Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36866.t1 | XP_013444912.1 | 96.1 | 179 | 7 | 0 | 1 | 179 | 1 | 179 | 9.20E-86 | 326.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36866.t1 | Q58FX0 | 35.1 | 202 | 100 | 5 | 3 | 177 | 8 | 205 | 3.0e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36866.t1 | A0A072TNG6 | 96.1 | 179 | 7 | 0 | 1 | 179 | 1 | 179 | 6.6e-86 | 326.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene36866 | 0.807065 | 6.06E-50 | -1.69E-46 |
| MS.gene049413 | MS.gene36866 | 0.820942 | 5.25E-53 | -1.69E-46 |
| MS.gene04998 | MS.gene36866 | 0.860489 | 1.99E-63 | -1.69E-46 |
| MS.gene050961 | MS.gene36866 | 0.815138 | 1.08E-51 | -1.69E-46 |
| MS.gene051226 | MS.gene36866 | 0.809627 | 1.72E-50 | -1.69E-46 |
| MS.gene051959 | MS.gene36866 | 0.802755 | 4.83E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene36866 | 0.813637 | 2.31E-51 | -1.69E-46 |
| MS.gene054178 | MS.gene36866 | 0.800286 | 1.55E-48 | -1.69E-46 |
| MS.gene054892 | MS.gene36866 | 0.866371 | 2.99E-65 | -1.69E-46 |
| MS.gene054893 | MS.gene36866 | 0.860616 | 1.82E-63 | -1.69E-46 |
| MS.gene055229 | MS.gene36866 | 0.810811 | 9.57E-51 | -1.69E-46 |
| MS.gene05624 | MS.gene36866 | 0.813978 | 1.95E-51 | -1.69E-46 |
| MS.gene057093 | MS.gene36866 | 0.805996 | 1.02E-49 | -1.69E-46 |
| MS.gene057155 | MS.gene36866 | 0.804568 | 2.03E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene36866 | -0.815184 | 1.05E-51 | -1.69E-46 |
| MS.gene06023 | MS.gene36866 | 0.801484 | 8.81E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene36866 | 0.805045 | 1.61E-49 | -1.69E-46 |
| MS.gene060412 | MS.gene36866 | 0.814868 | 1.24E-51 | -1.69E-46 |
| MS.gene060413 | MS.gene36866 | 0.828281 | 9.81E-55 | -1.69E-46 |
| MS.gene06061 | MS.gene36866 | 0.800794 | 1.22E-48 | -1.69E-46 |
| MS.gene061047 | MS.gene36866 | 0.839732 | 1.33E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36866.t1 | MTR_8g432400 | 96.648 | 179 | 6 | 0 | 1 | 179 | 1 | 179 | 4.31e-128 | 357 |
| MS.gene36866.t1 | MTR_8g432390 | 91.061 | 179 | 16 | 0 | 1 | 179 | 1 | 179 | 7.97e-110 | 311 |
| MS.gene36866.t1 | MTR_8g432350 | 89.385 | 179 | 19 | 0 | 1 | 179 | 1 | 179 | 3.01e-107 | 304 |
| MS.gene36866.t1 | MTR_8g432370 | 89.385 | 179 | 18 | 1 | 1 | 179 | 1 | 178 | 2.78e-105 | 299 |
| MS.gene36866.t1 | MTR_7g076000 | 54.396 | 182 | 63 | 5 | 1 | 178 | 1 | 166 | 2.07e-58 | 180 |
| MS.gene36866.t1 | MTR_8g432470 | 38.043 | 184 | 96 | 6 | 2 | 175 | 5 | 180 | 9.20e-29 | 105 |
| MS.gene36866.t1 | MTR_8g432450 | 36.517 | 178 | 97 | 4 | 2 | 169 | 5 | 176 | 3.04e-27 | 101 |
| MS.gene36866.t1 | MTR_8g432440 | 35.870 | 184 | 100 | 6 | 2 | 175 | 5 | 180 | 6.01e-27 | 100 |
| MS.gene36866.t1 | MTR_8g432420 | 35.870 | 184 | 100 | 6 | 2 | 175 | 5 | 180 | 6.01e-27 | 100 |
| MS.gene36866.t1 | MTR_8g432460 | 36.066 | 183 | 100 | 6 | 2 | 175 | 5 | 179 | 3.38e-25 | 96.3 |
| MS.gene36866.t1 | MTR_8g432490 | 34.973 | 183 | 102 | 6 | 2 | 175 | 5 | 179 | 6.90e-24 | 92.8 |
| MS.gene36866.t1 | MTR_3g064780 | 34.973 | 183 | 102 | 7 | 2 | 175 | 5 | 179 | 4.41e-22 | 89.7 |
| MS.gene36866.t1 | MTR_8g432480 | 32.749 | 171 | 105 | 5 | 2 | 171 | 7 | 168 | 5.95e-21 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36866.t1 | AT2G45760 | 35.294 | 204 | 101 | 5 | 1 | 177 | 6 | 205 | 4.43e-35 | 122 |
| MS.gene36866.t1 | AT3G61190 | 37.766 | 188 | 97 | 6 | 1 | 179 | 16 | 192 | 1.15e-29 | 108 |
| MS.gene36866.t1 | AT3G61190 | 37.766 | 188 | 97 | 6 | 1 | 179 | 16 | 192 | 1.15e-29 | 108 |
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAATATCATATGTTTCTT+TGG | 0.263645 | 8.4:+64144296 | None:intergenic |
| ATAATAGAGTTAGGAGAAAT+GGG | 0.321912 | 8.4:-64144480 | MS.gene36866:CDS |
| GATAATAGAGTTAGGAGAAA+TGG | 0.337385 | 8.4:-64144481 | MS.gene36866:CDS |
| GTGAGTGGAGTTCCGGTAAC+CGG | 0.370119 | 8.4:-64144388 | MS.gene36866:CDS |
| GTCGATGGTGAGTGGAGTTC+CGG | 0.410987 | 8.4:-64144395 | MS.gene36866:CDS |
| TAACAGGAATTCCAGCTGTT+TGG | 0.411065 | 8.4:-64144327 | MS.gene36866:CDS |
| TCATGTAGCGGAGTTGTAAC+AGG | 0.421560 | 8.4:-64144343 | MS.gene36866:CDS |
| CACATACCCTCCAAGAAAAT+CGG | 0.422233 | 8.4:+64144543 | None:intergenic |
| AGTTCCGGTAACCGGGGTTC+CGG | 0.425765 | 8.4:-64144380 | MS.gene36866:CDS |
| GTCACCGGAACCCCGGTTAC+CGG | 0.431972 | 8.4:+64144376 | None:intergenic |
| TCCGCTACATGAGCTATTAC+CGG | 0.458069 | 8.4:+64144354 | None:intergenic |
| TGTAGGGTAACTTCCGCCTT+CGG | 0.459071 | 8.4:+64144684 | None:intergenic |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8.4:-64144489 | MS.gene36866:CDS |
| TGGTAGTTTAACCAAACAGC+TGG | 0.466544 | 8.4:+64144316 | None:intergenic |
| CCTCCAAGAAAATCGGAAAC+AGG | 0.468174 | 8.4:+64144550 | None:intergenic |
| CCAACTTCTCGTTCCATGTA+GGG | 0.474471 | 8.4:+64144668 | None:intergenic |
| GCCTTCGGAATCAAGTTTCG+TGG | 0.489360 | 8.4:+64144699 | None:intergenic |
| CTCGAGTTCCATGTCGTCGA+TGG | 0.506800 | 8.4:-64144410 | MS.gene36866:CDS |
| GGTAGTAGTAGCAATAGTGT+TGG | 0.506913 | 8.4:-64144586 | MS.gene36866:CDS |
| GTAACCGGGGTTCCGGTGAC+CGG | 0.511262 | 8.4:-64144373 | MS.gene36866:CDS |
| TGAGTGGAGTTCCGGTAACC+GGG | 0.516054 | 8.4:-64144387 | MS.gene36866:CDS |
| GGTTGTAAATATTTCAGTAA+AGG | 0.518683 | 8.4:-64144458 | MS.gene36866:CDS |
| ATGGAACGAGAAGTTGGTAA+TGG | 0.536894 | 8.4:-64144662 | MS.gene36866:CDS |
| AAGGCGGAAGTTACCCTACA+TGG | 0.551053 | 8.4:-64144681 | MS.gene36866:CDS |
| TAGTAGCAATAGTGTTGGAA+TGG | 0.552031 | 8.4:-64144581 | MS.gene36866:CDS |
| TCCACTCACCATCGACGACA+TGG | 0.556286 | 8.4:+64144402 | None:intergenic |
| GTGATGAACCTTGCATGTAA+TGG | 0.558756 | 8.4:+64144634 | None:intergenic |
| CCCTACATGGAACGAGAAGT+TGG | 0.565961 | 8.4:-64144668 | MS.gene36866:CDS |
| ACCAACTTCTCGTTCCATGT+AGG | 0.582262 | 8.4:+64144667 | None:intergenic |
| TCCATGTCGTCGATGGTGAG+TGG | 0.586254 | 8.4:-64144403 | MS.gene36866:CDS |
| CATGAGCTATTACCGGTCAC+CGG | 0.597980 | 8.4:+64144361 | None:intergenic |
| GATGTGAAGTACAAAACAAG+AGG | 0.601532 | 8.4:-64144607 | MS.gene36866:CDS |
| TTCGTCACGGTTCAATGTGA+CGG | 0.611637 | 8.4:-64144742 | MS.gene36866:CDS |
| TGGATGTGCCATTACATGCA+AGG | 0.624126 | 8.4:-64144642 | MS.gene36866:CDS |
| ACGAAACTTGATTCCGAAGG+CGG | 0.631770 | 8.4:-64144697 | MS.gene36866:CDS |
| TAATAGAGTTAGGAGAAATG+GGG | 0.635926 | 8.4:-64144479 | MS.gene36866:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.642396 | 8.4:-64144700 | MS.gene36866:CDS |
| ATTACCGGTCACCGGAACCC+CGG | 0.657610 | 8.4:+64144369 | None:intergenic |
| ACCGGTAATAGCTCATGTAG+CGG | 0.660755 | 8.4:-64144355 | MS.gene36866:CDS |
| GAGTGGAGTTCCGGTAACCG+GGG | 0.677190 | 8.4:-64144386 | MS.gene36866:CDS |
| AAAAGGGAACACATTCGTCA+CGG | 0.677311 | 8.4:-64144755 | MS.gene36866:CDS |
| TCAATGTGACGGAAGCAATG+AGG | 0.684521 | 8.4:-64144731 | MS.gene36866:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | - | chr8.4:64144613-64144632 | MS.gene36866:CDS | 15.0% |
| !! | AGAATAAAAAAGCCATAAAA+GGG | - | chr8.4:64144352-64144371 | MS.gene36866:CDS | 20.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | - | chr8.4:64144619-64144638 | MS.gene36866:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | - | chr8.4:64144620-64144639 | MS.gene36866:CDS | 20.0% |
| ! | ATAATAGAGTTAGGAGAAAT+GGG | - | chr8.4:64144643-64144662 | MS.gene36866:CDS | 25.0% |
| ! | GAGAATAAAAAAGCCATAAA+AGG | - | chr8.4:64144351-64144370 | MS.gene36866:CDS | 25.0% |
| ! | GGTTGTAAATATTTCAGTAA+AGG | - | chr8.4:64144665-64144684 | MS.gene36866:CDS | 25.0% |
| GATAATAGAGTTAGGAGAAA+TGG | - | chr8.4:64144642-64144661 | MS.gene36866:CDS | 30.0% | |
| TAATAGAGTTAGGAGAAATG+GGG | - | chr8.4:64144644-64144663 | MS.gene36866:CDS | 30.0% | |
| GATGTGAAGTACAAAACAAG+AGG | - | chr8.4:64144516-64144535 | MS.gene36866:CDS | 35.0% | |
| ! | TAGTAGCAATAGTGTTGGAA+TGG | - | chr8.4:64144542-64144561 | MS.gene36866:CDS | 35.0% |
| AAAAGGGAACACATTCGTCA+CGG | - | chr8.4:64144368-64144387 | MS.gene36866:CDS | 40.0% | |
| CACATACCCTCCAAGAAAAT+CGG | + | chr8.4:64144583-64144602 | None:intergenic | 40.0% | |
| GGTAGTAGTAGCAATAGTGT+TGG | - | chr8.4:64144537-64144556 | MS.gene36866:CDS | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | - | chr8.4:64144634-64144653 | MS.gene36866:CDS | 40.0% | |
| GTGATGAACCTTGCATGTAA+TGG | + | chr8.4:64144492-64144511 | None:intergenic | 40.0% | |
| TAACAGGAATTCCAGCTGTT+TGG | - | chr8.4:64144796-64144815 | MS.gene36866:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+TGG | + | chr8.4:64144810-64144829 | None:intergenic | 40.0% | |
| ! | CTGTTTCCGATTTTCTTGGA+GGG | - | chr8.4:64144574-64144593 | MS.gene36866:CDS | 40.0% |
| ! | GACGAATGTGTTCCCTTTTA+TGG | + | chr8.4:64144367-64144386 | None:intergenic | 40.0% |
| ! | GTACCTGTTTCCGATTTTCT+TGG | - | chr8.4:64144570-64144589 | MS.gene36866:CDS | 40.0% |
| !! | ATGGAACGAGAAGTTGGTAA+TGG | - | chr8.4:64144461-64144480 | MS.gene36866:CDS | 40.0% |
| ACCAACTTCTCGTTCCATGT+AGG | + | chr8.4:64144459-64144478 | None:intergenic | 45.0% | |
| ACCACGAAACTTGATTCCGA+AGG | - | chr8.4:64144423-64144442 | MS.gene36866:CDS | 45.0% | |
| ACCGGTAATAGCTCATGTAG+CGG | - | chr8.4:64144768-64144787 | MS.gene36866:CDS | 45.0% | |
| ACGAAACTTGATTCCGAAGG+CGG | - | chr8.4:64144426-64144445 | MS.gene36866:CDS | 45.0% | |
| CCAACTTCTCGTTCCATGTA+GGG | + | chr8.4:64144458-64144477 | None:intergenic | 45.0% | |
| CCTCCAAGAAAATCGGAAAC+AGG | + | chr8.4:64144576-64144595 | None:intergenic | 45.0% | |
| TCCGCTACATGAGCTATTAC+CGG | + | chr8.4:64144772-64144791 | None:intergenic | 45.0% | |
| TTCGTCACGGTTCAATGTGA+CGG | - | chr8.4:64144381-64144400 | MS.gene36866:CDS | 45.0% | |
| ! | CCTGTTTCCGATTTTCTTGG+AGG | - | chr8.4:64144573-64144592 | MS.gene36866:CDS | 45.0% |
| ! | TCAATGTGACGGAAGCAATG+AGG | - | chr8.4:64144392-64144411 | MS.gene36866:CDS | 45.0% |
| ! | TCATGTAGCGGAGTTGTAAC+AGG | - | chr8.4:64144780-64144799 | MS.gene36866:CDS | 45.0% |
| ! | TGGATGTGCCATTACATGCA+AGG | - | chr8.4:64144481-64144500 | MS.gene36866:CDS | 45.0% |
| AAGGCGGAAGTTACCCTACA+TGG | - | chr8.4:64144442-64144461 | MS.gene36866:CDS | 50.0% | |
| CATGAGCTATTACCGGTCAC+CGG | + | chr8.4:64144765-64144784 | None:intergenic | 50.0% | |
| CCCTACATGGAACGAGAAGT+TGG | - | chr8.4:64144455-64144474 | MS.gene36866:CDS | 50.0% | |
| GCCTTCGGAATCAAGTTTCG+TGG | + | chr8.4:64144427-64144446 | None:intergenic | 50.0% | |
| TGTAGGGTAACTTCCGCCTT+CGG | + | chr8.4:64144442-64144461 | None:intergenic | 50.0% | |
| CTCGAGTTCCATGTCGTCGA+TGG | - | chr8.4:64144713-64144732 | MS.gene36866:CDS | 55.0% | |
| GTCGATGGTGAGTGGAGTTC+CGG | - | chr8.4:64144728-64144747 | MS.gene36866:CDS | 55.0% | |
| GTGAGTGGAGTTCCGGTAAC+CGG | - | chr8.4:64144735-64144754 | MS.gene36866:CDS | 55.0% | |
| TCCACTCACCATCGACGACA+TGG | + | chr8.4:64144724-64144743 | None:intergenic | 55.0% | |
| TCCATGTCGTCGATGGTGAG+TGG | - | chr8.4:64144720-64144739 | MS.gene36866:CDS | 55.0% | |
| TGAGTGGAGTTCCGGTAACC+GGG | - | chr8.4:64144736-64144755 | MS.gene36866:CDS | 55.0% | |
| AGTTCCGGTAACCGGGGTTC+CGG | - | chr8.4:64144743-64144762 | MS.gene36866:CDS | 60.0% | |
| ATTACCGGTCACCGGAACCC+CGG | + | chr8.4:64144757-64144776 | None:intergenic | 60.0% | |
| GAGTGGAGTTCCGGTAACCG+GGG | - | chr8.4:64144737-64144756 | MS.gene36866:CDS | 60.0% | |
| GTAACCGGGGTTCCGGTGAC+CGG | - | chr8.4:64144750-64144769 | MS.gene36866:CDS | 65.0% | |
| GTCACCGGAACCCCGGTTAC+CGG | + | chr8.4:64144750-64144769 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 64144303 | 64144842 | 64144303 | ID=MS.gene36866 |
| chr8.4 | mRNA | 64144303 | 64144842 | 64144303 | ID=MS.gene36866.t1;Parent=MS.gene36866 |
| chr8.4 | exon | 64144303 | 64144842 | 64144303 | ID=MS.gene36866.t1.exon1;Parent=MS.gene36866.t1 |
| chr8.4 | CDS | 64144303 | 64144842 | 64144303 | ID=cds.MS.gene36866.t1;Parent=MS.gene36866.t1 |
| Gene Sequence |
| Protein sequence |
