AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06643


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06643.t1 MTR_3g092150 95.378 238 11 0 1 238 1 238 4.36e-158 439
MS.gene06643.t1 MTR_8g026960 44.865 185 94 4 42 222 38 218 6.60e-42 144
MS.gene06643.t1 MTR_6g007647 45.185 135 63 2 7 130 25 159 5.20e-30 114
MS.gene06643.t1 MTR_6g007647 57.447 94 40 0 37 130 32 125 6.13e-30 113
MS.gene06643.t1 MTR_4g107650 56.122 98 43 0 37 134 94 191 6.21e-29 112
MS.gene06643.t1 MTR_6g011610 56.383 94 41 0 37 130 76 169 1.86e-28 109
MS.gene06643.t1 MTR_6g011610 56.383 94 41 0 37 130 95 188 1.88e-28 109
MS.gene06643.t1 MTR_8g468210 54.255 94 43 0 37 130 76 169 7.11e-28 108
MS.gene06643.t1 MTR_1g061660 49.533 107 46 2 37 142 58 157 2.34e-26 103
MS.gene06643.t1 MTR_5g038280 55.435 92 41 0 39 130 88 179 2.85e-26 104
MS.gene06643.t1 MTR_8g089895 55.435 92 41 0 39 130 55 146 4.02e-26 104
MS.gene06643.t1 MTR_5g039000 54.255 94 43 0 37 130 76 169 5.11e-26 103
MS.gene06643.t1 MTR_3g086790 51.064 94 46 0 37 130 59 152 1.36e-25 102
MS.gene06643.t1 MTR_7g103340 40.690 145 58 4 37 160 62 199 4.88e-25 99.8
MS.gene06643.t1 MTR_8g469430 54.348 92 42 0 39 130 90 181 9.30e-25 100
MS.gene06643.t1 MTR_3g080100 49.474 95 48 0 36 130 18 112 1.03e-24 98.2
MS.gene06643.t1 MTR_3g080100 49.474 95 48 0 36 130 57 151 1.61e-24 99.0
MS.gene06643.t1 MTR_7g010020 47.826 92 48 0 39 130 81 172 8.24e-21 89.4
MS.gene06643.t1 MTR_5g019680 47.368 114 46 2 31 130 39 152 1.06e-20 87.4
MS.gene06643.t1 MTR_5g019650 52.577 97 38 2 31 120 39 134 2.12e-19 84.3
MS.gene06643.t1 MTR_4g100550 45.652 92 43 1 39 130 121 205 1.48e-13 69.3
MS.gene06643.t1 MTR_7g093430 42.857 98 49 1 33 130 155 245 1.47e-12 66.6
MS.gene06643.t1 MTR_4g126900 42.857 84 48 0 41 124 91 174 2.24e-12 65.1
MS.gene06643.t1 MTR_4g097600 43.158 95 47 1 39 133 128 215 4.01e-12 65.1
MS.gene06643.t1 MTR_5g014890 43.750 96 53 1 39 133 124 219 7.44e-12 64.3
MS.gene06643.t1 MTR_1g017090 43.158 95 47 1 39 133 131 218 1.25e-11 63.9
MS.gene06643.t1 MTR_3g103590 45.333 75 41 0 36 110 143 217 1.91e-11 63.5
MS.gene06643.t1 MTR_1g054285 39.423 104 56 1 33 136 149 245 2.66e-11 62.8
MS.gene06643.t1 MTR_7g027265 31.250 144 73 4 35 177 17 135 2.68e-11 60.5
MS.gene06643.t1 MTR_8g006705 43.478 92 45 1 39 130 147 231 2.95e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06643.t1 AT2G46680 69.307 101 31 0 30 130 24 124 9.76e-43 146
MS.gene06643.t1 AT2G46680 70.103 97 29 0 30 126 24 120 7.47e-41 141
MS.gene06643.t1 AT3G61890 68.132 91 29 0 41 131 33 123 2.33e-38 134
MS.gene06643.t1 AT3G01470 43.382 136 77 0 37 172 67 202 1.88e-29 112
MS.gene06643.t1 AT2G22430 56.383 94 41 0 37 130 61 154 7.00e-29 111
MS.gene06643.t1 AT4G40060 50.485 103 51 0 37 139 58 160 5.08e-28 108
MS.gene06643.t1 AT1G69780 54.255 94 43 0 37 130 84 177 1.79e-27 107
MS.gene06643.t1 AT5G15150 53.191 94 44 0 37 130 114 207 6.76e-26 103
MS.gene06643.t1 AT2G18550 50.943 106 45 1 39 137 62 167 1.21e-25 100
MS.gene06643.t1 AT5G65310 51.064 94 46 0 37 130 53 146 5.38e-25 100
MS.gene06643.t1 AT5G65310 51.064 94 46 0 37 130 71 164 5.91e-25 101
MS.gene06643.t1 AT4G36740 50.962 104 44 1 39 135 57 160 1.49e-24 98.2
MS.gene06643.t1 AT4G36740 50.962 104 44 1 39 135 56 159 1.86e-24 97.8
MS.gene06643.t1 AT5G66700 51.000 100 42 1 39 131 72 171 2.32e-24 97.8
MS.gene06643.t1 AT3G01220 48.936 94 48 0 37 130 86 179 1.80e-23 96.7
MS.gene06643.t1 AT5G03790 45.045 111 50 2 20 130 63 162 2.89e-23 95.1
MS.gene06643.t1 AT1G26960 42.969 128 65 2 11 130 36 163 4.47e-22 92.4
MS.gene06643.t1 AT5G03790 46.000 100 50 1 20 119 63 158 1.03e-21 89.7
MS.gene06643.t1 AT1G27045 40.299 134 74 2 9 138 35 166 4.90e-21 89.4
MS.gene06643.t1 AT1G27045 45.361 97 53 0 34 130 21 117 5.71e-20 85.1
MS.gene06643.t1 AT1G27045 45.361 97 53 0 34 130 21 117 5.71e-20 85.1
MS.gene06643.t1 AT1G27045 39.683 126 72 1 9 130 35 160 6.16e-20 85.9
MS.gene06643.t1 AT1G27045 45.361 97 53 0 34 130 13 109 6.64e-20 84.7
MS.gene06643.t1 AT2G36610 42.308 104 45 2 39 134 72 168 3.30e-19 83.2
MS.gene06643.t1 AT5G53980 39.604 101 57 1 29 129 6 102 2.76e-15 71.6
MS.gene06643.t1 AT2G44910 42.000 100 51 1 39 138 164 256 2.19e-12 66.2
MS.gene06643.t1 AT3G60390 42.000 100 51 1 39 138 163 255 6.08e-12 65.1
MS.gene06643.t1 AT2G22800 42.105 95 48 1 39 133 114 201 1.40e-11 63.5
MS.gene06643.t1 AT5G06710 41.837 98 50 1 33 130 185 275 2.06e-11 63.5
MS.gene06643.t1 AT4G16780 42.105 95 48 1 39 133 130 217 8.95e-11 61.2
MS.gene06643.t1 AT4G37790 41.053 95 49 1 39 133 127 214 9.09e-11 61.2

Find 35 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTCATGAAGGGTTTAA+AGG 0.209558 3.3:-76937648 MS.gene06643:CDS
AGCCTAGACAAGTTGCTATA+TGG 0.329458 3.3:-76937443 MS.gene06643:CDS
GCAAGTTGCATCTTCTTCCT+TGG 0.339735 3.3:+76937483 None:intergenic
TGAGGGTTTGGAGGATAAAA+TGG 0.360194 3.3:-76936896 MS.gene06643:CDS
AGCACAGAAGATGGTGGTTC+AGG 0.365057 3.3:-76936970 MS.gene06643:CDS
GGTTCAGAAGCTAAGTTATA+TGG 0.367405 3.3:-76937034 MS.gene06643:CDS
GAAGATGGTGGTTCAGGAAA+TGG 0.372815 3.3:-76936964 MS.gene06643:CDS
GAAATGGATACAACAATTGC+AGG 0.379643 3.3:-76936948 MS.gene06643:CDS
ATGCAACTTGCAAGAGATCT+TGG 0.385707 3.3:-76937471 MS.gene06643:CDS
TTCTAAGTTTCAGTGCCTTA+AGG 0.396105 3.3:-76937337 MS.gene06643:CDS
AAGACTCTTTCTCTTCCTTA+AGG 0.407960 3.3:+76937322 None:intergenic
TGATGAACAGATAAGATCAT+TGG 0.455417 3.3:-76937538 MS.gene06643:CDS
GTTCAAAACATGCAAGTTCA+AGG 0.470409 3.3:-76936612 MS.gene06643:CDS
GAAGAAGAGAATATTCATGA+AGG 0.503417 3.3:-76937657 MS.gene06643:CDS
CAGAATCAAAGTTGGAGCCA+AGG 0.518559 3.3:-76937500 MS.gene06643:CDS
ATTTGAGTCAGAATCAAAGT+TGG 0.518777 3.3:-76937508 MS.gene06643:CDS
AAGAAGAGAATATTCATGAA+GGG 0.519573 3.3:-76937656 MS.gene06643:CDS
AAGGAAAATAGCACAGAAGA+TGG 0.540540 3.3:-76936979 MS.gene06643:CDS
GAAAGAGTCTTTGCAATCAG+AGG 0.541327 3.3:-76937310 MS.gene06643:intron
AGAAGATAGAAAACAAGAGA+AGG 0.569555 3.3:-76937566 MS.gene06643:CDS
AAACATCGCATGATGGAGGA+AGG 0.574577 3.3:-76937008 MS.gene06643:CDS
AACCATATAGCAACTTGTCT+AGG 0.580258 3.3:+76937441 None:intergenic
TTCAGAACAGAAGAGCAAGA+TGG 0.602218 3.3:-76937419 MS.gene06643:CDS
TGATGGAGGAAGGGAAGCTA+AGG 0.606332 3.3:-76936998 MS.gene06643:CDS
TCAGAAGCTAAGTTATATGG+TGG 0.615960 3.3:-76937031 MS.gene06643:CDS
ATGGTGGAAACATCGCATGA+TGG 0.617224 3.3:-76937015 MS.gene06643:CDS
AACATCGCATGATGGAGGAA+GGG 0.628539 3.3:-76937007 MS.gene06643:CDS
GTGGAAACATCGCATGATGG+AGG 0.645127 3.3:-76937012 MS.gene06643:CDS
GGCCATATATAATATGATGG+AGG 0.645919 3.3:-76937679 None:intergenic
GAGCAAGATGGAAATCAAAG+AGG 0.650346 3.3:-76937407 MS.gene06643:CDS
GAAAATAGCACAGAAGATGG+TGG 0.663671 3.3:-76936976 MS.gene06643:CDS
AAAATGAAAGAAGCACAACA+TGG 0.668449 3.3:-76936855 MS.gene06643:CDS
ACAGAGAAAATTGAGGACAG+AGG 0.671559 3.3:-76936832 MS.gene06643:CDS
AACATGGACAGAGAAAATTG+AGG 0.710156 3.3:-76936839 MS.gene06643:CDS
TGGTACAATGTTGATCCATG+TGG 0.723453 3.3:-76936748 MS.gene06643:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGGAAAAAAAAATTCACA+GGG + chr3.3:76937159-76937178 None:intergenic 20.0%
!!! TCAAAAAAATGAAGTTTTGT+AGG - chr3.3:76937639-76937658 MS.gene06643:CDS 20.0%
! AAGAAGAGAATATTCATGAA+GGG - chr3.3:76936559-76936578 MS.gene06643:CDS 25.0%
! AATATTCATGAAGGGTTTAA+AGG - chr3.3:76936567-76936586 MS.gene06643:CDS 25.0%
! GTTAGGAAAAAAAAATTCAC+AGG + chr3.3:76937160-76937179 None:intergenic 25.0%
!! AAAGCAAAGATTTTCAAATG+AGG - chr3.3:76937301-76937320 MS.gene06643:intron 25.0%
!! AAGCAAAGATTTTCAAATGA+GGG - chr3.3:76937302-76937321 MS.gene06643:intron 25.0%
!! TTTTTCTTCTTTGAATTCTG+AGG + chr3.3:76936627-76936646 None:intergenic 25.0%
!!! AAACTTCATTTTTTTGAGCT+TGG + chr3.3:76937636-76937655 None:intergenic 25.0%
!!! GTGAATTTTTTTTTCCTAAC+AGG - chr3.3:76937160-76937179 MS.gene06643:intron 25.0%
AAGTTAATGTCGAGTCTATA+AGG + chr3.3:76937134-76937153 None:intergenic 30.0%
AATCATGCAATAGTTCTCAA+TGG - chr3.3:76937501-76937520 MS.gene06643:CDS 30.0%
AGAAGATAGAAAACAAGAGA+AGG - chr3.3:76936649-76936668 MS.gene06643:intron 30.0%
ATTTGAGTCAGAATCAAAGT+TGG - chr3.3:76936707-76936726 MS.gene06643:intron 30.0%
CTTGAAAAAGGAACAAAGAA+TGG - chr3.3:76937581-76937600 MS.gene06643:CDS 30.0%
GAAGAAGAGAATATTCATGA+AGG - chr3.3:76936558-76936577 MS.gene06643:CDS 30.0%
TGACAACAACACTTGAAAAA+TGG - chr3.3:76937447-76937466 MS.gene06643:CDS 30.0%
TGATGAACAGATAAGATCAT+TGG - chr3.3:76936677-76936696 MS.gene06643:intron 30.0%
! AAAATGAAAGAAGCACAACA+TGG - chr3.3:76937360-76937379 MS.gene06643:CDS 30.0%
! AAGATTTTCAAATGAGGGTT+TGG - chr3.3:76937307-76937326 MS.gene06643:intron 30.0%
!! CATTCTTTGTTCCTTTTTCA+AGG + chr3.3:76937583-76937602 None:intergenic 30.0%
!! TTTTGTAGGCTTGATTTGAT+AGG - chr3.3:76937653-76937672 MS.gene06643:CDS 30.0%
!!! TTTTCAAGTGTTGTTGTCAA+GGG + chr3.3:76937446-76937465 None:intergenic 30.0%
!!! TTTTTCAAGTGTTGTTGTCA+AGG + chr3.3:76937447-76937466 None:intergenic 30.0%
AAACATTGCAACATAGAGCA+TGG + chr3.3:76937553-76937572 None:intergenic 35.0%
AACATGGACAGAGAAAATTG+AGG - chr3.3:76937376-76937395 MS.gene06643:CDS 35.0%
AACCATATAGCAACTTGTCT+AGG + chr3.3:76936777-76936796 None:intergenic 35.0%
AAGACTCTTTCTCTTCCTTA+AGG + chr3.3:76936896-76936915 None:intergenic 35.0%
AAGGAAAATAGCACAGAAGA+TGG - chr3.3:76937236-76937255 MS.gene06643:intron 35.0%
ACATGCTCAAGCTTAGTATT+AGG - chr3.3:76937079-76937098 MS.gene06643:intron 35.0%
ATGCAATAGTTCTCAATGGT+TGG - chr3.3:76937505-76937524 MS.gene06643:CDS 35.0%
ATGCTCTATGTTGCAATGTT+TGG - chr3.3:76937552-76937571 MS.gene06643:CDS 35.0%
GAAATGGATACAACAATTGC+AGG - chr3.3:76937267-76937286 MS.gene06643:intron 35.0%
GATTGATCTAACATACCACA+TGG + chr3.3:76937485-76937504 None:intergenic 35.0%
GGTTCAGAAGCTAAGTTATA+TGG - chr3.3:76937181-76937200 MS.gene06643:intron 35.0%
GTTCAAAACATGCAAGTTCA+AGG - chr3.3:76937603-76937622 MS.gene06643:CDS 35.0%
TCAGAAGCTAAGTTATATGG+TGG - chr3.3:76937184-76937203 MS.gene06643:intron 35.0%
TCAGAGGTAAGTTAGTAGTT+TGG - chr3.3:76936921-76936940 MS.gene06643:CDS 35.0%
TTCTAAGTTTCAGTGCCTTA+AGG - chr3.3:76936878-76936897 MS.gene06643:CDS 35.0%
! ATTTTCAAATGAGGGTTTGG+AGG - chr3.3:76937310-76937329 MS.gene06643:intron 35.0%
! GTTCTCAATGGTTGGATTTT+TGG - chr3.3:76937513-76937532 MS.gene06643:CDS 35.0%
!! GTTTGGTGTTTCCTTGAAAA+AGG - chr3.3:76937569-76937588 MS.gene06643:CDS 35.0%
ACAGAGAAAATTGAGGACAG+AGG - chr3.3:76937383-76937402 MS.gene06643:CDS 40.0%
ACTTAGCTTCTGAACCTGTT+AGG + chr3.3:76937177-76937196 None:intergenic 40.0%
AGCCTAGACAAGTTGCTATA+TGG - chr3.3:76936772-76936791 MS.gene06643:CDS 40.0%
ATGCAACTTGCAAGAGATCT+TGG - chr3.3:76936744-76936763 MS.gene06643:intron 40.0%
GAAAATAGCACAGAAGATGG+TGG - chr3.3:76937239-76937258 MS.gene06643:intron 40.0%
GAAAGAGTCTTTGCAATCAG+AGG - chr3.3:76936905-76936924 MS.gene06643:CDS 40.0%
GACTCAGCTCGTTAAATACT+CGG - chr3.3:76937031-76937050 MS.gene06643:CDS 40.0%
GAGCAAGATGGAAATCAAAG+AGG - chr3.3:76936808-76936827 MS.gene06643:CDS 40.0%
TGAGGGTTTGGAGGATAAAA+TGG - chr3.3:76937319-76937338 MS.gene06643:CDS 40.0%
TGGTACAATGTTGATCCATG+TGG - chr3.3:76937467-76937486 MS.gene06643:CDS 40.0%
TTCAGAACAGAAGAGCAAGA+TGG - chr3.3:76936796-76936815 MS.gene06643:CDS 40.0%
CAGAATCAAAGTTGGAGCCA+AGG - chr3.3:76936715-76936734 MS.gene06643:intron 45.0%
GCAAGTTGCATCTTCTTCCT+TGG + chr3.3:76936735-76936754 None:intergenic 45.0%
! AAACATCGCATGATGGAGGA+AGG - chr3.3:76937207-76937226 MS.gene06643:intron 45.0%
! AACATCGCATGATGGAGGAA+GGG - chr3.3:76937208-76937227 MS.gene06643:intron 45.0%
! ATGGTGGAAACATCGCATGA+TGG - chr3.3:76937200-76937219 MS.gene06643:intron 45.0%
! GAAGATGGTGGTTCAGGAAA+TGG - chr3.3:76937251-76937270 MS.gene06643:intron 45.0%
GTGGAAACATCGCATGATGG+AGG - chr3.3:76937203-76937222 MS.gene06643:intron 50.0%
TGATGGAGGAAGGGAAGCTA+AGG - chr3.3:76937217-76937236 MS.gene06643:intron 50.0%
! AGCACAGAAGATGGTGGTTC+AGG - chr3.3:76937245-76937264 MS.gene06643:intron 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 76936549 76937688 76936549 ID=MS.gene06643
chr3.3 mRNA 76936549 76937688 76936549 ID=MS.gene06643.t1;Parent=MS.gene06643
chr3.3 exon 76937311 76937688 76937311 ID=MS.gene06643.t1.exon1;Parent=MS.gene06643.t1
chr3.3 CDS 76937311 76937688 76937311 ID=cds.MS.gene06643.t1;Parent=MS.gene06643.t1
chr3.3 exon 76936749 76937055 76936749 ID=MS.gene06643.t1.exon2;Parent=MS.gene06643.t1
chr3.3 CDS 76936749 76937055 76936749 ID=cds.MS.gene06643.t1;Parent=MS.gene06643.t1
chr3.3 exon 76936549 76936646 76936549 ID=MS.gene06643.t1.exon3;Parent=MS.gene06643.t1
chr3.3 CDS 76936549 76936646 76936549 ID=cds.MS.gene06643.t1;Parent=MS.gene06643.t1
Gene Sequence

>MS.gene06643

ATGATGGAGGAAGAAGAGAATATTCATGAAGGGTTTAAAGGAAAAAATGTTGAAAGCATGAGTTGCTTAGAGACACCTCAGAATTCAAAGAAGAAAAACAAGAAGATAGAAAACAAGAGAAGGTTTAGTGATGAACAGATAAGATCATTGGAATGCATATTTGAGTCAGAATCAAAGTTGGAGCCAAGGAAGAAGATGCAACTTGCAAGAGATCTTGGTTTGCAGCCTAGACAAGTTGCTATATGGTTTCAGAACAGAAGAGCAAGATGGAAATCAAAGAGGATAGAACAAGAATACAGAAAACTCAAAGATGAATATGATAATTTAGCTTCTAAGTTTCAGTGCCTTAAGGAAGAGAAAGAGTCTTTGCAATCAGAGGTAAGTTAGTAGTTTGGTTCTTGTAATTGTAACATAACTAGAGTTGTGTCTCGATAAACAGCTTAATTTAATTTAAACGAGTTGAGCTATTCGTGAACTATTCAGACTCAGCTCGTTAAATACTCGGTTATGTTCGTTTGTTTAGTTAAATAACATGCTCAAGCTTAGTATTAGGCTCGATTAATTAAATGAGTTGAACTTAAACCTTATAGACTCGACATTAACTTAACCCTGTGAATTTTTTTTTCCTAACAGGTTCAGAAGCTAAGTTATATGGTGGAAACATCGCATGATGGAGGAAGGGAAGCTAAGGAAAATAGCACAGAAGATGGTGGTTCAGGAAATGGATACAACAATTGCAGGTTTGAAGAAACAAAGCAAAGATTTTCAAATGAGGGTTTGGAGGATAAAATGGTCATTTATTCAGATGATCAAAATGAAAGAAGCACAACATGGACAGAGAAAATTGAGGACAGAGGAAATCAACTTTTAAGAAATGATGATCATGCAGAAATTCCCTTGACAACAACACTTGAAAAATGGTACAATGTTGATCCATGTGGTATGTTAGATCAATCATGCAATAGTTCTCAATGGTTGGATTTTTGGACTTGAAAATTGCACCATGCTCTATGTTGCAATGTTTGGTGTTTCCTTGAAAAAGGAACAAAGAATGGTTCAAAACATGCAAGTTCAAGGAGAATGTCCAAGCTCAAAAAAATGAAGTTTTGTAGGCTTGATTTGATAGGTAGAGAATTATGA

Protein sequence

>MS.gene06643.t1

MMEEEENIHEGFKGKNVESMSCLETPQNSKKKNKKIENKRRFSDEQIRSLECIFESESKLEPRKKMQLARDLGLQPRQVAIWFQNRRARWKSKRIEQEYRKLKDEYDNLASKFQCLKEEKESLQSEVQKLSYMVETSHDGGREAKENSTEDGGSGNGYNNCRFEETKQRFSNEGLEDKMVIYSDDQNERSTTWTEKIEDRGNQLLRNDDHAEIPLTTTLEKWYNVDPCGTKNGSKHASSRRMSKLKKMKFCRLDLIGREL