Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07297.t1 | XP_003591392.1 | 98.2 | 114 | 2 | 0 | 1 | 114 | 191 | 304 | 5.80E-57 | 229.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07297.t1 | Q9S834 | 96.2 | 105 | 4 | 0 | 1 | 105 | 194 | 298 | 9.6e-53 | 207.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07297.t1 | G7ICX2 | 98.2 | 114 | 2 | 0 | 1 | 114 | 191 | 304 | 4.2e-57 | 229.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene07297 | 0.810477 | 1.13E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene07297 | 0.809924 | 1.49E-50 | -1.69E-46 |
| MS.gene050747 | MS.gene07297 | 0.801977 | 6.98E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene07297 | 0.800867 | 1.18E-48 | -1.69E-46 |
| MS.gene050812 | MS.gene07297 | 0.833146 | 6.31E-56 | -1.69E-46 |
| MS.gene05081 | MS.gene07297 | 0.842692 | 2.21E-58 | -1.69E-46 |
| MS.gene051525 | MS.gene07297 | 0.806715 | 7.19E-50 | -1.69E-46 |
| MS.gene051552 | MS.gene07297 | -0.814161 | 1.77E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene07297 | 0.806838 | 6.77E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene07297 | 0.828903 | 6.94E-55 | -1.69E-46 |
| MS.gene052469 | MS.gene07297 | 0.858262 | 9.30E-63 | -1.69E-46 |
| MS.gene052870 | MS.gene07297 | 0.812926 | 3.32E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene07297 | 0.813459 | 2.53E-51 | -1.69E-46 |
| MS.gene052982 | MS.gene07297 | 0.803848 | 2.87E-49 | -1.69E-46 |
| MS.gene053579 | MS.gene07297 | 0.811205 | 7.87E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene07297 | 0.819152 | 1.35E-52 | -1.69E-46 |
| MS.gene054107 | MS.gene07297 | 0.810404 | 1.17E-50 | -1.69E-46 |
| MS.gene054408 | MS.gene07297 | 0.805146 | 1.54E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene07297 | 0.829422 | 5.19E-55 | -1.69E-46 |
| MS.gene055206 | MS.gene07297 | 0.805154 | 1.53E-49 | -1.69E-46 |
| MS.gene055224 | MS.gene07297 | 0.808967 | 2.39E-50 | -1.69E-46 |
| MS.gene055539 | MS.gene07297 | 0.844082 | 9.42E-59 | -1.69E-46 |
| MS.gene055577 | MS.gene07297 | 0.804046 | 2.61E-49 | -1.69E-46 |
| MS.gene055741 | MS.gene07297 | 0.83298 | 6.94E-56 | -1.69E-46 |
| MS.gene055744 | MS.gene07297 | 0.834956 | 2.22E-56 | -1.69E-46 |
| MS.gene055941 | MS.gene07297 | 0.803819 | 2.91E-49 | -1.69E-46 |
| MS.gene056659 | MS.gene07297 | 0.808726 | 2.69E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene07297 | 0.813867 | 2.06E-51 | -1.69E-46 |
| MS.gene056903 | MS.gene07297 | 0.830832 | 2.35E-55 | -1.69E-46 |
| MS.gene057124 | MS.gene07297 | 0.81602 | 6.86E-52 | -1.69E-46 |
| MS.gene057332 | MS.gene07297 | 0.808278 | 3.35E-50 | -1.69E-46 |
| MS.gene058158 | MS.gene07297 | -0.829353 | 5.40E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene07297 | 0.824004 | 1.02E-53 | -1.69E-46 |
| MS.gene058418 | MS.gene07297 | -0.804438 | 2.16E-49 | -1.69E-46 |
| MS.gene058444 | MS.gene07297 | 0.816166 | 6.36E-52 | -1.69E-46 |
| MS.gene058700 | MS.gene07297 | 0.821278 | 4.40E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene07297 | 0.820357 | 7.16E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene07297 | 0.821808 | 3.32E-53 | -1.69E-46 |
| MS.gene05914 | MS.gene07297 | 0.809896 | 1.51E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene07297 | 0.824874 | 6.37E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene07297 | 0.827521 | 1.49E-54 | -1.69E-46 |
| MS.gene061231 | MS.gene07297 | 0.838743 | 2.40E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGATGAGTCCATATTCTT+TGG | 0.288327 | 1.4:-69310421 | None:intergenic |
| GCAAAAGAAGCCAAAGAATA+TGG | 0.292792 | 1.4:+69310411 | MS.gene07297:CDS |
| GATAGGACAGATACCCATTC+AGG | 0.309008 | 1.4:-69310329 | None:intergenic |
| TGAATCATTATCCTTGAGTT+TGG | 0.321613 | 1.4:-69309819 | None:intergenic |
| GCAAACTGACATAGATATTC+AGG | 0.327655 | 1.4:+69309869 | MS.gene07297:CDS |
| TAAACTTTGCCCAGTTTGAT+AGG | 0.348450 | 1.4:-69310346 | None:intergenic |
| GGGTATCTGTCCTATCAAAC+TGG | 0.363774 | 1.4:+69310336 | MS.gene07297:CDS |
| GGAATCATTACCTTTGGTTC+CGG | 0.378564 | 1.4:-69309670 | None:intergenic |
| TCTCAAAGCTCTTCAGCCTT+TGG | 0.393350 | 1.4:+69310461 | MS.gene07297:CDS |
| CCCTCTTCTGCAGCTGCCAA+AGG | 0.448770 | 1.4:-69310477 | None:intergenic |
| CATCACAAGGCAAACCTGAA+TGG | 0.459835 | 1.4:+69310315 | MS.gene07297:CDS |
| ATCACAAGGCAAACCTGAAT+GGG | 0.495914 | 1.4:+69310316 | MS.gene07297:CDS |
| CTGCTTAGTGCCGGAACCAA+AGG | 0.500748 | 1.4:+69309660 | MS.gene07297:CDS |
| GCCTTTGGCAGCTGCAGAAG+AGG | 0.529560 | 1.4:+69310476 | MS.gene07297:CDS |
| ATGATTCATCAACCGCTTGG+TGG | 0.536787 | 1.4:+69309834 | MS.gene07297:CDS |
| GTTTCAGCTTGCCAAACTCA+AGG | 0.562494 | 1.4:+69309808 | MS.gene07297:CDS |
| CTGCAGAAGAGGGTAAAGAC+CGG | 0.577540 | 1.4:+69310487 | MS.gene07297:CDS |
| AAATGAAATGCTGCATCACA+AGG | 0.584369 | 1.4:+69310302 | MS.gene07297:CDS |
| CCTTTGGCAGCTGCAGAAGA+GGG | 0.585082 | 1.4:+69310477 | MS.gene07297:CDS |
| AAGTTTAGAGAAAATTAACC+AGG | 0.591158 | 1.4:+69310362 | MS.gene07297:CDS |
| GAAATTAGGAAATAATAGCC+CGG | 0.595521 | 1.4:-69310506 | None:intergenic |
| ATAATGATTCATCAACCGCT+TGG | 0.597203 | 1.4:+69309831 | MS.gene07297:CDS |
| TGCAGAAGAGGGTAAAGACC+GGG | 0.605444 | 1.4:+69310488 | MS.gene07297:CDS |
| GGTATCTGTCCTATCAAACT+GGG | 0.622810 | 1.4:+69310337 | MS.gene07297:CDS |
| AAAGAATATGGACTCATCGA+TGG | 0.623110 | 1.4:+69310423 | MS.gene07297:CDS |
| AAAGGCTGAAGAGCTTTGAG+AGG | 0.630190 | 1.4:-69310459 | None:intergenic |
| CCTCCTTGAAAACCACCAAG+CGG | 0.689995 | 1.4:-69309846 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATATTATCATGGTTTA+GGG | - | chr1.4:69310028-69310047 | None:intergenic | 15.0% |
| !!! | TCTTTTTCATAGAAAATTAA+AGG | + | chr1.4:69309967-69309986 | MS.gene07297:intron | 15.0% |
| !! | AAAATAGGAATATAAGAGTA+TGG | - | chr1.4:69310068-69310087 | None:intergenic | 20.0% |
| !! | AATAAAAATAGAACAGGTAT+AGG | - | chr1.4:69310097-69310116 | None:intergenic | 20.0% |
| !! | ATAGATATTCAGGTATATAT+AGG | + | chr1.4:69309879-69309898 | MS.gene07297:intron | 20.0% |
| !! | GTTTAATATTATCATGGTTT+AGG | - | chr1.4:69310029-69310048 | None:intergenic | 20.0% |
| !!! | ATCCATTTTTCTTCTTTTTT+AGG | + | chr1.4:69309712-69309731 | MS.gene07297:intron | 20.0% |
| !!! | ATTTTATCAAAAGAAACAAG+AGG | - | chr1.4:69309941-69309960 | None:intergenic | 20.0% |
| ! | AAGTTTAGAGAAAATTAACC+AGG | + | chr1.4:69310362-69310381 | MS.gene07297:CDS | 25.0% |
| ! | GAATGGAATAAAAATAGAAC+AGG | - | chr1.4:69310103-69310122 | None:intergenic | 25.0% |
| ! | GACCTAAAAAAGAAGAAAAA+TGG | - | chr1.4:69309717-69309736 | None:intergenic | 25.0% |
| ! | TTTGAACACCTATACAATAA+GGG | + | chr1.4:69310176-69310195 | MS.gene07297:intron | 25.0% |
| !! | TTTTGAACACCTATACAATA+AGG | + | chr1.4:69310175-69310194 | MS.gene07297:intron | 25.0% |
| AAATAGAACAGGTATAGGAA+AGG | - | chr1.4:69310092-69310111 | None:intergenic | 30.0% | |
| AGAAAATTAAAGGCTATCAC+AGG | + | chr1.4:69309977-69309996 | MS.gene07297:intron | 30.0% | |
| AGAGTATGGAACAATATTGT+GGG | - | chr1.4:69310054-69310073 | None:intergenic | 30.0% | |
| AGGTATAGGAAAGGAAAAAT+AGG | - | chr1.4:69310083-69310102 | None:intergenic | 30.0% | |
| ATACAGTCATTGCAAGTTAA+AGG | - | chr1.4:69309694-69309713 | None:intergenic | 30.0% | |
| GATGATATCCCTTATTGTAT+AGG | - | chr1.4:69310187-69310206 | None:intergenic | 30.0% | |
| GTTAAAGGAATCATTACCTT+TGG | - | chr1.4:69309679-69309698 | None:intergenic | 30.0% | |
| TGAATCATTATCCTTGAGTT+TGG | - | chr1.4:69309822-69309841 | None:intergenic | 30.0% | |
| TGGGGAGTTTAATATTATCA+TGG | - | chr1.4:69310035-69310054 | None:intergenic | 30.0% | |
| TTATGATTCATAATCCTCTC+AGG | + | chr1.4:69310278-69310297 | MS.gene07297:intron | 30.0% | |
| TTGCACTCATGAAAAAATCA+CGG | - | chr1.4:69310395-69310414 | None:intergenic | 30.0% | |
| ! | AAGAGTATGGAACAATATTG+TGG | - | chr1.4:69310055-69310074 | None:intergenic | 30.0% |
| ! | ATTTTTCCCTTTTGCAAAAG+TGG | - | chr1.4:69309739-69309758 | None:intergenic | 30.0% |
| !!! | TTTTGGCTTTAGTTGGTTTT+TGG | + | chr1.4:69310133-69310152 | MS.gene07297:intron | 30.0% |
| AAAGAATATGGACTCATCGA+TGG | + | chr1.4:69310423-69310442 | MS.gene07297:CDS | 35.0% | |
| AAATGAAATGCTGCATCACA+AGG | + | chr1.4:69310302-69310321 | MS.gene07297:CDS | 35.0% | |
| ATAATGATTCATCAACCGCT+TGG | + | chr1.4:69309831-69309850 | MS.gene07297:CDS | 35.0% | |
| ATCGATGAGTCCATATTCTT+TGG | - | chr1.4:69310424-69310443 | None:intergenic | 35.0% | |
| ATGGTTTAGGGATAACTCAT+TGG | - | chr1.4:69310016-69310035 | None:intergenic | 35.0% | |
| GAGTATGGAACAATATTGTG+GGG | - | chr1.4:69310053-69310072 | None:intergenic | 35.0% | |
| GCAAAAGAAGCCAAAGAATA+TGG | + | chr1.4:69310411-69310430 | MS.gene07297:CDS | 35.0% | |
| GCAAACTGACATAGATATTC+AGG | + | chr1.4:69309869-69309888 | MS.gene07297:CDS | 35.0% | |
| TAAACTTTGCCCAGTTTGAT+AGG | - | chr1.4:69310349-69310368 | None:intergenic | 35.0% | |
| TCCTTCAAAAATGACAGAGT+CGG | - | chr1.4:69309784-69309803 | None:intergenic | 35.0% | |
| ! | CTAAAGCCAAAAGTATGACA+TGG | - | chr1.4:69310125-69310144 | None:intergenic | 35.0% |
| ! | TCCATTCCATGTCATACTTT+TGG | + | chr1.4:69310116-69310135 | MS.gene07297:intron | 35.0% |
| !! | TTAGGTCCACTTTTGCAAAA+GGG | + | chr1.4:69309730-69309749 | MS.gene07297:intron | 35.0% |
| !! | TTTAGGTCCACTTTTGCAAA+AGG | + | chr1.4:69309729-69309748 | MS.gene07297:intron | 35.0% |
| !!! | GCTTTAGTTGGTTTTTGGAT+TGG | + | chr1.4:69310138-69310157 | MS.gene07297:intron | 35.0% |
| !!! | GTCATACTTTTGGCTTTAGT+TGG | + | chr1.4:69310126-69310145 | MS.gene07297:intron | 35.0% |
| AAAAATCACGGTCTGTATCC+TGG | - | chr1.4:69310383-69310402 | None:intergenic | 40.0% | |
| AGGTTTGTGCCATCAAATGA+GGG | - | chr1.4:69309921-69309940 | None:intergenic | 40.0% | |
| ATCACAAGGCAAACCTGAAT+GGG | + | chr1.4:69310316-69310335 | MS.gene07297:CDS | 40.0% | |
| CCTTCAAAAATGACAGAGTC+GGG | - | chr1.4:69309783-69309802 | None:intergenic | 40.0% | |
| CTAACCAATCATGTCTTCCT+TGG | - | chr1.4:69310216-69310235 | None:intergenic | 40.0% | |
| GGTATCTGTCCTATCAAACT+GGG | + | chr1.4:69310337-69310356 | MS.gene07297:CDS | 40.0% | |
| ! | CAAGGAAGACATGATTGGTT+AGG | + | chr1.4:69310214-69310233 | MS.gene07297:intron | 40.0% |
| ! | GCCAAAAGTATGACATGGAA+TGG | - | chr1.4:69310120-69310139 | None:intergenic | 40.0% |
| ! | GGAATCATTACCTTTGGTTC+CGG | - | chr1.4:69309673-69309692 | None:intergenic | 40.0% |
| ATCATGTCTTCCTTGGCACA+TGG | - | chr1.4:69310209-69310228 | None:intergenic | 45.0% | |
| ATGATTCATCAACCGCTTGG+TGG | + | chr1.4:69309834-69309853 | MS.gene07297:CDS | 45.0% | |
| CAGCATTTCATTTGCCTGAG+AGG | - | chr1.4:69310295-69310314 | None:intergenic | 45.0% | |
| CATCACAAGGCAAACCTGAA+TGG | + | chr1.4:69310315-69310334 | MS.gene07297:CDS | 45.0% | |
| GAGGTTTGTGCCATCAAATG+AGG | - | chr1.4:69309922-69309941 | None:intergenic | 45.0% | |
| GATAGGACAGATACCCATTC+AGG | - | chr1.4:69310332-69310351 | None:intergenic | 45.0% | |
| GGGTATCTGTCCTATCAAAC+TGG | + | chr1.4:69310336-69310355 | MS.gene07297:CDS | 45.0% | |
| GGTTTGTGCCATCAAATGAG+GGG | - | chr1.4:69309920-69309939 | None:intergenic | 45.0% | |
| GTTTCAGCTTGCCAAACTCA+AGG | + | chr1.4:69309808-69309827 | MS.gene07297:CDS | 45.0% | |
| GTTTGTGCCATCAAATGAGG+GGG | - | chr1.4:69309919-69309938 | None:intergenic | 45.0% | |
| TCTCAAAGCTCTTCAGCCTT+TGG | + | chr1.4:69310461-69310480 | MS.gene07297:CDS | 45.0% | |
| TGTGCCAAGGAAGACATGAT+TGG | + | chr1.4:69310209-69310228 | MS.gene07297:intron | 45.0% | |
| TTTGTGCCATCAAATGAGGG+GGG | - | chr1.4:69309918-69309937 | None:intergenic | 45.0% | |
| !! | AAAGGCTGAAGAGCTTTGAG+AGG | - | chr1.4:69310462-69310481 | None:intergenic | 45.0% |
| !!! | CCCGACTCTGTCATTTTTGA+AGG | + | chr1.4:69309780-69309799 | MS.gene07297:intron | 45.0% |
| AATGAGGGGGGGAAGAAAAC+AGG | - | chr1.4:69309906-69309925 | None:intergenic | 50.0% | |
| ATGAGGGGGGGAAGAAAACA+GGG | - | chr1.4:69309905-69309924 | None:intergenic | 50.0% | |
| CCTCCTTGAAAACCACCAAG+CGG | - | chr1.4:69309849-69309868 | None:intergenic | 50.0% | |
| CTGCAGAAGAGGGTAAAGAC+CGG | + | chr1.4:69310487-69310506 | MS.gene07297:CDS | 50.0% | |
| GGGATATCATCCATGTGCCA+AGG | + | chr1.4:69310196-69310215 | MS.gene07297:intron | 50.0% | |
| TGCAGAAGAGGGTAAAGACC+GGG | + | chr1.4:69310488-69310507 | MS.gene07297:CDS | 50.0% | |
| TTCTTCCCCCCCTCATTTGA+TGG | + | chr1.4:69309909-69309928 | MS.gene07297:intron | 50.0% | |
| TTGTGCCATCAAATGAGGGG+GGG | - | chr1.4:69309917-69309936 | None:intergenic | 50.0% | |
| ! | GGAGCTTTTCTGCTTAGTGC+CGG | + | chr1.4:69309651-69309670 | MS.gene07297:CDS | 50.0% |
| !! | CAACCGCTTGGTGGTTTTCA+AGG | + | chr1.4:69309843-69309862 | MS.gene07297:CDS | 50.0% |
| !! | CGCTTGGTGGTTTTCAAGGA+GGG | + | chr1.4:69309847-69309866 | MS.gene07297:CDS | 50.0% |
| CCTTTGGCAGCTGCAGAAGA+GGG | + | chr1.4:69310477-69310496 | MS.gene07297:CDS | 55.0% | |
| ! | CTGCTTAGTGCCGGAACCAA+AGG | + | chr1.4:69309660-69309679 | MS.gene07297:CDS | 55.0% |
| !! | CCGCTTGGTGGTTTTCAAGG+AGG | + | chr1.4:69309846-69309865 | MS.gene07297:CDS | 55.0% |
| CCCTCTTCTGCAGCTGCCAA+AGG | - | chr1.4:69310480-69310499 | None:intergenic | 60.0% | |
| GCCTTTGGCAGCTGCAGAAG+AGG | + | chr1.4:69310476-69310495 | MS.gene07297:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 69309647 | 69310524 | 69309647 | ID=MS.gene07297 |
| chr1.4 | mRNA | 69309647 | 69310524 | 69309647 | ID=MS.gene07297.t1;Parent=MS.gene07297 |
| chr1.4 | exon | 69309647 | 69309681 | 69309647 | ID=MS.gene07297.t1.exon1;Parent=MS.gene07297.t1 |
| chr1.4 | CDS | 69309647 | 69309681 | 69309647 | ID=cds.MS.gene07297.t1;Parent=MS.gene07297.t1 |
| chr1.4 | exon | 69309802 | 69309890 | 69309802 | ID=MS.gene07297.t1.exon2;Parent=MS.gene07297.t1 |
| chr1.4 | CDS | 69309802 | 69309890 | 69309802 | ID=cds.MS.gene07297.t1;Parent=MS.gene07297.t1 |
| chr1.4 | exon | 69310300 | 69310524 | 69310300 | ID=MS.gene07297.t1.exon3;Parent=MS.gene07297.t1 |
| chr1.4 | CDS | 69310300 | 69310524 | 69310300 | ID=cds.MS.gene07297.t1;Parent=MS.gene07297.t1 |
| Gene Sequence |
| Protein sequence |
