AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67889


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTTAATTTAGAATATT+TGG 0.125218 2.2:-55261186 None:intergenic
TGTACCCAGTTTGGCCATTT+TGG 0.134807 2.2:+55261073 None:intergenic
CAGCTAGTTGCAGGCCTTCT+AGG 0.184241 2.2:-55260304 MS.gene67889:CDS
AAGGATATTATCTTGCAATT+TGG 0.237243 2.2:-55261044 MS.gene67889:CDS
CAAACTGGGTACATTTATTA+AGG 0.242603 2.2:-55261063 MS.gene67889:CDS
CCCACCTATTGGGGCCAAAA+TGG 0.283148 2.2:-55261087 MS.gene67889:CDS
TGGGGCCAAAATGGCCAAAC+TGG 0.309884 2.2:-55261078 MS.gene67889:CDS
TCAGCGCTTAGGAATGAATT+AGG 0.319413 2.2:-55260175 MS.gene67889:CDS
CTTAATAAATGTACCCAGTT+TGG 0.325391 2.2:+55261064 None:intergenic
ATTCCTTATGTCGGTCTTAC+CGG 0.330047 2.2:-55260696 MS.gene67889:CDS
GAAATATTGAGAATTGTATA+TGG 0.347955 2.2:-55260067 MS.gene67889:CDS
ACAATTCTCAATATTTCAAT+TGG 0.353451 2.2:+55260073 None:intergenic
ATCTCAGGAAATATCCTTGC+TGG 0.373800 2.2:-55260127 MS.gene67889:CDS
CAATTTGGTTTGCAAGAAGT+TGG 0.389960 2.2:-55261029 MS.gene67889:CDS
GCATGGAAGGTGTATCCTTA+TGG 0.391268 2.2:-55260229 MS.gene67889:CDS
TGGATTGGATGTAGTTGATT+CGG 0.408733 2.2:-55261129 MS.gene67889:CDS
CTTAGATCCATACATGGCCT+TGG 0.431043 2.2:+55260825 None:intergenic
AAGTTGACATCATTTGCATA+TGG 0.448703 2.2:+55260738 None:intergenic
GGGGTTCCCAAGGCCATGTA+TGG 0.457536 2.2:-55260832 MS.gene67889:CDS
GGCCTTCTAGGAGTGGAATC+AGG 0.458196 2.2:-55260292 MS.gene67889:CDS
GGCCTGATTCCACTCCTAGA+AGG 0.458732 2.2:+55260290 None:intergenic
TCATTTGCATATGGATCAAA+AGG 0.461244 2.2:+55260747 None:intergenic
AGCAAGGATATTTCCTGAGA+TGG 0.467561 2.2:+55260129 None:intergenic
AAGGTGATGGATAGTGCCTT+TGG 0.473626 2.2:-55260786 MS.gene67889:CDS
TGCAAGAAGTTGGACATTTG+AGG 0.477314 2.2:-55261019 MS.gene67889:intron
TGGATCATTAGGTCATGGAT+TGG 0.491581 2.2:-55261144 MS.gene67889:CDS
TCCTACAAAATGCTACTTCG+AGG 0.497607 2.2:-55260653 MS.gene67889:CDS
GTCGGTCTTACCGGCTATGT+TGG 0.505513 2.2:-55260687 MS.gene67889:CDS
TCTGCCACAGTTACTCCATA+AGG 0.507698 2.2:+55260214 None:intergenic
TTAGATCCATACATGGCCTT+GGG 0.510288 2.2:+55260826 None:intergenic
CTAATCGCATCTCAGCGCTT+AGG 0.514135 2.2:-55260186 MS.gene67889:CDS
GCTATAAAGAGCACAGTGAA+GGG 0.515388 2.2:-55260852 MS.gene67889:CDS
ATCATCATTTGCCAAGGTGA+TGG 0.516251 2.2:-55260799 MS.gene67889:CDS
CCTCTAGGGTAGAAGCCACC+TGG 0.517093 2.2:+55260025 None:intergenic
ATAACACCATCTCCTCCTCT+AGG 0.519512 2.2:+55260010 None:intergenic
ATAAAAGATGAACCCATCTC+AGG 0.525491 2.2:-55260142 MS.gene67889:CDS
GGCTATAAAGAGCACAGTGA+AGG 0.527779 2.2:-55260853 MS.gene67889:CDS
GCAAGGATATTTCCTGAGAT+GGG 0.529908 2.2:+55260130 None:intergenic
GGATCAAAAGGTGGGTGTAG+AGG 0.531869 2.2:+55260759 None:intergenic
TTATTTGGATCATTAGGTCA+TGG 0.533706 2.2:-55261149 MS.gene67889:CDS
TGTTATACGAACGACGTGCA+TGG 0.542914 2.2:-55260246 MS.gene67889:CDS
TGGATTGGTGCCAACATAGC+CGG 0.545039 2.2:+55260677 None:intergenic
TTGAGAATTGTATATGGAAG+TGG 0.548661 2.2:-55260061 MS.gene67889:CDS
GTTGATTCGGATTTAGCAGA+GGG 0.554995 2.2:-55261116 MS.gene67889:CDS
ATACGAACGACGTGCATGGA+AGG 0.556244 2.2:-55260242 MS.gene67889:CDS
TTCTACCCTAGAGGAGGAGA+TGG 0.561993 2.2:-55260016 MS.gene67889:CDS
ATGATCGTATAACTGCATCT+TGG 0.571142 2.2:+55260269 None:intergenic
TAGCCGGTAAGACCGACATA+AGG 0.572610 2.2:+55260693 None:intergenic
CTCTAGGGTAGAAGCCACCT+GGG 0.572783 2.2:+55260026 None:intergenic
GATTCGGATTTAGCAGAGGG+TGG 0.575302 2.2:-55261113 MS.gene67889:CDS
TCTTATGTGATTCCTTATGT+CGG 0.575588 2.2:-55260705 MS.gene67889:CDS
TTTGCATATGGATCAAAAGG+TGG 0.585853 2.2:+55260750 None:intergenic
AGTGGTGATGAACATGTCCC+AGG 0.596378 2.2:-55260043 MS.gene67889:CDS
TAACACCATCTCCTCCTCTA+GGG 0.597219 2.2:+55260011 None:intergenic
ACAAAATGCTACTTCGAGGA+AGG 0.597559 2.2:-55260649 MS.gene67889:intron
CCAGGTGGCTTCTACCCTAG+AGG 0.599664 2.2:-55260025 MS.gene67889:CDS
GCACAGTGAAGGGGTTCCCA+AGG 0.602015 2.2:-55260842 MS.gene67889:CDS
AGTTGCAGGCCTTCTAGGAG+TGG 0.603562 2.2:-55260299 MS.gene67889:CDS
GGGGCCAAAATGGCCAAACT+GGG 0.606123 2.2:-55261077 MS.gene67889:CDS
TTGCATATGGATCAAAAGGT+GGG 0.620461 2.2:+55260751 None:intergenic
AAGCTCATCATCATTTGCCA+AGG 0.621976 2.2:-55260805 MS.gene67889:CDS
AAAGGCACTATCCATCACCT+TGG 0.631525 2.2:+55260788 None:intergenic
AGGAATGAATTAGGAAACGA+TGG 0.646376 2.2:-55260166 MS.gene67889:CDS
GATGAGCTTAGATCCATACA+TGG 0.648355 2.2:+55260819 None:intergenic
AGTTGATTCGGATTTAGCAG+AGG 0.651747 2.2:-55261117 MS.gene67889:CDS
GGTGGCTTCTACCCTAGAGG+AGG 0.663458 2.2:-55260022 MS.gene67889:CDS
CTATAAAGAGCACAGTGAAG+GGG 0.666124 2.2:-55260851 MS.gene67889:CDS
GTATCCTTATGGAGTAACTG+TGG 0.688628 2.2:-55260218 MS.gene67889:CDS
GGTGATGAACATGTCCCAGG+TGG 0.767815 2.2:-55260040 MS.gene67889:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTAAAATTATTTTAGT+AGG + chr2.2:55260817-55260836 None:intergenic 10.0%
!!! TTGTATGAATCTTTATTTTA+GGG - chr2.2:55260280-55260299 MS.gene67889:CDS 15.0%
!! ACAATTCTCAATATTTCAAT+TGG + chr2.2:55261084-55261103 None:intergenic 20.0%
!! GAAATATTGAGAATTGTATA+TGG - chr2.2:55261087-55261106 MS.gene67889:CDS 20.0%
!! GTTTACAAAACTAACTTAAA+AGG + chr2.2:55260735-55260754 None:intergenic 20.0%
!!! ACTTGTTAGTAATAAAAGAA+TGG - chr2.2:55260590-55260609 MS.gene67889:intron 20.0%
!!! ATGATAAAAAAAGTACTAGA+GGG + chr2.2:55260163-55260182 None:intergenic 20.0%
!!! GTTGTATGAATCTTTATTTT+AGG - chr2.2:55260279-55260298 MS.gene67889:CDS 20.0%
!!! GTTTTACTCGAAAATAAATA+AGG - chr2.2:55260684-55260703 MS.gene67889:CDS 20.0%
!!! TTCTTTTTATTTGGATCATT+AGG - chr2.2:55259999-55260018 MS.gene67889:CDS 20.0%
! AAGGATATTATCTTGCAATT+TGG - chr2.2:55260110-55260129 MS.gene67889:CDS 25.0%
!! GAAGCTGAATTCTTTTTATT+TGG - chr2.2:55259990-55260009 MS.gene67889:CDS 25.0%
!!! ATTCACTTGCGATTTTATTT+AGG - chr2.2:55260651-55260670 MS.gene67889:CDS 25.0%
!!! GATGATAAAAAAAGTACTAG+AGG + chr2.2:55260164-55260183 None:intergenic 25.0%
AAGTTGACATCATTTGCATA+TGG + chr2.2:55260419-55260438 None:intergenic 30.0%
CAAACTGGGTACATTTATTA+AGG - chr2.2:55260091-55260110 MS.gene67889:CDS 30.0%
CTTAATAAATGTACCCAGTT+TGG + chr2.2:55260093-55260112 None:intergenic 30.0%
TCATTTGCATATGGATCAAA+AGG + chr2.2:55260410-55260429 None:intergenic 30.0%
TCTTATGTGATTCCTTATGT+CGG - chr2.2:55260449-55260468 MS.gene67889:intron 30.0%
TTATTTGGATCATTAGGTCA+TGG - chr2.2:55260005-55260024 MS.gene67889:CDS 30.0%
TTGAGAATTGTATATGGAAG+TGG - chr2.2:55261093-55261112 MS.gene67889:CDS 30.0%
AGGAATGAATTAGGAAACGA+TGG - chr2.2:55260988-55261007 MS.gene67889:intron 35.0%
ATAAAAGATGAACCCATCTC+AGG - chr2.2:55261012-55261031 MS.gene67889:intron 35.0%
ATGATCGTATAACTGCATCT+TGG + chr2.2:55260888-55260907 None:intergenic 35.0%
TTGCATATGGATCAAAAGGT+GGG + chr2.2:55260406-55260425 None:intergenic 35.0%
TTTGCATATGGATCAAAAGG+TGG + chr2.2:55260407-55260426 None:intergenic 35.0%
! TGGATTGGATGTAGTTGATT+CGG - chr2.2:55260025-55260044 MS.gene67889:CDS 35.0%
!! CAATTTGGTTTGCAAGAAGT+TGG - chr2.2:55260125-55260144 MS.gene67889:CDS 35.0%
!! GAATTTTAACAGCTAGTTGC+AGG - chr2.2:55260841-55260860 MS.gene67889:CDS 35.0%
AAGCTCATCATCATTTGCCA+AGG - chr2.2:55260349-55260368 MS.gene67889:intron 40.0%
AGCAAGGATATTTCCTGAGA+TGG + chr2.2:55261028-55261047 None:intergenic 40.0%
AGTGAATAACACGCGTCAAT+CGG + chr2.2:55260638-55260657 None:intergenic 40.0%
ATCATCATTTGCCAAGGTGA+TGG - chr2.2:55260355-55260374 MS.gene67889:intron 40.0%
ATCTCAGGAAATATCCTTGC+TGG - chr2.2:55261027-55261046 MS.gene67889:CDS 40.0%
ATTCCTTATGTCGGTCTTAC+CGG - chr2.2:55260458-55260477 MS.gene67889:intron 40.0%
GATGAGCTTAGATCCATACA+TGG + chr2.2:55260338-55260357 None:intergenic 40.0%
GCAAGGATATTTCCTGAGAT+GGG + chr2.2:55261027-55261046 None:intergenic 40.0%
GTATCCTTATGGAGTAACTG+TGG - chr2.2:55260936-55260955 MS.gene67889:intron 40.0%
TCAGCGCTTAGGAATGAATT+AGG - chr2.2:55260979-55260998 MS.gene67889:intron 40.0%
TGCAAGAAGTTGGACATTTG+AGG - chr2.2:55260135-55260154 MS.gene67889:CDS 40.0%
TGGATCATTAGGTCATGGAT+TGG - chr2.2:55260010-55260029 MS.gene67889:CDS 40.0%
TTAGATCCATACATGGCCTT+GGG + chr2.2:55260331-55260350 None:intergenic 40.0%
! ACAAAATGCTACTTCGAGGA+AGG - chr2.2:55260505-55260524 MS.gene67889:intron 40.0%
! AGCATTTTGTAGGAGTGGAT+TGG + chr2.2:55260495-55260514 None:intergenic 40.0%
! CTATAAAGAGCACAGTGAAG+GGG - chr2.2:55260303-55260322 MS.gene67889:CDS 40.0%
! GAAGTAGCATTTTGTAGGAG+TGG + chr2.2:55260500-55260519 None:intergenic 40.0%
! GCTATAAAGAGCACAGTGAA+GGG - chr2.2:55260302-55260321 MS.gene67889:CDS 40.0%
! GTGAATTTTCATCACCAGCA+AGG + chr2.2:55261044-55261063 None:intergenic 40.0%
! TCCTACAAAATGCTACTTCG+AGG - chr2.2:55260501-55260520 MS.gene67889:intron 40.0%
! TCCTCGAAGTAGCATTTTGT+AGG + chr2.2:55260505-55260524 None:intergenic 40.0%
!! AGTTGATTCGGATTTAGCAG+AGG - chr2.2:55260037-55260056 MS.gene67889:CDS 40.0%
!! GTTGATTCGGATTTAGCAGA+GGG - chr2.2:55260038-55260057 MS.gene67889:CDS 40.0%
AGTAGGTCGCGATGAAATCA+TGG + chr2.2:55260800-55260819 None:intergenic 45.0%
ATAACACCATCTCCTCCTCT+AGG + chr2.2:55261147-55261166 None:intergenic 45.0%
CTTAGATCCATACATGGCCT+TGG + chr2.2:55260332-55260351 None:intergenic 45.0%
GTGAATAACACGCGTCAATC+GGG + chr2.2:55260637-55260656 None:intergenic 45.0%
TAACACCATCTCCTCCTCTA+GGG + chr2.2:55261146-55261165 None:intergenic 45.0%
TCTGCCACAGTTACTCCATA+AGG + chr2.2:55260943-55260962 None:intergenic 45.0%
TGTTATACGAACGACGTGCA+TGG - chr2.2:55260908-55260927 MS.gene67889:intron 45.0%
! GCATGGAAGGTGTATCCTTA+TGG - chr2.2:55260925-55260944 MS.gene67889:intron 45.0%
! GGCTATAAAGAGCACAGTGA+AGG - chr2.2:55260301-55260320 MS.gene67889:CDS 45.0%
! TGGGTGTAGAGGTTTTCCAA+AGG + chr2.2:55260387-55260406 None:intergenic 45.0%
! TGTACCCAGTTTGGCCATTT+TGG + chr2.2:55260084-55260103 None:intergenic 45.0%
!! AAAGGCACTATCCATCACCT+TGG + chr2.2:55260369-55260388 None:intergenic 45.0%
!! AAGGTGATGGATAGTGCCTT+TGG - chr2.2:55260368-55260387 MS.gene67889:intron 45.0%
ATACGAACGACGTGCATGGA+AGG - chr2.2:55260912-55260931 MS.gene67889:intron 50.0%
CTAATCGCATCTCAGCGCTT+AGG - chr2.2:55260968-55260987 MS.gene67889:intron 50.0%
GATTCGGATTTAGCAGAGGG+TGG - chr2.2:55260041-55260060 MS.gene67889:CDS 50.0%
GGATCAAAAGGTGGGTGTAG+AGG + chr2.2:55260398-55260417 None:intergenic 50.0%
TAGCCGGTAAGACCGACATA+AGG + chr2.2:55260464-55260483 None:intergenic 50.0%
TTCTACCCTAGAGGAGGAGA+TGG - chr2.2:55261138-55261157 MS.gene67889:CDS 50.0%
! AGTGGTGATGAACATGTCCC+AGG - chr2.2:55261111-55261130 MS.gene67889:CDS 50.0%
!! ATTTTGGCCCCAATAGGTGG+GGG + chr2.2:55260068-55260087 None:intergenic 50.0%
!! CATTTTGGCCCCAATAGGTG+GGG + chr2.2:55260069-55260088 None:intergenic 50.0%
!! CCATTTTGGCCCCAATAGGT+GGG + chr2.2:55260070-55260089 None:intergenic 50.0%
!! TGGATTGGTGCCAACATAGC+CGG + chr2.2:55260480-55260499 None:intergenic 50.0%
!! TTGGCCATTTTGGCCCCAAT+AGG + chr2.2:55260074-55260093 None:intergenic 50.0%
CAGCTAGTTGCAGGCCTTCT+AGG - chr2.2:55260850-55260869 MS.gene67889:CDS 55.0%
CCCACCTATTGGGGCCAAAA+TGG - chr2.2:55260067-55260086 MS.gene67889:CDS 55.0%
GGCCTGATTCCACTCCTAGA+AGG + chr2.2:55260867-55260886 None:intergenic 55.0%
GGGGCCAAAATGGCCAAACT+GGG - chr2.2:55260077-55260096 MS.gene67889:CDS 55.0%
GGTGATGAACATGTCCCAGG+TGG - chr2.2:55261114-55261133 MS.gene67889:CDS 55.0%
TGGGGCCAAAATGGCCAAAC+TGG - chr2.2:55260076-55260095 MS.gene67889:CDS 55.0%
! AGTTGCAGGCCTTCTAGGAG+TGG - chr2.2:55260855-55260874 MS.gene67889:CDS 55.0%
! GGCCTTCTAGGAGTGGAATC+AGG - chr2.2:55260862-55260881 MS.gene67889:intron 55.0%
! GTCGGTCTTACCGGCTATGT+TGG - chr2.2:55260467-55260486 MS.gene67889:intron 55.0%
!! CTCTAGGGTAGAAGCCACCT+GGG + chr2.2:55261131-55261150 None:intergenic 55.0%
!! GCCATTTTGGCCCCAATAGG+TGG + chr2.2:55260071-55260090 None:intergenic 55.0%
AGGGTGGACCCCCACCTATT+GGG - chr2.2:55260057-55260076 MS.gene67889:CDS 60.0%
CCAGGTGGCTTCTACCCTAG+AGG - chr2.2:55261129-55261148 MS.gene67889:CDS 60.0%
GCACAGTGAAGGGGTTCCCA+AGG - chr2.2:55260312-55260331 MS.gene67889:intron 60.0%
GGGGTTCCCAAGGCCATGTA+TGG - chr2.2:55260322-55260341 MS.gene67889:intron 60.0%
GGTGGCTTCTACCCTAGAGG+AGG - chr2.2:55261132-55261151 MS.gene67889:CDS 60.0%
!! CCTCTAGGGTAGAAGCCACC+TGG + chr2.2:55261132-55261151 None:intergenic 60.0%
GAGGGTGGACCCCCACCTAT+TGG - chr2.2:55260056-55260075 MS.gene67889:CDS 65.0%
GGGTGGACCCCCACCTATTG+GGG - chr2.2:55260058-55260077 MS.gene67889:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 55259982 55261194 55259982 ID=MS.gene67889
chr2.2 mRNA 55259982 55261194 55259982 ID=MS.gene67889.t1;Parent=MS.gene67889
chr2.2 exon 55261020 55261194 55261020 ID=MS.gene67889.t1.exon1;Parent=MS.gene67889.t1
chr2.2 CDS 55261020 55261194 55261020 ID=cds.MS.gene67889.t1;Parent=MS.gene67889.t1
chr2.2 exon 55260650 55260875 55260650 ID=MS.gene67889.t1.exon2;Parent=MS.gene67889.t1
chr2.2 CDS 55260650 55260875 55260650 ID=cds.MS.gene67889.t1;Parent=MS.gene67889.t1
chr2.2 exon 55259982 55260323 55259982 ID=MS.gene67889.t1.exon3;Parent=MS.gene67889.t1
chr2.2 CDS 55259982 55260323 55259982 ID=cds.MS.gene67889.t1;Parent=MS.gene67889.t1
Gene Sequence

>MS.gene67889

AATATTTGGAAGCTGAATTCTTTTTATTTGGATCATTAGGTCATGGATTGGATGTAGTTGATTCGGATTTAGCAGAGGGTGGACCCCCACCTATTGGGGCCAAAATGGCCAAACTGGGTACATTTATTAAGGATATTATCTTGCAATTTGGTTTGCAAGAAGTTGGACATTTGAGGTACCCTCTAGTACTTTTTTTATCATCATCTCTTTCGTACTTTTTGAATCTTATAATTTGACATATATAGTGTTTTATTATGTCAATATATCTTGAGAGTCAAGTTTATTGTTTAAAGTGAAGTTGTATGAATCTTTATTTTAGGGCTATAAAGAGCACAGTGAAGGGGTTCCCAAGGCCATGTATGGATCTAAGCTCATCATCATTTGCCAAGGTGATGGATAGTGCCTTTGGAAAACCTCTACACCCACCTTTTGATCCATATGCAAATGATGTCAACTTTCTGCTTGCATCTTATGTGATTCCTTATGTCGGTCTTACCGGCTATGTTGGCACCAATCCACTCCTACAAAATGCTACTTCGAGGAAGGTATATCATCATACTCATCATATTAAAACTTTTCAAAACTATAGAAAAATCGATCAAGCATGTACTTGTTAGTAATAAAAGAATGGCACAATTCTATTTAAAAAATACCCGATTGACGCGTGTTATTCACTTGCGATTTTATTTAGGAAAATTTCAAGTTTTACTCGAAAATAAATAAGGCTGAAAATAGTTTATTAATAGATCACCTTTTAAGTTAGTTTTGTAAACATTAGTTAGACTCGATATAAAAAACTTCATAATTTTTTTTTACCATGATTTCATCGCGACCTACTAAAATAATTTTAATGTTGTGTGAATTTTAACAGCTAGTTGCAGGCCTTCTAGGAGTGGAATCAGGCCAAGATGCAGTTATACGATCATTGTTATACGAACGACGTGCATGGAAGGTGTATCCTTATGGAGTAACTGTGGCAGAGTTCACTAATCGCATCTCAGCGCTTAGGAATGAATTAGGAAACGATGGTATAAAAGATGAACCCATCTCAGGAAATATCCTTGCTGGTGATGAAAATTCACTATCATATTCAAGAACTCCAATTGAAATATTGAGAATTGTATATGGAAGTGGTGATGAACATGTCCCAGGTGGCTTCTACCCTAGAGGAGGAGATGGTGTTATAGCAAAATCTTACTTAGTACTTAATTAG

Protein sequence

>MS.gene67889.t1

NIWKLNSFYLDHUVMDWMULIRIUQRVDPHLLGPKWPNWVHLLRILSCNLVCKKLDIUGLURAQURGSQGHVWIUAHHHLPRUWIVPLENLYTHLLIHMQMMSTFCLHLMUFLMSVLPAMLAPIHSYKMLLRGSULQAFUEWNQAKMQLYDHCYTNDVHGRCILMEULWQSSLIASQRLGMNUETMVUKMNPSQEISLLVMKIHYHIQELQLKYUELYMEVVMNMSQVASTLEEEMVLUQNLTUYLI