Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41733.t1 | XP_003624630.2 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 6.00E-18 | 99.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41733.t1 | Q9LVC0 | 54.1 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 7.0e-07 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41733.t1 | G7KZH1 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 4.4e-18 | 99.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene41733 | 0.804734 | 1.87E-49 | -1.69E-46 |
| MS.gene04915 | MS.gene41733 | 0.80946 | 1.87E-50 | -1.69E-46 |
| MS.gene04917 | MS.gene41733 | 0.812009 | 5.26E-51 | -1.69E-46 |
| MS.gene049887 | MS.gene41733 | 0.800203 | 1.61E-48 | -1.69E-46 |
| MS.gene049983 | MS.gene41733 | 0.824812 | 6.59E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene41733 | 0.826733 | 2.31E-54 | -1.69E-46 |
| MS.gene050759 | MS.gene41733 | 0.807482 | 4.95E-50 | -1.69E-46 |
| MS.gene050941 | MS.gene41733 | 0.83824 | 3.23E-57 | -1.69E-46 |
| MS.gene051003 | MS.gene41733 | 0.806924 | 6.50E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene41733 | 0.801985 | 6.95E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene41733 | 0.817061 | 4.01E-52 | -1.69E-46 |
| MS.gene052342 | MS.gene41733 | 0.82086 | 5.49E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene41733 | 0.814271 | 1.68E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene41733 | 0.81695 | 4.25E-52 | -1.69E-46 |
| MS.gene053456 | MS.gene41733 | 0.818012 | 2.45E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene41733 | 0.810905 | 9.14E-51 | -1.69E-46 |
| MS.gene056480 | MS.gene41733 | 0.837219 | 5.91E-57 | -1.69E-46 |
| MS.gene059283 | MS.gene41733 | 0.808624 | 2.83E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene41733 | 0.809083 | 2.25E-50 | -1.69E-46 |
| MS.gene060296 | MS.gene41733 | 0.807411 | 5.12E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene41733 | 0.862089 | 6.48E-64 | -1.69E-46 |
| MS.gene060932 | MS.gene41733 | 0.862032 | 6.75E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41733.t1 | MTR_7g085780 | 98.305 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 2.48e-33 | 108 |
| MS.gene41733.t1 | MTR_6g029260 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 8.81e-15 | 61.6 |
| MS.gene41733.t1 | MTR_6g029330 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 1.94e-13 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41733.t1 | AT5G56540 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 2.43e-14 | 60.5 |
| MS.gene41733.t1 | AT3G13520 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 4.02e-11 | 52.4 |
Find 7 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCATAGCACTTGCATT+TGG | 0.360344 | 7.2:-22092889 | MS.gene41733:CDS |
| GGCAGTTATGGTTGCTATGT+TGG | 0.451707 | 7.2:-22093004 | MS.gene41733:CDS |
| GTAGCATCAGAAGCTGGACT+TGG | 0.472931 | 7.2:+22092940 | None:intergenic |
| TCAGAAGCTGGACTTGGAGC+TGG | 0.503706 | 7.2:+22092946 | None:intergenic |
| AGTGTTGTAGCATCAGAAGC+TGG | 0.542379 | 7.2:+22092934 | None:intergenic |
| ATGAGAGAAGCAAAAGCAGT+TGG | 0.550611 | 7.2:+22092904 | None:intergenic |
| ATGTTGGTGATAGCAACAAG+TGG | 0.753532 | 7.2:-22092988 | MS.gene41733:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAGATGAACATTTTTG+TGG | - | chr7.2:22092884-22092903 | MS.gene41733:CDS | 25.0% |
| ! | GAACATTTTTGTGGCAGTTA+TGG | - | chr7.2:22092893-22092912 | MS.gene41733:CDS | 35.0% |
| ATGAGAGAAGCAAAAGCAGT+TGG | + | chr7.2:22093008-22093027 | None:intergenic | 40.0% | |
| TCTCTCATAGCACTTGCATT+TGG | - | chr7.2:22093020-22093039 | MS.gene41733:CDS | 40.0% | |
| !! | ATGTTGGTGATAGCAACAAG+TGG | - | chr7.2:22092921-22092940 | MS.gene41733:CDS | 40.0% |
| AGTGTTGTAGCATCAGAAGC+TGG | + | chr7.2:22092978-22092997 | None:intergenic | 45.0% | |
| GGCAGTTATGGTTGCTATGT+TGG | - | chr7.2:22092905-22092924 | MS.gene41733:CDS | 45.0% | |
| GTAGCATCAGAAGCTGGACT+TGG | + | chr7.2:22092972-22092991 | None:intergenic | 50.0% | |
| TCAGAAGCTGGACTTGGAGC+TGG | + | chr7.2:22092966-22092985 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 22092876 | 22093055 | 22092876 | ID=MS.gene41733 |
| chr7.2 | mRNA | 22092876 | 22093055 | 22092876 | ID=MS.gene41733.t1;Parent=MS.gene41733 |
| chr7.2 | exon | 22092876 | 22093055 | 22092876 | ID=MS.gene41733.t1.exon1;Parent=MS.gene41733.t1 |
| chr7.2 | CDS | 22092876 | 22093055 | 22092876 | ID=cds.MS.gene41733.t1;Parent=MS.gene41733.t1 |
| Gene Sequence |
| Protein sequence |
