Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065599.t1 | XP_013467608.1 | 98.9 | 352 | 4 | 0 | 34 | 385 | 33 | 384 | 2.00E-202 | 714.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065599.t1 | Q8S9J2 | 80.5 | 348 | 66 | 1 | 38 | 385 | 40 | 385 | 1.9e-169 | 596.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065599.t1 | A0A072VU52 | 98.9 | 352 | 4 | 0 | 34 | 385 | 33 | 384 | 1.4e-202 | 714.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene065599 | 0.823941 | 1.06E-53 | -1.69E-46 |
| MS.gene050320 | MS.gene065599 | 0.801838 | 7.45E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene065599 | 0.837442 | 5.19E-57 | -1.69E-46 |
| MS.gene050838 | MS.gene065599 | 0.845526 | 3.84E-59 | -1.69E-46 |
| MS.gene050955 | MS.gene065599 | 0.800314 | 1.53E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene065599 | 0.809478 | 1.85E-50 | -1.69E-46 |
| MS.gene051756 | MS.gene065599 | 0.80086 | 1.18E-48 | -1.69E-46 |
| MS.gene051850 | MS.gene065599 | 0.82956 | 4.81E-55 | -1.69E-46 |
| MS.gene051903 | MS.gene065599 | 0.835249 | 1.87E-56 | -1.69E-46 |
| MS.gene052947 | MS.gene065599 | 0.836977 | 6.82E-57 | -1.69E-46 |
| MS.gene053109 | MS.gene065599 | 0.819628 | 1.05E-52 | -1.69E-46 |
| MS.gene053952 | MS.gene065599 | 0.822309 | 2.54E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene065599 | 0.835507 | 1.61E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene065599 | 0.802885 | 4.54E-49 | -1.69E-46 |
| MS.gene056075 | MS.gene065599 | 0.856088 | 4.08E-62 | -1.69E-46 |
| MS.gene056631 | MS.gene065599 | 0.809651 | 1.70E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene065599 | 0.825268 | 5.14E-54 | -1.69E-46 |
| MS.gene057705 | MS.gene065599 | 0.80624 | 9.05E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene065599 | 0.822497 | 2.29E-53 | -1.69E-46 |
| MS.gene061145 | MS.gene065599 | 0.819051 | 1.42E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065599.t1 | MTR_1g053270 | 98.182 | 385 | 6 | 1 | 1 | 385 | 1 | 384 | 0.0 | 779 |
| MS.gene065599.t1 | MTR_1g007340 | 78.421 | 380 | 74 | 2 | 6 | 385 | 1 | 372 | 0.0 | 633 |
| MS.gene065599.t1 | MTR_1g007340 | 66.667 | 108 | 30 | 1 | 7 | 114 | 380 | 481 | 1.97e-37 | 141 |
| MS.gene065599.t1 | MTR_1g007325 | 73.047 | 256 | 49 | 1 | 130 | 385 | 1 | 236 | 1.26e-135 | 387 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065599.t1 | AT2G33840 | 80.460 | 348 | 66 | 1 | 38 | 385 | 40 | 385 | 0.0 | 604 |
| MS.gene065599.t1 | AT1G28350 | 69.705 | 373 | 111 | 1 | 13 | 385 | 1 | 371 | 0.0 | 559 |
| MS.gene065599.t1 | AT1G28350 | 69.921 | 379 | 109 | 3 | 7 | 385 | 375 | 748 | 0.0 | 542 |
| MS.gene065599.t1 | AT1G28350 | 69.705 | 373 | 111 | 1 | 13 | 385 | 1 | 371 | 0.0 | 559 |
| MS.gene065599.t1 | AT1G28350 | 69.921 | 379 | 109 | 3 | 7 | 385 | 375 | 748 | 0.0 | 542 |
| MS.gene065599.t1 | AT1G28350 | 69.921 | 379 | 109 | 3 | 7 | 385 | 309 | 682 | 0.0 | 541 |
| MS.gene065599.t1 | AT1G28350 | 74.267 | 307 | 77 | 1 | 79 | 385 | 1 | 305 | 4.74e-170 | 491 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATACATGGATAGAATATT+TGG | 0.140591 | 1.4:-38963391 | None:intergenic |
| TGCTAAGCTTAATAACAAAA+TGG | 0.199810 | 1.4:+38963042 | MS.gene065599:CDS |
| TTATAGGTGTAGCCAAATTA+TGG | 0.235240 | 1.4:+38963337 | MS.gene065599:intron |
| CTCTTCATCCTCCATGTAAA+TGG | 0.251052 | 1.4:-38964352 | None:intergenic |
| GCCGCTACTTGATCGAAATT+TGG | 0.252289 | 1.4:+38963095 | MS.gene065599:CDS |
| GTTCACTCCGTCCCATAATT+TGG | 0.255447 | 1.4:-38963349 | None:intergenic |
| AATTACTGCGGTGCTGGATT+TGG | 0.273627 | 1.4:-38957037 | None:intergenic |
| ACTTCGACAAAGCCGGTTTA+AGG | 0.288565 | 1.4:-38965061 | None:intergenic |
| GGGTTTCCTTCCACAATCTT+TGG | 0.305525 | 1.4:-38964735 | None:intergenic |
| TGCGGTGCTGGATTTGGATT+TGG | 0.330970 | 1.4:-38957031 | None:intergenic |
| ACAACCTTAAAAGAATTATC+AGG | 0.345241 | 1.4:+38963233 | MS.gene065599:CDS |
| CCTCAGCCTGTTTGTTATGA+TGG | 0.349699 | 1.4:+38957229 | MS.gene065599:CDS |
| TCCATGTAAATGGATGATAA+AGG | 0.366918 | 1.4:-38964342 | None:intergenic |
| CCCTTTATCATCCATTTACA+TGG | 0.371480 | 1.4:+38964341 | MS.gene065599:CDS |
| CTGGTTGCCTAGTCAAAATA+TGG | 0.374269 | 1.4:+38963005 | MS.gene065599:CDS |
| AGTATTGTGATGTTATTAAG+AGG | 0.375155 | 1.4:+38964161 | MS.gene065599:CDS |
| TCAAAATATGGATTGCTGAT+TGG | 0.384798 | 1.4:+38963017 | MS.gene065599:CDS |
| TTGAAGAAAATTGAGATTGT+TGG | 0.389262 | 1.4:+38963073 | MS.gene065599:CDS |
| TATAGGTGTAGCCAAATTAT+GGG | 0.399258 | 1.4:+38963338 | MS.gene065599:intron |
| GCTAAGCTTAATAACAAAAT+GGG | 0.426202 | 1.4:+38963043 | MS.gene065599:CDS |
| TTCTCTGATCCATTCCCAAT+TGG | 0.433325 | 1.4:-38964117 | None:intergenic |
| ATAGGCCGACATCTGCCAAT+TGG | 0.434224 | 1.4:+38964102 | MS.gene065599:intron |
| AGCCGCCGCCGCAGTAGCAC+TGG | 0.438411 | 1.4:+38956973 | MS.gene065599:CDS |
| CCATGTAAATGGATGATAAA+GGG | 0.439796 | 1.4:-38964341 | None:intergenic |
| TCGAAATTTGGAAAGCCGTT+GGG | 0.440768 | 1.4:+38963107 | MS.gene065599:CDS |
| TTGGAAATATGCAGCCTGTA+AGG | 0.446216 | 1.4:+38966139 | MS.gene065599:intron |
| CATACATGGATAGAATATTT+GGG | 0.452640 | 1.4:-38963390 | None:intergenic |
| GAAAGCCGTTGGGATGGATC+TGG | 0.454876 | 1.4:+38963117 | MS.gene065599:CDS |
| TTAAATATCTTATCTTACCT+TGG | 0.454939 | 1.4:+38964772 | MS.gene065599:CDS |
| TAGGCCGACATCTGCCAATT+GGG | 0.459139 | 1.4:+38964103 | MS.gene065599:intron |
| ATCGAAATTTGGAAAGCCGT+TGG | 0.468318 | 1.4:+38963106 | MS.gene065599:CDS |
| TCAAAACCATCATAACAAAC+AGG | 0.473991 | 1.4:-38957235 | None:intergenic |
| ACATCCAGCTGACCTTAAAC+CGG | 0.474408 | 1.4:+38965049 | MS.gene065599:CDS |
| ATGTCAGCACACTGCATACA+TGG | 0.480237 | 1.4:-38963404 | None:intergenic |
| TCTATAATCAACACATTACT+TGG | 0.482569 | 1.4:-38966334 | None:intergenic |
| TGGACGAATGCACATTGCTC+AGG | 0.484195 | 1.4:+38957264 | MS.gene065599:CDS |
| TCTTGCCCTTGTTGTAGACC+AGG | 0.488474 | 1.4:-38964300 | None:intergenic |
| TTTCCTTCCACAATCTTTGG+CGG | 0.490487 | 1.4:-38964732 | None:intergenic |
| TTCAATGACTTCGACAAAGC+CGG | 0.497005 | 1.4:-38965068 | None:intergenic |
| TGAGTATTGGCCTCTTGTGT+TGG | 0.497158 | 1.4:+38963195 | MS.gene065599:CDS |
| GTGGAGCGTAATGCAGATAA+TGG | 0.504803 | 1.4:+38964810 | MS.gene065599:CDS |
| TGCTGGATTTGGATTTGGAT+TGG | 0.505166 | 1.4:-38957026 | None:intergenic |
| TTCCTTCCACAATCTTTGGC+GGG | 0.512138 | 1.4:-38964731 | None:intergenic |
| TGTGGAAGGAAACCCATGCT+TGG | 0.518682 | 1.4:+38964743 | MS.gene065599:CDS |
| ATGCCAGAGCTGATGAGTAT+TGG | 0.524296 | 1.4:+38963182 | MS.gene065599:CDS |
| TATTTAATGTACTCCAAGCA+TGG | 0.526777 | 1.4:-38964756 | None:intergenic |
| AATAACATCACAATACTCTC+TGG | 0.528355 | 1.4:-38964155 | None:intergenic |
| ATTTAATGTACTCCAAGCAT+GGG | 0.531281 | 1.4:-38964755 | None:intergenic |
| TTCCAGTGCTACTGCGGCGG+CGG | 0.533461 | 1.4:-38956975 | None:intergenic |
| CGACATCTGCCAATTGGGAA+TGG | 0.537270 | 1.4:+38964108 | MS.gene065599:CDS |
| TTTGAGATCGTGAGAAGCAT+CGG | 0.543232 | 1.4:+38957157 | MS.gene065599:CDS |
| AAAGCCGGTTTAAGGTCAGC+TGG | 0.544325 | 1.4:-38965053 | None:intergenic |
| AGGCCAATACTCATCAGCTC+TGG | 0.554004 | 1.4:-38963185 | None:intergenic |
| CATTTACATGGAGGATGAAG+AGG | 0.563565 | 1.4:+38964353 | MS.gene065599:CDS |
| TAACTCTCCGCTTTCATAGT+CGG | 0.564786 | 1.4:-38965027 | None:intergenic |
| AATTTGGAAAGCCGTTGGGA+TGG | 0.569257 | 1.4:+38963111 | MS.gene065599:CDS |
| TCCAAATTTCGATCAAGTAG+CGG | 0.571639 | 1.4:-38963096 | None:intergenic |
| TTACGGTGTGATAAGATGAC+GGG | 0.572281 | 1.4:-38964193 | None:intergenic |
| CATTCCCAATTGGCAGATGT+CGG | 0.585557 | 1.4:-38964107 | None:intergenic |
| ATGTTACCTGGTCTACAACA+AGG | 0.591222 | 1.4:+38964294 | MS.gene065599:CDS |
| AAGCTTAATAACAAAATGGG+TGG | 0.593404 | 1.4:+38963046 | MS.gene065599:CDS |
| CCATCATAACAAACAGGCTG+AGG | 0.595061 | 1.4:-38957229 | None:intergenic |
| TGCCCGCCAAAGATTGTGGA+AGG | 0.597557 | 1.4:+38964729 | MS.gene065599:CDS |
| ATATTGCCCGCCAAAGATTG+TGG | 0.598009 | 1.4:+38964725 | MS.gene065599:CDS |
| GTGAACAAGCTAACATCCGC+TGG | 0.599305 | 1.4:+38962986 | MS.gene065599:CDS |
| GTCATTGAATAAAATTCTAG+AGG | 0.600216 | 1.4:+38965082 | MS.gene065599:CDS |
| TCCTTCCAGATCCATCCCAA+CGG | 0.609022 | 1.4:-38963122 | None:intergenic |
| CAATGATTCCAGTGCTACTG+CGG | 0.621909 | 1.4:-38956981 | None:intergenic |
| ATTACGGTGTGATAAGATGA+CGG | 0.622032 | 1.4:-38964194 | None:intergenic |
| GCAATGTGCATTCGTCCAGA+AGG | 0.623405 | 1.4:-38957259 | None:intergenic |
| GTTGGGATGGATCTGGAAGG+AGG | 0.625900 | 1.4:+38963124 | MS.gene065599:CDS |
| TTGATTGCCGACTATGAAAG+CGG | 0.628637 | 1.4:+38965020 | MS.gene065599:CDS |
| CATGCATAGGTTGTTGACTG+CGG | 0.633578 | 1.4:+38957126 | MS.gene065599:intron |
| TGTTACCTGGTCTACAACAA+GGG | 0.634319 | 1.4:+38964295 | MS.gene065599:CDS |
| TGAGCTATATCCAACACAAG+AGG | 0.641928 | 1.4:-38963205 | None:intergenic |
| TTTATCATCCATTTACATGG+AGG | 0.642941 | 1.4:+38964344 | MS.gene065599:CDS |
| TTGGTTTAACGAGTTTACTG+TGG | 0.643574 | 1.4:+38964791 | MS.gene065599:CDS |
| TGATTCCAGTGCTACTGCGG+CGG | 0.646365 | 1.4:-38956978 | None:intergenic |
| GGTGTAGCCAAATTATGGGA+CGG | 0.659015 | 1.4:+38963342 | MS.gene065599:intron |
| GGTAGATGATGATTCAGCGA+CGG | 0.660169 | 1.4:-38957005 | None:intergenic |
| GCCGTTGGGATGGATCTGGA+AGG | 0.662319 | 1.4:+38963121 | MS.gene065599:CDS |
| ACAGTAAACTCGTTAAACCA+AGG | 0.685168 | 1.4:-38964789 | None:intergenic |
| GAGCGTAATGCAGATAATGG+TGG | 0.730139 | 1.4:+38964813 | MS.gene065599:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 38956956 | 38966341 | 38956956 | ID=MS.gene065599 |
| chr1.4 | mRNA | 38956956 | 38966341 | 38956956 | ID=MS.gene065599.t1;Parent=MS.gene065599 |
| chr1.4 | exon | 38956956 | 38957053 | 38956956 | ID=MS.gene065599.t1.exon1;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38956956 | 38957053 | 38956956 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38957135 | 38957285 | 38957135 | ID=MS.gene065599.t1.exon2;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38957135 | 38957285 | 38957135 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38962965 | 38963254 | 38962965 | ID=MS.gene065599.t1.exon3;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38962965 | 38963254 | 38962965 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38963343 | 38963439 | 38963343 | ID=MS.gene065599.t1.exon4;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38963343 | 38963439 | 38963343 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38964106 | 38964211 | 38964106 | ID=MS.gene065599.t1.exon5;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38964106 | 38964211 | 38964106 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38964292 | 38964374 | 38964292 | ID=MS.gene065599.t1.exon6;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38964292 | 38964374 | 38964292 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38964696 | 38964841 | 38964696 | ID=MS.gene065599.t1.exon7;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38964696 | 38964841 | 38964696 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38964998 | 38965103 | 38964998 | ID=MS.gene065599.t1.exon8;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38964998 | 38965103 | 38964998 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38966153 | 38966212 | 38966153 | ID=MS.gene065599.t1.exon9;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38966153 | 38966212 | 38966153 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| chr1.4 | exon | 38966321 | 38966341 | 38966321 | ID=MS.gene065599.t1.exon10;Parent=MS.gene065599.t1 |
| chr1.4 | CDS | 38966321 | 38966341 | 38966321 | ID=cds.MS.gene065599.t1;Parent=MS.gene065599.t1 |
| Gene Sequence |
| Protein sequence |
