Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34299.t1 | XP_013454503.1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 1.40E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34299.t1 | Q9SUE0 | 86.2 | 87 | 12 | 0 | 7 | 93 | 127 | 213 | 3.0e-39 | 162.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34299.t1 | A0A072UFK1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 9.8e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene34299 | 0.846078 | 2.72E-59 | -1.69E-46 |
| MS.gene049173 | MS.gene34299 | 0.804227 | 2.39E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene34299 | 0.818418 | 1.98E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene34299 | 0.840157 | 1.03E-57 | -1.69E-46 |
| MS.gene050165 | MS.gene34299 | 0.837432 | 5.22E-57 | -1.69E-46 |
| MS.gene050333 | MS.gene34299 | 0.836761 | 7.74E-57 | -1.69E-46 |
| MS.gene050444 | MS.gene34299 | 0.814753 | 1.31E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene34299 | 0.836331 | 9.96E-57 | -1.69E-46 |
| MS.gene050612 | MS.gene34299 | 0.820884 | 5.42E-53 | -1.69E-46 |
| MS.gene050876 | MS.gene34299 | 0.802811 | 4.70E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene34299 | 0.806811 | 6.86E-50 | -1.69E-46 |
| MS.gene051317 | MS.gene34299 | 0.811691 | 6.17E-51 | -1.69E-46 |
| MS.gene051874 | MS.gene34299 | 0.80345 | 3.47E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene34299 | 0.800273 | 1.56E-48 | -1.69E-46 |
| MS.gene053284 | MS.gene34299 | 0.987578 | 9.12E-171 | -1.69E-46 |
| MS.gene053285 | MS.gene34299 | 0.9905 | 6.26E-183 | -1.69E-46 |
| MS.gene053916 | MS.gene34299 | 0.805161 | 1.53E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene34299 | 0.80638 | 8.46E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene34299 | 0.805573 | 1.25E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene34299 | 0.81325 | 2.81E-51 | -1.69E-46 |
| MS.gene055748 | MS.gene34299 | 0.840614 | 7.82E-58 | -1.69E-46 |
| MS.gene055751 | MS.gene34299 | 0.834789 | 2.45E-56 | -1.69E-46 |
| MS.gene056692 | MS.gene34299 | 0.801521 | 8.66E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene34299 | 0.812484 | 4.14E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene34299 | 0.803604 | 3.22E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene34299 | 0.827029 | 1.96E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene34299 | 0.821769 | 3.39E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene34299 | 0.809445 | 1.89E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene34299 | 0.819208 | 1.31E-52 | -1.69E-46 |
| MS.gene059677 | MS.gene34299 | 0.816084 | 6.64E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene34299 | 0.824801 | 6.63E-54 | -1.69E-46 |
| MS.gene06111 | MS.gene34299 | 0.813502 | 2.48E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene34299 | 0.858675 | 7.00E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34299.t1 | MTR_4g005520 | 95.789 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 4.30e-62 | 187 |
| MS.gene34299.t1 | MTR_3g105100 | 47.368 | 76 | 35 | 3 | 15 | 89 | 120 | 191 | 2.63e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34299.t1 | AT4G27990 | 85.227 | 88 | 13 | 0 | 7 | 94 | 127 | 214 | 8.55e-52 | 162 |
| MS.gene34299.t1 | AT3G07430 | 83.908 | 87 | 14 | 0 | 7 | 93 | 141 | 227 | 5.25e-51 | 160 |
| MS.gene34299.t1 | AT5G21920 | 50.000 | 76 | 33 | 3 | 15 | 89 | 133 | 204 | 8.24e-15 | 67.4 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAACCATTGTCTGCAATT+AGG | 0.283838 | 4.3:+88901720 | MS.gene34299:CDS |
| ACAACCATTGTCTGCAATTA+GGG | 0.307081 | 4.3:+88901721 | MS.gene34299:CDS |
| ACAGTGGTGTGTTGTTGGTT+AGG | 0.327645 | 4.3:+88901660 | MS.gene34299:CDS |
| ATCCTTATCTAAGTTTGTTT+AGG | 0.329465 | 4.3:+88901753 | MS.gene34299:CDS |
| CAGTGGTGTGTTGTTGGTTA+GGG | 0.363509 | 4.3:+88901661 | MS.gene34299:CDS |
| TAGCGGCGGGGTTGAAGAAA+TGG | 0.403666 | 4.3:+88901627 | MS.gene34299:CDS |
| TATTTACAGTGGTGTGTTGT+TGG | 0.416695 | 4.3:+88901655 | MS.gene34299:CDS |
| GACGGTGAACACGCCGTTGA+CGG | 0.439588 | 4.3:+88901601 | MS.gene34299:CDS |
| CCGTTGACGGTTGTAGCGGC+GGG | 0.441420 | 4.3:+88901614 | MS.gene34299:CDS |
| TGTCTTTCCCATGGAATGTT+GGG | 0.454889 | 4.3:-88901701 | None:intergenic |
| GTTGGTTTCCCAACATTCCA+TGG | 0.512936 | 4.3:+88901693 | MS.gene34299:CDS |
| GACAATGGTTGTCTTTCCCA+TGG | 0.515980 | 4.3:-88901710 | None:intergenic |
| TTCCTAAACAAACTTAGATA+AGG | 0.528049 | 4.3:-88901755 | None:intergenic |
| GCCGTTGACGGTTGTAGCGG+CGG | 0.535668 | 4.3:+88901613 | MS.gene34299:CDS |
| TTGGTTTCCCAACATTCCAT+GGG | 0.548737 | 4.3:+88901694 | MS.gene34299:CDS |
| CCCGCCGCTACAACCGTCAA+CGG | 0.573983 | 4.3:-88901614 | None:intergenic |
| TTGTCTTTCCCATGGAATGT+TGG | 0.574451 | 4.3:-88901702 | None:intergenic |
| AAAACAGCAAAAGCAAGAAG+AGG | 0.574512 | 4.3:-88901815 | None:intergenic |
| CACGCCGTTGACGGTTGTAG+CGG | 0.575214 | 4.3:+88901610 | MS.gene34299:CDS |
| CGTTGACGGTTGTAGCGGCG+GGG | 0.610796 | 4.3:+88901615 | MS.gene34299:CDS |
| GGCGGGGTTGAAGAAATGGT+TGG | 0.623671 | 4.3:+88901631 | MS.gene34299:CDS |
| AAATGGTTGGATATTTACAG+TGG | 0.642279 | 4.3:+88901644 | MS.gene34299:CDS |
| AGATCCCTAATTGCAGACAA+TGG | 0.662621 | 4.3:-88901725 | None:intergenic |
| GGATCTAAACAGGAACAGTG+AGG | 0.722011 | 4.3:-88901856 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCCTAAACAAACTTAGATA+AGG | - | chr4.3:88901758-88901777 | None:intergenic | 25.0% |
| !! | ATCCTTATCTAAGTTTGTTT+AGG | + | chr4.3:88901753-88901772 | MS.gene34299:CDS | 25.0% |
| !!! | ATATCAAGAGTATCAAAAAC+AGG | - | chr4.3:88901791-88901810 | None:intergenic | 25.0% |
| AAATGGTTGGATATTTACAG+TGG | + | chr4.3:88901644-88901663 | MS.gene34299:CDS | 30.0% | |
| AAAACAGCAAAAGCAAGAAG+AGG | - | chr4.3:88901818-88901837 | None:intergenic | 35.0% | |
| ACAACCATTGTCTGCAATTA+GGG | + | chr4.3:88901721-88901740 | MS.gene34299:CDS | 35.0% | |
| TATTTACAGTGGTGTGTTGT+TGG | + | chr4.3:88901655-88901674 | MS.gene34299:CDS | 35.0% | |
| !! | TCAAGAGTATCAAAAACAGG+TGG | - | chr4.3:88901788-88901807 | None:intergenic | 35.0% |
| !!! | CTTCTTGCTTTTGCTGTTTT+GGG | + | chr4.3:88901818-88901837 | MS.gene34299:CDS | 35.0% |
| !!! | TCTTCTTGCTTTTGCTGTTT+TGG | + | chr4.3:88901817-88901836 | MS.gene34299:CDS | 35.0% |
| AGATCCCTAATTGCAGACAA+TGG | - | chr4.3:88901728-88901747 | None:intergenic | 40.0% | |
| GACAACCATTGTCTGCAATT+AGG | + | chr4.3:88901720-88901739 | MS.gene34299:CDS | 40.0% | |
| TGTCTTTCCCATGGAATGTT+GGG | - | chr4.3:88901704-88901723 | None:intergenic | 40.0% | |
| TTGGTTTCCCAACATTCCAT+GGG | + | chr4.3:88901694-88901713 | MS.gene34299:CDS | 40.0% | |
| TTGTCTTTCCCATGGAATGT+TGG | - | chr4.3:88901705-88901724 | None:intergenic | 40.0% | |
| !!! | TGGTTAGGGTTTTGTTGAGT+TGG | + | chr4.3:88901675-88901694 | MS.gene34299:CDS | 40.0% |
| GACAATGGTTGTCTTTCCCA+TGG | - | chr4.3:88901713-88901732 | None:intergenic | 45.0% | |
| ! | ACAGTGGTGTGTTGTTGGTT+AGG | + | chr4.3:88901660-88901679 | MS.gene34299:CDS | 45.0% |
| ! | CAGTGGTGTGTTGTTGGTTA+GGG | + | chr4.3:88901661-88901680 | MS.gene34299:CDS | 45.0% |
| ! | GTTGGTTTCCCAACATTCCA+TGG | + | chr4.3:88901693-88901712 | MS.gene34299:CDS | 45.0% |
| !! | CCCGTCCTAGTCTTTTTCAT+AGG | - | chr4.3:88901581-88901600 | None:intergenic | 45.0% |
| GAAAAAGACTAGGACGGGGA+CGG | + | chr4.3:88901583-88901602 | MS.gene34299:CDS | 50.0% | |
| !! | GCTGTTTTGGGTACTCTTGC+TGG | + | chr4.3:88901830-88901849 | MS.gene34299:CDS | 50.0% |
| GGCGGGGTTGAAGAAATGGT+TGG | + | chr4.3:88901631-88901650 | MS.gene34299:CDS | 55.0% | |
| TAGCGGCGGGGTTGAAGAAA+TGG | + | chr4.3:88901627-88901646 | MS.gene34299:CDS | 55.0% | |
| CACGCCGTTGACGGTTGTAG+CGG | + | chr4.3:88901610-88901629 | MS.gene34299:CDS | 60.0% | |
| GACGGTGAACACGCCGTTGA+CGG | + | chr4.3:88901601-88901620 | MS.gene34299:CDS | 60.0% | |
| CCCGCCGCTACAACCGTCAA+CGG | - | chr4.3:88901617-88901636 | None:intergenic | 65.0% | |
| CCGTTGACGGTTGTAGCGGC+GGG | + | chr4.3:88901614-88901633 | MS.gene34299:CDS | 65.0% | |
| CGTTGACGGTTGTAGCGGCG+GGG | + | chr4.3:88901615-88901634 | MS.gene34299:CDS | 65.0% | |
| GCCGTTGACGGTTGTAGCGG+CGG | + | chr4.3:88901613-88901632 | MS.gene34299:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 88901581 | 88901868 | 88901581 | ID=MS.gene34299 |
| chr4.3 | mRNA | 88901581 | 88901868 | 88901581 | ID=MS.gene34299.t1;Parent=MS.gene34299 |
| chr4.3 | exon | 88901581 | 88901868 | 88901581 | ID=MS.gene34299.t1.exon1;Parent=MS.gene34299.t1 |
| chr4.3 | CDS | 88901581 | 88901868 | 88901581 | ID=cds.MS.gene34299.t1;Parent=MS.gene34299.t1 |
| Gene Sequence |
| Protein sequence |
