AlfalfaGEDB | MS.gene27495

M. sativa cultivar XinJiangDaYe / MS.gene27495

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27495.t1	XP_013449250.1	93.5	168	11	0	1	168	1	168	1.10E-75	292.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27495.t1	A0A072U0R7	93.5	168	11	0	1	168	1	168	7.6e-76	292.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049989	MS.gene27495	0.821922	3.12E-53	-1.69E-46
MS.gene050297	MS.gene27495	0.813707	2.23E-51	-1.69E-46
MS.gene050338	MS.gene27495	0.80238	5.77E-49	-1.69E-46
MS.gene051742	MS.gene27495	0.825576	4.35E-54	-1.69E-46
MS.gene051955	MS.gene27495	0.806038	9.99E-50	-1.69E-46
MS.gene052143	MS.gene27495	0.85141	9.04E-61	-1.69E-46
MS.gene052308	MS.gene27495	0.842517	2.46E-58	-1.69E-46
MS.gene052367	MS.gene27495	0.883175	5.60E-71	-1.69E-46
MS.gene053173	MS.gene27495	0.835485	1.63E-56	-1.69E-46
MS.gene053416	MS.gene27495	0.832154	1.11E-55	-1.69E-46
MS.gene053417	MS.gene27495	0.835485	1.63E-56	-1.69E-46
MS.gene054551	MS.gene27495	0.826361	2.83E-54	-1.69E-46
MS.gene054593	MS.gene27495	0.81615	6.41E-52	-1.69E-46
MS.gene055308	MS.gene27495	0.835431	1.69E-56	-1.69E-46
MS.gene055578	MS.gene27495	0.841618	4.26E-58	-1.69E-46
MS.gene055957	MS.gene27495	0.821274	4.41E-53	-1.69E-46
MS.gene056041	MS.gene27495	0.857269	1.83E-62	-1.69E-46
MS.gene056266	MS.gene27495	-0.800191	1.62E-48	-1.69E-46
MS.gene05640	MS.gene27495	0.804926	1.71E-49	-1.69E-46
MS.gene057458	MS.gene27495	0.810777	9.74E-51	-1.69E-46
MS.gene057483	MS.gene27495	0.829561	4.80E-55	-1.69E-46
MS.gene058853	MS.gene27495	0.824317	8.62E-54	-1.69E-46
MS.gene060473	MS.gene27495	0.821112	4.80E-53	-1.69E-46
MS.gene060565	MS.gene27495	0.814833	1.26E-51	-1.69E-46
MS.gene06057	MS.gene27495	0.815901	7.29E-52	-1.69E-46
MS.gene06093	MS.gene27495	0.809247	2.08E-50	-1.69E-46
MS.gene061099	MS.gene27495	0.822693	2.07E-53	-1.69E-46
MS.gene061260	MS.gene27495	0.841325	5.09E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27495.t1	MTR_7g073500	93.452	168	11	0	1	168	1	168	1.21e-109	311

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27495.t1	AT5G01910	46.715	137	61	2	19	155	4	128	1.63e-29	106
MS.gene27495.t1	AT5G01910	46.715	137	61	2	19	155	4	128	3.76e-29	105

Find 37 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGATCGGCGGGAGTGTTTA+TGG	0.245932	7.2:-31180595	None:intergenic
CGATAGATTGTTCCGTTTGA+AGG	0.364571	7.2:+31180899	MS.gene27495:CDS
TAATTGATCGAGCTTGGGTT+TGG	0.365621	7.2:-31180640	None:intergenic
AAAACATAATTGATCGAGCT+TGG	0.395643	7.2:-31180646	None:intergenic
AATTGATCGAGCTTGGGTTT+GGG	0.402211	7.2:-31180639	None:intergenic
GTGTCCGGCCAGAGCGTGGC+AGG	0.407635	7.2:-31180563	None:intergenic
GAGCTTGGGTTTGGGCTTTG+TGG	0.415984	7.2:-31180631	None:intergenic
CCGAAAATCCTGCCACGCTC+TGG	0.419711	7.2:+31180555	MS.gene27495:CDS
AAACATAATTGATCGAGCTT+GGG	0.445986	7.2:-31180645	None:intergenic
GTTTGGGCTTTGTGGAAAGA+TGG	0.456431	7.2:-31180623	None:intergenic
AGATCGGCGGGAGTGTTTAT+GGG	0.461473	7.2:-31180594	None:intergenic
CGCCACCGTGACTTCTCCAC+TGG	0.478503	7.2:+31180707	MS.gene27495:CDS
TATTCGAACCCTTAGAGAAA+AGG	0.487625	7.2:+31181037	MS.gene27495:CDS
GTTGCTTGACGCGAAGATGA+AGG	0.492065	7.2:+31180830	MS.gene27495:CDS
GAAGTCACGGTGGCGGTGAT+CGG	0.499844	7.2:-31180699	None:intergenic
AAATCCTGCCACGCTCTGGC+CGG	0.503421	7.2:+31180559	MS.gene27495:CDS
AGATGGTGAGTGAGATCGGC+GGG	0.512562	7.2:-31180606	None:intergenic
GATATTTGAGTTCCTTCAAA+CGG	0.520675	7.2:-31180911	None:intergenic
TCCACTGGAGACGTCGCTTG+CGG	0.547190	7.2:+31180722	MS.gene27495:CDS
TTATGGGCTGAAGTGGTGTC+CGG	0.550025	7.2:-31180578	None:intergenic
TCCGCAAGCGACGTCTCCAG+TGG	0.551194	7.2:-31180723	None:intergenic
AGTGGTGTCCGGCCAGAGCG+TGG	0.557922	7.2:-31180567	None:intergenic
GACCGCGATGAAGAAGAAGC+TGG	0.558819	7.2:+31180752	MS.gene27495:CDS
TGACGCGAAGATGAAGGAGA+TGG	0.559497	7.2:+31180836	MS.gene27495:CDS
ACTGGAGACGTCGCTTGCGG+AGG	0.568299	7.2:+31180725	MS.gene27495:CDS
GGAGTGTTTATGGGCTGAAG+TGG	0.581810	7.2:-31180585	None:intergenic
GGAAAGATGGTGAGTGAGAT+CGG	0.586737	7.2:-31180610	None:intergenic
CAGTGGAGAAGTCACGGTGG+CGG	0.593899	7.2:-31180706	None:intergenic
TGAAATCACACCGATCCAGA+AGG	0.600226	7.2:+31180668	MS.gene27495:CDS
CTTATGCAAACACCCGCCAC+CGG	0.606622	7.2:+31180528	MS.gene27495:CDS
TTTCATACCTCTGATGTCGA+TGG	0.613539	7.2:-31181089	None:intergenic
CGTCTCCAGTGGAGAAGTCA+CGG	0.624834	7.2:-31180712	None:intergenic
GGAACTCAAATATCGATGCA+TGG	0.636808	7.2:+31180920	MS.gene27495:CDS
CTCCAGCTTCTTCTTCATCG+CGG	0.643638	7.2:-31180754	None:intergenic
AACAGCACCATCGACATCAG+AGG	0.666142	7.2:+31181082	MS.gene27495:CDS
AAGATGGTGAGTGAGATCGG+CGG	0.726270	7.2:-31180607	None:intergenic
CTCCAGTGGAGAAGTCACGG+TGG	0.790221	7.2:-31180709	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAACATAATTGATCGAGCT+TGG	-	chr7.2:31180649-31180668	None:intergenic	30.0%
	AAACATAATTGATCGAGCTT+GGG	-	chr7.2:31180648-31180667	None:intergenic	30.0%
	AGAAACAAAACGAAACTGAA+TGG	-	chr7.2:31180974-31180993	None:intergenic	30.0%
!	GATATTTGAGTTCCTTCAAA+CGG	-	chr7.2:31180914-31180933	None:intergenic	30.0%
!	TTCATGTTCCTTTTCTCTAA+GGG	-	chr7.2:31181048-31181067	None:intergenic	30.0%
!	TTTCATGTTCCTTTTCTCTA+AGG	-	chr7.2:31181049-31181068	None:intergenic	30.0%
	TATTCGAACCCTTAGAGAAA+AGG	+	chr7.2:31181037-31181056	MS.gene27495:CDS	35.0%
!!	TTTTCTCTAAGGGTTCGAAT+AGG	-	chr7.2:31181038-31181057	None:intergenic	35.0%
	AATTGATCGAGCTTGGGTTT+GGG	-	chr7.2:31180642-31180661	None:intergenic	40.0%
	CGATAGATTGTTCCGTTTGA+AGG	+	chr7.2:31180899-31180918	MS.gene27495:CDS	40.0%
	GGAACTCAAATATCGATGCA+TGG	+	chr7.2:31180920-31180939	MS.gene27495:CDS	40.0%
	TAATTGATCGAGCTTGGGTT+TGG	-	chr7.2:31180643-31180662	None:intergenic	40.0%
	GGAAAGATGGTGAGTGAGAT+CGG	-	chr7.2:31180613-31180632	None:intergenic	45.0%
	TGAAATCACACCGATCCAGA+AGG	+	chr7.2:31180668-31180687	MS.gene27495:CDS	45.0%
!	GGACAGTTTTCCTTCTGGAT+CGG	-	chr7.2:31180681-31180700	None:intergenic	45.0%
!	GTTTGGGCTTTGTGGAAAGA+TGG	-	chr7.2:31180626-31180645	None:intergenic	45.0%
!	TGATCGGACAGTTTTCCTTC+TGG	-	chr7.2:31180686-31180705	None:intergenic	45.0%
	AACAGCACCATCGACATCAG+AGG	+	chr7.2:31181082-31181101	MS.gene27495:CDS	50.0%
	AAGATGGTGAGTGAGATCGG+CGG	-	chr7.2:31180610-31180629	None:intergenic	50.0%
	CTCCAGCTTCTTCTTCATCG+CGG	-	chr7.2:31180757-31180776	None:intergenic	50.0%
	GTTGCTTGACGCGAAGATGA+AGG	+	chr7.2:31180830-31180849	MS.gene27495:CDS	50.0%
	TGACGCGAAGATGAAGGAGA+TGG	+	chr7.2:31180836-31180855	MS.gene27495:CDS	50.0%
!	GGAGTGTTTATGGGCTGAAG+TGG	-	chr7.2:31180588-31180607	None:intergenic	50.0%
!	TTATGGGCTGAAGTGGTGTC+CGG	-	chr7.2:31180581-31180600	None:intergenic	50.0%
!!	AGATCGGCGGGAGTGTTTAT+GGG	-	chr7.2:31180597-31180616	None:intergenic	50.0%
!!!	TGGCAGGATTTTCGGTGTTC+CGG	-	chr7.2:31180550-31180569	None:intergenic	50.0%
	AGATGGTGAGTGAGATCGGC+GGG	-	chr7.2:31180609-31180628	None:intergenic	55.0%
	CGTCTCCAGTGGAGAAGTCA+CGG	-	chr7.2:31180715-31180734	None:intergenic	55.0%
	CTTATGCAAACACCCGCCAC+CGG	+	chr7.2:31180528-31180547	MS.gene27495:CDS	55.0%
	GACCGCGATGAAGAAGAAGC+TGG	+	chr7.2:31180752-31180771	MS.gene27495:CDS	55.0%
	GAGCTTGGGTTTGGGCTTTG+TGG	-	chr7.2:31180634-31180653	None:intergenic	55.0%
!	CCAGAGCGTGGCAGGATTTT+CGG	-	chr7.2:31180558-31180577	None:intergenic	55.0%
!!	GAGATCGGCGGGAGTGTTTA+TGG	-	chr7.2:31180598-31180617	None:intergenic	55.0%
!!!	ATTTTCGGTGTTCCGGTGGC+GGG	-	chr7.2:31180543-31180562	None:intergenic	55.0%
!!!	CAGGATTTTCGGTGTTCCGG+TGG	-	chr7.2:31180547-31180566	None:intergenic	55.0%
!!!	GATTTTCGGTGTTCCGGTGG+CGG	-	chr7.2:31180544-31180563	None:intergenic	55.0%
	AAATCCTGCCACGCTCTGGC+CGG	+	chr7.2:31180559-31180578	MS.gene27495:CDS	60.0%
	CAGTGGAGAAGTCACGGTGG+CGG	-	chr7.2:31180709-31180728	None:intergenic	60.0%
	CCGAAAATCCTGCCACGCTC+TGG	+	chr7.2:31180555-31180574	MS.gene27495:CDS	60.0%
	CTCCAGTGGAGAAGTCACGG+TGG	-	chr7.2:31180712-31180731	None:intergenic	60.0%
	TCCACTGGAGACGTCGCTTG+CGG	+	chr7.2:31180722-31180741	MS.gene27495:CDS	60.0%
!	GAAGTCACGGTGGCGGTGAT+CGG	-	chr7.2:31180702-31180721	None:intergenic	60.0%
	ACTGGAGACGTCGCTTGCGG+AGG	+	chr7.2:31180725-31180744	MS.gene27495:CDS	65.0%
	CGCCACCGTGACTTCTCCAC+TGG	+	chr7.2:31180707-31180726	MS.gene27495:CDS	65.0%
	TCCGCAAGCGACGTCTCCAG+TGG	-	chr7.2:31180726-31180745	None:intergenic	65.0%
	AGTGGTGTCCGGCCAGAGCG+TGG	-	chr7.2:31180570-31180589	None:intergenic	70.0%
	GTGTCCGGCCAGAGCGTGGC+AGG	-	chr7.2:31180566-31180585	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	31180519	31181109	31180519	ID=MS.gene27495
chr7.2	mRNA	31180519	31181109	31180519	ID=MS.gene27495.t1;Parent=MS.gene27495
chr7.2	exon	31180519	31180941	31180519	ID=MS.gene27495.t1.exon1;Parent=MS.gene27495.t1
chr7.2	CDS	31180519	31180941	31180519	ID=cds.MS.gene27495.t1;Parent=MS.gene27495.t1
chr7.2	exon	31181026	31181109	31181026	ID=MS.gene27495.t1.exon2;Parent=MS.gene27495.t1
chr7.2	CDS	31181026	31181109	31181026	ID=cds.MS.gene27495.t1;Parent=MS.gene27495.t1

Gene Sequence

>MS.gene27495

ATGGCACAGCTTATGCAAACACCCGCCACCGGAACACCGAAAATCCTGCCACGCTCTGGCCGGACACCACTTCAGCCCATAAACACTCCCGCCGATCTCACTCACCATCTTTCCACAAAGCCCAAACCCAAGCTCGATCAATTATGTTTTGAAATCACACCGATCCAGAAGGAAAACTGTCCGATCACCGCCACCGTGACTTCTCCACTGGAGACGTCGCTTGCGGAGGAGCTGACCGCGATGAAGAAGAAGCTGGAGAGACTGAGAATAGACAAAGAAAAGACAGAGAAAATGCTGAAAGAGAGAGAAGCGTTGCTTGACGCGAAGATGAAGGAGATGGAAGAGAAAAGCGAGATTCAGAAGAATCTCGAAATTCAAGTCGATAGATTGTTCCGTTTGAAGGAACTCAAATATCGATGCATGGTGCGAACAATTAAGATTTCAAGTTTTCACCATTCAGTTTCGTTTTGTTTCTAATTTTGTTTTTTACTTTTTCGACTGTAACAGAGAGTTTCTCCTATTCGAACCCTTAGAGAAAAGGAACATGAAAAGATTATCAACGAAACAGCACCATCGACATCAGAGGTATGA

Protein sequence

>MS.gene27495.t1

MAQLMQTPATGTPKILPRSGRTPLQPINTPADLTHHLSTKPKPKLDQLCFEITPIQKENCPITATVTSPLETSLAEELTAMKKKLERLRIDKEKTEKMLKEREALLDAKMKEMEEKSEIQKNLEIQVDRLFRLKELKYRCMRVSPIRTLREKEHEKIINETAPSTSEV