Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38123.t1 | XP_003618062.1 | 75 | 236 | 32 | 2 | 1 | 234 | 1 | 211 | 7.90E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38123.t1 | G7K0N6 | 75.0 | 236 | 32 | 2 | 1 | 234 | 1 | 211 | 5.7e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049409 | MS.gene38123 | 0.833452 | 5.29E-56 | -1.69E-46 |
| MS.gene049567 | MS.gene38123 | 0.822566 | 2.21E-53 | -1.69E-46 |
| MS.gene051492 | MS.gene38123 | 0.8205 | 6.64E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene38123 | 0.812884 | 3.39E-51 | -1.69E-46 |
| MS.gene051832 | MS.gene38123 | 0.807384 | 5.19E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene38123 | 0.805889 | 1.07E-49 | -1.69E-46 |
| MS.gene053094 | MS.gene38123 | 0.842089 | 3.20E-58 | -1.69E-46 |
| MS.gene054576 | MS.gene38123 | 0.814858 | 1.24E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene38123 | 0.825234 | 5.24E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene38123 | 0.823958 | 1.05E-53 | -1.69E-46 |
| MS.gene058477 | MS.gene38123 | 0.806451 | 8.17E-50 | -1.69E-46 |
| MS.gene05861 | MS.gene38123 | 0.809273 | 2.05E-50 | -1.69E-46 |
| MS.gene05969 | MS.gene38123 | 0.804431 | 2.17E-49 | -1.69E-46 |
| MS.gene059774 | MS.gene38123 | 0.856337 | 3.45E-62 | -1.69E-46 |
| MS.gene060412 | MS.gene38123 | 0.812009 | 5.26E-51 | -1.69E-46 |
| MS.gene060697 | MS.gene38123 | 0.85581 | 4.91E-62 | -1.69E-46 |
| MS.gene061047 | MS.gene38123 | 0.855716 | 5.24E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38123.t1 | MTR_5g098580 | 74.153 | 236 | 34 | 3 | 1 | 234 | 1 | 211 | 1.11e-101 | 294 |
| MS.gene38123.t1 | MTR_5g098610 | 77.928 | 222 | 34 | 4 | 1 | 216 | 1 | 213 | 2.63e-84 | 250 |
| MS.gene38123.t1 | MTR_5g098630 | 43.231 | 229 | 77 | 9 | 13 | 235 | 10 | 191 | 5.22e-34 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGAGGGAAATGCTCTTA+TGG | 0.351628 | 5.1:-79235047 | MS.gene38123:CDS |
| AACTTATGGTGATAACGTTA+TGG | 0.422749 | 5.1:-79235155 | MS.gene38123:CDS |
| GAGATTACAGAACCAACTTA+TGG | 0.427552 | 5.1:-79235169 | MS.gene38123:CDS |
| AGTGAAGAGTTTCGTGATAG+TGG | 0.437310 | 5.1:-79234993 | MS.gene38123:CDS |
| CTGATATGTAAAATTCGAAC+CGG | 0.464643 | 5.1:+79235197 | None:intergenic |
| TTGCATAAGGAATGGCTTCA+AGG | 0.464946 | 5.1:+79235369 | None:intergenic |
| CAAGAAGGATATTGCAGAGA+AGG | 0.490364 | 5.1:+79235311 | None:intergenic |
| TGATGAGTGGTTGATGAAGA+GGG | 0.490655 | 5.1:+79235256 | None:intergenic |
| AGAAGATGAAGGAAAAGAAG+AGG | 0.503671 | 5.1:-79235062 | MS.gene38123:CDS |
| GAAGATGAAGGAAAAGAAGA+GGG | 0.505978 | 5.1:-79235061 | MS.gene38123:CDS |
| ATAACGTTATCACCATAAGT+TGG | 0.521599 | 5.1:+79235157 | None:intergenic |
| TGTAGCTAAAGAATTGCATA+AGG | 0.524182 | 5.1:+79235356 | None:intergenic |
| TCGACAGCGACAAGAGTAAC+CGG | 0.524574 | 5.1:-79235216 | MS.gene38123:CDS |
| ATTGAAGCAGAAGAAGATGA+AGG | 0.541164 | 5.1:-79235073 | MS.gene38123:CDS |
| CTAAAGAATTGCATAAGGAA+TGG | 0.544450 | 5.1:+79235361 | None:intergenic |
| TTGATGAGTGGTTGATGAAG+AGG | 0.572497 | 5.1:+79235255 | None:intergenic |
| TTCAAGGACTGAGAGAGTAA+AGG | 0.580402 | 5.1:+79235385 | None:intergenic |
| ATAATGCAATAAAAGCAAGA+AGG | 0.623530 | 5.1:+79235296 | None:intergenic |
| GGTTACTCTTGTCGCTGTCG+AGG | 0.630249 | 5.1:+79235218 | None:intergenic |
| GATAGTGAAACCAGAAAATG+TGG | 0.638900 | 5.1:-79235098 | MS.gene38123:CDS |
| GAAACTAAACAAGAAATGTG+AGG | 0.665023 | 5.1:-79234885 | MS.gene38123:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATCAAAAAAATGAAAGC+TGG | - | chr5.1:79235393-79235412 | MS.gene38123:CDS | 20.0% |
| !!! | TTCTTCATTTTCTTCAATTT+GGG | + | chr5.1:79235241-79235260 | None:intergenic | 20.0% |
| ! | ATAATGCAATAAAAGCAAGA+AGG | + | chr5.1:79234960-79234979 | None:intergenic | 25.0% |
| !! | CAAACTATTTTCTTCTTCTT+GGG | + | chr5.1:79235133-79235152 | None:intergenic | 25.0% |
| !! | CTTCTTCATTTTCTTCAATT+TGG | + | chr5.1:79235242-79235261 | None:intergenic | 25.0% |
| !! | TCAAACTATTTTCTTCTTCT+TGG | + | chr5.1:79235134-79235153 | None:intergenic | 25.0% |
| ATAACGTTATCACCATAAGT+TGG | + | chr5.1:79235099-79235118 | None:intergenic | 30.0% | |
| CTAAAGAATTGCATAAGGAA+TGG | + | chr5.1:79234895-79234914 | None:intergenic | 30.0% | |
| CTGATATGTAAAATTCGAAC+CGG | + | chr5.1:79235059-79235078 | None:intergenic | 30.0% | |
| GAAACTAAACAAGAAATGTG+AGG | - | chr5.1:79235368-79235387 | MS.gene38123:CDS | 30.0% | |
| TGTAGCTAAAGAATTGCATA+AGG | + | chr5.1:79234900-79234919 | None:intergenic | 30.0% | |
| ! | AACTTATGGTGATAACGTTA+TGG | - | chr5.1:79235098-79235117 | MS.gene38123:CDS | 30.0% |
| ! | AGAAGAAGAAGTACAAAAAG+AGG | - | chr5.1:79235308-79235327 | MS.gene38123:CDS | 30.0% |
| ! | GAAGAAGAAGTACAAAAAGA+GGG | - | chr5.1:79235309-79235328 | MS.gene38123:CDS | 30.0% |
| !!! | ATGTTCAATGTTTTGATGAG+TGG | + | chr5.1:79235013-79235032 | None:intergenic | 30.0% |
| AGAAGATGAAGGAAAAGAAG+AGG | - | chr5.1:79235191-79235210 | MS.gene38123:CDS | 35.0% | |
| ATTGAAGCAGAAGAAGATGA+AGG | - | chr5.1:79235180-79235199 | MS.gene38123:CDS | 35.0% | |
| GAAGATGAAGGAAAAGAAGA+GGG | - | chr5.1:79235192-79235211 | MS.gene38123:CDS | 35.0% | |
| GAGATTACAGAACCAACTTA+TGG | - | chr5.1:79235084-79235103 | MS.gene38123:CDS | 35.0% | |
| GATAGTGAAACCAGAAAATG+TGG | - | chr5.1:79235155-79235174 | MS.gene38123:CDS | 35.0% | |
| ! | GCTTCAATAACCACATTTTC+TGG | + | chr5.1:79235168-79235187 | None:intergenic | 35.0% |
| AGAAGAGGGAAATGCTCTTA+TGG | - | chr5.1:79235206-79235225 | MS.gene38123:CDS | 40.0% | |
| AGTGAAGAGTTTCGTGATAG+TGG | - | chr5.1:79235260-79235279 | MS.gene38123:CDS | 40.0% | |
| CAAGAAGGATATTGCAGAGA+AGG | + | chr5.1:79234945-79234964 | None:intergenic | 40.0% | |
| TTCAAGGACTGAGAGAGTAA+AGG | + | chr5.1:79234871-79234890 | None:intergenic | 40.0% | |
| TTGCATAAGGAATGGCTTCA+AGG | + | chr5.1:79234887-79234906 | None:intergenic | 40.0% | |
| !! | TGATGAGTGGTTGATGAAGA+GGG | + | chr5.1:79235000-79235019 | None:intergenic | 40.0% |
| !! | TTGATGAGTGGTTGATGAAG+AGG | + | chr5.1:79235001-79235020 | None:intergenic | 40.0% |
| TCGACAGCGACAAGAGTAAC+CGG | - | chr5.1:79235037-79235056 | MS.gene38123:CDS | 50.0% | |
| GGTTACTCTTGTCGCTGTCG+AGG | + | chr5.1:79235038-79235057 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 79234784 | 79235491 | 79234784 | ID=MS.gene38123 |
| chr5.1 | mRNA | 79234784 | 79235491 | 79234784 | ID=MS.gene38123.t1;Parent=MS.gene38123 |
| chr5.1 | exon | 79234784 | 79235491 | 79234784 | ID=MS.gene38123.t1.exon1;Parent=MS.gene38123.t1 |
| chr5.1 | CDS | 79234784 | 79235491 | 79234784 | ID=cds.MS.gene38123.t1;Parent=MS.gene38123.t1 |
| Gene Sequence |
| Protein sequence |
