Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25731.t1 | AFK48023.1 | 85.2 | 162 | 18 | 2 | 1 | 162 | 1 | 156 | 1.90E-69 | 271.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25731.t1 | I3T681 | 85.2 | 162 | 18 | 2 | 1 | 162 | 1 | 156 | 1.3e-69 | 271.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051816 | MS.gene25731 | 0.81115 | 8.08E-51 | -1.69E-46 |
| MS.gene051955 | MS.gene25731 | 0.83421 | 3.42E-56 | -1.69E-46 |
| MS.gene052308 | MS.gene25731 | 0.863871 | 1.83E-64 | -1.69E-46 |
| MS.gene052367 | MS.gene25731 | 0.821066 | 4.92E-53 | -1.69E-46 |
| MS.gene053173 | MS.gene25731 | 0.808441 | 3.09E-50 | -1.69E-46 |
| MS.gene053348 | MS.gene25731 | 0.82201 | 2.98E-53 | -1.69E-46 |
| MS.gene054551 | MS.gene25731 | 0.823373 | 1.43E-53 | -1.69E-46 |
| MS.gene054593 | MS.gene25731 | 0.818364 | 2.04E-52 | -1.69E-46 |
| MS.gene055308 | MS.gene25731 | 0.832843 | 7.50E-56 | -1.69E-46 |
| MS.gene055578 | MS.gene25731 | 0.877914 | 4.29E-69 | -1.69E-46 |
| MS.gene055579 | MS.gene25731 | 0.811726 | 6.06E-51 | -1.69E-46 |
| MS.gene055957 | MS.gene25731 | 0.825938 | 3.57E-54 | -1.69E-46 |
| MS.gene056041 | MS.gene25731 | 0.869743 | 2.46E-66 | -1.69E-46 |
| MS.gene05640 | MS.gene25731 | 0.817071 | 3.99E-52 | -1.69E-46 |
| MS.gene056528 | MS.gene25731 | 0.815914 | 7.24E-52 | -1.69E-46 |
| MS.gene057458 | MS.gene25731 | 0.823409 | 1.41E-53 | -1.69E-46 |
| MS.gene058128 | MS.gene25731 | 0.844142 | 9.08E-59 | -1.69E-46 |
| MS.gene058853 | MS.gene25731 | 0.848895 | 4.58E-60 | -1.69E-46 |
| MS.gene059616 | MS.gene25731 | 0.822446 | 2.36E-53 | -1.69E-46 |
| MS.gene060473 | MS.gene25731 | 0.844261 | 8.43E-59 | -1.69E-46 |
| MS.gene060565 | MS.gene25731 | 0.821671 | 3.57E-53 | -1.69E-46 |
| MS.gene061099 | MS.gene25731 | 0.846898 | 1.63E-59 | -1.69E-46 |
| MS.gene061260 | MS.gene25731 | 0.845567 | 3.75E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25731.t1 | MTR_3g082070 | 91.975 | 162 | 8 | 2 | 1 | 162 | 1 | 157 | 2.02e-105 | 299 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25731.t1 | AT4G15140 | 75.472 | 53 | 12 | 1 | 19 | 70 | 19 | 71 | 4.48e-20 | 82.0 |
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCCTTCAATCTCAAATT+TGG | 0.164475 | 3.3:-69119411 | MS.gene25731:CDS |
| ACTACTCTCAAAGCACTATT+TGG | 0.262045 | 3.3:-69119572 | MS.gene25731:CDS |
| ACCCTATTCTCCCATGGATT+TGG | 0.270878 | 3.3:-69119532 | MS.gene25731:CDS |
| AAATCATAAGCAGAGTTATC+TGG | 0.289654 | 3.3:+69119597 | None:intergenic |
| TAACCCCATCATCAATAATA+AGG | 0.309837 | 3.3:-69119310 | MS.gene25731:intron |
| ATGTCATAGGAGAGTTTCTT+TGG | 0.315934 | 3.3:+69119498 | None:intergenic |
| ATTTGATCCTTGAAGGTGTT+GGG | 0.350471 | 3.3:+69119340 | None:intergenic |
| AAGCACTATTTGGTCCCCTT+TGG | 0.370267 | 3.3:-69119562 | MS.gene25731:CDS |
| AAATACCTTATTATTGATGA+TGG | 0.374171 | 3.3:+69119305 | None:intergenic |
| ATTTCTCCCCAACACCTTCA+AGG | 0.390688 | 3.3:-69119347 | MS.gene25731:CDS |
| TCCAAATCCATGGGAGAATA+GGG | 0.407407 | 3.3:+69119531 | None:intergenic |
| CATGCACATTTGATCCTTGA+AGG | 0.408025 | 3.3:+69119333 | None:intergenic |
| GAAACCAAATTTGAGATTGA+AGG | 0.423550 | 3.3:+69119407 | None:intergenic |
| ACAAGGTTGGTTCTAAGCTT+AGG | 0.427431 | 3.3:-69119449 | MS.gene25731:CDS |
| ATCCAAATCCATGGGAGAAT+AGG | 0.427751 | 3.3:+69119530 | None:intergenic |
| AATACCTTATTATTGATGAT+GGG | 0.459464 | 3.3:+69119306 | None:intergenic |
| CATTTGATCCTTGAAGGTGT+TGG | 0.463929 | 3.3:+69119339 | None:intergenic |
| AGCTCCTAAGCCAATGTCAT+AGG | 0.476044 | 3.3:+69119485 | None:intergenic |
| TCCATGGGAGAATAGGGTCT+TGG | 0.487632 | 3.3:+69119537 | None:intergenic |
| AATCATAAGCAGAGTTATCT+GGG | 0.497222 | 3.3:+69119598 | None:intergenic |
| GGAATTTGCAGAGAAGCTCT+AGG | 0.507179 | 3.3:+69119619 | None:intergenic |
| AATAGGGTCTTGGAACCAAA+GGG | 0.508609 | 3.3:+69119547 | None:intergenic |
| CTCTCCTATGACATTGGCTT+AGG | 0.509647 | 3.3:-69119489 | MS.gene25731:CDS |
| AAGAAACTCTCCTATGACAT+TGG | 0.513119 | 3.3:-69119495 | MS.gene25731:CDS |
| ATACCTTATTATTGATGATG+GGG | 0.543732 | 3.3:+69119307 | None:intergenic |
| TTTGTAGTGTTCAGTAACAA+TGG | 0.553446 | 3.3:+69119672 | None:intergenic |
| AGATGCAGTGTCAACAAGGT+TGG | 0.554476 | 3.3:-69119462 | MS.gene25731:CDS |
| ATGTGATACAGAGATGGGGC+AGG | 0.556775 | 3.3:-69119064 | MS.gene25731:intron |
| TTTGATCCTTGAAGGTGTTG+GGG | 0.561529 | 3.3:+69119341 | None:intergenic |
| TCCAAGACCCTATTCTCCCA+TGG | 0.565449 | 3.3:-69119538 | MS.gene25731:CDS |
| AGTAATGAGATCCAAATCCA+TGG | 0.566074 | 3.3:+69119521 | None:intergenic |
| GAATAGGGTCTTGGAACCAA+AGG | 0.567058 | 3.3:+69119546 | None:intergenic |
| CTTCAAAACAGTTCAAGAGA+TGG | 0.567072 | 3.3:-69119028 | MS.gene25731:CDS |
| AGCTAGATGCAGTGTCAACA+AGG | 0.580501 | 3.3:-69119466 | MS.gene25731:CDS |
| GAATTTGCAGAGAAGCTCTA+GGG | 0.600602 | 3.3:+69119620 | None:intergenic |
| AGAAGCTCTAGGGACATGAA+TGG | 0.605385 | 3.3:+69119630 | None:intergenic |
| AAAACAGTTCAAGAGATGGA+AGG | 0.606838 | 3.3:-69119024 | MS.gene25731:CDS |
| TGTTGTTCTTCTGCTTCGTG+TGG | 0.615192 | 3.3:+69119699 | None:intergenic |
| GTAATGAGATCCAAATCCAT+GGG | 0.641690 | 3.3:+69119522 | None:intergenic |
| TGTGATACAGAGATGGGGCA+GGG | 0.647718 | 3.3:-69119063 | MS.gene25731:intron |
| TACTATGTGATACAGAGATG+GGG | 0.699067 | 3.3:-69119068 | MS.gene25731:intron |
| ATAGGGTCTTGGAACCAAAG+GGG | 0.746078 | 3.3:+69119548 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTTAAACTCTT+TGG | - | chr3.3:69119542-69119561 | MS.gene25731:CDS | 10.0% |
| !! | AAATACCTTATTATTGATGA+TGG | + | chr3.3:69119431-69119450 | None:intergenic | 20.0% |
| !! | GATGAAATTGAAGTAATTAT+TGG | - | chr3.3:69119625-69119644 | MS.gene25731:CDS | 20.0% |
| !!! | AATACCTTATTATTGATGAT+GGG | + | chr3.3:69119430-69119449 | None:intergenic | 20.0% |
| ! | AATTCCTTCAATCTCAAATT+TGG | - | chr3.3:69119322-69119341 | MS.gene25731:CDS | 25.0% |
| !! | AATTATTGGTGATAACTTGT+GGG | - | chr3.3:69119639-69119658 | MS.gene25731:CDS | 25.0% |
| !! | CTGTTCTGTTTTATGATATT+GGG | - | chr3.3:69119501-69119520 | MS.gene25731:CDS | 25.0% |
| !! | TAATTATTGGTGATAACTTG+TGG | - | chr3.3:69119638-69119657 | MS.gene25731:CDS | 25.0% |
| !! | TCTGTTCTGTTTTATGATAT+TGG | - | chr3.3:69119500-69119519 | MS.gene25731:CDS | 25.0% |
| !!! | ATACCTTATTATTGATGATG+GGG | + | chr3.3:69119429-69119448 | None:intergenic | 25.0% |
| AAATCATAAGCAGAGTTATC+TGG | + | chr3.3:69119139-69119158 | None:intergenic | 30.0% | |
| AATCATAAGCAGAGTTATCT+GGG | + | chr3.3:69119138-69119157 | None:intergenic | 30.0% | |
| TAACCCCATCATCAATAATA+AGG | - | chr3.3:69119423-69119442 | MS.gene25731:CDS | 30.0% | |
| TTTGTAGTGTTCAGTAACAA+TGG | + | chr3.3:69119064-69119083 | None:intergenic | 30.0% | |
| ! | GAAACCAAATTTGAGATTGA+AGG | + | chr3.3:69119329-69119348 | None:intergenic | 30.0% |
| AAAACAGTTCAAGAGATGGA+AGG | - | chr3.3:69119709-69119728 | MS.gene25731:CDS | 35.0% | |
| AAGAAACTCTCCTATGACAT+TGG | - | chr3.3:69119238-69119257 | MS.gene25731:intron | 35.0% | |
| AGTAATGAGATCCAAATCCA+TGG | + | chr3.3:69119215-69119234 | None:intergenic | 35.0% | |
| ATGTCATAGGAGAGTTTCTT+TGG | + | chr3.3:69119238-69119257 | None:intergenic | 35.0% | |
| CTACTATGTGATACAGAGAT+GGG | - | chr3.3:69119664-69119683 | MS.gene25731:CDS | 35.0% | |
| CTTCAAAACAGTTCAAGAGA+TGG | - | chr3.3:69119705-69119724 | MS.gene25731:CDS | 35.0% | |
| GTAATGAGATCCAAATCCAT+GGG | + | chr3.3:69119214-69119233 | None:intergenic | 35.0% | |
| TACTATGTGATACAGAGATG+GGG | - | chr3.3:69119665-69119684 | MS.gene25731:CDS | 35.0% | |
| ! | ACTACTCTCAAAGCACTATT+TGG | - | chr3.3:69119161-69119180 | MS.gene25731:intron | 35.0% |
| !! | ATTTGATCCTTGAAGGTGTT+GGG | + | chr3.3:69119396-69119415 | None:intergenic | 35.0% |
| AATAGGGTCTTGGAACCAAA+GGG | + | chr3.3:69119189-69119208 | None:intergenic | 40.0% | |
| ATCCAAATCCATGGGAGAAT+AGG | + | chr3.3:69119206-69119225 | None:intergenic | 40.0% | |
| CATGCACATTTGATCCTTGA+AGG | + | chr3.3:69119403-69119422 | None:intergenic | 40.0% | |
| GAATTTGCAGAGAAGCTCTA+GGG | + | chr3.3:69119116-69119135 | None:intergenic | 40.0% | |
| GCTACTATGTGATACAGAGA+TGG | - | chr3.3:69119663-69119682 | MS.gene25731:CDS | 40.0% | |
| TCCAAATCCATGGGAGAATA+GGG | + | chr3.3:69119205-69119224 | None:intergenic | 40.0% | |
| ! | CATTTGATCCTTGAAGGTGT+TGG | + | chr3.3:69119397-69119416 | None:intergenic | 40.0% |
| !! | ACAAGGTTGGTTCTAAGCTT+AGG | - | chr3.3:69119284-69119303 | MS.gene25731:intron | 40.0% |
| !! | TTTGATCCTTGAAGGTGTTG+GGG | + | chr3.3:69119395-69119414 | None:intergenic | 40.0% |
| AAGCACTATTTGGTCCCCTT+TGG | - | chr3.3:69119171-69119190 | MS.gene25731:intron | 45.0% | |
| ACCCTATTCTCCCATGGATT+TGG | - | chr3.3:69119201-69119220 | MS.gene25731:intron | 45.0% | |
| AGATGCAGTGTCAACAAGGT+TGG | - | chr3.3:69119271-69119290 | MS.gene25731:intron | 45.0% | |
| AGCTAGATGCAGTGTCAACA+AGG | - | chr3.3:69119267-69119286 | MS.gene25731:intron | 45.0% | |
| AGCTCCTAAGCCAATGTCAT+AGG | + | chr3.3:69119251-69119270 | None:intergenic | 45.0% | |
| ATAGGGTCTTGGAACCAAAG+GGG | + | chr3.3:69119188-69119207 | None:intergenic | 45.0% | |
| ATTTCTCCCCAACACCTTCA+AGG | - | chr3.3:69119386-69119405 | MS.gene25731:CDS | 45.0% | |
| GAATAGGGTCTTGGAACCAA+AGG | + | chr3.3:69119190-69119209 | None:intergenic | 45.0% | |
| GGAATTTGCAGAGAAGCTCT+AGG | + | chr3.3:69119117-69119136 | None:intergenic | 45.0% | |
| TGTTGTTCTTCTGCTTCGTG+TGG | + | chr3.3:69119037-69119056 | None:intergenic | 45.0% | |
| !! | AGAAGCTCTAGGGACATGAA+TGG | + | chr3.3:69119106-69119125 | None:intergenic | 45.0% |
| !! | CTCTCCTATGACATTGGCTT+AGG | - | chr3.3:69119244-69119263 | MS.gene25731:intron | 45.0% |
| ATGTGATACAGAGATGGGGC+AGG | - | chr3.3:69119669-69119688 | MS.gene25731:CDS | 50.0% | |
| TCCAAGACCCTATTCTCCCA+TGG | - | chr3.3:69119195-69119214 | MS.gene25731:intron | 50.0% | |
| TCCATGGGAGAATAGGGTCT+TGG | + | chr3.3:69119199-69119218 | None:intergenic | 50.0% | |
| TGTGATACAGAGATGGGGCA+GGG | - | chr3.3:69119670-69119689 | MS.gene25731:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69119016 | 69119739 | 69119016 | ID=MS.gene25731 |
| chr3.3 | mRNA | 69119016 | 69119739 | 69119016 | ID=MS.gene25731.t1;Parent=MS.gene25731 |
| chr3.3 | exon | 69119311 | 69119739 | 69119311 | ID=MS.gene25731.t1.exon1;Parent=MS.gene25731.t1 |
| chr3.3 | CDS | 69119311 | 69119739 | 69119311 | ID=cds.MS.gene25731.t1;Parent=MS.gene25731.t1 |
| chr3.3 | exon | 69119016 | 69119075 | 69119016 | ID=MS.gene25731.t1.exon2;Parent=MS.gene25731.t1 |
| chr3.3 | CDS | 69119016 | 69119075 | 69119016 | ID=cds.MS.gene25731.t1;Parent=MS.gene25731.t1 |
| Gene Sequence |
| Protein sequence |
