Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067814.t1 | XP_003624630.2 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 6.00E-18 | 99.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067814.t1 | Q9LVC0 | 54.1 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 7.0e-07 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067814.t1 | G7KZH1 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 4.4e-18 | 99.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene067814 | 0.805259 | 1.46E-49 | -1.69E-46 |
| MS.gene04915 | MS.gene067814 | 0.815097 | 1.10E-51 | -1.69E-46 |
| MS.gene04917 | MS.gene067814 | 0.817652 | 2.95E-52 | -1.69E-46 |
| MS.gene049887 | MS.gene067814 | 0.807454 | 5.02E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene067814 | 0.822514 | 2.27E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene067814 | 0.823657 | 1.23E-53 | -1.69E-46 |
| MS.gene050759 | MS.gene067814 | 0.805453 | 1.33E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene067814 | 0.837375 | 5.39E-57 | -1.69E-46 |
| MS.gene051003 | MS.gene067814 | 0.805106 | 1.57E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene067814 | 0.805101 | 1.57E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene067814 | 0.814574 | 1.44E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene067814 | 0.82115 | 4.71E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene067814 | 0.81006 | 1.39E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene067814 | 0.817466 | 3.25E-52 | -1.69E-46 |
| MS.gene053456 | MS.gene067814 | 0.819743 | 9.89E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene067814 | 0.806378 | 8.47E-50 | -1.69E-46 |
| MS.gene056480 | MS.gene067814 | 0.834634 | 2.68E-56 | -1.69E-46 |
| MS.gene059283 | MS.gene067814 | 0.809235 | 2.09E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene067814 | 0.809849 | 1.54E-50 | -1.69E-46 |
| MS.gene060296 | MS.gene067814 | 0.805484 | 1.31E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene067814 | 0.862484 | 4.91E-64 | -1.69E-46 |
| MS.gene060932 | MS.gene067814 | 0.860718 | 1.70E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067814.t1 | MTR_7g085780 | 98.305 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 2.48e-33 | 108 |
| MS.gene067814.t1 | MTR_6g029260 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 8.81e-15 | 61.6 |
| MS.gene067814.t1 | MTR_6g029330 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 1.94e-13 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067814.t1 | AT5G56540 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 2.43e-14 | 60.5 |
| MS.gene067814.t1 | AT3G13520 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 4.02e-11 | 52.4 |
Find 7 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCATAGCACTTGCATT+TGG | 0.360344 | 7.1:-19408109 | MS.gene067814:CDS |
| GGCAGTTATGGTTGCTATGT+TGG | 0.451707 | 7.1:-19408224 | MS.gene067814:CDS |
| GTAGCATCAGAAGCTGGACT+TGG | 0.472931 | 7.1:+19408160 | None:intergenic |
| TCAGAAGCTGGACTTGGAGC+TGG | 0.503706 | 7.1:+19408166 | None:intergenic |
| AGTGTTGTAGCATCAGAAGC+TGG | 0.542379 | 7.1:+19408154 | None:intergenic |
| ATGAGAGAAGCAAAAGCAGT+TGG | 0.550611 | 7.1:+19408124 | None:intergenic |
| ATGTTGGTGATAGCAACAAG+TGG | 0.753532 | 7.1:-19408208 | MS.gene067814:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAGATGAACATTTTTG+TGG | - | chr7.1:19408104-19408123 | MS.gene067814:CDS | 25.0% |
| ! | GAACATTTTTGTGGCAGTTA+TGG | - | chr7.1:19408113-19408132 | MS.gene067814:CDS | 35.0% |
| ATGAGAGAAGCAAAAGCAGT+TGG | + | chr7.1:19408228-19408247 | None:intergenic | 40.0% | |
| TCTCTCATAGCACTTGCATT+TGG | - | chr7.1:19408240-19408259 | MS.gene067814:CDS | 40.0% | |
| !! | ATGTTGGTGATAGCAACAAG+TGG | - | chr7.1:19408141-19408160 | MS.gene067814:CDS | 40.0% |
| AGTGTTGTAGCATCAGAAGC+TGG | + | chr7.1:19408198-19408217 | None:intergenic | 45.0% | |
| GGCAGTTATGGTTGCTATGT+TGG | - | chr7.1:19408125-19408144 | MS.gene067814:CDS | 45.0% | |
| GTAGCATCAGAAGCTGGACT+TGG | + | chr7.1:19408192-19408211 | None:intergenic | 50.0% | |
| TCAGAAGCTGGACTTGGAGC+TGG | + | chr7.1:19408186-19408205 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 19408096 | 19408275 | 19408096 | ID=MS.gene067814 |
| chr7.1 | mRNA | 19408096 | 19408275 | 19408096 | ID=MS.gene067814.t1;Parent=MS.gene067814 |
| chr7.1 | exon | 19408096 | 19408275 | 19408096 | ID=MS.gene067814.t1.exon1;Parent=MS.gene067814.t1 |
| chr7.1 | CDS | 19408096 | 19408275 | 19408096 | ID=cds.MS.gene067814.t1;Parent=MS.gene067814.t1 |
| Gene Sequence |
| Protein sequence |
