Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40607.t1 | XP_003610819.2 | 95 | 1129 | 14 | 2 | 1 | 1095 | 58 | 1178 | 0.00E+00 | 2046.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40607.t1 | Q40983 | 86.3 | 1130 | 112 | 3 | 1 | 1095 | 56 | 1177 | 0.0e+00 | 1907.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40607.t1 | G7K558 | 95.0 | 1129 | 14 | 2 | 1 | 1095 | 58 | 1178 | 0.0e+00 | 2046.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene40607 | 0.840643 | 7.68E-58 | -1.69E-46 |
| MS.gene050670 | MS.gene40607 | 0.809124 | 2.21E-50 | -1.69E-46 |
| MS.gene050704 | MS.gene40607 | 0.808527 | 2.96E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene40607 | 0.820406 | 6.97E-53 | -1.69E-46 |
| MS.gene051387 | MS.gene40607 | 0.822765 | 1.99E-53 | -1.69E-46 |
| MS.gene051521 | MS.gene40607 | 0.822874 | 1.88E-53 | -1.69E-46 |
| MS.gene051525 | MS.gene40607 | 0.828449 | 8.93E-55 | -1.69E-46 |
| MS.gene051588 | MS.gene40607 | 0.80537 | 1.38E-49 | -1.69E-46 |
| MS.gene051941 | MS.gene40607 | 0.819228 | 1.30E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene40607 | 0.812039 | 5.18E-51 | -1.69E-46 |
| MS.gene052346 | MS.gene40607 | 0.810133 | 1.34E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene40607 | 0.83793 | 3.89E-57 | -1.69E-46 |
| MS.gene053169 | MS.gene40607 | 0.837511 | 4.98E-57 | -1.69E-46 |
| MS.gene054556 | MS.gene40607 | 0.821244 | 4.48E-53 | -1.69E-46 |
| MS.gene054719 | MS.gene40607 | 0.82057 | 6.40E-53 | -1.69E-46 |
| MS.gene055369 | MS.gene40607 | 0.814622 | 1.40E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene40607 | 0.897803 | 9.87E-77 | -1.69E-46 |
| MS.gene05553 | MS.gene40607 | 0.808677 | 2.75E-50 | -1.69E-46 |
| MS.gene05556 | MS.gene40607 | 0.806905 | 6.55E-50 | -1.69E-46 |
| MS.gene055741 | MS.gene40607 | 0.876329 | 1.52E-68 | -1.69E-46 |
| MS.gene055744 | MS.gene40607 | 0.875918 | 2.11E-68 | -1.69E-46 |
| MS.gene055909 | MS.gene40607 | 0.842745 | 2.14E-58 | -1.69E-46 |
| MS.gene055911 | MS.gene40607 | 0.851798 | 7.02E-61 | -1.69E-46 |
| MS.gene05601 | MS.gene40607 | 0.809573 | 1.77E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene40607 | 0.810253 | 1.26E-50 | -1.69E-46 |
| MS.gene056514 | MS.gene40607 | 0.835202 | 1.92E-56 | -1.69E-46 |
| MS.gene05664 | MS.gene40607 | 0.801308 | 9.58E-49 | -1.69E-46 |
| MS.gene05666 | MS.gene40607 | 0.802419 | 5.66E-49 | -1.69E-46 |
| MS.gene057019 | MS.gene40607 | 0.838169 | 3.37E-57 | -1.69E-46 |
| MS.gene057396 | MS.gene40607 | 0.80655 | 7.79E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene40607 | 0.811844 | 5.71E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene40607 | 0.831924 | 1.27E-55 | -1.69E-46 |
| MS.gene057823 | MS.gene40607 | 0.850774 | 1.37E-60 | -1.69E-46 |
| MS.gene057825 | MS.gene40607 | 0.818462 | 1.94E-52 | -1.69E-46 |
| MS.gene058007 | MS.gene40607 | 0.819182 | 1.33E-52 | -1.69E-46 |
| MS.gene058090 | MS.gene40607 | 0.826273 | 2.97E-54 | -1.69E-46 |
| MS.gene058120 | MS.gene40607 | 0.886677 | 2.78E-72 | -1.69E-46 |
| MS.gene058444 | MS.gene40607 | 0.819146 | 1.35E-52 | -1.69E-46 |
| MS.gene058700 | MS.gene40607 | 0.877248 | 7.32E-69 | -1.69E-46 |
| MS.gene058948 | MS.gene40607 | 0.876062 | 1.88E-68 | -1.69E-46 |
| MS.gene058949 | MS.gene40607 | 0.834263 | 3.32E-56 | -1.69E-46 |
| MS.gene059368 | MS.gene40607 | 0.800598 | 1.34E-48 | -1.69E-46 |
| MS.gene059420 | MS.gene40607 | 0.822368 | 2.46E-53 | -1.69E-46 |
| MS.gene059455 | MS.gene40607 | 0.827378 | 1.62E-54 | -1.69E-46 |
| MS.gene059674 | MS.gene40607 | 0.832533 | 8.96E-56 | -1.69E-46 |
| MS.gene059868 | MS.gene40607 | 0.811614 | 6.41E-51 | -1.69E-46 |
| MS.gene060372 | MS.gene40607 | 0.856848 | 2.44E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 251 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCTGGTCCATAACTTTA+TGG | 0.132951 | 5.3:+2229469 | None:intergenic |
| TTAGAATTTATAGCTGATTT+TGG | 0.161929 | 5.3:-2228807 | MS.gene40607:CDS |
| TCAATCTGGTTAACAGTCTT+TGG | 0.188437 | 5.3:+2232148 | None:intergenic |
| AGCTTTGTAGCATCTGTTTC+AGG | 0.198729 | 5.3:+2228228 | None:intergenic |
| CTAGTTGGTGGAACTTTATT+TGG | 0.204443 | 5.3:+2234356 | None:intergenic |
| TTTCTGAAATTATAAATTCT+AGG | 0.244955 | 5.3:-2225308 | MS.gene40607:intron |
| TTGAATTAAACTTGTTTGAT+AGG | 0.257126 | 5.3:-2224982 | MS.gene40607:CDS |
| GTTCGCAGACTTAAAGAATT+TGG | 0.278808 | 5.3:-2229621 | MS.gene40607:CDS |
| TGGAATTCCTGTCAACTATA+AGG | 0.281973 | 5.3:-2228163 | MS.gene40607:intron |
| ACCTTTCACTTCAGTTGAAT+TGG | 0.282488 | 5.3:-2230107 | MS.gene40607:CDS |
| GATGATGGTACAAGTTCTTT+TGG | 0.293705 | 5.3:+2228303 | None:intergenic |
| CTTGCGGCGTGTGGCTCATC+TGG | 0.300514 | 5.3:+2234533 | None:intergenic |
| GAATTGGATCATAGTGATTC+TGG | 0.303764 | 5.3:-2230091 | MS.gene40607:CDS |
| GCATCTGTTTCAGGACTTAC+TGG | 0.305082 | 5.3:+2228237 | None:intergenic |
| TTCAAATTTGCACTGCTTCC+AGG | 0.314465 | 5.3:+2231825 | None:intergenic |
| TGATCTCCTTCCATCTGTTC+TGG | 0.315346 | 5.3:-2232773 | MS.gene40607:CDS |
| CAGAATTACGGACCGGGAAT+TGG | 0.320342 | 5.3:-2224776 | MS.gene40607:CDS |
| TCAATCAAAAGTATTTAATA+AGG | 0.342883 | 5.3:-2231892 | MS.gene40607:CDS |
| CAATCTGGTTAACAGTCTTT+GGG | 0.346497 | 5.3:+2232149 | None:intergenic |
| TGGGCCTGCACCAAACCGTT+GGG | 0.347169 | 5.3:-2225592 | MS.gene40607:CDS |
| TATTTCTATCTCACCTTCCT+TGG | 0.349556 | 5.3:+2224309 | None:intergenic |
| TACGGAGGAGTTCATATCTA+TGG | 0.350048 | 5.3:-2226750 | MS.gene40607:CDS |
| GAACTCCTCCGTAGATTCTA+AGG | 0.352257 | 5.3:+2226760 | None:intergenic |
| TTTAACTCTGCTATCTGTTA+AGG | 0.355370 | 5.3:-2226366 | MS.gene40607:CDS |
| AGGGCGTGTGCTTATATTGC+TGG | 0.356943 | 5.3:-2225612 | MS.gene40607:CDS |
| AAATAAATAGTACCTCGTTC+AGG | 0.357379 | 5.3:+2232746 | None:intergenic |
| ATTCTTTGGTTCTGCAGTTA+TGG | 0.357818 | 5.3:+2230039 | None:intergenic |
| TTCCATTTGCAAGGCGGCGC+CGG | 0.360128 | 5.3:+2228181 | None:intergenic |
| GAAAGTTTACTTGCTGTTGC+TGG | 0.362354 | 5.3:-2229438 | MS.gene40607:CDS |
| ATCTGTTCTTCTAATCCAAT+TGG | 0.362403 | 5.3:+2232268 | None:intergenic |
| AACTTTCATGCCCTTGTCTC+TGG | 0.364641 | 5.3:+2229454 | None:intergenic |
| AGAGTTATTCATGGCGGATT+AGG | 0.365568 | 5.3:-2235653 | MS.gene40607:CDS |
| GTAGCCACTCCAAGTGCATT+TGG | 0.367057 | 5.3:-2231996 | MS.gene40607:CDS |
| GTTCTTCAGGTATCCATTGT+AGG | 0.367240 | 5.3:-2226239 | MS.gene40607:intron |
| CACTAACCCTGTCCAATTCC+CGG | 0.374454 | 5.3:+2224764 | None:intergenic |
| AGTCTTGCCAGATTTGTTCC+TGG | 0.375851 | 5.3:-2235506 | MS.gene40607:CDS |
| TGTATCTTCTGTTGATAACT+TGG | 0.381510 | 5.3:-2229520 | MS.gene40607:CDS |
| CTCGACTGCTCACAAACTTA+TGG | 0.381657 | 5.3:-2226447 | MS.gene40607:CDS |
| TTTAAAGTTTCTTGTGGCCT+GGG | 0.385858 | 5.3:+2226163 | None:intergenic |
| GACGGAATAAACCAGATGTT+TGG | 0.388098 | 5.3:-2235625 | MS.gene40607:CDS |
| CAGCTTCATGTCTCATAAGA+AGG | 0.389704 | 5.3:+2224391 | None:intergenic |
| CCTAAGCTGTCTGTTGGTCT+AGG | 0.390603 | 5.3:-2231951 | MS.gene40607:CDS |
| GAATAGTGTTTCCAAACATC+TGG | 0.390897 | 5.3:+2235614 | None:intergenic |
| AGCAAGAGGCACAACTTTGC+TGG | 0.394383 | 5.3:+2235558 | None:intergenic |
| ACACATAATTGGTCACTTCC+TGG | 0.395057 | 5.3:-2231843 | MS.gene40607:CDS |
| GTCGAGCGTTCCAAGCTCTT+GGG | 0.396483 | 5.3:+2226464 | None:intergenic |
| AAGCTACATGATGAGGAAAC+TGG | 0.396918 | 5.3:-2228210 | MS.gene40607:CDS |
| AGTGAAAGAAGAGTTATTCA+TGG | 0.398063 | 5.3:-2235662 | MS.gene40607:CDS |
| GTCCTCCTGTGACACATAAT+TGG | 0.404153 | 5.3:-2231854 | MS.gene40607:CDS |
| ATCCTAGATGAAAGGAACTT+TGG | 0.410743 | 5.3:+2232510 | None:intergenic |
| ACCCGGCGCCGCCTTGCAAA+TGG | 0.410811 | 5.3:-2228183 | MS.gene40607:CDS |
| CATTATCAATAGTCTCTAAC+AGG | 0.416233 | 5.3:+2225547 | None:intergenic |
| ATATTTGACATACTTCAATC+TGG | 0.418048 | 5.3:+2232134 | None:intergenic |
| ACTCGAACGACGTTTCTTTG+TGG | 0.418295 | 5.3:+2235526 | None:intergenic |
| GAATGATTTCCGCAGATAAT+GGG | 0.419683 | 5.3:-2225419 | MS.gene40607:intron |
| ATGTACAAAGTAGCATTTGC+AGG | 0.420127 | 5.3:+2232193 | None:intergenic |
| AAAAGCTCCAGGAACAAATC+TGG | 0.423512 | 5.3:+2235499 | None:intergenic |
| TGCTGGAACGGTGCCGCCGC+CGG | 0.425729 | 5.3:+2235575 | None:intergenic |
| GCATGCAAGAATGTTCTTAG+AGG | 0.427394 | 5.3:-2224811 | MS.gene40607:CDS |
| CACACGCCGCAAGCACAGCT+TGG | 0.427484 | 5.3:-2234523 | MS.gene40607:CDS |
| ACCTCAGGTTCTGGTTCTAT+AGG | 0.427533 | 5.3:+2228648 | None:intergenic |
| ATTACAGATGCTATCAAAGC+TGG | 0.428025 | 5.3:-2228681 | MS.gene40607:CDS |
| CTGAGCAAAAGGTTTCCAAT+TGG | 0.428095 | 5.3:-2232283 | MS.gene40607:CDS |
| AAGTCAAATGCATACTGGTT+GGG | 0.428487 | 5.3:-2224363 | MS.gene40607:CDS |
| AAGTTTGTTTGATTCTGAAC+TGG | 0.428739 | 5.3:-2234471 | MS.gene40607:CDS |
| CAAACTTATGGTAGCAATGT+TGG | 0.429496 | 5.3:-2226435 | MS.gene40607:CDS |
| ATCCAAAGTTCCTTTCATCT+AGG | 0.443590 | 5.3:-2232512 | MS.gene40607:CDS |
| AGTCGAGCGTTCCAAGCTCT+TGG | 0.444884 | 5.3:+2226463 | None:intergenic |
| AGTTGACCTCTATGCAACTT+GGG | 0.447384 | 5.3:+2234407 | None:intergenic |
| TCCATTTGCAAGGCGGCGCC+GGG | 0.448024 | 5.3:+2228182 | None:intergenic |
| TAAGAGGTCCACACCTTGCT+TGG | 0.449108 | 5.3:+2224920 | None:intergenic |
| CAAAGAAAAGAGGATGATTA+CGG | 0.453585 | 5.3:+2225349 | None:intergenic |
| CATAGAGGTCAACTAAAGAA+TGG | 0.455806 | 5.3:-2234398 | MS.gene40607:CDS |
| AACTCTTGTAGGTTTGATGA+TGG | 0.456850 | 5.3:+2228288 | None:intergenic |
| CAAGCTGTGCTTGCGGCGTG+TGG | 0.457895 | 5.3:+2234524 | None:intergenic |
| CTGAAAACAAGCTGAGCAAA+AGG | 0.459642 | 5.3:-2232294 | MS.gene40607:CDS |
| GATCTGTGATTGTGGGTGTT+AGG | 0.459657 | 5.3:-2227669 | MS.gene40607:CDS |
| TTCAGGACTTACTGGAATGA+AGG | 0.460656 | 5.3:+2228245 | None:intergenic |
| TAACTGCAGAACCAAAGAAT+TGG | 0.462242 | 5.3:-2230036 | MS.gene40607:CDS |
| TAAAGAATTTGGTGTTACTC+AGG | 0.463511 | 5.3:-2229610 | MS.gene40607:CDS |
| GTGGTGCAGGTTAATTCTGT+TGG | 0.467175 | 5.3:-2228840 | MS.gene40607:intron |
| CTAGTTCCTAAGCTGTCTGT+TGG | 0.468225 | 5.3:-2231957 | MS.gene40607:CDS |
| TAGTTGACCTCTATGCAACT+TGG | 0.469429 | 5.3:+2234406 | None:intergenic |
| ATTTATTGAGCTCACCTAGT+TGG | 0.470410 | 5.3:+2234341 | None:intergenic |
| CTGGGCCTGCACCAAACCGT+TGG | 0.472097 | 5.3:-2225593 | MS.gene40607:CDS |
| CACTTCTTGAAACTGCAAAC+CGG | 0.474006 | 5.3:+2226097 | None:intergenic |
| GCAGTAAAACCCCAACGGTT+TGG | 0.475100 | 5.3:+2225582 | None:intergenic |
| AAATTAAGTCAAATGCATAC+TGG | 0.476920 | 5.3:-2224368 | MS.gene40607:CDS |
| TTACGGACCGGGAATTGGAC+AGG | 0.478639 | 5.3:-2224771 | MS.gene40607:CDS |
| TCATTTCGATCATCTTCATC+CGG | 0.479008 | 5.3:-2226116 | MS.gene40607:CDS |
| GGCGTGATGCGGTTGATTGT+TGG | 0.479383 | 5.3:-2227727 | MS.gene40607:CDS |
| GCGTTCCAAGCTCTTGGGGA+TGG | 0.481044 | 5.3:+2226469 | None:intergenic |
| AATAAATAGTACCTCGTTCA+GGG | 0.483291 | 5.3:+2232747 | None:intergenic |
| TCTGCATAGCAACAGAATTA+CGG | 0.487986 | 5.3:-2224788 | MS.gene40607:CDS |
| TTGTTTGATAGGCTTAAACT+AGG | 0.489015 | 5.3:-2224971 | MS.gene40607:CDS |
| TTCTTCGCTGCTTTCTCCAC+CGG | 0.491989 | 5.3:-2235594 | MS.gene40607:CDS |
| GGACTTACTGGAATGAAGGT+TGG | 0.492207 | 5.3:+2228249 | None:intergenic |
| TGCTCTGAGCCATAAAGTTA+TGG | 0.493905 | 5.3:-2229478 | MS.gene40607:CDS |
| TATTGAGCTCACCTAGTTGG+TGG | 0.495075 | 5.3:+2234344 | None:intergenic |
| CCGATCCATCCCCAAGAGCT+TGG | 0.495120 | 5.3:-2226474 | MS.gene40607:CDS |
| GCAAGCACAGCTTGGCCCGA+TGG | 0.496641 | 5.3:-2234515 | MS.gene40607:CDS |
| TAAGTCAAATGCATACTGGT+TGG | 0.496647 | 5.3:-2224364 | MS.gene40607:CDS |
| TTGTGGCCTGGGCTGTGCCA+AGG | 0.497094 | 5.3:+2226174 | None:intergenic |
| AGAAGATACATTATCAATCA+TGG | 0.497351 | 5.3:+2229533 | None:intergenic |
| TTCAATTAATATGTTCTGCA+AGG | 0.497787 | 5.3:-2231763 | MS.gene40607:intron |
| AGCCTTTCAATTGCTTCACA+TGG | 0.500883 | 5.3:-2226690 | MS.gene40607:CDS |
| CAATTGCTCCTTAGAATCTA+CGG | 0.502181 | 5.3:-2226768 | MS.gene40607:CDS |
| AGCAACAATTGCAGCAGGAA+GGG | 0.503734 | 5.3:+2228770 | None:intergenic |
| CTATGCAACTTGGGGTGAGA+TGG | 0.506548 | 5.3:+2234416 | None:intergenic |
| GCTGTAATGAGTCAGTTTGT+TGG | 0.511934 | 5.3:-2226341 | MS.gene40607:CDS |
| TCCAAGTGCATTTGGTGCAA+TGG | 0.515628 | 5.3:-2231988 | MS.gene40607:CDS |
| ATTGCTTCACATGGTGCTTG+AGG | 0.517776 | 5.3:-2226681 | MS.gene40607:intron |
| AAATGCTACTTTGTACATTG+TGG | 0.518304 | 5.3:-2232188 | MS.gene40607:CDS |
| AATGCTACTTTGTACATTGT+GGG | 0.519686 | 5.3:-2232187 | MS.gene40607:CDS |
| CTTGCTTGGAGTTGATGTTA+CGG | 0.521244 | 5.3:+2224934 | None:intergenic |
| AGCTTCATGTCTCATAAGAA+GGG | 0.521533 | 5.3:+2224392 | None:intergenic |
| AAAGTTTACTTGCTGTTGCT+GGG | 0.522236 | 5.3:-2229437 | MS.gene40607:CDS |
| CAACAAACCTTATAGTTGAC+AGG | 0.522507 | 5.3:+2228156 | None:intergenic |
| CTTAAATGATACTGATGAGA+GGG | 0.524205 | 5.3:-2225631 | MS.gene40607:CDS |
| TGTTTGATAGGCTTAAACTA+GGG | 0.527227 | 5.3:-2224970 | MS.gene40607:CDS |
| TAAGAAAATTTCACGAGCGT+TGG | 0.527913 | 5.3:-2232222 | MS.gene40607:CDS |
| AACTCGTGTTGAAATATTTG+TGG | 0.529098 | 5.3:+2231798 | None:intergenic |
| CATAGTGATTCTGGAAGAGA+AGG | 0.529485 | 5.3:-2230082 | MS.gene40607:CDS |
| AAACCCCAACGGTTTGGTGC+AGG | 0.530874 | 5.3:+2225588 | None:intergenic |
| GATGAAGATGATCGAAATGA+AGG | 0.530990 | 5.3:+2226119 | None:intergenic |
| GCTGAGAGTTATGATTCAAA+AGG | 0.531442 | 5.3:-2227691 | MS.gene40607:CDS |
| ATAGAGGTCAACTAAAGAAT+GGG | 0.531653 | 5.3:-2234397 | MS.gene40607:CDS |
| GATAATGCATCATCAGTCAA+TGG | 0.531854 | 5.3:-2225531 | MS.gene40607:intron |
| CTCAGTGAAGTCACCTACAA+TGG | 0.532677 | 5.3:+2226226 | None:intergenic |
| GGGCGTGTGCTTATATTGCT+GGG | 0.533864 | 5.3:-2225611 | MS.gene40607:CDS |
| ATTGTTATACCAAAGAAAAG+AGG | 0.535218 | 5.3:+2225339 | None:intergenic |
| GAGGCACAACTTTGCTGGAA+CGG | 0.536343 | 5.3:+2235563 | None:intergenic |
| TGACAGGAATTCCATTTGCA+AGG | 0.538200 | 5.3:+2228172 | None:intergenic |
| TGAAAGGAACTTTGGATGGA+AGG | 0.538444 | 5.3:+2232518 | None:intergenic |
| ATTCTAAGGAGCAATTGATC+AGG | 0.540832 | 5.3:+2226774 | None:intergenic |
| GGTTAATTCTGTTGGTGCTG+AGG | 0.541527 | 5.3:-2228832 | MS.gene40607:CDS |
| AGCATCTTCTGTTCCAAGGA+AGG | 0.542868 | 5.3:-2224322 | MS.gene40607:CDS |
| TACAAGCATCTTCTGTTCCA+AGG | 0.544828 | 5.3:-2224326 | MS.gene40607:CDS |
| CAAATAAAGTTCCACCAACT+AGG | 0.545112 | 5.3:-2234355 | MS.gene40607:intron |
| GTCGTGTTGGCAACTTCTCA+AGG | 0.545248 | 5.3:-2227630 | MS.gene40607:CDS |
| GCTGCTTTCTCCACCGGCGG+CGG | 0.545487 | 5.3:-2235588 | MS.gene40607:CDS |
| TAGATGAAAGGAACTTTGGA+TGG | 0.545911 | 5.3:+2232514 | None:intergenic |
| AAAGAATTTGGTGTTACTCA+GGG | 0.546941 | 5.3:-2229609 | MS.gene40607:CDS |
| TTATATGCATATGCAGCTTG+AGG | 0.547221 | 5.3:-2228328 | MS.gene40607:CDS |
| ATGCGGTTGATTGTTGGTGG+TGG | 0.547818 | 5.3:-2227721 | MS.gene40607:CDS |
| TGTTTGATTCTGAACTGGAA+AGG | 0.548532 | 5.3:-2234466 | MS.gene40607:CDS |
| TTCAGGGCATCCAGAACAGA+TGG | 0.549882 | 5.3:+2232763 | None:intergenic |
| AGTGTTCGCTATCTTTCAGA+AGG | 0.550950 | 5.3:+2229562 | None:intergenic |
| CCTAGACCAACAGACAGCTT+AGG | 0.554811 | 5.3:+2231951 | None:intergenic |
| GATGAAGAGGATGATGAGCA+AGG | 0.556827 | 5.3:-2233663 | MS.gene40607:CDS |
| AAGCAACAATTGCAGCAGGA+AGG | 0.556996 | 5.3:+2228769 | None:intergenic |
| AGTGACCAATTATGTGTCAC+AGG | 0.557165 | 5.3:+2231849 | None:intergenic |
| TGGCAACTTCTCAAGGGAGC+AGG | 0.557815 | 5.3:-2227623 | MS.gene40607:intron |
| CGTTATCCCGTAGAGTAAAA+CGG | 0.557836 | 5.3:+2226723 | None:intergenic |
| AAAGTTCATATCGAAGGAGT+TGG | 0.557965 | 5.3:-2228735 | MS.gene40607:CDS |
| AGAGTATCGAGTTGATTGCC+AGG | 0.558108 | 5.3:-2232436 | MS.gene40607:intron |
| GCCATTGCACCAAATGCACT+TGG | 0.558481 | 5.3:+2231987 | None:intergenic |
| AAGCTGTCTGTTGGTCTAGG+TGG | 0.558676 | 5.3:-2231948 | MS.gene40607:CDS |
| GAAGATGATCGAAATGAAGG+TGG | 0.558715 | 5.3:+2226122 | None:intergenic |
| AGATGCTATCAAAGCTGGGT+TGG | 0.561457 | 5.3:-2228676 | MS.gene40607:CDS |
| TCGAGCGTTCCAAGCTCTTG+GGG | 0.561613 | 5.3:+2226465 | None:intergenic |
| ATCGGGCCAAGCTGTGCTTG+CGG | 0.562924 | 5.3:+2234517 | None:intergenic |
| CCTTGGTAGTGGTAGGAGCA+TGG | 0.564021 | 5.3:+2233523 | None:intergenic |
| ATGATGTGCAGCATAGTGTC+TGG | 0.565568 | 5.3:-2226544 | MS.gene40607:intron |
| GCAACAATTGCAGCAGGAAG+GGG | 0.565568 | 5.3:+2228771 | None:intergenic |
| CACCATGTGAAGCAATTGAA+AGG | 0.567117 | 5.3:+2226688 | None:intergenic |
| TCTGAACTGGAAAGGCTAGA+GGG | 0.569040 | 5.3:-2234458 | MS.gene40607:CDS |
| ATGCTACTTTGTACATTGTG+GGG | 0.570695 | 5.3:-2232186 | MS.gene40607:CDS |
| GCAGCAGGAAGGGGTGCGGT+AGG | 0.570918 | 5.3:+2228780 | None:intergenic |
| TTAATTAAAATACCTCGTCA+AGG | 0.570990 | 5.3:+2229406 | None:intergenic |
| TGGAACGGTGCCGCCGCCGG+TGG | 0.573638 | 5.3:+2235578 | None:intergenic |
| CAAGTTCCACATGCAACTGT+TGG | 0.574898 | 5.3:-2234557 | MS.gene40607:CDS |
| AAGATGATCGAAATGAAGGT+GGG | 0.575983 | 5.3:+2226123 | None:intergenic |
| TTCAAAAGGATCTGTGATTG+TGG | 0.578143 | 5.3:-2227677 | MS.gene40607:CDS |
| CTTAGTGAGGGAGGTCGTGT+TGG | 0.578924 | 5.3:-2227643 | MS.gene40607:CDS |
| CGTAACATCAACTCCAAGCA+AGG | 0.579372 | 5.3:-2224933 | MS.gene40607:intron |
| ATCTGTATAAGCATTTGAGC+GGG | 0.579721 | 5.3:+2233569 | None:intergenic |
| ACACAAGCAACAATTGCAGC+AGG | 0.579788 | 5.3:+2228765 | None:intergenic |
| AGACTACCTTGGCACAGCCC+AGG | 0.579817 | 5.3:-2226180 | MS.gene40607:CDS |
| TGCTACAAAGCTACATGATG+AGG | 0.580067 | 5.3:-2228217 | MS.gene40607:CDS |
| CCAAGCTCTTGGGGATGGAT+CGG | 0.580093 | 5.3:+2226474 | None:intergenic |
| TCAGTTTGTTGGAGATAACA+TGG | 0.581115 | 5.3:-2226330 | MS.gene40607:CDS |
| TGCTGGATCGATAGATGAAG+AGG | 0.581607 | 5.3:-2233676 | MS.gene40607:CDS |
| TGAAAGATCAACAAATACAA+TGG | 0.581989 | 5.3:-2231916 | MS.gene40607:CDS |
| ACAACAGTCAGAGATTCACT+AGG | 0.582922 | 5.3:-2225025 | MS.gene40607:CDS |
| GTTGACCTCTATGCAACTTG+GGG | 0.585763 | 5.3:+2234408 | None:intergenic |
| GCACACATGGAAGTTCATGC+TGG | 0.587346 | 5.3:-2233693 | MS.gene40607:CDS |
| AATGGTCCAAACCTTGGTAG+TGG | 0.587681 | 5.3:+2233512 | None:intergenic |
| TTACAGATGCTATCAAAGCT+GGG | 0.590155 | 5.3:-2228680 | MS.gene40607:CDS |
| TATTAGAGTTGCTGTTCATG+AGG | 0.590651 | 5.3:-2230005 | MS.gene40607:intron |
| ACTGGTGTAGACAATGAGAA+AGG | 0.595304 | 5.3:-2232023 | MS.gene40607:CDS |
| CTTATGGTAGCAATGTTGGA+TGG | 0.595666 | 5.3:-2226431 | MS.gene40607:CDS |
| TTCTGAACTGGAAAGGCTAG+AGG | 0.596519 | 5.3:-2234459 | MS.gene40607:CDS |
| GTTAAATTCTCTAGTGAACT+CGG | 0.596523 | 5.3:+2226383 | None:intergenic |
| TTGATAGGCTTAAACTAGGG+TGG | 0.596819 | 5.3:-2224967 | MS.gene40607:CDS |
| TCGTGTTGAAATATTTGTGG+TGG | 0.597128 | 5.3:+2231801 | None:intergenic |
| GGGTGTTAGGACACTTAGTG+AGG | 0.597948 | 5.3:-2227656 | MS.gene40607:CDS |
| CATGGATGTGAAACACTGTA+TGG | 0.598973 | 5.3:+2233541 | None:intergenic |
| TTCTGCAGTTATGGTAAGCG+TGG | 0.599096 | 5.3:+2230048 | None:intergenic |
| GGGCCTGCACCAAACCGTTG+GGG | 0.601511 | 5.3:-2225591 | MS.gene40607:CDS |
| TAATTAAAATACCTCGTCAA+GGG | 0.601557 | 5.3:+2229407 | None:intergenic |
| GTGATGCGGTTGATTGTTGG+TGG | 0.602048 | 5.3:-2227724 | MS.gene40607:CDS |
| CTGAACTGGAAAGGCTAGAG+GGG | 0.602586 | 5.3:-2234457 | MS.gene40607:CDS |
| TCCAATTCAACTGAAGTGAA+AGG | 0.603293 | 5.3:+2230106 | None:intergenic |
| TCTAAGGATAAGCTTCGTCA+TGG | 0.603518 | 5.3:-2235452 | MS.gene40607:CDS |
| TGTGGAAGCAAGACAGCAAG+AGG | 0.605809 | 5.3:+2235544 | None:intergenic |
| TGCACCAAATGCACTTGGAG+TGG | 0.606182 | 5.3:+2231992 | None:intergenic |
| TGCTACTTTGTACATTGTGG+GGG | 0.606858 | 5.3:-2232185 | MS.gene40607:CDS |
| TCATGTATTCTAGACTACCT+TGG | 0.607465 | 5.3:-2226191 | MS.gene40607:CDS |
| TTCGCTGCTTTCTCCACCGG+CGG | 0.607765 | 5.3:-2235591 | MS.gene40607:CDS |
| AAAGTTATGGACCAGAGACA+AGG | 0.613208 | 5.3:-2229465 | MS.gene40607:CDS |
| AATTCAACTGAAGTGAAAGG+TGG | 0.613252 | 5.3:+2230109 | None:intergenic |
| AATTGCAGCAGGAAGGGGTG+CGG | 0.613415 | 5.3:+2228776 | None:intergenic |
| TTCTCTAGTGAACTCGGTGT+AGG | 0.615673 | 5.3:+2226389 | None:intergenic |
| TGTACAAAGTAGCATTTGCA+GGG | 0.617045 | 5.3:+2232194 | None:intergenic |
| CAGGAATTCCATTTGCAAGG+CGG | 0.620446 | 5.3:+2228175 | None:intergenic |
| ATAGCAACAGAATTACGGAC+CGG | 0.622001 | 5.3:-2224783 | MS.gene40607:CDS |
| CAACAGTCAGAGATTCACTA+GGG | 0.622688 | 5.3:-2225024 | MS.gene40607:CDS |
| TCTTAAATGATACTGATGAG+AGG | 0.630526 | 5.3:-2225632 | MS.gene40607:CDS |
| TCTGATGCTGTACCAACAGA+AGG | 0.632220 | 5.3:-2225390 | MS.gene40607:CDS |
| CATCAGCAGTAAAACCCCAA+CGG | 0.634961 | 5.3:+2225577 | None:intergenic |
| GATGCTGTACCAACAGAAGG+TGG | 0.635426 | 5.3:-2225387 | MS.gene40607:CDS |
| ATGAGGAAACTGGAATCACC+CGG | 0.635803 | 5.3:-2228200 | MS.gene40607:CDS |
| GGGCATCCAGAACAGATGGA+AGG | 0.639515 | 5.3:+2232767 | None:intergenic |
| TCTCACCCCAAGTTGCATAG+AGG | 0.644862 | 5.3:-2234413 | MS.gene40607:CDS |
| TCTAAAAGTGAAACTCAAAG+TGG | 0.644914 | 5.3:-2227748 | MS.gene40607:CDS |
| TCAAAAGGATCTGTGATTGT+GGG | 0.645874 | 5.3:-2227676 | MS.gene40607:CDS |
| TCGTGTTGGCAACTTCTCAA+GGG | 0.646722 | 5.3:-2227629 | MS.gene40607:CDS |
| GTGTTCGCTATCTTTCAGAA+GGG | 0.649126 | 5.3:+2229563 | None:intergenic |
| TACGGACCGGGAATTGGACA+GGG | 0.649583 | 5.3:-2224770 | MS.gene40607:intron |
| AAGTTATGGACCAGAGACAA+GGG | 0.651760 | 5.3:-2229464 | MS.gene40607:CDS |
| TCCTATAGAACCAGAACCTG+AGG | 0.651863 | 5.3:-2228649 | MS.gene40607:intron |
| AATCTGTATAAGCATTTGAG+CGG | 0.651909 | 5.3:+2233568 | None:intergenic |
| ATGGCGGATTAGGTTTACGA+CGG | 0.662827 | 5.3:-2235643 | MS.gene40607:CDS |
| TCTGGACCAACAGTTGCATG+TGG | 0.667090 | 5.3:+2234551 | None:intergenic |
| GGTGTTAGGACACTTAGTGA+GGG | 0.668232 | 5.3:-2227655 | MS.gene40607:CDS |
| TAGCAACAGAATTACGGACC+GGG | 0.668758 | 5.3:-2224782 | MS.gene40607:CDS |
| GTTAGGACACTTAGTGAGGG+AGG | 0.672451 | 5.3:-2227652 | MS.gene40607:CDS |
| TGTTCTGGATGCCCTGAACG+AGG | 0.675029 | 5.3:-2232758 | MS.gene40607:intron |
| CCATGCTCCTACCACTACCA+AGG | 0.675098 | 5.3:-2233523 | MS.gene40607:intron |
| GTCCAAACCTTGGTAGTGGT+AGG | 0.675270 | 5.3:+2233516 | None:intergenic |
| GGATGTGAAACACTGTATGG+TGG | 0.682829 | 5.3:+2233544 | None:intergenic |
| AAACTCAAAGTGGCGTGATG+CGG | 0.685110 | 5.3:-2227738 | MS.gene40607:CDS |
| AGAAAAGAGGATGATTACGG+AGG | 0.685165 | 5.3:+2225352 | None:intergenic |
| TGTAGGTGACTTCACTGAGG+AGG | 0.686813 | 5.3:-2226222 | MS.gene40607:CDS |
| TGGGACAGTTACCCTTGACG+AGG | 0.705381 | 5.3:-2229418 | MS.gene40607:intron |
| GACCAATTATGTGTCACAGG+AGG | 0.706544 | 5.3:+2231852 | None:intergenic |
| GAAAGAAGAGTTATTCATGG+CGG | 0.736930 | 5.3:-2235659 | MS.gene40607:CDS |
| TTGCTCCTTAGAATCTACGG+AGG | 0.759582 | 5.3:-2226765 | MS.gene40607:CDS |
| GTTTGTTGGAGATAACATGG+AGG | 0.773308 | 5.3:-2226327 | MS.gene40607:intron |
| CATTGTAGGTGACTTCACTG+AGG | 0.783685 | 5.3:-2226225 | MS.gene40607:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 2224314 | 2235682 | 2224314 | ID=MS.gene40607 |
| chr5.3 | mRNA | 2224314 | 2235682 | 2224314 | ID=MS.gene40607.t1;Parent=MS.gene40607 |
| chr5.3 | exon | 2235448 | 2235682 | 2235448 | ID=MS.gene40607.t1.exon1;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2235448 | 2235682 | 2235448 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2234356 | 2234591 | 2234356 | ID=MS.gene40607.t1.exon2;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2234356 | 2234591 | 2234356 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2233524 | 2233722 | 2233524 | ID=MS.gene40607.t1.exon3;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2233524 | 2233722 | 2233524 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2232759 | 2232803 | 2232759 | ID=MS.gene40607.t1.exon4;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2232759 | 2232803 | 2232759 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2232437 | 2232544 | 2232437 | ID=MS.gene40607.t1.exon5;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2232437 | 2232544 | 2232437 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2232147 | 2232335 | 2232147 | ID=MS.gene40607.t1.exon6;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2232147 | 2232335 | 2232147 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2231764 | 2232060 | 2231764 | ID=MS.gene40607.t1.exon7;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2231764 | 2232060 | 2231764 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2230006 | 2230140 | 2230006 | ID=MS.gene40607.t1.exon8;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2230006 | 2230140 | 2230006 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2229419 | 2229643 | 2229419 | ID=MS.gene40607.t1.exon9;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2229419 | 2229643 | 2229419 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2228650 | 2228853 | 2228650 | ID=MS.gene40607.t1.exon10;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2228650 | 2228853 | 2228650 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2228164 | 2228358 | 2228164 | ID=MS.gene40607.t1.exon11;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2228164 | 2228358 | 2228164 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2227624 | 2227773 | 2227624 | ID=MS.gene40607.t1.exon12;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2227624 | 2227773 | 2227624 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2226682 | 2226819 | 2226682 | ID=MS.gene40607.t1.exon13;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2226682 | 2226819 | 2226682 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2226328 | 2226555 | 2226328 | ID=MS.gene40607.t1.exon14;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2226328 | 2226555 | 2226328 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2226100 | 2226252 | 2226100 | ID=MS.gene40607.t1.exon15;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2226100 | 2226252 | 2226100 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2225532 | 2225658 | 2225532 | ID=MS.gene40607.t1.exon16;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2225532 | 2225658 | 2225532 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2225309 | 2225426 | 2225309 | ID=MS.gene40607.t1.exon17;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2225309 | 2225426 | 2225309 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2224934 | 2225054 | 2224934 | ID=MS.gene40607.t1.exon18;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2224934 | 2225054 | 2224934 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2224771 | 2224851 | 2224771 | ID=MS.gene40607.t1.exon19;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2224771 | 2224851 | 2224771 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| chr5.3 | exon | 2224314 | 2224424 | 2224314 | ID=MS.gene40607.t1.exon20;Parent=MS.gene40607.t1 |
| chr5.3 | CDS | 2224314 | 2224424 | 2224314 | ID=cds.MS.gene40607.t1;Parent=MS.gene40607.t1 |
| Gene Sequence |
| Protein sequence |
