Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053045.t1 | XP_003604547.2 | 99 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 2.80E-47 | 197.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053045.t1 | A7HM43 | 54.2 | 83 | 38 | 0 | 2 | 84 | 4 | 86 | 9.6e-20 | 97.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053045.t1 | G7JKN8 | 99.0 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 2.1e-47 | 197.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049580 | MS.gene053045 | 0.812565 | 3.98E-51 | -1.69E-46 |
| MS.gene051699 | MS.gene053045 | 0.811916 | 5.51E-51 | -1.69E-46 |
| MS.gene051902 | MS.gene053045 | 0.804809 | 1.81E-49 | -1.69E-46 |
| MS.gene052108 | MS.gene053045 | 0.806763 | 7.02E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene053189 | 0.811161 | 8.04E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene053340 | 0.805047 | 1.61E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene054222 | 0.811735 | 6.03E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene055841 | 0.822845 | 1.90E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene056986 | 0.810323 | 1.22E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene057102 | 0.810899 | 9.16E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene057999 | 0.802034 | 6.79E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene05985 | -0.805326 | 1.41E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene062501 | 0.815603 | 8.50E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene062512 | 0.813763 | 2.17E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene063247 | 0.802788 | 4.75E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene06372 | 0.800187 | 1.62E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene065513 | 0.807316 | 5.36E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene068565 | 0.814814 | 1.27E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene069828 | 0.804836 | 1.78E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene07339 | 0.806833 | 6.79E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene073670 | 0.8367 | 8.02E-57 | -1.69E-46 |
| MS.gene053045 | MS.gene073867 | 0.821989 | 3.01E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene08108 | 0.8179 | 2.59E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene08582 | 0.806242 | 9.05E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene09850 | 0.806267 | 8.94E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene20064 | 0.826383 | 2.80E-54 | -1.69E-46 |
| MS.gene053045 | MS.gene22433 | 0.824065 | 9.88E-54 | -1.69E-46 |
| MS.gene053045 | MS.gene23060 | 0.823287 | 1.50E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene23695 | 0.80567 | 1.19E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene25686 | 0.800018 | 1.75E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene27228 | 0.8152 | 1.04E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene28980 | 0.808337 | 3.25E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene29492 | 0.814267 | 1.68E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene29794 | 0.801351 | 9.38E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene32551 | 0.822138 | 2.78E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene32552 | 0.832124 | 1.13E-55 | -1.69E-46 |
| MS.gene053045 | MS.gene32866 | 0.805223 | 1.48E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene35530 | 0.819725 | 9.99E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene36625 | 0.800371 | 1.49E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene38568 | 0.809852 | 1.54E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene39190 | 0.836323 | 1.00E-56 | -1.69E-46 |
| MS.gene053045 | MS.gene40608 | 0.803845 | 2.87E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene40908 | 0.815296 | 9.94E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene41086 | 0.801085 | 1.06E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene41866 | 0.806449 | 8.18E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene42302 | 0.822172 | 2.73E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene42625 | 0.805756 | 1.14E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene46390 | 0.814054 | 1.87E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene50013 | 0.804356 | 2.25E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene51145 | 0.822735 | 2.02E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene52420 | 0.80858 | 2.89E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene52808 | 0.807137 | 5.85E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene54090 | 0.808054 | 3.74E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene54331 | 0.806026 | 1.00E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene56178 | 0.814895 | 1.22E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene57128 | 0.800908 | 1.16E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene58284 | 0.801315 | 9.54E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene59505 | 0.812984 | 3.22E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene59540 | 0.822063 | 2.89E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene59616 | 0.815639 | 8.34E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene60384 | 0.816632 | 5.00E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene61254 | 0.809993 | 1.44E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene61257 | 0.817933 | 2.55E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene62676 | 0.810293 | 1.24E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene62677 | 0.810619 | 1.05E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene64395 | 0.805292 | 1.43E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene64674 | 0.812952 | 3.27E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene64806 | 0.812241 | 4.68E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene65294 | 0.808012 | 3.82E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene65295 | 0.804648 | 1.95E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene66476 | 0.80004 | 1.74E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene66960 | 0.816618 | 5.04E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene66979 | 0.844736 | 6.28E-59 | -1.69E-46 |
| MS.gene053045 | MS.gene67136 | 0.807303 | 5.40E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene67179 | 0.800664 | 1.30E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene67485 | 0.825606 | 4.28E-54 | -1.69E-46 |
| MS.gene053045 | MS.gene69820 | 0.801952 | 7.06E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene69821 | 0.817158 | 3.81E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene72126 | 0.8075 | 4.90E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene72376 | 0.800685 | 1.28E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene72620 | 0.800761 | 1.24E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene72748 | 0.80515 | 1.53E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene73012 | 0.801767 | 7.71E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene73234 | 0.817387 | 3.39E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene73781 | 0.809714 | 1.65E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene74275 | 0.819949 | 8.88E-53 | -1.69E-46 |
| MS.gene053045 | MS.gene74972 | 0.808435 | 3.10E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene75148 | 0.81759 | 3.05E-52 | -1.69E-46 |
| MS.gene053045 | MS.gene76248 | 0.803666 | 3.13E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene78773 | 0.801168 | 1.02E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene82841 | 0.940154 | 3.78E-100 | -1.69E-46 |
| MS.gene053045 | MS.gene82938 | 0.803307 | 3.71E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene83326 | 0.805826 | 1.11E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene85627 | 0.814518 | 1.48E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene87673 | 0.807806 | 4.22E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene87969 | 0.843467 | 1.38E-58 | -1.69E-46 |
| MS.gene053045 | MS.gene88351 | 0.80267 | 5.03E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene89605 | 0.802278 | 6.05E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene90861 | 0.807017 | 6.21E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene91203 | 0.800463 | 1.42E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene92135 | 0.804733 | 1.88E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene92775 | 0.81343 | 2.57E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene95469 | 0.808319 | 3.28E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene96121 | 0.813488 | 2.50E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene96156 | 0.813854 | 2.07E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene96209 | 0.805963 | 1.04E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene98141 | 0.801492 | 8.78E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene99848 | 0.816191 | 6.28E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053045.t1 | MTR_4g014360 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 2.78e-72 | 210 |
| MS.gene053045.t1 | MTR_6g005030 | 97.087 | 103 | 3 | 0 | 1 | 103 | 52 | 154 | 8.12e-70 | 205 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053045.t1 | AT3G18880 | 70.526 | 95 | 28 | 0 | 1 | 95 | 1 | 95 | 2.48e-47 | 147 |
| MS.gene053045.t1 | AT3G18880 | 70.526 | 95 | 28 | 0 | 1 | 95 | 1 | 95 | 2.48e-47 | 147 |
| MS.gene053045.t1 | AT1G49400 | 79.518 | 83 | 17 | 0 | 1 | 83 | 1 | 83 | 1.25e-46 | 145 |
| MS.gene053045.t1 | AT1G79850 | 39.744 | 78 | 47 | 0 | 1 | 78 | 50 | 127 | 4.21e-13 | 61.6 |
Find 20 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGCGCACTTCCAAGTTTA+TGG | 0.135840 | 6.2:+1038887 | MS.gene053045:CDS |
| AGGTTCGATTGGATTCTTCT+AGG | 0.331841 | 6.2:+1039228 | MS.gene053045:intron |
| TGGAAGTGCGCTTAATATAT+CGG | 0.362502 | 6.2:-1038878 | None:intergenic |
| CTTGTCATTTCAGGTTCGAT+TGG | 0.439317 | 6.2:+1039217 | MS.gene053045:intron |
| TTCGAATAAGATGCAGAAAT+CGG | 0.439794 | 6.2:+1038812 | MS.gene053045:CDS |
| GAGAAGATGAAGCAAGTAGT+AGG | 0.480871 | 6.2:+1038780 | None:intergenic |
| GATGAAGCAAGTAGTAGGAA+TGG | 0.481818 | 6.2:+1038785 | None:intergenic |
| TCTGAGCAAGCGTAAACATT+GGG | 0.508662 | 6.2:+1039253 | MS.gene053045:CDS |
| TTCATCATGAGCCATAAACT+TGG | 0.509537 | 6.2:-1038898 | None:intergenic |
| CAAATTTATGATGTAGATGT+AGG | 0.528358 | 6.2:-1039362 | None:intergenic |
| CTCTGAGCAAGCGTAAACAT+TGG | 0.545204 | 6.2:+1039252 | MS.gene053045:CDS |
| TAAGATGCAGAAATCGGTTG+TGG | 0.553827 | 6.2:+1038818 | MS.gene053045:CDS |
| TTCTCAGACACCGTTGCAGA+GGG | 0.577883 | 6.2:-1039317 | None:intergenic |
| GAAGCAAGTAGTAGGAATGG+TGG | 0.579237 | 6.2:+1038788 | MS.gene053045:CDS |
| ATATGTTCCTCCCTCTGCAA+CGG | 0.584593 | 6.2:+1039307 | MS.gene053045:CDS |
| AATGCGAGCTCGAACTCTGT+AGG | 0.604238 | 6.2:+1039338 | MS.gene053045:CDS |
| GAAAACAATCTTTGCAACAT+CGG | 0.608322 | 6.2:+1038918 | MS.gene053045:CDS |
| ATTCTCAGACACCGTTGCAG+AGG | 0.611069 | 6.2:-1039318 | None:intergenic |
| TCAGACACCGTTGCAGAGGG+AGG | 0.631588 | 6.2:-1039314 | None:intergenic |
| CAATGTTTACGCTTGCTCAG+AGG | 0.639617 | 6.2:-1039251 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTCACAATACTAACT+CGG | - | chr6.2:1038947-1038966 | None:intergenic | 20.0% |
| ! | TAACAACAATGGTAAAAGAT+AGG | - | chr6.2:1038972-1038991 | None:intergenic | 25.0% |
| GAAAACAATCTTTGCAACAT+CGG | + | chr6.2:1038918-1038937 | MS.gene053045:CDS | 30.0% | |
| TTAACAAAACCAAGAACACA+CGG | - | chr6.2:1039169-1039188 | None:intergenic | 30.0% | |
| TTCGAATAAGATGCAGAAAT+CGG | + | chr6.2:1038812-1038831 | MS.gene053045:CDS | 30.0% | |
| ! | AACAGTGTCTTGTACAATTT+TGG | + | chr6.2:1039138-1039157 | MS.gene053045:intron | 30.0% |
| ! | TTACCATTGTTGTTAAACAG+CGG | + | chr6.2:1038977-1038996 | MS.gene053045:intron | 30.0% |
| !! | TATATCGGTTGAACATTTTG+TGG | - | chr6.2:1038866-1038885 | None:intergenic | 30.0% |
| !!! | TTTGATCAAACTGCTGTTTT+TGG | + | chr6.2:1039107-1039126 | MS.gene053045:intron | 30.0% |
| AGGCAACAATTTGTGCTAAA+TGG | - | chr6.2:1039053-1039072 | None:intergenic | 35.0% | |
| TTCATCATGAGCCATAAACT+TGG | - | chr6.2:1038901-1038920 | None:intergenic | 35.0% | |
| ! | TGGAAGTGCGCTTAATATAT+CGG | - | chr6.2:1038881-1038900 | None:intergenic | 35.0% |
| AGGTTCGATTGGATTCTTCT+AGG | + | chr6.2:1039228-1039247 | MS.gene053045:intron | 40.0% | |
| TAAGATGCAGAAATCGGTTG+TGG | + | chr6.2:1038818-1038837 | MS.gene053045:CDS | 40.0% | |
| TAAGCGCACTTCCAAGTTTA+TGG | + | chr6.2:1038887-1038906 | MS.gene053045:CDS | 40.0% | |
| TCTGAGCAAGCGTAAACATT+GGG | + | chr6.2:1039253-1039272 | MS.gene053045:CDS | 40.0% | |
| ! | ATCGGTTGAACATTTTGTGG+TGG | - | chr6.2:1038863-1038882 | None:intergenic | 40.0% |
| !! | CTTGTCATTTCAGGTTCGAT+TGG | + | chr6.2:1039217-1039236 | MS.gene053045:intron | 40.0% |
| !! | GCTTTTGTGCTTGTCATTTC+AGG | + | chr6.2:1039208-1039227 | MS.gene053045:intron | 40.0% |
| ATATGTTCCTCCCTCTGCAA+CGG | + | chr6.2:1039307-1039326 | MS.gene053045:CDS | 45.0% | |
| CAATGTTTACGCTTGCTCAG+AGG | - | chr6.2:1039254-1039273 | None:intergenic | 45.0% | |
| CTCTGAGCAAGCGTAAACAT+TGG | + | chr6.2:1039252-1039271 | MS.gene053045:CDS | 45.0% | |
| GAAGCAAGTAGTAGGAATGG+TGG | + | chr6.2:1038788-1038807 | MS.gene053045:CDS | 45.0% | |
| GCACAAATTGTTGCCTCTAG+TGG | + | chr6.2:1039057-1039076 | MS.gene053045:intron | 45.0% | |
| TTGGATTTGCCGTGTGTTCT+TGG | + | chr6.2:1039157-1039176 | MS.gene053045:intron | 45.0% | |
| AATGCGAGCTCGAACTCTGT+AGG | + | chr6.2:1039338-1039357 | MS.gene053045:CDS | 50.0% | |
| ATTCTCAGACACCGTTGCAG+AGG | - | chr6.2:1039321-1039340 | None:intergenic | 50.0% | |
| GCGCCGCTGTTTAACAACAA+TGG | - | chr6.2:1038983-1039002 | None:intergenic | 50.0% | |
| TTCTCAGACACCGTTGCAGA+GGG | - | chr6.2:1039320-1039339 | None:intergenic | 50.0% | |
| ! | AGTGCTGCTATAGCCACTAG+AGG | - | chr6.2:1039073-1039092 | None:intergenic | 50.0% |
| TCAGACACCGTTGCAGAGGG+AGG | - | chr6.2:1039317-1039336 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 1038786 | 1039379 | 1038786 | ID=MS.gene053045 |
| chr6.2 | mRNA | 1038786 | 1039379 | 1038786 | ID=MS.gene053045.t1;Parent=MS.gene053045 |
| chr6.2 | exon | 1038786 | 1038947 | 1038786 | ID=MS.gene053045.t1.exon1;Parent=MS.gene053045.t1 |
| chr6.2 | CDS | 1038786 | 1038947 | 1038786 | ID=cds.MS.gene053045.t1;Parent=MS.gene053045.t1 |
| chr6.2 | exon | 1039230 | 1039379 | 1039230 | ID=MS.gene053045.t1.exon2;Parent=MS.gene053045.t1 |
| chr6.2 | CDS | 1039230 | 1039379 | 1039230 | ID=cds.MS.gene053045.t1;Parent=MS.gene053045.t1 |
| Gene Sequence |
| Protein sequence |
