Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065054.t1 | XP_013470277.1 | 89.6 | 115 | 11 | 1 | 1 | 115 | 183 | 296 | 1.90E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065054.t1 | A0A072W228 | 89.6 | 115 | 11 | 1 | 1 | 115 | 183 | 296 | 1.3e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene065054 | 0.804803 | 1.81E-49 | -1.69E-46 |
| MS.gene051265 | MS.gene065054 | 0.816434 | 5.54E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene065054 | 0.825914 | 3.61E-54 | -1.69E-46 |
| MS.gene05289 | MS.gene065054 | 0.812458 | 4.20E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene065054 | 0.824408 | 8.20E-54 | -1.69E-46 |
| MS.gene053184 | MS.gene065054 | 0.804847 | 1.77E-49 | -1.69E-46 |
| MS.gene054184 | MS.gene065054 | 0.809567 | 1.77E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene065054 | 0.81199 | 5.31E-51 | -1.69E-46 |
| MS.gene054713 | MS.gene065054 | 0.882916 | 6.97E-71 | -1.69E-46 |
| MS.gene055356 | MS.gene065054 | 0.808606 | 2.85E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene065054 | 0.808903 | 2.46E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene065054 | 0.815053 | 1.13E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene065054 | 0.837027 | 6.62E-57 | -1.69E-46 |
| MS.gene055860 | MS.gene065054 | 0.810082 | 1.38E-50 | -1.69E-46 |
| MS.gene058042 | MS.gene065054 | 0.855691 | 5.32E-62 | -1.69E-46 |
| MS.gene058168 | MS.gene065054 | 0.822658 | 2.11E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene065054 | -0.815509 | 8.91E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065054.t1 | MTR_1g112150 | 89.565 | 115 | 11 | 1 | 1 | 115 | 183 | 296 | 1.50e-71 | 215 |
| MS.gene065054.t1 | MTR_1g112160 | 64.706 | 119 | 41 | 1 | 1 | 119 | 134 | 251 | 7.03e-49 | 156 |
| MS.gene065054.t1 | MTR_2g021690 | 47.312 | 93 | 48 | 1 | 3 | 94 | 188 | 280 | 4.06e-20 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065054.t1 | AT1G55240 | 42.000 | 100 | 58 | 0 | 3 | 102 | 190 | 289 | 2.06e-25 | 97.4 |
| MS.gene065054.t1 | AT1G55230 | 41.905 | 105 | 60 | 1 | 1 | 105 | 188 | 291 | 4.67e-25 | 96.3 |
| MS.gene065054.t1 | AT5G19870 | 47.727 | 88 | 46 | 0 | 3 | 90 | 189 | 276 | 3.57e-24 | 93.6 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATTATGGCTATAATTTA+TGG | 0.111898 | 1.2:+82960937 | MS.gene065054:CDS |
| CAATTCAGCTGGTTCTTTAT+TGG | 0.239215 | 1.2:+82960886 | MS.gene065054:CDS |
| GGTTTAGTGCTTTCTATATC+TGG | 0.242800 | 1.2:+82960760 | MS.gene065054:CDS |
| TCAACCCAAAGGTTGCTTCT+TGG | 0.301483 | 1.2:+82960786 | MS.gene065054:CDS |
| AGGTTTGTTCATTATGTTCA+TGG | 0.326708 | 1.2:+82960738 | MS.gene065054:CDS |
| AGTGTCTTTCTACTTGATTA+TGG | 0.354291 | 1.2:+82960924 | MS.gene065054:CDS |
| ATATTCCAAGAAGCAACCTT+TGG | 0.390404 | 1.2:-82960791 | None:intergenic |
| AAGGAGATCAACTTATGATT+AGG | 0.413259 | 1.2:+82960815 | MS.gene065054:CDS |
| CCAGATGATTTCTACTAGAA+TGG | 0.432826 | 1.2:+82961050 | MS.gene065054:CDS |
| TGGTGAATCTTCAATTCAGC+TGG | 0.442803 | 1.2:+82960875 | MS.gene065054:CDS |
| CCATTCTAGTAGAAATCATC+TGG | 0.450188 | 1.2:-82961050 | None:intergenic |
| GGTTTGTTCATTATGTTCAT+GGG | 0.458171 | 1.2:+82960739 | MS.gene065054:CDS |
| TATTCCAAGAAGCAACCTTT+GGG | 0.461299 | 1.2:-82960790 | None:intergenic |
| TATGCATCGTGCTGTTTCAT+TGG | 0.468772 | 1.2:+82960855 | MS.gene065054:CDS |
| GATTAGGTGCAATGATGAAA+AGG | 0.487514 | 1.2:+82960831 | MS.gene065054:CDS |
| GGTTGCTTCTTGGAATATGA+AGG | 0.488311 | 1.2:+82960796 | MS.gene065054:CDS |
| TGAACATAATGAACAAACCT+TGG | 0.523714 | 1.2:-82960735 | None:intergenic |
| TCTGTTAGCATGATCTTCCA+AGG | 0.532759 | 1.2:+82960718 | None:intergenic |
| GGAGTGCGATTCACTGATGA+AGG | 0.536386 | 1.2:+82960969 | MS.gene065054:CDS |
| AAGGAGGAACAGTACGGTGA+AGG | 0.576386 | 1.2:+82960988 | MS.gene065054:CDS |
| GAACAGTACGGTGAAGGCGA+TGG | 0.598192 | 1.2:+82960994 | MS.gene065054:CDS |
| ATATCTGGTTATCAACCCAA+AGG | 0.602519 | 1.2:+82960775 | MS.gene065054:CDS |
| GTGCGATTCACTGATGAAGG+AGG | 0.666959 | 1.2:+82960972 | MS.gene065054:CDS |
| CTGATGAAGGAGGAACAGTA+CGG | 0.675878 | 1.2:+82960982 | MS.gene065054:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAATTTATGGTGAAAAAG+TGG | + | chr1.2:82960948-82960967 | MS.gene065054:CDS | 20.0% |
| !! | TTGATTATGGCTATAATTTA+TGG | + | chr1.2:82960937-82960956 | MS.gene065054:CDS | 20.0% |
| AAGGAGATCAACTTATGATT+AGG | + | chr1.2:82960815-82960834 | MS.gene065054:CDS | 30.0% | |
| AGTGTCTTTCTACTTGATTA+TGG | + | chr1.2:82960924-82960943 | MS.gene065054:CDS | 30.0% | |
| TGAACATAATGAACAAACCT+TGG | - | chr1.2:82960738-82960757 | None:intergenic | 30.0% | |
| ! | AGGTTTGTTCATTATGTTCA+TGG | + | chr1.2:82960738-82960757 | MS.gene065054:CDS | 30.0% |
| ! | GGTTTGTTCATTATGTTCAT+GGG | + | chr1.2:82960739-82960758 | MS.gene065054:CDS | 30.0% |
| ATATCTGGTTATCAACCCAA+AGG | + | chr1.2:82960775-82960794 | MS.gene065054:CDS | 35.0% | |
| ATATTCCAAGAAGCAACCTT+TGG | - | chr1.2:82960794-82960813 | None:intergenic | 35.0% | |
| CCAGATGATTTCTACTAGAA+TGG | + | chr1.2:82961050-82961069 | MS.gene065054:CDS | 35.0% | |
| CCATTCTAGTAGAAATCATC+TGG | - | chr1.2:82961053-82961072 | None:intergenic | 35.0% | |
| GATTAGGTGCAATGATGAAA+AGG | + | chr1.2:82960831-82960850 | MS.gene065054:CDS | 35.0% | |
| GGTTTAGTGCTTTCTATATC+TGG | + | chr1.2:82960760-82960779 | MS.gene065054:CDS | 35.0% | |
| TATTCCAAGAAGCAACCTTT+GGG | - | chr1.2:82960793-82960812 | None:intergenic | 35.0% | |
| ! | CAATTCAGCTGGTTCTTTAT+TGG | + | chr1.2:82960886-82960905 | MS.gene065054:CDS | 35.0% |
| GGTTGCTTCTTGGAATATGA+AGG | + | chr1.2:82960796-82960815 | MS.gene065054:CDS | 40.0% | |
| TGGTGAATCTTCAATTCAGC+TGG | + | chr1.2:82960875-82960894 | MS.gene065054:CDS | 40.0% | |
| !! | TATGCATCGTGCTGTTTCAT+TGG | + | chr1.2:82960855-82960874 | MS.gene065054:CDS | 40.0% |
| CTGATGAAGGAGGAACAGTA+CGG | + | chr1.2:82960982-82961001 | MS.gene065054:CDS | 45.0% | |
| TCAACCCAAAGGTTGCTTCT+TGG | + | chr1.2:82960786-82960805 | MS.gene065054:CDS | 45.0% | |
| AAGGAGGAACAGTACGGTGA+AGG | + | chr1.2:82960988-82961007 | MS.gene065054:CDS | 50.0% | |
| GGAGTGCGATTCACTGATGA+AGG | + | chr1.2:82960969-82960988 | MS.gene065054:CDS | 50.0% | |
| GTGCGATTCACTGATGAAGG+AGG | + | chr1.2:82960972-82960991 | MS.gene065054:CDS | 50.0% | |
| GAACAGTACGGTGAAGGCGA+TGG | + | chr1.2:82960994-82961013 | MS.gene065054:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 82960727 | 82961086 | 82960727 | ID=MS.gene065054 |
| chr1.2 | mRNA | 82960727 | 82961086 | 82960727 | ID=MS.gene065054.t1;Parent=MS.gene065054 |
| chr1.2 | exon | 82960727 | 82961086 | 82960727 | ID=MS.gene065054.t1.exon1;Parent=MS.gene065054.t1 |
| chr1.2 | CDS | 82960727 | 82961086 | 82960727 | ID=cds.MS.gene065054.t1;Parent=MS.gene065054.t1 |
| Gene Sequence |
| Protein sequence |
