AlfalfaGEDB | MS.gene31579

M. sativa cultivar XinJiangDaYe / MS.gene31579

KEGG: K20715
GO: -
PFAM: PF13426.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31579.t1	AES67542.2	98	256	3	1	1	256	1	254	1.30E-136	495.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31579.t1	O48963	55.5	290	90	6	6	256	5	294	1.7e-73	277.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31579.t1	A0A072VCV1	98.0	256	3	1	1	256	1	254	9.1e-137	495.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene31579	0.857529	1.53E-62	-1.69E-46
MS.gene049369	MS.gene31579	0.839694	1.36E-57	-1.69E-46
MS.gene049907	MS.gene31579	0.803313	3.70E-49	-1.69E-46
MS.gene050122	MS.gene31579	0.826572	2.52E-54	-1.69E-46
MS.gene050128	MS.gene31579	0.826208	3.08E-54	-1.69E-46
MS.gene052188	MS.gene31579	0.834595	2.74E-56	-1.69E-46
MS.gene052340	MS.gene31579	0.807046	6.12E-50	-1.69E-46
MS.gene052485	MS.gene31579	0.808201	3.48E-50	-1.69E-46
MS.gene05316	MS.gene31579	0.822233	2.64E-53	-1.69E-46
MS.gene053813	MS.gene31579	0.847036	1.49E-59	-1.69E-46
MS.gene054123	MS.gene31579	0.805534	1.27E-49	-1.69E-46
MS.gene054349	MS.gene31579	0.801997	6.91E-49	-1.69E-46
MS.gene055116	MS.gene31579	0.812084	5.06E-51	-1.69E-46
MS.gene055802	MS.gene31579	0.807695	4.46E-50	-1.69E-46
MS.gene055909	MS.gene31579	0.810173	1.31E-50	-1.69E-46
MS.gene055911	MS.gene31579	0.813804	2.13E-51	-1.69E-46
MS.gene056422	MS.gene31579	0.813527	2.45E-51	-1.69E-46
MS.gene057396	MS.gene31579	0.803097	4.10E-49	-1.69E-46
MS.gene057448	MS.gene31579	0.83304	6.70E-56	-1.69E-46
MS.gene057449	MS.gene31579	0.831057	2.07E-55	-1.69E-46
MS.gene057450	MS.gene31579	0.826878	2.13E-54	-1.69E-46
MS.gene057537	MS.gene31579	0.846397	2.23E-59	-1.69E-46
MS.gene057749	MS.gene31579	0.825287	5.09E-54	-1.69E-46
MS.gene057823	MS.gene31579	0.827666	1.38E-54	-1.69E-46
MS.gene057927	MS.gene31579	0.814794	1.29E-51	-1.69E-46
MS.gene058075	MS.gene31579	0.833218	6.05E-56	-1.69E-46
MS.gene058459	MS.gene31579	0.827815	1.27E-54	-1.69E-46
MS.gene05858	MS.gene31579	0.823997	1.02E-53	-1.69E-46
MS.gene058928	MS.gene31579	0.815526	8.84E-52	-1.69E-46
MS.gene058949	MS.gene31579	0.862425	5.12E-64	-1.69E-46
MS.gene059366	MS.gene31579	0.818134	2.30E-52	-1.69E-46
MS.gene059674	MS.gene31579	0.850029	2.21E-60	-1.69E-46
MS.gene059688	MS.gene31579	0.817714	2.86E-52	-1.69E-46
MS.gene059692	MS.gene31579	0.810737	9.93E-51	-1.69E-46
MS.gene059695	MS.gene31579	0.815802	7.67E-52	-1.69E-46
MS.gene059711	MS.gene31579	0.830907	2.25E-55	-1.69E-46
MS.gene059807	MS.gene31579	0.861881	7.51E-64	-1.69E-46
MS.gene059876	MS.gene31579	0.80624	9.06E-50	-1.69E-46
MS.gene06037	MS.gene31579	0.800927	1.15E-48	-1.69E-46
MS.gene060495	MS.gene31579	0.849591	2.93E-60	-1.69E-46
MS.gene060837	MS.gene31579	0.81221	4.75E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31579.t1	MTR_2g095980	98.047	256	3	1	1	256	1	254	1.25e-179	519
MS.gene31579.t1	MTR_2g095980	42.593	108	62	0	149	256	430	537	2.89e-26	107
MS.gene31579.t1	MTR_2g095980	98.047	256	3	1	1	256	1	254	6.61e-179	518
MS.gene31579.t1	MTR_2g095980	42.593	108	62	0	149	256	430	537	3.20e-26	107
MS.gene31579.t1	MTR_4g061610	66.667	258	61	7	17	256	11	261	1.10e-102	320
MS.gene31579.t1	MTR_4g061610	42.593	108	62	0	149	256	448	555	1.51e-26	108
MS.gene31579.t1	MTR_8g070530	59.669	181	66	3	79	256	48	224	1.23e-62	211
MS.gene31579.t1	MTR_8g070530	41.667	108	63	0	149	256	401	508	2.14e-25	105
MS.gene31579.t1	MTR_2g095980	42.593	108	62	0	149	256	175	282	2.32e-26	107
MS.gene31579.t1	MTR_5g038420	47.000	100	53	0	157	256	249	348	2.61e-26	105
MS.gene31579.t1	MTR_5g038420	42.353	85	49	0	161	245	29	113	5.58e-18	82.8
MS.gene31579.t1	MTR_5g038420	48.958	96	49	0	158	253	250	345	4.23e-26	105
MS.gene31579.t1	MTR_5g038420	42.353	85	49	0	161	245	29	113	1.33e-17	82.0
MS.gene31579.t1	MTR_5g038420	48.936	94	48	0	157	250	249	342	7.14e-26	104
MS.gene31579.t1	MTR_5g038420	42.353	85	49	0	161	245	29	113	4.08e-18	82.8
MS.gene31579.t1	MTR_8g105590	47.170	106	47	1	160	256	63	168	1.37e-23	99.8
MS.gene31579.t1	MTR_2g036510	40.000	105	54	1	161	256	42	146	4.11e-20	89.7
MS.gene31579.t1	MTR_5g038420	42.353	85	49	0	161	245	29	113	2.58e-18	82.8
MS.gene31579.t1	MTR_5g038420	53.061	49	23	0	157	205	249	297	2.70e-11	62.8
MS.gene31579.t1	MTR_5g038420	42.353	85	49	0	161	245	29	113	2.83e-18	82.8
MS.gene31579.t1	MTR_5g038420	46.875	64	34	0	157	220	249	312	2.96e-13	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31579.t1	AT3G45780	55.556	288	89	5	8	256	7	294	6.75e-93	294
MS.gene31579.t1	AT3G45780	40.741	108	64	0	149	256	465	572	2.11e-24	102
MS.gene31579.t1	AT3G45780	55.556	288	89	5	8	256	7	294	6.75e-93	294
MS.gene31579.t1	AT3G45780	40.741	108	64	0	149	256	465	572	2.11e-24	102
MS.gene31579.t1	AT5G58140	47.082	257	108	6	1	256	1	230	9.38e-62	206
MS.gene31579.t1	AT5G58140	33.333	180	118	1	79	256	307	486	5.87e-26	106
MS.gene31579.t1	AT5G58140	47.082	257	108	6	1	256	1	230	1.26e-60	205
MS.gene31579.t1	AT5G58140	33.333	180	118	1	79	256	307	486	1.10e-25	106
MS.gene31579.t1	AT5G58140	47.082	257	108	6	1	256	1	230	1.92e-60	205
MS.gene31579.t1	AT5G58140	33.333	180	118	1	79	256	307	486	1.15e-25	105
MS.gene31579.t1	AT5G58140	47.082	257	108	6	1	256	1	230	1.92e-60	205
MS.gene31579.t1	AT5G58140	33.333	180	118	1	79	256	307	486	1.15e-25	105
MS.gene31579.t1	AT5G58140	47.082	257	108	6	1	256	1	230	1.92e-60	205
MS.gene31579.t1	AT5G58140	33.333	180	118	1	79	256	307	486	1.15e-25	105
MS.gene31579.t1	AT5G58140	47.082	257	108	6	1	256	1	230	1.92e-60	205
MS.gene31579.t1	AT5G58140	33.333	180	118	1	79	256	307	486	1.15e-25	105
MS.gene31579.t1	AT5G58140	47.082	257	108	6	1	256	1	230	1.92e-60	205
MS.gene31579.t1	AT5G58140	33.333	180	118	1	79	256	307	486	1.15e-25	105
MS.gene31579.t1	AT2G02710	42.727	110	63	0	141	250	241	350	3.74e-27	108
MS.gene31579.t1	AT2G02710	41.667	108	62	1	150	256	28	135	3.20e-22	94.7
MS.gene31579.t1	AT2G02710	42.105	114	64	1	141	252	241	354	4.91e-26	104
MS.gene31579.t1	AT2G02710	41.667	108	62	1	150	256	28	135	2.13e-22	94.7
MS.gene31579.t1	AT2G02710	41.964	112	63	1	141	250	241	352	2.19e-25	103
MS.gene31579.t1	AT2G02710	41.667	108	62	1	150	256	28	135	2.95e-22	95.1
MS.gene31579.t1	AT1G68050	46.226	106	48	1	160	256	55	160	3.66e-25	104
MS.gene31579.t1	AT2G02710	41.667	108	62	1	150	256	28	135	1.58e-22	94.7
MS.gene31579.t1	AT2G02710	40.789	76	45	0	141	216	241	316	7.31e-14	70.5
MS.gene31579.t1	AT2G18915	46.939	98	43	1	161	249	47	144	5.33e-21	92.4
MS.gene31579.t1	AT5G57360	33.548	155	72	3	111	256	19	151	4.13e-19	86.7
MS.gene31579.t1	AT5G57360	33.548	155	72	3	111	256	19	151	5.00e-19	86.7
MS.gene31579.t1	AT2G18915	43.820	89	49	1	161	249	47	134	2.16e-15	75.9

Find 82 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAACTCTGGCAGAGTTTC+CGG	0.217366	2.1:-7686542	MS.gene31579:CDS
GGCAATGTGCTCAAGTTAAT+TGG	0.247139	2.1:-7685836	MS.gene31579:CDS
CTTATCTTTGCCACGTCTTC+AGG	0.250056	2.1:+7685968	None:intergenic
TCCCACTCCTTGTGGTTTCC+CGG	0.270958	2.1:+7686622	None:intergenic
TCAACAAACTTTCGTGGTTT+CGG	0.309777	2.1:-7686444	MS.gene31579:CDS
AGAAGGATGGAACTCCATTC+TGG	0.318494	2.1:-7685895	MS.gene31579:CDS
TGCTCTCTGTGCCGCGGCGC+CGG	0.321129	2.1:+7686682	None:intergenic
TGCATACATAATCGGGTAAT+CGG	0.321776	2.1:+7686409	None:intergenic
CCTGCACTTGCATACATAAT+CGG	0.326867	2.1:+7686401	None:intergenic
CTCAGAACAGACGCTGAAAC+CGG	0.376841	2.1:-7686641	MS.gene31579:CDS
ATGAAGTCACCAACACTTCA+TGG	0.386276	2.1:-7686727	MS.gene31579:CDS
TTCAAATCCTCCGAAACTCT+TGG	0.389814	2.1:+7686485	None:intergenic
ACGCGGGTTTCCAAGAGTTT+CGG	0.390873	2.1:-7686495	MS.gene31579:CDS
GAGAGCAGCCGAGTGGGGTT+TGG	0.401355	2.1:-7686666	MS.gene31579:CDS
CACTTCATGGATGGCAATCA+AGG	0.403055	2.1:-7686714	MS.gene31579:CDS
AAGAAGTCATAGGAAGAAAC+TGG	0.423723	2.1:-7686349	MS.gene31579:intron
CATAATCGGGTAATCGGGTT+TGG	0.439804	2.1:+7686415	None:intergenic
CTGAAGACGTGGCAAAGATA+AGG	0.443688	2.1:-7685967	MS.gene31579:CDS
TCCATTCTGTTCATCGTCAC+CGG	0.456704	2.1:+7686589	None:intergenic
ATTGCCATCATCATCCTTGA+TGG	0.459706	2.1:+7685853	None:intergenic
GGTGAAGTTGAGTTGCTTGT+TGG	0.459968	2.1:+7686827	None:intergenic
GCAATGTGCTCAAGTTAATT+GGG	0.462004	2.1:-7685835	None:intergenic
TATACTTCAAAAGAAGTCAT+AGG	0.464110	2.1:-7686359	MS.gene31579:CDS
CCGATTATGTATGCAAGTGC+AGG	0.470820	2.1:-7686401	MS.gene31579:CDS
GCATACATAATCGGGTAATC+GGG	0.476217	2.1:+7686410	None:intergenic
TGAAGACGTGGCAAAGATAA+GGG	0.478455	2.1:-7685966	MS.gene31579:CDS
CACACTCACACCTGAAAACA+TGG	0.483295	2.1:-7686790	MS.gene31579:CDS
CTGCACTTGCATACATAATC+GGG	0.483989	2.1:+7686402	None:intergenic
CGAGGATCCCTCGGAAATGA+TGG	0.487926	2.1:+7686872	None:intergenic
TCTCACTATTTCACCCATCA+AGG	0.490552	2.1:-7685867	MS.gene31579:CDS
GCAGGTTTCTTCAACATGAC+GGG	0.495681	2.1:-7686383	MS.gene31579:CDS
GCAATCAAGGAAGGAGAAAC+CGG	0.495765	2.1:-7686701	MS.gene31579:CDS
ATGGAAAGTTCTCCGGCAAA+AGG	0.497594	2.1:-7686571	MS.gene31579:CDS
TAAGGGAAGCATTAGAAGGT+GGG	0.503095	2.1:-7685949	MS.gene31579:CDS
AAGATAAGGGAAGCATTAGA+AGG	0.506363	2.1:-7685953	MS.gene31579:CDS
AAAAGGAATTCTAATAACTC+TGG	0.516172	2.1:-7686554	MS.gene31579:CDS
TTTCTAACTCCCACTCCTTG+TGG	0.526938	2.1:+7686614	None:intergenic
CCTGAAAACATGGACAGAAA+CGG	0.538440	2.1:-7686780	MS.gene31579:CDS
GAACAGAATGGAAAGTTCTC+CGG	0.541170	2.1:-7686578	MS.gene31579:CDS
TTGTTGGATTGAATACTTCG+AGG	0.544699	2.1:+7686843	None:intergenic
GGCACAGAGAGCAGCCGAGT+GGG	0.549650	2.1:-7686672	MS.gene31579:CDS
GAAGGTGGGAAAAGTTATTG+TGG	0.550813	2.1:-7685935	MS.gene31579:CDS
CGGGTTTCCAAGAGTTTCGG+AGG	0.554496	2.1:-7686492	MS.gene31579:CDS
TCACCCATCAAGGATGATGA+TGG	0.556770	2.1:-7685857	MS.gene31579:CDS
TGCAGGTTTCTTCAACATGA+CGG	0.557914	2.1:-7686384	MS.gene31579:CDS
GTGGGAAAAGTTATTGTGGA+AGG	0.562063	2.1:-7685931	MS.gene31579:CDS
TGTTGGATTGAATACTTCGA+GGG	0.564410	2.1:+7686844	None:intergenic
TTACTGAATTACAAGAAGGA+TGG	0.565513	2.1:-7685908	MS.gene31579:CDS
ATAAGGGAAGCATTAGAAGG+TGG	0.565911	2.1:-7685950	MS.gene31579:CDS
CTGACGGCGGTGATCCACGC+GGG	0.572568	2.1:-7686511	MS.gene31579:CDS
AGGGATCCTCGAGGATCCCT+CGG	0.573765	2.1:+7686863	None:intergenic
TCAGAACAGACGCTGAAACC+GGG	0.578722	2.1:-7686640	MS.gene31579:CDS
TCCGGTGACGATGAACAGAA+TGG	0.582218	2.1:-7686590	MS.gene31579:CDS
AGTGTTGGTGACTTCATCGC+TGG	0.594943	2.1:+7686733	None:intergenic
AACAAGCAACTCAACTTCAC+CGG	0.596639	2.1:-7686825	MS.gene31579:CDS
CTCGGCTGCTCTCTGTGCCG+CGG	0.596930	2.1:+7686676	None:intergenic
TTGCCATCATCATCCTTGAT+GGG	0.598307	2.1:+7685854	None:intergenic
TCATGGATGGCAATCAAGGA+AGG	0.600999	2.1:-7686710	MS.gene31579:CDS
ACCGCCGTCAGAAGAGTCAC+CGG	0.604229	2.1:+7686523	None:intergenic
GATTGCCATCCATGAAGTGT+TGG	0.607024	2.1:+7686718	None:intergenic
AAGGTTACTGAATTACAAGA+AGG	0.607960	2.1:-7685912	MS.gene31579:CDS
CACAAAGACCATCATTTCCG+AGG	0.610511	2.1:-7686880	MS.gene31579:CDS
AACCGGGAAACCACAAGGAG+TGG	0.616718	2.1:-7686624	MS.gene31579:CDS
GGAGTGGGAGTTAGAAACTC+CGG	0.618718	2.1:-7686608	MS.gene31579:CDS
GGAAGGAGAAACCGGCGCCG+CGG	0.624207	2.1:-7686693	MS.gene31579:CDS
AGTCACCAACACTTCATGGA+TGG	0.626274	2.1:-7686723	MS.gene31579:CDS
ACCGGGAAACCACAAGGAGT+GGG	0.634059	2.1:-7686623	MS.gene31579:CDS
ATAGTGAGAAGATTCCAGAA+TGG	0.635201	2.1:+7685881	None:intergenic
TCTGAGCACCAAACCCCACT+CGG	0.636849	2.1:+7686658	None:intergenic
ACAAAGACCATCATTTCCGA+GGG	0.642926	2.1:-7686879	MS.gene31579:CDS
TCCGGTGACTCTTCTGACGG+CGG	0.644875	2.1:-7686524	MS.gene31579:CDS
GGTCTTTGTGATGAAGATGA+TGG	0.645005	2.1:+7686893	None:intergenic
GCACAGAGAGCAGCCGAGTG+GGG	0.647937	2.1:-7686671	MS.gene31579:CDS
GAGTGTGACGGAGAACGTAC+CGG	0.656780	2.1:+7686806	None:intergenic
GTTTCCGGTGACTCTTCTGA+CGG	0.659575	2.1:-7686527	MS.gene31579:CDS
CGGCACAGAGAGCAGCCGAG+TGG	0.661447	2.1:-7686673	MS.gene31579:CDS
GAAACTCTTGGAAACCCGCG+TGG	0.671188	2.1:+7686497	None:intergenic
GCTGAAACCGGGAAACCACA+AGG	0.672498	2.1:-7686629	MS.gene31579:CDS
TCTGACGGCGGTGATCCACG+CGG	0.687284	2.1:-7686512	MS.gene31579:CDS
GGATACTAATCCTGAAGACG+TGG	0.696029	2.1:-7685978	MS.gene31579:CDS
TCATTTCCGAGGGATCCTCG+AGG	0.749320	2.1:-7686869	MS.gene31579:CDS
AATACTTCGAGGGATCCTCG+AGG	0.764977	2.1:+7686854	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATTTATATTTACTATACA+TGG	+	chr2.1:7686699-7686718	None:intergenic	10.0%
!!	AATCACATGTAAAAAAAATA+TGG	-	chr2.1:7686572-7686591	MS.gene31579:CDS	15.0%
!!!	ATATAATTGTTTTTTGATTG+TGG	-	chr2.1:7686715-7686734	MS.gene31579:CDS	15.0%
!!!	TATAATTGTTTTTTGATTGT+GGG	-	chr2.1:7686716-7686735	MS.gene31579:CDS	15.0%
!!!	TTTTTGCTCAAAAAAAAAAA+AGG	+	chr2.1:7686468-7686487	None:intergenic	15.0%
!	AAAAGGAATTCTAATAACTC+TGG	-	chr2.1:7686196-7686215	MS.gene31579:intron	25.0%
!	TATACTTCAAAAGAAGTCAT+AGG	-	chr2.1:7686391-7686410	MS.gene31579:CDS	25.0%
!!	TTTTGTTCCATAAGTTAAGT+AGG	+	chr2.1:7686507-7686526	None:intergenic	25.0%
	AAGGTTACTGAATTACAAGA+AGG	-	chr2.1:7686838-7686857	MS.gene31579:CDS	30.0%
	ATCAAGTGTGTGTTGAAAAA+TGG	+	chr2.1:7686544-7686563	None:intergenic	30.0%
	TCAAGTGTGTGTTGAAAAAT+GGG	+	chr2.1:7686543-7686562	None:intergenic	30.0%
	TTACTGAATTACAAGAAGGA+TGG	-	chr2.1:7686842-7686861	MS.gene31579:CDS	30.0%
	TTCAACACACACTTGATATA+TGG	-	chr2.1:7686547-7686566	MS.gene31579:CDS	30.0%
!	GTTATTAGAATTCCTTTTGC+CGG	+	chr2.1:7686194-7686213	None:intergenic	30.0%
!!!	ATTGTTTTTTGATTGTGGGT+TGG	-	chr2.1:7686720-7686739	MS.gene31579:CDS	30.0%
!!!	TTGTTTTTTGATTGTGGGTT+GGG	-	chr2.1:7686721-7686740	MS.gene31579:CDS	30.0%
	AAGAAGTCATAGGAAGAAAC+TGG	-	chr2.1:7686401-7686420	MS.gene31579:CDS	35.0%
	ATAGTGAGAAGATTCCAGAA+TGG	+	chr2.1:7686872-7686891	None:intergenic	35.0%
	CAAGTGTGTGTTGAAAAATG+GGG	+	chr2.1:7686542-7686561	None:intergenic	35.0%
	CCATAAGTTAAGTAGGTACT+AGG	+	chr2.1:7686500-7686519	None:intergenic	35.0%
	CCTAGTACCTACTTAACTTA+TGG	-	chr2.1:7686497-7686516	MS.gene31579:CDS	35.0%
	TCAACAAACTTTCGTGGTTT+CGG	-	chr2.1:7686306-7686325	MS.gene31579:intron	35.0%
	TGCATACATAATCGGGTAAT+CGG	+	chr2.1:7686344-7686363	None:intergenic	35.0%
	TGTTGGATTGAATACTTCGA+GGG	+	chr2.1:7685909-7685928	None:intergenic	35.0%
	TTGTTGGATTGAATACTTCG+AGG	+	chr2.1:7685910-7685929	None:intergenic	35.0%
!	TGCTTTTCAACAAACTTTCG+TGG	-	chr2.1:7686300-7686319	MS.gene31579:intron	35.0%
!!	AAGATAAGGGAAGCATTAGA+AGG	-	chr2.1:7686797-7686816	MS.gene31579:CDS	35.0%
	AACAAGCAACTCAACTTCAC+CGG	-	chr2.1:7685925-7685944	MS.gene31579:CDS	40.0%
	AATAACTCTGGCAGAGTTTC+CGG	-	chr2.1:7686208-7686227	MS.gene31579:intron	40.0%
	ACAAAGACCATCATTTCCGA+GGG	-	chr2.1:7685871-7685890	MS.gene31579:CDS	40.0%
	ATGAAGTCACCAACACTTCA+TGG	-	chr2.1:7686023-7686042	MS.gene31579:intron	40.0%
	ATTGCCATCATCATCCTTGA+TGG	+	chr2.1:7686900-7686919	None:intergenic	40.0%
	CCTGAAAACATGGACAGAAA+CGG	-	chr2.1:7685970-7685989	MS.gene31579:CDS	40.0%
	CCTGCACTTGCATACATAAT+CGG	+	chr2.1:7686352-7686371	None:intergenic	40.0%
	CTGCACTTGCATACATAATC+GGG	+	chr2.1:7686351-7686370	None:intergenic	40.0%
	GAACAGAATGGAAAGTTCTC+CGG	-	chr2.1:7686172-7686191	MS.gene31579:intron	40.0%
	GAAGGTGGGAAAAGTTATTG+TGG	-	chr2.1:7686815-7686834	MS.gene31579:CDS	40.0%
	GCATACATAATCGGGTAATC+GGG	+	chr2.1:7686343-7686362	None:intergenic	40.0%
	GGCAATGTGCTCAAGTTAAT+TGG	-	chr2.1:7686914-7686933	MS.gene31579:CDS	40.0%
	GGTCTTTGTGATGAAGATGA+TGG	+	chr2.1:7685860-7685879	None:intergenic	40.0%
	GTGGGAAAAGTTATTGTGGA+AGG	-	chr2.1:7686819-7686838	MS.gene31579:CDS	40.0%
	GTTAATCAGGTGCAGAACTA+TGG	+	chr2.1:7686644-7686663	None:intergenic	40.0%
	TCTCACTATTTCACCCATCA+AGG	-	chr2.1:7686883-7686902	MS.gene31579:CDS	40.0%
	TGAAGACGTGGCAAAGATAA+GGG	-	chr2.1:7686784-7686803	MS.gene31579:CDS	40.0%
	TGATTAACCAGATATCACGC+AGG	-	chr2.1:7686656-7686675	MS.gene31579:CDS	40.0%
	TGCAGGTTTCTTCAACATGA+CGG	-	chr2.1:7686366-7686385	MS.gene31579:CDS	40.0%
	TGCGTGATATCTGGTTAATC+AGG	+	chr2.1:7686657-7686676	None:intergenic	40.0%
	TGTGTGTTGAAAAATGGGGT+AGG	+	chr2.1:7686538-7686557	None:intergenic	40.0%
	TTAAACACCTGCGTGATATC+TGG	+	chr2.1:7686666-7686685	None:intergenic	40.0%
	TTCAAATCCTCCGAAACTCT+TGG	+	chr2.1:7686268-7686287	None:intergenic	40.0%
	TTGCCATCATCATCCTTGAT+GGG	+	chr2.1:7686899-7686918	None:intergenic	40.0%
!!	ATAAGGGAAGCATTAGAAGG+TGG	-	chr2.1:7686800-7686819	MS.gene31579:CDS	40.0%
!!	TAAGGGAAGCATTAGAAGGT+GGG	-	chr2.1:7686801-7686820	MS.gene31579:CDS	40.0%
!!!	TTTTTGATTGTGGGTTGGGT+GGG	-	chr2.1:7686725-7686744	MS.gene31579:CDS	40.0%
!!!	TTTTTTGATTGTGGGTTGGG+TGG	-	chr2.1:7686724-7686743	MS.gene31579:CDS	40.0%
	AGAAGGATGGAACTCCATTC+TGG	-	chr2.1:7686855-7686874	MS.gene31579:CDS	45.0%
	AGTCACCAACACTTCATGGA+TGG	-	chr2.1:7686027-7686046	MS.gene31579:intron	45.0%
	ATGGAAAGTTCTCCGGCAAA+AGG	-	chr2.1:7686179-7686198	MS.gene31579:intron	45.0%
	CACAAAGACCATCATTTCCG+AGG	-	chr2.1:7685870-7685889	MS.gene31579:CDS	45.0%
	CACACTCACACCTGAAAACA+TGG	-	chr2.1:7685960-7685979	MS.gene31579:CDS	45.0%
	CACTTCATGGATGGCAATCA+AGG	-	chr2.1:7686036-7686055	MS.gene31579:intron	45.0%
	CATAATCGGGTAATCGGGTT+TGG	+	chr2.1:7686338-7686357	None:intergenic	45.0%
	CCGATTATGTATGCAAGTGC+AGG	-	chr2.1:7686349-7686368	MS.gene31579:intron	45.0%
	CTGAAGACGTGGCAAAGATA+AGG	-	chr2.1:7686783-7686802	MS.gene31579:CDS	45.0%
	CTTATCTTTGCCACGTCTTC+AGG	+	chr2.1:7686785-7686804	None:intergenic	45.0%
	GATTGCCATCCATGAAGTGT+TGG	+	chr2.1:7686035-7686054	None:intergenic	45.0%
	GCAATCAAGGAAGGAGAAAC+CGG	-	chr2.1:7686049-7686068	MS.gene31579:intron	45.0%
	GCAGGTTTCTTCAACATGAC+GGG	-	chr2.1:7686367-7686386	MS.gene31579:CDS	45.0%
	GGATACTAATCCTGAAGACG+TGG	-	chr2.1:7686772-7686791	MS.gene31579:CDS	45.0%
	TCACCCATCAAGGATGATGA+TGG	-	chr2.1:7686893-7686912	MS.gene31579:CDS	45.0%
	TCATGGATGGCAATCAAGGA+AGG	-	chr2.1:7686040-7686059	MS.gene31579:intron	45.0%
	TCCATTCTGTTCATCGTCAC+CGG	+	chr2.1:7686164-7686183	None:intergenic	45.0%
	TTTCTAACTCCCACTCCTTG+TGG	+	chr2.1:7686139-7686158	None:intergenic	45.0%
!	CCGTTTCTGTCCATGTTTTC+AGG	+	chr2.1:7685973-7685992	None:intergenic	45.0%
!	GGTGAAGTTGAGTTGCTTGT+TGG	+	chr2.1:7685926-7685945	None:intergenic	45.0%
!!	CAGTCGGTTTTTACAAGGTG+CGG	-	chr2.1:7686751-7686770	MS.gene31579:CDS	45.0%
!!!	GTTTTCAGGTGTGAGTGTGA+CGG	+	chr2.1:7685959-7685978	None:intergenic	45.0%
!!!	TTTTGATTGTGGGTTGGGTG+GGG	-	chr2.1:7686726-7686745	MS.gene31579:CDS	45.0%
	AATACTTCGAGGGATCCTCG+AGG	+	chr2.1:7685899-7685918	None:intergenic	50.0%
	ACGCGGGTTTCCAAGAGTTT+CGG	-	chr2.1:7686255-7686274	MS.gene31579:intron	50.0%
	CGCACCTTGTAAAAACCGAC+TGG	+	chr2.1:7686753-7686772	None:intergenic	50.0%
	CTCAGAACAGACGCTGAAAC+CGG	-	chr2.1:7686109-7686128	MS.gene31579:intron	50.0%
	GGAGTGGGAGTTAGAAACTC+CGG	-	chr2.1:7686142-7686161	MS.gene31579:intron	50.0%
	GTTTCCGGTGACTCTTCTGA+CGG	-	chr2.1:7686223-7686242	MS.gene31579:intron	50.0%
	TCAGAACAGACGCTGAAACC+GGG	-	chr2.1:7686110-7686129	MS.gene31579:intron	50.0%
	TCCGGTGACGATGAACAGAA+TGG	-	chr2.1:7686160-7686179	MS.gene31579:intron	50.0%
!!	AGTGTTGGTGACTTCATCGC+TGG	+	chr2.1:7686020-7686039	None:intergenic	50.0%
!!	GGGTCCAGTCGGTTTTTACA+AGG	-	chr2.1:7686746-7686765	MS.gene31579:CDS	50.0%
	AACCGGGAAACCACAAGGAG+TGG	-	chr2.1:7686126-7686145	MS.gene31579:intron	55.0%
	ACCGGGAAACCACAAGGAGT+GGG	-	chr2.1:7686127-7686146	MS.gene31579:intron	55.0%
	CGAGGATCCCTCGGAAATGA+TGG	+	chr2.1:7685881-7685900	None:intergenic	55.0%
	CGGGTTTCCAAGAGTTTCGG+AGG	-	chr2.1:7686258-7686277	MS.gene31579:intron	55.0%
	GAAACTCTTGGAAACCCGCG+TGG	+	chr2.1:7686256-7686275	None:intergenic	55.0%
	GCTGAAACCGGGAAACCACA+AGG	-	chr2.1:7686121-7686140	MS.gene31579:intron	55.0%
	TCATTTCCGAGGGATCCTCG+AGG	-	chr2.1:7685881-7685900	MS.gene31579:CDS	55.0%
	TCCCACTCCTTGTGGTTTCC+CGG	+	chr2.1:7686131-7686150	None:intergenic	55.0%
	TCTGAGCACCAAACCCCACT+CGG	+	chr2.1:7686095-7686114	None:intergenic	55.0%
!	GAGTGTGACGGAGAACGTAC+CGG	+	chr2.1:7685947-7685966	None:intergenic	55.0%
	ACCGCCGTCAGAAGAGTCAC+CGG	+	chr2.1:7686230-7686249	None:intergenic	60.0%
	AGGGATCCTCGAGGATCCCT+CGG	+	chr2.1:7685909-7685890	None:intergenic	60.0%
	TCCGGTGACTCTTCTGACGG+CGG	-	chr2.1:7686226-7686245	MS.gene31579:intron	60.0%
	GAGAGCAGCCGAGTGGGGTT+TGG	-	chr2.1:7686084-7686103	MS.gene31579:intron	65.0%
	GCACAGAGAGCAGCCGAGTG+GGG	-	chr2.1:7686079-7686098	MS.gene31579:intron	65.0%
	GGCACAGAGAGCAGCCGAGT+GGG	-	chr2.1:7686078-7686097	MS.gene31579:intron	65.0%
	TCTGACGGCGGTGATCCACG+CGG	-	chr2.1:7686238-7686257	MS.gene31579:intron	65.0%
!	TGGGTTGGGTGGGGTCCAGT+CGG	-	chr2.1:7686735-7686754	MS.gene31579:CDS	65.0%
	CGGCACAGAGAGCAGCCGAG+TGG	-	chr2.1:7686077-7686096	MS.gene31579:intron	70.0%
	CTCGGCTGCTCTCTGTGCCG+CGG	+	chr2.1:7686077-7686096	None:intergenic	70.0%
	CTGACGGCGGTGATCCACGC+GGG	-	chr2.1:7686239-7686258	MS.gene31579:intron	70.0%
	GGAAGGAGAAACCGGCGCCG+CGG	-	chr2.1:7686057-7686076	MS.gene31579:intron	70.0%
	TGCTCTCTGTGCCGCGGCGC+CGG	+	chr2.1:7686071-7686090	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	7685836	7686936	7685836	ID=MS.gene31579
chr2.1	mRNA	7685836	7686936	7685836	ID=MS.gene31579.t1;Parent=MS.gene31579
chr2.1	exon	7686350	7686936	7686350	ID=MS.gene31579.t1.exon1;Parent=MS.gene31579.t1
chr2.1	CDS	7686350	7686936	7686350	ID=cds.MS.gene31579.t1;Parent=MS.gene31579.t1
chr2.1	exon	7685836	7686018	7685836	ID=MS.gene31579.t1.exon2;Parent=MS.gene31579.t1
chr2.1	CDS	7685836	7686018	7685836	ID=cds.MS.gene31579.t1;Parent=MS.gene31579.t1

Gene Sequence

>MS.gene31579

ATGGAGCGTTTGAAGAAATCACCATCATCTTCATCACAAAGACCATCATTTCCGAGGGATCCTCGAGGATCCCTCGAAGTATTCAATCCAACAAGCAACTCAACTTCACCGGTACGTTCTCCGTCACACTCACACCTGAAAACATGGACAGAAACGGAAGAACAACACAAAGATTTCATTTCCAGCGATGAAGTCACCAACACTTCATGGATGGCAATCAAGGAAGGAGAAACCGGCGCCGCGGCACAGAGAGCAGCCGAGTGGGGTTTGGTGCTCAGAACAGACGCTGAAACCGGGAAACCACAAGGAGTGGGAGTTAGAAACTCCGGTGACGATGAACAGAATGGAAAGTTCTCCGGCAAAAGGAATTCTAATAACTCTGGCAGAGTTTCCGGTGACTCTTCTGACGGCGGTGATCCACGCGGGTTTCCAAGAGTTTCGGAGGATTTGAAAGATGCTTTATCTGCTTTTCAACAAACTTTCGTGGTTTCGGATGCTACCAAACCCGATTACCCGATTATGTATGCAAGTGCAGGTTTCTTCAACATGACGGGTTATACTTCAAAAGAAGTCATAGGAAGAAACTGGTAAAAAAATATCTTTACTGTTTATCTGTTAAAATATTACTACCTTTTTTTTTTTTGAGCAAAAAATATTACTACCTAGTACCTACTTAACTTATGGAACAAAAATGTTTAACCTACCCCATTTTTCAACACACACTTGATATATGGCTAATCACATGTAAAAAAAATATGGATAATTTAGTTGAGTAGATGATTAATCATCTACAAAACTAAATTAACCATAGTTCTGCACCTGATTAACCAGATATCACGCAGGTGTTTAAAGAAAGATGTCCATGTATAGTAAATATAAATATAATTGTTTTTTGATTGTGGGTTGGGTGGGGTCCAGTCGGTTTTTACAAGGTGCGGATACTAATCCTGAAGACGTGGCAAAGATAAGGGAAGCATTAGAAGGTGGGAAAAGTTATTGTGGAAGGTTACTGAATTACAAGAAGGATGGAACTCCATTCTGGAATCTTCTCACTATTTCACCCATCAAGGATGATGATGGCAATGTGCTCAAGTTAATTGG

Protein sequence

>MS.gene31579.t1

MERLKKSPSSSSQRPSFPRDPRGSLEVFNPTSNSTSPVRSPSHSHLKTWTETEEQHKDFISSDEVTNTSWMAIKEGETGAAAQRAAEWGLVLRTDAETGKPQGVGVRNSGDDEQNGKFSGKRNSNNSGRVSGDSSDGGDPRGFPRVSEDLKDALSAFQQTFVVSDATKPDYPIMYASAGFFNMTGYTSKEVIGRNCRFLQGADTNPEDVAKIREALEGGKSYCGRLLNYKKDGTPFWNLLTISPIKDDDGNVLKLI