Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21325.t1 | XP_003615289.2 | 92.9 | 225 | 12 | 1 | 4 | 224 | 1 | 225 | 1.50E-101 | 379 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21325.t1 | O82794 | 54.8 | 221 | 93 | 4 | 4 | 221 | 1 | 217 | 5.7e-49 | 195.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21325.t1 | G7KBL2 | 92.9 | 225 | 12 | 1 | 4 | 224 | 1 | 225 | 1.1e-101 | 379.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene21325 | 0.984643 | 3.67E-161 | -1.69E-46 |
| MS.gene050168 | MS.gene21325 | 0.8011 | 1.06E-48 | -1.69E-46 |
| MS.gene050426 | MS.gene21325 | -0.81377 | 2.16E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene21325 | 0.80813 | 3.60E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene21325 | 0.800569 | 1.36E-48 | -1.69E-46 |
| MS.gene051525 | MS.gene21325 | 0.814068 | 1.86E-51 | -1.69E-46 |
| MS.gene051544 | MS.gene21325 | 0.812211 | 4.75E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene21325 | 0.801711 | 7.92E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene21325 | 0.820772 | 5.75E-53 | -1.69E-46 |
| MS.gene052011 | MS.gene21325 | -0.874466 | 6.61E-68 | -1.69E-46 |
| MS.gene054183 | MS.gene21325 | 0.884935 | 1.25E-71 | -1.69E-46 |
| MS.gene054719 | MS.gene21325 | 0.80759 | 4.69E-50 | -1.69E-46 |
| MS.gene055217 | MS.gene21325 | 0.828623 | 8.11E-55 | -1.69E-46 |
| MS.gene055358 | MS.gene21325 | 0.800613 | 1.33E-48 | -1.69E-46 |
| MS.gene05553 | MS.gene21325 | 0.800796 | 1.22E-48 | -1.69E-46 |
| MS.gene056899 | MS.gene21325 | -0.836285 | 1.02E-56 | -1.69E-46 |
| MS.gene056979 | MS.gene21325 | 0.825203 | 5.33E-54 | -1.69E-46 |
| MS.gene057476 | MS.gene21325 | 0.811591 | 6.49E-51 | -1.69E-46 |
| MS.gene057888 | MS.gene21325 | 0.824534 | 7.66E-54 | -1.69E-46 |
| MS.gene058220 | MS.gene21325 | 0.809544 | 1.80E-50 | -1.69E-46 |
| MS.gene059247 | MS.gene21325 | 0.806816 | 6.84E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene21325 | 0.847037 | 1.49E-59 | -1.69E-46 |
| MS.gene060038 | MS.gene21325 | 0.822656 | 2.11E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene21325 | -0.845737 | 3.37E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGATTCTTAATGAAAT+TGG | 0.154424 | 5.1:+51301940 | MS.gene21325:CDS |
| TTGCAGAGGTTTATCCAATT+TGG | 0.271458 | 5.1:-51301049 | None:intergenic |
| TTACGTTAAGTTCTATCTTT+GGG | 0.294383 | 5.1:+51305181 | MS.gene21325:CDS |
| TCTGACACATCTCTTAAGTT+AGG | 0.297639 | 5.1:+51305297 | MS.gene21325:CDS |
| GTTACGTTAAGTTCTATCTT+TGG | 0.298906 | 5.1:+51305180 | MS.gene21325:CDS |
| AGAAGATTCTTAATGAAATT+GGG | 0.314719 | 5.1:+51301941 | MS.gene21325:CDS |
| GTCCCACCACATCACCAAAT+TGG | 0.337123 | 5.1:+51301035 | MS.gene21325:CDS |
| CCCAACATCCCAATTCAATT+AGG | 0.339905 | 5.1:-51305584 | None:intergenic |
| ACAGCTGCTTACATTATTCA+TGG | 0.347856 | 5.1:-51305244 | None:intergenic |
| AATATGTAGAGAAAGATTAA+TGG | 0.356186 | 5.1:+51291592 | None:intergenic |
| CATGCCAACTGAATTGTATA+AGG | 0.358067 | 5.1:+51301284 | MS.gene21325:CDS |
| CTTAGAGGGGTTGCAGCAAT+TGG | 0.364033 | 5.1:+51301473 | MS.gene21325:CDS |
| ATTGAGAAACCTTAGTTGTT+GGG | 0.403691 | 5.1:-51301322 | None:intergenic |
| TTGGAGAAAACTCTTGAATC+AGG | 0.405786 | 5.1:+51301492 | MS.gene21325:CDS |
| ATCCCAATTCAATTAGGAAA+TGG | 0.426908 | 5.1:-51305578 | None:intergenic |
| GAACAAGCATTTAAAGCAAA+AGG | 0.431342 | 5.1:+51305039 | MS.gene21325:CDS |
| AATTGAGAAACCTTAGTTGT+TGG | 0.432761 | 5.1:-51301323 | None:intergenic |
| AATTGGGGCACTTCGAATGA+AGG | 0.468411 | 5.1:+51301957 | MS.gene21325:CDS |
| ATGATACAACCCAACAACTA+AGG | 0.469146 | 5.1:+51301313 | MS.gene21325:CDS |
| TTCGGTTTGATATAGTATGA+AGG | 0.479546 | 5.1:+51300987 | MS.gene21325:intron |
| AAGCCGTACGTACTTGGAAC+TGG | 0.480819 | 5.1:-51291783 | None:intergenic |
| CTGACACATCTCTTAAGTTA+GGG | 0.489139 | 5.1:+51305298 | MS.gene21325:CDS |
| CAAGCGTGTGATTGAGATGA+AGG | 0.497959 | 5.1:+51301518 | MS.gene21325:CDS |
| TTTGAAGGGTTAAACTTAGA+GGG | 0.500607 | 5.1:+51301459 | MS.gene21325:CDS |
| TCGGTTTGATATAGTATGAA+GGG | 0.513655 | 5.1:+51300988 | MS.gene21325:intron |
| TATCCAATTTGGTGATGTGG+TGG | 0.522005 | 5.1:-51301038 | None:intergenic |
| TACTTTACCTGCACTTGCAG+AGG | 0.526172 | 5.1:-51301063 | None:intergenic |
| GGATAAACCTCTGCAAGTGC+AGG | 0.526573 | 5.1:+51301056 | MS.gene21325:CDS |
| ACTTCCTTATACAATTCAGT+TGG | 0.535258 | 5.1:-51301288 | None:intergenic |
| GAAGATTCTTAATGAAATTG+GGG | 0.550878 | 5.1:+51301942 | MS.gene21325:CDS |
| GAGGAATCATCTTCAAGAGA+AGG | 0.559575 | 5.1:-51305276 | None:intergenic |
| TTGAAGGGTTAAACTTAGAG+GGG | 0.563668 | 5.1:+51301460 | MS.gene21325:CDS |
| ATCCAATTTGGTGATGTGGT+GGG | 0.563973 | 5.1:-51301037 | None:intergenic |
| GTTTATCCAATTTGGTGATG+TGG | 0.574639 | 5.1:-51301041 | None:intergenic |
| AATGTAAGCAGCTGTAATAG+TGG | 0.579600 | 5.1:+51305252 | MS.gene21325:CDS |
| TACGTTAAGTTCTATCTTTG+GGG | 0.585861 | 5.1:+51305182 | MS.gene21325:CDS |
| AAAGGAAATCATGCTAGAAG+AGG | 0.586650 | 5.1:+51305015 | MS.gene21325:intron |
| AGGAATCATCTTCAAGAGAA+GGG | 0.588663 | 5.1:-51305275 | None:intergenic |
| GTATGAAGGGCATAATTACA+AGG | 0.624293 | 5.1:+51301001 | MS.gene21325:intron |
| GTAGAGAAAGATTAATGGCG+AGG | 0.644027 | 5.1:+51291597 | None:intergenic |
| AAATAGATAATGCAACTGCA+AGG | 0.650419 | 5.1:+51291639 | MS.gene21325:CDS |
| TAACTTAAGAGATGTGTCAG+AGG | 0.666354 | 5.1:-51305295 | None:intergenic |
| AGGGATCTTCAAGAAAGCTG+AGG | 0.666401 | 5.1:+51291688 | MS.gene21325:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 51291601 | 51305592 | 51291601 | ID=MS.gene21325 |
| chr5.1 | mRNA | 51291601 | 51305592 | 51291601 | ID=MS.gene21325.t1;Parent=MS.gene21325 |
| chr5.1 | exon | 51291601 | 51291792 | 51291601 | ID=MS.gene21325.t1.exon1;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51291601 | 51291792 | 51291601 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| chr5.1 | exon | 51301002 | 51301077 | 51301002 | ID=MS.gene21325.t1.exon2;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51301002 | 51301077 | 51301002 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| chr5.1 | exon | 51301273 | 51301334 | 51301273 | ID=MS.gene21325.t1.exon3;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51301273 | 51301334 | 51301273 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| chr5.1 | exon | 51301440 | 51301539 | 51301440 | ID=MS.gene21325.t1.exon4;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51301440 | 51301539 | 51301440 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| chr5.1 | exon | 51301937 | 51301978 | 51301937 | ID=MS.gene21325.t1.exon5;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51301937 | 51301978 | 51301937 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| chr5.1 | exon | 51305019 | 51305060 | 51305019 | ID=MS.gene21325.t1.exon6;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51305019 | 51305060 | 51305019 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| chr5.1 | exon | 51305177 | 51305319 | 51305177 | ID=MS.gene21325.t1.exon7;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51305177 | 51305319 | 51305177 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| chr5.1 | exon | 51305574 | 51305592 | 51305574 | ID=MS.gene21325.t1.exon8;Parent=MS.gene21325.t1 |
| chr5.1 | CDS | 51305574 | 51305592 | 51305574 | ID=cds.MS.gene21325.t1;Parent=MS.gene21325.t1 |
| Gene Sequence |
| Protein sequence |
