACAACTCCAG+AGG | 0.687351 | 1.3:+3829273 | None:intergenic |
| GAACATAATGACAAACCTGA+TGG | 0.690687 | 1.3:-3830462 | MS.gene67107:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 3829273 | 3837708 | 3829273 | ID=MS.gene67107 |
| chr1.3 | mRNA | 3829273 | 3837708 | 3829273 | ID=MS.gene67107.t1;Parent=MS.gene67107 |
| chr1.3 | exon | 3837699 | 3837708 | 3837699 | ID=MS.gene67107.t1.exon1;Parent=MS.gene67107.t1 |
| chr1.3 | CDS | 3837699 | 3837708 | 3837699 | ID=cds.MS.gene67107.t1;Parent=MS.gene67107.t1 |
| chr1.3 | exon | 3837211 | 3837299 | 3837211 | ID=MS.gene67107.t1.exon2;Parent=MS.gene67107.t1 |
| chr1.3 | CDS | 3837211 | 3837299 | 3837211 | ID=cds.MS.gene67107.t1;Parent=MS.gene67107.t1 |
| chr1.3 | exon | 3836963 | 3837122 | 3836963 | ID=MS.gene67107.t1.exon3;Parent=MS.gene67107.t1 |
| chr1.3 | CDS | 3836963 | 3837122 | 3836963 | ID=cds.MS.gene67107.t1;Parent=MS.gene67107.t1 |
| chr1.3 | exon | 3830736 | 3830831 | 3830736 | ID=MS.gene67107.t1.exon4;Parent=MS.gene67107.t1 |
| chr1.3 | CDS | 3830736 | 3830831 | 3830736 | ID=cds.MS.gene67107.t1;Parent=MS.gene67107.t1 |
| chr1.3 | exon | 3830448 | 3830570 | 3830448 | ID=MS.gene67107.t1.exon5;Parent=MS.gene67107.t1 |
| chr1.3 | CDS | 3830448 | 3830570 | 3830448 | ID=cds.MS.gene67107.t1;Parent=MS.gene67107.t1 |
| chr1.3 | exon | 3830006 | 3830268 | 3830006 | ID=MS.gene67107.t1.exon6;Parent=MS.gene67107.t1 |
| chr1.3 | CDS | 3830006 | 3830268 | 3830006 | ID=cds.MS.gene67107.t1;Parent=MS.gene67107.t1 |
| chr1.3 | exon | 3829273 | 3829383 | 3829273 | ID=MS.gene67107.t1.exon7;Parent=MS.gene67107.t1 |
| chr1.3 | CDS | 3829273 | 3829383 | 3829273 | ID=cds.MS.gene67107.t1;Parent=MS.gene67107.t1 |
| Gene Sequence |
| Protein sequence |
