Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08887.t1 | XP_013446515.1 | 100 | 123 | 0 | 0 | 1 | 123 | 157 | 279 | 6.20E-60 | 240 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08887.t1 | Q54EP7 | 36.1 | 119 | 75 | 1 | 2 | 120 | 153 | 270 | 1.2e-14 | 80.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08887.t1 | A0A072TSZ4 | 100.0 | 123 | 0 | 0 | 1 | 123 | 157 | 279 | 4.5e-60 | 240.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049887 | MS.gene08887 | 0.821538 | 3.83E-53 | -1.69E-46 |
| MS.gene050084 | MS.gene08887 | 0.831916 | 1.27E-55 | -1.69E-46 |
| MS.gene050085 | MS.gene08887 | 0.838453 | 2.85E-57 | -1.69E-46 |
| MS.gene050224 | MS.gene08887 | 0.830111 | 3.53E-55 | -1.69E-46 |
| MS.gene050359 | MS.gene08887 | 0.839408 | 1.61E-57 | -1.69E-46 |
| MS.gene050551 | MS.gene08887 | 0.831795 | 1.36E-55 | -1.69E-46 |
| MS.gene050838 | MS.gene08887 | 0.849128 | 3.95E-60 | -1.69E-46 |
| MS.gene051102 | MS.gene08887 | 0.817683 | 2.90E-52 | -1.69E-46 |
| MS.gene051234 | MS.gene08887 | 0.810922 | 9.06E-51 | -1.69E-46 |
| MS.gene051343 | MS.gene08887 | 0.80026 | 1.57E-48 | -1.69E-46 |
| MS.gene051381 | MS.gene08887 | 0.822477 | 2.32E-53 | -1.69E-46 |
| MS.gene051642 | MS.gene08887 | 0.845388 | 4.19E-59 | -1.69E-46 |
| MS.gene051885 | MS.gene08887 | 0.821924 | 3.12E-53 | -1.69E-46 |
| MS.gene052242 | MS.gene08887 | -0.801148 | 1.03E-48 | -1.69E-46 |
| MS.gene052307 | MS.gene08887 | 0.826122 | 3.23E-54 | -1.69E-46 |
| MS.gene052397 | MS.gene08887 | 0.819341 | 1.22E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene08887 | 0.858358 | 8.70E-63 | -1.69E-46 |
| MS.gene053143 | MS.gene08887 | 0.88642 | 3.48E-72 | -1.69E-46 |
| MS.gene054585 | MS.gene08887 | 0.826787 | 2.24E-54 | -1.69E-46 |
| MS.gene054824 | MS.gene08887 | 0.806711 | 7.20E-50 | -1.69E-46 |
| MS.gene055469 | MS.gene08887 | 0.833483 | 5.20E-56 | -1.69E-46 |
| MS.gene056578 | MS.gene08887 | 0.804964 | 1.68E-49 | -1.69E-46 |
| MS.gene057213 | MS.gene08887 | 0.805625 | 1.22E-49 | -1.69E-46 |
| MS.gene058054 | MS.gene08887 | 0.823859 | 1.10E-53 | -1.69E-46 |
| MS.gene058638 | MS.gene08887 | 0.840583 | 7.97E-58 | -1.69E-46 |
| MS.gene058639 | MS.gene08887 | 0.825278 | 5.12E-54 | -1.69E-46 |
| MS.gene059007 | MS.gene08887 | 0.828728 | 7.65E-55 | -1.69E-46 |
| MS.gene059171 | MS.gene08887 | 0.849873 | 2.45E-60 | -1.69E-46 |
| MS.gene060563 | MS.gene08887 | 0.802259 | 6.11E-49 | -1.69E-46 |
| MS.gene061006 | MS.gene08887 | 0.876375 | 1.47E-68 | -1.69E-46 |
| MS.gene061100 | MS.gene08887 | 0.819246 | 1.28E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08887.t1 | MTR_8g079990 | 100.000 | 123 | 0 | 0 | 1 | 123 | 157 | 279 | 1.94e-83 | 257 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08887.t1 | AT1G67680 | 65.323 | 124 | 41 | 1 | 2 | 123 | 170 | 293 | 7.24e-49 | 165 |
| MS.gene08887.t1 | AT1G67650 | 56.557 | 122 | 49 | 2 | 2 | 123 | 163 | 280 | 7.52e-41 | 143 |
Find 28 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACATCTTTGGACCTTTC+AGG | 0.148742 | 4.4:-16681800 | None:intergenic |
| AGGATGGCTGGATTCATTTC+GGG | 0.250936 | 4.4:+16680837 | MS.gene08887:CDS |
| ATCATTGATTGGCAGCTTCT+TGG | 0.293542 | 4.4:+16681681 | MS.gene08887:intron |
| AAGGATGGCTGGATTCATTT+CGG | 0.297450 | 4.4:+16680836 | MS.gene08887:CDS |
| GTCTCCTATTGCTGTACAAT+TGG | 0.300746 | 4.4:+16681569 | MS.gene08887:CDS |
| CAGACTATCAGAAACATCTT+TGG | 0.317452 | 4.4:-16681810 | None:intergenic |
| TCATTGATTGGCAGCTTCTT+GGG | 0.354425 | 4.4:+16681682 | MS.gene08887:intron |
| AGCAACAAGCAGCTTTGAAT+TGG | 0.358344 | 4.4:+16680864 | MS.gene08887:CDS |
| TAGCCCAATTGTACAGCAAT+AGG | 0.402117 | 4.4:-16681573 | None:intergenic |
| GTTGCTGCCTTAGTTATGGC+AGG | 0.413143 | 4.4:+16680796 | None:intergenic |
| AAACATCTTTGGACCTTTCA+GGG | 0.418525 | 4.4:-16681799 | None:intergenic |
| GGACATGATTAAACGAGATA+TGG | 0.428299 | 4.4:+16681734 | MS.gene08887:CDS |
| TCTCCTATTGCTGTACAATT+GGG | 0.428740 | 4.4:+16681570 | MS.gene08887:CDS |
| AGAGCCTGATGGAAGATAAC+TGG | 0.433802 | 4.4:+16681520 | MS.gene08887:CDS |
| CTGCTTGTTCTTTAATTCAA+AGG | 0.451905 | 4.4:+16680896 | MS.gene08887:CDS |
| AATAATCTTGTTTCCCTGAA+AGG | 0.467300 | 4.4:+16681786 | MS.gene08887:CDS |
| ATCTGAAGTTCAAGGATGGC+TGG | 0.467862 | 4.4:+16680825 | MS.gene08887:CDS |
| ACAATTGGGCTATGTCCAAC+AGG | 0.482796 | 4.4:+16681584 | MS.gene08887:CDS |
| CAGGCCAGTTATCTTCCATC+AGG | 0.491778 | 4.4:-16681524 | None:intergenic |
| AGATGCTATTGAAGCTTATG+TGG | 0.514106 | 4.4:+16681713 | MS.gene08887:CDS |
| AACTGCAATGGACGATTCAT+CGG | 0.545482 | 4.4:-16681757 | None:intergenic |
| AGCTCTTGTTGTCCGCCCGA+AGG | 0.549212 | 4.4:+16680941 | MS.gene08887:CDS |
| TTGCTGCCTTAGTTATGGCA+GGG | 0.553077 | 4.4:+16680797 | None:intergenic |
| ATTATTGACTGCAACTGCAA+TGG | 0.572155 | 4.4:-16681769 | None:intergenic |
| GGGAGATCATCTGAAGTTCA+AGG | 0.584389 | 4.4:+16680817 | MS.gene08887:CDS |
| AATTGGTCAAGAGAGCCTGA+TGG | 0.624372 | 4.4:+16681509 | MS.gene08887:CDS |
| GATCATCTGAAGTTCAAGGA+TGG | 0.631439 | 4.4:+16680821 | MS.gene08887:CDS |
| AATATCACATCACCTTCGGG+CGG | 0.714098 | 4.4:-16680953 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAATTTATGACTTAAT+AGG | - | chr4.4:16681434-16681453 | None:intergenic | 10.0% |
| !!! | AAAATATCTGAAGTAAATTT+GGG | - | chr4.4:16681002-16681021 | None:intergenic | 15.0% |
| !!! | TATATTTTTTCTATCAGAAT+TGG | + | chr4.4:16681492-16681511 | MS.gene08887:intron | 15.0% |
| !! | AAGTCATAAATTTATTTCCT+GGG | + | chr4.4:16681437-16681456 | MS.gene08887:intron | 20.0% |
| !! | ATTGAATATTTGAATAGTGT+TGG | - | chr4.4:16681075-16681094 | None:intergenic | 20.0% |
| !! | TAAGTCATAAATTTATTTCC+TGG | + | chr4.4:16681436-16681455 | MS.gene08887:intron | 20.0% |
| !!! | AGTTTTAATCAATCATTGAT+TGG | + | chr4.4:16681670-16681689 | MS.gene08887:intron | 20.0% |
| !!! | ATTTACTTCAGATATTTTCA+TGG | + | chr4.4:16681004-16681023 | MS.gene08887:intron | 20.0% |
| !!! | GAAAATATCTGAAGTAAATT+TGG | - | chr4.4:16681003-16681022 | None:intergenic | 20.0% |
| !!! | TTTACTTCAGATATTTTCAT+GGG | + | chr4.4:16681005-16681024 | MS.gene08887:intron | 20.0% |
| ! | AAGTTCCTTATTGTAAAACA+TGG | + | chr4.4:16681342-16681361 | MS.gene08887:intron | 25.0% |
| !! | TACAATCAAATGTTGTTTGT+CGG | + | chr4.4:16681381-16681400 | MS.gene08887:intron | 25.0% |
| !! | TCATTTTCTATATCTTCATC+AGG | - | chr4.4:16681546-16681565 | None:intergenic | 25.0% |
| !! | TTTGTCCATGTTTTACAATA+AGG | - | chr4.4:16681350-16681369 | None:intergenic | 25.0% |
| CTGCTTGTTCTTTAATTCAA+AGG | + | chr4.4:16680896-16680915 | MS.gene08887:CDS | 30.0% | |
| TCAAAATATCACATCACCTT+CGG | - | chr4.4:16680960-16680979 | None:intergenic | 30.0% | |
| TCATAAATTTATTTCCTGGG+AGG | + | chr4.4:16681440-16681459 | MS.gene08887:intron | 30.0% | |
| ! | AATAATCTTGTTTCCCTGAA+AGG | + | chr4.4:16681786-16681805 | MS.gene08887:CDS | 30.0% |
| !!! | GTGATGTGATATTTTGATTG+TGG | + | chr4.4:16680963-16680982 | MS.gene08887:intron | 30.0% |
| AAACACATACTCGTGAAGTT+TGG | - | chr4.4:16681199-16681218 | None:intergenic | 35.0% | |
| AAACATCTTTGGACCTTTCA+GGG | - | chr4.4:16681802-16681821 | None:intergenic | 35.0% | |
| AGATGCTATTGAAGCTTATG+TGG | + | chr4.4:16681713-16681732 | MS.gene08887:CDS | 35.0% | |
| CAAAATATCACATCACCTTC+GGG | - | chr4.4:16680959-16680978 | None:intergenic | 35.0% | |
| CAGACTATCAGAAACATCTT+TGG | - | chr4.4:16681813-16681832 | None:intergenic | 35.0% | |
| GGACATGATTAAACGAGATA+TGG | + | chr4.4:16681734-16681753 | MS.gene08887:CDS | 35.0% | |
| TCTCCTATTGCTGTACAATT+GGG | + | chr4.4:16681570-16681589 | MS.gene08887:CDS | 35.0% | |
| ! | ATTATTGACTGCAACTGCAA+TGG | - | chr4.4:16681772-16681791 | None:intergenic | 35.0% |
| ! | CACGAAGCATCTATTACTAA+TGG | - | chr4.4:16681291-16681310 | None:intergenic | 35.0% |
| AAATCAGAACTCCTACCTGT+TGG | - | chr4.4:16681602-16681621 | None:intergenic | 40.0% | |
| AACTGCAATGGACGATTCAT+CGG | - | chr4.4:16681760-16681779 | None:intergenic | 40.0% | |
| AAGGATGGCTGGATTCATTT+CGG | + | chr4.4:16680836-16680855 | MS.gene08887:CDS | 40.0% | |
| GAAACATCTTTGGACCTTTC+AGG | - | chr4.4:16681803-16681822 | None:intergenic | 40.0% | |
| GTCTCCTATTGCTGTACAAT+TGG | + | chr4.4:16681569-16681588 | MS.gene08887:CDS | 40.0% | |
| GTTTGCAAGTCAAGTGTGAT+TGG | + | chr4.4:16681310-16681329 | MS.gene08887:intron | 40.0% | |
| TAGCCCAATTGTACAGCAAT+AGG | - | chr4.4:16681576-16681595 | None:intergenic | 40.0% | |
| ! | ATCATTGATTGGCAGCTTCT+TGG | + | chr4.4:16681681-16681700 | MS.gene08887:intron | 40.0% |
| ! | GATCATCTGAAGTTCAAGGA+TGG | + | chr4.4:16680821-16680840 | MS.gene08887:CDS | 40.0% |
| ! | TCATTGATTGGCAGCTTCTT+GGG | + | chr4.4:16681682-16681701 | MS.gene08887:intron | 40.0% |
| !! | AGCAACAAGCAGCTTTGAAT+TGG | + | chr4.4:16680864-16680883 | MS.gene08887:CDS | 40.0% |
| AATATCACATCACCTTCGGG+CGG | - | chr4.4:16680956-16680975 | None:intergenic | 45.0% | |
| AATTGGTCAAGAGAGCCTGA+TGG | + | chr4.4:16681509-16681528 | MS.gene08887:CDS | 45.0% | |
| ACAATTGGGCTATGTCCAAC+AGG | + | chr4.4:16681584-16681603 | MS.gene08887:CDS | 45.0% | |
| AGAGCCTGATGGAAGATAAC+TGG | + | chr4.4:16681520-16681539 | MS.gene08887:CDS | 45.0% | |
| AGGATGGCTGGATTCATTTC+GGG | + | chr4.4:16680837-16680856 | MS.gene08887:CDS | 45.0% | |
| CCATTCATGTATAACCTCCC+AGG | - | chr4.4:16681457-16681476 | None:intergenic | 45.0% | |
| CCTGGGAGGTTATACATGAA+TGG | + | chr4.4:16681454-16681473 | MS.gene08887:intron | 45.0% | |
| ! | ATCTGAAGTTCAAGGATGGC+TGG | + | chr4.4:16680825-16680844 | MS.gene08887:CDS | 45.0% |
| ! | GGGAGATCATCTGAAGTTCA+AGG | + | chr4.4:16680817-16680836 | MS.gene08887:CDS | 45.0% |
| CAGGCCAGTTATCTTCCATC+AGG | - | chr4.4:16681527-16681546 | None:intergenic | 50.0% | |
| TTGGGCTATGTCCAACAGGT+AGG | + | chr4.4:16681588-16681607 | MS.gene08887:intron | 50.0% | |
| ! | AGCTCTTGTTGTCCGCCCGA+AGG | + | chr4.4:16680941-16680960 | MS.gene08887:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 16680811 | 16681833 | 16680811 | ID=MS.gene08887 |
| chr4.4 | mRNA | 16680811 | 16681833 | 16680811 | ID=MS.gene08887.t1;Parent=MS.gene08887 |
| chr4.4 | exon | 16680811 | 16680962 | 16680811 | ID=MS.gene08887.t1.exon1;Parent=MS.gene08887.t1 |
| chr4.4 | CDS | 16680811 | 16680962 | 16680811 | ID=cds.MS.gene08887.t1;Parent=MS.gene08887.t1 |
| chr4.4 | exon | 16681509 | 16681605 | 16681509 | ID=MS.gene08887.t1.exon2;Parent=MS.gene08887.t1 |
| chr4.4 | CDS | 16681509 | 16681605 | 16681509 | ID=cds.MS.gene08887.t1;Parent=MS.gene08887.t1 |
| chr4.4 | exon | 16681696 | 16681833 | 16681696 | ID=MS.gene08887.t1.exon3;Parent=MS.gene08887.t1 |
| chr4.4 | CDS | 16681696 | 16681833 | 16681696 | ID=cds.MS.gene08887.t1;Parent=MS.gene08887.t1 |
| Gene Sequence |
| Protein sequence |
