Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068164.t1 | AAD34458.1 | 100 | 153 | 0 | 0 | 4 | 156 | 1 | 153 | 6.20E-78 | 300.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068164.t1 | Q9FHW7 | 77.1 | 170 | 23 | 1 | 3 | 156 | 2 | 171 | 7.9e-66 | 251.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068164.t1 | Q9XF99 | 100.0 | 153 | 0 | 0 | 4 | 156 | 1 | 153 | 4.4e-78 | 300.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049674 | MS.gene068164 | 0.811703 | 6.13E-51 | -1.69E-46 |
| MS.gene049951 | MS.gene068164 | 0.802065 | 6.69E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene068164 | 0.806808 | 6.87E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene068164 | 0.808743 | 2.67E-50 | -1.69E-46 |
| MS.gene050288 | MS.gene068164 | 0.822408 | 2.41E-53 | -1.69E-46 |
| MS.gene050317 | MS.gene068164 | 0.817361 | 3.43E-52 | -1.69E-46 |
| MS.gene050320 | MS.gene068164 | 0.87986 | 8.82E-70 | -1.69E-46 |
| MS.gene050437 | MS.gene068164 | 0.834559 | 2.79E-56 | -1.69E-46 |
| MS.gene050752 | MS.gene068164 | 0.873047 | 1.99E-67 | -1.69E-46 |
| MS.gene050838 | MS.gene068164 | 0.810498 | 1.12E-50 | -1.69E-46 |
| MS.gene050955 | MS.gene068164 | 0.843934 | 1.03E-58 | -1.69E-46 |
| MS.gene051003 | MS.gene068164 | 0.830375 | 3.04E-55 | -1.69E-46 |
| MS.gene051234 | MS.gene068164 | 0.803697 | 3.08E-49 | -1.69E-46 |
| MS.gene051245 | MS.gene068164 | 0.840605 | 7.86E-58 | -1.69E-46 |
| MS.gene051343 | MS.gene068164 | 0.805462 | 1.32E-49 | -1.69E-46 |
| MS.gene05134 | MS.gene068164 | 0.803943 | 2.74E-49 | -1.69E-46 |
| MS.gene051746 | MS.gene068164 | 0.817283 | 3.57E-52 | -1.69E-46 |
| MS.gene051748 | MS.gene068164 | 0.840489 | 8.43E-58 | -1.69E-46 |
| MS.gene051756 | MS.gene068164 | 0.809746 | 1.62E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene068164 | 0.82603 | 3.39E-54 | -1.69E-46 |
| MS.gene051975 | MS.gene068164 | 0.806881 | 6.63E-50 | -1.69E-46 |
| MS.gene052072 | MS.gene068164 | 0.806978 | 6.33E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene068164 | 0.817979 | 2.49E-52 | -1.69E-46 |
| MS.gene053058 | MS.gene068164 | 0.82881 | 7.31E-55 | -1.69E-46 |
| MS.gene053109 | MS.gene068164 | 0.859774 | 3.28E-63 | -1.69E-46 |
| MS.gene054403 | MS.gene068164 | 0.871232 | 7.99E-67 | -1.69E-46 |
| MS.gene05469 | MS.gene068164 | 0.84914 | 3.91E-60 | -1.69E-46 |
| MS.gene055311 | MS.gene068164 | 0.81869 | 1.72E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene068164 | 0.806072 | 9.82E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene068164 | 0.8276 | 1.43E-54 | -1.69E-46 |
| MS.gene057234 | MS.gene068164 | 0.826001 | 3.45E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene068164 | 0.862797 | 3.93E-64 | -1.69E-46 |
| MS.gene058238 | MS.gene068164 | 0.827937 | 1.19E-54 | -1.69E-46 |
| MS.gene059360 | MS.gene068164 | 0.812789 | 3.55E-51 | -1.69E-46 |
| MS.gene06010 | MS.gene068164 | 0.805909 | 1.06E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene068164 | 0.820224 | 7.68E-53 | -1.69E-46 |
| MS.gene060702 | MS.gene068164 | 0.848648 | 5.36E-60 | -1.69E-46 |
| MS.gene061145 | MS.gene068164 | 0.811199 | 7.89E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene98172 | MS.gene068164 | PPI |
| MS.gene92141 | MS.gene068164 | PPI |
| MS.gene068164 | MS.gene59610 | PPI |
| MS.gene25469 | MS.gene068164 | PPI |
| MS.gene068164 | MS.gene004511 | PPI |
| MS.gene068164 | MS.gene25471 | PPI |
| MS.gene068164 | MS.gene33667 | PPI |
| MS.gene068164 | MS.gene002824 | PPI |
| MS.gene068164 | MS.gene35971 | PPI |
| MS.gene047254 | MS.gene068164 | PPI |
| MS.gene068164 | MS.gene79720 | PPI |
| MS.gene068164 | MS.gene068281 | PPI |
| MS.gene068164 | MS.gene055864 | PPI |
| MS.gene24959 | MS.gene068164 | PPI |
| MS.gene00939 | MS.gene068164 | PPI |
| MS.gene068164 | MS.gene33411 | PPI |
| MS.gene79719 | MS.gene068164 | PPI |
| MS.gene068164 | MS.gene34691 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068164.t1 | MTR_5g022730 | 98.052 | 154 | 3 | 0 | 3 | 156 | 2 | 155 | 2.71e-110 | 310 |
| MS.gene068164.t1 | MTR_5g022710 | 96.104 | 154 | 6 | 0 | 3 | 156 | 2 | 155 | 1.92e-108 | 305 |
| MS.gene068164.t1 | MTR_7g021400 | 87.179 | 156 | 20 | 0 | 1 | 156 | 1 | 156 | 5.63e-93 | 266 |
| MS.gene068164.t1 | MTR_7g094150 | 86.538 | 156 | 21 | 0 | 1 | 156 | 1 | 156 | 1.58e-91 | 263 |
| MS.gene068164.t1 | MTR_7g094170 | 85.256 | 156 | 23 | 0 | 1 | 156 | 1 | 156 | 5.22e-90 | 259 |
| MS.gene068164.t1 | MTR_7g021380 | 83.333 | 156 | 26 | 0 | 1 | 156 | 1 | 156 | 4.29e-86 | 249 |
| MS.gene068164.t1 | MTR_3g110048 | 76.582 | 158 | 35 | 1 | 1 | 156 | 1 | 158 | 1.20e-85 | 248 |
| MS.gene068164.t1 | MTR_4g032400 | 78.846 | 156 | 32 | 1 | 2 | 156 | 8 | 163 | 1.40e-85 | 248 |
| MS.gene068164.t1 | MTR_7g094120 | 75.159 | 157 | 37 | 1 | 2 | 156 | 3 | 159 | 5.24e-84 | 244 |
| MS.gene068164.t1 | MTR_4g032450 | 73.885 | 157 | 40 | 1 | 1 | 156 | 19 | 175 | 6.38e-84 | 244 |
| MS.gene068164.t1 | MTR_8g056060 | 73.885 | 157 | 40 | 1 | 1 | 156 | 1 | 157 | 1.82e-79 | 232 |
| MS.gene068164.t1 | MTR_7g094280 | 72.152 | 158 | 42 | 2 | 1 | 156 | 1 | 158 | 6.79e-79 | 231 |
| MS.gene068164.t1 | MTR_5g085030 | 72.078 | 154 | 42 | 1 | 3 | 155 | 2 | 155 | 2.49e-77 | 227 |
| MS.gene068164.t1 | MTR_8g468480 | 69.677 | 155 | 46 | 1 | 3 | 156 | 2 | 156 | 5.67e-76 | 223 |
| MS.gene068164.t1 | MTR_4g032510 | 68.790 | 157 | 41 | 3 | 2 | 156 | 3 | 153 | 3.14e-71 | 211 |
| MS.gene068164.t1 | MTR_7g094310 | 68.987 | 158 | 37 | 3 | 1 | 156 | 1 | 148 | 4.14e-71 | 211 |
| MS.gene068164.t1 | MTR_4g032460 | 72.258 | 155 | 36 | 2 | 3 | 156 | 2 | 150 | 1.90e-70 | 209 |
| MS.gene068164.t1 | MTR_4g032500 | 66.667 | 156 | 45 | 2 | 2 | 156 | 3 | 152 | 1.07e-69 | 207 |
| MS.gene068164.t1 | MTR_4g033455 | 60.390 | 154 | 61 | 0 | 3 | 156 | 7 | 160 | 5.82e-65 | 196 |
| MS.gene068164.t1 | MTR_5g082340 | 66.452 | 155 | 40 | 2 | 3 | 156 | 2 | 145 | 3.85e-64 | 193 |
| MS.gene068164.t1 | MTR_4g017860 | 56.410 | 195 | 42 | 4 | 5 | 156 | 26 | 220 | 1.71e-63 | 194 |
| MS.gene068164.t1 | MTR_1g099870 | 84.314 | 102 | 14 | 1 | 57 | 156 | 2 | 103 | 6.75e-59 | 178 |
| MS.gene068164.t1 | MTR_4g021390 | 58.710 | 155 | 61 | 1 | 2 | 156 | 4 | 155 | 1.11e-58 | 179 |
| MS.gene068164.t1 | MTR_4g033460 | 60.000 | 140 | 56 | 0 | 3 | 142 | 7 | 146 | 2.42e-58 | 182 |
| MS.gene068164.t1 | MTR_4g033460 | 60.215 | 93 | 29 | 1 | 41 | 133 | 161 | 245 | 1.79e-30 | 110 |
| MS.gene068164.t1 | MTR_8g089730 | 69.919 | 123 | 20 | 1 | 34 | 156 | 1 | 106 | 2.73e-50 | 157 |
| MS.gene068164.t1 | MTR_7g094320 | 56.051 | 157 | 28 | 2 | 1 | 156 | 1 | 117 | 1.84e-48 | 152 |
| MS.gene068164.t1 | MTR_4g032420 | 61.417 | 127 | 34 | 3 | 31 | 156 | 38 | 150 | 6.05e-47 | 150 |
| MS.gene068164.t1 | MTR_7g006370 | 54.610 | 141 | 55 | 2 | 2 | 142 | 4 | 135 | 8.42e-44 | 141 |
| MS.gene068164.t1 | MTR_4g032470 | 51.370 | 146 | 27 | 2 | 12 | 156 | 32 | 134 | 1.13e-43 | 141 |
| MS.gene068164.t1 | MTR_7g094410 | 53.846 | 156 | 30 | 4 | 1 | 114 | 1 | 156 | 1.17e-41 | 137 |
| MS.gene068164.t1 | MTR_4g127240 | 66.990 | 103 | 28 | 4 | 40 | 140 | 26 | 124 | 1.90e-40 | 132 |
| MS.gene068164.t1 | MTR_4g131980 | 63.866 | 119 | 37 | 3 | 3 | 119 | 2 | 116 | 2.87e-40 | 132 |
| MS.gene068164.t1 | MTR_7g094360 | 47.093 | 172 | 38 | 4 | 1 | 119 | 1 | 172 | 7.87e-40 | 133 |
| MS.gene068164.t1 | MTR_8g056090 | 49.359 | 156 | 46 | 6 | 1 | 154 | 1 | 125 | 6.18e-37 | 124 |
| MS.gene068164.t1 | MTR_4g119422 | 48.980 | 147 | 68 | 2 | 2 | 146 | 227 | 368 | 2.71e-35 | 125 |
| MS.gene068164.t1 | MTR_1g112590 | 39.355 | 155 | 78 | 2 | 8 | 146 | 1 | 155 | 1.60e-32 | 113 |
| MS.gene068164.t1 | MTR_7g071200 | 71.014 | 69 | 19 | 1 | 89 | 156 | 4 | 72 | 1.16e-28 | 100 |
| MS.gene068164.t1 | MTR_2g066010 | 41.722 | 151 | 76 | 5 | 8 | 155 | 20 | 161 | 3.02e-27 | 101 |
| MS.gene068164.t1 | MTR_4g032520 | 44.444 | 144 | 38 | 4 | 29 | 154 | 1 | 120 | 3.41e-27 | 99.4 |
| MS.gene068164.t1 | MTR_2g065720 | 39.873 | 158 | 83 | 5 | 1 | 155 | 30 | 178 | 2.82e-26 | 99.0 |
| MS.gene068164.t1 | MTR_7g094350 | 50.459 | 109 | 32 | 2 | 1 | 109 | 1 | 87 | 2.93e-25 | 95.9 |
| MS.gene068164.t1 | MTR_7g094350 | 51.685 | 89 | 39 | 3 | 48 | 135 | 102 | 187 | 7.35e-20 | 82.0 |
| MS.gene068164.t1 | MTR_5g096950 | 37.736 | 159 | 78 | 4 | 1 | 155 | 1 | 142 | 1.71e-23 | 90.1 |
| MS.gene068164.t1 | MTR_4g032540 | 65.079 | 63 | 21 | 1 | 90 | 151 | 70 | 132 | 3.89e-23 | 88.6 |
| MS.gene068164.t1 | MTR_2g018980 | 34.667 | 150 | 85 | 3 | 8 | 156 | 3 | 140 | 2.67e-22 | 87.0 |
| MS.gene068164.t1 | MTR_1g042510 | 35.256 | 156 | 63 | 4 | 8 | 146 | 1 | 135 | 3.93e-22 | 89.7 |
| MS.gene068164.t1 | MTR_1g112570 | 36.691 | 139 | 63 | 4 | 8 | 128 | 1 | 132 | 1.16e-20 | 85.9 |
| MS.gene068164.t1 | MTR_5g078680 | 32.051 | 156 | 68 | 3 | 8 | 146 | 1 | 135 | 2.21e-19 | 82.0 |
| MS.gene068164.t1 | MTR_6g053620 | 36.806 | 144 | 81 | 5 | 8 | 146 | 17 | 155 | 3.46e-19 | 82.8 |
| MS.gene068164.t1 | MTR_6g053620 | 36.806 | 144 | 81 | 5 | 8 | 146 | 17 | 155 | 3.84e-19 | 82.8 |
| MS.gene068164.t1 | MTR_8g007195 | 34.286 | 140 | 80 | 4 | 10 | 143 | 13 | 146 | 2.24e-18 | 80.1 |
| MS.gene068164.t1 | MTR_7g077810 | 34.722 | 144 | 84 | 4 | 8 | 146 | 18 | 156 | 7.78e-18 | 79.0 |
| MS.gene068164.t1 | MTR_2g100825 | 32.667 | 150 | 92 | 4 | 1 | 146 | 4 | 148 | 5.04e-17 | 76.3 |
| MS.gene068164.t1 | MTR_2g100830 | 33.793 | 145 | 80 | 5 | 10 | 146 | 13 | 149 | 7.42e-17 | 75.9 |
| MS.gene068164.t1 | MTR_8g007185 | 32.877 | 146 | 87 | 4 | 8 | 147 | 11 | 151 | 8.12e-17 | 75.5 |
| MS.gene068164.t1 | MTR_8g007205 | 32.877 | 146 | 87 | 4 | 8 | 147 | 11 | 151 | 1.26e-16 | 75.1 |
| MS.gene068164.t1 | MTR_7g036780 | 34.043 | 141 | 82 | 6 | 1 | 135 | 1 | 136 | 1.98e-15 | 71.2 |
| MS.gene068164.t1 | MTR_4g131910 | 39.286 | 112 | 58 | 2 | 5 | 112 | 133 | 238 | 1.15e-14 | 69.3 |
| MS.gene068164.t1 | MTR_8g030670 | 30.345 | 145 | 91 | 3 | 10 | 149 | 20 | 159 | 1.98e-14 | 69.7 |
| MS.gene068164.t1 | MTR_3g061700 | 33.684 | 95 | 56 | 2 | 53 | 146 | 1 | 89 | 1.98e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068164.t1 | AT5G42190 | 77.059 | 170 | 23 | 1 | 3 | 156 | 2 | 171 | 2.43e-85 | 248 |
| MS.gene068164.t1 | AT1G20140 | 72.393 | 163 | 38 | 2 | 1 | 156 | 1 | 163 | 3.63e-83 | 242 |
| MS.gene068164.t1 | AT1G75950 | 77.987 | 159 | 29 | 1 | 4 | 156 | 2 | 160 | 3.74e-83 | 242 |
| MS.gene068164.t1 | AT2G25700 | 68.712 | 163 | 44 | 2 | 1 | 156 | 1 | 163 | 1.85e-78 | 230 |
| MS.gene068164.t1 | AT4G34210 | 70.130 | 154 | 42 | 2 | 4 | 156 | 2 | 152 | 1.78e-76 | 224 |
| MS.gene068164.t1 | AT4G34470 | 69.481 | 154 | 43 | 2 | 4 | 156 | 2 | 152 | 1.11e-75 | 223 |
| MS.gene068164.t1 | AT3G21860 | 67.097 | 155 | 45 | 3 | 4 | 156 | 2 | 152 | 4.85e-68 | 203 |
| MS.gene068164.t1 | AT3G21850 | 64.516 | 155 | 50 | 3 | 4 | 156 | 2 | 153 | 7.02e-67 | 200 |
| MS.gene068164.t1 | AT3G60010 | 64.331 | 157 | 47 | 3 | 5 | 156 | 2 | 154 | 2.10e-66 | 199 |
| MS.gene068164.t1 | AT2G03170 | 62.338 | 154 | 51 | 2 | 4 | 156 | 2 | 149 | 2.27e-63 | 191 |
| MS.gene068164.t1 | AT2G03190 | 55.422 | 166 | 61 | 3 | 4 | 156 | 2 | 167 | 7.23e-59 | 181 |
| MS.gene068164.t1 | AT3G60020 | 57.692 | 156 | 54 | 3 | 7 | 156 | 4 | 153 | 5.59e-57 | 175 |
| MS.gene068164.t1 | AT3G21830 | 56.494 | 154 | 63 | 2 | 4 | 156 | 2 | 152 | 1.04e-55 | 172 |
| MS.gene068164.t1 | AT2G03160 | 48.677 | 189 | 61 | 3 | 4 | 156 | 2 | 190 | 1.05e-55 | 174 |
| MS.gene068164.t1 | AT2G20160 | 54.902 | 153 | 63 | 2 | 4 | 155 | 2 | 149 | 2.07e-53 | 166 |
| MS.gene068164.t1 | AT3G25650 | 57.229 | 166 | 58 | 5 | 4 | 156 | 2 | 167 | 3.07e-53 | 167 |
| MS.gene068164.t1 | AT1G10230 | 56.774 | 155 | 64 | 2 | 4 | 155 | 2 | 156 | 4.76e-52 | 163 |
| MS.gene068164.t1 | AT3G21840 | 58.333 | 120 | 44 | 3 | 4 | 121 | 2 | 117 | 1.72e-37 | 125 |
| MS.gene068164.t1 | AT3G53060 | 66.279 | 86 | 22 | 3 | 29 | 113 | 1 | 80 | 8.25e-32 | 109 |
| MS.gene068164.t1 | AT3G61415 | 33.333 | 144 | 86 | 4 | 8 | 146 | 18 | 156 | 2.46e-16 | 74.7 |
| MS.gene068164.t1 | AT3G61415 | 33.333 | 144 | 86 | 4 | 8 | 146 | 18 | 156 | 2.57e-16 | 74.7 |
| MS.gene068164.t1 | AT2G45950 | 31.944 | 144 | 88 | 4 | 8 | 146 | 18 | 156 | 3.69e-16 | 74.3 |
| MS.gene068164.t1 | AT2G45950 | 31.944 | 144 | 88 | 4 | 8 | 146 | 18 | 156 | 3.69e-16 | 74.3 |
| MS.gene068164.t1 | AT2G45950 | 31.944 | 144 | 88 | 4 | 8 | 146 | 18 | 156 | 3.78e-16 | 74.3 |
| MS.gene068164.t1 | AT2G45950 | 31.944 | 144 | 88 | 4 | 8 | 146 | 18 | 156 | 3.78e-16 | 74.3 |
| MS.gene068164.t1 | AT2G45950 | 31.944 | 144 | 88 | 4 | 8 | 146 | 18 | 156 | 3.78e-16 | 74.3 |
Find 38 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAATATCAAGAGCTTGC+TGG | 0.358298 | 1.4:+37383812 | MS.gene068164:CDS |
| GGTCGATGAAGCGGTTGCTT+TGG | 0.364221 | 1.4:+37382078 | MS.gene068164:CDS |
| CAACGTTACCAGCAAGATCT+TGG | 0.386921 | 1.4:+37382162 | MS.gene068164:CDS |
| CGAGGATGAGCTTAAGTCTT+GGG | 0.409245 | 1.4:+37382246 | MS.gene068164:CDS |
| CGAGTTCGTCAAGGTTGATC+AGG | 0.435035 | 1.4:+37382273 | MS.gene068164:CDS |
| CACCTTCGCCAAGATCTTGC+TGG | 0.436666 | 1.4:-37382170 | None:intergenic |
| AATGTCTTGCGGATCTCCTC+TGG | 0.448832 | 1.4:-37383879 | None:intergenic |
| GACTTAAGCTCATCCTCGTT+AGG | 0.455978 | 1.4:-37382241 | None:intergenic |
| TGATCATGTCTGCAACAGTC+TGG | 0.458817 | 1.4:-37383845 | None:intergenic |
| AAGATCTTGCTGGTAACGTT+GGG | 0.477857 | 1.4:-37382160 | None:intergenic |
| TGATTTCTCACCTGAGGAAG+AGG | 0.505723 | 1.4:+37383914 | MS.gene068164:CDS |
| TGCTGGTAACGTTGGGAAGA+GGG | 0.508109 | 1.4:-37382153 | None:intergenic |
| TTGCTGGTAACGTTGGGAAG+AGG | 0.523250 | 1.4:-37382154 | None:intergenic |
| CAAGATCTTGCTGGTAACGT+TGG | 0.530853 | 1.4:-37382161 | None:intergenic |
| CGAACTTCCTCCTCTTCCTC+AGG | 0.533819 | 1.4:-37383924 | None:intergenic |
| GACTGTTGCAGACATGATCA+AGG | 0.534990 | 1.4:+37383848 | MS.gene068164:CDS |
| CTGATCAACCTTGACGAACT+CGG | 0.543997 | 1.4:-37382272 | None:intergenic |
| ACGAGGATGAGCTTAAGTCT+TGG | 0.544399 | 1.4:+37382245 | MS.gene068164:CDS |
| AGCATCATTCAAATGCCCAT+TGG | 0.546088 | 1.4:-37383956 | None:intergenic |
| AGGAAGAGGAGGAAGTTCGT+AGG | 0.548370 | 1.4:+37383928 | MS.gene068164:CDS |
| GGAAGAGGAGGAAGTTCGTA+GGG | 0.553401 | 1.4:+37383929 | MS.gene068164:CDS |
| AGTTCGTAGGGAAAACCAAT+GGG | 0.562143 | 1.4:+37383941 | MS.gene068164:CDS |
| AAGTTCGTAGGGAAAACCAA+TGG | 0.564773 | 1.4:+37383940 | MS.gene068164:CDS |
| TCTTGATGTTGAATGTCTTG+CGG | 0.592623 | 1.4:-37383890 | None:intergenic |
| TTGGGATTCCGAGTTCGTCA+AGG | 0.598524 | 1.4:+37382264 | MS.gene068164:CDS |
| GAAGATGATTGCGCTGACAG+TGG | 0.607194 | 1.4:+37382127 | MS.gene068164:CDS |
| GACCTTCGAGGTCGATGAAG+CGG | 0.610768 | 1.4:+37382069 | MS.gene068164:CDS |
| TTCCGACGGTGAGACCTTCG+AGG | 0.613590 | 1.4:+37382057 | MS.gene068164:CDS |
| ATCACTCTGAAGAGTTCCGA+CGG | 0.622850 | 1.4:+37382043 | MS.gene068164:CDS |
| AACCGCTTCATCGACCTCGA+AGG | 0.623664 | 1.4:-37382071 | None:intergenic |
| TACCAGCAAGATCTTGGCGA+AGG | 0.627734 | 1.4:+37382168 | MS.gene068164:CDS |
| TTTCTCACCTGAGGAAGAGG+AGG | 0.645844 | 1.4:+37383917 | MS.gene068164:CDS |
| CAAGAATGATTTCTCACCTG+AGG | 0.653595 | 1.4:+37383908 | MS.gene068164:CDS |
| ACTGTTGCAGACATGATCAA+GGG | 0.659883 | 1.4:+37383849 | MS.gene068164:CDS |
| GACCTCGAAGGTCTCACCGT+CGG | 0.662584 | 1.4:-37382059 | None:intergenic |
| TTGCAAGAAGCATGTTGACG+CGG | 0.672838 | 1.4:+37382201 | MS.gene068164:CDS |
| TGCTGAAGACAAACCTAACG+AGG | 0.677963 | 1.4:+37382228 | MS.gene068164:CDS |
| GATCAAGGGAAAGACACCAG+AGG | 0.742039 | 1.4:+37383863 | MS.gene068164:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATTATTAATAAAAAAT+AGG | - | chr1.4:37382459-37382478 | None:intergenic | 0.0% |
| !!! | TTTTTTATTAATAATAATAA+AGG | + | chr1.4:37382460-37382479 | MS.gene068164:intron | 0.0% |
| !! | ATTAATAATAATAAAGGATT+AGG | + | chr1.4:37382466-37382485 | MS.gene068164:intron | 10.0% |
| !! | TTAATAATAATAAAGGATTA+GGG | + | chr1.4:37382467-37382486 | MS.gene068164:intron | 10.0% |
| !!! | ATTTTATTTATGTTTTCATT+TGG | + | chr1.4:37382797-37382816 | MS.gene068164:intron | 10.0% |
| !!! | TTATTTAATTTAAATGGTTA+AGG | + | chr1.4:37383737-37383756 | MS.gene068164:intron | 10.0% |
| !! | TTATCGTTTATTATTTGATT+TGG | + | chr1.4:37382349-37382368 | MS.gene068164:intron | 15.0% |
| !!! | GGTTTATTCTTTTAAATTTT+AGG | + | chr1.4:37383496-37383515 | MS.gene068164:intron | 15.0% |
| !!! | TACTTAGGTTTTTTTAATAA+TGG | - | chr1.4:37383575-37383594 | None:intergenic | 15.0% |
| !!! | TATCGTTTATTATTTGATTT+GGG | + | chr1.4:37382350-37382369 | MS.gene068164:intron | 15.0% |
| !! | AAAGTTCTATAAAACATTGA+AGG | - | chr1.4:37383187-37383206 | None:intergenic | 20.0% |
| !! | AATAAAGAACATATAAAGCA+TGG | + | chr1.4:37383334-37383353 | MS.gene068164:intron | 20.0% |
| !! | GAAAAATCTGAATAGTTTAT+TGG | + | chr1.4:37382429-37382448 | MS.gene068164:intron | 20.0% |
| !! | TAAGGCTGATTAAATATTAT+TGG | + | chr1.4:37383755-37383774 | MS.gene068164:intron | 20.0% |
| !! | TTCATAAATCAATTGATGTT+CGG | - | chr1.4:37383278-37383297 | None:intergenic | 20.0% |
| !! | TTCTAATGTATTTGATTAAG+TGG | + | chr1.4:37383026-37383045 | MS.gene068164:intron | 20.0% |
| !! | TTGAAATTGAAAAAACTTCA+AGG | - | chr1.4:37382538-37382557 | None:intergenic | 20.0% |
| !!! | ATGTTTATGTTATGTTTTGT+TGG | + | chr1.4:37382883-37382902 | MS.gene068164:intron | 20.0% |
| !!! | ATTTCAATTTGTCTTTTGTT+TGG | + | chr1.4:37382551-37382570 | MS.gene068164:intron | 20.0% |
| !!! | TTGACATTGTTTTTCTTTTT+AGG | + | chr1.4:37383779-37383798 | MS.gene068164:intron | 20.0% |
| !!! | TTTCAATTTGTCTTTTGTTT+GGG | + | chr1.4:37382552-37382571 | MS.gene068164:intron | 20.0% |
| ! | GTATGATATCATTGTTTGTA+TGG | + | chr1.4:37383215-37383234 | MS.gene068164:intron | 25.0% |
| ! | TTAAAAAAACCTAAGTAGTC+TGG | + | chr1.4:37383578-37383597 | MS.gene068164:intron | 25.0% |
| !! | ACATTGTCTCAATATTTTGT+TGG | - | chr1.4:37383386-37383405 | None:intergenic | 25.0% |
| !! | TAATAAAGGATTAGGGTTTT+TGG | + | chr1.4:37382474-37382493 | MS.gene068164:intron | 25.0% |
| !!! | ATTAAGTGGTTCTAATTTTC+AGG | + | chr1.4:37383040-37383059 | MS.gene068164:intron | 25.0% |
| !!! | ATTTGATTTGGGTTTTGTTT+TGG | + | chr1.4:37382361-37382380 | MS.gene068164:intron | 25.0% |
| !!! | CATCTTGGTAAGTTTTTTTA+GGG | + | chr1.4:37382312-37382331 | MS.gene068164:intron | 25.0% |
| !!! | TCATCTTGGTAAGTTTTTTT+AGG | + | chr1.4:37382311-37382330 | MS.gene068164:intron | 25.0% |
| !!! | TTGTTTGGGTTGAATTTAAT+GGG | + | chr1.4:37382566-37382585 | MS.gene068164:intron | 25.0% |
| !!! | TTTGTTTGGGTTGAATTTAA+TGG | + | chr1.4:37382565-37382584 | MS.gene068164:intron | 25.0% |
| AACAAACAAACAGCCATATT+TGG | - | chr1.4:37383525-37383544 | None:intergenic | 30.0% | |
| AACAATAATCCAGACTACTT+AGG | - | chr1.4:37383590-37383609 | None:intergenic | 30.0% | |
| ACAAACAAACAGCCATATTT+GGG | - | chr1.4:37383524-37383543 | None:intergenic | 30.0% | |
| ACAACTTCTAGTAAATGTTG+TGG | + | chr1.4:37383474-37383493 | MS.gene068164:intron | 30.0% | |
| ACGCGTTTACTGAAAATTAA+GGG | + | chr1.4:37383065-37383084 | MS.gene068164:intron | 30.0% | |
| CAACAAGAAAACCCTAATTT+AGG | - | chr1.4:37382404-37382423 | None:intergenic | 30.0% | |
| CAACTTCTAGTAAATGTTGT+GGG | + | chr1.4:37383475-37383494 | MS.gene068164:intron | 30.0% | |
| TACGCGTTTACTGAAAATTA+AGG | + | chr1.4:37383064-37383083 | MS.gene068164:intron | 30.0% | |
| TTGTTGAGGAAATTGAAGTT+TGG | + | chr1.4:37383605-37383624 | MS.gene068164:intron | 30.0% | |
| ! | CAGAAACTTCTTCTAAAAAC+AGG | - | chr1.4:37383091-37383110 | None:intergenic | 30.0% |
| ! | CTAAATTAGGGTTTTCTTGT+TGG | + | chr1.4:37382402-37382421 | MS.gene068164:intron | 30.0% |
| !! | AAATTTTAGGAGCCCAAATA+TGG | + | chr1.4:37383509-37383528 | MS.gene068164:intron | 30.0% |
| !! | GTGTTGCTGAAAATTGTTTT+TGG | + | chr1.4:37383546-37383565 | MS.gene068164:intron | 30.0% |
| !! | TCTTTTTGTTCTTGAGTTGT+AGG | + | chr1.4:37382711-37382730 | MS.gene068164:intron | 30.0% |
| !!! | TTGTTTTGGAATGTTTGGTT+GGG | + | chr1.4:37382375-37382394 | MS.gene068164:intron | 30.0% |
| !!! | TTTGTTTTGGAATGTTTGGT+TGG | + | chr1.4:37382374-37382393 | MS.gene068164:intron | 30.0% |
| AAGCATGGACTTACTGTTAA+CGG | + | chr1.4:37383349-37383368 | MS.gene068164:intron | 35.0% | |
| AATAGCATATAGCCCAATAG+AGG | - | chr1.4:37382653-37382672 | None:intergenic | 35.0% | |
| AGAGAATTTGACATAGTGAG+TGG | - | chr1.4:37382856-37382875 | None:intergenic | 35.0% | |
| AGTAGTCTGGATTATTGTTG+AGG | + | chr1.4:37383591-37383610 | MS.gene068164:intron | 35.0% | |
| ATAAAAACAACACACTGCAG+TGG | - | chr1.4:37383721-37383740 | None:intergenic | 35.0% | |
| ATGAAATTCAACTAGCTGCA+AGG | + | chr1.4:37383293-37383312 | MS.gene068164:intron | 35.0% | |
| ATGCTATTGTGAAATCCGAA+AGG | + | chr1.4:37382761-37382780 | MS.gene068164:intron | 35.0% | |
| ATTCGTCGTGTTTGTTTGTT+TGG | + | chr1.4:37382930-37382949 | MS.gene068164:intron | 35.0% | |
| CACACAATCACTTAAAGAAG+TGG | + | chr1.4:37383003-37383022 | MS.gene068164:intron | 35.0% | |
| CGAGAGAATGTAAAATGCAA+TGG | - | chr1.4:37383690-37383709 | None:intergenic | 35.0% | |
| TCTTGATGTTGAATGTCTTG+CGG | - | chr1.4:37383893-37383912 | None:intergenic | 35.0% | |
| TGAAATTCAACTAGCTGCAA+GGG | + | chr1.4:37383294-37383313 | MS.gene068164:intron | 35.0% | |
| TGCTATTGTGAAATCCGAAA+GGG | + | chr1.4:37382762-37382781 | MS.gene068164:intron | 35.0% | |
| TGGAACTATAGTGAACTGAA+TGG | + | chr1.4:37383235-37383254 | MS.gene068164:intron | 35.0% | |
| ! | ACACTATCGATTAGAAGTGT+CGG | + | chr1.4:37383447-37383466 | MS.gene068164:intron | 35.0% |
| ! | TTGTACTATGTCACTTGTCA+AGG | + | chr1.4:37383415-37383434 | MS.gene068164:intron | 35.0% |
| !! | TACGCTTTTTGATCTCATCT+TGG | + | chr1.4:37382297-37382316 | MS.gene068164:CDS | 35.0% |
| !!! | AGGATTAGGGTTTTTGGTTT+TGG | + | chr1.4:37382480-37382499 | MS.gene068164:intron | 35.0% |
| !!! | GGATTAGGGTTTTTGGTTTT+GGG | + | chr1.4:37382481-37382500 | MS.gene068164:intron | 35.0% |
| !!! | GGGTTTTGTTTTGGAATGTT+TGG | + | chr1.4:37382370-37382389 | MS.gene068164:intron | 35.0% |
| !!! | TGTTTTGGAATGTTTGGTTG+GGG | + | chr1.4:37382376-37382395 | MS.gene068164:intron | 35.0% |
| AAAAAGATAGCCACAAGAGC+AGG | - | chr1.4:37382698-37382717 | None:intergenic | 40.0% | |
| AAGTTCGTAGGGAAAACCAA+TGG | + | chr1.4:37383940-37383959 | MS.gene068164:CDS | 40.0% | |
| ACTGTTGCAGACATGATCAA+GGG | + | chr1.4:37383849-37383868 | MS.gene068164:CDS | 40.0% | |
| AGTTCGTAGGGAAAACCAAT+GGG | + | chr1.4:37383941-37383960 | MS.gene068164:CDS | 40.0% | |
| ATCGCTGAATATTCCCCTTT+CGG | - | chr1.4:37382779-37382798 | None:intergenic | 40.0% | |
| ATTGTGCATTGTGCCTCTAT+TGG | + | chr1.4:37382637-37382656 | MS.gene068164:intron | 40.0% | |
| CAAGAATGATTTCTCACCTG+AGG | + | chr1.4:37383908-37383927 | MS.gene068164:CDS | 40.0% | |
| CTTGAATATCAAGAGCTTGC+TGG | + | chr1.4:37383812-37383831 | MS.gene068164:CDS | 40.0% | |
| GCTATTGTGAAATCCGAAAG+GGG | + | chr1.4:37382763-37382782 | MS.gene068164:intron | 40.0% | |
| TTGTGCATTGTGCCTCTATT+GGG | + | chr1.4:37382638-37382657 | MS.gene068164:intron | 40.0% | |
| ! | AAGATCTTGCTGGTAACGTT+GGG | - | chr1.4:37382163-37382182 | None:intergenic | 40.0% |
| ! | TATGTCACTTGTCAAGGTTC+TGG | + | chr1.4:37383421-37383440 | MS.gene068164:intron | 40.0% |
| !!! | GTTTTGGAATGTTTGGTTGG+GGG | + | chr1.4:37382377-37382396 | MS.gene068164:intron | 40.0% |
| ACGAGGATGAGCTTAAGTCT+TGG | + | chr1.4:37382245-37382264 | MS.gene068164:CDS | 45.0% | |
| CAACGTTACCAGCAAGATCT+TGG | + | chr1.4:37382162-37382181 | MS.gene068164:CDS | 45.0% | |
| CGAGGATGAGCTTAAGTCTT+GGG | + | chr1.4:37382246-37382265 | MS.gene068164:CDS | 45.0% | |
| CTGATCAACCTTGACGAACT+CGG | - | chr1.4:37382275-37382294 | None:intergenic | 45.0% | |
| GACTGTTGCAGACATGATCA+AGG | + | chr1.4:37383848-37383867 | MS.gene068164:CDS | 45.0% | |
| GACTTAAGCTCATCCTCGTT+AGG | - | chr1.4:37382244-37382263 | None:intergenic | 45.0% | |
| TGATCATGTCTGCAACAGTC+TGG | - | chr1.4:37383848-37383867 | None:intergenic | 45.0% | |
| TGATTTCTCACCTGAGGAAG+AGG | + | chr1.4:37383914-37383933 | MS.gene068164:CDS | 45.0% | |
| TGCTGAAGACAAACCTAACG+AGG | + | chr1.4:37382228-37382247 | MS.gene068164:CDS | 45.0% | |
| TGGTTGGGGGTCCTAAATTA+GGG | + | chr1.4:37382390-37382409 | MS.gene068164:intron | 45.0% | |
| TGTTGAATTGCCTGCTCTTG+TGG | + | chr1.4:37382685-37382704 | MS.gene068164:intron | 45.0% | |
| TTGGTTGGGGGTCCTAAATT+AGG | + | chr1.4:37382389-37382408 | MS.gene068164:intron | 45.0% | |
| ! | ATCACTCTGAAGAGTTCCGA+CGG | + | chr1.4:37382043-37382062 | MS.gene068164:CDS | 45.0% |
| ! | CAAGATCTTGCTGGTAACGT+TGG | - | chr1.4:37382164-37382183 | None:intergenic | 45.0% |
| ! | TTGCAAGAAGCATGTTGACG+CGG | + | chr1.4:37382201-37382220 | MS.gene068164:CDS | 45.0% |
| !!! | TTGTTTTTATTTAATTTAAA+TGG | + | chr1.4:37383731-37383750 | MS.gene068164:intron | 5.0% |
| AATGTCTTGCGGATCTCCTC+TGG | - | chr1.4:37383882-37383901 | None:intergenic | 50.0% | |
| AGGAAGAGGAGGAAGTTCGT+AGG | + | chr1.4:37383928-37383947 | MS.gene068164:CDS | 50.0% | |
| CAACTAGCTGCAAGGGATCT+TGG | + | chr1.4:37383301-37383320 | MS.gene068164:intron | 50.0% | |
| GATCAAGGGAAAGACACCAG+AGG | + | chr1.4:37383863-37383882 | MS.gene068164:CDS | 50.0% | |
| GGAAGAGGAGGAAGTTCGTA+GGG | + | chr1.4:37383929-37383948 | MS.gene068164:CDS | 50.0% | |
| TACCAGCAAGATCTTGGCGA+AGG | + | chr1.4:37382168-37382187 | MS.gene068164:CDS | 50.0% | |
| TTGGGATTCCGAGTTCGTCA+AGG | + | chr1.4:37382264-37382283 | MS.gene068164:CDS | 50.0% | |
| TTTCTCACCTGAGGAAGAGG+AGG | + | chr1.4:37383917-37383936 | MS.gene068164:CDS | 50.0% | |
| ! | CGAGTTCGTCAAGGTTGATC+AGG | + | chr1.4:37382273-37382292 | MS.gene068164:CDS | 50.0% |
| ! | GAAGATGATTGCGCTGACAG+TGG | + | chr1.4:37382127-37382146 | MS.gene068164:CDS | 50.0% |
| ! | TGCTGGTAACGTTGGGAAGA+GGG | - | chr1.4:37382156-37382175 | None:intergenic | 50.0% |
| ! | TTGCTGGTAACGTTGGGAAG+AGG | - | chr1.4:37382157-37382176 | None:intergenic | 50.0% |
| AACCGCTTCATCGACCTCGA+AGG | - | chr1.4:37382074-37382093 | None:intergenic | 55.0% | |
| CACCTTCGCCAAGATCTTGC+TGG | - | chr1.4:37382173-37382192 | None:intergenic | 55.0% | |
| CGAACTTCCTCCTCTTCCTC+AGG | - | chr1.4:37383927-37383946 | None:intergenic | 55.0% | |
| GACCTTCGAGGTCGATGAAG+CGG | + | chr1.4:37382069-37382088 | MS.gene068164:CDS | 55.0% | |
| GGTCGATGAAGCGGTTGCTT+TGG | + | chr1.4:37382078-37382097 | MS.gene068164:CDS | 55.0% | |
| GACCTCGAAGGTCTCACCGT+CGG | - | chr1.4:37382062-37382081 | None:intergenic | 60.0% | |
| TTCCGACGGTGAGACCTTCG+AGG | + | chr1.4:37382057-37382076 | MS.gene068164:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 37382022 | 37383974 | 37382022 | ID=MS.gene068164 |
| chr1.4 | mRNA | 37382022 | 37383974 | 37382022 | ID=MS.gene068164.t1;Parent=MS.gene068164 |
| chr1.4 | exon | 37382022 | 37382318 | 37382022 | ID=MS.gene068164.t1.exon1;Parent=MS.gene068164.t1 |
| chr1.4 | CDS | 37382022 | 37382318 | 37382022 | ID=cds.MS.gene068164.t1;Parent=MS.gene068164.t1 |
| chr1.4 | exon | 37383801 | 37383974 | 37383801 | ID=MS.gene068164.t1.exon2;Parent=MS.gene068164.t1 |
| chr1.4 | CDS | 37383801 | 37383974 | 37383801 | ID=cds.MS.gene068164.t1;Parent=MS.gene068164.t1 |
| Gene Sequence |
| Protein sequence |
