AlfalfaGEDB | MS.gene21852

M. sativa cultivar XinJiangDaYe / MS.gene21852

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21852.t1	RHN53387.1	95.2	480	22	1	1	480	29	507	8.60E-264	919.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21852.t1	Q8VWZ7	51.9	476	223	4	3	478	24	493	2.1e-141	503.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21852.t1	G7K567	95.2	480	22	1	1	480	1	479	6.2e-264	919.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene001721	MS.gene21852	PPI
MS.gene002268	MS.gene21852	PPI
MS.gene002271	MS.gene21852	PPI
MS.gene002272	MS.gene21852	PPI
MS.gene002273	MS.gene21852	PPI
MS.gene005830	MS.gene21852	PPI
MS.gene006453	MS.gene21852	PPI
MS.gene006454	MS.gene21852	PPI
MS.gene00811	MS.gene21852	PPI
MS.gene010418	MS.gene21852	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene21852.t1	MTR_5g007460	95.208	480	22	1	1	480	1	479	936
MS.gene21852.t1	MTR_5g007450	88.542	480	42	3	1	480	34	500	862
MS.gene21852.t1	MTR_5g007550	87.708	480	46	3	1	480	34	500	857
MS.gene21852.t1	MTR_1g103670	59.283	474	183	4	4	475	29	494	595
MS.gene21852.t1	MTR_1g031700	58.542	480	189	4	3	480	28	499	590
MS.gene21852.t1	MTR_8g046240	56.341	481	202	3	3	479	31	507	586
MS.gene21852.t1	MTR_1g103680	59.833	478	182	4	4	479	30	499	580
MS.gene21852.t1	MTR_2g073420	58.017	474	180	5	6	479	41	495	577
MS.gene21852.t1	MTR_8g046210	56.341	481	202	3	3	479	31	507	564
MS.gene21852.t1	MTR_2g073440	57.883	463	170	5	4	466	28	465	561

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21852.t1	AT2G45550	45.684	475	250	5	7	477	36	506	8.66e-156	452
MS.gene21852.t1	AT1G33720	45.684	475	250	5	7	477	36	506	1.38e-155	452
MS.gene21852.t1	AT2G45560	45.094	479	255	5	3	477	32	506	3.06e-155	451
MS.gene21852.t1	AT2G45570	48.172	465	232	6	18	477	47	507	3.34e-155	451
MS.gene21852.t1	AT3G61040	46.482	469	241	6	11	477	35	495	3.63e-154	448
MS.gene21852.t1	AT2G45580	46.767	464	238	5	19	477	48	507	4.37e-152	443
MS.gene21852.t1	AT1G33720	46.154	377	196	4	104	477	2	374	1.57e-123	365
MS.gene21852.t1	AT1G33720	46.154	377	196	4	104	477	2	374	1.57e-123	365
MS.gene21852.t1	AT1G33720	46.154	377	196	4	104	477	2	374	1.57e-123	365
MS.gene21852.t1	AT1G33730	46.175	366	186	5	111	471	5	364	7.20e-121	358

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Find 115 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGATGTAGAACTCAA+TGG	0.537607	5.1:+1890257	MS.gene21852:CDS
AAATAAAGAGAATAAGGATT+TGG	0.376395	5.1:+1889299	MS.gene21852:CDS
AAATCCAAATGTAATGTCAA+AGG	0.550896	5.1:+1890091	MS.gene21852:CDS
AACAACCATTGTTGTCTCTT+CGG	0.458243	5.1:+1888918	MS.gene21852:CDS
AACCACGACCAGTTTGGGGT+TGG	0.517579	5.1:+1889021	MS.gene21852:CDS
AACTACATATACATTAGAAT+GGG	0.484037	5.1:+1890049	MS.gene21852:CDS
AAGGAACTTGAAGAAATCAT+TGG	0.438074	5.1:+1890119	MS.gene21852:CDS
AAGGGTGAAGCAATAGATAT+GGG	0.513214	5.1:+1889189	MS.gene21852:CDS
AATTTGTCCCGGCTTGCCAT+TGG	0.482748	5.1:+1890448	MS.gene21852:CDS
ACAAACTCAAAACTTCTACC+AGG	0.631797	5.1:+1888778	MS.gene21852:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTGATGTAGAACTCAA+TGG	+	chr5.1:1890257-1890276	MS.gene21852:CDS	30.0%
AAGGAACTTGAAGAAATCAT+TGG	+	chr5.1:1890119-1890138	MS.gene21852:CDS	30.0%
ACAAATCTTCGTTAATGTGT+GGG	+	chr5.1:1890301-1890320	MS.gene21852:CDS	30.0%
AGTTTGGCTAATGATTTATG+TGG	-	chr5.1:1888853-1888872	None:intergenic	30.0%
ATATACATTAGAATGGGCAA+TGG	+	chr5.1:1890055-1890074	MS.gene21852:CDS	30.0%
ATGAGTGTAATAGTAGTAAG+GGG	-	chr5.1:1889609-1889628	None:intergenic	30.0%
CTCTCTGGTGAAAATAAATA+TGG	-	chr5.1:1890359-1890378	None:intergenic	30.0%
CTTGAAGAAATCATTGGAAT+AGG	+	chr5.1:1890125-1890144	MS.gene21852:CDS	30.0%
GATCAATCAACCTCAATAAT+GGG	-	chr5.1:1889374-1889393	None:intergenic	30.0%
GCTTATTGTTCATTACAAAG+AGG	-	chr5.1:1888831-1888850	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	1888766	1890637	1888766	ID=MS.gene21852
chr5.1	mRNA	1888766	1890637	1888766	ID=MS.gene21852.t1;Parent=MS.gene21852
chr5.1	exon	1888766	1889593	1888766	ID=MS.gene21852.t1.exon1;Parent=MS.gene21852.t1
chr5.1	CDS	1888766	1889593	1888766	ID=cds.MS.gene21852.t1;Parent=MS.gene21852.t1
chr5.1	exon	1890023	1890637	1890023	ID=MS.gene21852.t1.exon2;Parent=MS.gene21852.t1
chr5.1	CDS	1890023	1890637	1890023	ID=cds.MS.gene21852.t1;Parent=MS.gene21852.t1

Gene Sequence

>MS.gene21852

ATGAGAAACAAAACAAACTCAAAACTTCTACCAGGACCTTTCCCTCTTCCCATAGTTGGAAACCTCTTTGTAATGAACAATAAGCCACATAAATCATTAGCCAAACTTGCCAAAATTTATGGTCCAATATTAAGTTTAAAACTAGGCCAAGTAACAACCATTGTTGTCTCTTCGGCCGACATGGCCAAAGAGATACTCCAAACTCATGATTCCTTACTCTCAGACCGGACCGTACCTCATGCTTTAACGGCTTTCAACCACGACCAGTTTGGGGTTGGATTCCTCCCTCTTTCGCCTCTTTGGCGAGAGATGAGAAAAGTATGCAAAAATCAACTTTTCTCCAACAAGAGTCTTGATGCAAACCAATGTATTAGGCGTAAAAAGATAGATGAACTCATCAGCTATGTTTCTCAAAGGAGCCTTAAGGGTGAAGCAATAGATATGGGAAAAGTAGCTTTTAGGACTTCAATTAATTTGTTGTCAAATACTATTTTTTCTGTAGACTTTGCTAATAACTCTGCAGGTACTAATGAAAATAAAGAGAATAAGGATTTGGTTATGAATATGGCAGAGACGGTTGGAAAGCCAAACATGGCTGATTTTTTCCCATTATTGAGGTTGATTGATCCACAAGGCATCAAAAAGACCTATATGTTTTATATTGGAAAGTTGTTCAATGTCTTCGACAACATAATCGACCAAAGGTTGAAGTTGCGAGAAGGAGATGGTTTTGTCACCAACAACGACATGCTAGACTCCTTGCTCGACATTCCAGAAGAAAACGGAAAGGAGTTGTTGGATAGAGAAAAAATTGAACATTTATTACATGTATTTTCTAATCCCCTTACTACTATTACACTCATATTGTTTGATATCTCTTTCTCTTGTCCACTTTATCTACGTCTCTCTCTGTGGACTTGGATTCTTTCCGCTCCCTTCATTCTCTAAGGCTAATCCGTCGGATTAATCTAACTACAGAGAGTGTTGATATCTCTGCTACTAGATTAATCAGACAGATGAGTTTTTATTGAAGAAACAGGAGGGACAAATAAACATTCTAAATATCTGTGTGTGCGCACTTGTGCGCATCGATTGATATCTTATGTCATGCTTCAATTTTTAATAATGTTTTAATAAAGTTATATGATGTAGAATCAATTATTTTTTGAACTATATGAACTTTTGTACATTTTATTTATTTATAAAAAATTTCAATCTAATAGATTATATAACTATTGTTACCATTGTTTATAACAGGATCTACTTGTTGGAGGAACTGATACAACTACATATACATTAGAATGGGCAATGGCTGAACTACTTCAAAATCCAAATGTAATGTCAAAGGCCAAAAAGGAACTTGAAGAAATCATTGGAATAGGAAATCCAATTGAGGAATCTGATATTACTAGACTCCCTTACTTGCAAGCAATAATAAAGGAAACCTTACGCTTACACCCGATAGCTCCACTTTTGCTCCCCAGAAAAGCAAAAACTGATGTAGAACTCAATGGCTACACCATTCCGAAAGATGCACAAATCTTCGTTAATGTGTGGGATATTGGTAGAGACCCGGAAGTATGGGATAATCCATATTTATTTTCACCAGAGAGGTTTTTGGGAACAAAGCTCGATATCAAGGGCCAAAATTTTCAGCTCACACCGTTTGGCAGTGGAAGGCGAATTTGTCCCGGCTTGCCATTGGCTATGAGGATGTTGCACATGATGTTGGGATCATTGCTAAATTCTTTTGATTGGAAACTAGAAAATGATATGAAACCTGAAGAAATAGACATGGAAGATGCAATACAAGGACTTGCCTTGAGAAAATGTGAACCCTTGAGAGTTGTTCCAACCAAAATCAGTAATTAA

Protein sequence

>MS.gene21852.t1

MRNKTNSKLLPGPFPLPIVGNLFVMNNKPHKSLAKLAKIYGPILSLKLGQVTTIVVSSADMAKEILQTHDSLLSDRTVPHALTAFNHDQFGVGFLPLSPLWREMRKVCKNQLFSNKSLDANQCIRRKKIDELISYVSQRSLKGEAIDMGKVAFRTSINLLSNTIFSVDFANNSAGTNENKENKDLVMNMAETVGKPNMADFFPLLRLIDPQGIKKTYMFYIGKLFNVFDNIIDQRLKLREGDGFVTNNDMLDSLLDIPEENGKELLDREKIEHLLHDLLVGGTDTTTYTLEWAMAELLQNPNVMSKAKKELEEIIGIGNPIEESDITRLPYLQAIIKETLRLHPIAPLLLPRKAKTDVELNGYTIPKDAQIFVNVWDIGRDPEVWDNPYLFSPERFLGTKLDIKGQNFQLTPFGSGRRICPGLPLAMRMLHMMLGSLLNSFDWKLENDMKPEEIDMEDAIQGLALRKCEPLRVVPTKISN