Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27773.t1 | XP_013449291.1 | 98.3 | 363 | 6 | 0 | 13 | 375 | 70 | 432 | 3.60E-209 | 737.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27773.t1 | Q9Y4P3 | 29.5 | 325 | 207 | 7 | 20 | 344 | 79 | 381 | 1.1e-33 | 145.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27773.t1 | A0A072U288 | 98.3 | 363 | 6 | 0 | 13 | 375 | 70 | 432 | 2.6e-209 | 737.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene27773 | 0.853673 | 2.05E-61 | -1.69E-46 |
| MS.gene050317 | MS.gene27773 | 0.81018 | 1.31E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene27773 | 0.817573 | 3.08E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene27773 | 0.806792 | 6.93E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene27773 | 0.834177 | 3.48E-56 | -1.69E-46 |
| MS.gene057094 | MS.gene27773 | 0.808774 | 2.63E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene27773 | 0.800837 | 1.19E-48 | -1.69E-46 |
| MS.gene058639 | MS.gene27773 | 0.80969 | 1.67E-50 | -1.69E-46 |
| MS.gene05880 | MS.gene27773 | 0.819991 | 8.68E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene27773 | 0.802954 | 4.39E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene27773 | 0.812348 | 4.43E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene27773 | 0.822062 | 2.90E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene27773 | MS.gene22289 | PPI |
| MS.gene79299 | MS.gene27773 | PPI |
| MS.gene86578 | MS.gene27773 | PPI |
| MS.gene27773 | MS.gene21951 | PPI |
| MS.gene30824 | MS.gene27773 | PPI |
| MS.gene024701 | MS.gene27773 | PPI |
| MS.gene62770 | MS.gene27773 | PPI |
| MS.gene21679 | MS.gene27773 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27773.t1 | MTR_7g074890 | 98.347 | 363 | 6 | 0 | 13 | 375 | 70 | 432 | 0.0 | 738 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27773.t1 | AT4G00090 | 77.072 | 362 | 79 | 1 | 13 | 374 | 68 | 425 | 0.0 | 590 |
| MS.gene27773.t1 | AT4G32990 | 23.592 | 373 | 207 | 16 | 23 | 375 | 12 | 326 | 9.86e-12 | 65.9 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTCCTGATGGACGAAATT+TGG | 0.134819 | 7.3:-30553115 | MS.gene27773:CDS |
| TTCACCTGATGGAAAGATTT+TGG | 0.177641 | 7.3:-30546903 | MS.gene27773:CDS |
| GGGAACACCTTCAGTGTCTT+TGG | 0.241043 | 7.3:+30546980 | None:intergenic |
| TTGTGAAGATTGTGTGATTC+AGG | 0.278138 | 7.3:-30554303 | MS.gene27773:intron |
| GTGATTATTTGCTCAGAGTT+TGG | 0.280324 | 7.3:+30547458 | None:intergenic |
| CAGGAACGGACATCATTCTT+TGG | 0.298478 | 7.3:-30551028 | MS.gene27773:CDS |
| TGTCGAGACTGGAAAGGTTT+TGG | 0.301458 | 7.3:-30546846 | MS.gene27773:CDS |
| AAAGCAATAATTACTATGTT+TGG | 0.304101 | 7.3:-30551764 | MS.gene27773:CDS |
| AGGACGGTTCCTTAAGAATA+TGG | 0.309668 | 7.3:-30547427 | MS.gene27773:CDS |
| TTCAAATGTTATGCAGCTTA+AGG | 0.316013 | 7.3:-30549238 | MS.gene27773:CDS |
| TCACTTGCAGGTGACATTAC+AGG | 0.328172 | 7.3:-30546698 | MS.gene27773:intron |
| GGCCATGGTGATGCAGTTAC+AGG | 0.333345 | 7.3:-30553147 | MS.gene27773:CDS |
| ATTGATATTCCATATTCTTA+AGG | 0.334674 | 7.3:+30547418 | None:intergenic |
| GTAGAGTCACGAAGAGGAAT+TGG | 0.340083 | 7.3:+30546959 | None:intergenic |
| CACATGCCCCAAACTCTTCC+CGG | 0.345712 | 7.3:+30550995 | None:intergenic |
| TTTGGAGCGTCCGCTACTTA+TGG | 0.351553 | 7.3:-30551746 | MS.gene27773:CDS |
| GACTTATCTTCAGAAACTTT+AGG | 0.358893 | 7.3:+30551845 | None:intergenic |
| TTACCCATAGGAATAGCTTT+AGG | 0.374814 | 7.3:+30546662 | None:intergenic |
| TCAAATGTTATGCAGCTTAA+GGG | 0.416385 | 7.3:-30549237 | MS.gene27773:CDS |
| CGATGTTCCCACTTAATCTC+CGG | 0.421960 | 7.3:+30551803 | None:intergenic |
| CACAGAATTAATTTGCCTCC+TGG | 0.426988 | 7.3:-30551971 | MS.gene27773:CDS |
| TATTCTTAAGGAACCGTCCT+TGG | 0.428813 | 7.3:+30547430 | None:intergenic |
| TCTTGAAGTTCGCTATCATA+TGG | 0.443016 | 7.3:-30547011 | MS.gene27773:intron |
| GATGGACGAAATTTGGCAAC+AGG | 0.460035 | 7.3:-30553108 | MS.gene27773:intron |
| TATGCAGCTTAAGGGGCATA+AGG | 0.464062 | 7.3:-30549229 | MS.gene27773:intron |
| TGCATCACCATGGCCTTTCA+AGG | 0.464432 | 7.3:+30553155 | None:intergenic |
| GGTTGTTCATTGTACATGTA+TGG | 0.468235 | 7.3:-30551878 | MS.gene27773:CDS |
| ACAAGCAAAGCTTCCTCTCC+CGG | 0.468521 | 7.3:-30551822 | MS.gene27773:CDS |
| GTTGCCAAATTTCGTCCATC+AGG | 0.472098 | 7.3:+30553111 | None:intergenic |
| CGCCTCAGTATTTCACCTGA+TGG | 0.474853 | 7.3:-30546914 | MS.gene27773:CDS |
| GATTCTTGGGAATACCATCA+TGG | 0.476946 | 7.3:+30546807 | None:intergenic |
| GCTACTTATGGCAGTGCTGA+TGG | 0.488490 | 7.3:-30551734 | MS.gene27773:CDS |
| GCAGCCAAAATCTTTCCATC+AGG | 0.491333 | 7.3:+30546899 | None:intergenic |
| TAGAGTCACGAAGAGGAATT+GGG | 0.492229 | 7.3:+30546960 | None:intergenic |
| ATTCCTCTTCGTGACTCTAC+TGG | 0.494301 | 7.3:-30546956 | MS.gene27773:CDS |
| TTGGATTTGAATACCTTGAA+AGG | 0.495211 | 7.3:-30553168 | MS.gene27773:CDS |
| TTCCTCTCCCGGAGATTAAG+TGG | 0.497136 | 7.3:-30551811 | MS.gene27773:CDS |
| ATTGCAGTTGGAGGACCTCC+AGG | 0.502096 | 7.3:+30551953 | None:intergenic |
| GGGGCCTAAAGCTATTCCTA+TGG | 0.502990 | 7.3:-30546666 | MS.gene27773:CDS |
| CAGTGGTTAAGTGTCGAGAC+TGG | 0.507986 | 7.3:-30546857 | MS.gene27773:CDS |
| TTCAAGGTATTCAAATCCAA+TGG | 0.512823 | 7.3:+30553171 | None:intergenic |
| AGCGTTCACTGCAGACGTTA+AGG | 0.513771 | 7.3:-30550907 | MS.gene27773:intron |
| GTGACATTACAGGCATTGCA+TGG | 0.518069 | 7.3:-30546688 | MS.gene27773:CDS |
| GGTGAGAGTATTCAAGTTGG+AGG | 0.518079 | 7.3:-30552118 | MS.gene27773:CDS |
| TTGTTCAGAGTGCTGTGACT+TGG | 0.519779 | 7.3:-30547493 | MS.gene27773:intron |
| TCAGCAAATGCAATTGCAGT+TGG | 0.528703 | 7.3:+30551941 | None:intergenic |
| GATGTTCCCACTTAATCTCC+GGG | 0.529101 | 7.3:+30551804 | None:intergenic |
| TTGAATACCTTGAAAGGCCA+TGG | 0.533959 | 7.3:-30553162 | MS.gene27773:CDS |
| AGTCAAGCGGCATCATCCAT+TGG | 0.534347 | 7.3:-30553187 | MS.gene27773:CDS |
| CGGGAAGAGTTTGGGGCATG+TGG | 0.534451 | 7.3:-30550994 | MS.gene27773:CDS |
| GTGCAAACAGCTTGTGCTGA+TGG | 0.534976 | 7.3:-30552144 | MS.gene27773:intron |
| ATTCTTGGGAATACCATCAT+GGG | 0.534983 | 7.3:+30546808 | None:intergenic |
| ACGGACATCATTCTTTGGCA+TGG | 0.536612 | 7.3:-30551023 | MS.gene27773:CDS |
| GGGATATGTTTCTCTCCTGA+TGG | 0.536826 | 7.3:-30553126 | MS.gene27773:CDS |
| TTAAGAATATGGAATATCAA+TGG | 0.537043 | 7.3:-30547416 | MS.gene27773:intron |
| AGCTGCAATGAAACGTCCAT+TGG | 0.543756 | 7.3:+30550932 | None:intergenic |
| CAAATGTTATGCAGCTTAAG+GGG | 0.548411 | 7.3:-30549236 | MS.gene27773:CDS |
| TGACATTACAGGCATTGCAT+GGG | 0.551563 | 7.3:-30546687 | MS.gene27773:CDS |
| GCAAATGCAATTGCAGTTGG+AGG | 0.553636 | 7.3:+30551944 | None:intergenic |
| CTGGGAGATTGTATATTCAA+AGG | 0.557654 | 7.3:-30549280 | MS.gene27773:CDS |
| AGTGGTGAGAGTATTCAAGT+TGG | 0.558329 | 7.3:-30552121 | MS.gene27773:CDS |
| ACCACTGCAATGTGGAACCA+TGG | 0.567269 | 7.3:+30546873 | None:intergenic |
| TCCCACTTAATCTCCGGGAG+AGG | 0.568667 | 7.3:+30551809 | None:intergenic |
| GCTGCAATGAAACGTCCATT+GGG | 0.569996 | 7.3:+30550933 | None:intergenic |
| GAGATTGTATATTCAAAGGA+TGG | 0.573331 | 7.3:-30549276 | MS.gene27773:CDS |
| GCAAATAATCACTGCATCCA+AGG | 0.580008 | 7.3:-30547447 | MS.gene27773:CDS |
| TTAATTGCATCGTGTTCAGA+AGG | 0.582676 | 7.3:-30551704 | MS.gene27773:intron |
| GACATTACAGGCATTGCATG+GGG | 0.589619 | 7.3:-30546686 | MS.gene27773:CDS |
| TCCTCTCCCGGAGATTAAGT+GGG | 0.590082 | 7.3:-30551810 | MS.gene27773:CDS |
| TCTATATTGATGATTTGTCA+TGG | 0.596722 | 7.3:-30554329 | None:intergenic |
| GGGCCTAAAGCTATTCCTAT+GGG | 0.601225 | 7.3:-30546665 | MS.gene27773:intron |
| CCACTGCAATGTGGAACCAT+GGG | 0.607190 | 7.3:+30546874 | None:intergenic |
| GCCATGGTGATGCAGTTACA+GGG | 0.611622 | 7.3:-30553146 | MS.gene27773:CDS |
| GTTAAGTGTCGAGACTGGAA+AGG | 0.613018 | 7.3:-30546852 | MS.gene27773:CDS |
| GTGACAAGAAAGTGAAATTG+TGG | 0.614006 | 7.3:-30546269 | MS.gene27773:CDS |
| AGTTCGCTATCATATGGATG+AGG | 0.619477 | 7.3:-30547005 | MS.gene27773:CDS |
| GTGTCATCACACAATCTCAC+TGG | 0.620767 | 7.3:-30551899 | MS.gene27773:CDS |
| ATCAGCACTGCCATAAGTAG+CGG | 0.630073 | 7.3:+30551736 | None:intergenic |
| TGACAAGAAAGTGAAATTGT+GGG | 0.631764 | 7.3:-30546268 | MS.gene27773:CDS |
| CCCATGGTTCCACATTGCAG+TGG | 0.633715 | 7.3:-30546874 | MS.gene27773:CDS |
| ATGGCTACTATATCACCCAA+TGG | 0.633994 | 7.3:-30550948 | MS.gene27773:CDS |
| ATAATCACTGCATCCAAGGA+CGG | 0.635724 | 7.3:-30547443 | MS.gene27773:CDS |
| AACAGCTTGTGCTGATGGAG+TGG | 0.638047 | 7.3:-30552139 | MS.gene27773:intron |
| AGAATTAATTTGCCTCCTGG+AGG | 0.640059 | 7.3:-30551968 | MS.gene27773:CDS |
| TGAGGATCCAAAGACACTGA+AGG | 0.648025 | 7.3:-30546987 | MS.gene27773:CDS |
| GACACTTAACCACTGCAATG+TGG | 0.660431 | 7.3:+30546865 | None:intergenic |
| GTACCAGTAGAGTCACGAAG+AGG | 0.665126 | 7.3:+30546953 | None:intergenic |
| AACATAAGGATCAAGTCAAG+CGG | 0.669504 | 7.3:-30553200 | MS.gene27773:intron |
| TTCCATCAGGTGAAATACTG+AGG | 0.672850 | 7.3:+30546912 | None:intergenic |
| TCCCTGTAACTGCATCACCA+TGG | 0.687110 | 7.3:+30553145 | None:intergenic |
| ATTGTGTGATGACACAACAA+TGG | 0.716207 | 7.3:+30551907 | None:intergenic |
| ACATTACAGGCATTGCATGG+GGG | 0.726019 | 7.3:-30546685 | MS.gene27773:CDS |
| GCAGTTGGAGGACCTCCAGG+AGG | 0.740102 | 7.3:+30551956 | None:intergenic |
| GGTGCACTAGTAGTTCACGA+TGG | 0.754480 | 7.3:+30546240 | None:intergenic |
| GTGTGATGACACAACAATGG+AGG | 0.768302 | 7.3:+30551910 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 30546254 | 30554342 | 30546254 | ID=MS.gene27773 |
| chr7.3 | mRNA | 30546254 | 30554342 | 30546254 | ID=MS.gene27773.t1;Parent=MS.gene27773 |
| chr7.3 | exon | 30554304 | 30554342 | 30554304 | ID=MS.gene27773.t1.exon1;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30554304 | 30554342 | 30554304 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30553109 | 30553214 | 30553109 | ID=MS.gene27773.t1.exon2;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30553109 | 30553214 | 30553109 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30552096 | 30552156 | 30552096 | ID=MS.gene27773.t1.exon3;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30552096 | 30552156 | 30552096 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30551705 | 30551997 | 30551705 | ID=MS.gene27773.t1.exon4;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30551705 | 30551997 | 30551705 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30550908 | 30551047 | 30550908 | ID=MS.gene27773.t1.exon5;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30550908 | 30551047 | 30550908 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30549230 | 30549304 | 30549230 | ID=MS.gene27773.t1.exon6;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30549230 | 30549304 | 30549230 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30547417 | 30547507 | 30547417 | ID=MS.gene27773.t1.exon7;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30547417 | 30547507 | 30547417 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30546822 | 30547025 | 30546822 | ID=MS.gene27773.t1.exon8;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30546822 | 30547025 | 30546822 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30546666 | 30546710 | 30546666 | ID=MS.gene27773.t1.exon9;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30546666 | 30546710 | 30546666 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| chr7.3 | exon | 30546254 | 30546327 | 30546254 | ID=MS.gene27773.t1.exon10;Parent=MS.gene27773.t1 |
| chr7.3 | CDS | 30546254 | 30546327 | 30546254 | ID=cds.MS.gene27773.t1;Parent=MS.gene27773.t1 |
| Gene Sequence |
| Protein sequence |
