Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23953.t1 | XP_003597891.1 | 99.1 | 106 | 1 | 0 | 1 | 106 | 19 | 124 | 1.10E-54 | 222.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23953.t1 | C0HLF1 | 32.4 | 105 | 70 | 1 | 1 | 104 | 20 | 124 | 1.9e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23953.t1 | Q2HUA7 | 99.1 | 106 | 1 | 0 | 1 | 106 | 19 | 124 | 8.0e-55 | 222.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene23953 | 0.802131 | 6.49E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene23953 | 0.811379 | 7.21E-51 | -1.69E-46 |
| MS.gene050780 | MS.gene23953 | 0.804894 | 1.73E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene23953 | 0.804539 | 2.06E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene23953 | 0.801882 | 7.30E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene23953 | 0.811358 | 7.29E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene23953 | 0.803382 | 3.58E-49 | -1.69E-46 |
| MS.gene051757 | MS.gene23953 | 0.810467 | 1.14E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene23953 | 0.804087 | 2.56E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene23953 | 0.812326 | 4.49E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene23953 | 0.811624 | 6.38E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene23953 | 0.815071 | 1.12E-51 | -1.69E-46 |
| MS.gene05364 | MS.gene23953 | 0.804485 | 2.11E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene23953 | 0.800324 | 1.52E-48 | -1.69E-46 |
| MS.gene055512 | MS.gene23953 | 0.809706 | 1.66E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene23953 | 0.819436 | 1.16E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene23953 | 0.806818 | 6.84E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene23953 | 0.803088 | 4.12E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene23953 | 0.819414 | 1.18E-52 | -1.69E-46 |
| MS.gene057140 | MS.gene23953 | 0.811496 | 6.80E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene23953 | 0.829949 | 3.87E-55 | -1.69E-46 |
| MS.gene059317 | MS.gene23953 | 0.81074 | 9.92E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene23953 | 0.801096 | 1.06E-48 | -1.69E-46 |
| MS.gene060429 | MS.gene23953 | 0.820456 | 6.79E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene23953 | 0.804858 | 1.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 127 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATCATTAATGAAAAATT+TGG | + | 15455:10485-10504 | MS.gene23953:intron | 10.0% |
| !! | ACATATTTAATCAAAAAACA+AGG | + | 15455:11808-11827 | MS.gene23953:intron | 15.0% |
| !! | TATCCTATTAGAAATAAATA+GGG | + | 15455:11051-11070 | MS.gene23953:intron | 15.0% |
| !! | TTAAATATGTATACTATTCT+TGG | - | 15455:11798-11817 | MS.gene23953:intergenic | 15.0% |
| !! | AATAACTAAAATTTAGTCCT+TGG | - | 15455:10291-10310 | MS.gene23953:intergenic | 20.0% |
| !! | AGTATTCTATCTTTGTAATT+GGG | - | 15455:11083-11102 | MS.gene23953:intergenic | 20.0% |
| !! | ATAAATCACTGTTTACTAAA+AGG | - | 15455:10699-10718 | MS.gene23953:intergenic | 20.0% |
| !! | GTATCCTATTAGAAATAAAT+AGG | + | 15455:11050-11069 | MS.gene23953:intron | 20.0% |
| !! | TAAATCACTGTTTACTAAAA+GGG | - | 15455:10698-10717 | MS.gene23953:intergenic | 20.0% |
| !! | TAGTATTCTATCTTTGTAAT+TGG | - | 15455:11084-11103 | MS.gene23953:intergenic | 20.0% |
| !! | TGTCATCAAATAAAAAAGAT+TGG | - | 15455:10848-10867 | MS.gene23953:intergenic | 20.0% |
| !! | TTATTAGAATTCTATTTGCA+AGG | + | 15455:10306-10325 | MS.gene23953:intron | 20.0% |
| !! | TTCATATAAACAGATTTCAA+CGG | - | 15455:10340-10359 | MS.gene23953:intergenic | 20.0% |
| !!! | ATGTGTTGATTATATTTTGT+TGG | + | 15455:11359-11378 | MS.gene23953:intron | 20.0% |
| !!! | ATTTATTTCTAATAGGATAC+AGG | - | 15455:11050-11069 | MS.gene23953:intergenic | 20.0% |
| !!! | GACATAATTTTCAAAAATGA+AGG | - | 15455:10650-10669 | MS.gene23953:intergenic | 20.0% |
| !!! | TTGTTATTTAAGAGAGAATT+GGG | - | 15455:11145-11164 | MS.gene23953:intergenic | 20.0% |
| !!! | TTTATTTCTAATAGGATACA+GGG | - | 15455:11049-11068 | MS.gene23953:intergenic | 20.0% |
| !!! | TTTTATTTGATGACAGTAAA+TGG | + | 15455:10853-10872 | MS.gene23953:intron | 20.0% |
| ! | AAAAATTTGGAAAAGAGTTG+TGG | + | 15455:10498-10517 | MS.gene23953:intron | 25.0% |
| ! | AAATTCTTCCATACTTATCA+AGG | - | 15455:12101-12120 | MS.gene23953:intergenic | 25.0% |
| ! | ATGGATAAATTACCTTATCT+AGG | + | 15455:10231-10250 | MS.gene23953:intron | 25.0% |
| ! | CAAAATATAATCAACACATC+AGG | - | 15455:11358-11377 | MS.gene23953:intergenic | 25.0% |
| ! | GATGATAAACTAGCTATTAA+AGG | + | 15455:11641-11660 | MS.gene23953:intron | 25.0% |
| ! | GTATTCTATCTTTGTAATTG+GGG | - | 15455:11082-11101 | MS.gene23953:intergenic | 25.0% |
| ! | TATATTGTATACACAGTACT+TGG | + | 15455:10756-10775 | MS.gene23953:CDS | 25.0% |
| ! | TTCTACTTCTTATCTTTATC+AGG | + | 15455:10727-10746 | MS.gene23953:intron | 25.0% |
| !! | CTAGTTTATCATCTTTTCTA+GGG | - | 15455:11634-11653 | MS.gene23953:intergenic | 25.0% |
| !! | TGTTATTTAAGAGAGAATTG+GGG | - | 15455:11144-11163 | MS.gene23953:intergenic | 25.0% |
| !!! | ATCTGTTACTGATTTTTGAT+GGG | - | 15455:10557-10576 | MS.gene23953:intergenic | 25.0% |
| !!! | CTTGTTATTTAAGAGAGAAT+TGG | - | 15455:11146-11165 | MS.gene23953:intergenic | 25.0% |
| !!! | TATCTGTTACTGATTTTTGA+TGG | - | 15455:10558-10577 | MS.gene23953:intergenic | 25.0% |
| !!! | TGTCCCTATTTATTTCTAAT+AGG | - | 15455:11057-11076 | MS.gene23953:intergenic | 25.0% |
| AAATTGCAATTTCAAACCCT+TGG | - | 15455:11917-11936 | MS.gene23952:intergenic | 30.0% | |
| AACTTTATCTGATTCTGAAC+TGG | + | 15455:10264-10283 | MS.gene23953:intron | 30.0% | |
| AATATAATCAACACATCAGG+AGG | - | 15455:11355-11374 | MS.gene23953:intergenic | 30.0% | |
| AATTCATTGCTAAACGAGAA+TGG | - | 15455:11608-11627 | MS.gene23953:intergenic | 30.0% | |
| AATTCTATTTGCAAGGTTAG+TGG | + | 15455:10313-10332 | MS.gene23953:intron | 30.0% | |
| AGAGAAGCTTGCAAAAATTA+AGG | - | 15455:11564-11583 | MS.gene23953:intergenic | 30.0% | |
| ATTAAGGCAATAGATCGAAA+AGG | - | 15455:11548-11567 | MS.gene23953:intergenic | 30.0% | |
| ATTCTATTTGCAAGGTTAGT+GGG | + | 15455:10314-10333 | MS.gene23953:intron | 30.0% | |
| GATAGAATACTACAGAACTT+CGG | + | 15455:11092-11111 | MS.gene23953:intron | 30.0% | |
| GTATGAATGTGTATCTTACA+TGG | + | 15455:10212-10231 | MS.gene23953:intron | 30.0% | |
| TAACAGAGAAGAATCAAACT+TGG | - | 15455:11393-11412 | MS.gene23953:intergenic | 30.0% | |
| TCGTTCATATTGTGATTTGA+TGG | + | 15455:11835-11854 | MS.gene23953:intron | 30.0% | |
| TGTGTTTGGATGAAATTGTA+TGG | + | 15455:12124-12143 | MS.gene23953:CDS | 30.0% | |
| TTCCATACTTATCAAGGAAA+CGG | - | 15455:12095-12114 | MS.gene23953:intergenic | 30.0% | |
| TTGCCTATAAATAACAAGAG+TGG | - | 15455:11186-11205 | MS.gene23953:intergenic | 30.0% | |
| ! | AACCTACATTGTTGATGAAA+GGG | - | 15455:11264-11283 | MS.gene23953:intergenic | 30.0% |
| ! | AAGGGTTTGAAATTGCAATT+TGG | + | 15455:11916-11935 | MS.gene23953:intron | 30.0% |
| !! | AGCATATTGTTATTTCACCA+AGG | + | 15455:11897-11916 | MS.gene23953:intron | 30.0% |
| !! | GCATATTGTTATTTCACCAA+GGG | + | 15455:11898-11917 | MS.gene23953:intron | 30.0% |
| !! | GCTAGTTTATCATCTTTTCT+AGG | - | 15455:11635-11654 | MS.gene23953:intergenic | 30.0% |
| AACAATATGCTTGAGTCACT+CGG | - | 15455:11888-11907 | MS.gene23952:intergenic | 35.0% | |
| AATAATCAGAACTGCAGACA+AGG | - | 15455:11304-11323 | MS.gene23953:intergenic | 35.0% | |
| ACTAGGAGCTGATAATCTTA+AGG | + | 15455:10108-10127 | MS.gene23953:CDS | 35.0% | |
| AGATGTCAGTATATACGTTG+TGG | + | 15455:10779-10798 | MS.gene23953:CDS | 35.0% | |
| AGTTAGAAAAGCTAGCTATC+AGG | + | 15455:11710-11729 | MS.gene23953:intron | 35.0% | |
| ATGTCAGTATATACGTTGTG+GGG | + | 15455:10781-10800 | MS.gene23953:CDS | 35.0% | |
| CAGTAACAGATACAAGATTG+TGG | + | 15455:10566-10585 | MS.gene23953:intron | 35.0% | |
| CTAGGAGCTGATAATCTTAA+GGG | + | 15455:10109-10128 | MS.gene23953:CDS | 35.0% | |
| GATGTCAGTATATACGTTGT+GGG | + | 15455:10780-10799 | MS.gene23953:CDS | 35.0% | |
| GCTATTAAAGGATACTCTTG+CGG | + | 15455:11653-11672 | MS.gene23953:intron | 35.0% | |
| TACAATAACCTCAGCTGTAA+AGG | + | 15455:12041-12060 | MS.gene23953:CDS | 35.0% | |
| TTGACAGCTTACGAACATAT+AGG | - | 15455:11947-11966 | MS.gene23953:intergenic | 35.0% | |
| TTGGAAAAGAGTTGTGGAAA+TGG | + | 15455:10504-10523 | MS.gene23953:intron | 35.0% | |
| ! | GAACCTACATTGTTGATGAA+AGG | - | 15455:11265-11284 | MS.gene23953:intergenic | 35.0% |
| ! | TGGAAGAATTTGCTTGTGTT+TGG | + | 15455:12110-12129 | MS.gene23953:CDS | 35.0% |
| ! | TTAAAGTGCTCACCTAGATA+AGG | - | 15455:10246-10265 | MS.gene23953:intergenic | 35.0% |
| ! | TTGTCTGCAGTTCTGATTAT+TGG | + | 15455:11303-11322 | MS.gene23953:intron | 35.0% |
| !! | CGACCACTCTTGTTATTTAT+AGG | + | 15455:11180-11199 | MS.gene23953:intron | 35.0% |
| !!! | GTTTTGTTTTTCTCCAGTGT+CGG | + | 15455:12008-12027 | MS.gene23953:intron | 35.0% |
| AAATAACAAGAGTGGTCGAG+AGG | - | 15455:11178-11197 | MS.gene23952:intergenic | 40.0% | |
| AAGAGAGAATTGGGGACTTT+GGG | - | 15455:11136-11155 | MS.gene23953:intergenic | 40.0% | |
| AATAACAAGAGTGGTCGAGA+GGG | - | 15455:11177-11196 | MS.gene23953:intergenic | 40.0% | |
| ACATACATCCTTTACAGCTG+AGG | - | 15455:12052-12071 | MS.gene23953:intergenic | 40.0% | |
| AGCCCTTTCATCAACAATGT+AGG | + | 15455:11259-11278 | MS.gene23953:intron | 40.0% | |
| AGCTATCAGGTGAACTAGTA+GGG | + | 15455:11723-11742 | MS.gene23953:intron | 40.0% | |
| ATGGGAAATATTGTGCAGGA+TGG | - | 15455:11483-11502 | MS.gene23953:intergenic | 40.0% | |
| CAGCTGTAAAGGATGTATGT+GGG | + | 15455:12052-12071 | MS.gene23953:CDS | 40.0% | |
| CGTTCTTTCTTGGTCAAACT+AGG | + | 15455:10091-10110 | MS.gene23953:CDS | 40.0% | |
| GAGAGCAGTATACTTACTTG+TGG | - | 15455:10165-10184 | MS.gene23953:intergenic | 40.0% | |
| GGTAACAAGGTTAGGTAGTT+GGG | - | 15455:11236-11255 | MS.gene23953:intergenic | 40.0% | |
| TAAGAGAGAATTGGGGACTT+TGG | - | 15455:11137-11156 | MS.gene23953:intergenic | 40.0% | |
| TAGCTATCAGGTGAACTAGT+AGG | + | 15455:11722-11741 | MS.gene23953:intron | 40.0% | |
| TCAGCTGTAAAGGATGTATG+TGG | + | 15455:12051-12070 | MS.gene23953:CDS | 40.0% | |
| TCATTGCTAAACGAGAATGG+TGG | - | 15455:11605-11624 | MS.gene23953:intergenic | 40.0% | |
| TGGTAACAAGGTTAGGTAGT+TGG | - | 15455:11237-11256 | MS.gene23953:intergenic | 40.0% | |
| TGGTATGGGAAATATTGTGC+AGG | - | 15455:11487-11506 | MS.gene23953:intergenic | 40.0% | |
| TTCTCCTCATGCTGTCAATT+GGG | + | 15455:11441-11460 | MS.gene23953:intron | 40.0% | |
| ! | ATTGTTGATGAAAGGGCTGT+TGG | - | 15455:11257-11276 | MS.gene23953:intergenic | 40.0% |
| ! | CTCTTGCGGTGTTGAAAATA+TGG | + | 15455:11667-11686 | MS.gene23953:intron | 40.0% |
| !! | AAACGAGAATGGTGGATTGA+GGG | - | 15455:11597-11616 | MS.gene23953:intergenic | 40.0% |
| !! | TAAACGAGAATGGTGGATTG+AGG | - | 15455:11598-11617 | MS.gene23953:intergenic | 40.0% |
| !!! | GGACGCTAGTTTTATGGTAT+GGG | - | 15455:11501-11520 | MS.gene23953:intergenic | 40.0% |
| !!! | TGGACGCTAGTTTTATGGTA+TGG | - | 15455:11502-11521 | MS.gene23953:intergenic | 40.0% |
| ACGTACACATGACTTGAGAG+AGG | - | 15455:10189-10208 | MS.gene23953:intergenic | 45.0% | |
| CAGTATATACGTTGTGGGGA+AGG | + | 15455:10785-10804 | MS.gene23953:CDS | 45.0% | |
| CGCCGTTTCCTTGATAAGTA+TGG | + | 15455:12090-12109 | MS.gene23953:CDS | 45.0% | |
| CTTACTTGTGGCAAGCAACT+AGG | - | 15455:10153-10172 | MS.gene23953:intergenic | 45.0% | |
| CTTCTCCTCATGCTGTCAAT+TGG | + | 15455:11440-11459 | MS.gene23953:intron | 45.0% | |
| GAAACAGAGCAACAGTGTTG+TGG | - | 15455:11526-11545 | MS.gene23953:intergenic | 45.0% | |
| GCAGGATGGACAAGATTCTA+GGG | - | 15455:11469-11488 | MS.gene23953:intergenic | 45.0% | |
| GCTATCAGGTGAACTAGTAG+GGG | + | 15455:11724-11743 | MS.gene23953:intron | 45.0% | |
| GTAAAGATAACCTCCGACAC+TGG | - | 15455:12024-12043 | MS.gene23953:intergenic | 45.0% | |
| TCTGATTCTGAACTGGTCCA+AGG | + | 15455:10271-10290 | MS.gene23953:intron | 45.0% | |
| TGCAGGATGGACAAGATTCT+AGG | - | 15455:11470-11489 | MS.gene23953:intergenic | 45.0% | |
| ! | TTTTCATGGAGTTCCTGCAG+AGG | + | 15455:10054-10073 | MS.gene23953:CDS | 45.0% |
| !! | AACGAGAATGGTGGATTGAG+GGG | - | 15455:11596-11615 | MS.gene23953:intergenic | 45.0% |
| !! | TGAAAGGGCTGTTGGTAACA+AGG | - | 15455:11249-11268 | MS.gene23953:intergenic | 45.0% |
| !! | TTGTTTTTCTCCAGTGTCGG+AGG | + | 15455:12011-12030 | MS.gene23953:intron | 45.0% |
| !!! | GTGGATGGACGCTAGTTTTA+TGG | - | 15455:11507-11526 | MS.gene23953:intergenic | 45.0% |
| ATGAGGAGAAGCAAAGCCAC+AGG | - | 15455:11431-11450 | MS.gene23953:intergenic | 50.0% | |
| ATTGTTGTGCCTAGCACCTG+TGG | + | 15455:11412-11431 | MS.gene23953:intron | 50.0% | |
| CAGAGCAACAGTGTTGTGGA+TGG | - | 15455:11522-11541 | MS.gene23953:intergenic | 50.0% | |
| CCAAGAAAGAACGCCAGTGT+AGG | - | 15455:10084-10103 | MS.gene23953:intergenic | 50.0% | |
| CCTACACTGGCGTTCTTTCT+TGG | + | 15455:10081-10100 | MS.gene23953:CDS | 50.0% | |
| GGAACCCAATTGACAGCATG+AGG | - | 15455:11448-11467 | MS.gene23953:intergenic | 50.0% | |
| TGCTTGAGTCACTCGGCAAT+TGG | - | 15455:11881-11900 | MS.gene23953:intergenic | 50.0% | |
| !! | GGGCTGTTGGTAACAAGGTT+AGG | - | 15455:11244-11263 | MS.gene23953:intergenic | 50.0% |
| AAGGAAACGGCGCTCAGTTG+GGG | - | 15455:12082-12101 | MS.gene23953:intergenic | 55.0% | |
| CAAGGAAACGGCGCTCAGTT+GGG | - | 15455:12083-12102 | MS.gene23953:intergenic | 55.0% | |
| GAAGCAAAGCCACAGGTGCT+AGG | - | 15455:11424-11443 | MS.gene23953:intergenic | 55.0% | |
| TCAAGGAAACGGCGCTCAGT+TGG | - | 15455:12084-12103 | MS.gene23953:intergenic | 55.0% | |
| AGGAAACGGCGCTCAGTTGG+GGG | - | 15455:12081-12100 | MS.gene23953:intergenic | 60.0% | |
| CAGTGTAGGCGCTCCTCTGC+AGG | - | 15455:10070-10089 | MS.gene23953:intergenic | 65.0% | |
| !! | CTGCAGAGGAGCGCCTACAC+TGG | + | 15455:10068-10087 | MS.gene23953:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15455 | gene | 10054 | 12149 | 10054 | ID=MS.gene23953 |
| 15455 | mRNA | 10054 | 12149 | 10054 | ID=MS.gene23953.t1;Parent=MS.gene23953 |
| 15455 | exon | 10054 | 10167 | 10054 | ID=MS.gene23953.t1.exon1;Parent=MS.gene23953.t1 |
| 15455 | CDS | 10054 | 10167 | 10054 | ID=cds.MS.gene23953.t1;Parent=MS.gene23953.t1 |
| 15455 | exon | 10749 | 10828 | 10749 | ID=MS.gene23953.t1.exon2;Parent=MS.gene23953.t1 |
| 15455 | CDS | 10749 | 10828 | 10749 | ID=cds.MS.gene23953.t1;Parent=MS.gene23953.t1 |
| 15455 | exon | 12025 | 12149 | 12025 | ID=MS.gene23953.t1.exon3;Parent=MS.gene23953.t1 |
| 15455 | CDS | 12025 | 12149 | 12025 | ID=cds.MS.gene23953.t1;Parent=MS.gene23953.t1 |
| Gene Sequence |
| Protein sequence |
