AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067085


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067085.t1 MTR_3g086590 90.909 209 4 4 1 209 1 194 5.46e-135 376
MS.gene067085.t1 MTR_4g104680 57.037 135 56 2 75 209 58 190 1.81e-46 162
MS.gene067085.t1 MTR_1g047990 34.109 129 82 1 75 203 78 203 2.37e-17 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067085.t1 AT1G67810 50.532 188 85 5 25 209 27 209 2.17e-56 179
MS.gene067085.t1 AT5G50210 48.148 135 68 1 75 209 87 219 3.60e-37 137
MS.gene067085.t1 AT4G26500 35.659 129 80 1 75 203 86 211 6.49e-17 78.6

Find 70 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACACAGGATTTAATTTA+AGG 0.116156 3.4:+80656433 MS.gene067085:CDS
GAGAAGGAGGGAGGGTTAAT+AGG 0.172032 3.4:-80655874 None:intergenic
AACCCGGTCGGACCGGTCTA+AGG 0.264863 3.4:-80656137 None:intergenic
ATAATGTTAAAGCACGTTCT+AGG 0.299172 3.4:+80656371 MS.gene067085:CDS
CAGGTTGTTCGACAGATGTT+TGG 0.324183 3.4:+80656176 MS.gene067085:CDS
AGAAGGAGGGAGGGTTAATA+GGG 0.328208 3.4:-80655873 None:intergenic
GGTTCTGTTGGTGTCTTGTT+TGG 0.336055 3.4:+80656269 MS.gene067085:CDS
AACGGCGGCGGCGTTAAGTT+TGG 0.345006 3.4:-80655918 None:intergenic
TAACGTCGAGAAAGATGTTT+TGG 0.349720 3.4:+80656324 MS.gene067085:CDS
AGACCGGTCCGACCGGGTTC+CGG 0.370178 3.4:+80656141 MS.gene067085:CDS
TTCTCCTACCTTTGTCTATG+TGG 0.388043 3.4:-80656207 None:intergenic
CTCCCTTAGACCGGTCCGAC+CGG 0.409468 3.4:+80656134 MS.gene067085:CDS
CCGACCGGGTTCCGGAGAAC+CGG 0.419653 3.4:+80656149 MS.gene067085:CDS
TTGGTTGGTATGAATGTAGT+AGG 0.419990 3.4:+80656343 MS.gene067085:CDS
CAACCTGTCACCCGGTTCTC+CGG 0.423018 3.4:-80656160 None:intergenic
GTTGAAGGGAGGAGGGTTAA+CGG 0.424094 3.4:-80655969 None:intergenic
GTTGGGAATGGAAATGGAAA+TGG 0.446723 3.4:-80655833 None:intergenic
AGGTTGTTCGACAGATGTTT+GGG 0.451276 3.4:+80656177 MS.gene067085:CDS
TGTCTTGTTTGGATGTTTGA+CGG 0.452263 3.4:+80656280 MS.gene067085:CDS
AAGGAGAAGGAGAAGGAGGG+AGG 0.455202 3.4:-80655883 None:intergenic
TGAGAAGGAGAAGGAGAAGG+AGG 0.464083 3.4:-80655887 None:intergenic
GTTCCGGAGAACCGGGTGAC+AGG 0.466591 3.4:+80656157 MS.gene067085:CDS
GTGGAACTTGCCGTAGAAGT+TGG 0.470590 3.4:-80655851 None:intergenic
GTAGAAGTTGGGAATGGAAA+TGG 0.471303 3.4:-80655839 None:intergenic
TGGTTGGTATGAATGTAGTA+GGG 0.480747 3.4:+80656344 MS.gene067085:CDS
AGGAGAAGGAGAAGGAGGGA+GGG 0.480933 3.4:-80655882 None:intergenic
TGGAACTTGCCGTAGAAGTT+GGG 0.482966 3.4:-80655850 None:intergenic
TCTCCTACCTTTGTCTATGT+GGG 0.491686 3.4:-80656206 None:intergenic
ATTTCCATTCCCAACTTCTA+CGG 0.492903 3.4:+80655841 MS.gene067085:CDS
TCTCCGGAACCCGGTCGGAC+CGG 0.495621 3.4:-80656144 None:intergenic
CTTTATTGCCTCCCTTAGAC+CGG 0.497345 3.4:+80656125 MS.gene067085:CDS
TTCGACAGATGTTTGGGTGG+TGG 0.504447 3.4:+80656183 MS.gene067085:CDS
TCGACGTTAAGCACTTCCTC+AGG 0.505903 3.4:-80656310 None:intergenic
TCCCTTAGACCGGTCCGACC+GGG 0.506418 3.4:+80656135 MS.gene067085:CDS
AATGTGTTGTTGAAGGGAGG+AGG 0.512295 3.4:-80655977 None:intergenic
ATGTGTTGTTGAAGGGAGGA+GGG 0.516128 3.4:-80655976 None:intergenic
GAGAAGGAGAAGGAGAAGGA+GGG 0.521011 3.4:-80655886 None:intergenic
GTTTCAAGCAGACAGCGATT+CGG 0.525213 3.4:+80656234 MS.gene067085:CDS
GATGTTAATGTGTTGTTGAA+GGG 0.535061 3.4:-80655983 None:intergenic
GGCGGCGGCGTTAAGTTTGG+AGG 0.536844 3.4:-80655915 None:intergenic
CGACCGGGTTCCGGAGAACC+GGG 0.537863 3.4:+80656150 MS.gene067085:CDS
TGATGTTAATGTGTTGTTGA+AGG 0.542600 3.4:-80655984 None:intergenic
AGGAGGGAGGGTTAATAGGG+TGG 0.544493 3.4:-80655870 None:intergenic
AAATGAGAAGGAGAAGGAGA+AGG 0.545044 3.4:-80655890 None:intergenic
ACCCGGTCGGACCGGTCTAA+GGG 0.546406 3.4:-80656136 None:intergenic
AGTTTGGAGGAGAAATGAGA+AGG 0.551683 3.4:-80655902 None:intergenic
TAGACAAAGGTAGGAGAATG+AGG 0.563185 3.4:+80656212 MS.gene067085:CDS
TGCCATGCAAAAGGCTACAC+AGG 0.565139 3.4:+80656420 MS.gene067085:CDS
TCACCCGGTTCTCCGGAACC+CGG 0.567326 3.4:-80656153 None:intergenic
GAGGAGAAATGAGAAGGAGA+AGG 0.570126 3.4:-80655896 None:intergenic
CTGTCGAACAACCTGTCACC+CGG 0.571190 3.4:-80656168 None:intergenic
GGTTAACGGAGAGTTTGAGA+TGG 0.571772 3.4:-80655955 None:intergenic
AGATATCGAAGGGGTTCTGT+TGG 0.579062 3.4:+80656257 MS.gene067085:CDS
GTCGATGGGTTCGGAGAGAG+AGG 0.581664 3.4:-80656074 None:intergenic
GTTTGACGGCGCGGAACCTG+AGG 0.591156 3.4:+80656294 MS.gene067085:CDS
CCGGTTCTCCGGAACCCGGT+CGG 0.599576 3.4:-80656149 None:intergenic
AGCGATTCGGAGATATCGAA+GGG 0.599661 3.4:+80656247 MS.gene067085:CDS
CAGCGATTCGGAGATATCGA+AGG 0.616023 3.4:+80656246 MS.gene067085:CDS
TGGCCCACATAGACAAAGGT+AGG 0.620651 3.4:+80656203 MS.gene067085:CDS
GGAGAGAGAGGCAAATTCGA+TGG 0.622274 3.4:-80656062 None:intergenic
TCATCATCACCGTCATCTCA+CGG 0.622993 3.4:+80656013 MS.gene067085:CDS
CTTGCCGTAGAAGTTGGGAA+TGG 0.632430 3.4:-80655845 None:intergenic
TTGTTCGACAGATGTTTGGG+TGG 0.634015 3.4:+80656180 MS.gene067085:CDS
CGGTCGGACCGGTCTAAGGG+AGG 0.638651 3.4:-80656133 None:intergenic
GTTAATGTGTTGTTGAAGGG+AGG 0.644344 3.4:-80655980 None:intergenic
TGTTTGGATGTTTGACGGCG+CGG 0.685933 3.4:+80656285 MS.gene067085:CDS
GTGGTGGCCCACATAGACAA+AGG 0.687074 3.4:+80656199 MS.gene067085:CDS
TCAGTGACACCGTGAGATGA+CGG 0.694277 3.4:-80656022 None:intergenic
CACGTTCTAGGATTAACACG+TGG 0.721590 3.4:+80656383 MS.gene067085:CDS
GCGATTCGGAGATATCGAAG+GGG 0.771683 3.4:+80656248 MS.gene067085:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTAATAGTCTTTTTACT+CGG - chr3.4:80656099-80656118 None:intergenic 20.0%
!!! ATGGATTTGTTTTGAAGTAA+CGG - chr3.4:80655939-80655958 None:intergenic 25.0%
ATAATGTTAAAGCACGTTCT+AGG + chr3.4:80656371-80656390 MS.gene067085:CDS 30.0%
! GATGTTAATGTGTTGTTGAA+GGG - chr3.4:80655986-80656005 None:intergenic 30.0%
! TATTTTCTTTGCCATGCAAA+AGG + chr3.4:80656411-80656430 MS.gene067085:CDS 30.0%
! TGATGTTAATGTGTTGTTGA+AGG - chr3.4:80655987-80656006 None:intergenic 30.0%
ATTTCCATTCCCAACTTCTA+CGG + chr3.4:80655841-80655860 MS.gene067085:CDS 35.0%
! TAACGTCGAGAAAGATGTTT+TGG + chr3.4:80656324-80656343 MS.gene067085:CDS 35.0%
! TGGTTGGTATGAATGTAGTA+GGG + chr3.4:80656344-80656363 MS.gene067085:CDS 35.0%
! TTGGTTGGTATGAATGTAGT+AGG + chr3.4:80656343-80656362 MS.gene067085:CDS 35.0%
!! TGTCTTGTTTGGATGTTTGA+CGG + chr3.4:80656280-80656299 MS.gene067085:CDS 35.0%
!!! GATTTGTTTTGAAGTAACGG+CGG - chr3.4:80655936-80655955 None:intergenic 35.0%
AAATGAGAAGGAGAAGGAGA+AGG - chr3.4:80655893-80655912 None:intergenic 40.0%
AGGTTGTTCGACAGATGTTT+GGG + chr3.4:80656177-80656196 MS.gene067085:CDS 40.0%
AGTTTGGAGGAGAAATGAGA+AGG - chr3.4:80655905-80655924 None:intergenic 40.0%
GTAGAAGTTGGGAATGGAAA+TGG - chr3.4:80655842-80655861 None:intergenic 40.0%
GTTGGGAATGGAAATGGAAA+TGG - chr3.4:80655836-80655855 None:intergenic 40.0%
TAGACAAAGGTAGGAGAATG+AGG + chr3.4:80656212-80656231 MS.gene067085:CDS 40.0%
TCTCCTACCTTTGTCTATGT+GGG - chr3.4:80656209-80656228 None:intergenic 40.0%
TTCTCCTACCTTTGTCTATG+TGG - chr3.4:80656210-80656229 None:intergenic 40.0%
! AGTCTTTTTACTCGGTCGAT+GGG - chr3.4:80656091-80656110 None:intergenic 40.0%
! GTTAATGTGTTGTTGAAGGG+AGG - chr3.4:80655983-80656002 None:intergenic 40.0%
! TAGTCTTTTTACTCGGTCGA+TGG - chr3.4:80656092-80656111 None:intergenic 40.0%
!!! GTCGAGAAAGATGTTTTGGT+TGG + chr3.4:80656328-80656347 MS.gene067085:CDS 40.0%
AGAAGGAGGGAGGGTTAATA+GGG - chr3.4:80655876-80655895 None:intergenic 45.0%
AGATATCGAAGGGGTTCTGT+TGG + chr3.4:80656257-80656276 MS.gene067085:CDS 45.0%
CAGGTTGTTCGACAGATGTT+TGG + chr3.4:80656176-80656195 MS.gene067085:CDS 45.0%
CTTTATTGCCTCCCTTAGAC+CGG + chr3.4:80656125-80656144 MS.gene067085:CDS 45.0%
GAGGAGAAATGAGAAGGAGA+AGG - chr3.4:80655899-80655918 None:intergenic 45.0%
GGTTAACGGAGAGTTTGAGA+TGG - chr3.4:80655958-80655977 None:intergenic 45.0%
GTTTCAAGCAGACAGCGATT+CGG + chr3.4:80656234-80656253 MS.gene067085:CDS 45.0%
TCATCATCACCGTCATCTCA+CGG + chr3.4:80656013-80656032 MS.gene067085:CDS 45.0%
TGGAACTTGCCGTAGAAGTT+GGG - chr3.4:80655853-80655872 None:intergenic 45.0%
TTGTTCGACAGATGTTTGGG+TGG + chr3.4:80656180-80656199 MS.gene067085:CDS 45.0%
! AATGTGTTGTTGAAGGGAGG+AGG - chr3.4:80655980-80655999 None:intergenic 45.0%
! AGCGATTCGGAGATATCGAA+GGG + chr3.4:80656247-80656266 MS.gene067085:CDS 45.0%
! ATCCTGTGTAGCCTTTTGCA+TGG - chr3.4:80656425-80656444 None:intergenic 45.0%
! ATGTGTTGTTGAAGGGAGGA+GGG - chr3.4:80655979-80655998 None:intergenic 45.0%
! GGTTCTGTTGGTGTCTTGTT+TGG + chr3.4:80656269-80656288 MS.gene067085:CDS 45.0%
!! CACGTTCTAGGATTAACACG+TGG + chr3.4:80656383-80656402 MS.gene067085:CDS 45.0%
!! TTTTACTCGGTCGATGGGTT+CGG - chr3.4:80656086-80656105 None:intergenic 45.0%
!!! TTGTTTTGAAGTAACGGCGG+CGG - chr3.4:80655933-80655952 None:intergenic 45.0%
CTTGCCGTAGAAGTTGGGAA+TGG - chr3.4:80655848-80655867 None:intergenic 50.0%
GAGAAGGAGAAGGAGAAGGA+GGG - chr3.4:80655889-80655908 None:intergenic 50.0%
GAGAAGGAGGGAGGGTTAAT+AGG - chr3.4:80655877-80655896 None:intergenic 50.0%
GGAGAGAGAGGCAAATTCGA+TGG - chr3.4:80656065-80656084 None:intergenic 50.0%
GTGGAACTTGCCGTAGAAGT+TGG - chr3.4:80655854-80655873 None:intergenic 50.0%
GTTGAAGGGAGGAGGGTTAA+CGG - chr3.4:80655972-80655991 None:intergenic 50.0%
TCAGTGACACCGTGAGATGA+CGG - chr3.4:80656025-80656044 None:intergenic 50.0%
TCGACGTTAAGCACTTCCTC+AGG - chr3.4:80656313-80656332 None:intergenic 50.0%
TGAGAAGGAGAAGGAGAAGG+AGG - chr3.4:80655890-80655909 None:intergenic 50.0%
TGCCATGCAAAAGGCTACAC+AGG + chr3.4:80656420-80656439 MS.gene067085:CDS 50.0%
TGGCCCACATAGACAAAGGT+AGG + chr3.4:80656203-80656222 MS.gene067085:CDS 50.0%
TGTTTGGATGTTTGACGGCG+CGG + chr3.4:80656285-80656304 MS.gene067085:CDS 50.0%
TTCGACAGATGTTTGGGTGG+TGG + chr3.4:80656183-80656202 MS.gene067085:CDS 50.0%
! CAGCGATTCGGAGATATCGA+AGG + chr3.4:80656246-80656265 MS.gene067085:CDS 50.0%
! GCGATTCGGAGATATCGAAG+GGG + chr3.4:80656248-80656267 MS.gene067085:CDS 50.0%
AAGGAGAAGGAGAAGGAGGG+AGG - chr3.4:80655886-80655905 None:intergenic 55.0%
AGGAGAAGGAGAAGGAGGGA+GGG - chr3.4:80655885-80655904 None:intergenic 55.0%
AGGAGGGAGGGTTAATAGGG+TGG - chr3.4:80655873-80655892 None:intergenic 55.0%
CTGTCGAACAACCTGTCACC+CGG - chr3.4:80656171-80656190 None:intergenic 55.0%
GTGGTGGCCCACATAGACAA+AGG + chr3.4:80656199-80656218 MS.gene067085:CDS 55.0%
AACGGCGGCGGCGTTAAGTT+TGG - chr3.4:80655921-80655940 None:intergenic 60.0%
CAACCTGTCACCCGGTTCTC+CGG - chr3.4:80656163-80656182 None:intergenic 60.0%
! GTCGATGGGTTCGGAGAGAG+AGG - chr3.4:80656077-80656096 None:intergenic 60.0%
AACCCGGTCGGACCGGTCTA+AGG - chr3.4:80656140-80656159 None:intergenic 65.0%
ACCCGGTCGGACCGGTCTAA+GGG - chr3.4:80656139-80656158 None:intergenic 65.0%
CTCCCTTAGACCGGTCCGAC+CGG + chr3.4:80656134-80656153 MS.gene067085:CDS 65.0%
GGCGGCGGCGTTAAGTTTGG+AGG - chr3.4:80655918-80655937 None:intergenic 65.0%
GTTCCGGAGAACCGGGTGAC+AGG + chr3.4:80656157-80656176 MS.gene067085:CDS 65.0%
GTTTGACGGCGCGGAACCTG+AGG + chr3.4:80656294-80656313 MS.gene067085:CDS 65.0%
TCACCCGGTTCTCCGGAACC+CGG - chr3.4:80656156-80656175 None:intergenic 65.0%
TCCCTTAGACCGGTCCGACC+GGG + chr3.4:80656135-80656154 MS.gene067085:CDS 65.0%
AGACCGGTCCGACCGGGTTC+CGG + chr3.4:80656141-80656160 MS.gene067085:CDS 70.0%
CCGACCGGGTTCCGGAGAAC+CGG + chr3.4:80656149-80656168 MS.gene067085:CDS 70.0%
CCGGTTCTCCGGAACCCGGT+CGG - chr3.4:80656152-80656171 None:intergenic 70.0%
CGACCGGGTTCCGGAGAACC+GGG + chr3.4:80656150-80656169 MS.gene067085:CDS 70.0%
CGGTCGGACCGGTCTAAGGG+AGG - chr3.4:80656136-80656155 None:intergenic 70.0%
TCTCCGGAACCCGGTCGGAC+CGG - chr3.4:80656147-80656166 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 80655824 80656453 80655824 ID=MS.gene067085
chr3.4 mRNA 80655824 80656453 80655824 ID=MS.gene067085.t1;Parent=MS.gene067085
chr3.4 exon 80655824 80656453 80655824 ID=MS.gene067085.t1.exon1;Parent=MS.gene067085.t1
chr3.4 CDS 80655824 80656453 80655824 ID=cds.MS.gene067085.t1;Parent=MS.gene067085.t1
Gene Sequence

>MS.gene067085

ATGCTATATCCATTTCCATTTCCATTCCCAACTTCTACGGCAAGTTCCACCCTATTAACCCTCCCTCCTTCTCCTTCTCCTTCTCATTTCTCCTCCAAACTTAACGCCGCCGCCGTTACTTCAAAACAAATCCATCTCAAACTCTCCGTTAACCCTCCTCCCTTCAACAACACATTAACATCATCTTTATCATCATCACCGTCATCTCACGGTGTCACTGACAAGCTCAACAATCTCACCATCGAATTTGCCTCTCTCTCCGAACCCATCGACCGAGTAAAAAGACTATTACATTACGCATCTTTATTGCCTCCCTTAGACCGGTCCGACCGGGTTCCGGAGAACCGGGTGACAGGTTGTTCGACAGATGTTTGGGTGGTGGCCCACATAGACAAAGGTAGGAGAATGAGGTTTCAAGCAGACAGCGATTCGGAGATATCGAAGGGGTTCTGTTGGTGTCTTGTTTGGATGTTTGACGGCGCGGAACCTGAGGAAGTGCTTAACGTCGAGAAAGATGTTTTGGTTGGTATGAATGTAGTAGGGTTGAATAATGTTAAAGCACGTTCTAGGATTAACACGTGGCATAATATTTTCTTTGCCATGCAAAAGGCTACACAGGATTTAATTTAA

Protein sequence

>MS.gene067085.t1

MLYPFPFPFPTSTASSTLLTLPPSPSPSHFSSKLNAAAVTSKQIHLKLSVNPPPFNNTLTSSLSSSPSSHGVTDKLNNLTIEFASLSEPIDRVKRLLHYASLLPPLDRSDRVPENRVTGCSTDVWVVAHIDKGRRMRFQADSDSEISKGFCWCLVWMFDGAEPEEVLNVEKDVLVGMNVVGLNNVKARSRINTWHNIFFAMQKATQDLI