Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069571.t1 | XP_013458948.1 | 94.2 | 190 | 11 | 0 | 4 | 193 | 444 | 633 | 5.50E-68 | 267.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069571.t1 | A0A072V4A1 | 94.2 | 190 | 11 | 0 | 4 | 193 | 444 | 633 | 3.9e-68 | 267.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene069571 | -0.862496 | 4.87E-64 | -1.69E-46 |
| MS.gene04917 | MS.gene069571 | -0.856554 | 2.97E-62 | -1.69E-46 |
| MS.gene04991 | MS.gene069571 | 0.832457 | 9.35E-56 | -1.69E-46 |
| MS.gene050114 | MS.gene069571 | -0.817206 | 3.72E-52 | -1.69E-46 |
| MS.gene050629 | MS.gene069571 | 0.828147 | 1.06E-54 | -1.69E-46 |
| MS.gene050762 | MS.gene069571 | -0.858589 | 7.42E-63 | -1.69E-46 |
| MS.gene050763 | MS.gene069571 | -0.858714 | 6.81E-63 | -1.69E-46 |
| MS.gene051631 | MS.gene069571 | 0.829449 | 5.11E-55 | -1.69E-46 |
| MS.gene052242 | MS.gene069571 | 0.827621 | 1.41E-54 | -1.69E-46 |
| MS.gene054379 | MS.gene069571 | -0.847387 | 1.19E-59 | -1.69E-46 |
| MS.gene056752 | MS.gene069571 | -0.808764 | 2.64E-50 | -1.69E-46 |
| MS.gene056849 | MS.gene069571 | -0.821327 | 4.28E-53 | -1.69E-46 |
| MS.gene057451 | MS.gene069571 | 0.831471 | 1.64E-55 | -1.69E-46 |
| MS.gene057452 | MS.gene069571 | 0.858893 | 6.03E-63 | -1.69E-46 |
| MS.gene058278 | MS.gene069571 | -0.805686 | 1.18E-49 | -1.69E-46 |
| MS.gene058638 | MS.gene069571 | -0.819639 | 1.04E-52 | -1.69E-46 |
| MS.gene058639 | MS.gene069571 | -0.824045 | 9.98E-54 | -1.69E-46 |
| MS.gene058758 | MS.gene069571 | -0.815386 | 9.49E-52 | -1.69E-46 |
| MS.gene059719 | MS.gene069571 | -0.839544 | 1.49E-57 | -1.69E-46 |
| MS.gene059872 | MS.gene069571 | 0.803843 | 2.87E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene069571 | -0.813699 | 2.24E-51 | -1.69E-46 |
| MS.gene060563 | MS.gene069571 | -0.819549 | 1.10E-52 | -1.69E-46 |
| MS.gene060869 | MS.gene069571 | -0.845829 | 3.18E-59 | -1.69E-46 |
| MS.gene060870 | MS.gene069571 | -0.81901 | 1.45E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069571.t1 | MTR_3g012490 | 93.158 | 190 | 13 | 0 | 4 | 193 | 444 | 633 | 4.90e-116 | 343 |
| MS.gene069571.t1 | MTR_3g012500 | 47.651 | 149 | 67 | 5 | 53 | 193 | 518 | 663 | 3.51e-26 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 69 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATACCTTCAAGGTCTTATT+TGG | 0.158012 | 3.4:+9026696 | MS.gene069571:CDS |
| CTTTCATCTGTCCTATAATA+TGG | 0.177617 | 3.4:-9026460 | None:intergenic |
| CTACTCACAGGTGGCAAATA+TGG | 0.221515 | 3.4:-9026667 | None:intergenic |
| ACTTGGAGGCCCCTATTTAC+AGG | 0.258308 | 3.4:+9026509 | MS.gene069571:CDS |
| GGGGCATCGTTCCTGTAAAT+AGG | 0.310559 | 3.4:-9026520 | None:intergenic |
| ACTTGGACCACCCATATTAT+AGG | 0.347145 | 3.4:+9026449 | MS.gene069571:CDS |
| CTTCAAGGTCTTATTTGGTT+GGG | 0.353031 | 3.4:+9026701 | MS.gene069571:CDS |
| GGGCATCGTTCCTGTAAATA+GGG | 0.364497 | 3.4:-9026519 | None:intergenic |
| AGAAGCAGCATCCCGATAAT+AGG | 0.397822 | 3.4:-9026590 | None:intergenic |
| AAAGAGTAACGAGATGAAAC+TGG | 0.419260 | 3.4:-9026862 | None:intergenic |
| TCCATAGCGATAGCGATGAT+AGG | 0.421719 | 3.4:-9026629 | None:intergenic |
| CTACTTACAGAAAAGACAAA+AGG | 0.426240 | 3.4:+9026401 | MS.gene069571:CDS |
| CTCTCGTAAGGATCGGGTAT+AGG | 0.433579 | 3.4:-9026340 | None:intergenic |
| TAACCGAATGCAGCGTTACC+AGG | 0.436191 | 3.4:+9026813 | MS.gene069571:CDS |
| TTCAAGGTCTTATTTGGTTG+GGG | 0.445186 | 3.4:+9026702 | MS.gene069571:CDS |
| TTTCATCTGTCCTATAATAT+GGG | 0.448115 | 3.4:-9026459 | None:intergenic |
| CTCCCTGGTAACGCTGCATT+CGG | 0.450324 | 3.4:-9026816 | None:intergenic |
| CAAAGCATTAGCAGCAGAAT+GGG | 0.460591 | 3.4:-9026782 | None:intergenic |
| CCTTCAAGGTCTTATTTGGT+TGG | 0.461622 | 3.4:+9026700 | MS.gene069571:CDS |
| CACTCCCTCTCGTAAGGATC+GGG | 0.467045 | 3.4:-9026346 | None:intergenic |
| ATGAGCGTCAGACCTATTAT+CGG | 0.482684 | 3.4:+9026578 | MS.gene069571:CDS |
| AAGAGAGCGCCACCATAACT+TGG | 0.482756 | 3.4:+9026372 | MS.gene069571:CDS |
| GCGTTACCAGGGAGCAGAGT+TGG | 0.487889 | 3.4:+9026825 | MS.gene069571:CDS |
| CCAACCAAATAAGACCTTGA+AGG | 0.490029 | 3.4:-9026700 | None:intergenic |
| AAGTAGAGTGGTCCAAGTTA+TGG | 0.491907 | 3.4:-9026384 | None:intergenic |
| AAGAGAACGCCGCCATCACT+TGG | 0.498168 | 3.4:+9026432 | MS.gene069571:CDS |
| CCCTATCATCGCTATCGCTA+TGG | 0.508277 | 3.4:+9026628 | MS.gene069571:CDS |
| GTAGAGAGGAAATACCTTCA+AGG | 0.509053 | 3.4:+9026686 | MS.gene069571:CDS |
| GAGGATCAGCACCACGCGAA+GGG | 0.513475 | 3.4:-9026540 | None:intergenic |
| AAGAACTGATAACTTACAAA+GGG | 0.516990 | 3.4:+9026726 | MS.gene069571:CDS |
| ACAAAGCATTAGCAGCAGAA+TGG | 0.517512 | 3.4:-9026783 | None:intergenic |
| TAGTCTCCAACTCTGCTCCC+TGG | 0.517918 | 3.4:-9026831 | None:intergenic |
| TACTTACAGAAAAGACAAAA+GGG | 0.523510 | 3.4:+9026402 | MS.gene069571:CDS |
| CTTTCACACTCCCTCTCGTA+AGG | 0.527112 | 3.4:-9026352 | None:intergenic |
| ATTTAGCGAAGTGAATATGA+AGG | 0.529870 | 3.4:-9026892 | None:intergenic |
| AAGAGAGCGGCATCAACACT+TGG | 0.541372 | 3.4:+9026492 | MS.gene069571:CDS |
| CCATAGCGATAGCGATGATA+GGG | 0.545578 | 3.4:-9026628 | None:intergenic |
| CATATTATAGGACAGATGAA+AGG | 0.545719 | 3.4:+9026461 | MS.gene069571:CDS |
| ATATTATAGGACAGATGAAA+GGG | 0.547261 | 3.4:+9026462 | MS.gene069571:CDS |
| ACACTCCCTCTCGTAAGGAT+CGG | 0.551238 | 3.4:-9026347 | None:intergenic |
| GCGAAGTGAATATGAAGGAC+CGG | 0.558041 | 3.4:-9026887 | None:intergenic |
| TCGCTATGGAGCTTCTCCAC+GGG | 0.558939 | 3.4:+9026642 | MS.gene069571:CDS |
| ATCGCTATGGAGCTTCTCCA+CGG | 0.559316 | 3.4:+9026641 | MS.gene069571:CDS |
| TGAGCGTCAGACCTATTATC+GGG | 0.563638 | 3.4:+9026579 | MS.gene069571:CDS |
| AGAGGATCAGCACCACGCGA+AGG | 0.565202 | 3.4:-9026541 | None:intergenic |
| AATATGGGTGGTCCAAGTGA+TGG | 0.590652 | 3.4:-9026444 | None:intergenic |
| GGTATTTCCTCTCTACTCAC+AGG | 0.593234 | 3.4:-9026679 | None:intergenic |
| AGGAACGATGCCCCTTCGCG+TGG | 0.601263 | 3.4:+9026529 | MS.gene069571:CDS |
| CATCTGTCCTATAATATGGG+TGG | 0.603113 | 3.4:-9026456 | None:intergenic |
| AACCGAATGCAGCGTTACCA+GGG | 0.603445 | 3.4:+9026814 | MS.gene069571:CDS |
| AGGATCAGCACCACGCGAAG+GGG | 0.607172 | 3.4:-9026539 | None:intergenic |
| TCATCCTCGTTGAAACGAAG+AGG | 0.618964 | 3.4:-9026559 | None:intergenic |
| TCAAGGTCTTATTTGGTTGG+GGG | 0.628153 | 3.4:+9026703 | MS.gene069571:CDS |
| ATGGGTGGTCCAAGTGATGG+CGG | 0.641573 | 3.4:-9026441 | None:intergenic |
| GGCATCGTTCCTGTAAATAG+GGG | 0.642005 | 3.4:-9026518 | None:intergenic |
| GAGAGACTATTGCAGATGGT+AGG | 0.644689 | 3.4:+9026749 | MS.gene069571:CDS |
| GGTGGCAAATATGGATCCCG+TGG | 0.645028 | 3.4:-9026658 | None:intergenic |
| AGGGGAGAGACTATTGCAGA+TGG | 0.646135 | 3.4:+9026745 | MS.gene069571:CDS |
| TAGAGTGGTCCAAGTTATGG+TGG | 0.646648 | 3.4:-9026381 | None:intergenic |
| GAAGAACTGATAACTTACAA+AGG | 0.650124 | 3.4:+9026725 | MS.gene069571:CDS |
| TTTGCCACCTGTGAGTAGAG+AGG | 0.653420 | 3.4:+9026672 | MS.gene069571:CDS |
| AGAGCGGCATCAACACTTGG+AGG | 0.662295 | 3.4:+9026495 | MS.gene069571:CDS |
| TATACCCGATCCTTACGAGA+GGG | 0.664578 | 3.4:+9026342 | MS.gene069571:CDS |
| AAAGGGATTATGAAAGAGAG+CGG | 0.672814 | 3.4:+9026479 | MS.gene069571:CDS |
| ATTTCCTCTCTACTCACAGG+TGG | 0.682921 | 3.4:-9026676 | None:intergenic |
| CTATACCCGATCCTTACGAG+AGG | 0.693208 | 3.4:+9026341 | MS.gene069571:CDS |
| ATAGCGATGATAGGGATCGT+CGG | 0.696052 | 3.4:-9026620 | None:intergenic |
| TGATCCTCTTCGTTTCAACG+AGG | 0.704860 | 3.4:+9026555 | MS.gene069571:CDS |
| AGAACTGATAACTTACAAAG+GGG | 0.706264 | 3.4:+9026727 | MS.gene069571:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAACTGATAACTTACAAA+GGG | + | chr3.4:9026726-9026745 | MS.gene069571:CDS | 25.0% |
| ! | ATATTATAGGACAGATGAAA+GGG | + | chr3.4:9026462-9026481 | MS.gene069571:CDS | 25.0% |
| ! | TACTTACAGAAAAGACAAAA+GGG | + | chr3.4:9026402-9026421 | MS.gene069571:CDS | 25.0% |
| ! | TTTCATCTGTCCTATAATAT+GGG | - | chr3.4:9026462-9026481 | None:intergenic | 25.0% |
| AGAACTGATAACTTACAAAG+GGG | + | chr3.4:9026727-9026746 | MS.gene069571:CDS | 30.0% | |
| CATATTATAGGACAGATGAA+AGG | + | chr3.4:9026461-9026480 | MS.gene069571:CDS | 30.0% | |
| CTACTTACAGAAAAGACAAA+AGG | + | chr3.4:9026401-9026420 | MS.gene069571:CDS | 30.0% | |
| CTTTCATCTGTCCTATAATA+TGG | - | chr3.4:9026463-9026482 | None:intergenic | 30.0% | |
| GAAGAACTGATAACTTACAA+AGG | + | chr3.4:9026725-9026744 | MS.gene069571:CDS | 30.0% | |
| !! | AATACCTTCAAGGTCTTATT+TGG | + | chr3.4:9026696-9026715 | MS.gene069571:CDS | 30.0% |
| AAAGAGTAACGAGATGAAAC+TGG | - | chr3.4:9026865-9026884 | None:intergenic | 35.0% | |
| AAAGGGATTATGAAAGAGAG+CGG | + | chr3.4:9026479-9026498 | MS.gene069571:CDS | 35.0% | |
| ! | TGTCTTTTCTGTAAGTAGAG+TGG | - | chr3.4:9026399-9026418 | None:intergenic | 35.0% |
| !! | CTTCAAGGTCTTATTTGGTT+GGG | + | chr3.4:9026701-9026720 | MS.gene069571:CDS | 35.0% |
| !! | TTCAAGGTCTTATTTGGTTG+GGG | + | chr3.4:9026702-9026721 | MS.gene069571:CDS | 35.0% |
| AAGTAGAGTGGTCCAAGTTA+TGG | - | chr3.4:9026387-9026406 | None:intergenic | 40.0% | |
| ACTTGGACCACCCATATTAT+AGG | + | chr3.4:9026449-9026468 | MS.gene069571:CDS | 40.0% | |
| ATGAGCGTCAGACCTATTAT+CGG | + | chr3.4:9026578-9026597 | MS.gene069571:CDS | 40.0% | |
| CATCTGTCCTATAATATGGG+TGG | - | chr3.4:9026459-9026478 | None:intergenic | 40.0% | |
| CCAACCAAATAAGACCTTGA+AGG | - | chr3.4:9026703-9026722 | None:intergenic | 40.0% | |
| GTAGAGAGGAAATACCTTCA+AGG | + | chr3.4:9026686-9026705 | MS.gene069571:CDS | 40.0% | |
| ! | ACAAAGCATTAGCAGCAGAA+TGG | - | chr3.4:9026786-9026805 | None:intergenic | 40.0% |
| ! | CAAAGCATTAGCAGCAGAAT+GGG | - | chr3.4:9026785-9026804 | None:intergenic | 40.0% |
| ! | TCATCTCGTTACTCTTTTGC+CGG | + | chr3.4:9026868-9026887 | MS.gene069571:CDS | 40.0% |
| !! | CCTTCAAGGTCTTATTTGGT+TGG | + | chr3.4:9026700-9026719 | MS.gene069571:CDS | 40.0% |
| !! | TCAAGGTCTTATTTGGTTGG+GGG | + | chr3.4:9026703-9026722 | MS.gene069571:CDS | 40.0% |
| AATATGGGTGGTCCAAGTGA+TGG | - | chr3.4:9026447-9026466 | None:intergenic | 45.0% | |
| AGAAGCAGCATCCCGATAAT+AGG | - | chr3.4:9026593-9026612 | None:intergenic | 45.0% | |
| ATAGCGATGATAGGGATCGT+CGG | - | chr3.4:9026623-9026642 | None:intergenic | 45.0% | |
| ATTTCCTCTCTACTCACAGG+TGG | - | chr3.4:9026679-9026698 | None:intergenic | 45.0% | |
| CCATAGCGATAGCGATGATA+GGG | - | chr3.4:9026631-9026650 | None:intergenic | 45.0% | |
| CTACTCACAGGTGGCAAATA+TGG | - | chr3.4:9026670-9026689 | None:intergenic | 45.0% | |
| GAGAGACTATTGCAGATGGT+AGG | + | chr3.4:9026749-9026768 | MS.gene069571:CDS | 45.0% | |
| GCGAAGTGAATATGAAGGAC+CGG | - | chr3.4:9026890-9026909 | None:intergenic | 45.0% | |
| GGCATCGTTCCTGTAAATAG+GGG | - | chr3.4:9026521-9026540 | None:intergenic | 45.0% | |
| GGTATTTCCTCTCTACTCAC+AGG | - | chr3.4:9026682-9026701 | None:intergenic | 45.0% | |
| TAGAGTGGTCCAAGTTATGG+TGG | - | chr3.4:9026384-9026403 | None:intergenic | 45.0% | |
| TATACCCGATCCTTACGAGA+GGG | + | chr3.4:9026342-9026361 | MS.gene069571:CDS | 45.0% | |
| TCATCCTCGTTGAAACGAAG+AGG | - | chr3.4:9026562-9026581 | None:intergenic | 45.0% | |
| TCCATAGCGATAGCGATGAT+AGG | - | chr3.4:9026632-9026651 | None:intergenic | 45.0% | |
| TGAGCGTCAGACCTATTATC+GGG | + | chr3.4:9026579-9026598 | MS.gene069571:CDS | 45.0% | |
| TGATCCTCTTCGTTTCAACG+AGG | + | chr3.4:9026555-9026574 | MS.gene069571:CDS | 45.0% | |
| ! | GGGCATCGTTCCTGTAAATA+GGG | - | chr3.4:9026522-9026541 | None:intergenic | 45.0% |
| AACCGAATGCAGCGTTACCA+GGG | + | chr3.4:9026814-9026833 | MS.gene069571:CDS | 50.0% | |
| AAGAGAGCGGCATCAACACT+TGG | + | chr3.4:9026492-9026511 | MS.gene069571:CDS | 50.0% | |
| ACACTCCCTCTCGTAAGGAT+CGG | - | chr3.4:9026350-9026369 | None:intergenic | 50.0% | |
| ACTTGGAGGCCCCTATTTAC+AGG | + | chr3.4:9026509-9026528 | MS.gene069571:CDS | 50.0% | |
| AGGGGAGAGACTATTGCAGA+TGG | + | chr3.4:9026745-9026764 | MS.gene069571:CDS | 50.0% | |
| ATCGCTATGGAGCTTCTCCA+CGG | + | chr3.4:9026641-9026660 | MS.gene069571:CDS | 50.0% | |
| CCCTATCATCGCTATCGCTA+TGG | + | chr3.4:9026628-9026647 | MS.gene069571:CDS | 50.0% | |
| CTATACCCGATCCTTACGAG+AGG | + | chr3.4:9026341-9026360 | MS.gene069571:CDS | 50.0% | |
| CTCTCGTAAGGATCGGGTAT+AGG | - | chr3.4:9026343-9026362 | None:intergenic | 50.0% | |
| CTTTCACACTCCCTCTCGTA+AGG | - | chr3.4:9026355-9026374 | None:intergenic | 50.0% | |
| TAACCGAATGCAGCGTTACC+AGG | + | chr3.4:9026813-9026832 | MS.gene069571:CDS | 50.0% | |
| TTTGCCACCTGTGAGTAGAG+AGG | + | chr3.4:9026672-9026691 | MS.gene069571:CDS | 50.0% | |
| !! | AAGAGAGCGCCACCATAACT+TGG | + | chr3.4:9026372-9026391 | MS.gene069571:CDS | 50.0% |
| !! | GGGGCATCGTTCCTGTAAAT+AGG | - | chr3.4:9026523-9026542 | None:intergenic | 50.0% |
| AAGAGAACGCCGCCATCACT+TGG | + | chr3.4:9026432-9026451 | MS.gene069571:CDS | 55.0% | |
| AGAGCGGCATCAACACTTGG+AGG | + | chr3.4:9026495-9026514 | MS.gene069571:CDS | 55.0% | |
| ATGGGTGGTCCAAGTGATGG+CGG | - | chr3.4:9026444-9026463 | None:intergenic | 55.0% | |
| CACTCCCTCTCGTAAGGATC+GGG | - | chr3.4:9026349-9026368 | None:intergenic | 55.0% | |
| GGTGGCAAATATGGATCCCG+TGG | - | chr3.4:9026661-9026680 | None:intergenic | 55.0% | |
| TAGTCTCCAACTCTGCTCCC+TGG | - | chr3.4:9026834-9026853 | None:intergenic | 55.0% | |
| TCGCTATGGAGCTTCTCCAC+GGG | + | chr3.4:9026642-9026661 | MS.gene069571:CDS | 55.0% | |
| ! | CTCCCTGGTAACGCTGCATT+CGG | - | chr3.4:9026819-9026838 | None:intergenic | 55.0% |
| AGAGGATCAGCACCACGCGA+AGG | - | chr3.4:9026544-9026563 | None:intergenic | 60.0% | |
| AGGATCAGCACCACGCGAAG+GGG | - | chr3.4:9026542-9026561 | None:intergenic | 60.0% | |
| GAGGATCAGCACCACGCGAA+GGG | - | chr3.4:9026543-9026562 | None:intergenic | 60.0% | |
| GCGTTACCAGGGAGCAGAGT+TGG | + | chr3.4:9026825-9026844 | MS.gene069571:CDS | 60.0% | |
| AGGAACGATGCCCCTTCGCG+TGG | + | chr3.4:9026529-9026548 | MS.gene069571:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 9026331 | 9026912 | 9026331 | ID=MS.gene069571 |
| chr3.4 | mRNA | 9026331 | 9026912 | 9026331 | ID=MS.gene069571.t1;Parent=MS.gene069571 |
| chr3.4 | exon | 9026331 | 9026912 | 9026331 | ID=MS.gene069571.t1.exon1;Parent=MS.gene069571.t1 |
| chr3.4 | CDS | 9026331 | 9026912 | 9026331 | ID=cds.MS.gene069571.t1;Parent=MS.gene069571.t1 |
| Gene Sequence |
| Protein sequence |
