AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064124

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064124.t1 MTR_4g070080 86.022 93 13 0 1 93 1 93 2.40e-49 156
MS.gene064124.t1 MTR_4g070080 84.337 83 13 0 1 83 1 83 6.33e-49 156
MS.gene064124.t1 MTR_4g070140 80.723 83 16 0 2 84 4 86 1.46e-45 149
MS.gene064124.t1 MTR_4g070190 72.414 116 15 1 2 117 4 102 3.12e-42 139
MS.gene064124.t1 MTR_3g084040 53.165 79 36 1 7 85 37 114 6.17e-24 92.0
MS.gene064124.t1 MTR_3g075500 53.571 84 39 0 3 86 203 286 1.54e-23 94.7
MS.gene064124.t1 MTR_3g075500 41.975 81 47 0 7 87 110 190 6.21e-12 62.8
MS.gene064124.t1 MTR_4g109540 47.500 80 42 0 7 86 43 122 4.77e-23 92.8
MS.gene064124.t1 MTR_3g025880 46.835 79 42 0 7 85 45 123 1.19e-22 94.0
MS.gene064124.t1 MTR_7g013730 44.578 83 46 0 7 89 41 123 1.94e-22 90.9
MS.gene064124.t1 MTR_4g109540 48.052 77 40 0 10 86 54 130 1.81e-21 89.0
MS.gene064124.t1 MTR_3g080130 46.341 82 44 0 7 88 37 118 3.63e-21 84.7
MS.gene064124.t1 MTR_4g104470 51.852 81 39 0 6 86 216 296 5.51e-21 87.8
MS.gene064124.t1 MTR_4g104470 38.235 102 63 0 5 106 121 222 6.81e-16 73.9
MS.gene064124.t1 MTR_1g064230 55.000 80 36 0 7 86 197 276 7.49e-21 87.4
MS.gene064124.t1 MTR_3g014000 46.939 98 40 3 8 98 36 128 4.35e-20 82.0
MS.gene064124.t1 MTR_3g075500 55.882 68 30 0 3 70 203 270 6.81e-19 81.6
MS.gene064124.t1 MTR_3g075500 41.975 81 47 0 7 87 110 190 6.99e-12 62.8
MS.gene064124.t1 MTR_7g099940 49.367 79 40 0 7 85 36 114 8.01e-19 79.0
MS.gene064124.t1 MTR_4g035310 45.263 95 44 3 7 95 17 109 1.73e-18 80.9
MS.gene064124.t1 MTR_4g035310 45.263 95 44 3 7 95 17 109 2.04e-18 80.5
MS.gene064124.t1 MTR_7g105260 43.210 81 46 0 4 84 30 110 2.69e-18 77.4
MS.gene064124.t1 MTR_3g084040 51.562 64 30 1 7 70 37 99 6.47e-18 75.5
MS.gene064124.t1 MTR_6g477870 43.750 80 45 0 7 86 96 175 1.58e-16 73.9
MS.gene064124.t1 MTR_1g045510 43.902 82 46 0 2 83 200 281 1.89e-16 75.5
MS.gene064124.t1 MTR_3g055990 39.241 79 46 1 7 85 7 83 3.28e-16 75.9
MS.gene064124.t1 MTR_3g116130 40.708 113 63 3 7 116 30 141 3.43e-16 74.7
MS.gene064124.t1 MTR_3g055990 39.241 79 46 1 7 85 7 83 3.62e-16 75.9
MS.gene064124.t1 MTR_8g024520 32.812 128 70 2 7 119 26 152 7.33e-16 73.6
MS.gene064124.t1 MTR_8g024520 32.812 128 70 2 7 119 26 152 7.49e-16 73.6
MS.gene064124.t1 MTR_3g092490 34.862 109 69 2 7 115 27 133 1.06e-15 71.6
MS.gene064124.t1 MTR_3g092490 28.916 166 106 2 7 161 27 191 1.13e-15 72.8
MS.gene064124.t1 MTR_3g116130 40.708 113 63 3 7 116 30 141 1.16e-15 73.9
MS.gene064124.t1 MTR_4g081520 43.820 89 50 0 1 89 46 134 1.22e-15 70.5
MS.gene064124.t1 MTR_3g092490 38.298 94 57 1 7 100 27 119 1.24e-15 72.8
MS.gene064124.t1 MTR_5g023060 33.333 171 106 5 8 171 133 302 1.40e-15 73.2
MS.gene064124.t1 MTR_7g013740 39.640 111 54 1 7 117 41 138 3.34e-15 69.3
MS.gene064124.t1 MTR_2g096560 39.024 82 50 0 7 88 8 89 3.60e-15 67.8
MS.gene064124.t1 MTR_5g097580 40.506 79 45 2 7 85 7 83 1.69e-14 70.9
MS.gene064124.t1 MTR_5g097580 33.010 103 64 2 7 109 107 204 3.21e-11 61.6
MS.gene064124.t1 MTR_3g065190 29.577 142 98 2 8 147 114 255 1.72e-14 70.9
MS.gene064124.t1 MTR_5g097580 40.506 79 45 2 7 85 7 83 1.75e-14 70.9
MS.gene064124.t1 MTR_5g097580 33.010 103 64 2 7 109 107 204 3.85e-11 61.2
MS.gene064124.t1 MTR_1g073690 35.443 79 49 1 7 85 7 83 3.97e-14 69.7
MS.gene064124.t1 MTR_1g073690 34.483 87 56 1 7 93 113 198 2.69e-13 67.4
MS.gene064124.t1 MTR_2g026180 36.709 79 48 1 7 85 5 81 7.33e-14 68.9
MS.gene064124.t1 MTR_5g064120 42.105 76 43 1 7 82 17 91 1.56e-13 67.4
MS.gene064124.t1 MTR_1g092860 41.026 78 46 0 7 84 193 270 1.79e-13 67.4
MS.gene064124.t1 MTR_7g117495 47.458 59 31 0 7 65 19 77 4.94e-13 65.5
MS.gene064124.t1 MTR_7g117495 47.458 59 31 0 7 65 19 77 5.41e-13 65.5
MS.gene064124.t1 MTR_3g029580 40.541 74 44 0 7 80 33 106 1.38e-12 62.4
MS.gene064124.t1 MTR_4g063560 41.096 73 43 0 7 79 34 106 1.73e-12 61.2
MS.gene064124.t1 MTR_6g477870 42.188 64 37 0 7 70 96 159 3.48e-12 62.0
MS.gene064124.t1 MTR_4g085540 44.444 72 39 1 8 79 124 194 6.03e-12 63.5
MS.gene064124.t1 MTR_4g117350 35.165 91 58 1 7 97 72 161 1.11e-11 60.5
MS.gene064124.t1 MTR_4g036415 43.056 72 41 0 8 79 453 524 2.01e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064124.t1 AT4G39260 74.725 91 23 0 1 91 1 91 1.22e-46 148
MS.gene064124.t1 AT2G21660 79.012 81 17 0 3 83 5 85 7.43e-44 143
MS.gene064124.t1 AT2G21660 80.899 89 17 0 3 91 5 93 6.95e-43 141
MS.gene064124.t1 AT4G39260 72.289 83 23 0 1 83 1 83 1.80e-41 136
MS.gene064124.t1 AT4G39260 72.289 83 23 0 1 83 1 83 6.53e-41 136
MS.gene064124.t1 AT4G39260 70.588 68 20 0 1 68 1 68 2.41e-32 112
MS.gene064124.t1 AT3G26420 37.118 229 87 6 1 172 1 229 6.06e-28 105
MS.gene064124.t1 AT3G23830 56.627 83 36 0 7 89 36 118 7.66e-28 102
MS.gene064124.t1 AT3G23830 56.627 83 36 0 7 89 36 118 7.66e-28 102
MS.gene064124.t1 AT4G13850 54.217 83 38 0 7 89 36 118 3.43e-25 95.5
MS.gene064124.t1 AT4G13850 54.217 83 38 0 7 89 36 118 4.36e-25 95.5
MS.gene064124.t1 AT4G13850 54.217 83 38 0 7 89 36 118 4.96e-25 95.5
MS.gene064124.t1 AT4G13850 55.556 81 36 0 7 87 36 116 5.20e-25 94.4
MS.gene064124.t1 AT3G53460 51.807 83 40 0 7 89 258 340 9.04e-23 93.6
MS.gene064124.t1 AT3G53460 51.807 83 40 0 7 89 258 340 9.04e-23 93.6
MS.gene064124.t1 AT3G53460 51.807 83 40 0 7 89 258 340 9.04e-23 93.6
MS.gene064124.t1 AT2G37220 50.617 81 40 0 7 87 205 285 9.54e-23 92.4
MS.gene064124.t1 AT2G37220 43.421 76 43 0 5 80 90 165 3.29e-12 63.9
MS.gene064124.t1 AT3G53460 51.807 83 40 0 7 89 279 361 1.31e-22 93.2
MS.gene064124.t1 AT4G24770 51.220 82 40 0 6 87 244 325 5.60e-22 91.3
MS.gene064124.t1 AT4G24770 40.196 102 61 0 5 106 149 250 4.95e-16 74.7
MS.gene064124.t1 AT4G24770 51.220 82 40 0 6 87 244 325 5.60e-22 91.3
MS.gene064124.t1 AT4G24770 40.196 102 61 0 5 106 149 250 4.95e-16 74.7
MS.gene064124.t1 AT5G04280 45.161 93 51 0 7 99 8 100 2.66e-21 89.0
MS.gene064124.t1 AT5G06210 44.444 90 50 0 4 93 32 121 3.99e-20 82.4
MS.gene064124.t1 AT5G50250 50.000 80 40 0 6 85 207 286 6.02e-20 85.1
MS.gene064124.t1 AT5G50250 39.216 102 62 0 5 106 112 213 2.58e-15 72.4
MS.gene064124.t1 AT1G18630 50.000 78 39 0 7 84 37 114 8.29e-20 81.6
MS.gene064124.t1 AT2G21690 47.126 87 41 1 4 85 5 91 6.18e-19 78.6
MS.gene064124.t1 AT1G60650 42.683 82 47 0 4 85 10 91 1.46e-18 81.3
MS.gene064124.t1 AT1G60650 42.683 82 47 0 4 85 10 91 1.46e-18 81.3
MS.gene064124.t1 AT5G47320 49.351 77 39 0 7 83 32 108 4.48e-18 78.6
MS.gene064124.t1 AT2G46780 43.269 104 52 3 7 104 23 125 1.28e-17 79.3
MS.gene064124.t1 AT2G46780 43.269 104 52 3 7 104 23 125 1.28e-17 79.3
MS.gene064124.t1 AT5G61030 38.462 117 40 1 7 91 41 157 1.82e-17 78.6
MS.gene064124.t1 AT5G61030 38.462 117 40 1 7 91 41 157 1.82e-17 78.6
MS.gene064124.t1 AT2G37510 51.351 74 36 0 7 80 35 108 2.00e-17 75.1
MS.gene064124.t1 AT1G22910 44.681 94 46 2 7 95 14 106 2.59e-17 77.4
MS.gene064124.t1 AT1G22910 44.681 94 46 2 7 95 14 106 3.46e-17 76.6
MS.gene064124.t1 AT1G74230 53.571 112 51 1 7 118 35 145 1.52e-16 75.9
MS.gene064124.t1 AT3G07810 40.506 79 45 1 7 85 7 83 2.08e-16 76.6
MS.gene064124.t1 AT3G07810 40.506 79 45 1 7 85 7 83 2.14e-16 76.6
MS.gene064124.t1 AT1G22330 41.176 102 51 2 7 100 18 118 2.64e-16 75.1
MS.gene064124.t1 AT1G22910 44.681 94 46 2 7 95 14 106 2.84e-16 75.5
MS.gene064124.t1 AT2G33410 32.143 112 74 1 7 118 7 116 3.21e-16 75.9
MS.gene064124.t1 AT4G13860 45.205 73 40 0 7 79 4 76 5.43e-16 70.1
MS.gene064124.t1 AT1G20880 46.053 76 40 1 7 82 25 99 6.63e-16 73.9
MS.gene064124.t1 AT1G20880 46.053 76 40 1 7 82 25 99 6.63e-16 73.9
MS.gene064124.t1 AT1G20880 46.053 76 40 1 7 82 25 99 6.63e-16 73.9
MS.gene064124.t1 AT5G59865 46.835 79 42 0 9 87 29 107 1.03e-15 70.1
MS.gene064124.t1 AT1G33470 46.753 77 38 2 7 82 8 82 2.16e-15 72.0
MS.gene064124.t1 AT1G33470 46.753 77 38 2 7 82 8 82 2.49e-15 72.0
MS.gene064124.t1 AT1G33470 46.753 77 38 2 7 82 8 82 2.74e-15 71.6
MS.gene064124.t1 AT1G60000 45.122 82 45 0 2 83 173 254 2.91e-15 72.0
MS.gene064124.t1 AT1G76460 46.053 76 40 1 7 82 25 99 3.75e-15 72.0
MS.gene064124.t1 AT5G19960 37.037 108 66 2 8 115 9 114 4.19e-15 72.4
MS.gene064124.t1 AT3G08000 44.048 84 47 0 7 90 42 125 7.28e-15 68.6
MS.gene064124.t1 AT4G14300 37.975 79 47 1 7 85 7 83 8.77e-15 71.6
MS.gene064124.t1 AT4G14300 37.975 79 47 1 7 85 7 83 8.77e-15 71.6
MS.gene064124.t1 AT3G46020 39.286 84 50 1 1 83 1 84 1.07e-14 67.0
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.26e-14 71.2
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.26e-14 71.2
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.26e-14 71.2
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.26e-14 71.2
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.26e-14 71.2
MS.gene064124.t1 AT4G26650 37.975 79 47 1 7 85 13 89 1.53e-14 71.2
MS.gene064124.t1 AT4G26650 37.975 79 47 1 7 85 16 92 1.55e-14 71.2
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.65e-14 70.9
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.65e-14 70.9
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.65e-14 70.9
MS.gene064124.t1 AT5G55550 39.241 79 46 1 7 85 7 83 1.65e-14 70.9
MS.gene064124.t1 AT1G78260 43.421 76 42 1 7 82 18 92 3.09e-14 69.3
MS.gene064124.t1 AT1G78260 43.421 76 42 1 7 82 18 92 5.01e-14 68.6
MS.gene064124.t1 AT3G13224 34.568 81 53 0 7 87 110 190 1.01e-13 68.6
MS.gene064124.t1 AT3G13224 34.524 84 53 1 7 90 20 101 2.00e-12 64.7
MS.gene064124.t1 AT1G73530 36.250 80 51 0 7 86 78 157 2.04e-13 65.5
MS.gene064124.t1 AT2G23350 48.611 72 36 1 8 79 136 206 2.84e-13 67.4
MS.gene064124.t1 AT3G13224 34.568 81 53 0 7 87 101 181 3.07e-13 67.0
MS.gene064124.t1 AT3G13224 34.043 94 56 2 1 90 1 92 9.67e-13 65.5
MS.gene064124.t1 AT5G55550 40.278 72 41 1 7 78 7 76 3.16e-13 67.4
MS.gene064124.t1 AT5G55550 40.278 72 41 1 7 78 7 76 3.58e-13 67.0
MS.gene064124.t1 AT2G27330 29.670 91 64 0 9 99 24 114 4.19e-13 63.2
MS.gene064124.t1 AT5G53720 40.404 99 53 3 5 103 8 100 4.55e-13 62.8
MS.gene064124.t1 AT1G01080 41.463 82 45 2 1 81 207 286 5.92e-13 65.9
MS.gene064124.t1 AT1G01080 41.463 82 45 2 1 81 208 287 6.21e-13 65.9
MS.gene064124.t1 AT3G20930 37.500 80 50 0 7 86 283 362 9.53e-13 65.9
MS.gene064124.t1 AT5G51300 42.667 75 43 0 5 79 479 553 9.82e-13 65.9
MS.gene064124.t1 AT5G51300 42.667 75 43 0 5 79 479 553 9.82e-13 65.9
MS.gene064124.t1 AT5G51300 42.667 75 43 0 5 79 479 553 9.82e-13 65.9
MS.gene064124.t1 AT5G47620 35.443 79 49 1 7 85 7 83 1.05e-12 65.9
MS.gene064124.t1 AT5G47620 35.443 79 49 1 7 85 7 83 1.05e-12 65.9
MS.gene064124.t1 AT5G47620 35.443 79 49 1 7 85 7 83 1.05e-12 65.9
MS.gene064124.t1 AT3G54770 42.105 76 43 1 7 82 18 92 1.10e-12 64.7
MS.gene064124.t1 AT3G54770 42.105 76 43 1 7 82 18 92 1.26e-12 64.7
MS.gene064124.t1 AT1G17640 38.750 80 47 1 7 86 67 144 2.72e-12 64.3
MS.gene064124.t1 AT1G17640 32.990 97 64 1 7 103 158 253 3.43e-12 64.3
MS.gene064124.t1 AT1G17640 38.750 80 47 1 7 86 67 144 2.72e-12 64.3
MS.gene064124.t1 AT1G17640 32.990 97 64 1 7 103 158 253 3.43e-12 64.3
MS.gene064124.t1 AT1G17640 38.750 80 47 1 7 86 67 144 2.72e-12 64.3
MS.gene064124.t1 AT1G17640 32.990 97 64 1 7 103 158 253 3.43e-12 64.3
MS.gene064124.t1 AT5G54580 44.048 84 47 0 2 85 52 135 2.81e-12 62.0
MS.gene064124.t1 AT4G13860 46.667 60 32 0 21 80 1 60 6.07e-12 58.9
MS.gene064124.t1 AT3G52150 44.156 77 43 0 6 82 177 253 8.07e-12 62.4
MS.gene064124.t1 AT3G52150 44.156 77 43 0 6 82 177 253 8.07e-12 62.4
MS.gene064124.t1 AT2G37510 38.384 99 36 1 7 80 35 133 8.65e-12 60.8
MS.gene064124.t1 AT5G53680 49.254 67 27 2 7 66 14 80 9.83e-12 60.8
MS.gene064124.t1 AT5G47620 36.111 72 44 1 7 78 7 76 1.31e-11 62.8
MS.gene064124.t1 AT2G18510 32.759 116 76 2 8 121 114 229 1.56e-11 62.4
MS.gene064124.t1 AT3G14100 41.026 78 46 0 5 82 143 220 1.56e-11 62.4
MS.gene064124.t1 AT1G54080 41.026 78 46 0 5 82 147 224 3.83e-11 61.2
MS.gene064124.t1 AT2G22100 53.061 49 23 0 8 56 165 213 6.63e-11 60.5

Find 87 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGATATGGTGGAGGAGG+CGG 0.278119 3.2:+92145416 MS.gene064124:CDS
GGTGGATATGGTGGAGGTGG+CGG 0.285692 3.2:+92145359 MS.gene064124:CDS
GTGGATATGGTGGAGGTGGC+GGG 0.285761 3.2:+92145360 MS.gene064124:CDS
CGTGATGGTGGTGGTGGATA+TGG 0.293140 3.2:+92145467 MS.gene064124:CDS
ACTGGAAGATCAAGAGGATT+TGG 0.294131 3.2:+92145149 MS.gene064124:CDS
GTGGATATGGTGGAGGAGGC+GGG 0.301740 3.2:+92145417 MS.gene064124:CDS
CGCGGAGGTGGTGGTGGATA+TGG 0.305180 3.2:+92145311 MS.gene064124:CDS
CGTGGAGGAGGTGGTGGATA+TGG 0.306969 3.2:+92145404 MS.gene064124:CDS
CGTCGTGGAGGTGGTGGATA+TGG 0.325761 3.2:+92145347 MS.gene064124:CDS
AGTGGCGGAGGCGGTGGTGG+TGG 0.340361 3.2:+92145281 MS.gene064124:CDS
GTTATCACCGATCGTGAAAC+TGG 0.362798 3.2:+92145131 MS.gene064124:CDS
TGGATATGGTGGAGGTGGCG+GGG 0.366937 3.2:+92145361 MS.gene064124:CDS
GGATATGGTGGAGGTGGCGG+GGG 0.367929 3.2:+92145362 MS.gene064124:CDS
TGGATATGGTGGAGGAGGCG+GGG 0.372204 3.2:+92145418 MS.gene064124:CDS
ATCACCGCCTCTCGAGTATC+TGG 0.373006 3.2:-92145516 None:intergenic
GGCGGAGGCGGTGGTGGTGG+TGG 0.379371 3.2:+92145284 MS.gene064124:CDS
GGCAGTGGCGGAGGCGGTGG+TGG 0.383736 3.2:+92145278 MS.gene064124:CDS
GGATATGGTGGAGGAGGCGG+GGG 0.387784 3.2:+92145419 MS.gene064124:CDS
CGTGGCAGTGGCGGAGGCGG+TGG 0.400483 3.2:+92145275 MS.gene064124:CDS
GGATACCGCGGAGGTGGTGG+TGG 0.412147 3.2:+92145305 MS.gene064124:CDS
GGTGGATACCGCGGAGGTGG+TGG 0.418043 3.2:+92145302 MS.gene064124:CDS
GGTTACGGAGGTGGTGGTGG+CGG 0.440050 3.2:+92145440 MS.gene064124:CDS
GGTGGTGGATATGGTGGAGG+TGG 0.440960 3.2:+92145356 MS.gene064124:CDS
GGTGGCGAACGTCGTGGAGG+TGG 0.444469 3.2:+92145338 MS.gene064124:CDS
GGTGGTGACCGTCGTGGAGG+AGG 0.444501 3.2:+92145392 MS.gene064124:CDS
GACCGTCGTGGAGGAGGTGG+TGG 0.446949 3.2:+92145398 MS.gene064124:CDS
GGTGGTGGATATGGTGGAGG+AGG 0.448331 3.2:+92145413 MS.gene064124:CDS
GGTGGTGGATATGGCGGAGG+TGG 0.452527 3.2:+92145320 MS.gene064124:CDS
CAGAGTCGTGGCAGTGGCGG+AGG 0.453358 3.2:+92145269 MS.gene064124:CDS
GGTGGTGGATACCGCGGAGG+TGG 0.455995 3.2:+92145299 MS.gene064124:CDS
AGTCGTGGCAGTGGCGGAGG+CGG 0.456959 3.2:+92145272 MS.gene064124:CDS
GTGGCTCTGACAGTAGAAAC+TGG 0.459380 3.2:+92145543 MS.gene064124:CDS
GTTGAATACCGATGCTTTGT+TGG 0.464495 3.2:+92144747 MS.gene064124:CDS
GGTGACCGTCGTGGAGGAGG+TGG 0.465249 3.2:+92145395 MS.gene064124:CDS
CTTTGTTGGTGGACTTGCAT+GGG 0.465470 3.2:+92144761 MS.gene064124:CDS
GGCGAACGTCGTGGAGGTGG+TGG 0.469494 3.2:+92145341 MS.gene064124:CDS
GATGGTGGTGGTGGATATGG+TGG 0.469678 3.2:+92145470 MS.gene064124:CDS
GGTGGTGGATATGGTGGAGG+TGG 0.471808 3.2:+92145476 MS.gene064124:CDS
GGATATGGTGGAGGTGGCTA+CGG 0.473053 3.2:+92145482 MS.gene064124:CDS
GGAGGAGGTGGTGGATATGG+TGG 0.473329 3.2:+92145407 MS.gene064124:CDS
GGAGGTGGTGGATATGGTGG+AGG 0.473487 3.2:+92145410 MS.gene064124:CDS
CTCAAGAGCTTCGTTGTCAG+TGG 0.495714 3.2:-92144784 None:intergenic
GGTGGTGGTGGATATGGCGG+AGG 0.496613 3.2:+92145317 MS.gene064124:CDS
GGTGGTGGTGGATATGGTGG+AGG 0.497470 3.2:+92145473 MS.gene064124:CDS
GCGATGAAAGATGCGATTGA+AGG 0.503882 3.2:+92145197 MS.gene064124:CDS
ATGAACGGCCAAGATCTTGA+TGG 0.505893 3.2:+92145221 MS.gene064124:CDS
GGTGGAGGTGGCTACGGAGA+TGG 0.509409 3.2:+92145488 MS.gene064124:CDS
TGTCACATTCTCTACGGAGC+AGG 0.509697 3.2:+92145175 MS.gene064124:CDS
GGACGTCGTGATGGTGGTGG+TGG 0.511175 3.2:+92145461 MS.gene064124:CDS
CGTGGAGGTGGTGGATATGG+TGG 0.512534 3.2:+92145350 MS.gene064124:CDS
GGTGGTGGCGGACGTCGTGA+TGG 0.514583 3.2:+92145452 MS.gene064124:CDS
GCTTTGTTGGTGGACTTGCA+TGG 0.519425 3.2:+92144760 MS.gene064124:CDS
TGGATTTGTCACATTCTCTA+CGG 0.520808 3.2:+92145169 MS.gene064124:CDS
TGATCTTCCAGTTTCACGAT+CGG 0.521619 3.2:-92145138 None:intergenic
GAGGCTCAGAGTCGTGGCAG+TGG 0.522443 3.2:+92145263 MS.gene064124:CDS
TGTTACGTCCATCAAGATCT+TGG 0.527371 3.2:-92145229 None:intergenic
TCGAGAGGCGGTGATGGCGG+TGG 0.528930 3.2:+92145524 MS.gene064124:CDS
GGAGGTGGTGGATATGGTGG+AGG 0.534655 3.2:+92145353 MS.gene064124:CDS
GGCGGACGTCGTGATGGTGG+TGG 0.542567 3.2:+92145458 MS.gene064124:CDS
CGATGAAAGATGCGATTGAA+GGG 0.542649 3.2:+92145198 MS.gene064124:CDS
TGGTGAAATCGTCGAATCGA+AGG 0.542807 3.2:+92144824 MS.gene064124:CDS
GCTCAGAGTCGTGGCAGTGG+CGG 0.545233 3.2:+92145266 MS.gene064124:CDS
CGATTCGACGATTTCACCAA+AGG 0.547099 3.2:-92144820 None:intergenic
GGTGGCGGACGTCGTGATGG+TGG 0.554420 3.2:+92145455 MS.gene064124:CDS
AGATACTCGAGAGGCGGTGA+TGG 0.555850 3.2:+92145518 MS.gene064124:CDS
GGAGGTGGCTACGGAGATGG+TGG 0.556120 3.2:+92145491 MS.gene064124:CDS
CGTGAAACTGGAAGATCAAG+AGG 0.575579 3.2:+92145143 MS.gene064124:CDS
GAATACCGATGCTTTGTTGG+TGG 0.577401 3.2:+92144750 MS.gene064124:CDS
TACTCGAGAGGCGGTGATGG+CGG 0.579779 3.2:+92145521 MS.gene064124:CDS
ACGTAACATCACTGTGAATG+AGG 0.581570 3.2:+92145244 MS.gene064124:CDS
TCGGTATTCAACTTCCGCCA+TGG 0.595134 3.2:-92144736 None:intergenic
GGAGGTGGTGGTGGATATGG+CGG 0.597374 3.2:+92145314 MS.gene064124:CDS
TATGGAGGTGGTGACCGTCG+TGG 0.615014 3.2:+92145386 MS.gene064124:CDS
CTCTGACAGTAGAAACTGGA+GGG 0.619176 3.2:+92145547 MS.gene064124:CDS
GATGCGATTGAAGGGATGAA+CGG 0.619661 3.2:+92145206 MS.gene064124:CDS
GTGAATGAGGCTCAGAGTCG+TGG 0.619893 3.2:+92145257 MS.gene064124:CDS
GGTGGTGGTGGTGGATACCG+CGG 0.621729 3.2:+92145293 MS.gene064124:CDS
GGCGGAGGTGGCGAACGTCG+TGG 0.626375 3.2:+92145332 MS.gene064124:CDS
CAAGTCCACCAACAAAGCAT+CGG 0.636350 3.2:-92144755 None:intergenic
GGCTCCAGATACTCGAGAGG+CGG 0.636811 3.2:+92145512 MS.gene064124:CDS
GGAGGTGGCGAACGTCGTGG+AGG 0.643779 3.2:+92145335 MS.gene064124:CDS
GGAGGTGGTGACCGTCGTGG+AGG 0.653524 3.2:+92145389 MS.gene064124:CDS
GGTGGTGGTGGATACCGCGG+AGG 0.661432 3.2:+92145296 MS.gene064124:CDS
ATCCACCACCTCCTCCACGA+CGG 0.697052 3.2:-92145400 None:intergenic
CATATCCACCACCACCTCCG+CGG 0.713479 3.2:-92145310 None:intergenic
GCTCTGACAGTAGAAACTGG+AGG 0.714944 3.2:+92145546 MS.gene064124:CDS
GGTGGCTCCAGATACTCGAG+AGG 0.728248 3.2:+92145509 MS.gene064124:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTAATCACTTCAAAT+CGG + chr3.2:92144990-92145009 MS.gene064124:intron 15.0%
!!! ACGATTCTTTGTTTTTGTTT+AGG + chr3.2:92145109-92145128 MS.gene064124:intron 25.0%
TGTGTAAATTGAAGATCGAT+CGG + chr3.2:92145031-92145050 MS.gene064124:intron 30.0%
TGGATTTGTCACATTCTCTA+CGG + chr3.2:92145169-92145188 MS.gene064124:CDS 35.0%
ACAGAACAGAACAGAACAGA+AGG - chr3.2:92144908-92144927 None:intergenic 40.0%
ACGTAACATCACTGTGAATG+AGG + chr3.2:92145244-92145263 MS.gene064124:CDS 40.0%
ACTGGAAGATCAAGAGGATT+TGG + chr3.2:92145149-92145168 MS.gene064124:CDS 40.0%
ATCGGAAATCTGACACGATT+CGG + chr3.2:92144863-92144882 MS.gene064124:intron 40.0%
CGATGAAAGATGCGATTGAA+GGG + chr3.2:92145198-92145217 MS.gene064124:CDS 40.0%
GTTGAATACCGATGCTTTGT+TGG + chr3.2:92144747-92144766 MS.gene064124:CDS 40.0%
TGATCTTCCAGTTTCACGAT+CGG - chr3.2:92145141-92145160 None:intergenic 40.0%
TGTTACGTCCATCAAGATCT+TGG - chr3.2:92145232-92145251 None:intergenic 40.0%
TTTCCGATCTCAGCAACAAA+CGG - chr3.2:92144851-92144870 None:intergenic 40.0%
! AATCTGACACGATTCGGATT+TGG + chr3.2:92144869-92144888 MS.gene064124:intron 40.0%
!!! GAGAAAGCGTTTTCTTCCTT+TGG + chr3.2:92144804-92144823 MS.gene064124:CDS 40.0%
ATGAACGGCCAAGATCTTGA+TGG + chr3.2:92145221-92145240 MS.gene064124:CDS 45.0%
CAAGTCCACCAACAAAGCAT+CGG - chr3.2:92144758-92144777 None:intergenic 45.0%
CGATTCGACGATTTCACCAA+AGG - chr3.2:92144823-92144842 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG + chr3.2:92145143-92145162 MS.gene064124:CDS 45.0%
CTCTGACAGTAGAAACTGGA+GGG + chr3.2:92145547-92145566 MS.gene064124:CDS 45.0%
GATGCGATTGAAGGGATGAA+CGG + chr3.2:92145206-92145225 MS.gene064124:CDS 45.0%
GCGATGAAAGATGCGATTGA+AGG + chr3.2:92145197-92145216 MS.gene064124:CDS 45.0%
GTTATCACCGATCGTGAAAC+TGG + chr3.2:92145131-92145150 MS.gene064124:CDS 45.0%
TGGTGAAATCGTCGAATCGA+AGG + chr3.2:92144824-92144843 MS.gene064124:CDS 45.0%
! GAATACCGATGCTTTGTTGG+TGG + chr3.2:92144750-92144769 MS.gene064124:CDS 45.0%
!! CTTTGTTGGTGGACTTGCAT+GGG + chr3.2:92144761-92144780 MS.gene064124:CDS 45.0%
CTCAAGAGCTTCGTTGTCAG+TGG - chr3.2:92144787-92144806 None:intergenic 50.0%
GCTCTGACAGTAGAAACTGG+AGG + chr3.2:92145546-92145565 MS.gene064124:CDS 50.0%
TGTCACATTCTCTACGGAGC+AGG + chr3.2:92145175-92145194 MS.gene064124:CDS 50.0%
! GGTCCGTTTGTTGCTGAGAT+CGG + chr3.2:92144845-92144864 MS.gene064124:intron 50.0%
!! GCTTTGTTGGTGGACTTGCA+TGG + chr3.2:92144760-92144779 MS.gene064124:CDS 50.0%
!! GTGGCTCTGACAGTAGAAAC+TGG + chr3.2:92145543-92145562 MS.gene064124:CDS 50.0%
AGATACTCGAGAGGCGGTGA+TGG + chr3.2:92145518-92145537 MS.gene064124:CDS 55.0%
ATCACCGCCTCTCGAGTATC+TGG - chr3.2:92145519-92145538 None:intergenic 55.0%
GTGAATGAGGCTCAGAGTCG+TGG + chr3.2:92145257-92145276 MS.gene064124:CDS 55.0%
! GGATATGGTGGAGGTGGCTA+CGG + chr3.2:92145482-92145501 MS.gene064124:CDS 55.0%
!! CGTGATGGTGGTGGTGGATA+TGG + chr3.2:92145467-92145486 MS.gene064124:CDS 55.0%
!! GATGGTGGTGGTGGATATGG+TGG + chr3.2:92145470-92145489 MS.gene064124:CDS 55.0%
ATCCACCACCTCCTCCACGA+CGG - chr3.2:92145403-92145422 None:intergenic 60.0%
CATATCCACCACCACCTCCG+CGG - chr3.2:92145313-92145332 None:intergenic 60.0%
GGCTCCAGATACTCGAGAGG+CGG + chr3.2:92145512-92145531 MS.gene064124:CDS 60.0%
GGTGGCTCCAGATACTCGAG+AGG + chr3.2:92145509-92145528 MS.gene064124:CDS 60.0%
TATGGAGGTGGTGACCGTCG+TGG + chr3.2:92145386-92145405 MS.gene064124:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+CGG + chr3.2:92145416-92145435 MS.gene064124:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+CGG + chr3.2:92145359-92145378 MS.gene064124:CDS 60.0%
! GTGGATATGGTGGAGGAGGC+GGG + chr3.2:92145417-92145436 MS.gene064124:CDS 60.0%
! GTGGATATGGTGGAGGTGGC+GGG + chr3.2:92145360-92145379 MS.gene064124:CDS 60.0%
! TACTCGAGAGGCGGTGATGG+CGG + chr3.2:92145521-92145540 MS.gene064124:CDS 60.0%
! TGGATATGGTGGAGGAGGCG+GGG + chr3.2:92145418-92145437 MS.gene064124:CDS 60.0%
! TGGATATGGTGGAGGTGGCG+GGG + chr3.2:92145361-92145380 MS.gene064124:CDS 60.0%
!! CGTCGTGGAGGTGGTGGATA+TGG + chr3.2:92145347-92145366 MS.gene064124:CDS 60.0%
!! CGTGGAGGAGGTGGTGGATA+TGG + chr3.2:92145404-92145423 MS.gene064124:CDS 60.0%
!! CGTGGAGGTGGTGGATATGG+TGG + chr3.2:92145350-92145369 MS.gene064124:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + chr3.2:92145407-92145426 MS.gene064124:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG + chr3.2:92145353-92145372 MS.gene064124:CDS 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG + chr3.2:92145314-92145333 MS.gene064124:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + chr3.2:92145356-92145375 MS.gene064124:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + chr3.2:92145356-92145375 MS.gene064124:CDS 60.0%
!! GGTGGTGGTGGATATGGTGG+AGG + chr3.2:92145473-92145492 MS.gene064124:CDS 60.0%
GAGGCTCAGAGTCGTGGCAG+TGG + chr3.2:92145263-92145282 MS.gene064124:CDS 65.0%
GCTCAGAGTCGTGGCAGTGG+CGG + chr3.2:92145266-92145285 MS.gene064124:CDS 65.0%
GGAGGTGGCTACGGAGATGG+TGG + chr3.2:92145491-92145510 MS.gene064124:CDS 65.0%
GGTGGAGGTGGCTACGGAGA+TGG + chr3.2:92145488-92145507 MS.gene064124:CDS 65.0%
! GGATATGGTGGAGGAGGCGG+GGG + chr3.2:92145419-92145438 MS.gene064124:CDS 65.0%
! GGATATGGTGGAGGTGGCGG+GGG + chr3.2:92145362-92145381 MS.gene064124:CDS 65.0%
!! CGCGGAGGTGGTGGTGGATA+TGG + chr3.2:92145311-92145330 MS.gene064124:CDS 65.0%
!! GGACGTCGTGATGGTGGTGG+TGG + chr3.2:92145461-92145480 MS.gene064124:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG + chr3.2:92145320-92145339 MS.gene064124:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG + chr3.2:92145317-92145336 MS.gene064124:CDS 65.0%
!! GGTGGTGGTGGTGGATACCG+CGG + chr3.2:92145293-92145312 MS.gene064124:CDS 65.0%
!! GGTTACGGAGGTGGTGGTGG+CGG + chr3.2:92145440-92145459 MS.gene064124:CDS 65.0%
CAGAGTCGTGGCAGTGGCGG+AGG + chr3.2:92145269-92145288 MS.gene064124:CDS 70.0%
GACCGTCGTGGAGGAGGTGG+TGG + chr3.2:92145398-92145417 MS.gene064124:CDS 70.0%
GGAGGTGGCGAACGTCGTGG+AGG + chr3.2:92145335-92145354 MS.gene064124:CDS 70.0%
GGAGGTGGTGACCGTCGTGG+AGG + chr3.2:92145389-92145408 MS.gene064124:CDS 70.0%
GGCGAACGTCGTGGAGGTGG+TGG + chr3.2:92145341-92145360 MS.gene064124:CDS 70.0%
GGTGACCGTCGTGGAGGAGG+TGG + chr3.2:92145395-92145414 MS.gene064124:CDS 70.0%
GGTGGAGGAGGCGGGGGTTA+CGG + chr3.2:92145425-92145444 MS.gene064124:CDS 70.0%
GGTGGATACCGCGGAGGTGG+TGG + chr3.2:92145302-92145321 MS.gene064124:CDS 70.0%
GGTGGCGAACGTCGTGGAGG+TGG + chr3.2:92145338-92145357 MS.gene064124:CDS 70.0%
GGTGGTGACCGTCGTGGAGG+AGG + chr3.2:92145392-92145411 MS.gene064124:CDS 70.0%
! AGTCGTGGCAGTGGCGGAGG+CGG + chr3.2:92145272-92145291 MS.gene064124:CDS 70.0%
! GGATACCGCGGAGGTGGTGG+TGG + chr3.2:92145305-92145324 MS.gene064124:CDS 70.0%
! GGGGGTTACGGAGGTGGTGG+TGG + chr3.2:92145437-92145456 MS.gene064124:CDS 70.0%
!! GGAGGTGGCGGGGGTTATGG+AGG + chr3.2:92145371-92145390 MS.gene064124:CDS 70.0%
!! GGCGGACGTCGTGATGGTGG+TGG + chr3.2:92145458-92145477 MS.gene064124:CDS 70.0%
!! GGTGGAGGTGGCGGGGGTTA+TGG + chr3.2:92145368-92145387 MS.gene064124:CDS 70.0%
!! GGTGGCGGACGTCGTGATGG+TGG + chr3.2:92145455-92145474 MS.gene064124:CDS 70.0%
!! GGTGGCGGGGGTTATGGAGG+TGG + chr3.2:92145374-92145393 MS.gene064124:CDS 70.0%
!! GGTGGTGGATACCGCGGAGG+TGG + chr3.2:92145299-92145318 MS.gene064124:CDS 70.0%
!! GGTGGTGGCGGACGTCGTGA+TGG + chr3.2:92145452-92145471 MS.gene064124:CDS 70.0%
!! GGTGGTGGTGGATACCGCGG+AGG + chr3.2:92145296-92145315 MS.gene064124:CDS 70.0%
!! TCGAGAGGCGGTGATGGCGG+TGG + chr3.2:92145524-92145543 MS.gene064124:CDS 70.0%
GGAGGAGGCGGGGGTTACGG+AGG + chr3.2:92145428-92145447 MS.gene064124:CDS 75.0%
GGAGGCGGGGGTTACGGAGG+TGG + chr3.2:92145431-92145450 MS.gene064124:CDS 75.0%
GGCGGAGGTGGCGAACGTCG+TGG + chr3.2:92145332-92145351 MS.gene064124:CDS 75.0%
GGCGGGGGTTACGGAGGTGG+TGG + chr3.2:92145434-92145453 MS.gene064124:CDS 75.0%
! AGTGGCGGAGGCGGTGGTGG+TGG + chr3.2:92145281-92145300 MS.gene064124:CDS 75.0%
!! CGTGGCAGTGGCGGAGGCGG+TGG + chr3.2:92145275-92145294 MS.gene064124:CDS 80.0%
!! GGCAGTGGCGGAGGCGGTGG+TGG + chr3.2:92145278-92145297 MS.gene064124:CDS 80.0%
!!! GGCGGAGGCGGTGGTGGTGG+TGG + chr3.2:92145284-92145303 MS.gene064124:CDS 80.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 92144738 92145574 92144738 ID=MS.gene064124
chr3.2 mRNA 92144738 92145574 92144738 ID=MS.gene064124.t1;Parent=MS.gene064124
chr3.2 exon 92144738 92144845 92144738 ID=MS.gene064124.t1.exon1;Parent=MS.gene064124.t1
chr3.2 CDS 92144738 92144845 92144738 ID=cds.MS.gene064124.t1;Parent=MS.gene064124.t1
chr3.2 exon 92145131 92145574 92145131 ID=MS.gene064124.t1.exon2;Parent=MS.gene064124.t1
chr3.2 CDS 92145131 92145574 92145131 ID=cds.MS.gene064124.t1;Parent=MS.gene064124.t1
Gene Sequence

>MS.gene064124

ATGGCGGAAGTTGAATACCGATGCTTTGTTGGTGGACTTGCATGGGCCACTGACAACGAAGCTCTTGAGAAAGCGTTTTCTTCCTTTGGTGAAATCGTCGAATCGAAGGTCCGTTTGTTGCTGAGATCGGAAATCTGACACGATTCGGATTTGGATTTTCGTGATCTCCTTCTGTTCTGTTCTGTTCTGTGTTGTTACTCTCTGATTACTTCTTCATCTTTGATACTCATCTGTTACTCTATAATGATTATCTTTTATTAATCACTTCAAATCGGTACGTTCATCTTCAATTTTGTGTAAATTGAAGATCGATCGGTTACTATCATTTTTTTTTATTTTTATAATTTATGCGAAATTGAGCTAGATCTGATACGATTCTTTGTTTTTGTTTAGGTTATCACCGATCGTGAAACTGGAAGATCAAGAGGATTTGGATTTGTCACATTCTCTACGGAGCAGGCGATGAAAGATGCGATTGAAGGGATGAACGGCCAAGATCTTGATGGACGTAACATCACTGTGAATGAGGCTCAGAGTCGTGGCAGTGGCGGAGGCGGTGGTGGTGGTGGATACCGCGGAGGTGGTGGTGGATATGGCGGAGGTGGCGAACGTCGTGGAGGTGGTGGATATGGTGGAGGTGGCGGGGGTTATGGAGGTGGTGACCGTCGTGGAGGAGGTGGTGGATATGGTGGAGGAGGCGGGGGTTACGGAGGTGGTGGTGGCGGACGTCGTGATGGTGGTGGTGGATATGGTGGAGGTGGCTACGGAGATGGTGGCTCCAGATACTCGAGAGGCGGTGATGGCGGTGGCTCTGACAGTAGAAACTGGAGGGAATAA
Protein sequence

>MS.gene064124.t1

MAEVEYRCFVGGLAWATDNEALEKAFSSFGEIVESKVITDRETGRSRGFGFVTFSTEQAMKDAIEGMNGQDLDGRNITVNEAQSRGSGGGGGGGGYRGGGGGYGGGGERRGGGGYGGGGGGYGGGDRRGGGGGYGGGGGGYGGGGGGRRDGGGGYGGGGYGDGGSRYSRGGDGGGSDSRNWRE