AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06857


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06857.t1 MTR_3g092150 97.959 245 5 0 1 245 1 245 1.27e-180 495
MS.gene06857.t1 MTR_8g026960 43.269 208 110 4 42 245 38 241 2.42e-47 157
MS.gene06857.t1 MTR_6g007647 45.185 135 63 2 7 130 25 159 3.64e-30 114
MS.gene06857.t1 MTR_6g007647 57.447 94 40 0 37 130 32 125 4.78e-30 112
MS.gene06857.t1 MTR_4g107650 56.122 98 43 0 37 134 94 191 4.98e-29 112
MS.gene06857.t1 MTR_6g011610 56.383 94 41 0 37 130 95 188 8.10e-29 110
MS.gene06857.t1 MTR_6g011610 56.383 94 41 0 37 130 76 169 8.20e-29 109
MS.gene06857.t1 MTR_8g468210 54.255 94 43 0 37 130 76 169 4.31e-28 108
MS.gene06857.t1 MTR_1g061660 49.533 107 46 2 37 142 58 157 1.32e-26 103
MS.gene06857.t1 MTR_5g038280 55.435 92 41 0 39 130 88 179 1.92e-26 105
MS.gene06857.t1 MTR_8g089895 55.435 92 41 0 39 130 55 146 2.55e-26 104
MS.gene06857.t1 MTR_5g039000 54.255 94 43 0 37 130 76 169 3.05e-26 103
MS.gene06857.t1 MTR_3g086790 51.064 94 46 0 37 130 59 152 6.48e-26 103
MS.gene06857.t1 MTR_7g103340 41.304 138 67 3 37 160 62 199 4.07e-25 99.4
MS.gene06857.t1 MTR_8g469430 54.348 92 42 0 39 130 90 181 5.54e-25 100
MS.gene06857.t1 MTR_3g080100 49.474 95 48 0 36 130 18 112 6.29e-25 98.6
MS.gene06857.t1 MTR_3g080100 49.474 95 48 0 36 130 57 151 1.04e-24 99.0
MS.gene06857.t1 MTR_7g010020 47.826 92 48 0 39 130 81 172 3.15e-21 90.1
MS.gene06857.t1 MTR_5g019680 47.368 114 46 2 31 130 39 152 5.08e-21 87.8
MS.gene06857.t1 MTR_5g019650 52.577 97 38 2 31 120 39 134 2.00e-19 84.0
MS.gene06857.t1 MTR_4g100550 44.211 95 46 1 39 133 121 208 9.94e-14 69.3
MS.gene06857.t1 MTR_7g093430 41.346 104 48 2 33 133 155 248 1.03e-12 66.6
MS.gene06857.t1 MTR_4g126900 42.857 84 48 0 41 124 91 174 1.90e-12 65.1
MS.gene06857.t1 MTR_4g097600 43.158 95 47 1 39 133 128 215 2.94e-12 65.1
MS.gene06857.t1 MTR_5g014890 43.750 96 53 1 39 133 124 219 5.38e-12 64.3
MS.gene06857.t1 MTR_1g017090 43.158 95 47 1 39 133 131 218 8.48e-12 63.9
MS.gene06857.t1 MTR_1g054285 39.423 104 56 1 33 136 149 245 1.51e-11 63.2
MS.gene06857.t1 MTR_3g103590 45.333 75 41 0 36 110 143 217 1.69e-11 63.2
MS.gene06857.t1 MTR_8g006705 42.105 95 48 1 39 133 147 234 2.10e-11 63.2
MS.gene06857.t1 MTR_7g027265 31.250 144 73 4 35 177 17 135 2.42e-11 60.5
MS.gene06857.t1 MTR_2g061030 40.000 95 50 1 39 133 201 288 7.96e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06857.t1 AT2G46680 41.909 241 119 5 25 245 19 258 1.13e-44 150
MS.gene06857.t1 AT2G46680 42.259 239 119 5 25 245 19 256 1.43e-43 148
MS.gene06857.t1 AT3G61890 68.132 91 29 0 41 131 33 123 2.11e-38 134
MS.gene06857.t1 AT3G01470 40.972 144 85 0 37 180 67 210 7.27e-30 112
MS.gene06857.t1 AT2G22430 56.383 94 41 0 37 130 61 154 4.62e-29 111
MS.gene06857.t1 AT4G40060 52.041 98 47 0 37 134 58 155 2.16e-28 109
MS.gene06857.t1 AT1G69780 54.255 94 43 0 37 130 84 177 9.39e-28 107
MS.gene06857.t1 AT5G15150 53.191 94 44 0 37 130 114 207 3.82e-26 103
MS.gene06857.t1 AT2G18550 50.943 106 45 1 39 137 62 167 7.28e-26 101
MS.gene06857.t1 AT5G65310 51.064 94 46 0 37 130 53 146 2.98e-25 101
MS.gene06857.t1 AT5G65310 51.064 94 46 0 37 130 71 164 3.89e-25 101
MS.gene06857.t1 AT4G36740 42.647 136 61 2 17 135 25 160 8.86e-25 98.2
MS.gene06857.t1 AT4G36740 50.962 104 44 1 39 135 56 159 1.18e-24 97.8
MS.gene06857.t1 AT5G66700 51.000 100 42 1 39 131 72 171 1.28e-24 98.2
MS.gene06857.t1 AT3G01220 48.936 94 48 0 37 130 86 179 7.79e-24 97.4
MS.gene06857.t1 AT5G03790 43.478 115 54 2 20 134 63 166 1.66e-23 95.1
MS.gene06857.t1 AT1G26960 42.969 128 65 2 11 130 36 163 1.81e-22 92.8
MS.gene06857.t1 AT5G03790 46.000 100 50 1 20 119 63 158 6.99e-22 90.1
MS.gene06857.t1 AT1G27045 40.299 134 74 2 9 138 35 166 4.44e-21 89.4
MS.gene06857.t1 AT1G27045 45.361 97 53 0 34 130 21 117 6.31e-20 84.7
MS.gene06857.t1 AT1G27045 45.361 97 53 0 34 130 21 117 6.31e-20 84.7
MS.gene06857.t1 AT1G27045 39.683 126 72 1 9 130 35 160 7.33e-20 85.5
MS.gene06857.t1 AT1G27045 45.361 97 53 0 34 130 13 109 8.17e-20 84.0
MS.gene06857.t1 AT2G36610 42.308 104 45 2 39 134 72 168 2.55e-19 82.8
MS.gene06857.t1 AT5G53980 39.604 101 57 1 29 129 6 102 1.36e-15 72.4
MS.gene06857.t1 AT2G44910 42.000 100 51 1 39 138 164 256 1.49e-12 66.6
MS.gene06857.t1 AT3G60390 42.000 100 51 1 39 138 163 255 3.81e-12 65.1
MS.gene06857.t1 AT2G22800 42.105 95 48 1 39 133 114 201 1.17e-11 63.5
MS.gene06857.t1 AT5G06710 41.837 98 50 1 33 130 185 275 1.41e-11 63.9
MS.gene06857.t1 AT4G16780 42.105 95 48 1 39 133 130 217 6.08e-11 61.6
MS.gene06857.t1 AT2G01430 42.105 95 48 1 39 133 140 227 8.14e-11 61.2
MS.gene06857.t1 AT4G37790 41.053 95 49 1 39 133 127 214 8.81e-11 60.8

Find 36 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCCTAGACAAGTTGCTATA+TGG 0.329458 3.1:-74160849 MS.gene06857:CDS
GCAAGTTGCATCTTCTTCCT+TGG 0.339735 3.1:+74160889 None:intergenic
AGCACAGAAGATGGTGGTTC+AGG 0.365057 3.1:-74160377 MS.gene06857:CDS
GGTTCAGAAGCTAAGTTATA+TGG 0.367405 3.1:-74160441 MS.gene06857:CDS
GAAGATGGTGGTTCAGGAAA+TGG 0.372815 3.1:-74160371 MS.gene06857:CDS
GAAATGGATACAACAATTGC+AGG 0.379643 3.1:-74160355 MS.gene06857:CDS
ATGCAACTTGCAAGAGATCT+TGG 0.385707 3.1:-74160877 MS.gene06857:CDS
TTCTAAGTTTCAGTGCCTTA+AGG 0.396105 3.1:-74160743 MS.gene06857:CDS
AAGACTCTTTCTCTTCCTTA+AGG 0.407960 3.1:+74160728 None:intergenic
TGATGAACAGATAAGATCAT+TGG 0.455417 3.1:-74160944 MS.gene06857:CDS
ATGCAATAGTTCTCAATGGT+TGG 0.475551 3.1:-74160117 MS.gene06857:CDS
AATATTCATGAAGGGTTCAA+AGG 0.493550 3.1:-74161054 MS.gene06857:CDS
GAAGAAGAGAATATTCATGA+AGG 0.503417 3.1:-74161063 MS.gene06857:CDS
CAGAATCAAAGTTGGAGCCA+AGG 0.518559 3.1:-74160906 MS.gene06857:CDS
ATTTGAGTCAGAATCAAAGT+TGG 0.518777 3.1:-74160914 MS.gene06857:CDS
AAGGAAAATAGCACAGAAGA+TGG 0.540540 3.1:-74160386 MS.gene06857:CDS
GAAAGAGTCTTTGCAATCAG+AGG 0.541327 3.1:-74160716 MS.gene06857:intron
AATCATGCAATAGTTCTCAA+TGG 0.553811 3.1:-74160121 MS.gene06857:CDS
AGAAGATAGAAAACAAGAGA+AGG 0.569555 3.1:-74160972 MS.gene06857:CDS
AAACATCGCATGATGGAGGA+AGG 0.574577 3.1:-74160415 MS.gene06857:CDS
AACCATATAGCAACTTGTCT+AGG 0.580258 3.1:+74160847 None:intergenic
AAGAAGAGAATATTCATGAA+GGG 0.590509 3.1:-74161062 MS.gene06857:CDS
TTCAGAACAGAAGAGCAAGA+TGG 0.602218 3.1:-74160825 MS.gene06857:CDS
TGATGGAGGAAGGGAAGCTA+AGG 0.606332 3.1:-74160405 MS.gene06857:CDS
TCAGAAGCTAAGTTATATGG+TGG 0.615960 3.1:-74160438 MS.gene06857:CDS
ATGGTGGAAACATCGCATGA+TGG 0.617224 3.1:-74160422 MS.gene06857:CDS
AACATCGCATGATGGAGGAA+GGG 0.628539 3.1:-74160414 MS.gene06857:CDS
GTGGAAACATCGCATGATGG+AGG 0.645127 3.1:-74160419 MS.gene06857:CDS
GGCCATATATAATATGATGG+AGG 0.645919 3.1:-74161085 None:intergenic
GAGCAAGATGGAAATCAAAG+AGG 0.650346 3.1:-74160813 MS.gene06857:CDS
GATTGATCTAACATACCACA+TGG 0.663576 3.1:+74160140 None:intergenic
GAAAATAGCACAGAAGATGG+TGG 0.663671 3.1:-74160383 MS.gene06857:CDS
AAAATGAAAGAAGCACAACA+TGG 0.668449 3.1:-74160262 MS.gene06857:CDS
ACAGAGAAAATTGAGGACAG+AGG 0.671559 3.1:-74160239 MS.gene06857:CDS
AACATGGACAGAGAAAATTG+AGG 0.710156 3.1:-74160246 MS.gene06857:CDS
TGGTACAATGTTGATCCATG+TGG 0.723453 3.1:-74160155 MS.gene06857:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAAGAGAATATTCATGAA+GGG - chr3.1:74160113-74160132 MS.gene06857:CDS 25.0%
!! AAAGCAAAGATTTTCAAATG+AGG - chr3.1:74160854-74160873 MS.gene06857:CDS 25.0%
!! AAGCAAAGATTTTCAAATGA+GGG - chr3.1:74160855-74160874 MS.gene06857:CDS 25.0%
!! TTTTTCTTCTTTGAATTCTG+AGG + chr3.1:74160181-74160200 None:intergenic 25.0%
AAGTTAATGTCGAGTCTATA+AGG + chr3.1:74160689-74160708 None:intergenic 30.0%
AATATTCATGAAGGGTTCAA+AGG - chr3.1:74160121-74160140 MS.gene06857:CDS 30.0%
AATCATGCAATAGTTCTCAA+TGG - chr3.1:74161054-74161073 MS.gene06857:CDS 30.0%
ACATACTCAAGCTTAGTATT+AGG - chr3.1:74160634-74160653 MS.gene06857:intron 30.0%
AGAAGATAGAAAACAAGAGA+AGG - chr3.1:74160203-74160222 MS.gene06857:CDS 30.0%
ATTTGAGTCAGAATCAAAGT+TGG - chr3.1:74160261-74160280 MS.gene06857:CDS 30.0%
GAAGAAGAGAATATTCATGA+AGG - chr3.1:74160112-74160131 MS.gene06857:CDS 30.0%
TGACAACAACACTTGAAAAA+TGG - chr3.1:74161000-74161019 MS.gene06857:CDS 30.0%
TGATGAACAGATAAGATCAT+TGG - chr3.1:74160231-74160250 MS.gene06857:CDS 30.0%
TGTTAGGAAACAAATTCACA+CGG + chr3.1:74160714-74160733 None:intergenic 30.0%
! AAAATGAAAGAAGCACAACA+TGG - chr3.1:74160913-74160932 MS.gene06857:CDS 30.0%
! AAGATTTTCAAATGAGGGTT+TGG - chr3.1:74160860-74160879 MS.gene06857:CDS 30.0%
!!! TTTTCAAGTGTTGTTGTCAA+GGG + chr3.1:74160999-74161018 None:intergenic 30.0%
!!! TTTTTCAAGTGTTGTTGTCA+AGG + chr3.1:74161000-74161019 None:intergenic 30.0%
AACATGGACAGAGAAAATTG+AGG - chr3.1:74160929-74160948 MS.gene06857:CDS 35.0%
AACCATATAGCAACTTGTCT+AGG + chr3.1:74160331-74160350 None:intergenic 35.0%
AAGACTCTTTCTCTTCCTTA+AGG + chr3.1:74160450-74160469 None:intergenic 35.0%
AAGGAAAATAGCACAGAAGA+TGG - chr3.1:74160789-74160808 MS.gene06857:CDS 35.0%
ATGCAATAGTTCTCAATGGT+TGG - chr3.1:74161058-74161077 MS.gene06857:CDS 35.0%
GAAATGGATACAACAATTGC+AGG - chr3.1:74160820-74160839 MS.gene06857:CDS 35.0%
GATTGATCTAACATACCACA+TGG + chr3.1:74161038-74161057 None:intergenic 35.0%
GGCTCGATTAATTAAATGAG+TGG - chr3.1:74160655-74160674 MS.gene06857:intron 35.0%
GGTTCAGAAGCTAAGTTATA+TGG - chr3.1:74160734-74160753 MS.gene06857:CDS 35.0%
TCAGAAGCTAAGTTATATGG+TGG - chr3.1:74160737-74160756 MS.gene06857:CDS 35.0%
TCAGAGGTAAGTTAGTAGTT+TGG - chr3.1:74160475-74160494 MS.gene06857:intron 35.0%
TTCTAAGTTTCAGTGCCTTA+AGG - chr3.1:74160432-74160451 MS.gene06857:CDS 35.0%
! ATTTTCAAATGAGGGTTTGG+AGG - chr3.1:74160863-74160882 MS.gene06857:CDS 35.0%
! GTGTGAATTTGTTTCCTAAC+AGG - chr3.1:74160713-74160732 MS.gene06857:intron 35.0%
! GTTCTCAATGGTTGGATTTT+TGG - chr3.1:74161066-74161085 MS.gene06857:CDS 35.0%
ACAGAGAAAATTGAGGACAG+AGG - chr3.1:74160936-74160955 MS.gene06857:CDS 40.0%
ACTTAGCTTCTGAACCTGTT+AGG + chr3.1:74160730-74160749 None:intergenic 40.0%
AGCCTAGACAAGTTGCTATA+TGG - chr3.1:74160326-74160345 MS.gene06857:CDS 40.0%
ATGCAACTTGCAAGAGATCT+TGG - chr3.1:74160298-74160317 MS.gene06857:CDS 40.0%
GAAAATAGCACAGAAGATGG+TGG - chr3.1:74160792-74160811 MS.gene06857:CDS 40.0%
GAAAGAGTCTTTGCAATCAG+AGG - chr3.1:74160459-74160478 MS.gene06857:intron 40.0%
GACTCAGCTCGTTAAATACT+CGG - chr3.1:74160585-74160604 MS.gene06857:intron 40.0%
GAGCAAGATGGAAATCAAAG+AGG - chr3.1:74160362-74160381 MS.gene06857:CDS 40.0%
TGGTACAATGTTGATCCATG+TGG - chr3.1:74161020-74161039 MS.gene06857:CDS 40.0%
TTCAGAACAGAAGAGCAAGA+TGG - chr3.1:74160350-74160369 MS.gene06857:CDS 40.0%
CAGAATCAAAGTTGGAGCCA+AGG - chr3.1:74160269-74160288 MS.gene06857:CDS 45.0%
GCAAGTTGCATCTTCTTCCT+TGG + chr3.1:74160289-74160308 None:intergenic 45.0%
! AAACATCGCATGATGGAGGA+AGG - chr3.1:74160760-74160779 MS.gene06857:CDS 45.0%
! AACATCGCATGATGGAGGAA+GGG - chr3.1:74160761-74160780 MS.gene06857:CDS 45.0%
! ATGGTGGAAACATCGCATGA+TGG - chr3.1:74160753-74160772 MS.gene06857:CDS 45.0%
! GAAGATGGTGGTTCAGGAAA+TGG - chr3.1:74160804-74160823 MS.gene06857:CDS 45.0%
GTGGAAACATCGCATGATGG+AGG - chr3.1:74160756-74160775 MS.gene06857:CDS 50.0%
TGATGGAGGAAGGGAAGCTA+AGG - chr3.1:74160770-74160789 MS.gene06857:CDS 50.0%
! AGCACAGAAGATGGTGGTTC+AGG - chr3.1:74160798-74160817 MS.gene06857:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 74160103 74161094 74160103 ID=MS.gene06857
chr3.1 mRNA 74160103 74161094 74160103 ID=MS.gene06857.t1;Parent=MS.gene06857
chr3.1 exon 74160717 74161094 74160717 ID=MS.gene06857.t1.exon1;Parent=MS.gene06857.t1
chr3.1 CDS 74160717 74161094 74160717 ID=cds.MS.gene06857.t1;Parent=MS.gene06857.t1
chr3.1 exon 74160103 74160462 74160103 ID=MS.gene06857.t1.exon2;Parent=MS.gene06857.t1
chr3.1 CDS 74160103 74160462 74160103 ID=cds.MS.gene06857.t1;Parent=MS.gene06857.t1
Gene Sequence

>MS.gene06857

ATGATGGAGGAAGAAGAGAATATTCATGAAGGGTTCAAAGGAAAAAATGTTGAAAGCATGAGTTGCTTAGAGACACCTCAGAATTCAAAGAAGAAAAACAAGAAGATAGAAAACAAGAGAAGGTTTAGTGATGAACAGATAAGATCATTGGAATGCATATTTGAGTCAGAATCAAAGTTGGAGCCAAGGAAGAAGATGCAACTTGCAAGAGATCTTGGTTTGCAGCCTAGACAAGTTGCTATATGGTTTCAGAACAGAAGAGCAAGATGGAAATCAAAGAGGATAGAACAAGAATACAGAAAACTCAAAGATGAATATGATAATTTAGCTTCTAAGTTTCAGTGCCTTAAGGAAGAGAAAGAGTCTTTGCAATCAGAGGTAAGTTAGTAGTTTGGTTCTTGTAATTGTAACATAACTAGAGTTGTGTCTCGATAAACAGCTTAATTTAATTTAAACGAGTTGAGCTATTCGTGAACTATTCAGACTCAGCTCGTTAAATACTCGGTTATGTTCGTTTGTTTAATTAAATAAACATACTCAAGCTTAGTATTAGGCTCGATTAATTAAATGAGTGGAACTTAAGCCTTATAGACTCGACATTAACTTAACCGTGTGAATTTGTTTCCTAACAGGTTCAGAAGCTAAGTTATATGGTGGAAACATCGCATGATGGAGGAAGGGAAGCTAAGGAAAATAGCACAGAAGATGGTGGTTCAGGAAATGGATACAACAATTGCAGGTTTGAAGAAACAAAGCAAAGATTTTCAAATGAGGGTTTGGAGGATAAAATCGTCATTTATTCAGATGATCAAAATGAAAGAAGCACAACATGGACAGAGAAAATTGAGGACAGAGGAAATCAACTTTTAAGAAATGATGATCATACAGAAATTCCCTTGACAACAACACTTGAAAAATGGTACAATGTTGATCCATGTGGTATGTTAGATCAATCATGCAATAGTTCTCAATGGTTGGATTTTTGGACTTGA

Protein sequence

>MS.gene06857.t1

MMEEEENIHEGFKGKNVESMSCLETPQNSKKKNKKIENKRRFSDEQIRSLECIFESESKLEPRKKMQLARDLGLQPRQVAIWFQNRRARWKSKRIEQEYRKLKDEYDNLASKFQCLKEEKESLQSEVQKLSYMVETSHDGGREAKENSTEDGGSGNGYNNCRFEETKQRFSNEGLEDKIVIYSDDQNERSTTWTEKIEDRGNQLLRNDDHTEIPLTTTLEKWYNVDPCGMLDQSCNSSQWLDFWT