Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42625.t1 | RHN70436.1 | 85.4 | 96 | 12 | 1 | 1 | 94 | 1 | 96 | 1.10E-37 | 165.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42625.t1 | A0A396IXK8 | 85.4 | 96 | 12 | 1 | 1 | 94 | 1 | 96 | 7.9e-38 | 165.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050317 | MS.gene42625 | 0.802204 | 6.27E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene42625 | 0.828666 | 7.92E-55 | -1.69E-46 |
| MS.gene050955 | MS.gene42625 | 0.818188 | 2.23E-52 | -1.69E-46 |
| MS.gene051233 | MS.gene42625 | 0.825436 | 4.69E-54 | -1.69E-46 |
| MS.gene051756 | MS.gene42625 | 0.807308 | 5.39E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene42625 | 0.845372 | 4.23E-59 | -1.69E-46 |
| MS.gene052527 | MS.gene42625 | 0.803955 | 2.72E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene42625 | 0.805756 | 1.14E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene42625 | 0.828778 | 7.44E-55 | -1.69E-46 |
| MS.gene054699 | MS.gene42625 | 0.803655 | 3.14E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene42625 | 0.814103 | 1.83E-51 | -1.69E-46 |
| MS.gene056764 | MS.gene42625 | 0.809243 | 2.08E-50 | -1.69E-46 |
| MS.gene057057 | MS.gene42625 | 0.802202 | 6.27E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene42625 | 0.827759 | 1.31E-54 | -1.69E-46 |
| MS.gene057392 | MS.gene42625 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene42625 | 0.826023 | 3.41E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene42625 | 0.821058 | 4.94E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene42625 | 0.808758 | 2.65E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGTTGGATTTCTTGCTGAT+AGG | 0.335101 | 3.4:-91901837 | MS.gene42625:CDS |
| GCTATACTTGGTATAAAATA+CGG | 0.377678 | 3.4:-91901903 | MS.gene42625:CDS |
| CGTTGGATTTCTTGCTGATA+GGG | 0.388507 | 3.4:-91901836 | MS.gene42625:CDS |
| GAGGGTGTCATCTACCAATT+CGG | 0.402371 | 3.4:-91902134 | MS.gene42625:CDS |
| AGGGTGTCATCTACCAATTC+GGG | 0.431438 | 3.4:-91902133 | MS.gene42625:CDS |
| CTATACTTGGTATAAAATAC+GGG | 0.523048 | 3.4:-91901902 | MS.gene42625:CDS |
| TGTCCGACAAAAGCTATACT+TGG | 0.542972 | 3.4:-91901915 | MS.gene42625:CDS |
| GCTATAGTAGTCACGGGCGT+TGG | 0.571195 | 3.4:-91901853 | MS.gene42625:CDS |
| GTCGGTGCGACAGATATTGA+AGG | 0.600333 | 3.4:-91902944 | MS.gene42625:intron |
| AAACCACGCTATAGTAGTCA+CGG | 0.605546 | 3.4:-91901860 | MS.gene42625:CDS |
| AACCACGCTATAGTAGTCAC+GGG | 0.623391 | 3.4:-91901859 | MS.gene42625:CDS |
| CGCCCGTGACTACTATAGCG+TGG | 0.702785 | 3.4:+91901857 | None:intergenic |
| GAATTGGTAGATGACACCCT+CGG | 0.729335 | 3.4:+91902136 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTCTTTTTAATTGTTTAT+AGG | - | chr3.4:91902819-91902838 | MS.gene42625:intron | 10.0% |
| !! | AAAATAATAGATTCAAAAGA+TGG | + | chr3.4:91902420-91902439 | None:intergenic | 15.0% |
| !! | ATTTCAACAATAAACAAATT+AGG | + | chr3.4:91902225-91902244 | None:intergenic | 15.0% |
| !! | CTAGAAAATCTTATATTATT+TGG | - | chr3.4:91902348-91902367 | MS.gene42625:intron | 15.0% |
| !! | TTTCAACAATAAACAAATTA+GGG | + | chr3.4:91902224-91902243 | None:intergenic | 15.0% |
| !!! | ATAATAATTTTAGTTCTATG+TGG | - | chr3.4:91902791-91902810 | MS.gene42625:intron | 15.0% |
| !!! | TAATACCAACTATTTTATTT+TGG | - | chr3.4:91902593-91902612 | MS.gene42625:intron | 15.0% |
| !! | ACACAAATTTATGGAAAAAT+AGG | + | chr3.4:91902283-91902302 | None:intergenic | 20.0% |
| !! | ATGCAAAATATATATTGTAG+AGG | + | chr3.4:91902768-91902787 | None:intergenic | 20.0% |
| !! | CACAAATTTATGGAAAAATA+GGG | + | chr3.4:91902282-91902301 | None:intergenic | 20.0% |
| !! | GTAATCCAAAATAAAATAGT+TGG | + | chr3.4:91902601-91902620 | None:intergenic | 20.0% |
| !! | TGCAAAATATATATTGTAGA+GGG | + | chr3.4:91902767-91902786 | None:intergenic | 20.0% |
| !! | TTAATACAACGTAAGATAAT+TGG | + | chr3.4:91902576-91902595 | None:intergenic | 20.0% |
| !!! | TTTGATAATCTAAAAACCTT+TGG | - | chr3.4:91902519-91902538 | MS.gene42625:intron | 20.0% |
| ! | AAAATGTCTCAACTAATTGA+CGG | + | chr3.4:91902106-91902125 | None:intergenic | 25.0% |
| ! | AAATTCTTGCAAATTTCCAA+AGG | + | chr3.4:91902538-91902557 | None:intergenic | 25.0% |
| ! | ATAGAGCTTACACAAATTTA+TGG | + | chr3.4:91902292-91902311 | None:intergenic | 25.0% |
| ! | CTATACTTGGTATAAAATAC+GGG | - | chr3.4:91902860-91902879 | MS.gene42625:intron | 25.0% |
| ! | GCTATACTTGGTATAAAATA+CGG | - | chr3.4:91902859-91902878 | MS.gene42625:intron | 25.0% |
| !! | GAATCATTTTCTTAGATCTA+GGG | - | chr3.4:91901859-91901878 | MS.gene42625:CDS | 25.0% |
| !! | TGAATCATTTTCTTAGATCT+AGG | - | chr3.4:91901858-91901877 | MS.gene42625:CDS | 25.0% |
| !! | TTTTATCAAGCAACAGAAAA+TGG | - | chr3.4:91902669-91902688 | MS.gene42625:intron | 25.0% |
| !!! | CATGTTTGTATTTTGAGTTA+CGG | - | chr3.4:91902371-91902390 | MS.gene42625:intron | 25.0% |
| !!! | GGTTTTTAGATTATCAAAAC+AGG | + | chr3.4:91902517-91902536 | None:intergenic | 25.0% |
| CGGAGAATTAGGATATATAA+CGG | + | chr3.4:91902086-91902105 | None:intergenic | 30.0% | |
| ! | ATACCAAGTATAGCTTTTGT+CGG | + | chr3.4:91902853-91902872 | None:intergenic | 30.0% |
| !! | TGTGTTGTGATTTTAACACA+TGG | - | chr3.4:91902451-91902470 | MS.gene42625:intron | 30.0% |
| CAACTAATTGACGGAGAATT+AGG | + | chr3.4:91902097-91902116 | None:intergenic | 35.0% | |
| TGTAAAAAACATGCCCGAAT+TGG | + | chr3.4:91902645-91902664 | None:intergenic | 35.0% | |
| ! | ATTTTGGATTACAGATTCCG+AGG | - | chr3.4:91902609-91902628 | MS.gene42625:intron | 35.0% |
| ! | TCTTAGATCTAGGGTTTGTA+CGG | - | chr3.4:91901868-91901887 | MS.gene42625:CDS | 35.0% |
| ! | TTTTGGATTACAGATTCCGA+GGG | - | chr3.4:91902610-91902629 | MS.gene42625:intron | 35.0% |
| !! | GGATATATAACGGATCGAAA+CGG | + | chr3.4:91902076-91902095 | None:intergenic | 35.0% |
| !!! | ATTCGGGCATGTTTTTTACA+CGG | - | chr3.4:91902645-91902664 | MS.gene42625:intron | 35.0% |
| !!! | GTTTGTATTTTGAGTTACGG+TGG | - | chr3.4:91902374-91902393 | MS.gene42625:intron | 35.0% |
| AAACCACGCTATAGTAGTCA+CGG | - | chr3.4:91902902-91902921 | MS.gene42625:intron | 40.0% | |
| CGTTGGATTTCTTGCTGATA+GGG | - | chr3.4:91902926-91902945 | MS.gene42625:intron | 40.0% | |
| TGTCCGACAAAAGCTATACT+TGG | - | chr3.4:91902847-91902866 | MS.gene42625:intron | 40.0% | |
| AACCACGCTATAGTAGTCAC+GGG | - | chr3.4:91902903-91902922 | MS.gene42625:intron | 45.0% | |
| GAATTGGTAGATGACACCCT+CGG | + | chr3.4:91902629-91902648 | None:intergenic | 45.0% | |
| GCGTTGGATTTCTTGCTGAT+AGG | - | chr3.4:91902925-91902944 | MS.gene42625:intron | 45.0% | |
| ! | AGGGTGTCATCTACCAATTC+GGG | - | chr3.4:91902629-91902648 | MS.gene42625:intron | 45.0% |
| !! | GAGGGTGTCATCTACCAATT+CGG | - | chr3.4:91902628-91902647 | MS.gene42625:intron | 45.0% |
| !!! | ATTTTAACAATAAAAAAATT+AGG | + | chr3.4:91902135-91902154 | None:intergenic | 5.0% |
| !!! | TTTTAACAATAAAAAAATTA+GGG | + | chr3.4:91902134-91902153 | None:intergenic | 5.0% |
| GTCGGTGCGACAGATATTGA+AGG | - | chr3.4:91901818-91901837 | MS.gene42625:CDS | 50.0% | |
| GCTATAGTAGTCACGGGCGT+TGG | - | chr3.4:91902909-91902928 | MS.gene42625:intron | 55.0% | |
| CGCCCGTGACTACTATAGCG+TGG | + | chr3.4:91902908-91902927 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 91901813 | 91902971 | 91901813 | ID=MS.gene42625 |
| chr3.4 | mRNA | 91901813 | 91902971 | 91901813 | ID=MS.gene42625.t1;Parent=MS.gene42625 |
| chr3.4 | exon | 91902945 | 91902971 | 91902945 | ID=MS.gene42625.t1.exon1;Parent=MS.gene42625.t1 |
| chr3.4 | CDS | 91902945 | 91902971 | 91902945 | ID=cds.MS.gene42625.t1;Parent=MS.gene42625.t1 |
| chr3.4 | exon | 91902035 | 91902161 | 91902035 | ID=MS.gene42625.t1.exon2;Parent=MS.gene42625.t1 |
| chr3.4 | CDS | 91902035 | 91902161 | 91902035 | ID=cds.MS.gene42625.t1;Parent=MS.gene42625.t1 |
| chr3.4 | exon | 91901813 | 91901943 | 91901813 | ID=MS.gene42625.t1.exon3;Parent=MS.gene42625.t1 |
| chr3.4 | CDS | 91901813 | 91901943 | 91901813 | ID=cds.MS.gene42625.t1;Parent=MS.gene42625.t1 |
| Gene Sequence |
| Protein sequence |
