AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072366


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072366.t1 MTR_7g092570 95.816 239 9 1 1 239 1 238 1.20e-156 434
MS.gene072366.t1 MTR_7g007430 93.333 105 6 1 109 212 81 185 1.22e-56 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072366.t1 AT2G42260 48.333 180 81 6 64 239 88 259 1.68e-38 135
MS.gene072366.t1 AT2G42260 48.333 180 81 6 64 239 88 259 1.68e-38 135
MS.gene072366.t1 AT3G57860 40.625 256 122 10 1 239 1 243 2.19e-38 134

Find 62 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGCTTGATGCAGGATTT+TGG 0.284785 7.4:+16141795 None:intergenic
GCTTCATTCTCTGCTGCTAC+TGG 0.357062 7.4:-16141846 MS.gene072366:CDS
ATCAAGCTGAAAACACCTTA+TGG 0.387510 7.4:-16141783 MS.gene072366:CDS
ACGCGGCGGTCTCGTGCTTC+CGG 0.391950 7.4:+16143167 None:intergenic
TGAGAGAAGGAGGGAGAGAC+AGG 0.393153 7.4:-16141937 MS.gene072366:CDS
TTTGGAACTTTACTTCCATA+AGG 0.419855 7.4:+16141768 None:intergenic
GCTCGTGCGCGTCGTTCTCC+AGG 0.444790 7.4:-16142993 MS.gene072366:CDS
TTTAGGCGATTGAGAGAAGG+AGG 0.446441 7.4:-16141947 MS.gene072366:intron
TGCGCGTCGTTCTCCAGGTT+CGG 0.449151 7.4:-16142988 MS.gene072366:CDS
ATATCCTGCAACGGCGTCCT+CGG 0.451746 7.4:+16142885 None:intergenic
GTACCCGAGGACGCCGTTGC+AGG 0.452614 7.4:-16142889 MS.gene072366:CDS
CGATCTGAACCGGCAGTGAA+TGG 0.453141 7.4:+16143053 None:intergenic
ACTGAGGAAATTGAGCAAAC+TGG 0.455510 7.4:-16141912 MS.gene072366:CDS
ACGAGCAACTTCGCGACGTA+CGG 0.459911 7.4:+16143010 None:intergenic
GCAGTGAATGGAGCGGGTGC+AGG 0.465831 7.4:+16143065 None:intergenic
TTCACGCTCAATTATATCAA+TGG 0.466579 7.4:+16141662 None:intergenic
AGTGAAGAACCGATCTGAAC+CGG 0.469073 7.4:+16143043 None:intergenic
CCACGGCGAGTGGTGGTTGG+AGG 0.472234 7.4:+16142957 None:intergenic
TCTTGAATCACGGCGGAGAA+AGG 0.474672 7.4:+16143110 None:intergenic
ATCTTCTTCAATTCACCTCA+TGG 0.478558 7.4:+16141557 None:intergenic
GTGTTTAGGCGATTGAGAGA+AGG 0.495099 7.4:-16141950 MS.gene072366:intron
CAGAACATCAGAAAAGGGTT+AGG 0.496050 7.4:-16141590 MS.gene072366:CDS
CTTCCACGGCGAGTGGTGGT+TGG 0.501586 7.4:+16142954 None:intergenic
AAACTTCCTGCTGCTGAAGA+TGG 0.501739 7.4:-16141726 MS.gene072366:CDS
GATTCACCATCTTCAGCAGC+AGG 0.505435 7.4:+16141720 None:intergenic
TATCCTGCAACGGCGTCCTC+GGG 0.507871 7.4:+16142886 None:intergenic
TTCAGCTGGTTAGCATGAAC+TGG 0.509893 7.4:+16141885 None:intergenic
TCTTCTTCCACGGCGAGTGG+TGG 0.510955 7.4:+16142950 None:intergenic
GCACCCGCTCCATTCACTGC+CGG 0.525863 7.4:-16143062 MS.gene072366:CDS
GAACCGGCAGTGAATGGAGC+GGG 0.526734 7.4:+16143059 None:intergenic
AAAAGCAGAACATCAGAAAA+GGG 0.528951 7.4:-16141595 MS.gene072366:CDS
GTAGCAGCAGAGAATGAAGC+TGG 0.533970 7.4:+16141849 None:intergenic
GATCAGAGAAAATGTTCAGC+TGG 0.541232 7.4:+16141871 None:intergenic
CGAGCAACTTCGCGACGTAC+GGG 0.553693 7.4:+16143011 None:intergenic
CGAGGAGTGTACTTCCGTCT+TGG 0.554998 7.4:-16142911 MS.gene072366:CDS
ACCACTCGCCGTGGAAGAAG+AGG 0.555978 7.4:-16142948 MS.gene072366:CDS
GCCTCTTCTTCCACGGCGAG+TGG 0.556257 7.4:+16142947 None:intergenic
CATTCACTGCCGGTTCAGAT+CGG 0.557296 7.4:-16143052 MS.gene072366:CDS
AATTCACCTCATGGACATCA+AGG 0.566381 7.4:+16141566 None:intergenic
TTAGGCGATTGAGAGAAGGA+GGG 0.570115 7.4:-16141946 MS.gene072366:intron
AATAGAGTATCCACGTCAAG+AGG 0.577745 7.4:+16143140 None:intergenic
CCGCGTCATCCCTCTTGACG+TGG 0.577863 7.4:-16143150 MS.gene072366:CDS
TGGAGCGGGTGCAGGTGTAA+CGG 0.585593 7.4:+16143073 None:intergenic
GAATGCAATAACTTCTTCTG+AGG 0.598545 7.4:+16141684 None:intergenic
CGGCTCAGATTCTTGAATCA+CGG 0.600207 7.4:+16143100 None:intergenic
GAGGGAGAGACAGGGAACTG+AGG 0.604365 7.4:-16141928 MS.gene072366:CDS
CCTCCAACCACCACTCGCCG+TGG 0.618566 7.4:-16142957 MS.gene072366:CDS
GGCGTCCTCGGGTACCAAGA+CGG 0.620398 7.4:+16142897 None:intergenic
TACTTCCGTCTTGGTACCCG+AGG 0.623356 7.4:-16142902 MS.gene072366:CDS
GGTGCAGGTGTAACGGAGAG+CGG 0.629682 7.4:+16143080 None:intergenic
CACTCCTCGACGCAGTAGCA+CGG 0.633694 7.4:+16142925 None:intergenic
GAGAGAAGGAGGGAGAGACA+GGG 0.634717 7.4:-16141936 MS.gene072366:CDS
CCACGTCAAGAGGGATGACG+CGG 0.645758 7.4:+16143150 None:intergenic
ATAGAGTATCCACGTCAAGA+GGG 0.662352 7.4:+16143141 None:intergenic
TAGCACGGCCTCTTCTTCCA+CGG 0.680838 7.4:+16142940 None:intergenic
TCACGGCGGAGAAAGGTCGT+CGG 0.682516 7.4:+16143117 None:intergenic
ATAGCAGTGATATCCTGCAA+CGG 0.687557 7.4:+16142876 None:intergenic
TGAACCGGCAGTGAATGGAG+CGG 0.694026 7.4:+16143058 None:intergenic
GAGGCCGTGCTACTGCGTCG+AGG 0.702265 7.4:-16142929 MS.gene072366:CDS
CGTCAAGAGGGATGACGCGG+CGG 0.719322 7.4:+16143153 None:intergenic
TTAGGACCTTGATGTCCATG+AGG 0.720116 7.4:-16141572 MS.gene072366:CDS
CTCAGATTCTTGAATCACGG+CGG 0.734730 7.4:+16143103 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAATAAACTAAAAAGC+AGG + chr7.4:16142015-16142034 None:intergenic 15.0%
!!! AGAGAAAATTTTCATTATAA+CGG - chr7.4:16142267-16142286 MS.gene072366:intron 15.0%
!!! GAATTTGATTTAAATTGAAT+TGG - chr7.4:16142631-16142650 MS.gene072366:intron 15.0%
!!! TTGAATTTGATTTCATTTTT+CGG - chr7.4:16142087-16142106 MS.gene072366:intron 15.0%
!! ATTCTGTTAATATTGTGTTT+AGG - chr7.4:16142775-16142794 MS.gene072366:intron 20.0%
!! TCTCATCAAAATAGAAAAAA+CGG - chr7.4:16141921-16141940 MS.gene072366:CDS 20.0%
!! TTTAAATTGAATTGGCATAA+CGG - chr7.4:16142639-16142658 MS.gene072366:intron 20.0%
!!! TTGATTTGAATTGGAAAAAA+CGG - chr7.4:16142336-16142355 MS.gene072366:intron 20.0%
! CCGAATAAAGATAAATAAAC+CGG + chr7.4:16142148-16142167 None:intergenic 25.0%
! CCGGTTTATTTATCTTTATT+CGG - chr7.4:16142145-16142164 MS.gene072366:intron 25.0%
! CTTAATGCTTAATTGAAAAC+CGG - chr7.4:16142126-16142145 MS.gene072366:intron 25.0%
!! AGAGAAATTTTTCTTCAGAA+CGG - chr7.4:16142566-16142585 MS.gene072366:intron 25.0%
!! CTTTTACTTTGCTAATATTC+TGG - chr7.4:16142233-16142252 MS.gene072366:intron 25.0%
!! GGTTTTCAATTTTATCTAGA+AGG - chr7.4:16141942-16141961 MS.gene072366:CDS 25.0%
!!! GACTAGAATTTGATTTGAAT+TGG - chr7.4:16142327-16142346 MS.gene072366:intron 25.0%
AAAAAGCAGAACATCAGAAA+AGG - chr7.4:16143143-16143162 MS.gene072366:CDS 30.0%
AAAAGCAGAACATCAGAAAA+GGG - chr7.4:16143144-16143163 MS.gene072366:CDS 30.0%
TATATGCGATGCTGAATATT+CGG - chr7.4:16142031-16142050 MS.gene072366:intron 30.0%
TTCACGCTCAATTATATCAA+TGG + chr7.4:16143080-16143099 None:intergenic 30.0%
! GCAATGTCAATGATGATTTT+TGG + chr7.4:16142992-16143011 None:intergenic 30.0%
! TTTGGAACTTTACTTCCATA+AGG + chr7.4:16142974-16142993 None:intergenic 30.0%
!!! TTTCTGATGTTCTGCTTTTT+TGG + chr7.4:16143143-16143162 None:intergenic 30.0%
ATCAAGCTGAAAACACCTTA+TGG - chr7.4:16142956-16142975 MS.gene072366:CDS 35.0%
GAATGCAATAACTTCTTCTG+AGG + chr7.4:16143058-16143077 None:intergenic 35.0%
!! AGGATTTTGGTGATTTCTTG+TGG + chr7.4:16142934-16142953 None:intergenic 35.0%
ACTGAGGAAATTGAGCAAAC+TGG - chr7.4:16142827-16142846 MS.gene072366:intron 40.0%
ATAGCAGTGATATCCTGCAA+CGG + chr7.4:16141866-16141885 None:intergenic 40.0%
CAGAACATCAGAAAAGGGTT+AGG - chr7.4:16143149-16143168 MS.gene072366:CDS 40.0%
GATCAGAGAAAATGTTCAGC+TGG + chr7.4:16142871-16142890 None:intergenic 40.0%
! AATAGAGTATCCACGTCAAG+AGG + chr7.4:16141602-16141621 None:intergenic 40.0%
! ATAGAGTATCCACGTCAAGA+GGG + chr7.4:16141601-16141620 None:intergenic 40.0%
! TTCAGCTTGATGCAGGATTT+TGG + chr7.4:16142947-16142966 None:intergenic 40.0%
!!! TGTTCTGCTTTTTTGGCTGT+AGG + chr7.4:16143136-16143155 None:intergenic 40.0%
AAACTTCCTGCTGCTGAAGA+TGG - chr7.4:16143013-16143032 MS.gene072366:CDS 45.0%
AGTGAAGAACCGATCTGAAC+CGG + chr7.4:16141699-16141718 None:intergenic 45.0%
CGGCTCAGATTCTTGAATCA+CGG + chr7.4:16141642-16141661 None:intergenic 45.0%
CTCAGATTCTTGAATCACGG+CGG + chr7.4:16141639-16141658 None:intergenic 45.0%
GTGTTTAGGCGATTGAGAGA+AGG - chr7.4:16142789-16142808 MS.gene072366:intron 45.0%
TTAGGACCTTGATGTCCATG+AGG - chr7.4:16143167-16143186 MS.gene072366:CDS 45.0%
TTAGGCGATTGAGAGAAGGA+GGG - chr7.4:16142793-16142812 MS.gene072366:intron 45.0%
TTCAGCTGGTTAGCATGAAC+TGG + chr7.4:16142857-16142876 None:intergenic 45.0%
TTTAGGCGATTGAGAGAAGG+AGG - chr7.4:16142792-16142811 MS.gene072366:intron 45.0%
! AGGTGTTTTCAGCTTGATGC+AGG + chr7.4:16142954-16142973 None:intergenic 45.0%
GATTCACCATCTTCAGCAGC+AGG + chr7.4:16143022-16143041 None:intergenic 50.0%
GCTTCATTCTCTGCTGCTAC+TGG - chr7.4:16142893-16142912 MS.gene072366:CDS 50.0%
GTAGCAGCAGAGAATGAAGC+TGG + chr7.4:16142893-16142912 None:intergenic 50.0%
TCTTGAATCACGGCGGAGAA+AGG + chr7.4:16141632-16141651 None:intergenic 50.0%
!! CATTCACTGCCGGTTCAGAT+CGG - chr7.4:16141687-16141706 MS.gene072366:CDS 50.0%
!!! GGAGGAGTATTTTCCGAACC+TGG + chr7.4:16141767-16141786 None:intergenic 50.0%
ACGAGCAACTTCGCGACGTA+CGG + chr7.4:16141732-16141751 None:intergenic 55.0%
ATATCCTGCAACGGCGTCCT+CGG + chr7.4:16141857-16141876 None:intergenic 55.0%
CGATCTGAACCGGCAGTGAA+TGG + chr7.4:16141689-16141708 None:intergenic 55.0%
GAGAGAAGGAGGGAGAGACA+GGG - chr7.4:16142803-16142822 MS.gene072366:intron 55.0%
TACTTCCGTCTTGGTACCCG+AGG - chr7.4:16141837-16141856 MS.gene072366:CDS 55.0%
TAGCACGGCCTCTTCTTCCA+CGG + chr7.4:16141802-16141821 None:intergenic 55.0%
TGAACCGGCAGTGAATGGAG+CGG + chr7.4:16141684-16141703 None:intergenic 55.0%
TGAGAGAAGGAGGGAGAGAC+AGG - chr7.4:16142802-16142821 MS.gene072366:intron 55.0%
! CGAGGAGTGTACTTCCGTCT+TGG - chr7.4:16141828-16141847 MS.gene072366:CDS 55.0%
ACCACTCGCCGTGGAAGAAG+AGG - chr7.4:16141791-16141810 MS.gene072366:CDS 60.0%
CACTCCTCGACGCAGTAGCA+CGG + chr7.4:16141817-16141836 None:intergenic 60.0%
CCACGTCAAGAGGGATGACG+CGG + chr7.4:16141592-16141611 None:intergenic 60.0%
CGAGCAACTTCGCGACGTAC+GGG + chr7.4:16141731-16141750 None:intergenic 60.0%
GAACCGGCAGTGAATGGAGC+GGG + chr7.4:16141683-16141702 None:intergenic 60.0%
GAGGGAGAGACAGGGAACTG+AGG - chr7.4:16142811-16142830 MS.gene072366:intron 60.0%
TATCCTGCAACGGCGTCCTC+GGG + chr7.4:16141856-16141875 None:intergenic 60.0%
TCACGGCGGAGAAAGGTCGT+CGG + chr7.4:16141625-16141644 None:intergenic 60.0%
TCTTCTTCCACGGCGAGTGG+TGG + chr7.4:16141792-16141811 None:intergenic 60.0%
TGCGCGTCGTTCTCCAGGTT+CGG - chr7.4:16141751-16141770 MS.gene072366:CDS 60.0%
TGGAGCGGGTGCAGGTGTAA+CGG + chr7.4:16141669-16141688 None:intergenic 60.0%
! GGTGCAGGTGTAACGGAGAG+CGG + chr7.4:16141662-16141681 None:intergenic 60.0%
CCGCGTCATCCCTCTTGACG+TGG - chr7.4:16141589-16141608 MS.gene072366:CDS 65.0%
CGTCAAGAGGGATGACGCGG+CGG + chr7.4:16141589-16141608 None:intergenic 65.0%
CTTCCACGGCGAGTGGTGGT+TGG + chr7.4:16141788-16141807 None:intergenic 65.0%
GCACCCGCTCCATTCACTGC+CGG - chr7.4:16141677-16141696 MS.gene072366:CDS 65.0%
GCAGTGAATGGAGCGGGTGC+AGG + chr7.4:16141677-16141696 None:intergenic 65.0%
GCCTCTTCTTCCACGGCGAG+TGG + chr7.4:16141795-16141814 None:intergenic 65.0%
GGCGTCCTCGGGTACCAAGA+CGG + chr7.4:16141845-16141864 None:intergenic 65.0%
ACGCGGCGGTCTCGTGCTTC+CGG + chr7.4:16141575-16141594 None:intergenic 70.0%
CCACGGCGAGTGGTGGTTGG+AGG + chr7.4:16141785-16141804 None:intergenic 70.0%
CCTCCAACCACCACTCGCCG+TGG - chr7.4:16141782-16141801 MS.gene072366:CDS 70.0%
GCTCGTGCGCGTCGTTCTCC+AGG - chr7.4:16141746-16141765 MS.gene072366:CDS 70.0%
GTACCCGAGGACGCCGTTGC+AGG - chr7.4:16141850-16141869 MS.gene072366:CDS 70.0%
! GAGGCCGTGCTACTGCGTCG+AGG - chr7.4:16141810-16141829 MS.gene072366:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 16141569 16143192 16141569 ID=MS.gene072366
chr7.4 mRNA 16141569 16143192 16141569 ID=MS.gene072366.t1;Parent=MS.gene072366
chr7.4 exon 16142869 16143192 16142869 ID=MS.gene072366.t1.exon1;Parent=MS.gene072366.t1
chr7.4 CDS 16142869 16143192 16142869 ID=cds.MS.gene072366.t1;Parent=MS.gene072366.t1
chr7.4 exon 16141569 16141964 16141569 ID=MS.gene072366.t1.exon2;Parent=MS.gene072366.t1
chr7.4 CDS 16141569 16141964 16141569 ID=cds.MS.gene072366.t1;Parent=MS.gene072366.t1
Gene Sequence

>MS.gene072366

ATGCCGGAAGCACGAGACCGCCGCGTCATCCCTCTTGACGTGGATACTCTATTCCGACGACCTTTCTCCGCCGTGATTCAAGAATCTGAGCCGCTCTCCGTTACACCTGCACCCGCTCCATTCACTGCCGGTTCAGATCGGTTCTTCACTGAAAGAACTCCCGTACGTCGCGAAGTTGCTCGTGCGCGTCGTTCTCCAGGTTCGGAAAATACTCCTCCAACCACCACTCGCCGTGGAAGAAGAGGCCGTGCTACTGCGTCGAGGAGTGTACTTCCGTCTTGGTACCCGAGGACGCCGTTGCAGGATATCACTGCTATTGTTCGAGTAAATCTCTGATTCTTTTCTCTTTCTCTCTCATCAAAATAGAAAAAACGGTTTTCAATTTTATCTAGAAGGTTCTTTGAATATTTTTAAATTTTAGTTTAATATTAATATAATGATATCCTGCTTTTTAGTTTATTTTATATGCGATGCTGAATATTCGGAATGCTTAGTTTACTTTGATGTAGAACTTGAATTTGAATTTGATTTCATTTTTCGGTGTTTGTTTATTGATGCTTAATGCTTAATTGAAAACCGGTTTATTTATCTTTATTCGGTTGTTTTGATAATTTGTTATGTGATGCTTAATTGTCTGTGATGAGTTTATCTTTAATTTGATTTGCTTTTACTTTGCTAATATTCTGGTTAGCAAATTGAGAGAAAATTTTCATTATAACGGTTTAGCTTTAATCTTAAACGTTCTTTTGAGATACTTTGACTAGAATTTGATTTGAATTGGAAAAAACGGTTAATCTTTAATTAATCTAGAACGTTCTTTTGTTTTTAATATATATTTTGAATTTGAATTTTGAACTTGATTTTAGTTTAATTATATTAAATTCTGTCACAATATTCTGTTGTTCATTTGTTATGTGATACTTAATTTTCTGTGTTTAGTTTACGTTTAATTTGAATTTGATTTGATTTTACTTTGTTAATACTGTTTATTGAATTGAGAGAAATTTTTCTTCAGAACGGTTTAACTTTAAGCATAACTTCTCTTTGAAGTGTATTTGACTAGAATTTGATTTAAATTGAATTGGCATAACGGCTGTTTGTTGAGTTCAATTTGAATTGAATATGAACTTTATCGTTGTTAATGTTCTGTTGATTTGTTATGTGATGATGAATTTTCTGTATGTTTAGTTCAATCTGACTAATTTGATTCTGTTAATATTGTGTTTAGGCGATTGAGAGAAGGAGGGAGAGACAGGGAACTGAGGAAATTGAGCAAACTGGAACACCAGTTCATGCTAACCAGCTGAACATTTTCTCTGATCCAGCTTCATTCTCTGCTGCTACTGGATCTTCTTCACGTATCCACAAGAAATCACCAAAATCCTGCATCAAGCTGAAAACACCTTATGGAAGTAAAGTTCCAAAAATCATCATTGACATTGCAAAACTTCCTGCTGCTGAAGATGGTGAATCAGAGCTTCTGACTCCTCAGAAGAAGTTATTGCATTCCATTGATATAATTGAGCGTGAAGTGAAGCAAGAGTTGATGAAACTCAAGAGAACTCCTACAGCCAAAAAAGCAGAACATCAGAAAAGGGTTAGGACCTTGATGTCCATGAGGTGA

Protein sequence

>MS.gene072366.t1

MPEARDRRVIPLDVDTLFRRPFSAVIQESEPLSVTPAPAPFTAGSDRFFTERTPVRREVARARRSPGSENTPPTTTRRGRRGRATASRSVLPSWYPRTPLQDITAIVRAIERRRERQGTEEIEQTGTPVHANQLNIFSDPASFSAATGSSSRIHKKSPKSCIKLKTPYGSKVPKIIIDIAKLPAAEDGESELLTPQKKLLHSIDIIEREVKQELMKLKRTPTAKKAEHQKRVRTLMSMR