AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56441


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56441.t1 MTR_4g083590 96.126 413 15 1 1 412 13 425 0.0 820
MS.gene56441.t1 MTR_2g028900 70.353 425 108 5 3 410 9 432 0.0 604
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56441.t1 AT3G05320 62.529 427 138 9 8 412 16 442 0.0 534
MS.gene56441.t1 AT3G05320 62.529 427 138 9 8 412 16 442 0.0 534
MS.gene56441.t1 AT3G05320 62.529 427 138 9 8 412 16 442 0.0 534

Find 63 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCAAAATTCTTCAATAT+TGG 0.230483 4.3:-27933524 MS.gene56441:CDS
CGCAAGAATGTTTCATCATA+TGG 0.247238 4.3:-27934285 MS.gene56441:CDS
CTTTCTTGTGGCATGATCAT+TGG 0.261574 4.3:-27933864 MS.gene56441:CDS
AACACAAAAGATGATCAAAT+TGG 0.268660 4.3:-27933326 MS.gene56441:CDS
GTGGACAGTTGAAAGGGATT+TGG 0.319886 4.3:-27933956 MS.gene56441:CDS
ACCTTTGCATAGTCCTTAAC+AGG 0.326962 4.3:+27933841 None:intergenic
CCCTTGCAAATGCAATCTTT+TGG 0.340133 4.3:+27934202 None:intergenic
ATTGCAGACTGAATTAAATA+AGG 0.349906 4.3:+27933430 None:intergenic
CCAAGTTTCTTCTTCTCATA+AGG 0.367277 4.3:+27933478 None:intergenic
GTTGGTAACCATGATGATAA+AGG 0.386254 4.3:-27933748 MS.gene56441:CDS
ATGAATTACCTTTATCATCA+TGG 0.396445 4.3:+27933740 None:intergenic
ATTCTTCAATATTGGAAGGT+TGG 0.400853 4.3:-27933516 MS.gene56441:CDS
ACATGAGAGTAGAAATTGAT+TGG 0.407692 4.3:-27933687 MS.gene56441:CDS
TAGTAGCAAGAATGAGATAA+TGG 0.408374 4.3:-27933614 MS.gene56441:CDS
ACTGCAAGATAAAGAACAAC+TGG 0.413545 4.3:+27933559 None:intergenic
TTTAATCCCCACACAATTTG+AGG 0.419362 4.3:+27934228 None:intergenic
TTGAGAGCTGATATCTTTGT+TGG 0.421531 4.3:-27933391 MS.gene56441:CDS
AACACCTTGTCAAATGAAAT+CGG 0.421780 4.3:+27934090 None:intergenic
CATTCTTGCGCGTAAATACA+AGG 0.427389 4.3:+27934298 None:intergenic
ATTGAGGTGATTAAGATTGA+TGG 0.430449 4.3:-27933895 MS.gene56441:CDS
TTTATATTATATGCAAAAGA+AGG 0.438653 4.3:+27933268 None:intergenic
GCCTCTATGATTAGAAAAGT+TGG 0.443332 4.3:-27933766 MS.gene56441:CDS
GCATGATCATTGGCCTGTTA+AGG 0.447996 4.3:-27933854 MS.gene56441:CDS
ATATTGGAAGGTTGGAAAGA+AGG 0.448216 4.3:-27933508 MS.gene56441:CDS
AATGATCATGCCACAAGAAA+GGG 0.451098 4.3:+27933866 None:intergenic
ATGAGATAATGGAAAGAGTA+GGG 0.459739 4.3:-27933603 MS.gene56441:CDS
ACACAAAAGATGATCAAATT+GGG 0.466064 4.3:-27933325 MS.gene56441:CDS
CAATGATCATGCCACAAGAA+AGG 0.479742 4.3:+27933865 None:intergenic
GGAGGTTCCTCAAATTGTGT+GGG 0.483333 4.3:-27934235 MS.gene56441:CDS
CCTTATGAGAAGAAGAAACT+TGG 0.492913 4.3:-27933478 MS.gene56441:CDS
AATGAGATAATGGAAAGAGT+AGG 0.495512 4.3:-27933604 MS.gene56441:CDS
TGGAGGTTCCTCAAATTGTG+TGG 0.500076 4.3:-27934236 MS.gene56441:CDS
ACCAAAAGATTGCATTTGCA+AGG 0.505247 4.3:-27934203 MS.gene56441:CDS
TTGAGGTGATTAAGATTGAT+GGG 0.506436 4.3:-27933894 MS.gene56441:CDS
CAAAATTCTTCAATATTGGA+AGG 0.510361 4.3:-27933520 MS.gene56441:CDS
AATTGAATGGTCCTATGAGA+TGG 0.511997 4.3:-27933240 MS.gene56441:CDS
AATATAAAAGGAGAATTGAA+TGG 0.513612 4.3:-27933253 MS.gene56441:CDS
ATATTTGTAACCCATCTCAT+AGG 0.515941 4.3:+27933229 None:intergenic
CTCATGTGAACAGCAACATA+AGG 0.518574 4.3:+27933703 None:intergenic
CCAAAAGATTGCATTTGCAA+GGG 0.530122 4.3:-27934202 MS.gene56441:CDS
CTGATAAAGTTAATGGGTGG+TGG 0.531434 4.3:-27934402 None:intergenic
AGCCTTCATGCTATCAGCTA+TGG 0.542544 4.3:-27933196 MS.gene56441:CDS
GTAGAAAGTGGACAGTTGAA+AGG 0.547343 4.3:-27933963 MS.gene56441:CDS
ATGGGAAAACCCCTTTCTTG+TGG 0.557806 4.3:-27933876 MS.gene56441:CDS
GCCTGTTAAGGACTATGCAA+AGG 0.559420 4.3:-27933842 MS.gene56441:CDS
TAGAAAGTGGACAGTTGAAA+GGG 0.571713 4.3:-27933962 MS.gene56441:CDS
GAGGTTCCTCAAATTGTGTG+GGG 0.573751 4.3:-27934234 MS.gene56441:CDS
ATTGAATGGTCCTATGAGAT+GGG 0.575314 4.3:-27933239 MS.gene56441:CDS
GTTGAATTGCAGAAAGGAAG+CGG 0.576987 4.3:-27933985 MS.gene56441:CDS
TCTCTTGAACAGAACAGAAG+TGG 0.597718 4.3:-27934007 MS.gene56441:CDS
ATAGCTTTCAACAAGAAATG+TGG 0.601889 4.3:+27933167 None:intergenic
ATTGATTTCCTTGTAAAACA+AGG 0.609636 4.3:+27934122 None:intergenic
CTTGAGTATATTCATCAACC+AGG 0.612021 4.3:+27933803 None:intergenic
GAAGTGGTTGAATTGCAGAA+AGG 0.612730 4.3:-27933991 MS.gene56441:CDS
AAATTTAATGAACTGTGTAG+TGG 0.613501 4.3:-27934057 MS.gene56441:CDS
AGAAAGGAAGCGGTAGAAAG+TGG 0.619057 4.3:-27933975 MS.gene56441:CDS
TGCCATAGCTGATAGCATGA+AGG 0.627885 4.3:+27933194 None:intergenic
TGTGTGGAAGTGATGTAAGA+TGG 0.635629 4.3:-27933291 MS.gene56441:CDS
AACAAGGAAGCACTAAGACT+AGG 0.654550 4.3:+27934138 None:intergenic
ATGATCATGCCACAAGAAAG+GGG 0.657561 4.3:+27933867 None:intergenic
ACAACCGATTTCATTTGACA+AGG 0.662499 4.3:-27934094 MS.gene56441:CDS
TTGGGTGTGAATGAAATGTG+TGG 0.683523 4.3:-27933307 MS.gene56441:CDS
AAACTGATAAAGTTAATGGG+TGG 0.765999 4.3:-27934405 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATTATATGCAAAAGA+AGG + chr4.3:27934299-27934318 None:intergenic 15.0%
!!! TCTTTTGCATATAATATAAA+AGG - chr4.3:27934299-27934318 MS.gene56441:CDS 15.0%
!! AATATAAAAGGAGAATTGAA+TGG - chr4.3:27934311-27934330 MS.gene56441:CDS 20.0%
!! ACTTCAAAATTCTTCAATAT+TGG - chr4.3:27934040-27934059 MS.gene56441:CDS 20.0%
! AAATTTAATGAACTGTGTAG+TGG - chr4.3:27933507-27933526 MS.gene56441:CDS 25.0%
! AACACAAAAGATGATCAAAT+TGG - chr4.3:27934238-27934257 MS.gene56441:CDS 25.0%
! ACACAAAAGATGATCAAATT+GGG - chr4.3:27934239-27934258 MS.gene56441:CDS 25.0%
! ATGAATTACCTTTATCATCA+TGG + chr4.3:27933827-27933846 None:intergenic 25.0%
! ATTGCAGACTGAATTAAATA+AGG + chr4.3:27934137-27934156 None:intergenic 25.0%
! CAAAATTCTTCAATATTGGA+AGG - chr4.3:27934044-27934063 MS.gene56441:CDS 25.0%
! GATGATTCATTGCAAAAAAT+TGG - chr4.3:27933899-27933918 MS.gene56441:CDS 25.0%
!! ATTGATTTCCTTGTAAAACA+AGG + chr4.3:27933445-27933464 None:intergenic 25.0%
!! CAGTATAAAAATGGTTCTTT+TGG - chr4.3:27933642-27933661 MS.gene56441:CDS 25.0%
!! TGTTCAACAACAAAAGTTTT+TGG - chr4.3:27933308-27933327 MS.gene56441:CDS 25.0%
!!! TATTGCTGTTTTGATTCTAA+GGG - chr4.3:27933224-27933243 MS.gene56441:CDS 25.0%
!!! TTATTGCTGTTTTGATTCTA+AGG - chr4.3:27933223-27933242 MS.gene56441:CDS 25.0%
AACACCTTGTCAAATGAAAT+CGG + chr4.3:27933477-27933496 None:intergenic 30.0%
ACATGAGAGTAGAAATTGAT+TGG - chr4.3:27933877-27933896 MS.gene56441:CDS 30.0%
ATATTTGTAACCCATCTCAT+AGG + chr4.3:27934338-27934357 None:intergenic 30.0%
ATTCTTCAATATTGGAAGGT+TGG - chr4.3:27934048-27934067 MS.gene56441:CDS 30.0%
ATTGAGGTGATTAAGATTGA+TGG - chr4.3:27933669-27933688 MS.gene56441:CDS 30.0%
CAGTTGAGACAGTATAAAAA+TGG - chr4.3:27933633-27933652 MS.gene56441:CDS 30.0%
TAGTAGCAAGAATGAGATAA+TGG - chr4.3:27933950-27933969 MS.gene56441:CDS 30.0%
TTGAGGTGATTAAGATTGAT+GGG - chr4.3:27933670-27933689 MS.gene56441:CDS 30.0%
! ACCAACTTTTCTAATCATAG+AGG + chr4.3:27933802-27933821 None:intergenic 30.0%
!! AATGAGATAATGGAAAGAGT+AGG - chr4.3:27933960-27933979 MS.gene56441:CDS 30.0%
!! ATGAGATAATGGAAAGAGTA+GGG - chr4.3:27933961-27933980 MS.gene56441:CDS 30.0%
!! TCAACAACAAAAGTTTTTGG+AGG - chr4.3:27933311-27933330 MS.gene56441:CDS 30.0%
!!! TGTAGTGGTTTTGTTCAATT+GGG - chr4.3:27933522-27933541 MS.gene56441:CDS 30.0%
AATGATCATGCCACAAGAAA+GGG + chr4.3:27933701-27933720 None:intergenic 35.0%
AATTGAATGGTCCTATGAGA+TGG - chr4.3:27934324-27934343 MS.gene56441:CDS 35.0%
ACCAAAAGATTGCATTTGCA+AGG - chr4.3:27933361-27933380 MS.gene56441:CDS 35.0%
ACTGCAAGATAAAGAACAAC+TGG + chr4.3:27934008-27934027 None:intergenic 35.0%
ATATTGGAAGGTTGGAAAGA+AGG - chr4.3:27934056-27934075 MS.gene56441:CDS 35.0%
ATTGAATGGTCCTATGAGAT+GGG - chr4.3:27934325-27934344 MS.gene56441:CDS 35.0%
CCAAAAGATTGCATTTGCAA+GGG - chr4.3:27933362-27933381 MS.gene56441:CDS 35.0%
CCAAGTTTCTTCTTCTCATA+AGG + chr4.3:27934089-27934108 None:intergenic 35.0%
CCTTATGAGAAGAAGAAACT+TGG - chr4.3:27934086-27934105 MS.gene56441:CDS 35.0%
CGCAAGAATGTTTCATCATA+TGG - chr4.3:27933279-27933298 MS.gene56441:CDS 35.0%
CTTGAGTATATTCATCAACC+AGG + chr4.3:27933764-27933783 None:intergenic 35.0%
GCCTCTATGATTAGAAAAGT+TGG - chr4.3:27933798-27933817 MS.gene56441:CDS 35.0%
TAGAAAGTGGACAGTTGAAA+GGG - chr4.3:27933602-27933621 MS.gene56441:CDS 35.0%
TTGAGAGCTGATATCTTTGT+TGG - chr4.3:27934173-27934192 MS.gene56441:CDS 35.0%
TTTAATCCCCACACAATTTG+AGG + chr4.3:27933339-27933358 None:intergenic 35.0%
! ACAACCGATTTCATTTGACA+AGG - chr4.3:27933470-27933489 MS.gene56441:CDS 35.0%
!! GTTGGTAACCATGATGATAA+AGG - chr4.3:27933816-27933835 MS.gene56441:CDS 35.0%
!! TGGTTCTTTTGGTGATATTG+AGG - chr4.3:27933653-27933672 MS.gene56441:CDS 35.0%
!!! GTGTAGTGGTTTTGTTCAAT+TGG - chr4.3:27933521-27933540 MS.gene56441:CDS 35.0%
!!! TAGTGCTTCCTTGTTTTACA+AGG - chr4.3:27933434-27933453 MS.gene56441:CDS 35.0%
ACCTTTGCATAGTCCTTAAC+AGG + chr4.3:27933726-27933745 None:intergenic 40.0%
ATGATCATGCCACAAGAAAG+GGG + chr4.3:27933700-27933719 None:intergenic 40.0%
CAATGATCATGCCACAAGAA+AGG + chr4.3:27933702-27933721 None:intergenic 40.0%
CATTCTTGCGCGTAAATACA+AGG + chr4.3:27933269-27933288 None:intergenic 40.0%
CTCATGTGAACAGCAACATA+AGG + chr4.3:27933864-27933883 None:intergenic 40.0%
CTTTCTTGTGGCATGATCAT+TGG - chr4.3:27933700-27933719 MS.gene56441:CDS 40.0%
GAAGTGGTTGAATTGCAGAA+AGG - chr4.3:27933573-27933592 MS.gene56441:CDS 40.0%
GTAGAAAGTGGACAGTTGAA+AGG - chr4.3:27933601-27933620 MS.gene56441:CDS 40.0%
GTTGAATTGCAGAAAGGAAG+CGG - chr4.3:27933579-27933598 MS.gene56441:CDS 40.0%
TCTCTTGAACAGAACAGAAG+TGG - chr4.3:27933557-27933576 MS.gene56441:CDS 40.0%
TGTGTGGAAGTGATGTAAGA+TGG - chr4.3:27934273-27934292 MS.gene56441:CDS 40.0%
TTGGGTGTGAATGAAATGTG+TGG - chr4.3:27934257-27934276 MS.gene56441:CDS 40.0%
! CCCTTGCAAATGCAATCTTT+TGG + chr4.3:27933365-27933384 None:intergenic 40.0%
! CGCGTAAATACAAGGTTTTG+AGG + chr4.3:27933261-27933280 None:intergenic 40.0%
!! AACAAGGAAGCACTAAGACT+AGG + chr4.3:27933429-27933448 None:intergenic 40.0%
AGAAAGGAAGCGGTAGAAAG+TGG - chr4.3:27933589-27933608 MS.gene56441:CDS 45.0%
AGCCTTCATGCTATCAGCTA+TGG - chr4.3:27934368-27934387 MS.gene56441:CDS 45.0%
ATGGGAAAACCCCTTTCTTG+TGG - chr4.3:27933688-27933707 MS.gene56441:CDS 45.0%
GAGGTTCCTCAAATTGTGTG+GGG - chr4.3:27933330-27933349 MS.gene56441:CDS 45.0%
GCCTGTTAAGGACTATGCAA+AGG - chr4.3:27933722-27933741 MS.gene56441:CDS 45.0%
GGAGGTTCCTCAAATTGTGT+GGG - chr4.3:27933329-27933348 MS.gene56441:CDS 45.0%
GTGGACAGTTGAAAGGGATT+TGG - chr4.3:27933608-27933627 MS.gene56441:CDS 45.0%
TGCCATAGCTGATAGCATGA+AGG + chr4.3:27934373-27934392 None:intergenic 45.0%
TGGAGGTTCCTCAAATTGTG+TGG - chr4.3:27933328-27933347 MS.gene56441:CDS 45.0%
! GCATGATCATTGGCCTGTTA+AGG - chr4.3:27933710-27933729 MS.gene56441:CDS 45.0%
!!! GGTTTTTGAGTGCTTAGTCC+TGG - chr4.3:27933743-27933762 MS.gene56441:CDS 45.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 27933174 27934412 27933174 ID=MS.gene56441
chr4.3 mRNA 27933174 27934412 27933174 ID=MS.gene56441.t1;Parent=MS.gene56441
chr4.3 exon 27933174 27934412 27933174 ID=MS.gene56441.t1.exon1;Parent=MS.gene56441.t1
chr4.3 CDS 27933174 27934412 27933174 ID=cds.MS.gene56441.t1;Parent=MS.gene56441.t1
Gene Sequence

>MS.gene56441

ATGGGTGGTGGCAATCTAAATTCATTAGTTTGCAAGATTGTGTTTTTGTTTATTGCTGTTTTGATTCTAAGGGCATTGTTGCTACCTCAAAACCTTGTATTTACGCGCAAGAATGTTTCATCATATGGTTTTGTTGTTCAACAACAAAAGTTTTTGGAGGTTCCTCAAATTGTGTGGGGATTAAACAACCAAAAGATTGCATTTGCAAGGGCTTGTCTTACTGCAAGAATGTTGAACAGAACACTATTGATGCCTAGTCTTAGTGCTTCCTTGTTTTACAAGGAAATCAATCTTTTACAACCGATTTCATTTGACAAGGTGTTTCAATTTGATAAATTTAATGAACTGTGTAGTGGTTTTGTTCAATTGGGTCGTTACTCTGATCTCTTGAACAGAACAGAAGTGGTTGAATTGCAGAAAGGAAGCGGTAGAAAGTGGACAGTTGAAAGGGATTTGGATCAGTTGAGACAGTATAAAAATGGTTCTTTTGGTGATATTGAGGTGATTAAGATTGATGGGAAAACCCCTTTCTTGTGGCATGATCATTGGCCTGTTAAGGACTATGCAAAGGTTTTTGAGTGCTTAGTCCTGGTTGATGAATATACTCAAGAAGCTGATAGAGTTGCCTCTATGATTAGAAAAGTTGGTAACCATGATGATAAAGGTAATTCATTTCAAAATTATGTGCCTTATGTTGCTGTTCACATGAGAGTAGAAATTGATTGGATGATTCATTGCAAAAAATTGGAGCAAAGATTGAACACTAATCAAATATGTAGTAGCAAGAATGAGATAATGGAAAGAGTAGGGAACATTGAAAGTCTTAAGACTCCAGTTGTTCTTTATCTTGCAGTAGCTGATAAACTACTTCAAAATTCTTCAATATTGGAAGGTTGGAAAGAAGGTTTTCTACCTTATGAGAAGAAGAAACTTGGTGTTGATAAAATATATGCAAAATATCCTTATTTAATTCAGTCTGCAATTGATTATGAAGTGTGTTTGAGAGCTGATATCTTTGTTGGTAACAGTTTCTCTACATTTTCAAGTCTTATAGTTCTTGAAAGAACACAAAAGATGATCAAATTGGGTGTGAATGAAATGTGTGGAAGTGATGTAAGATGGCCTTCTTTTGCATATAATATAAAAGGAGAATTGAATGGTCCTATGAGATGGGTTACAAATATGTCTGATTCAAGCCTTCATGCTATCAGCTATGGCACTAACCACATTTCTTGTTGA

Protein sequence

>MS.gene56441.t1

MGGGNLNSLVCKIVFLFIAVLILRALLLPQNLVFTRKNVSSYGFVVQQQKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEINLLQPISFDKVFQFDKFNELCSGFVQLGRYSDLLNRTEVVELQKGSGRKWTVERDLDQLRQYKNGSFGDIEVIKIDGKTPFLWHDHWPVKDYAKVFECLVLVDEYTQEADRVASMIRKVGNHDDKGNSFQNYVPYVAVHMRVEIDWMIHCKKLEQRLNTNQICSSKNEIMERVGNIESLKTPVVLYLAVADKLLQNSSILEGWKEGFLPYEKKKLGVDKIYAKYPYLIQSAIDYEVCLRADIFVGNSFSTFSSLIVLERTQKMIKLGVNEMCGSDVRWPSFAYNIKGELNGPMRWVTNMSDSSLHAISYGTNHISC