Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064920.t1 | XP_013452267.1 | 95.9 | 49 | 2 | 0 | 4 | 52 | 123 | 171 | 7.90E-18 | 99 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064920.t1 | O49668 | 54.0 | 50 | 23 | 0 | 3 | 52 | 151 | 200 | 8.0e-11 | 67.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064920.t1 | A0A072UKD7 | 95.9 | 49 | 2 | 0 | 4 | 52 | 123 | 171 | 5.7e-18 | 99.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene064920 | 0.832148 | 1.12E-55 | -1.69E-46 |
| MS.gene050406 | MS.gene064920 | 0.830394 | 3.01E-55 | -1.69E-46 |
| MS.gene052011 | MS.gene064920 | -0.810052 | 1.40E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene064920 | 0.814525 | 1.47E-51 | -1.69E-46 |
| MS.gene052253 | MS.gene064920 | 0.838809 | 2.31E-57 | -1.69E-46 |
| MS.gene052255 | MS.gene064920 | 0.806997 | 6.27E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene064920 | 0.818073 | 2.37E-52 | -1.69E-46 |
| MS.gene054349 | MS.gene064920 | 0.809443 | 1.89E-50 | -1.69E-46 |
| MS.gene054918 | MS.gene064920 | 0.825556 | 4.40E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene064920 | 0.836652 | 8.25E-57 | -1.69E-46 |
| MS.gene056899 | MS.gene064920 | -0.814114 | 1.82E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene064920 | 0.813015 | 3.17E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene064920 | 0.811026 | 8.60E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene064920 | 0.820035 | 8.48E-53 | -1.69E-46 |
| MS.gene058142 | MS.gene064920 | 0.809556 | 1.78E-50 | -1.69E-46 |
| MS.gene05886 | MS.gene064920 | 0.812589 | 3.93E-51 | -1.69E-46 |
| MS.gene059011 | MS.gene064920 | 0.802722 | 4.90E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene064920 | 0.802636 | 5.11E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene064920 | 0.801828 | 7.49E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene064920 | 0.810695 | 1.01E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene064920 | 0.832257 | 1.05E-55 | -1.69E-46 |
| MS.gene061114 | MS.gene064920 | -0.80623 | 9.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064920.t1 | MTR_6g051780 | 95.918 | 49 | 2 | 0 | 4 | 52 | 123 | 171 | 1.33e-29 | 102 |
| MS.gene064920.t1 | MTR_5g027010 | 47.619 | 63 | 27 | 1 | 3 | 59 | 139 | 201 | 9.05e-16 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064920.t1 | AT5G52780 | 56.000 | 50 | 22 | 0 | 4 | 53 | 112 | 161 | 1.03e-15 | 66.6 |
| MS.gene064920.t1 | AT4G19100 | 52.941 | 51 | 24 | 0 | 2 | 52 | 87 | 137 | 2.03e-15 | 65.9 |
| MS.gene064920.t1 | AT4G19100 | 52.941 | 51 | 24 | 0 | 2 | 52 | 150 | 200 | 2.91e-15 | 66.6 |
Find 0 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATACAAGAGTTGTCACAAA+TGG | + | 37806:6525-6544 | MS.gene06492:intergenic | 30.0% | |
| TTGAAGAAGTTGAGAAGAAT+TGG | - | 37806:6628-6647 | MS.gene064920:CDS | 30.0% | |
| !!! | GTGACAACTCTTGTATTTTT+TGG | - | 37806:6528-6547 | MS.gene064920:CDS | 30.0% |
| CAAATATTGATGCTCCTTTG+TGG | - | 37806:6496-6515 | MS.gene064920:CDS | 35.0% | |
| !!! | CTTGTATTTTTTGGAGCTTC+TGG | - | 37806:6537-6556 | MS.gene064920:CDS | 35.0% |
| AGAGAGAGAAGGTTCTTTGT+TGG | - | 37806:6602-6621 | MS.gene064920:CDS | 40.0% | |
| GAGAAGAATTGGTTGAGATG+TGG | - | 37806:6639-6658 | MS.gene064920:CDS | 40.0% | |
| GAGAGAGAAGGTTCTTTGTT+GGG | - | 37806:6603-6622 | MS.gene064920:CDS | 40.0% | |
| TCTGGACTTGGAATTGCATA+TGG | - | 37806:6555-6574 | MS.gene064920:CDS | 40.0% | |
| ! | TGGTGTGCTTTCTTCTAGTT+TGG | - | 37806:6575-6594 | MS.gene064920:CDS | 40.0% |
| !!! | TTTTTTGGAGCTTCTGGACT+TGG | - | 37806:6543-6562 | MS.gene064920:CDS | 40.0% |
| AGTTTGGATGCAGAGAGAGA+AGG | - | 37806:6591-6610 | MS.gene064920:CDS | 45.0% | |
| GTCACAAATGGCATCCACAA+AGG | + | 37806:6513-6532 | MS.gene064920:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37806 | gene | 6495 | 6673 | 6495 | ID=MS.gene064920 |
| 37806 | mRNA | 6495 | 6673 | 6495 | ID=MS.gene064920.t1;Parent=MS.gene064920 |
| 37806 | exon | 6495 | 6673 | 6495 | ID=MS.gene064920.t1.exon1;Parent=MS.gene064920.t1 |
| 37806 | CDS | 6495 | 6673 | 6495 | ID=cds.MS.gene064920.t1;Parent=MS.gene064920.t1 |
| Gene Sequence |
| Protein sequence |
