AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052255


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052255.t1 MTR_3g031360 89.205 176 13 4 1 171 1 175 8.44e-105 303
MS.gene052255.t1 MTR_1g022030 34.932 146 88 6 30 171 235 377 2.66e-15 72.8
MS.gene052255.t1 MTR_5g019790 40.777 103 56 4 71 171 219 318 2.02e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052255.t1 AT2G30170 70.874 103 30 0 69 171 61 163 7.93e-48 155
MS.gene052255.t1 AT2G30170 70.874 103 30 0 69 171 26 128 1.45e-47 154
MS.gene052255.t1 AT2G30170 70.874 103 30 0 69 171 61 163 4.70e-47 154
MS.gene052255.t1 AT2G30170 70.874 103 30 0 69 171 61 163 6.56e-47 155
MS.gene052255.t1 AT2G30170 68.605 86 27 0 86 171 1 86 1.91e-36 125
MS.gene052255.t1 AT2G30170 68.605 86 27 0 86 171 1 86 3.01e-36 125
MS.gene052255.t1 AT4G16580 42.478 113 60 5 60 170 223 332 6.96e-18 80.1
MS.gene052255.t1 AT5G66720 33.529 170 100 7 4 170 126 285 7.75e-18 80.1
MS.gene052255.t1 AT5G66720 33.140 172 95 7 4 170 126 282 1.42e-17 79.3
MS.gene052255.t1 AT4G33500 40.196 102 57 3 71 171 497 595 3.89e-16 75.5

Find 0 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTACTATTACCTTTTT+TGG + 20889:446-465 MS.gene052255:intergenic 15.0%
!!! TAATGCTTAATACTTTTTTT+AGG - 20889:337-356 MS.gene052255:intron 15.0%
!!! TTTTCAGTTATAAATTTAGA+TGG - 20889:913-932 MS.gene052255:intron 15.0%
!! TTAAATTGTTTATCCAAACA+AGG - 20889:835-854 MS.gene052255:intron 20.0%
!!! AAGTTTATCCTCTTTTATTT+AGG - 20889:1381-1400 MS.gene052255:intron 20.0%
!!! TTATAATTTTGTACAAGATG+AGG - 20889:1067-1086 MS.gene052255:intron 20.0%
!!! TTTTATTTAGGTTGCTAATT+TGG - 20889:1393-1412 MS.gene052255:intron 20.0%
! AGTTTACTAACCTAATTACA+GGG + 20889:296-315 MS.gene052255:intergenic 25.0%
! ATATAAAAACTTACACCTTC+TGG + 20889:772-791 MS.gene052255:intergenic 25.0%
! ATTAGCAACCTAAATAAAAG+AGG + 20889:1392-1411 MS.gene052255:intergenic 25.0%
! CAACTAATAAATGATGTATC+TGG + 20889:715-734 MS.gene052255:intergenic 25.0%
! CTATTGATGAAAATGCTAAA+AGG - 20889:229-248 MS.gene052255:intron 25.0%
! CTTACTCACTAATTCATTAT+TGG - 20889:630-649 MS.gene052255:intron 25.0%
! TTCAAAAGAATGAATGTAAC+TGG + 20889:1158-1177 MS.gene052255:intergenic 25.0%
! TTCAATATTAAGAGCTGTAA+TGG - 20889:158-177 MS.gene052255:CDS 25.0%
! TTTAGGTCTATGTTAGAATT+GGG - 20889:354-373 MS.gene052255:intron 25.0%
!!! TTTTAGGTCTATGTTAGAAT+TGG - 20889:353-372 MS.gene052255:intron 25.0%
AAATTGTTGAGGCTATATTG+TGG + 20889:1468-1487 MS.gene052254:intergenic 30.0%
AATCTAAAGATTGCCAATGT+TGG - 20889:1537-1556 MS.gene052255:CDS 30.0%
ATAATCAGTATCTGCGTTTA+GGG + 20889:1103-1122 MS.gene052255:intergenic 30.0%
ATGATCCTCAGATTCTTATA+AGG - 20889:1315-1334 MS.gene052255:CDS 30.0%
ATGGTTATATGCATGTTGTT+TGG - 20889:1233-1252 MS.gene052255:intron 30.0%
GAAGATATGTTATGAATGTG+TGG + 20889:210-229 MS.gene052255:intergenic 30.0%
GAGCTGTAATTCAATAAGAA+TGG - 20889:1214-1233 MS.gene052255:intron 30.0%
GAGTTTACTAACCTAATTAC+AGG + 20889:297-316 MS.gene052255:intergenic 30.0%
TTACTTTATAGGTCATCAGA+GGG - 20889:385-404 MS.gene052255:CDS 30.0%
TTCTTTGTGAGCAACTATAA+TGG - 20889:566-585 MS.gene052255:intron 30.0%
TTGATGAAAATGCTAAAAGG+AGG - 20889:232-251 MS.gene052255:intron 30.0%
TTTACTTTATAGGTCATCAG+AGG - 20889:384-403 MS.gene052255:CDS 30.0%
! AATGATAGTAATGCCTTGTT+TGG + 20889:851-870 MS.gene052255:intergenic 30.0%
! TAATTTTGTACAAGATGAGG+AGG - 20889:1070-1089 MS.gene052255:intron 30.0%
! TCTATGTTAGAATTGGGTTT+TGG - 20889:360-379 MS.gene052255:CDS 30.0%
!! AACAAAGCAAGATTTTAGTC+AGG + 20889:894-913 MS.gene052255:intergenic 30.0%
!! ACAAAGCAAGATTTTAGTCA+GGG + 20889:893-912 MS.gene052255:intergenic 30.0%
!! CATGTTGATTCGTTGAATAT+TGG - 20889:1273-1292 MS.gene052255:intron 30.0%
!! TGAATATTGGTTGATTTTGC+AGG - 20889:1286-1305 MS.gene052255:intron 30.0%
!!! ACTATTACCTTTTTTGGATG+TGG + 20889:440-459 MS.gene052255:intergenic 30.0%
!!! GGGTTTTGGTTTTACTTTAT+AGG - 20889:374-393 MS.gene052255:CDS 30.0%
AATTCACGTGGGAATAATGA+AGG + 20889:1035-1054 MS.gene052255:intergenic 35.0%
ATTATTCCCACGTGAATTGA+TGG - 20889:1037-1056 MS.gene052255:intron 35.0%
ATTGCTATGCTGGAAAAGAA+TGG - 20889:1513-1532 MS.gene052255:CDS 35.0%
CAGAACTTAACCCTGTAATT+AGG - 20889:283-302 MS.gene052255:intron 35.0%
CATAATCAGTATCTGCGTTT+AGG + 20889:1104-1123 MS.gene052255:intergenic 35.0%
CCTTATTCCACATCCAAAAA+AGG - 20889:430-449 MS.gene052255:intron 35.0%
GCTTTCCTTATAAGAATCTG+AGG + 20889:1323-1342 MS.gene052255:intergenic 35.0%
TAATCCACAATCACCAACAT+TGG + 20889:1553-1572 MS.gene052255:intergenic 35.0%
TCACAGACAAGAAATTGTTG+AGG + 20889:1479-1498 MS.gene052255:intergenic 35.0%
TGCTTATAATAGTGACCAGA+AGG - 20889:754-773 MS.gene052255:intron 35.0%
TGTTTCTTGAAGGTTGAGAA+AGG - 20889:530-549 MS.gene052255:intron 35.0%
TTGCTATGCTGGAAAAGAAT+GGG - 20889:1514-1533 MS.gene052255:CDS 35.0%
TTGTGTTATGCTTCACAGTT+GGG - 20889:992-1011 MS.gene052255:intron 35.0%
TTGTTAGAGACTTAGAGTTG+TGG - 20889:507-526 MS.gene052255:intron 35.0%
TTTGTGAGCAACTATAATGG+TGG - 20889:569-588 MS.gene052255:intron 35.0%
! AGAGGGTTTCATTTTCTGTT+GGG - 20889:402-421 MS.gene052255:CDS 35.0%
!!! CCTTTTTTGGATGTGGAATA+AGG + 20889:433-452 MS.gene052255:intergenic 35.0%
AGAGTTGTGGTGTTTCTTGA+AGG - 20889:520-539 MS.gene052255:intron 40.0%
CATTAGCCATCAATTCACGT+GGG + 20889:1046-1065 MS.gene052255:intergenic 40.0%
GTTGTGTTATGCTTCACAGT+TGG - 20889:991-1010 MS.gene052255:intron 40.0%
TCTTGAAGGTTGAGAAAGGT+GGG - 20889:534-553 MS.gene052255:intron 40.0%
TTCTTGAAGGTTGAGAAAGG+TGG - 20889:533-552 MS.gene052255:intron 40.0%
! CAGAGGGTTTCATTTTCTGT+TGG - 20889:401-420 MS.gene052255:CDS 40.0%
!! ATTGCCAATGTTGGTGATTG+TGG - 20889:1546-1565 MS.gene052255:CDS 40.0%
CATGCTGCTACATTCTCTAC+AGG - 20889:1344-1363 MS.gene052255:intron 45.0%
CTTGAAGGTTGAGAAAGGTG+GGG - 20889:535-554 MS.gene052255:intron 45.0%
GCATTAGCCATCAATTCACG+TGG + 20889:1047-1066 MS.gene052255:intergenic 45.0%
! CAGTATCTGCGTTTAGGGTA+CGG + 20889:1098-1117 MS.gene052255:intergenic 45.0%
! GGAGTTATTGCTGTTGCTGA+TGG - 20889:590-609 MS.gene052255:intron 45.0%
! GTGAAGCATCATTGCTATGC+TGG - 20889:1503-1522 MS.gene052255:CDS 45.0%
! CTGTTGCTGATGGTGTCTCT+GGG - 20889:600-619 MS.gene052255:intron 50.0%
GCTTCACAGTTGGGCTGAAG+AGG - 20889:1001-1020 MS.gene052255:intron 55.0%
! GCTGTTGCTGATGGTGTCTC+TGG - 20889:599-618 MS.gene052255:intron 55.0%


Chromosome Type Strat End Strand Name
20889 gene 150 1588 150 ID=MS.gene052255
20889 mRNA 150 1588 150 ID=MS.gene052255.t1;Parent=MS.gene052255
20889 exon 1434 1588 1434 ID=MS.gene052255.t1.exon1;Parent=MS.gene052255.t1
20889 CDS 1434 1588 1434 ID=cds.MS.gene052255.t1;Parent=MS.gene052255.t1
20889 exon 1287 1342 1287 ID=MS.gene052255.t1.exon2;Parent=MS.gene052255.t1
20889 CDS 1287 1342 1287 ID=cds.MS.gene052255.t1;Parent=MS.gene052255.t1
20889 exon 1117 1186 1117 ID=MS.gene052255.t1.exon3;Parent=MS.gene052255.t1
20889 CDS 1117 1186 1117 ID=cds.MS.gene052255.t1;Parent=MS.gene052255.t1
20889 exon 647 728 647 ID=MS.gene052255.t1.exon4;Parent=MS.gene052255.t1
20889 CDS 647 728 647 ID=cds.MS.gene052255.t1;Parent=MS.gene052255.t1
20889 exon 360 430 360 ID=MS.gene052255.t1.exon5;Parent=MS.gene052255.t1
20889 CDS 360 430 360 ID=cds.MS.gene052255.t1;Parent=MS.gene052255.t1
20889 exon 150 229 150 ID=MS.gene052255.t1.exon6;Parent=MS.gene052255.t1
20889 CDS 150 229 150 ID=cds.MS.gene052255.t1;Parent=MS.gene052255.t1
Gene Sequence

>MS.gene052255

ATGGCTATTTCAATATTAAGAGCTGTAATGGTATCTAATTCTCATTGTCAATCTCAACCACACATTCATAACATATCTTCTATTGATGAAAATGCTAAAAGGAGGAAAAGAGTAGTTTTCTCTTCTCATTCTTCAGAACTTAACCCTGTAATTAGGTTAGTAAACTCTTCATTTTTTGTTTAACTATTAATGCTTAATACTTTTTTTAGGTCTATGTTAGAATTGGGTTTTGGTTTTACTTTATAGGTCATCAGAGGGTTTCATTTTCTGTTGGGACTTGCCTTATTCCACATCCAAAAAAGGTAATAGTAATTATATAGTTAACACTAGTTACATGTTGTTTTGTGTATTTGTTACTTGTTAGAGACTTAGAGTTGTGGTGTTTCTTGAAGGTTGAGAAAGGTGGGGAAGATGCTTTCTTTGTGAGCAACTATAATGGTGGAGTTATTGCTGTTGCTGATGGTGTCTCTGGGTATGCTACTTACTCACTAATTCATTATTGGCAAAAAACTACTTTCAGATTCATTGAACAACTTATGTATCTTGTCTATAATATTATAGACCAGATACATCATTTATTAGTTGAATCTAAAAAGTTGTTTTTTGCTTATAATAGTGACCAGAAGGTGTAAGTTTTTATATGATAAACAAATATTATAAGCTTATATGATAAAAAAATGTTTAATTAAATTGTTTATCCAAACAAGGCATTACTATCATTAAACAAACACAAAATGACACCCTGACTAAAATCTTGCTTTGTTTTTCAGTTATAAATTTAGATGGTGTTGTTATGCATCATATTGAGATCTTGTCTTGTGACAATTTGTCTCAACGTGTTGTTGTGTTATGCTTCACAGTTGGGCTGAAGAGGATGTAGATCCTTCATTATTCCCACGTGAATTGATGGCTAATGCTTATAATTTTGTACAAGATGAGGAGGTACCGTACCCTAAACGCAGATACTGATTATGACTATTCGTAGAATAACATTATATTTGCTTTCCAGTTACATTCATTCTTTTGAAATTTAGAATGATATCTGTTTTTTATCTTTAAATTTTGAGCTGTAATTCAATAAGAATGGTTATATGCATGTTGTTTGGTGACAAAAGTAAACAAACATGTTGATTCGTTGAATATTGGTTGATTTTGCAGGTGAATAATGATCCTCAGATTCTTATAAGGAAAGCACATGCTGCTACATTCTCTACAGGCTCAGCAACTGTGTAAGTTTATCCTCTTTTATTTAGGTTGCTAATTTGGAGTAAAAAGATCAAACATTGCTATGCATTTTAATATTCAAACTATTCTACCACAATATAGCCTCAACAATTTCTTGTCTGTGATTCTGTGAAGCATCATTGCTATGCTGGAAAAGAATGGGAATCTAAAGATTGCCAATGTTGGTGATTGTGGATTACGAGTTATCCGTAATG

Protein sequence

>MS.gene052255.t1

MAISILRAVMVSNSHCQSQPHIHNISSIDENAKRRKRVVFSSHSSELNPVIRSSEGFIFCWDLPYSTSKKGGEDAFFVSNYNGGVIAVADGVSGWAEEDVDPSLFPRELMANAYNFVQDEEVNNDPQILIRKAHAATFSTGSATVIIAMLEKNGNLKIANVGDCGLRVIRN