Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34314.t1 | XP_013454489.1 | 100 | 70 | 0 | 0 | 1 | 70 | 72 | 141 | 3.40E-28 | 133.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34314.t1 | Q8GWH5 | 81.4 | 70 | 13 | 0 | 1 | 70 | 57 | 126 | 1.5e-24 | 112.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34314.t1 | A0A072UFI9 | 100.0 | 70 | 0 | 0 | 1 | 70 | 72 | 141 | 2.5e-28 | 133.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049322 | MS.gene34314 | -0.809632 | 1.72E-50 | -1.69E-46 |
| MS.gene050114 | MS.gene34314 | -0.8011 | 1.06E-48 | -1.69E-46 |
| MS.gene050317 | MS.gene34314 | -0.81823 | 2.19E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene34314 | 0.831852 | 1.32E-55 | -1.69E-46 |
| MS.gene050762 | MS.gene34314 | -0.851602 | 7.98E-61 | -1.69E-46 |
| MS.gene050763 | MS.gene34314 | -0.852018 | 6.08E-61 | -1.69E-46 |
| MS.gene051245 | MS.gene34314 | -0.818027 | 2.43E-52 | -1.69E-46 |
| MS.gene052970 | MS.gene34314 | 0.947338 | 8.16E-106 | -1.69E-46 |
| MS.gene054379 | MS.gene34314 | -0.835888 | 1.29E-56 | -1.69E-46 |
| MS.gene055413 | MS.gene34314 | -0.825664 | 4.14E-54 | -1.69E-46 |
| MS.gene055441 | MS.gene34314 | -0.803159 | 3.98E-49 | -1.69E-46 |
| MS.gene056400 | MS.gene34314 | -0.802309 | 5.96E-49 | -1.69E-46 |
| MS.gene056552 | MS.gene34314 | -0.814269 | 1.68E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene34314 | -0.828751 | 7.55E-55 | -1.69E-46 |
| MS.gene057452 | MS.gene34314 | 0.802034 | 6.80E-49 | -1.69E-46 |
| MS.gene058182 | MS.gene34314 | -0.831403 | 1.70E-55 | -1.69E-46 |
| MS.gene058399 | MS.gene34314 | -0.804447 | 2.15E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene34314 | -0.855438 | 6.31E-62 | -1.69E-46 |
| MS.gene060297 | MS.gene34314 | -0.820662 | 6.09E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCTTGGAAGACTGGCTT+TGG | 0.289145 | 4.3:+89182664 | MS.gene34314:CDS |
| CTTTATGCATTATCTGCTCT+TGG | 0.374994 | 4.3:+89182650 | MS.gene34314:CDS |
| GTCTTGAGGGTTGCTGCTAC+TGG | 0.407570 | 4.3:+89182299 | MS.gene34314:CDS |
| GCTCTTGGAAGACTGGCTTT+GGG | 0.418794 | 4.3:+89182665 | MS.gene34314:CDS |
| TGCCACTCAAGGGGATCAAC+AGG | 0.434496 | 4.3:-89182209 | None:intergenic |
| TGCCGCTGAATCTGTCTTGA+GGG | 0.443932 | 4.3:+89182286 | MS.gene34314:CDS |
| CAAGAGCAGATAATGCATAA+AGG | 0.448013 | 4.3:-89182649 | None:intergenic |
| ATTATCTGCTCTTGGAAGAC+TGG | 0.464565 | 4.3:+89182658 | MS.gene34314:CDS |
| GGTATTACTATCGCCTATGC+TGG | 0.488512 | 4.3:+89182686 | MS.gene34314:CDS |
| GCTTCTTTCGCTAATACTGT+CGG | 0.494988 | 4.3:+89182263 | MS.gene34314:CDS |
| TTGAGATATTTGCCACTCAA+GGG | 0.504422 | 4.3:-89182219 | None:intergenic |
| CTTGAGATATTTGCCACTCA+AGG | 0.517790 | 4.3:-89182220 | None:intergenic |
| GTGCCGCTGAATCTGTCTTG+AGG | 0.538110 | 4.3:+89182285 | MS.gene34314:CDS |
| AGAAAGGTACGAACCAGCAT+AGG | 0.589035 | 4.3:-89182699 | None:intergenic |
| TGAGATATTTGCCACTCAAG+GGG | 0.608929 | 4.3:-89182218 | None:intergenic |
| TTCCTGTTGATCCCCTTGAG+TGG | 0.613108 | 4.3:+89182207 | MS.gene34314:CDS |
| AACCCTCAAGACAGATTCAG+CGG | 0.632761 | 4.3:-89182288 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAGTTAGAAATATAAAAA+AGG | - | chr4.3:89182595-89182614 | None:intergenic | 15.0% |
| !!! | TGACAAAAGACTCTTTTTTA+AGG | + | chr4.3:89182325-89182344 | MS.gene34314:CDS | 25.0% |
| AAAGAATGACCTGTAATCAT+TGG | - | chr4.3:89182628-89182647 | None:intergenic | 30.0% | |
| TGAAAAACTGAAATAGACAC+CGG | + | chr4.3:89182389-89182408 | MS.gene34314:intron | 30.0% | |
| ACATCTTCAATCTCAAGTGT+CGG | + | chr4.3:89182512-89182531 | MS.gene34314:intron | 35.0% | |
| ACTCATCTACCAATGATTAC+AGG | + | chr4.3:89182616-89182635 | MS.gene34314:intron | 35.0% | |
| CAAGAGCAGATAATGCATAA+AGG | - | chr4.3:89182652-89182671 | None:intergenic | 35.0% | |
| CTTTATGCATTATCTGCTCT+TGG | + | chr4.3:89182650-89182669 | MS.gene34314:CDS | 35.0% | |
| TTGAGATATTTGCCACTCAA+GGG | - | chr4.3:89182222-89182241 | None:intergenic | 35.0% | |
| ATTATCTGCTCTTGGAAGAC+TGG | + | chr4.3:89182658-89182677 | MS.gene34314:CDS | 40.0% | |
| CTTGAGATATTTGCCACTCA+AGG | - | chr4.3:89182223-89182242 | None:intergenic | 40.0% | |
| GCTTCTTTCGCTAATACTGT+CGG | + | chr4.3:89182263-89182282 | MS.gene34314:CDS | 40.0% | |
| TGAGATATTTGCCACTCAAG+GGG | - | chr4.3:89182221-89182240 | None:intergenic | 40.0% | |
| AACCCTCAAGACAGATTCAG+CGG | - | chr4.3:89182291-89182310 | None:intergenic | 45.0% | |
| ACTGAAATAGACACCGGACA+TGG | + | chr4.3:89182395-89182414 | MS.gene34314:intron | 45.0% | |
| TGTTATGTTCGGCTGACACT+TGG | + | chr4.3:89182539-89182558 | MS.gene34314:intron | 45.0% | |
| ! | GTGTCGGTCAGTGTTATGTT+CGG | + | chr4.3:89182528-89182547 | MS.gene34314:intron | 45.0% |
| !! | CACTTGGTGTTTCGTAAGTG+TGG | + | chr4.3:89182555-89182574 | MS.gene34314:intron | 45.0% |
| TGAGTCGTTGTGTCCATGTC+CGG | - | chr4.3:89182411-89182430 | None:intergenic | 50.0% | |
| TGCCGCTGAATCTGTCTTGA+GGG | + | chr4.3:89182286-89182305 | MS.gene34314:CDS | 50.0% | |
| TTCCTGTTGATCCCCTTGAG+TGG | + | chr4.3:89182207-89182226 | MS.gene34314:CDS | 50.0% | |
| !! | GCTCTTGGAAGACTGGCTTT+GGG | + | chr4.3:89182665-89182684 | MS.gene34314:CDS | 50.0% |
| !! | TGCTCTTGGAAGACTGGCTT+TGG | + | chr4.3:89182664-89182683 | MS.gene34314:CDS | 50.0% |
| GTCTTGAGGGTTGCTGCTAC+TGG | + | chr4.3:89182299-89182318 | MS.gene34314:CDS | 55.0% | |
| TGCCACTCAAGGGGATCAAC+AGG | - | chr4.3:89182212-89182231 | None:intergenic | 55.0% | |
| ! | GTGCCGCTGAATCTGTCTTG+AGG | + | chr4.3:89182285-89182304 | MS.gene34314:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 89182205 | 89182707 | 89182205 | ID=MS.gene34314 |
| chr4.3 | mRNA | 89182205 | 89182707 | 89182205 | ID=MS.gene34314.t1;Parent=MS.gene34314 |
| chr4.3 | exon | 89182205 | 89182346 | 89182205 | ID=MS.gene34314.t1.exon1;Parent=MS.gene34314.t1 |
| chr4.3 | CDS | 89182205 | 89182346 | 89182205 | ID=cds.MS.gene34314.t1;Parent=MS.gene34314.t1 |
| chr4.3 | exon | 89182638 | 89182707 | 89182638 | ID=MS.gene34314.t1.exon2;Parent=MS.gene34314.t1 |
| chr4.3 | CDS | 89182638 | 89182707 | 89182638 | ID=cds.MS.gene34314.t1;Parent=MS.gene34314.t1 |
| Gene Sequence |
| Protein sequence |
