AlfalfaGEDB | MS.gene060096

M. sativa cultivar XinJiangDaYe / MS.gene060096

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060096.t1	XP_003619561.1	86.2	203	26	1	1	201	1	203	4.50E-97	364

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060096.t1	O82711	48.1	212	92	10	1	197	1	209	1.6e-39	164.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060096.t1	G7KMH6	86.2	203	26	1	1	201	1	203	3.2e-97	364.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051232	MS.gene060096	0.80333	3.67E-49	-1.69E-46
MS.gene051364	MS.gene060096	0.801702	7.95E-49	-1.69E-46
MS.gene058268	MS.gene060096	0.804792	1.82E-49	-1.69E-46
MS.gene060096	MS.gene060595	0.806096	9.71E-50	-1.69E-46
MS.gene060096	MS.gene064019	0.801927	7.15E-49	-1.69E-46
MS.gene060096	MS.gene065613	0.908984	9.40E-82	-1.69E-46
MS.gene060096	MS.gene069874	0.808704	2.72E-50	-1.69E-46
MS.gene060096	MS.gene24883	0.817945	2.53E-52	-1.69E-46
MS.gene060096	MS.gene30456	0.806526	7.88E-50	-1.69E-46
MS.gene060096	MS.gene36005	0.817865	2.64E-52	-1.69E-46
MS.gene060096	MS.gene36358	0.840611	7.83E-58	-1.69E-46
MS.gene060096	MS.gene37538	0.800069	1.71E-48	-1.69E-46
MS.gene060096	MS.gene46057	0.807139	5.85E-50	-1.69E-46
MS.gene060096	MS.gene47902	0.806065	9.86E-50	-1.69E-46
MS.gene060096	MS.gene51018	0.812985	3.22E-51	-1.69E-46
MS.gene060096	MS.gene52027	0.80483	1.79E-49	-1.69E-46
MS.gene060096	MS.gene55961	0.812782	3.57E-51	-1.69E-46
MS.gene060096	MS.gene56389	0.811261	7.65E-51	-1.69E-46
MS.gene060096	MS.gene59487	0.808577	2.89E-50	-1.69E-46
MS.gene060096	MS.gene67889	0.803734	3.03E-49	-1.69E-46
MS.gene060096	MS.gene75048	0.810712	1.01E-50	-1.69E-46
MS.gene060096	MS.gene86145	0.81462	1.40E-51	-1.69E-46
MS.gene060096	MS.gene88666	0.814752	1.31E-51	-1.69E-46
MS.gene060096	MS.gene91453	0.803035	4.22E-49	-1.69E-46
MS.gene060096	MS.gene93995	0.804978	1.67E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060096.t1	MTR_6g059530	86.207	203	26	1	1	201	1	203	2.46e-115	327
MS.gene060096.t1	MTR_6g059410	85.222	203	27	2	1	201	1	202	6.64e-112	318
MS.gene060096.t1	MTR_6g059650	57.216	194	73	5	17	201	19	211	2.56e-66	202
MS.gene060096.t1	MTR_6g065460	53.659	205	82	6	1	193	1	204	2.92e-55	174
MS.gene060096.t1	MTR_6g059760	48.113	212	87	12	1	196	1	205	7.21e-47	153
MS.gene060096.t1	MTR_6g065460	49.000	200	75	6	1	193	1	180	1.30e-45	149
MS.gene060096.t1	MTR_6g044780	43.662	213	105	9	1	199	1	212	2.19e-41	140
MS.gene060096.t1	MTR_6g044810	41.784	213	109	8	1	199	1	212	1.18e-39	135
MS.gene060096.t1	MTR_3g463590	43.750	192	95	7	20	201	23	211	4.94e-39	133
MS.gene060096.t1	MTR_6g059680	46.500	200	88	8	18	201	20	216	8.86e-39	133
MS.gene060096.t1	MTR_6g045097	42.254	213	108	9	1	199	1	212	1.28e-38	132
MS.gene060096.t1	MTR_6g045403	41.038	212	110	8	1	199	1	210	1.50e-36	127
MS.gene060096.t1	MTR_6g059730	45.581	215	100	10	1	201	1	212	5.76e-36	125
MS.gene060096.t1	MTR_6g009650	41.827	208	102	9	1	194	1	203	9.18e-35	122
MS.gene060096.t1	MTR_6g017225	40.359	223	104	13	1	201	1	216	1.07e-33	123
MS.gene060096.t1	MTR_6g045433	36.681	229	107	10	1	201	1	219	1.66e-32	117
MS.gene060096.t1	MTR_6g066190	39.614	207	106	9	1	193	1	202	2.39e-32	116
MS.gene060096.t1	MTR_6g009563	39.524	210	105	9	1	194	1	204	2.79e-31	113
MS.gene060096.t1	MTR_6g066210	39.070	215	110	10	1	199	1	210	3.00e-29	108
MS.gene060096.t1	MTR_6g059810	37.681	207	103	9	4	190	8	208	2.99e-28	105
MS.gene060096.t1	MTR_1g035770	37.073	205	109	8	6	192	7	209	5.62e-28	105
MS.gene060096.t1	MTR_6g059755	49.074	108	49	4	98	199	18	125	7.04e-25	94.7
MS.gene060096.t1	MTR_6g078070	31.731	208	122	8	1	193	1	203	1.06e-19	83.2
MS.gene060096.t1	MTR_6g078100	32.850	207	123	8	1	193	1	205	3.45e-18	79.3
MS.gene060096.t1	MTR_6g065570	55.932	59	23	1	17	72	15	73	4.15e-17	73.6
MS.gene060096.t1	MTR_0302s0030	29.187	209	115	7	5	190	1	199	2.52e-13	66.2
MS.gene060096.t1	MTR_3g106585	31.313	198	123	9	5	194	6	198	9.13e-13	64.7
MS.gene060096.t1	MTR_0100s0150	28.846	208	116	8	5	190	1	198	1.56e-12	63.9
MS.gene060096.t1	MTR_2g073010	30.151	199	127	7	1	193	6	198	1.76e-11	60.8
MS.gene060096.t1	MTR_6g078290	29.756	205	114	9	11	190	10	209	2.43e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060096.t1	AT1G73260	33.981	206	112	9	5	197	15	209	4.75e-21	87.0
MS.gene060096.t1	AT1G17860	32.044	181	106	8	21	193	24	195	2.61e-15	71.2

Find 48 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTATATTGCACCACTTATC+TGG	0.191488	6.2:-69072998	MS.gene060096:CDS
CAACTGAAAATGATCCCTTT+AGG	0.293355	6.2:-69072578	MS.gene060096:CDS
AACTGAAAATGATCCCTTTA+GGG	0.300397	6.2:-69072577	MS.gene060096:CDS
CCTACACCTTGGGTTGGTAT+TGG	0.305524	6.2:-69072753	MS.gene060096:CDS
GGTAAGTAATGTTGGCTTCA+TGG	0.309270	6.2:+69073118	None:intergenic
GAAACAGTTGAAGACATAAA+TGG	0.316517	6.2:-69073047	MS.gene060096:CDS
AAATCTTGAAGAACAGTAAC+TGG	0.358169	6.2:+69072924	None:intergenic
ACTCGTATGTTAAATCTAAC+TGG	0.363967	6.2:+69072879	None:intergenic
ACTGTTTCAGTAAAAGCTAA+TGG	0.364363	6.2:+69073062	None:intergenic
AAAATTAAGACAATTGGTTC+AGG	0.369696	6.2:-69072699	MS.gene060096:CDS
AATGGAAACCCCGTATTTCC+TGG	0.375075	6.2:-69073029	MS.gene060096:CDS
CCTGGTGGTTGTAGTGATAT+TGG	0.387244	6.2:-69072636	MS.gene060096:CDS
TGATGATTTCCCTACACCTT+GGG	0.392597	6.2:-69072763	MS.gene060096:CDS
TCATTTAAAATTAAGACAAT+TGG	0.397855	6.2:-69072705	MS.gene060096:CDS
TTGGCAGATTCAGCACAGTT+TGG	0.422008	6.2:+69072801	None:intergenic
ATCTGGGGAGCAGGAGGTGG+TGG	0.433696	6.2:-69072981	MS.gene060096:CDS
AGAGAAAAGGTAAGTAATGT+TGG	0.440708	6.2:+69073110	None:intergenic
CTATATTGCACCACTTATCT+GGG	0.458103	6.2:-69072997	MS.gene060096:CDS
GATAATATTGACGCAATTGA+AGG	0.458780	6.2:-69072729	MS.gene060096:CDS
CTGTGCTGAATCTGCCAAAT+GGG	0.460200	6.2:-69072796	MS.gene060096:CDS
ATCATGAGCTAGAACCCATT+TGG	0.471454	6.2:+69072782	None:intergenic
AGATGAGAATGGATGGCGTT+TGG	0.471954	6.2:-69072604	MS.gene060096:CDS
CTGGTGGTTGTAGTGATATT+GGG	0.472823	6.2:-69072635	MS.gene060096:CDS
ACTGTGCTGAATCTGCCAAA+TGG	0.476531	6.2:-69072797	MS.gene060096:CDS
AGTAAAAGCTAATGGAAAGT+AGG	0.481391	6.2:+69073070	None:intergenic
TGGAGATTTGATTCAGACTA+CGG	0.487047	6.2:+69072521	None:intergenic
GGAAAAGAGGAGGAGAGAAA+AGG	0.488375	6.2:+69073097	None:intergenic
GAAAGTAGGCAAAGGAAAAG+AGG	0.491486	6.2:+69073084	None:intergenic
CTTATCTGGGGAGCAGGAGG+TGG	0.493336	6.2:-69072984	MS.gene060096:CDS
GCACCACTTATCTGGGGAGC+AGG	0.501214	6.2:-69072990	MS.gene060096:CDS
ATGATGATTTCCCTACACCT+TGG	0.506844	6.2:-69072764	MS.gene060096:CDS
GATTTCCCTACACCTTGGGT+TGG	0.516772	6.2:-69072759	MS.gene060096:CDS
CCAATACCAACCCAAGGTGT+AGG	0.518662	6.2:+69072753	None:intergenic
GGGAGGTATAGAGATGAGAA+TGG	0.518670	6.2:-69072615	MS.gene060096:CDS
TTTAACATACGAGTATTTCG+TGG	0.521492	6.2:-69072870	MS.gene060096:CDS
CCTCCTGCTCCCCAGATAAG+TGG	0.524330	6.2:+69072987	None:intergenic
GGATTGAAACTTGGTAGGAC+AGG	0.528578	6.2:-69072960	MS.gene060096:CDS
CAATACCAACCCAAGGTGTA+GGG	0.543524	6.2:+69072754	None:intergenic
AGTAGGCAAAGGAAAAGAGG+AGG	0.560366	6.2:+69073087	None:intergenic
GGAGGTGGTGGATTGAAACT+TGG	0.575753	6.2:-69072969	MS.gene060096:CDS
GGTATAGAGATGAGAATGGA+TGG	0.592483	6.2:-69072611	MS.gene060096:CDS
AGCTAATGGAAAGTAGGCAA+AGG	0.597478	6.2:+69073076	None:intergenic
CCACTTATCTGGGGAGCAGG+AGG	0.601917	6.2:-69072987	MS.gene060096:CDS
GTGGTGGATTGAAACTTGGT+AGG	0.627699	6.2:-69072965	MS.gene060096:CDS
CCAATATCACTACAACCACC+AGG	0.635878	6.2:+69072636	None:intergenic
TTATCACCAATACCAACCCA+AGG	0.671863	6.2:+69072747	None:intergenic
TATATTGCACCACTTATCTG+GGG	0.693225	6.2:-69072996	MS.gene060096:CDS
GTGGTTGTAGTGATATTGGG+AGG	0.715809	6.2:-69072632	MS.gene060096:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCATTTAAAATTAAGACAAT+TGG	-	chr6.2:69072944-69072963	MS.gene060096:CDS	15.0%
!!	AAAATTAAGACAATTGGTTC+AGG	-	chr6.2:69072950-69072969	MS.gene060096:CDS	25.0%
	AAATCTTGAAGAACAGTAAC+TGG	+	chr6.2:69072728-69072747	None:intergenic	30.0%
	AACTGAAAATGATCCCTTTA+GGG	-	chr6.2:69073072-69073091	MS.gene060096:CDS	30.0%
	ACTCGTATGTTAAATCTAAC+TGG	+	chr6.2:69072773-69072792	None:intergenic	30.0%
	ACTGTTTCAGTAAAAGCTAA+TGG	+	chr6.2:69072590-69072609	None:intergenic	30.0%
	AGAGAAAAGGTAAGTAATGT+TGG	+	chr6.2:69072542-69072561	None:intergenic	30.0%
	AGTAAAAGCTAATGGAAAGT+AGG	+	chr6.2:69072582-69072601	None:intergenic	30.0%
	ATCAACAAAAACAACCCTAA+AGG	+	chr6.2:69073089-69073108	None:intergenic	30.0%
	GAAACAGTTGAAGACATAAA+TGG	-	chr6.2:69072602-69072621	MS.gene060096:CDS	30.0%
	GATAATATTGACGCAATTGA+AGG	-	chr6.2:69072920-69072939	MS.gene060096:CDS	30.0%
	TCAACAAAAACAACCCTAAA+GGG	+	chr6.2:69073088-69073107	None:intergenic	30.0%
	TTTAACATACGAGTATTTCG+TGG	-	chr6.2:69072779-69072798	MS.gene060096:CDS	30.0%
!	GCAATATAGTATTTTCTACC+AGG	+	chr6.2:69072641-69072660	None:intergenic	30.0%
!	GTATTTTCTACCAGGAAATA+CGG	+	chr6.2:69072633-69072652	None:intergenic	30.0%
!	TAAAGGGATCATTTTCAGTT+GGG	+	chr6.2:69073072-69073091	None:intergenic	30.0%
!	TATTTTCTACCAGGAAATAC+GGG	+	chr6.2:69072632-69072651	None:intergenic	30.0%
	ACTATATTGCACCACTTATC+TGG	-	chr6.2:69072651-69072670	MS.gene060096:CDS	35.0%
	CAACTGAAAATGATCCCTTT+AGG	-	chr6.2:69073071-69073090	MS.gene060096:CDS	35.0%
	CTATATTGCACCACTTATCT+GGG	-	chr6.2:69072652-69072671	MS.gene060096:CDS	35.0%
	TATATTGCACCACTTATCTG+GGG	-	chr6.2:69072653-69072672	MS.gene060096:CDS	35.0%
!	ATTTTCTACCAGGAAATACG+GGG	+	chr6.2:69072631-69072650	None:intergenic	35.0%
!	CTAAAGGGATCATTTTCAGT+TGG	+	chr6.2:69073073-69073092	None:intergenic	35.0%
!	TGTTCTTCAAGATTTTCGTG+AGG	-	chr6.2:69072733-69072752	MS.gene060096:CDS	35.0%
	AGCTAATGGAAAGTAGGCAA+AGG	+	chr6.2:69072576-69072595	None:intergenic	40.0%
	ATCATGAGCTAGAACCCATT+TGG	+	chr6.2:69072870-69072889	None:intergenic	40.0%
	ATGATGATTTCCCTACACCT+TGG	-	chr6.2:69072885-69072904	MS.gene060096:CDS	40.0%
	CCAGGTCGATTACTGAAAAA+TGG	+	chr6.2:69072998-69073017	None:intergenic	40.0%
	CTGGTGGTTGTAGTGATATT+GGG	-	chr6.2:69073014-69073033	MS.gene060096:CDS	40.0%
	GAAAGTAGGCAAAGGAAAAG+AGG	+	chr6.2:69072568-69072587	None:intergenic	40.0%
	GGTATAGAGATGAGAATGGA+TGG	-	chr6.2:69073038-69073057	MS.gene060096:CDS	40.0%
	TGATGATTTCCCTACACCTT+GGG	-	chr6.2:69072886-69072905	MS.gene060096:CDS	40.0%
	TTATCACCAATACCAACCCA+AGG	+	chr6.2:69072905-69072924	None:intergenic	40.0%
!	GATTTTCGTGAGGTTGTTAG+AGG	-	chr6.2:69072743-69072762	MS.gene060096:CDS	40.0%
!!	CCATTTTTCAGTAATCGACC+TGG	-	chr6.2:69072995-69073014	MS.gene060096:CDS	40.0%
!!	TTTTTCAGTAATCGACCTGG+TGG	-	chr6.2:69072998-69073017	MS.gene060096:CDS	40.0%
	AATGGAAACCCCGTATTTCC+TGG	-	chr6.2:69072620-69072639	MS.gene060096:CDS	45.0%
	ACTGTGCTGAATCTGCCAAA+TGG	-	chr6.2:69072852-69072871	MS.gene060096:CDS	45.0%
	AGATGAGAATGGATGGCGTT+TGG	-	chr6.2:69073045-69073064	MS.gene060096:CDS	45.0%
	AGTAGGCAAAGGAAAAGAGG+AGG	+	chr6.2:69072565-69072584	None:intergenic	45.0%
	CAATACCAACCCAAGGTGTA+GGG	+	chr6.2:69072898-69072917	None:intergenic	45.0%
	CCAATATCACTACAACCACC+AGG	+	chr6.2:69073016-69073035	None:intergenic	45.0%
	CCTGGTGGTTGTAGTGATAT+TGG	-	chr6.2:69073013-69073032	MS.gene060096:CDS	45.0%
	CTGTGCTGAATCTGCCAAAT+GGG	-	chr6.2:69072853-69072872	MS.gene060096:CDS	45.0%
	GGAAAAGAGGAGGAGAGAAA+AGG	+	chr6.2:69072555-69072574	None:intergenic	45.0%
	GGATTGAAACTTGGTAGGAC+AGG	-	chr6.2:69072689-69072708	MS.gene060096:CDS	45.0%
	GTGGTTGTAGTGATATTGGG+AGG	-	chr6.2:69073017-69073036	MS.gene060096:CDS	45.0%
	TTGGCAGATTCAGCACAGTT+TGG	+	chr6.2:69072851-69072870	None:intergenic	45.0%
!	GGGAGGTATAGAGATGAGAA+TGG	-	chr6.2:69073034-69073053	MS.gene060096:CDS	45.0%
!!	GTGGTGGATTGAAACTTGGT+AGG	-	chr6.2:69072684-69072703	MS.gene060096:CDS	45.0%
	CCAATACCAACCCAAGGTGT+AGG	+	chr6.2:69072899-69072918	None:intergenic	50.0%
	GATTTCCCTACACCTTGGGT+TGG	-	chr6.2:69072890-69072909	MS.gene060096:CDS	50.0%
!!	CCTACACCTTGGGTTGGTAT+TGG	-	chr6.2:69072896-69072915	MS.gene060096:CDS	50.0%
!!	GGAGGTGGTGGATTGAAACT+TGG	-	chr6.2:69072680-69072699	MS.gene060096:CDS	50.0%
	CCACTTATCTGGGGAGCAGG+AGG	-	chr6.2:69072662-69072681	MS.gene060096:CDS	60.0%
	CCTCCTGCTCCCCAGATAAG+TGG	+	chr6.2:69072665-69072684	None:intergenic	60.0%
	CTTATCTGGGGAGCAGGAGG+TGG	-	chr6.2:69072665-69072684	MS.gene060096:CDS	60.0%
	GCACCACTTATCTGGGGAGC+AGG	-	chr6.2:69072659-69072678	MS.gene060096:CDS	60.0%
	ATCTGGGGAGCAGGAGGTGG+TGG	-	chr6.2:69072668-69072687	MS.gene060096:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	69072533	69073138	69072533	ID=MS.gene060096
chr6.2	mRNA	69072533	69073138	69072533	ID=MS.gene060096.t1;Parent=MS.gene060096
chr6.2	exon	69072533	69073138	69072533	ID=MS.gene060096.t1.exon1;Parent=MS.gene060096.t1
chr6.2	CDS	69072533	69073138	69072533	ID=cds.MS.gene060096.t1;Parent=MS.gene060096.t1

Gene Sequence

>MS.gene060096

ATGAAGCCAACATTACTTACCTTTTCTCTCCTCCTCTTTTCCTTTGCCTACTTTCCATTAGCTTTTACTGAAACAGTTGAAGACATAAATGGAAACCCCGTATTTCCTGGTAGAAAATACTATATTGCACCACTTATCTGGGGAGCAGGAGGTGGTGGATTGAAACTTGGTAGGACAGGAAACTCAGAATGTCCAGTTACTGTTCTTCAAGATTTTCGTGAGGTTGTTAGAGGCTTGCCAGTTAGATTTAACATACGAGTATTTCGTGGTGTTATCTTTACAACTGACGAAGTAGATATTGAGTTTGTTAAGAAACCAAACTGTGCTGAATCTGCCAAATGGGTTCTAGCTCATGATGATTTCCCTACACCTTGGGTTGGTATTGGTGATAATATTGACGCAATTGAAGGTTCATTTAAAATTAAGACAATTGGTTCAGGAGCATACAAGCTTGTGTATTGTCCATTTTTCAGTAATCGACCTGGTGGTTGTAGTGATATTGGGAGGTATAGAGATGAGAATGGATGGCGTTTGGTCCCAACTGAAAATGATCCCTTTAGGGTTGTTTTTGTTGATGCTAATGAATCTGAAAAAGCCGTAGTCTGA

Protein sequence

>MS.gene060096.t1

MKPTLLTFSLLLFSFAYFPLAFTETVEDINGNPVFPGRKYYIAPLIWGAGGGGLKLGRTGNSECPVTVLQDFREVVRGLPVRFNIRVFRGVIFTTDEVDIEFVKKPNCAESAKWVLAHDDFPTPWVGIGDNIDAIEGSFKIKTIGSGAYKLVYCPFFSNRPGGCSDIGRYRDENGWRLVPTENDPFRVVFVDANESEKAVV