AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29117

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29117.t1 MTR_3g073590 96.209 765 21 1 1 765 46 802 0.0 1503
MS.gene29117.t1 MTR_5g061340 69.019 581 154 3 185 764 419 974 0.0 834
MS.gene29117.t1 MTR_1g035690 41.139 474 271 6 296 764 129 599 1.97e-121 377
MS.gene29117.t1 MTR_8g033290 41.314 472 272 5 295 764 154 622 3.42e-119 372
MS.gene29117.t1 MTR_8g078760 30.734 218 140 3 556 765 404 618 1.33e-22 103
MS.gene29117.t1 MTR_5g018820 36.000 150 89 1 553 695 398 547 1.28e-20 97.1
MS.gene29117.t1 MTR_8g079840 34.682 173 101 5 556 722 379 545 3.82e-19 92.4
MS.gene29117.t1 MTR_3g101340 32.184 174 104 3 556 722 321 487 1.39e-18 90.5
MS.gene29117.t1 MTR_3g101340 32.184 174 104 3 556 722 347 513 1.64e-18 90.1
MS.gene29117.t1 MTR_3g101340 30.220 182 105 3 556 722 346 520 2.91e-18 89.7
MS.gene29117.t1 MTR_5g066810 68.519 54 17 0 711 764 10 63 2.92e-18 79.7
MS.gene29117.t1 MTR_4g068920 34.211 152 93 2 552 696 407 558 1.10e-17 87.8
MS.gene29117.t1 MTR_5g017790 34.118 170 100 5 559 722 331 494 2.85e-17 86.3
MS.gene29117.t1 MTR_5g017790 30.693 202 127 6 528 722 287 482 2.99e-17 86.3
MS.gene29117.t1 MTR_1g021040 31.548 168 106 3 559 722 333 495 4.21e-17 85.9
MS.gene29117.t1 MTR_8g078760 36.800 125 72 1 556 673 404 528 2.59e-16 83.2
MS.gene29117.t1 MTR_5g012790 31.765 170 101 3 543 699 345 512 1.02e-15 81.6
MS.gene29117.t1 MTR_5g079910 30.729 192 125 4 552 740 289 475 1.11e-13 74.7
MS.gene29117.t1 MTR_4g058015 27.706 231 145 7 552 765 462 687 3.93e-13 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29117.t1 AT4G08550 62.522 571 192 4 196 764 140 690 0.0 720
MS.gene29117.t1 AT4G08550 55.692 571 177 6 196 764 140 636 0.0 607
MS.gene29117.t1 AT3G11920 39.413 477 280 5 295 764 155 629 6.98e-116 364
MS.gene29117.t1 AT5G66600 33.721 172 105 2 556 721 391 559 1.09e-23 107
MS.gene29117.t1 AT5G66600 33.721 172 105 2 556 721 391 559 1.09e-23 107
MS.gene29117.t1 AT5G66600 33.721 172 105 2 556 721 391 559 1.09e-23 107
MS.gene29117.t1 AT5G66600 33.721 172 105 2 556 721 391 559 1.09e-23 107
MS.gene29117.t1 AT5G66600 33.721 172 105 2 556 721 391 559 1.09e-23 107
MS.gene29117.t1 AT5G66600 33.721 172 105 2 556 721 371 539 1.09e-23 106
MS.gene29117.t1 AT5G66600 33.721 172 105 2 556 721 337 505 1.16e-23 106
MS.gene29117.t1 AT1G16750 30.081 246 134 7 545 765 293 525 6.44e-23 103
MS.gene29117.t1 AT3G13000 31.280 211 124 7 545 743 314 515 7.54e-22 100
MS.gene29117.t1 AT3G13000 31.280 211 124 7 545 743 343 544 9.45e-22 100
MS.gene29117.t1 AT3G13000 31.280 211 124 7 545 743 343 544 9.45e-22 100
MS.gene29117.t1 AT2G23700 30.594 219 140 5 556 765 484 699 6.58e-20 95.1
MS.gene29117.t1 AT5G66600 38.710 124 70 1 556 673 391 514 1.70e-18 90.1
MS.gene29117.t1 AT5G66600 38.710 124 70 1 556 673 391 514 1.70e-18 90.1
MS.gene29117.t1 AT5G66600 38.710 124 70 1 556 673 391 514 1.70e-18 90.1
MS.gene29117.t1 AT5G66600 38.710 124 70 1 556 673 391 514 1.70e-18 90.1
MS.gene29117.t1 AT4G37080 32.948 173 104 5 556 722 392 558 3.14e-18 89.7
MS.gene29117.t1 AT4G37080 32.948 173 104 5 556 722 392 558 3.14e-18 89.7
MS.gene29117.t1 AT4G37080 32.948 173 104 5 556 722 379 545 3.43e-18 89.4
MS.gene29117.t1 AT5G42690 32.558 172 106 4 555 722 321 486 1.20e-17 87.4
MS.gene29117.t1 AT5G42690 32.558 172 106 4 555 722 322 487 1.21e-17 87.4
MS.gene29117.t1 AT5G42690 32.558 172 106 4 555 722 322 487 1.21e-17 87.4
MS.gene29117.t1 AT5G42690 32.558 172 106 4 555 722 295 460 1.23e-17 87.4
MS.gene29117.t1 AT5G42690 32.558 172 106 4 555 722 270 435 1.32e-17 87.0
MS.gene29117.t1 AT5G47380 34.682 173 96 4 553 713 359 526 2.67e-17 86.7
MS.gene29117.t1 AT5G47380 33.898 177 100 4 553 717 359 530 2.85e-17 86.7
MS.gene29117.t1 AT5G47380 34.682 173 96 4 553 713 303 470 3.28e-17 86.3
MS.gene29117.t1 AT3G18900 31.098 164 105 3 540 695 214 377 5.11e-16 82.0
MS.gene29117.t1 AT3G18900 31.098 164 105 3 540 695 214 377 5.11e-16 82.0
MS.gene29117.t1 AT3G18900 31.098 164 105 3 540 695 295 458 6.54e-16 82.0
MS.gene29117.t1 AT3G18900 31.098 164 105 3 540 695 290 453 7.27e-16 81.6
MS.gene29117.t1 AT1G21060 26.957 230 153 6 545 765 266 489 6.10e-14 75.5
MS.gene29117.t1 AT1G21060 26.957 230 153 6 545 765 278 501 7.46e-14 75.5
MS.gene29117.t1 AT1G76620 33.553 152 86 4 556 696 299 446 2.18e-13 73.9
MS.gene29117.t1 AT2G39690 30.952 168 110 3 556 721 307 470 6.07e-13 72.4
MS.gene29117.t1 AT2G39690 30.952 168 110 3 556 721 281 444 7.76e-13 72.0
MS.gene29117.t1 AT2G39690 30.952 168 110 3 556 721 200 363 8.02e-13 71.6
MS.gene29117.t1 AT2G39690 30.357 168 110 3 556 721 307 469 1.40e-12 71.2
MS.gene29117.t1 AT2G39690 30.357 168 110 3 556 721 281 443 1.82e-12 70.9
MS.gene29117.t1 AT1G43020 32.667 150 87 5 556 695 218 363 2.65e-12 70.1
MS.gene29117.t1 AT3G18900 30.986 142 90 3 540 673 295 436 2.88e-12 70.1
MS.gene29117.t1 AT5G47380 35.338 133 71 3 553 673 359 488 4.53e-12 69.7
MS.gene29117.t1 AT5G47380 35.878 131 69 3 553 671 359 486 5.08e-12 69.7
MS.gene29117.t1 AT1G16750 37.374 99 59 1 545 640 293 391 5.53e-12 68.9

Find 172 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTTCTTAATATAATTTA+AGG 0.073218 3.4:-72217239 None:intergenic
TGCATCAAGGCTGAGATTTC+TGG 0.101988 3.4:+72218604 MS.gene29117:CDS
TGCGATAGGCTTTGGATTAA+TGG 0.164279 3.4:-72218576 None:intergenic
TTGGAAAGAAGGAAATTATT+TGG 0.198959 3.4:+72218974 MS.gene29117:CDS
TTCAATGTCACTGGAGATTC+TGG 0.202694 3.4:+72216680 MS.gene29117:CDS
TTTAGGTATAGCATAGATTT+TGG 0.208784 3.4:+72219754 MS.gene29117:intron
TCCAAGTGAGGACAACAATT+TGG 0.230260 3.4:+72216916 MS.gene29117:CDS
CATCCATGCAATCTTAATAT+TGG 0.235219 3.4:+72218934 MS.gene29117:CDS
CTTATCAGAAGATCAATATT+TGG 0.236747 3.4:+72217783 MS.gene29117:CDS
AATCCAATATTTCAACTCTT+TGG 0.237090 3.4:-72218859 None:intergenic
ACTATGCTGCGCCGCCTTTC+TGG 0.241084 3.4:+72217094 MS.gene29117:CDS
TCCCTTTCAGCTATTCAAAA+TGG 0.243224 3.4:+72219031 MS.gene29117:CDS
AACTGACTCAGCAATATTAT+TGG 0.243565 3.4:+72219825 MS.gene29117:CDS
GGAGAGTTCAAATATGTTAT+AGG 0.244778 3.4:+72218995 MS.gene29117:CDS
TTGGTATGTGGCACCCGATC+TGG 0.254719 3.4:+72219289 MS.gene29117:CDS
AATGGCTTCTTAAGGTTATA+TGG 0.266212 3.4:-72219079 None:intergenic
TGCAAAAGATAAACGCTTAA+AGG 0.270667 3.4:+72219105 MS.gene29117:CDS
GAGCACATCTACCCGTAAAT+TGG 0.283924 3.4:+72216985 MS.gene29117:CDS
CCTGATCTCCATCCAATTTA+CGG 0.285251 3.4:-72216997 None:intergenic
CTTCTTTCCAATGCTCCATC+TGG 0.285483 3.4:-72218962 None:intergenic
CAGTATAATCCTTCCTTTCA+TGG 0.286548 3.4:-72217332 None:intergenic
ATCCTTCCTTTCATGGCTAT+TGG 0.292169 3.4:-72217325 None:intergenic
AATTTGGACCCTGTATTTGA+TGG 0.308860 3.4:+72216932 MS.gene29117:CDS
GCTCCAAAGAGTTGAAATAT+TGG 0.318765 3.4:+72218856 MS.gene29117:CDS
ATATCATCCTCTCCAGAAAA+AGG 0.320824 3.4:-72217628 None:intergenic
GCAATTTCTGCGATAGGCTT+TGG 0.331252 3.4:-72218584 None:intergenic
TTCATCAACTGCCCCACTAA+TGG 0.342890 3.4:-72217653 None:intergenic
ATTTGATGGAACCGAGGTTC+CGG 0.359300 3.4:+72216946 MS.gene29117:CDS
GCTTGCCTCTTCTGAATTGA+AGG 0.361482 3.4:+72219852 MS.gene29117:CDS
ACGGAGTTGGAGAAGATATC+TGG 0.363743 3.4:+72217460 MS.gene29117:CDS
CTAGATGAAATGCACAGATA+TGG 0.373544 3.4:+72216815 MS.gene29117:CDS
ATCCATGCAATCTTAATATT+GGG 0.374358 3.4:+72218935 MS.gene29117:CDS
GACTTCTTAATATAATTTAA+GGG 0.376502 3.4:-72217238 None:intergenic
TAACATTGTGACATCACAAT+TGG 0.376676 3.4:-72218530 None:intergenic
TTTGATGGAACCGAGGTTCC+GGG 0.376989 3.4:+72216947 MS.gene29117:CDS
AGATTCTTGACACCCTAGTT+TGG 0.383221 3.4:-72217359 None:intergenic
TCCAAATTGTTGTCCTCACT+TGG 0.389670 3.4:-72216917 None:intergenic
TGCCATTCAGATTGAAGATC+AGG 0.390522 3.4:+72216526 MS.gene29117:CDS
ATGTCAGTAAGATTCTTAAA+TGG 0.394125 3.4:+72219425 MS.gene29117:CDS
TGGTATGTGGCACCCGATCT+GGG 0.395261 3.4:+72219290 MS.gene29117:CDS
AGAAGTTAATGAAGCCAAAC+TGG 0.400357 3.4:+72217180 MS.gene29117:CDS
GAGGATGATATTTCCATTAG+TGG 0.406602 3.4:+72217640 MS.gene29117:CDS
CAGATATGGACTGGATGATT+TGG 0.409841 3.4:+72216829 MS.gene29117:CDS
AGTGAAAATTTATTTGAAGA+TGG 0.410745 3.4:+72218476 MS.gene29117:intron
CCCTAAGTTTGACAGTAAAC+TGG 0.417217 3.4:+72216586 MS.gene29117:CDS
GTCAAGAATCTAGAATATTT+AGG 0.417224 3.4:+72217371 MS.gene29117:CDS
TCCAACTCCGTCTTTCTACC+AGG 0.418763 3.4:-72217448 None:intergenic
TAGTGGGGCAGTTGATGAAT+TGG 0.419111 3.4:+72217657 MS.gene29117:CDS
TTGCACCGAATGGCTTCTTA+AGG 0.419522 3.4:-72219087 None:intergenic
TGGCTTCTCAGAAACCAGTT+TGG 0.424844 3.4:-72217194 None:intergenic
AGAGTATTCTGGTGGTGATA+AGG 0.425577 3.4:+72216748 MS.gene29117:CDS
TTCATGGCTATTGGTGATGA+TGG 0.432688 3.4:-72217316 None:intergenic
TCCAGTTTACTGTCAAACTT+AGG 0.433560 3.4:-72216587 None:intergenic
ACCTCGGTTCCATCAAATAC+AGG 0.434676 3.4:-72216941 None:intergenic
ACCAAAGCAAAATGAATCAT+AGG 0.439045 3.4:-72219271 None:intergenic
CTGATCTCCATCCAATTTAC+GGG 0.439424 3.4:-72216996 None:intergenic
TCACGAGACCTCCACTTCTC+AGG 0.439974 3.4:-72219378 None:intergenic
TTCCAAGCAGATTTCTCCAC+TGG 0.442084 3.4:-72217214 None:intergenic
ATTATACTGTACACCAAACT+AGG 0.448111 3.4:+72217346 MS.gene29117:CDS
AATGTTGGTAAAGAGTATTC+TGG 0.448696 3.4:+72216737 MS.gene29117:CDS
GCCCTTCGATGCTATTCTCC+TGG 0.451401 3.4:+72219316 MS.gene29117:CDS
CCGTAAATTGGATGGAGATC+AGG 0.452393 3.4:+72216997 MS.gene29117:CDS
GCAAAATGAATCATAGGCTC+GGG 0.452440 3.4:-72219265 None:intergenic
CAGATGGAGCATTGGAAAGA+AGG 0.457598 3.4:+72218963 MS.gene29117:CDS
TTCCGGGGATGCAAGTTAAC+CGG 0.460048 3.4:+72216963 MS.gene29117:CDS
TTAATGGTAATAATACCCTT+TGG 0.464994 3.4:-72218560 None:intergenic
TTCAATGAAATATGTATTGG+AGG 0.465319 3.4:+72217508 MS.gene29117:CDS
TGATACATCGGACGTGATAA+CGG 0.466719 3.4:+72216610 MS.gene29117:CDS
AGACATGGAATCCACAGATA+TGG 0.468766 3.4:+72216874 MS.gene29117:CDS
TCGAAGGGCAGGCCCAGATC+GGG 0.471322 3.4:-72219302 None:intergenic
CTCCGGTTAACTTGCATCCC+CGG 0.471561 3.4:-72216965 None:intergenic
CAATATTAAGATTGCATGGA+TGG 0.471965 3.4:-72218933 None:intergenic
TTTAGCCTTAAAATTTATGT+TGG 0.474983 3.4:-72216777 None:intergenic
ATCGAAGGGCAGGCCCAGAT+CGG 0.479385 3.4:-72219303 None:intergenic
ATCCTGATCTTCAATCTGAA+TGG 0.479600 3.4:-72216528 None:intergenic
CTTGATGCAATTTCTGCGAT+AGG 0.479925 3.4:-72218590 None:intergenic
AGGTCTCGTGATTGATTTGA+CGG 0.480468 3.4:+72219390 MS.gene29117:CDS
AGGATGATATTTCCATTAGT+GGG 0.486028 3.4:+72217641 MS.gene29117:CDS
AGCAAAATGAATCATAGGCT+CGG 0.489457 3.4:-72219266 None:intergenic
TGAATCATAGGCTCGGGGTA+AGG 0.490288 3.4:-72219259 None:intergenic
TAGTTCAAACCCCAGTCATA+AGG 0.490858 3.4:-72219886 None:intergenic
TTGCAGCTTCTCAACCGCTT+GGG 0.498671 3.4:-72216492 None:intergenic
TTGCATGGAAGTGAGGAGTT+CGG 0.506332 3.4:+72218686 MS.gene29117:CDS
CATGGAAGATCAGCATTTGT+TGG 0.507056 3.4:+72216451 None:intergenic
AATAATATTGCTGAGTCAGT+TGG 0.507212 3.4:-72219823 None:intergenic
GAAGCCTATGCTATTGAAGA+TGG 0.508503 3.4:+72218641 MS.gene29117:CDS
CAGTCCAGGTGTGGTTGAGA+AGG 0.509358 3.4:+72217021 MS.gene29117:CDS
GAATCATAGGCTCGGGGTAA+GGG 0.509661 3.4:-72219258 None:intergenic
CTTGCAGCTTCTCAACCGCT+TGG 0.510135 3.4:-72216493 None:intergenic
GGAGAATAGCATCGAAGGGC+AGG 0.510556 3.4:-72219313 None:intergenic
CAGAGGGATTCAGTAACAAA+TGG 0.513968 3.4:+72217292 MS.gene29117:CDS
CAACAAATGCTGATCTTCCA+TGG 0.517186 3.4:-72216450 None:intergenic
ACTGCCTTCTCAACCACACC+TGG 0.521661 3.4:-72217025 None:intergenic
TTACATATCAGCCTTATGAC+TGG 0.523585 3.4:+72219875 MS.gene29117:CDS
CCAGTTTACTGTCAAACTTA+GGG 0.524030 3.4:-72216586 None:intergenic
TATTCAAAATGGAATATTGA+GGG 0.525670 3.4:+72219042 MS.gene29117:CDS
TAAGGTTATATGGAGGTCGT+TGG 0.529342 3.4:-72219069 None:intergenic
TGGAAAACAAAACTGATCAG+AGG 0.529835 3.4:+72217275 MS.gene29117:CDS
TCTCAGAATGTTGTTCATGA+TGG 0.530719 3.4:+72216563 MS.gene29117:CDS
TACATATCAGCCTTATGACT+GGG 0.530867 3.4:+72219876 MS.gene29117:CDS
CAGAAGATCAATATTTGGAA+AGG 0.531565 3.4:+72217788 MS.gene29117:CDS
TCAGCATTTGTTGGAGACAA+AGG 0.531880 3.4:+72216460 MS.gene29117:CDS
GACCCAATATTAAGATTGCA+TGG 0.533239 3.4:-72218937 None:intergenic
TCAATTCAGAAGAGGCAAGC+AGG 0.533585 3.4:-72219849 None:intergenic
AACTTGCATCCCCGGAACCT+CGG 0.534932 3.4:-72216957 None:intergenic
AGAAAGGCGGCGCAGCATAG+TGG 0.536077 3.4:-72217092 None:intergenic
CACCAATAGCCATGAAAGGA+AGG 0.542926 3.4:+72217323 MS.gene29117:CDS
CGCAATTTCCTGAGAAGTGG+AGG 0.544921 3.4:+72219370 MS.gene29117:CDS
GATTGAAGATCAGGATAAAG+AGG 0.545716 3.4:+72216535 MS.gene29117:CDS
ATTTCAGTTTCAGAATTGAT+CGG 0.549208 3.4:-72216710 None:intergenic
GGTGATGGGGTTTCAAATAA+CGG 0.551236 3.4:-72217604 None:intergenic
ATCCACAGATATGGATCAGA+AGG 0.552063 3.4:+72216883 MS.gene29117:CDS
AGCCAGTGGAGAAATCTGCT+TGG 0.553500 3.4:+72217212 MS.gene29117:CDS
TTCCAGGAGAATAGCATCGA+AGG 0.554025 3.4:-72219318 None:intergenic
GTGATAACGGATGACAATGA+TGG 0.554075 3.4:+72216623 MS.gene29117:CDS
CAATGTTACAATATTCCAAA+GGG 0.554541 3.4:+72218545 MS.gene29117:CDS
AAACAACTTGGGAACAGAAG+TGG 0.555017 3.4:-72217485 None:intergenic
GATGGAGATCAGGACAGTCC+AGG 0.555381 3.4:+72217007 MS.gene29117:CDS
GTATTCTGGTGGTGATAAGG+TGG 0.556223 3.4:+72216751 MS.gene29117:CDS
TTGAATAGCTGAAAGGGAGT+AGG 0.559780 3.4:-72219026 None:intergenic
GAAGTCATTTGATGTTGATG+TGG 0.561143 3.4:+72217255 MS.gene29117:CDS
TATAACCTTAAGAAGCCATT+CGG 0.563687 3.4:+72219082 MS.gene29117:CDS
CCCTGGTAGAAAGACGGAGT+TGG 0.563695 3.4:+72217447 MS.gene29117:CDS
TTAATATTGGGTCATCCAGA+TGG 0.565815 3.4:+72218947 MS.gene29117:CDS
ACAGATTGTACAAAATGCGC+AGG 0.567629 3.4:+72217722 MS.gene29117:CDS
TGCCTTCTGATCCATATCTG+TGG 0.567944 3.4:-72216885 None:intergenic
GAAACTGAAATTGATAATGT+TGG 0.569198 3.4:+72216722 MS.gene29117:CDS
ATTTACGGGTAGATGTGCTC+CGG 0.569546 3.4:-72216982 None:intergenic
TACAAGTTTGCATGGAAGTG+AGG 0.571856 3.4:+72218679 MS.gene29117:CDS
ATGGAAGTGAGGAGTTCGGC+AGG 0.573905 3.4:+72218690 MS.gene29117:CDS
AATAGCTGAAAGGGAGTAGG+TGG 0.575549 3.4:-72219023 None:intergenic
ACTCGCAATTTCCTGAGAAG+TGG 0.578546 3.4:+72219367 MS.gene29117:CDS
TTCTGGTGGTGATAAGGTGG+AGG 0.580331 3.4:+72216754 MS.gene29117:CDS
TCCAGGAGAATAGCATCGAA+GGG 0.585737 3.4:-72219317 None:intergenic
CTATTCAAAATGGAATATTG+AGG 0.586041 3.4:+72219041 MS.gene29117:CDS
ACAATGTTACAATATTCCAA+AGG 0.587793 3.4:+72218544 MS.gene29117:CDS
TGAAATGCACAGATATGGAC+TGG 0.589172 3.4:+72216820 MS.gene29117:CDS
TGTAACCTTCAATTCAGAAG+AGG 0.594840 3.4:-72219857 None:intergenic
GGAAAACAAAACTGATCAGA+GGG 0.600988 3.4:+72217276 MS.gene29117:CDS
GTTGATTATACAAGTTTGCA+TGG 0.601412 3.4:+72218671 MS.gene29117:CDS
TCAATATTTGGAAAGGCTAG+AGG 0.606043 3.4:+72217795 MS.gene29117:CDS
TCATCACCAATAGCCATGAA+AGG 0.614646 3.4:+72217319 MS.gene29117:CDS
CCAACTCCGTCTTTCTACCA+GGG 0.616219 3.4:-72217447 None:intergenic
ACATCTACCCGTAAATTGGA+TGG 0.616547 3.4:+72216989 MS.gene29117:CDS
ACATATCAGCCTTATGACTG+GGG 0.619604 3.4:+72219877 MS.gene29117:CDS
GAGTTCAAATATGTTATAGG+TGG 0.620548 3.4:+72218998 MS.gene29117:CDS
GGGTCATCCAGATGGAGCAT+TGG 0.621494 3.4:+72218955 MS.gene29117:CDS
TTGATGGAACCGAGGTTCCG+GGG 0.624334 3.4:+72216948 MS.gene29117:CDS
GGCAGATGAAATTCCAAGTG+AGG 0.627307 3.4:+72216904 MS.gene29117:CDS
CCCTGTATTTGATGGAACCG+AGG 0.628835 3.4:+72216940 MS.gene29117:CDS
TTATACTGTACACCAAACTA+GGG 0.629182 3.4:+72217347 MS.gene29117:CDS
GCATCCATCTTCAATAGCAT+AGG 0.630339 3.4:-72218645 None:intergenic
CTATCGCAGAAATTGCATCA+AGG 0.631626 3.4:+72218591 MS.gene29117:CDS
GGATGATATTTCCATTAGTG+GGG 0.635196 3.4:+72217642 MS.gene29117:CDS
CAGTAAACTGGATGATACAT+CGG 0.636432 3.4:+72216598 MS.gene29117:CDS
TGTATACCCTGGTAGAAAGA+CGG 0.637748 3.4:+72217441 MS.gene29117:CDS
ATTCAAAATGGAATATTGAG+GGG 0.652429 3.4:+72219043 MS.gene29117:CDS
CCTCGGTTCCATCAAATACA+GGG 0.653158 3.4:-72216940 None:intergenic
AGAGAACAACGGTAGAGACA+TGG 0.654893 3.4:+72216859 MS.gene29117:CDS
AGATCAGGACAGTCCAGGTG+TGG 0.655626 3.4:+72217012 MS.gene29117:CDS
GGCTTCTTAAGGTTATATGG+AGG 0.664268 3.4:-72219076 None:intergenic
ATCAACATAGATGTATACCC+TGG 0.667136 3.4:+72217430 MS.gene29117:CDS
TAACGGATGACAATGATGGA+AGG 0.676865 3.4:+72216627 MS.gene29117:CDS
CAAAATGAATCATAGGCTCG+GGG 0.678249 3.4:-72219264 None:intergenic
ACTGGTTTCTGAGAAGCCAG+TGG 0.679919 3.4:+72217198 MS.gene29117:CDS
TGTAAAGGAGAAAAGTGCAG+TGG 0.690641 3.4:+72217063 MS.gene29117:CDS
AAAAGTGATGAAGCTGCTGT+GGG 0.696516 3.4:+72217118 MS.gene29117:CDS
ATAGGCTTCGAACATTGCAG+AGG 0.705917 3.4:-72218627 None:intergenic
AAAGGAAGCAAGTTCCCAAG+CGG 0.717434 3.4:+72216478 MS.gene29117:CDS
TTGGCTAAAGCAGAGAACAA+CGG 0.720989 3.4:+72216848 MS.gene29117:CDS
GTTGGTAAAGAGTATTCTGG+TGG 0.735908 3.4:+72216740 MS.gene29117:CDS
GCAAACTTGTATAATCAACG+CGG 0.798022 3.4:-72218667 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAGTATAGAATTTTAAA+AGG - chr3.4:72219530-72219549 None:intergenic 10.0%
!! ATAATGTATTACATAAAATG+AGG + chr3.4:72218000-72218019 MS.gene29117:intron 15.0%
!! GACTTCTTAATATAATTTAA+GGG - chr3.4:72217241-72217260 None:intergenic 15.0%
!! TAAAAAAAGAAGAAAAACAA+TGG - chr3.4:72218290-72218309 None:intergenic 15.0%
!! TGACTTCTTAATATAATTTA+AGG - chr3.4:72217242-72217261 None:intergenic 15.0%
!!! ATATGATCTTTTAAATCTAT+GGG - chr3.4:72218144-72218163 None:intergenic 15.0%
!!! ATTAGTAAATATTGTTACTT+TGG - chr3.4:72218442-72218461 None:intergenic 15.0%
!!! TATATGATCTTTTAAATCTA+TGG - chr3.4:72218145-72218164 None:intergenic 15.0%
!!! TTTTTCAATGAAATATGTAT+TGG + chr3.4:72217505-72217524 MS.gene29117:CDS 15.0%
!! AAAAATTGACGAAGAATTAT+TGG + chr3.4:72219339-72219358 MS.gene29117:CDS 20.0%
!! AGTGAAAATTTATTTGAAGA+TGG + chr3.4:72218476-72218495 MS.gene29117:intron 20.0%
!! ATTTCATTGAAAAACAACTT+GGG - chr3.4:72217499-72217518 None:intergenic 20.0%
!! TATTCAAAATGGAATATTGA+GGG + chr3.4:72219042-72219061 MS.gene29117:CDS 20.0%
!! TATTTCATTGAAAAACAACT+TGG - chr3.4:72217500-72217519 None:intergenic 20.0%
!! TTAAATCTATGGGATATTTA+GGG - chr3.4:72218134-72218153 None:intergenic 20.0%
!! TTCTTTCAAATACAATTTGT+AGG + chr3.4:72218080-72218099 MS.gene29117:intron 20.0%
!! TTTAAATCTATGGGATATTT+AGG - chr3.4:72218135-72218154 None:intergenic 20.0%
!!! CTTCTTTTTTTAATTTTGTC+TGG + chr3.4:72218298-72218317 MS.gene29117:intron 20.0%
!!! TTTAGCCTTAAAATTTATGT+TGG - chr3.4:72216780-72216799 None:intergenic 20.0%
! AATCCAATATTTCAACTCTT+TGG - chr3.4:72218862-72218881 None:intergenic 25.0%
! ACAATGTTACAATATTCCAA+AGG + chr3.4:72218544-72218563 MS.gene29117:CDS 25.0%
! ATACCTAAATATGAACAATG+AGG - chr3.4:72219743-72219762 None:intergenic 25.0%
! ATCCATGCAATCTTAATATT+GGG + chr3.4:72218935-72218954 MS.gene29117:CDS 25.0%
! ATGTCAGTAAGATTCTTAAA+TGG + chr3.4:72219425-72219444 MS.gene29117:CDS 25.0%
! ATTCAAAATGGAATATTGAG+GGG + chr3.4:72219043-72219062 MS.gene29117:CDS 25.0%
! ATTCCTCATTGTTCATATTT+AGG + chr3.4:72219737-72219756 MS.gene29117:intron 25.0%
! ATTTCAGTTTCAGAATTGAT+CGG - chr3.4:72216713-72216732 None:intergenic 25.0%
! CAATGTTACAATATTCCAAA+GGG + chr3.4:72218545-72218564 MS.gene29117:CDS 25.0%
! CTATTCAAAATGGAATATTG+AGG + chr3.4:72219041-72219060 MS.gene29117:CDS 25.0%
! CTTATCAGAAGATCAATATT+TGG + chr3.4:72217783-72217802 MS.gene29117:CDS 25.0%
! GAAAAAAAATTCAATGTCAC+TGG + chr3.4:72216671-72216690 MS.gene29117:CDS 25.0%
! GTTAGAAATTGTGTTTACTT+TGG + chr3.4:72219691-72219710 MS.gene29117:intron 25.0%
! TGCTAGTGTATAAGATATTA+AGG - chr3.4:72219479-72219498 None:intergenic 25.0%
! TTAATGGTAATAATACCCTT+TGG - chr3.4:72218563-72218582 None:intergenic 25.0%
! TTCAATGAAATATGTATTGG+AGG + chr3.4:72217508-72217527 MS.gene29117:CDS 25.0%
! TTGGAAAGAAGGAAATTATT+TGG + chr3.4:72218974-72218993 MS.gene29117:CDS 25.0%
! TTTGATTACGTATGAATTTG+TGG - chr3.4:72217883-72217902 None:intergenic 25.0%
!! GTCAAGAATCTAGAATATTT+AGG + chr3.4:72217371-72217390 MS.gene29117:CDS 25.0%
!! TTTAGGTATAGCATAGATTT+TGG + chr3.4:72219754-72219773 MS.gene29117:intron 25.0%
!! TTTTACGTACAAAAAGACTT+AGG + chr3.4:72217398-72217417 MS.gene29117:CDS 25.0%
!!! CGTTGATTCATTTATTTGAT+AGG + chr3.4:72219228-72219247 MS.gene29117:intron 25.0%
!!! CTTTTTTTAATTTTGTCTGG+TGG + chr3.4:72218301-72218320 MS.gene29117:intron 25.0%
!!! GAAACTGAAATTGATAATGT+TGG + chr3.4:72216722-72216741 MS.gene29117:CDS 25.0%
!!! TTTCGAGCACAATTTATTTT+TGG + chr3.4:72218254-72218273 MS.gene29117:intron 25.0%
AATAATATTGCTGAGTCAGT+TGG - chr3.4:72219826-72219845 None:intergenic 30.0%
AATGGCTTCTTAAGGTTATA+TGG - chr3.4:72219082-72219101 None:intergenic 30.0%
ACCAAAGCAAAATGAATCAT+AGG - chr3.4:72219274-72219293 None:intergenic 30.0%
AGGATGATATTTCCATTAGT+GGG + chr3.4:72217641-72217660 MS.gene29117:CDS 30.0%
ATTATACTGTACACCAAACT+AGG + chr3.4:72217346-72217365 MS.gene29117:CDS 30.0%
CAATATTAAGATTGCATGGA+TGG - chr3.4:72218936-72218955 None:intergenic 30.0%
CAGAAGATCAATATTTGGAA+AGG + chr3.4:72217788-72217807 MS.gene29117:CDS 30.0%
CATCCATGCAATCTTAATAT+TGG + chr3.4:72218934-72218953 MS.gene29117:CDS 30.0%
CGCTCTTAAAAATCTTGTAA+AGG + chr3.4:72217048-72217067 MS.gene29117:CDS 30.0%
CTTAAAAACTTGGATGAATC+TGG + chr3.4:72217541-72217560 MS.gene29117:CDS 30.0%
GAACTTACAAAATGACTGAA+TGG - chr3.4:72218791-72218810 None:intergenic 30.0%
GAGTTCAAATATGTTATAGG+TGG + chr3.4:72218998-72219017 MS.gene29117:CDS 30.0%
GGAGAGTTCAAATATGTTAT+AGG + chr3.4:72218995-72219014 MS.gene29117:CDS 30.0%
GTTGATTATACAAGTTTGCA+TGG + chr3.4:72218671-72218690 MS.gene29117:CDS 30.0%
TAACATTGTGACATCACAAT+TGG - chr3.4:72218533-72218552 None:intergenic 30.0%
TACAAAAAGACTTAGGTTTG+TGG + chr3.4:72217405-72217424 MS.gene29117:CDS 30.0%
TATAACCTTAAGAAGCCATT+CGG + chr3.4:72219082-72219101 MS.gene29117:CDS 30.0%
TGCAAAAGATAAACGCTTAA+AGG + chr3.4:72219105-72219124 MS.gene29117:CDS 30.0%
TTATACTGTACACCAAACTA+GGG + chr3.4:72217347-72217366 MS.gene29117:CDS 30.0%
TTGTCTAATTGTCTTAGCTT+TGG + chr3.4:72217823-72217842 MS.gene29117:intron 30.0%
! AACTGACTCAGCAATATTAT+TGG + chr3.4:72219825-72219844 MS.gene29117:CDS 30.0%
! AATTCTTCGTCAATTTTTCC+AGG - chr3.4:72219337-72219356 None:intergenic 30.0%
! ATGCTATTTGTTGACATTGT+AGG + chr3.4:72218813-72218832 MS.gene29117:intron 30.0%
! ATTCATTTTGCTTTGGTATG+TGG + chr3.4:72219277-72219296 MS.gene29117:CDS 30.0%
! TCCATTTTGAATAGCTGAAA+GGG - chr3.4:72219035-72219054 None:intergenic 30.0%
! TGTCTAATTGTCTTAGCTTT+GGG + chr3.4:72217824-72217843 MS.gene29117:intron 30.0%
! TTCCATTTTGAATAGCTGAA+AGG - chr3.4:72219036-72219055 None:intergenic 30.0%
!! AATGTTGGTAAAGAGTATTC+TGG + chr3.4:72216737-72216756 MS.gene29117:CDS 30.0%
!! TCAGAGTTCTAAACTTCTAA+CGG - chr3.4:72218745-72218764 None:intergenic 30.0%
!!! AGGTTTACCAACTGTTTTTT+GGG + chr3.4:72217742-72217761 MS.gene29117:CDS 30.0%
!!! CTTTTGTTGTTGTTGTTGTT+TGG + chr3.4:72219135-72219154 MS.gene29117:intron 30.0%
AATTTGGACCCTGTATTTGA+TGG + chr3.4:72216932-72216951 MS.gene29117:CDS 35.0%
AGAAGTTAATGAAGCCAAAC+TGG + chr3.4:72217180-72217199 MS.gene29117:CDS 35.0%
AGCAAAATGAATCATAGGCT+CGG - chr3.4:72219269-72219288 None:intergenic 35.0%
ATATCATCCTCTCCAGAAAA+AGG - chr3.4:72217631-72217650 None:intergenic 35.0%
ATCAACATAGATGTATACCC+TGG + chr3.4:72217430-72217449 MS.gene29117:CDS 35.0%
ATCCTGATCTTCAATCTGAA+TGG - chr3.4:72216531-72216550 None:intergenic 35.0%
ATTGTGCTCGAAAGAATCTA+TGG - chr3.4:72218247-72218266 None:intergenic 35.0%
CAGTAAACTGGATGATACAT+CGG + chr3.4:72216598-72216617 MS.gene29117:CDS 35.0%
CAGTATAATCCTTCCTTTCA+TGG - chr3.4:72217335-72217354 None:intergenic 35.0%
CCAGTTTACTGTCAAACTTA+GGG - chr3.4:72216589-72216608 None:intergenic 35.0%
CTAGATGAAATGCACAGATA+TGG + chr3.4:72216815-72216834 MS.gene29117:CDS 35.0%
CTTGAGTGAGCTTAAAAACT+TGG + chr3.4:72217531-72217550 MS.gene29117:CDS 35.0%
GAAGTCATTTGATGTTGATG+TGG + chr3.4:72217255-72217274 MS.gene29117:CDS 35.0%
GACCCAATATTAAGATTGCA+TGG - chr3.4:72218940-72218959 None:intergenic 35.0%
GAGGATGATATTTCCATTAG+TGG + chr3.4:72217640-72217659 MS.gene29117:CDS 35.0%
GATTGAAGATCAGGATAAAG+AGG + chr3.4:72216535-72216554 MS.gene29117:CDS 35.0%
GCAAACTTGTATAATCAACG+CGG - chr3.4:72218670-72218689 None:intergenic 35.0%
GCTCCAAAGAGTTGAAATAT+TGG + chr3.4:72218856-72218875 MS.gene29117:CDS 35.0%
GGAAAACAAAACTGATCAGA+GGG + chr3.4:72217276-72217295 MS.gene29117:CDS 35.0%
GGATGATATTTCCATTAGTG+GGG + chr3.4:72217642-72217661 MS.gene29117:CDS 35.0%
TCAATATTTGGAAAGGCTAG+AGG + chr3.4:72217795-72217814 MS.gene29117:CDS 35.0%
TCCAGTTTACTGTCAAACTT+AGG - chr3.4:72216590-72216609 None:intergenic 35.0%
TCCCTTTCAGCTATTCAAAA+TGG + chr3.4:72219031-72219050 MS.gene29117:CDS 35.0%
TCTCAGAATGTTGTTCATGA+TGG + chr3.4:72216563-72216582 MS.gene29117:CDS 35.0%
TGGAAAACAAAACTGATCAG+AGG + chr3.4:72217275-72217294 MS.gene29117:CDS 35.0%
TGTAACCTTCAATTCAGAAG+AGG - chr3.4:72219860-72219879 None:intergenic 35.0%
TTAATATTGGGTCATCCAGA+TGG + chr3.4:72218947-72218966 MS.gene29117:CDS 35.0%
TTGTGCTCGAAAGAATCTAT+GGG - chr3.4:72218246-72218265 None:intergenic 35.0%
! GCCTATGATTCATTTTGCTT+TGG + chr3.4:72219270-72219289 MS.gene29117:CDS 35.0%
! GGAGGCCAACATAAATTTTA+AGG + chr3.4:72216772-72216791 MS.gene29117:CDS 35.0%
! TACATATCAGCCTTATGACT+GGG + chr3.4:72219876-72219895 MS.gene29117:CDS 35.0%
! TTAATTTTGTCTGGTGGTAC+AGG + chr3.4:72218307-72218326 MS.gene29117:intron 35.0%
! TTACATATCAGCCTTATGAC+TGG + chr3.4:72219875-72219894 MS.gene29117:CDS 35.0%
!! CAATCATGTGTATCGCTTTT+TGG + chr3.4:72218499-72218518 MS.gene29117:CDS 35.0%
!!! CAGGTTTACCAACTGTTTTT+TGG + chr3.4:72217741-72217760 MS.gene29117:CDS 35.0%
!!! CTTCATCACTTTTTCCAGAA+AGG - chr3.4:72217111-72217130 None:intergenic 35.0%
AAAAAGTGATGAAGCTGCTG+TGG + chr3.4:72217117-72217136 MS.gene29117:CDS 40.0%
AAAAGTGATGAAGCTGCTGT+GGG + chr3.4:72217118-72217137 MS.gene29117:CDS 40.0%
AAACAACTTGGGAACAGAAG+TGG - chr3.4:72217488-72217507 None:intergenic 40.0%
AATTTGTGGAATCGCTTGTG+TGG - chr3.4:72217869-72217888 None:intergenic 40.0%
ACAGATTGTACAAAATGCGC+AGG + chr3.4:72217722-72217741 MS.gene29117:CDS 40.0%
ACATCTACCCGTAAATTGGA+TGG + chr3.4:72216989-72217008 MS.gene29117:CDS 40.0%
AGACATGGAATCCACAGATA+TGG + chr3.4:72216874-72216893 MS.gene29117:CDS 40.0%
AGATTCTTGACACCCTAGTT+TGG - chr3.4:72217362-72217381 None:intergenic 40.0%
ATCCACAGATATGGATCAGA+AGG + chr3.4:72216883-72216902 MS.gene29117:CDS 40.0%
ATCCTTCCTTTCATGGCTAT+TGG - chr3.4:72217328-72217347 None:intergenic 40.0%
CAAAATGAATCATAGGCTCG+GGG - chr3.4:72219267-72219286 None:intergenic 40.0%
CAGAGGGATTCAGTAACAAA+TGG + chr3.4:72217292-72217311 MS.gene29117:CDS 40.0%
CCCTAAGTTTGACAGTAAAC+TGG + chr3.4:72216586-72216605 MS.gene29117:CDS 40.0%
CCTGATCTCCATCCAATTTA+CGG - chr3.4:72217000-72217019 None:intergenic 40.0%
CTATCGCAGAAATTGCATCA+AGG + chr3.4:72218591-72218610 MS.gene29117:CDS 40.0%
CTGATCTCCATCCAATTTAC+GGG - chr3.4:72216999-72217018 None:intergenic 40.0%
CTTGATGCAATTTCTGCGAT+AGG - chr3.4:72218593-72218612 None:intergenic 40.0%
GAAGCCTATGCTATTGAAGA+TGG + chr3.4:72218641-72218660 MS.gene29117:CDS 40.0%
GCAAAATGAATCATAGGCTC+GGG - chr3.4:72219268-72219287 None:intergenic 40.0%
GCATCCATCTTCAATAGCAT+AGG - chr3.4:72218648-72218667 None:intergenic 40.0%
GGCTTCTTAAGGTTATATGG+AGG - chr3.4:72219079-72219098 None:intergenic 40.0%
TAAGGTTATATGGAGGTCGT+TGG - chr3.4:72219072-72219091 None:intergenic 40.0%
TACAAGTTTGCATGGAAGTG+AGG + chr3.4:72218679-72218698 MS.gene29117:CDS 40.0%
TAGTTCAAACCCCAGTCATA+AGG - chr3.4:72219889-72219908 None:intergenic 40.0%
TCATCACCAATAGCCATGAA+AGG + chr3.4:72217319-72217338 MS.gene29117:CDS 40.0%
TCCAAATTGTTGTCCTCACT+TGG - chr3.4:72216920-72216939 None:intergenic 40.0%
TCCAAGTGAGGACAACAATT+TGG + chr3.4:72216916-72216935 MS.gene29117:CDS 40.0%
TGAAATGCACAGATATGGAC+TGG + chr3.4:72216820-72216839 MS.gene29117:CDS 40.0%
TGATACATCGGACGTGATAA+CGG + chr3.4:72216610-72216629 MS.gene29117:CDS 40.0%
TGCCATTCAGATTGAAGATC+AGG + chr3.4:72216526-72216545 MS.gene29117:CDS 40.0%
TGTATACCCTGGTAGAAAGA+CGG + chr3.4:72217441-72217460 MS.gene29117:CDS 40.0%
TTCAATGTCACTGGAGATTC+TGG + chr3.4:72216680-72216699 MS.gene29117:CDS 40.0%
TTGAATAGCTGAAAGGGAGT+AGG - chr3.4:72219029-72219048 None:intergenic 40.0%
TTGGCTAAAGCAGAGAACAA+CGG + chr3.4:72216848-72216867 MS.gene29117:CDS 40.0%
! ACATATCAGCCTTATGACTG+GGG + chr3.4:72219877-72219896 MS.gene29117:CDS 40.0%
! AGAGTATTCTGGTGGTGATA+AGG + chr3.4:72216748-72216767 MS.gene29117:CDS 40.0%
! CGTTTATCTTTTGCACCGAA+TGG - chr3.4:72219100-72219119 None:intergenic 40.0%
! TAACGGATGACAATGATGGA+AGG + chr3.4:72216627-72216646 MS.gene29117:CDS 40.0%
! TCAGCATTTGTTGGAGACAA+AGG + chr3.4:72216460-72216479 MS.gene29117:CDS 40.0%
! TGTAAAGGAGAAAAGTGCAG+TGG + chr3.4:72217063-72217082 MS.gene29117:CDS 40.0%
!! AGGTCTCGTGATTGATTTGA+CGG + chr3.4:72219390-72219409 MS.gene29117:CDS 40.0%
!! CAGATATGGACTGGATGATT+TGG + chr3.4:72216829-72216848 MS.gene29117:CDS 40.0%
!! GGTGATGGGGTTTCAAATAA+CGG - chr3.4:72217607-72217626 None:intergenic 40.0%
!! GTGATAACGGATGACAATGA+TGG + chr3.4:72216623-72216642 MS.gene29117:CDS 40.0%
!! GTTGGTAAAGAGTATTCTGG+TGG + chr3.4:72216740-72216759 MS.gene29117:CDS 40.0%
!! TGCGATAGGCTTTGGATTAA+TGG - chr3.4:72218579-72218598 None:intergenic 40.0%
!! TTCATGGCTATTGGTGATGA+TGG - chr3.4:72217319-72217338 None:intergenic 40.0%
!!! CATCACTTTTTCCAGAAAGG+CGG - chr3.4:72217108-72217127 None:intergenic 40.0%
!!! GTGGGTGATTTTGATGATGA+AGG + chr3.4:72217136-72217155 MS.gene29117:CDS 40.0%
AAAGGAAGCAAGTTCCCAAG+CGG + chr3.4:72216478-72216497 MS.gene29117:CDS 45.0%
AATAGCTGAAAGGGAGTAGG+TGG - chr3.4:72219026-72219045 None:intergenic 45.0%
ACCTCGGTTCCATCAAATAC+AGG - chr3.4:72216944-72216963 None:intergenic 45.0%
ACGGAGTTGGAGAAGATATC+TGG + chr3.4:72217460-72217479 MS.gene29117:CDS 45.0%
ACTCGCAATTTCCTGAGAAG+TGG + chr3.4:72219367-72219386 MS.gene29117:CDS 45.0%
AGAGAACAACGGTAGAGACA+TGG + chr3.4:72216859-72216878 MS.gene29117:CDS 45.0%
AGGTTGCTTACTCGTTGTGT+CGG - chr3.4:72219510-72219529 None:intergenic 45.0%
ATAGGCTTCGAACATTGCAG+AGG - chr3.4:72218630-72218649 None:intergenic 45.0%
ATTTACGGGTAGATGTGCTC+CGG - chr3.4:72216985-72217004 None:intergenic 45.0%
ATTTGATGGAACCGAGGTTC+CGG + chr3.4:72216946-72216965 MS.gene29117:CDS 45.0%
CACCAATAGCCATGAAAGGA+AGG + chr3.4:72217323-72217342 MS.gene29117:CDS 45.0%
CCGTAAATTGGATGGAGATC+AGG + chr3.4:72216997-72217016 MS.gene29117:CDS 45.0%
CCTCGGTTCCATCAAATACA+GGG - chr3.4:72216943-72216962 None:intergenic 45.0%
CTCCAGAAAAAGGTGGTGAT+GGG - chr3.4:72217621-72217640 None:intergenic 45.0%
CTTCTTTCCAATGCTCCATC+TGG - chr3.4:72218965-72218984 None:intergenic 45.0%
GAGCACATCTACCCGTAAAT+TGG + chr3.4:72216985-72217004 MS.gene29117:CDS 45.0%
GCAATTTCTGCGATAGGCTT+TGG - chr3.4:72218587-72218606 None:intergenic 45.0%
GCTTGCCTCTTCTGAATTGA+AGG + chr3.4:72219852-72219871 MS.gene29117:CDS 45.0%
GGCAGATGAAATTCCAAGTG+AGG + chr3.4:72216904-72216923 MS.gene29117:CDS 45.0%
GTCTGAGCCCAAAAAACAGT+TGG - chr3.4:72217752-72217771 None:intergenic 45.0%
TCAATTCAGAAGAGGCAAGC+AGG - chr3.4:72219852-72219871 None:intergenic 45.0%
TCATCCTCTCCAGAAAAAGG+TGG - chr3.4:72217628-72217647 None:intergenic 45.0%
TCTCCAGAAAAAGGTGGTGA+TGG - chr3.4:72217622-72217641 None:intergenic 45.0%
TGCATCAAGGCTGAGATTTC+TGG + chr3.4:72218604-72218623 MS.gene29117:CDS 45.0%
TGCCTTCTGATCCATATCTG+TGG - chr3.4:72216888-72216907 None:intergenic 45.0%
TGGCTTCTCAGAAACCAGTT+TGG - chr3.4:72217197-72217216 None:intergenic 45.0%
TTCATCAACTGCCCCACTAA+TGG - chr3.4:72217656-72217675 None:intergenic 45.0%
TTCCAAGCAGATTTCTCCAC+TGG - chr3.4:72217217-72217236 None:intergenic 45.0%
TTGCACCGAATGGCTTCTTA+AGG - chr3.4:72219090-72219109 None:intergenic 45.0%
TTGCATGGAAGTGAGGAGTT+CGG + chr3.4:72218686-72218705 MS.gene29117:CDS 45.0%
! GTATTCTGGTGGTGATAAGG+TGG + chr3.4:72216751-72216770 MS.gene29117:CDS 45.0%
! TCCAGAAAAAGGTGGTGATG+GGG - chr3.4:72217620-72217639 None:intergenic 45.0%
! TCCAGGAGAATAGCATCGAA+GGG - chr3.4:72219320-72219339 None:intergenic 45.0%
! TTCCAGGAGAATAGCATCGA+AGG - chr3.4:72219321-72219340 None:intergenic 45.0%
! TTTTGGCAAAAGCGAGCTTG+AGG + chr3.4:72219771-72219790 MS.gene29117:CDS 45.0%
!! ATCACCACCTTTTTCTGGAG+AGG + chr3.4:72217621-72217640 MS.gene29117:CDS 45.0%
!! CAGATGGAGCATTGGAAAGA+AGG + chr3.4:72218963-72218982 MS.gene29117:CDS 45.0%
!! TAGTGGGGCAGTTGATGAAT+TGG + chr3.4:72217657-72217676 MS.gene29117:CDS 45.0%
AGCCAGTGGAGAAATCTGCT+TGG + chr3.4:72217212-72217231 MS.gene29117:CDS 50.0%
CCAACTCCGTCTTTCTACCA+GGG - chr3.4:72217450-72217469 None:intergenic 50.0%
CCCTGTATTTGATGGAACCG+AGG + chr3.4:72216940-72216959 MS.gene29117:CDS 50.0%
CGCAATTTCCTGAGAAGTGG+AGG + chr3.4:72219370-72219389 MS.gene29117:CDS 50.0%
GAATCATAGGCTCGGGGTAA+GGG - chr3.4:72219261-72219280 None:intergenic 50.0%
TCCAACTCCGTCTTTCTACC+AGG - chr3.4:72217451-72217470 None:intergenic 50.0%
TGAATCATAGGCTCGGGGTA+AGG - chr3.4:72219262-72219281 None:intergenic 50.0%
TTCCGGGGATGCAAGTTAAC+CGG + chr3.4:72216963-72216982 MS.gene29117:CDS 50.0%
TTGCAGCTTCTCAACCGCTT+GGG - chr3.4:72216495-72216514 None:intergenic 50.0%
TTTGATGGAACCGAGGTTCC+GGG + chr3.4:72216947-72216966 MS.gene29117:CDS 50.0%
! ACCCCATCACCACCTTTTTC+TGG + chr3.4:72217616-72217635 MS.gene29117:CDS 50.0%
! ACTGGTTTCTGAGAAGCCAG+TGG + chr3.4:72217198-72217217 MS.gene29117:CDS 50.0%
! TTCTGGTGGTGATAAGGTGG+AGG + chr3.4:72216754-72216773 MS.gene29117:CDS 50.0%
AACTTGCATCCCCGGAACCT+CGG - chr3.4:72216960-72216979 None:intergenic 55.0%
ACTGCCTTCTCAACCACACC+TGG - chr3.4:72217028-72217047 None:intergenic 55.0%
AGATCAGGACAGTCCAGGTG+TGG + chr3.4:72217012-72217031 MS.gene29117:CDS 55.0%
ATGGAAGTGAGGAGTTCGGC+AGG + chr3.4:72218690-72218709 MS.gene29117:CDS 55.0%
CAGTCCAGGTGTGGTTGAGA+AGG + chr3.4:72217021-72217040 MS.gene29117:CDS 55.0%
CCCTGGTAGAAAGACGGAGT+TGG + chr3.4:72217447-72217466 MS.gene29117:CDS 55.0%
CTTGCAGCTTCTCAACCGCT+TGG - chr3.4:72216496-72216515 None:intergenic 55.0%
GATGGAGATCAGGACAGTCC+AGG + chr3.4:72217007-72217026 MS.gene29117:CDS 55.0%
GCCCTTCGATGCTATTCTCC+TGG + chr3.4:72219316-72219335 MS.gene29117:CDS 55.0%
GGGTCATCCAGATGGAGCAT+TGG + chr3.4:72218955-72218974 MS.gene29117:CDS 55.0%
TCACGAGACCTCCACTTCTC+AGG - chr3.4:72219381-72219400 None:intergenic 55.0%
TGGTATGTGGCACCCGATCT+GGG + chr3.4:72219290-72219309 MS.gene29117:CDS 55.0%
TTGATGGAACCGAGGTTCCG+GGG + chr3.4:72216948-72216967 MS.gene29117:CDS 55.0%
TTGGTATGTGGCACCCGATC+TGG + chr3.4:72219289-72219308 MS.gene29117:CDS 55.0%
! GGAGAATAGCATCGAAGGGC+AGG - chr3.4:72219316-72219335 None:intergenic 55.0%
!! CTCCGGTTAACTTGCATCCC+CGG - chr3.4:72216968-72216987 None:intergenic 55.0%
ACTATGCTGCGCCGCCTTTC+TGG + chr3.4:72217094-72217113 MS.gene29117:CDS 60.0%
AGAAAGGCGGCGCAGCATAG+TGG - chr3.4:72217095-72217114 None:intergenic 60.0%
ATCGAAGGGCAGGCCCAGAT+CGG - chr3.4:72219306-72219325 None:intergenic 60.0%
TCGAAGGGCAGGCCCAGATC+GGG - chr3.4:72219305-72219324 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 72216452 72219912 72216452 ID=MS.gene29117
chr3.4 mRNA 72216452 72219912 72216452 ID=MS.gene29117.t1;Parent=MS.gene29117
chr3.4 exon 72216452 72217816 72216452 ID=MS.gene29117.t1.exon1;Parent=MS.gene29117.t1
chr3.4 CDS 72216452 72217816 72216452 ID=cds.MS.gene29117.t1;Parent=MS.gene29117.t1
chr3.4 exon 72218329 72218384 72218329 ID=MS.gene29117.t1.exon2;Parent=MS.gene29117.t1
chr3.4 CDS 72218329 72218384 72218329 ID=cds.MS.gene29117.t1;Parent=MS.gene29117.t1
chr3.4 exon 72218478 72218711 72218478 ID=MS.gene29117.t1.exon3;Parent=MS.gene29117.t1
chr3.4 CDS 72218478 72218711 72218478 ID=cds.MS.gene29117.t1;Parent=MS.gene29117.t1
chr3.4 exon 72218835 72219126 72218835 ID=MS.gene29117.t1.exon4;Parent=MS.gene29117.t1
chr3.4 CDS 72218835 72219126 72218835 ID=cds.MS.gene29117.t1;Parent=MS.gene29117.t1
chr3.4 exon 72219250 72219446 72219250 ID=MS.gene29117.t1.exon5;Parent=MS.gene29117.t1
chr3.4 CDS 72219250 72219446 72219250 ID=cds.MS.gene29117.t1;Parent=MS.gene29117.t1
chr3.4 exon 72219759 72219912 72219759 ID=MS.gene29117.t1.exon6;Parent=MS.gene29117.t1
chr3.4 CDS 72219759 72219912 72219759 ID=cds.MS.gene29117.t1;Parent=MS.gene29117.t1
Gene Sequence

>MS.gene29117

ATGGAAGATCAGCATTTGTTGGAGACAAAGGAAGCAAGTTCCCAAGCGGTTGAGAAGCTGCAAGTAGATGCTTCTGCCATTCAGATTGAAGATCAGGATAAAGAGGCAAAATCTCAGAATGTTGTTCATGATGGCCCTAAGTTTGACAGTAAACTGGATGATACATCGGACGTGATAACGGATGACAATGATGGAAGGTCTAAGTCTGATACTGATGAAGAAAAAAAATTCAATGTCACTGGAGATTCTGGTTTAGCTCCGATCAATTCTGAAACTGAAATTGATAATGTTGGTAAAGAGTATTCTGGTGGTGATAAGGTGGAGGCCAACATAAATTTTAAGGCTAAAAGTGAAGAGACAAATCTAGATGAAATGCACAGATATGGACTGGATGATTTGGCTAAAGCAGAGAACAACGGTAGAGACATGGAATCCACAGATATGGATCAGAAGGCAGATGAAATTCCAAGTGAGGACAACAATTTGGACCCTGTATTTGATGGAACCGAGGTTCCGGGGATGCAAGTTAACCGGAGCACATCTACCCGTAAATTGGATGGAGATCAGGACAGTCCAGGTGTGGTTGAGAAGGCAGTCGCTCTTAAAAATCTTGTAAAGGAGAAAAGTGCAGTGGCAGTCTCCACTATGCTGCGCCGCCTTTCTGGAAAAAGTGATGAAGCTGCTGTGGGTGATTTTGATGATGAAGGCAATAATGTTTCAGATGTCTCAGAAGTTAATGAAGCCAAACTGGTTTCTGAGAAGCCAGTGGAGAAATCTGCTTGGAATCCCTTAAATTATATTAAGAAGTCATTTGATGTTGATGTGGAAAACAAAACTGATCAGAGGGATTCAGTAACAAATGGTCCATCATCACCAATAGCCATGAAAGGAAGGATTATACTGTACACCAAACTAGGGTGTCAAGAATCTAGAATATTTAGGCTATTTTTACGTACAAAAAGACTTAGGTTTGTGGAAATCAACATAGATGTATACCCTGGTAGAAAGACGGAGTTGGAGAAGATATCTGGATCCACTTCTGTTCCCAAGTTGTTTTTCAATGAAATATGTATTGGAGGCTTGAGTGAGCTTAAAAACTTGGATGAATCTGGAAAGCTTGATGAGAAGATTGACTTTCTTATTACTGAAGCACCGTTATTTGAAACCCCATCACCACCTTTTTCTGGAGAGGATGATATTTCCATTAGTGGGGCAGTTGATGAATTGGCTCTTGTTGTACACAAAATGAAAGAATCTATTACTGTGAAAGACAGATTGTACAAAATGCGCAGGTTTACCAACTGTTTTTTGGGCTCAGACGCTTTAGACTTCTTATCAGAAGATCAATATTTGGAAAGGCTAGAGGTAAGTTTGTCTAATTGTCTTAGCTTTGGGTCTAATGTGTCAATAAACACCACACAAGCGATTCCACAAATTCATACGTAATCAAACAAATCATACACTAAATATCACATATATTTACTTACATACAGTTTCATGATCAAACAAATCATACACTAAATTTCTCATAGATTCAGAAGATAATATATAATGTATTACATAAAATGAGGTCTAACTATACTTTGAATGACATAATCAAACAAATCATATCACACTTATCACATAGATTCTTTCAAATACAATTTGTAGGTCTTATCAAAACAATCAAAAAAATTATACCCTAAATATCCCATAGATTTAAAAGATCATATATAATAGTGTATTGCATAGAACTCTATTTTGTTATGATTTGAATAACACAATCAAACAAACAAATCATACACTCAATATCCCATAGATTCTTTCGAGCACAATTTATTTTTGGCTTCACAAAGCCATTGTTTTTCTTCTTTTTTTAATTTTGTCTGGTGGTACAGGCTGTTGAATTTGCTCGAAAGCTTGCTGACAAATTTTTCTTTCGACATGTTCTTGAGTGAGTCTATATACTTAGAGTTCTGATTTTTAATGTTTAATTTGTTTTGTTGTTCCAAAGTAACAATATTTACTAATTTTTGTTTTGATTCAGTGAAAATTTATTTGAAGATGGCAATCATGTGTATCGCTTTTTGGATGATGATCCAATTGTGATGTCACAATGTTACAATATTCCAAAGGGTATTATTACCATTAATCCAAAGCCTATCGCAGAAATTGCATCAAGGCTGAGATTTCTGGCCTCTGCAATGTTCGAAGCCTATGCTATTGAAGATGGATGCCGCGTTGATTATACAAGTTTGCATGGAAGTGAGGAGTTCGGCAGGTGTCTTCATTATTCTTTGTATTTTGTTTTCCGTTAGAAGTTTAGAACTCTGACGTTTTATACAATCTTTGATAGTCCATTCAGTCATTTTGTAAGTTCACATGCTATTTGTTGACATTGTAGGTATCTGAGAATAGTTGAAGAGCTCCAAAGAGTTGAAATATTGGATTTGTCTAGAGAAGAAAAGCTTGCATTCTTTATTAATCTTTATAACATGATGACCATCCATGCAATCTTAATATTGGGTCATCCAGATGGAGCATTGGAAAGAAGGAAATTATTTGGAGAGTTCAAATATGTTATAGGTGGTTCCACCTACTCCCTTTCAGCTATTCAAAATGGAATATTGAGGGGAAACCAACGACCTCCATATAACCTTAAGAAGCCATTCGGTGCAAAAGATAAACGCTTAAAGGTAAGCTACTTTTGTTGTTGTTGTTGTTTGGCACAATCACTTTCATGTTTAAATTAATTATTTTAGAATCACATGTAGACTTTCATCAGTTTTGATGCTCACGTTGATTCATTTATTTGATAGGTTGCACTCCCTTACCCCGAGCCTATGATTCATTTTGCTTTGGTATGTGGCACCCGATCTGGGCCTGCCCTTCGATGCTATTCTCCTGGAAAAATTGACGAAGAATTATTGGATGCAACTCGCAATTTCCTGAGAAGTGGAGGTCTCGTGATTGATTTGACGGCAAAGACTGCATATGTCAGTAAGATTCTTAAATGGTAAGTTCTTTTAAACAAAGAAAACAACTCCTTAATATCTTATACACTAGCACATTTTATCCGACACAACGAGTAAGCAACCTTTTAAAATTCTATACTTAATTGAATGTTTCAAATTTAAAGTACTGAAGATTTTAATGTGATATGTAAAATTTGTAGAAATGTCATTTAAAATTTCGTTTTGATATTTAATGTGATATGTAAAACTTAAACATGAACAATATTGTTGTTAAATGAAAGCAGTGTTAGAAATTGTGTTTACTTTGGTGATGTTGAAACTGAAAATAACTATTCCTCATTGTTCATATTTAGGTATAGCATAGATTTTGGCAAAAGCGAGCTTGAGGTGATCAAGCATGTGTCAAACTATTTAGATCCAACTGACTCAGCAATATTATTGGACCTGCTTGCCTCTTCTGAATTGAAGGTTACATATCAGCCTTATGACTGGGGTTTGAACTACTAG
Protein sequence

>MS.gene29117.t1

MEDQHLLETKEASSQAVEKLQVDASAIQIEDQDKEAKSQNVVHDGPKFDSKLDDTSDVITDDNDGRSKSDTDEEKKFNVTGDSGLAPINSETEIDNVGKEYSGGDKVEANINFKAKSEETNLDEMHRYGLDDLAKAENNGRDMESTDMDQKADEIPSEDNNLDPVFDGTEVPGMQVNRSTSTRKLDGDQDSPGVVEKAVALKNLVKEKSAVAVSTMLRRLSGKSDEAAVGDFDDEGNNVSDVSEVNEAKLVSEKPVEKSAWNPLNYIKKSFDVDVENKTDQRDSVTNGPSSPIAMKGRIILYTKLGCQESRIFRLFLRTKRLRFVEINIDVYPGRKTELEKISGSTSVPKLFFNEICIGGLSELKNLDESGKLDEKIDFLITEAPLFETPSPPFSGEDDISISGAVDELALVVHKMKESITVKDRLYKMRRFTNCFLGSDALDFLSEDQYLERLEAVEFARKLADKFFFRHVLDENLFEDGNHVYRFLDDDPIVMSQCYNIPKGIITINPKPIAEIASRLRFLASAMFEAYAIEDGCRVDYTSLHGSEEFGRYLRIVEELQRVEILDLSREEKLAFFINLYNMMTIHAILILGHPDGALERRKLFGEFKYVIGGSTYSLSAIQNGILRGNQRPPYNLKKPFGAKDKRLKVALPYPEPMIHFALVCGTRSGPALRCYSPGKIDEELLDATRNFLRSGGLVIDLTAKTAYVSKILKWYSIDFGKSELEVIKHVSNYLDPTDSAILLDLLASSELKVTYQPYDWGLNY