AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25945

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25945.t1 MTR_3g078090 95.610 615 26 1 1 615 15 628 0.0 1179
MS.gene25945.t1 MTR_5g034320 52.318 604 271 4 22 614 32 629 0.0 617
MS.gene25945.t1 MTR_8g064560 50.564 621 291 8 3 614 13 626 0.0 598
MS.gene25945.t1 MTR_8g064530 49.528 636 290 12 1 614 17 643 0.0 590
MS.gene25945.t1 MTR_8g064500 61.011 277 106 1 338 614 99 373 4.14e-122 366
MS.gene25945.t1 MTR_4g133840 61.765 204 62 2 412 614 1 189 1.61e-90 278
MS.gene25945.t1 MTR_5g033340 50.230 217 106 2 400 615 166 381 4.12e-72 237
MS.gene25945.t1 MTR_5g033340 49.669 151 75 1 445 595 4 153 5.40e-44 162
MS.gene25945.t1 MTR_5g033330 50.935 214 103 2 400 612 405 617 4.75e-68 233
MS.gene25945.t1 MTR_5g033330 49.239 197 98 2 400 595 197 392 9.56e-57 202
MS.gene25945.t1 MTR_5g033330 47.027 185 96 2 412 595 1 184 1.18e-49 182
MS.gene25945.t1 MTR_5g033350 48.276 203 103 2 414 615 1 202 5.32e-67 217
MS.gene25945.t1 MTR_4g133830 42.165 351 169 9 80 418 50 378 1.06e-64 218
MS.gene25945.t1 MTR_5g033360 46.544 217 96 4 400 615 27 224 8.41e-62 204
MS.gene25945.t1 MTR_1g008530 35.602 191 116 3 426 613 37 223 1.29e-35 134
MS.gene25945.t1 MTR_1g008500 37.696 191 112 4 426 613 198 384 2.03e-35 137
MS.gene25945.t1 MTR_1g008560 35.602 191 116 3 426 613 143 329 2.99e-35 136
MS.gene25945.t1 MTR_1g008510 35.079 191 117 3 426 613 139 325 4.44e-35 135
MS.gene25945.t1 MTR_8g075930 56.322 87 38 0 516 602 1 87 2.97e-34 125
MS.gene25945.t1 MTR_1g008590 34.884 215 132 5 400 611 113 322 3.48e-34 133
MS.gene25945.t1 MTR_3g109490 37.104 221 124 6 400 612 107 320 7.13e-34 132
MS.gene25945.t1 MTR_0312s0030 34.419 215 133 5 400 611 113 322 1.17e-33 131
MS.gene25945.t1 MTR_1g007520 34.419 215 133 5 400 611 113 322 1.17e-33 131
MS.gene25945.t1 MTR_1g008470 36.449 214 129 5 400 611 89 297 1.41e-33 130
MS.gene25945.t1 MTR_1g008460 37.566 189 111 5 425 611 111 294 1.82e-31 124
MS.gene25945.t1 MTR_5g033430 55.682 88 37 2 529 615 26 112 1.02e-30 116
MS.gene25945.t1 MTR_4g069570 34.842 221 128 7 400 611 120 333 2.36e-30 122
MS.gene25945.t1 MTR_1g008580 34.091 176 109 3 442 614 1 172 2.87e-30 117
MS.gene25945.t1 MTR_1g008450 37.368 190 110 5 425 611 127 310 5.51e-30 120
MS.gene25945.t1 MTR_3g116410 34.404 218 136 5 400 612 59 274 1.16e-29 119
MS.gene25945.t1 MTR_5g033440 53.409 88 39 2 529 615 113 199 3.59e-29 115
MS.gene25945.t1 MTR_5g033440 52.273 88 40 2 529 615 26 112 2.82e-28 112
MS.gene25945.t1 MTR_3g107830 32.569 218 140 5 400 612 39 254 2.65e-28 114
MS.gene25945.t1 MTR_5g033420 54.118 85 37 2 529 612 14 97 3.88e-28 113
MS.gene25945.t1 MTR_5g033420 45.312 64 35 0 548 611 145 208 2.61e-15 75.9
MS.gene25945.t1 MTR_4g069040 30.909 220 138 6 400 611 294 507 1.22e-26 114
MS.gene25945.t1 MTR_4g069680 32.984 191 122 3 426 612 97 285 1.43e-26 110
MS.gene25945.t1 MTR_4g069550 31.532 222 136 7 400 612 247 461 2.62e-26 112
MS.gene25945.t1 MTR_5g033450 54.217 83 36 2 534 615 223 304 3.31e-26 109
MS.gene25945.t1 MTR_3g116380 32.719 217 140 4 400 612 87 301 3.51e-26 109
MS.gene25945.t1 MTR_5g033410 52.381 84 38 2 533 615 315 397 4.59e-25 108
MS.gene25945.t1 MTR_5g033410 47.692 65 32 2 534 597 181 244 8.35e-12 67.8
MS.gene25945.t1 MTR_3g116270 30.594 219 138 5 400 611 292 503 7.36e-24 105
MS.gene25945.t1 MTR_3g116320 32.589 224 109 8 400 602 474 676 2.70e-23 105
MS.gene25945.t1 MTR_4g069520 29.545 220 141 6 400 611 493 706 3.88e-23 104
MS.gene25945.t1 MTR_4g069520 29.545 220 141 6 400 611 507 720 5.65e-23 104
MS.gene25945.t1 MTR_6g086530 29.146 199 130 6 427 615 10 207 3.20e-16 78.2
MS.gene25945.t1 MTR_8g045890 30.345 145 91 5 468 611 294 429 3.15e-13 72.4
MS.gene25945.t1 MTR_8g045890 30.345 145 91 5 468 611 335 470 3.96e-13 72.4
MS.gene25945.t1 MTR_8g045890 30.345 145 91 5 468 611 372 507 5.37e-13 72.0
MS.gene25945.t1 MTR_4g068420 29.197 137 74 5 480 612 2 119 1.73e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25945.t1 AT1G23760 52.852 596 272 6 20 614 34 621 0.0 622
MS.gene25945.t1 AT1G70370 53.000 600 270 6 20 614 33 625 0.0 620
MS.gene25945.t1 AT1G70370 53.000 600 270 6 20 614 33 625 0.0 620
MS.gene25945.t1 AT1G60390 53.051 590 270 5 22 611 38 620 0.0 583
MS.gene25945.t1 AT5G25610 34.921 189 119 3 425 611 203 389 2.50e-27 114
MS.gene25945.t1 AT1G49320 31.551 187 119 4 435 613 94 279 1.20e-20 92.8

Find 126 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACAATAATAGAGTAAATA+AGG 0.192990 3.2:-69023958 None:intergenic
TACGGCACCGGTTCCGTTTC+CGG 0.209999 3.2:+69023208 MS.gene25945:CDS
TGGATTGGGACGAGCGTTTA+AGG 0.217102 3.2:+69023423 MS.gene25945:CDS
GCTGCGTCATTTACGAATTA+TGG 0.226872 3.2:+69023316 MS.gene25945:CDS
GCGTTTGGATTCAAATCTTA+TGG 0.236560 3.2:+69023403 MS.gene25945:CDS
AACGACACTTTCACTGAATA+TGG 0.249572 3.2:+69023358 MS.gene25945:CDS
GCTTCATAAACTCTCACTTT+AGG 0.266040 3.2:-69023988 None:intergenic
TACGGTTCCGGTTCAAATTC+CGG 0.266445 3.2:+69022869 MS.gene25945:CDS
CTTCATAAACTCTCACTTTA+GGG 0.270723 3.2:-69023987 None:intergenic
TTCACCGGTTACGGTGAATT+AGG 0.291344 3.2:+69023112 MS.gene25945:CDS
CACCGACGTTAGCGCGATTT+CGG 0.300794 3.2:-69023252 None:intergenic
CTCAACCTCGAGGATGAAAA+TGG 0.315748 3.2:-69023649 None:intergenic
TAAGCTTTGAAACTACTCTT+TGG 0.317297 3.2:-69023187 None:intergenic
GTGGATTGGTAAAGTGTGTT+TGG 0.328141 3.2:-69022620 None:intergenic
GTGTGGAGCAGAGGGTATTA+TGG 0.345374 3.2:-69022571 None:intergenic
TTGAGGGATTGGAAGTTTGT+TGG 0.345994 3.2:-69022459 None:intergenic
AAAATGTGAACGGGTCAAAA+AGG 0.347765 3.2:+69023857 MS.gene25945:CDS
GATAGAAGAAATTGAGGGAT+TGG 0.350407 3.2:-69022470 None:intergenic
GTTTGGAATAGGAATATTCT+TGG 0.352538 3.2:-69022376 None:intergenic
GGATTCAAATCTTATGGATT+GGG 0.380176 3.2:+69023409 MS.gene25945:CDS
TACGGTAACGATACAAATTC+TGG 0.381041 3.2:+69022995 MS.gene25945:CDS
CAATCAACATTCACCGGTTA+CGG 0.396679 3.2:+69023103 MS.gene25945:CDS
GCTACGGGTAAAACCGCGTT+TGG 0.402697 3.2:+69023388 MS.gene25945:CDS
TGGATTCAAATCTTATGGAT+TGG 0.403800 3.2:+69023408 MS.gene25945:CDS
TGGATTGGTAAAGTGTGTTT+GGG 0.405593 3.2:-69022619 None:intergenic
TGTTCCTAATTCACCGTAAC+CGG 0.405892 3.2:-69023116 None:intergenic
GAAGTTTGAGTCTGATTTGT+TGG 0.408309 3.2:-69022648 None:intergenic
TGGCGAATGAGTGAGGCTTT+AGG 0.421159 3.2:-69022419 None:intergenic
GCTTTAGATAGAAGAAATTG+AGG 0.426125 3.2:-69022476 None:intergenic
AAAAGTATCGGAACCGGAAA+CGG 0.427279 3.2:-69023221 None:intergenic
AACTTCACAAACTACGGTTC+CGG 0.437081 3.2:+69022857 MS.gene25945:CDS
GTTGTTTGTGTTTAAACCGT+TGG 0.449038 3.2:-69022747 None:intergenic
ATCGGAACCGGAAACGGAAC+CGG 0.456546 3.2:-69023215 None:intergenic
AAGAGTAGTTTCAAAGCTTA+CGG 0.459173 3.2:+69023190 MS.gene25945:CDS
TTTGTTTGAAGAGGTTTATG+AGG 0.460977 3.2:-69022529 None:intergenic
TCGAAGGAGCAGAATAAGTA+TGG 0.462522 3.2:-69022593 None:intergenic
GCGTAGTGTTGTGGTGTAAG+AGG 0.464051 3.2:-69022503 None:intergenic
TTTGGAATAGGAATATTCTT+GGG 0.464129 3.2:-69022375 None:intergenic
AGTGAGGCTTTAGGAGTGAA+AGG 0.471322 3.2:-69022410 None:intergenic
CTTTAGATAGAAGAAATTGA+GGG 0.472030 3.2:-69022475 None:intergenic
TCCGGTTCAAATTCCGGCAC+CGG 0.477147 3.2:+69022875 MS.gene25945:CDS
AATTTCGATGATAGAGAAAA+GGG 0.480729 3.2:-69023625 None:intergenic
GTGAGGCTTTAGGAGTGAAA+GGG 0.487675 3.2:-69022409 None:intergenic
TCCGGTGCCGGAATTTGAAC+CGG 0.489824 3.2:-69022876 None:intergenic
TAATTTCGATGATAGAGAAA+AGG 0.490704 3.2:-69023626 None:intergenic
AGTTTCAAAGCTTACGGCAC+CGG 0.495602 3.2:+69023196 MS.gene25945:CDS
GTTGCTAGCGGCGGAGTCGT+AGG 0.495675 3.2:-69022951 None:intergenic
GAATGTTGATTGAAGAATGT+TGG 0.499804 3.2:-69023092 None:intergenic
TTCAACAGCTACGCAGAAAA+CGG 0.500011 3.2:+69022818 MS.gene25945:CDS
TAAGTATGGTGTGGAGCAGA+GGG 0.501798 3.2:-69022579 None:intergenic
TTATGGAGAATTGATGAGAA+GGG 0.501836 3.2:-69022554 None:intergenic
GACAATAATAGAGTAAATAA+GGG 0.503977 3.2:-69023957 None:intergenic
ATTCTTCAATCAACATTCAC+CGG 0.504853 3.2:+69023097 MS.gene25945:CDS
AAAAGAAGCGTTTCACGCGC+GGG 0.508596 3.2:+69023678 MS.gene25945:CDS
AAACAACTGAAAATGTGAAC+GGG 0.509569 3.2:+69023848 MS.gene25945:CDS
TCTAAGTCAAAGATTAATCA+TGG 0.517107 3.2:+69024030 MS.gene25945:CDS
AAGCCTCACTCATTCGCCAT+TGG 0.520046 3.2:+69022423 MS.gene25945:CDS
AACACAAACTTCACAAACTA+CGG 0.525394 3.2:+69022851 MS.gene25945:CDS
TTTCCTTCCTCAACTAGAAG+TGG 0.526085 3.2:-69024178 None:intergenic
ATGAGGTTTGCGTAGTGTTG+TGG 0.527522 3.2:-69022512 None:intergenic
ACTCACAAAAGTATCGGAAC+CGG 0.528022 3.2:-69023227 None:intergenic
ATTCGTAAATGACGCAGCAC+CGG 0.528728 3.2:-69023311 None:intergenic
AAGTTATCTTTCTCAAGCTA+CGG 0.531063 3.2:+69022977 MS.gene25945:CDS
AGAGAGTCGATGTTAAAAGA+AGG 0.533741 3.2:+69023547 MS.gene25945:CDS
TCACATTGATACGTCATCGT+GGG 0.534023 3.2:+69024065 MS.gene25945:CDS
TTCAAAGCGTACAGTTTCAA+CGG 0.534195 3.2:+69023157 MS.gene25945:CDS
TAGGTGGTGAAACCGAGGTT+GGG 0.534668 3.2:-69022932 None:intergenic
ATGCGAGAGGGAACCGAGTG+TGG 0.534911 3.2:+69023744 MS.gene25945:CDS
CAGGTTTCTATAGCAACCAT+TGG 0.539976 3.2:+69022347 MS.gene25945:CDS
GAATATGGAAAAGGAGCTAC+GGG 0.539985 3.2:+69023373 MS.gene25945:CDS
ATTATGGAGAATTGATGAGA+AGG 0.541035 3.2:-69022555 None:intergenic
ACTTTCACTGAATATGGAAA+AGG 0.542424 3.2:+69023364 MS.gene25945:CDS
TTGGCGTTAGGGTCGGGACC+TGG 0.542561 3.2:+69024108 MS.gene25945:CDS
GACTATGGCTCGGCTCGACT+CGG 0.548943 3.2:+69022698 MS.gene25945:CDS
GGTTACGGTGAATTAGGAAC+AGG 0.550847 3.2:+69023118 MS.gene25945:CDS
GTTGGGACCTAATATAGAAA+CGG 0.552509 3.2:-69023815 None:intergenic
TCTCAATCATTCAACAGCTA+CGG 0.558071 3.2:+69023019 MS.gene25945:CDS
TGAATATGGAAAAGGAGCTA+CGG 0.559278 3.2:+69023372 MS.gene25945:CDS
TAAAAGAAGCGTTTCACGCG+CGG 0.568395 3.2:+69023677 MS.gene25945:CDS
TCGGTAACTCACAAAAGTAT+CGG 0.572552 3.2:-69023233 None:intergenic
GAAGCGTTTCACGCGCGGGA+TGG 0.572632 3.2:+69023682 MS.gene25945:CDS
AACAATTTCAGCACAATCAG+TGG 0.580141 3.2:+69023481 MS.gene25945:CDS
CTCGTTAATGTACCCAACCT+CGG 0.580215 3.2:+69022920 MS.gene25945:CDS
TACGGTGCAAAATCGAAAGC+CGG 0.581511 3.2:+69023292 MS.gene25945:CDS
GGAGAAACAAAACGGTGCGT+TGG 0.581963 3.2:+69023766 MS.gene25945:CDS
GATTGGAAAAGTCGAAGCAA+TGG 0.587728 3.2:+69023888 MS.gene25945:CDS
GATGATTCGTTTCAAAGCTA+CGG 0.589219 3.2:+69023274 MS.gene25945:CDS
ATAAGTATGGTGTGGAGCAG+AGG 0.589833 3.2:-69022580 None:intergenic
AAAACAACTGAAAATGTGAA+CGG 0.591603 3.2:+69023847 MS.gene25945:CDS
TCATCGTGGGGTCCTAAACA+TGG 0.594368 3.2:+69024078 MS.gene25945:CDS
AAGTGTGTTTGGGTGGTCGA+AGG 0.601561 3.2:-69022609 None:intergenic
TGAAGCTGATATTCTTGACG+TGG 0.604319 3.2:+69024005 MS.gene25945:CDS
AAAATTCTCAGACTATGGCT+CGG 0.607659 3.2:+69022688 MS.gene25945:CDS
TAACTTGTGGTTGCTAGCGG+CGG 0.607719 3.2:-69022960 None:intergenic
CGGTGCGTTGGATCAGCTGA+GGG 0.613492 3.2:+69023778 MS.gene25945:CDS
CGAGTGTGGGAGAAACAAAA+CGG 0.616396 3.2:+69023758 MS.gene25945:CDS
TGACGTATCAATGTGACAGA+TGG 0.618564 3.2:-69024058 None:intergenic
GCTAGCGGCGGAGTCGTAGG+TGG 0.618692 3.2:-69022948 None:intergenic
TGCGAGAGGGAACCGAGTGT+GGG 0.618918 3.2:+69023745 MS.gene25945:CDS
GTAGGTGGTGAAACCGAGGT+TGG 0.619572 3.2:-69022933 None:intergenic
TACCGAAATCGCGCTAACGT+CGG 0.620564 3.2:+69023250 MS.gene25945:CDS
TATGGACAATCATTTAACGA+AGG 0.621102 3.2:+69023334 MS.gene25945:CDS
GTAAATAGATTTGTAGAACC+AGG 0.625185 3.2:+69023514 MS.gene25945:CDS
ACGTGGACCACTTCTAGTTG+AGG 0.629765 3.2:+69024171 MS.gene25945:CDS
CACATTGATACGTCATCGTG+GGG 0.632982 3.2:+69024066 MS.gene25945:CDS
ACGGTGCGTTGGATCAGCTG+AGG 0.635487 3.2:+69023777 MS.gene25945:CDS
GTCACATTGATACGTCATCG+TGG 0.637640 3.2:+69024064 MS.gene25945:CDS
GGAGCAGAATAAGTATGGTG+TGG 0.637921 3.2:-69022588 None:intergenic
AGCTTGAGAAAGATAACTTG+TGG 0.639570 3.2:-69022973 None:intergenic
AGATAACTTGTGGTTGCTAG+CGG 0.643189 3.2:-69022963 None:intergenic
CGCGCTTAAAGAATGCGAGA+GGG 0.646475 3.2:+69023732 MS.gene25945:CDS
TTAGAGATAAAATGCCTGCA+AGG 0.646765 3.2:+69023593 MS.gene25945:CDS
TACGGTGAATTAGGAACAGG+AGG 0.647425 3.2:+69023121 MS.gene25945:CDS
TCAGGCTCAACTCGTCGCGG+TGG 0.649482 3.2:+69022791 MS.gene25945:CDS
ACGCGCTTAAAGAATGCGAG+AGG 0.653096 3.2:+69023731 MS.gene25945:CDS
GTTCCAATGGCGAATGAGTG+AGG 0.654041 3.2:-69022426 None:intergenic
ATTGGTAAAGTGTGTTTGGG+TGG 0.654549 3.2:-69022616 None:intergenic
TATATTAGGTCCCAACGTGG+TGG 0.658022 3.2:+69023822 MS.gene25945:CDS
TATGGCTCGGCTCGACTCGG+CGG 0.661211 3.2:+69022701 MS.gene25945:CDS
GGAAAAGTCGAAGCAATGGA+CGG 0.666675 3.2:+69023892 MS.gene25945:CDS
TTCTATATTAGGTCCCAACG+TGG 0.667872 3.2:+69023819 MS.gene25945:CDS
AGTCGTAGGTGGTGAAACCG+AGG 0.675434 3.2:-69022937 None:intergenic
TACGGAAAACGCGTTCGTAG+CGG 0.678553 3.2:+69023037 MS.gene25945:CDS
AGCTCAGGCTCAACTCGTCG+CGG 0.679160 3.2:+69022788 MS.gene25945:CDS
AAAGTCGAAGCAATGGACGG+TGG 0.689713 3.2:+69023895 MS.gene25945:CDS
ATAAGATTTGAATCCAAACG+CGG 0.725665 3.2:-69023401 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GACAATAATAGAGTAAATAA+GGG - chr3.2:69023960-69023979 None:intergenic 20.0%
!! TGACAATAATAGAGTAAATA+AGG - chr3.2:69023961-69023980 None:intergenic 20.0%
!!! TTTAAGGTATACAACAAAAA+CGG + chr3.2:69023439-69023458 MS.gene25945:CDS 20.0%
! AAAACAACTGAAAATGTGAA+CGG + chr3.2:69023847-69023866 MS.gene25945:CDS 25.0%
! AACAAAAAATTCTCAGACTA+TGG + chr3.2:69022683-69022702 MS.gene25945:CDS 25.0%
! AATTTCGATGATAGAGAAAA+GGG - chr3.2:69023628-69023647 None:intergenic 25.0%
! CTTTAGATAGAAGAAATTGA+GGG - chr3.2:69022478-69022497 None:intergenic 25.0%
! TAATTTCGATGATAGAGAAA+AGG - chr3.2:69023629-69023648 None:intergenic 25.0%
! TCTAAGTCAAAGATTAATCA+TGG + chr3.2:69024030-69024049 MS.gene25945:CDS 25.0%
!! TTTGGAATAGGAATATTCTT+GGG - chr3.2:69022378-69022397 None:intergenic 25.0%
!!! GAATTTTTTGTTTGAGTAGA+CGG - chr3.2:69022675-69022694 None:intergenic 25.0%
AAACAACTGAAAATGTGAAC+GGG + chr3.2:69023848-69023867 MS.gene25945:CDS 30.0%
AACACAAACTTCACAAACTA+CGG + chr3.2:69022851-69022870 MS.gene25945:CDS 30.0%
AAGAGTAGTTTCAAAGCTTA+CGG + chr3.2:69023190-69023209 MS.gene25945:CDS 30.0%
AAGTTATCTTTCTCAAGCTA+CGG + chr3.2:69022977-69022996 MS.gene25945:CDS 30.0%
ACTTTCACTGAATATGGAAA+AGG + chr3.2:69023364-69023383 MS.gene25945:CDS 30.0%
ATAAGATTTGAATCCAAACG+CGG - chr3.2:69023404-69023423 None:intergenic 30.0%
ATTATGGAGAATTGATGAGA+AGG - chr3.2:69022558-69022577 None:intergenic 30.0%
ATTCTTCAATCAACATTCAC+CGG + chr3.2:69023097-69023116 MS.gene25945:CDS 30.0%
CTTCATAAACTCTCACTTTA+GGG - chr3.2:69023990-69024009 None:intergenic 30.0%
GGATTCAAATCTTATGGATT+GGG + chr3.2:69023409-69023428 MS.gene25945:CDS 30.0%
GTAAATAGATTTGTAGAACC+AGG + chr3.2:69023514-69023533 MS.gene25945:CDS 30.0%
TATGGACAATCATTTAACGA+AGG + chr3.2:69023334-69023353 MS.gene25945:CDS 30.0%
TCATTCAAAAACTACTCCAA+CGG + chr3.2:69022731-69022750 MS.gene25945:CDS 30.0%
TGGATTCAAATCTTATGGAT+TGG + chr3.2:69023408-69023427 MS.gene25945:CDS 30.0%
TTATGGAGAATTGATGAGAA+GGG - chr3.2:69022557-69022576 None:intergenic 30.0%
TTTGTTTGAAGAGGTTTATG+AGG - chr3.2:69022532-69022551 None:intergenic 30.0%
! GAATGTTGATTGAAGAATGT+TGG - chr3.2:69023095-69023114 None:intergenic 30.0%
! GACTTTTCCGTTTCTATATT+AGG + chr3.2:69023808-69023827 MS.gene25945:CDS 30.0%
! GCTTTAGATAGAAGAAATTG+AGG - chr3.2:69022479-69022498 None:intergenic 30.0%
! GGCATTTTATCTCTAATATC+TGG - chr3.2:69023589-69023608 None:intergenic 30.0%
! GTTTGGAATAGGAATATTCT+TGG - chr3.2:69022379-69022398 None:intergenic 30.0%
!! GTTTGTCTTTTGTTTGGAAT+AGG - chr3.2:69022390-69022409 None:intergenic 30.0%
!! TAAGCTTTGAAACTACTCTT+TGG - chr3.2:69023190-69023209 None:intergenic 30.0%
!! TTTTTGAGAATGATATGACG+TGG + chr3.2:69024154-69024173 MS.gene25945:CDS 30.0%
!!! ATTTGTTGGTTTTTGTGGAT+TGG - chr3.2:69022637-69022656 None:intergenic 30.0%
AAAATGTGAACGGGTCAAAA+AGG + chr3.2:69023857-69023876 MS.gene25945:CDS 35.0%
AAAATTCTCAGACTATGGCT+CGG + chr3.2:69022688-69022707 MS.gene25945:CDS 35.0%
AACAATTTCAGCACAATCAG+TGG + chr3.2:69023481-69023500 MS.gene25945:CDS 35.0%
AACGACACTTTCACTGAATA+TGG + chr3.2:69023358-69023377 MS.gene25945:CDS 35.0%
AGAGAGTCGATGTTAAAAGA+AGG + chr3.2:69023547-69023566 MS.gene25945:CDS 35.0%
AGCTTGAGAAAGATAACTTG+TGG - chr3.2:69022976-69022995 None:intergenic 35.0%
GAAAAATTGAGGTGTGTCAT+TGG + chr3.2:69024130-69024149 MS.gene25945:CDS 35.0%
GATAGAAGAAATTGAGGGAT+TGG - chr3.2:69022473-69022492 None:intergenic 35.0%
GATGATTCGTTTCAAAGCTA+CGG + chr3.2:69023274-69023293 MS.gene25945:CDS 35.0%
GCGTTTGGATTCAAATCTTA+TGG + chr3.2:69023403-69023422 MS.gene25945:CDS 35.0%
GCTTCATAAACTCTCACTTT+AGG - chr3.2:69023991-69024010 None:intergenic 35.0%
GTTGGGACCTAATATAGAAA+CGG - chr3.2:69023818-69023837 None:intergenic 35.0%
GTTGTTTGTGTTTAAACCGT+TGG - chr3.2:69022750-69022769 None:intergenic 35.0%
TACGGTAACGATACAAATTC+TGG + chr3.2:69022995-69023014 MS.gene25945:CDS 35.0%
TCGTTCAAAAAATACAGCTC+AGG + chr3.2:69022773-69022792 MS.gene25945:CDS 35.0%
TCTCAATCATTCAACAGCTA+CGG + chr3.2:69023019-69023038 MS.gene25945:CDS 35.0%
TGAATATGGAAAAGGAGCTA+CGG + chr3.2:69023372-69023391 MS.gene25945:CDS 35.0%
TTAGAGATAAAATGCCTGCA+AGG + chr3.2:69023593-69023612 MS.gene25945:CDS 35.0%
TTCAAAGCGTACAGTTTCAA+CGG + chr3.2:69023157-69023176 MS.gene25945:CDS 35.0%
! AATTACCATTTTCATCCTCG+AGG + chr3.2:69023644-69023663 MS.gene25945:CDS 35.0%
! ATATTCTTGGGTTTTGCCAA+TGG - chr3.2:69022366-69022385 None:intergenic 35.0%
! GAAAGGGTTTGTCTTTTGTT+TGG - chr3.2:69022396-69022415 None:intergenic 35.0%
! GAAGTTTGAGTCTGATTTGT+TGG - chr3.2:69022651-69022670 None:intergenic 35.0%
! GACTCTCTGAAAAATTTACC+TGG - chr3.2:69023535-69023554 None:intergenic 35.0%
! TCGGTAACTCACAAAAGTAT+CGG - chr3.2:69023236-69023255 None:intergenic 35.0%
! TGGAGATTTTTGTGTTCCAA+TGG - chr3.2:69022442-69022461 None:intergenic 35.0%
!! AGAAGGGTTTTTGTTTGAAG+AGG - chr3.2:69022541-69022560 None:intergenic 35.0%
!! TGGATTGGTAAAGTGTGTTT+GGG - chr3.2:69022622-69022641 None:intergenic 35.0%
!!! GTCTGATTTGTTGGTTTTTG+TGG - chr3.2:69022642-69022661 None:intergenic 35.0%
!!! TCCTAAACATGGTGCTTTTT+TGG + chr3.2:69024089-69024108 MS.gene25945:CDS 35.0%
AACTTCACAAACTACGGTTC+CGG + chr3.2:69022857-69022876 MS.gene25945:CDS 40.0%
ATTGGTAAAGTGTGTTTGGG+TGG - chr3.2:69022619-69022638 None:intergenic 40.0%
CAGGTTTCTATAGCAACCAT+TGG + chr3.2:69022347-69022366 MS.gene25945:CDS 40.0%
GAATATGGAAAAGGAGCTAC+GGG + chr3.2:69023373-69023392 MS.gene25945:CDS 40.0%
GATTGGAAAAGTCGAAGCAA+TGG + chr3.2:69023888-69023907 MS.gene25945:CDS 40.0%
GCTGCGTCATTTACGAATTA+TGG + chr3.2:69023316-69023335 MS.gene25945:CDS 40.0%
TCAAAAAGGACGGTGATGAT+TGG + chr3.2:69023871-69023890 MS.gene25945:CDS 40.0%
TCACATTGATACGTCATCGT+GGG + chr3.2:69024065-69024084 MS.gene25945:CDS 40.0%
TCGAAGGAGCAGAATAAGTA+TGG - chr3.2:69022596-69022615 None:intergenic 40.0%
TGAAGCTGATATTCTTGACG+TGG + chr3.2:69024005-69024024 MS.gene25945:CDS 40.0%
TGACGTATCAATGTGACAGA+TGG - chr3.2:69024061-69024080 None:intergenic 40.0%
TGTTCCTAATTCACCGTAAC+CGG - chr3.2:69023119-69023138 None:intergenic 40.0%
TTCAACAGCTACGCAGAAAA+CGG + chr3.2:69022818-69022837 MS.gene25945:CDS 40.0%
TTCTATATTAGGTCCCAACG+TGG + chr3.2:69023819-69023838 MS.gene25945:CDS 40.0%
TTGAGGGATTGGAAGTTTGT+TGG - chr3.2:69022462-69022481 None:intergenic 40.0%
! AGATAACTTGTGGTTGCTAG+CGG - chr3.2:69022966-69022985 None:intergenic 40.0%
! CAATCAACATTCACCGGTTA+CGG + chr3.2:69023103-69023122 MS.gene25945:CDS 40.0%
! TGACACACCTCAATTTTTCC+AGG - chr3.2:69024129-69024148 None:intergenic 40.0%
! TTTTATCTCACTCAACCTCG+AGG - chr3.2:69023662-69023681 None:intergenic 40.0%
!! AAAAGTATCGGAACCGGAAA+CGG - chr3.2:69023224-69023243 None:intergenic 40.0%
!! ACTCACAAAAGTATCGGAAC+CGG - chr3.2:69023230-69023249 None:intergenic 40.0%
!! GCCAAAAAAGCACCATGTTT+AGG - chr3.2:69024093-69024112 None:intergenic 40.0%
!! GTGGATTGGTAAAGTGTGTT+TGG - chr3.2:69022623-69022642 None:intergenic 40.0%
!!! ATCGTAGCTTTTGAAGTCTC+CGG - chr3.2:69022897-69022916 None:intergenic 40.0%
!!! ATGGTGCTTTTTTGGCGTTA+GGG + chr3.2:69024097-69024116 MS.gene25945:CDS 40.0%
AAGGGTAAAAACGACCTTGC+AGG - chr3.2:69023610-69023629 None:intergenic 45.0%
AGTTTCAAAGCTTACGGCAC+CGG + chr3.2:69023196-69023215 MS.gene25945:CDS 45.0%
ATAAGTATGGTGTGGAGCAG+AGG - chr3.2:69022583-69022602 None:intergenic 45.0%
ATGAGGTTTGCGTAGTGTTG+TGG - chr3.2:69022515-69022534 None:intergenic 45.0%
ATTCGTAAATGACGCAGCAC+CGG - chr3.2:69023314-69023333 None:intergenic 45.0%
CACATTGATACGTCATCGTG+GGG + chr3.2:69024066-69024085 MS.gene25945:CDS 45.0%
CGAGTGTGGGAGAAACAAAA+CGG + chr3.2:69023758-69023777 MS.gene25945:CDS 45.0%
CTCAACCTCGAGGATGAAAA+TGG - chr3.2:69023652-69023671 None:intergenic 45.0%
CTCGTTAATGTACCCAACCT+CGG + chr3.2:69022920-69022939 MS.gene25945:CDS 45.0%
GGAAAAGTCGAAGCAATGGA+CGG + chr3.2:69023892-69023911 MS.gene25945:CDS 45.0%
GGAGCAGAATAAGTATGGTG+TGG - chr3.2:69022591-69022610 None:intergenic 45.0%
GGTTACGGTGAATTAGGAAC+AGG + chr3.2:69023118-69023137 MS.gene25945:CDS 45.0%
GTCACATTGATACGTCATCG+TGG + chr3.2:69024064-69024083 MS.gene25945:CDS 45.0%
TAAGTATGGTGTGGAGCAGA+GGG - chr3.2:69022582-69022601 None:intergenic 45.0%
TACGGTGAATTAGGAACAGG+AGG + chr3.2:69023121-69023140 MS.gene25945:CDS 45.0%
TACGGTGCAAAATCGAAAGC+CGG + chr3.2:69023292-69023311 MS.gene25945:CDS 45.0%
TACGGTTCCGGTTCAAATTC+CGG + chr3.2:69022869-69022888 MS.gene25945:CDS 45.0%
TATATTAGGTCCCAACGTGG+TGG + chr3.2:69023822-69023841 MS.gene25945:CDS 45.0%
TGTGAACGGGTCAAAAAGGA+CGG + chr3.2:69023861-69023880 MS.gene25945:CDS 45.0%
! TTCACCGGTTACGGTGAATT+AGG + chr3.2:69023112-69023131 MS.gene25945:CDS 45.0%
!! AGTGAGGCTTTAGGAGTGAA+AGG - chr3.2:69022413-69022432 None:intergenic 45.0%
!! AGTTGTTTTCACCACCACGT+TGG - chr3.2:69023836-69023855 None:intergenic 45.0%
!! GTGAGGCTTTAGGAGTGAAA+GGG - chr3.2:69022412-69022431 None:intergenic 45.0%
!! GTTGTTTTCACCACCACGTT+GGG - chr3.2:69023835-69023854 None:intergenic 45.0%
!! TAAAAGAAGCGTTTCACGCG+CGG + chr3.2:69023677-69023696 MS.gene25945:CDS 45.0%
!!! CATGGTGCTTTTTTGGCGTT+AGG + chr3.2:69024096-69024115 MS.gene25945:CDS 45.0%
!!! CGTTTTGTTTCTCCCACACT+CGG - chr3.2:69023760-69023779 None:intergenic 45.0%
!!! TGCTTTTTTGGCGTTAGGGT+CGG + chr3.2:69024101-69024120 MS.gene25945:CDS 45.0%
AAAGTCGAAGCAATGGACGG+TGG + chr3.2:69023895-69023914 MS.gene25945:CDS 50.0%
AAGCCTCACTCATTCGCCAT+TGG + chr3.2:69022423-69022442 MS.gene25945:CDS 50.0%
AAGTGTGTTTGGGTGGTCGA+AGG - chr3.2:69022612-69022631 None:intergenic 50.0%
ACGCGCTTAAAGAATGCGAG+AGG + chr3.2:69023731-69023750 MS.gene25945:CDS 50.0%
CGCGCTTAAAGAATGCGAGA+GGG + chr3.2:69023732-69023751 MS.gene25945:CDS 50.0%
GCGTAGTGTTGTGGTGTAAG+AGG - chr3.2:69022506-69022525 None:intergenic 50.0%
GGAGAAACAAAACGGTGCGT+TGG + chr3.2:69023766-69023785 MS.gene25945:CDS 50.0%
GTCGGGACCTGGAAAAATTG+AGG + chr3.2:69024119-69024138 MS.gene25945:CDS 50.0%
GTTCCAATGGCGAATGAGTG+AGG - chr3.2:69022429-69022448 None:intergenic 50.0%
TACCGAAATCGCGCTAACGT+CGG + chr3.2:69023250-69023269 MS.gene25945:CDS 50.0%
TACGGAAAACGCGTTCGTAG+CGG + chr3.2:69023037-69023056 MS.gene25945:CDS 50.0%
TAGGTGGTGAAACCGAGGTT+GGG - chr3.2:69022935-69022954 None:intergenic 50.0%
TCATCGTGGGGTCCTAAACA+TGG + chr3.2:69024078-69024097 MS.gene25945:CDS 50.0%
TGGCGAATGAGTGAGGCTTT+AGG - chr3.2:69022422-69022441 None:intergenic 50.0%
! TAACTTGTGGTTGCTAGCGG+CGG - chr3.2:69022963-69022982 None:intergenic 50.0%
! TGGATTGGGACGAGCGTTTA+AGG + chr3.2:69023423-69023442 MS.gene25945:CDS 50.0%
!! AAAAGAAGCGTTTCACGCGC+GGG + chr3.2:69023678-69023697 MS.gene25945:CDS 50.0%
!! GTGTGGAGCAGAGGGTATTA+TGG - chr3.2:69022574-69022593 None:intergenic 50.0%
!!! GCTTTTTTGGCGTTAGGGTC+GGG + chr3.2:69024102-69024121 MS.gene25945:CDS 50.0%
AGTCGTAGGTGGTGAAACCG+AGG - chr3.2:69022940-69022959 None:intergenic 55.0%
ATCGGAACCGGAAACGGAAC+CGG - chr3.2:69023218-69023237 None:intergenic 55.0%
CACCGACGTTAGCGCGATTT+CGG - chr3.2:69023255-69023274 None:intergenic 55.0%
GCTACGGGTAAAACCGCGTT+TGG + chr3.2:69023388-69023407 MS.gene25945:CDS 55.0%
GTAGGTGGTGAAACCGAGGT+TGG - chr3.2:69022936-69022955 None:intergenic 55.0%
TCCGGTTCAAATTCCGGCAC+CGG + chr3.2:69022875-69022894 MS.gene25945:CDS 55.0%
! TCCGGTGCCGGAATTTGAAC+CGG - chr3.2:69022879-69022898 None:intergenic 55.0%
!!! GCTTTTGAAGTCTCCGGTGC+CGG - chr3.2:69022891-69022910 None:intergenic 55.0%
ACGGTGCGTTGGATCAGCTG+AGG + chr3.2:69023777-69023796 MS.gene25945:CDS 60.0%
AGCTCAGGCTCAACTCGTCG+CGG + chr3.2:69022788-69022807 MS.gene25945:CDS 60.0%
ATGCGAGAGGGAACCGAGTG+TGG + chr3.2:69023744-69023763 MS.gene25945:CDS 60.0%
CGGTGCGTTGGATCAGCTGA+GGG + chr3.2:69023778-69023797 MS.gene25945:CDS 60.0%
TGCGAGAGGGAACCGAGTGT+GGG + chr3.2:69023745-69023764 MS.gene25945:CDS 60.0%
! GACTATGGCTCGGCTCGACT+CGG + chr3.2:69022698-69022717 MS.gene25945:CDS 60.0%
!! TACGGCACCGGTTCCGTTTC+CGG + chr3.2:69023208-69023227 MS.gene25945:CDS 60.0%
GTTGCTAGCGGCGGAGTCGT+AGG - chr3.2:69022954-69022973 None:intergenic 65.0%
TCAGGCTCAACTCGTCGCGG+TGG + chr3.2:69022791-69022810 MS.gene25945:CDS 65.0%
TTGGCGTTAGGGTCGGGACC+TGG + chr3.2:69024108-69024127 MS.gene25945:CDS 65.0%
! GAAGCGTTTCACGCGCGGGA+TGG + chr3.2:69023682-69023701 MS.gene25945:CDS 65.0%
!! TATGGCTCGGCTCGACTCGG+CGG + chr3.2:69022701-69022720 MS.gene25945:CDS 65.0%
GCTAGCGGCGGAGTCGTAGG+TGG - chr3.2:69022951-69022970 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69022344 69024191 69022344 ID=MS.gene25945
chr3.2 mRNA 69022344 69024191 69022344 ID=MS.gene25945.t1;Parent=MS.gene25945
chr3.2 exon 69022344 69024191 69022344 ID=MS.gene25945.t1.exon1;Parent=MS.gene25945.t1
chr3.2 CDS 69022344 69024191 69022344 ID=cds.MS.gene25945.t1;Parent=MS.gene25945.t1
Gene Sequence

>MS.gene25945

ATGCAGGTTTCTATAGCAACCATTGGCAAAACCCAAGAATATTCCTATTCCAAACAAAAGACAAACCCTTTCACTCCTAAAGCCTCACTCATTCGCCATTGGAACACAAAAATCTCCAACAAACTTCCAATCCCTCAATTTCTTCTATCTAAAGCTTCTCCTCTTACACCACAACACTACGCAAACCTCATAAACCTCTTCAAACAAAAACCCTTCTCATCAATTCTCCATAATACCCTCTGCTCCACACCATACTTATTCTGCTCCTTCGACCACCCAAACACACTTTACCAATCCACAAAAACCAACAAATCAGACTCAAACTTCGCCGTCTACTCAAACAAAAAATTCTCAGACTATGGCTCGGCTCGACTCGGCGGAGTTGACTCATTCAAAAACTACTCCAACGGTTTAAACACAAACAACGACTCGTTCAAAAAATACAGCTCAGGCTCAACTCGTCGCGGTGGAGAATTCAACAGCTACGCAGAAAACGGCAATGTAGCTAACACAAACTTCACAAACTACGGTTCCGGTTCAAATTCCGGCACCGGAGACTTCAAAAGCTACGATAAACTCGTTAATGTACCCAACCTCGGTTTCACCACCTACGACTCCGCCGCTAGCAACCACAAGTTATCTTTCTCAAGCTACGGTAACGATACAAATTCTGGATCTCAATCATTCAACAGCTACGGAAAACGCGTTCGTAGCGGTAGCAGCGATTTCATCAATTATGCAGTTAGTTCCAACATTCTTCAATCAACATTCACCGGTTACGGTGAATTAGGAACAGGAGGTGCAAACGATTCTTTCAAAGCGTACAGTTTCAACGGTAATAATCCAAAGAGTAGTTTCAAAGCTTACGGCACCGGTTCCGTTTCCGGTTCCGATACTTTTGTGAGTTACCGAAATCGCGCTAACGTCGGTGATGATTCGTTTCAAAGCTACGGTGCAAAATCGAAAGCCGGTGCTGCGTCATTTACGAATTATGGACAATCATTTAACGAAGGAAACGACACTTTCACTGAATATGGAAAAGGAGCTACGGGTAAAACCGCGTTTGGATTCAAATCTTATGGATTGGGACGAGCGTTTAAGGTATACAACAAAAACGGCGCGTCGTTTTCAGCGTATAACAATTTCAGCACAATCAGTGGCAAAAAAATCGTAAATAGATTTGTAGAACCAGGTAAATTTTTCAGAGAGTCGATGTTAAAAGAAGGAAATGTGATGATTATGCCAGATATTAGAGATAAAATGCCTGCAAGGTCGTTTTTACCCTTTTCTCTATCATCGAAATTACCATTTTCATCCTCGAGGTTGAGTGAGATAAAAGAAGCGTTTCACGCGCGGGATGGTTCAGCGACAGAGCGCGTGATAGTTAACGCGCTTAAAGAATGCGAGAGGGAACCGAGTGTGGGAGAAACAAAACGGTGCGTTGGATCAGCTGAGGGTATGATTGACTTTTCCGTTTCTATATTAGGTCCCAACGTGGTGGTGAAAACAACTGAAAATGTGAACGGGTCAAAAAGGACGGTGATGATTGGAAAAGTCGAAGCAATGGACGGTGGAAAAGTGATTAAATCAGTTTCATGTCATCAGAGTTTGTACCCTTATTTACTCTATTATTGTCACTCTGTCCCTAAAGTGAGAGTTTATGAAGCTGATATTCTTGACGTGGACTCTAAGTCAAAGATTAATCATGGTGTTGCCATCTGTCACATTGATACGTCATCGTGGGGTCCTAAACATGGTGCTTTTTTGGCGTTAGGGTCGGGACCTGGAAAAATTGAGGTGTGTCATTGGATTTTTGAGAATGATATGACGTGGACCACTTCTAGTTGA
Protein sequence

>MS.gene25945.t1

MQVSIATIGKTQEYSYSKQKTNPFTPKASLIRHWNTKISNKLPIPQFLLSKASPLTPQHYANLINLFKQKPFSSILHNTLCSTPYLFCSFDHPNTLYQSTKTNKSDSNFAVYSNKKFSDYGSARLGGVDSFKNYSNGLNTNNDSFKKYSSGSTRRGGEFNSYAENGNVANTNFTNYGSGSNSGTGDFKSYDKLVNVPNLGFTTYDSAASNHKLSFSSYGNDTNSGSQSFNSYGKRVRSGSSDFINYAVSSNILQSTFTGYGELGTGGANDSFKAYSFNGNNPKSSFKAYGTGSVSGSDTFVSYRNRANVGDDSFQSYGAKSKAGAASFTNYGQSFNEGNDTFTEYGKGATGKTAFGFKSYGLGRAFKVYNKNGASFSAYNNFSTISGKKIVNRFVEPGKFFRESMLKEGNVMIMPDIRDKMPARSFLPFSLSSKLPFSSSRLSEIKEAFHARDGSATERVIVNALKECEREPSVGETKRCVGSAEGMIDFSVSILGPNVVVKTTENVNGSKRTVMIGKVEAMDGGKVIKSVSCHQSLYPYLLYYCHSVPKVRVYEADILDVDSKSKINHGVAICHIDTSSWGPKHGAFLALGSGPGKIEVCHWIFENDMTWTTSS