AlfalfaGEDB | MS.gene063143

M. sativa cultivar XinJiangDaYe / MS.gene063143

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063143.t1	XP_003603971.2	96.8	156	4	1	1	156	1	155	1.40E-69	272.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063143.t1	C0Z2L5	44.2	156	79	3	2	149	9	164	1.1e-19	97.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063143.t1	G7J773	96.8	156	4	1	1	156	1	155	9.9e-70	272.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene063143.t1	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene063143	0.817084	3.96E-52	-1.69E-46
MS.gene049499	MS.gene063143	0.803256	3.80E-49	-1.69E-46
MS.gene050945	MS.gene063143	0.824013	1.02E-53	-1.69E-46
MS.gene051234	MS.gene063143	0.813254	2.81E-51	-1.69E-46
MS.gene051365	MS.gene063143	0.80373	3.03E-49	-1.69E-46
MS.gene052533	MS.gene063143	0.832855	7.45E-56	-1.69E-46
MS.gene054142	MS.gene063143	0.806444	8.20E-50	-1.69E-46
MS.gene054413	MS.gene063143	0.802979	4.34E-49	-1.69E-46
MS.gene054957	MS.gene063143	0.810507	1.11E-50	-1.69E-46
MS.gene055513	MS.gene063143	0.817458	3.26E-52	-1.69E-46
MS.gene05689	MS.gene063143	0.81427	1.68E-51	-1.69E-46
MS.gene057102	MS.gene063143	0.839842	1.24E-57	-1.69E-46
MS.gene058781	MS.gene063143	0.804095	2.55E-49	-1.69E-46
MS.gene059146	MS.gene063143	0.809739	1.63E-50	-1.69E-46
MS.gene05985	MS.gene063143	-0.833663	4.69E-56	-1.69E-46
MS.gene059901	MS.gene063143	-0.807188	5.71E-50	-1.69E-46
MS.gene059935	MS.gene063143	0.809225	2.10E-50	-1.69E-46
MS.gene060338	MS.gene063143	0.808445	3.09E-50	-1.69E-46
MS.gene060365	MS.gene063143	0.804926	1.71E-49	-1.69E-46
MS.gene060763	MS.gene063143	0.828655	7.97E-55	-1.69E-46
MS.gene060830	MS.gene063143	0.800037	1.74E-48	-1.69E-46
MS.gene060933	MS.gene063143	0.816829	4.52E-52	-1.69E-46
MS.gene060935	MS.gene063143	0.830332	3.12E-55	-1.69E-46
MS.gene060937	MS.gene063143	0.84951	3.09E-60	-1.69E-46
MS.gene061188	MS.gene063143	0.811534	6.67E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063143.t1	MTR_3g117120	94.872	156	7	1	1	156	1	155	1.48e-89	258
MS.gene063143.t1	MTR_4g070860	71.242	153	42	1	1	151	1	153	3.46e-63	191
MS.gene063143.t1	MTR_1g022495	60.976	164	48	4	1	153	1	159	5.72e-57	175
MS.gene063143.t1	MTR_8g086020	53.086	162	68	5	1	156	1	160	1.04e-45	147
MS.gene063143.t1	MTR_3g112220	63.291	79	29	0	27	105	1	79	3.83e-29	102
MS.gene063143.t1	MTR_1g087040	39.073	151	88	2	1	151	1	147	7.64e-26	96.3
MS.gene063143.t1	MTR_6g016375	44.118	102	47	2	26	124	86	180	3.96e-18	77.4
MS.gene063143.t1	MTR_8g015250	50.685	73	36	0	26	98	70	142	3.31e-17	74.7
MS.gene063143.t1	MTR_7g029400	44.444	99	51	2	1	98	57	152	3.81e-17	74.7
MS.gene063143.t1	MTR_7g017880	54.795	73	33	0	26	98	83	155	6.34e-17	74.3
MS.gene063143.t1	MTR_4g097440	39.091	110	60	1	7	109	45	154	1.27e-15	70.5
MS.gene063143.t1	MTR_5g015090	41.053	95	48	1	27	121	58	144	3.63e-14	66.2
MS.gene063143.t1	MTR_3g109310	34.259	108	67	1	6	109	98	205	1.56e-12	63.9
MS.gene063143.t1	MTR_7g109510	53.012	83	39	0	27	109	19	101	5.77e-12	60.1
MS.gene063143.t1	MTR_8g070820	36.735	98	58	2	24	118	300	396	3.95e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063143.t1	AT1G75390	51.829	164	55	5	2	149	9	164	9.74e-48	152
MS.gene063143.t1	AT4G34590	57.143	133	49	2	26	150	23	155	5.68e-44	142
MS.gene063143.t1	AT2G18160	47.020	151	58	5	22	153	23	170	1.25e-36	124
MS.gene063143.t1	AT1G75390	60.825	97	34	2	2	94	9	105	1.02e-32	112
MS.gene063143.t1	AT3G62420	44.604	139	74	2	13	151	8	143	4.59e-30	107
MS.gene063143.t1	AT2G04038	48.837	86	41	1	16	98	57	142	4.97e-18	76.6
MS.gene063143.t1	AT5G15830	34.459	148	79	3	9	156	55	184	1.86e-17	75.5
MS.gene063143.t1	AT1G13600	45.361	97	48	2	16	107	70	166	1.00e-16	73.9
MS.gene063143.t1	AT3G30530	52.055	73	35	0	26	98	77	149	2.61e-16	72.4
MS.gene063143.t1	AT5G38800	47.500	80	42	0	19	98	61	140	1.55e-15	70.1
MS.gene063143.t1	AT5G49450	36.190	105	64	1	27	128	13	117	1.59e-12	61.6
MS.gene063143.t1	AT4G37730	45.000	80	37	1	26	98	193	272	5.43e-12	62.4
MS.gene063143.t1	AT2G22850	37.079	89	56	0	23	111	122	210	2.60e-11	59.7
MS.gene063143.t1	AT2G22850	37.079	89	56	0	23	111	122	210	2.60e-11	59.7
MS.gene063143.t1	AT3G49760	55.814	43	19	0	28	70	70	112	4.56e-11	58.2
MS.gene063143.t1	AT1G68880	38.554	83	51	0	12	94	29	111	4.60e-11	57.8

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCCTCTGAACCTGAATT+TGG	0.134136	3.4:-99603676	None:intergenic
CTTCAATAACCCTTTGAATA+TGG	0.156253	3.4:+99604026	MS.gene063143:CDS
CCTCACAACTCAACGATTCT+TGG	0.233985	3.4:+99603858	MS.gene063143:CDS
TGTTCAACACTTCCAAATTC+AGG	0.300497	3.4:+99603664	MS.gene063143:CDS
TCTTCAAGTGGAACATCTTC+AGG	0.306987	3.4:+99603640	MS.gene063143:CDS
TTCAATAACCCTTTGAATAT+GGG	0.313680	3.4:+99604027	MS.gene063143:CDS
TTTGGATGATCTTGCTGTTC+AGG	0.339403	3.4:+99603789	MS.gene063143:CDS
TTATTTGAACCAACCAATCA+TGG	0.433242	3.4:+99604050	MS.gene063143:CDS
TTCAAATAACCCATATTCAA+AGG	0.450188	3.4:-99604036	None:intergenic
GAGATCTTCTTGCTGATTCG+CGG	0.468319	3.4:-99603747	None:intergenic
ATATCTGCAGAAGCCATGAT+TGG	0.469872	3.4:-99604063	None:intergenic
GGAAGATTTGATGCTTCTGA+TGG	0.470481	3.4:+99603693	MS.gene063143:CDS
TCAAATAACCCATATTCAAA+GGG	0.472163	3.4:-99604035	None:intergenic
CTGTTCAGGTGAGTCAACTA+AGG	0.511341	3.4:+99603803	MS.gene063143:CDS
CTGCAGAAGCCATGATTGGT+TGG	0.535667	3.4:-99604059	None:intergenic
AACTTCATGAATGTTGCAAA+TGG	0.546215	3.4:+99603961	MS.gene063143:CDS
TCTCACATGGCTTCTTCAAG+TGG	0.568523	3.4:+99603628	None:intergenic
ACTTCCAAATTCAGGTTCAG+AGG	0.572504	3.4:+99603672	MS.gene063143:CDS
CAGCAAGAAGATCTCGAATG+AGG	0.587735	3.4:+99603755	MS.gene063143:CDS
CCAAGAATCGTTGAGTTGTG+AGG	0.604416	3.4:-99603858	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATTATTATTATTATCAAC+AGG	-	chr3.4:99603997-99604016	None:intergenic	10.0%
!	AATGAGGAAACAAAAACATT+TGG	+	chr3.4:99603771-99603790	MS.gene063143:CDS	25.0%
!	TCAAATAACCCATATTCAAA+GGG	-	chr3.4:99604038-99604057	None:intergenic	25.0%
!	TTCAAATAACCCATATTCAA+AGG	-	chr3.4:99604039-99604058	None:intergenic	25.0%
!	TTCAATAACCCTTTGAATAT+GGG	+	chr3.4:99604027-99604046	MS.gene063143:CDS	25.0%
	AACTTCATGAATGTTGCAAA+TGG	+	chr3.4:99603961-99603980	MS.gene063143:CDS	30.0%
	CTTCAATAACCCTTTGAATA+TGG	+	chr3.4:99604026-99604045	MS.gene063143:CDS	30.0%
	TTATTTGAACCAACCAATCA+TGG	+	chr3.4:99604050-99604069	MS.gene063143:CDS	30.0%
	TGTTCAACACTTCCAAATTC+AGG	+	chr3.4:99603664-99603683	MS.gene063143:CDS	35.0%
	ATATCTGCAGAAGCCATGAT+TGG	-	chr3.4:99604066-99604085	None:intergenic	40.0%
	GGAAGATTTGATGCTTCTGA+TGG	+	chr3.4:99603693-99603712	MS.gene063143:CDS	40.0%
	TCTTCAAGTGGAACATCTTC+AGG	+	chr3.4:99603640-99603659	MS.gene063143:CDS	40.0%
	TCTTCCTCTGAACCTGAATT+TGG	-	chr3.4:99603679-99603698	None:intergenic	40.0%
!	ACTTCCAAATTCAGGTTCAG+AGG	+	chr3.4:99603672-99603691	MS.gene063143:CDS	40.0%
!!	TTTGGATGATCTTGCTGTTC+AGG	+	chr3.4:99603789-99603808	MS.gene063143:CDS	40.0%
	CAGCAAGAAGATCTCGAATG+AGG	+	chr3.4:99603755-99603774	MS.gene063143:CDS	45.0%
	CCTCACAACTCAACGATTCT+TGG	+	chr3.4:99603858-99603877	MS.gene063143:CDS	45.0%
	CTGTTCAGGTGAGTCAACTA+AGG	+	chr3.4:99603803-99603822	MS.gene063143:CDS	45.0%
!	CCAAGAATCGTTGAGTTGTG+AGG	-	chr3.4:99603861-99603880	None:intergenic	45.0%
!!	GAGATCTTCTTGCTGATTCG+CGG	-	chr3.4:99603750-99603769	None:intergenic	45.0%
	CTGCAGAAGCCATGATTGGT+TGG	-	chr3.4:99604062-99604081	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	99603634	99604104	99603634	ID=MS.gene063143
chr3.4	mRNA	99603634	99604104	99603634	ID=MS.gene063143.t1;Parent=MS.gene063143
chr3.4	exon	99603634	99604104	99603634	ID=MS.gene063143.t1.exon1;Parent=MS.gene063143.t1
chr3.4	CDS	99603634	99604104	99603634	ID=cds.MS.gene063143.t1;Parent=MS.gene063143.t1

Gene Sequence

>MS.gene063143

ATGGCTTCTTCAAGTGGAACATCTTCAGGCTGTTCAACACTTCCAAATTCAGGTTCAGAGGAAGATTTGATGCTTCTGATGGATCAAAGAAAAAGAAAGAGAATGATATCAAACCGCGAATCAGCAAGAAGATCTCGAATGAGGAAACAAAAACATTTGGATGATCTTGCTGTTCAGGTGAGTCAACTAAGGAATGAGAATCAACAGATTCTAACTTCAGTTAACCTCACAACTCAACGATTCTTGGCTGTTGAATCTGAAAACTCTGTGCTTAGAGCTCAACTGAATGAACTCAACAGCAGATTTGAGTCTTTGAATGAGATCATCAACTTCATGAATGTTGCAAATGGTGTTTTTGAACCTGTTGATAATAATAATAATAATGACAATTACTTCAATAACCCTTTGAATATGGGTTATTTGAACCAACCAATCATGGCTTCTGCAGATATGAACATGATACATTATTGA

Protein sequence

>MS.gene063143.t1

MASSSGTSSGCSTLPNSGSEEDLMLLMDQRKRKRMISNRESARRSRMRKQKHLDDLAVQVSQLRNENQQILTSVNLTTQRFLAVESENSVLRAQLNELNSRFESLNEIINFMNVANGVFEPVDNNNNNDNYFNNPLNMGYLNQPIMASADMNMIHY