AlfalfaGEDB | MS.gene43424

M. sativa cultivar XinJiangDaYe / MS.gene43424

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43424.t1	XP_013458655.1	96.3	136	5	0	1	136	1	136	3.10E-65	257.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43424.t1	O23310	76.1	117	22	2	1	113	1	115	6.3e-43	174.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43424.t1	I3TAW6	96.3	136	5	0	1	136	1	136	2.2e-65	257.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene43424.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene43424	0.805197	1.50E-49	-1.69E-46
MS.gene049499	MS.gene43424	0.822814	1.94E-53	-1.69E-46
MS.gene049983	MS.gene43424	0.825163	5.44E-54	-1.69E-46
MS.gene050414	MS.gene43424	0.829354	5.40E-55	-1.69E-46
MS.gene050559	MS.gene43424	0.810524	1.10E-50	-1.69E-46
MS.gene050561	MS.gene43424	0.806197	9.24E-50	-1.69E-46
MS.gene051279	MS.gene43424	0.804951	1.69E-49	-1.69E-46
MS.gene051911	MS.gene43424	0.803936	2.75E-49	-1.69E-46
MS.gene052954	MS.gene43424	0.808615	2.84E-50	-1.69E-46
MS.gene053272	MS.gene43424	-0.819891	9.15E-53	-1.69E-46
MS.gene053931	MS.gene43424	0.831721	1.42E-55	-1.69E-46
MS.gene055464	MS.gene43424	0.813695	2.25E-51	-1.69E-46
MS.gene05686	MS.gene43424	0.802714	4.92E-49	-1.69E-46
MS.gene057208	MS.gene43424	0.839516	1.51E-57	-1.69E-46
MS.gene058008	MS.gene43424	0.841112	5.79E-58	-1.69E-46
MS.gene058009	MS.gene43424	0.839194	1.83E-57	-1.69E-46
MS.gene058781	MS.gene43424	0.846182	2.55E-59	-1.69E-46
MS.gene058783	MS.gene43424	0.841092	5.86E-58	-1.69E-46
MS.gene05952	MS.gene43424	0.822112	2.82E-53	-1.69E-46
MS.gene059949	MS.gene43424	0.812361	4.41E-51	-1.69E-46
MS.gene060244	MS.gene43424	0.828871	7.06E-55	-1.69E-46
MS.gene060935	MS.gene43424	0.82665	2.41E-54	-1.69E-46
MS.gene060937	MS.gene43424	0.817806	2.72E-52	-1.69E-46
MS.gene061100	MS.gene43424	0.816146	6.43E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43424.t1	MTR_4g133938	96.324	136	5	0	1	136	1	136	1.70e-94	268
MS.gene43424.t1	MTR_2g026710	75.385	130	27	2	1	130	1	125	1.20e-64	194
MS.gene43424.t1	MTR_5g095740	75.000	120	26	2	1	116	1	120	1.48e-59	182
MS.gene43424.t1	MTR_3g058980	71.901	121	26	1	1	113	1	121	4.29e-59	181
MS.gene43424.t1	MTR_1g072790	70.588	119	32	2	10	127	22	138	6.82e-55	170
MS.gene43424.t1	MTR_8g091720	81.443	97	18	0	20	116	29	125	1.01e-54	170
MS.gene43424.t1	MTR_8g093920	79.787	94	19	0	20	113	41	134	2.84e-54	169
MS.gene43424.t1	MTR_0392s0020	68.182	110	33	1	6	113	3	112	6.33e-53	164
MS.gene43424.t1	MTR_4g119500	68.182	110	30	1	4	113	12	116	7.38e-53	163
MS.gene43424.t1	MTR_7g061270	65.079	126	41	1	7	129	14	139	2.02e-52	163
MS.gene43424.t1	MTR_7g100650	65.079	126	41	1	7	129	14	139	3.42e-52	163
MS.gene43424.t1	MTR_1g088860	74.468	94	24	0	20	113	37	130	1.33e-51	162
MS.gene43424.t1	MTR_4g133952	67.021	94	31	0	20	113	59	152	1.62e-45	148
MS.gene43424.t1	MTR_1g083070	54.867	113	45	1	21	133	6	112	4.20e-42	135
MS.gene43424.t1	MTR_1g039040	57.426	101	41	1	13	113	2	100	6.87e-40	132
MS.gene43424.t1	MTR_1g029070	53.261	92	43	0	20	111	53	144	1.42e-28	103
MS.gene43424.t1	MTR_5g095900	46.667	105	51	2	7	108	3	105	4.75e-25	94.4
MS.gene43424.t1	MTR_1g028480	51.515	66	32	0	49	114	19	84	6.23e-19	76.6
MS.gene43424.t1	MTR_1g029100	40.789	76	45	0	24	99	7	82	6.66e-19	75.9
MS.gene43424.t1	MTR_2g056000	30.952	84	58	0	24	107	46	129	2.23e-13	63.9
MS.gene43424.t1	MTR_4g112380	30.952	84	58	0	24	107	16	99	3.91e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43424.t1	AT4G14540	75.424	118	23	2	1	114	1	116	2.79e-60	183
MS.gene43424.t1	AT5G47640	82.105	95	17	0	20	114	28	122	4.60e-55	171
MS.gene43424.t1	AT2G38880	65.323	124	39	3	9	130	12	133	4.16e-52	162
MS.gene43424.t1	AT2G47810	76.596	94	22	0	20	113	52	145	4.69e-52	162
MS.gene43424.t1	AT2G38880	65.323	124	39	3	9	130	12	133	6.09e-52	161
MS.gene43424.t1	AT2G38880	65.323	124	39	3	9	130	12	133	6.09e-52	161
MS.gene43424.t1	AT2G38880	65.323	124	39	3	9	130	12	133	6.09e-52	161
MS.gene43424.t1	AT2G13570	76.596	94	22	0	20	113	37	130	1.86e-51	162
MS.gene43424.t1	AT3G53340	74.194	93	24	0	20	112	30	122	2.89e-50	158
MS.gene43424.t1	AT3G53340	74.194	93	24	0	20	112	30	122	2.89e-50	158
MS.gene43424.t1	AT2G37060	69.725	109	29	2	7	112	16	123	3.51e-50	158
MS.gene43424.t1	AT2G37060	69.725	109	29	2	7	112	16	123	3.51e-50	158
MS.gene43424.t1	AT2G37060	69.725	109	29	2	7	112	16	123	3.51e-50	158
MS.gene43424.t1	AT2G38880	72.549	102	25	2	9	109	12	111	1.14e-49	154
MS.gene43424.t1	AT2G38880	72.549	102	25	2	9	109	12	111	2.12e-49	155
MS.gene43424.t1	AT2G38880	72.549	102	25	2	9	109	12	111	2.12e-49	155
MS.gene43424.t1	AT2G38880	72.549	102	25	2	9	109	12	111	2.12e-49	155
MS.gene43424.t1	AT2G38880	63.415	123	41	3	9	130	12	131	3.37e-49	154
MS.gene43424.t1	AT2G38880	72.549	102	25	2	9	109	12	111	4.56e-49	155
MS.gene43424.t1	AT2G38880	72.549	102	25	2	9	109	12	111	4.56e-49	155
MS.gene43424.t1	AT3G53340	73.333	90	24	0	20	109	30	119	1.03e-48	152
MS.gene43424.t1	AT3G53340	73.626	91	24	0	20	110	30	120	3.17e-48	152
MS.gene43424.t1	AT3G53340	73.626	91	24	0	20	110	30	120	3.17e-48	152
MS.gene43424.t1	AT5G47670	63.918	97	35	0	20	116	30	126	6.22e-45	145
MS.gene43424.t1	AT5G47670	63.918	97	35	0	20	116	59	155	1.37e-44	145
MS.gene43424.t1	AT5G47670	63.918	97	35	0	20	116	59	155	1.37e-44	145
MS.gene43424.t1	AT1G09030	57.391	115	46	1	20	131	4	118	2.09e-44	142
MS.gene43424.t1	AT1G21970	58.163	98	41	0	20	117	60	157	2.81e-41	137
MS.gene43424.t1	AT5G08190	35.052	97	61	1	24	118	16	112	7.29e-18	75.5
MS.gene43424.t1	AT5G08190	34.694	98	61	2	24	118	16	113	4.08e-16	70.9
MS.gene43424.t1	AT5G23090	30.928	97	65	1	24	118	16	112	8.68e-15	67.4
MS.gene43424.t1	AT5G23090	34.118	85	54	1	24	106	16	100	5.56e-14	65.1
MS.gene43424.t1	AT5G23090	34.118	85	54	1	24	106	16	100	5.56e-14	65.1
MS.gene43424.t1	AT5G23090	34.118	85	54	1	24	106	16	100	5.56e-14	65.1
MS.gene43424.t1	AT5G23090	32.941	85	54	2	25	106	3	87	7.86e-11	56.6

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATCAGGTGGTCAAGCTTC+AGG	0.278269	8.4:-814207	MS.gene43424:CDS
GGGTTACCTTCACAAGTATA+GGG	0.382046	8.4:-813926	MS.gene43424:CDS
GATCATGAAGAAAGCTTTAC+CGG	0.392387	8.4:-814133	MS.gene43424:CDS
AGAATATGCAGAGCCTCTTA+AGG	0.393028	8.4:-813947	MS.gene43424:CDS
AGGGTTACCTTCACAAGTAT+AGG	0.429699	8.4:-813927	MS.gene43424:CDS
TTCGATATCTTTGCTCTCGC+CGG	0.458504	8.4:+814114	None:intergenic
GAGTTCATAAGCTTCATAAC+AGG	0.460674	8.4:-814057	MS.gene43424:CDS
GATCCTACCAACGTTTGCTA+TGG	0.475579	8.4:+814152	None:intergenic
GCAGAATCAGATGATGAATC+AGG	0.475967	8.4:-814222	MS.gene43424:CDS
GAGAAGAGGAAGACAATAAA+CGG	0.477283	8.4:-814009	MS.gene43424:CDS
TTGTGAAGGTAACCCTTAAG+AGG	0.483388	8.4:+813934	None:intergenic
CAATTTCCCTATACTTGTGA+AGG	0.485169	8.4:+813920	None:intergenic
GAATATGCAGAGCCTCTTAA+GGG	0.488554	8.4:-813946	MS.gene43424:CDS
AATCAGGTGGTCAAGCTTCA+GGG	0.501937	8.4:-814206	MS.gene43424:CDS
GAATATGATTGGTAGTAGTA+AGG	0.514281	8.4:-813875	MS.gene43424:CDS
GCAGAGAATTACTGCAAGAA+AGG	0.517934	8.4:-814182	MS.gene43424:CDS
CACAAGTATAGGGAAATTGA+AGG	0.531636	8.4:-813916	MS.gene43424:CDS
GTTGCAAATATAATCAACCT+TGG	0.534009	8.4:+813824	None:intergenic
ATCCTACCAACGTTTGCTAT+GGG	0.537868	8.4:+814153	None:intergenic
AAAAGTGTCAGAAAGAGAAG+AGG	0.551950	8.4:-814023	MS.gene43424:CDS
AGTATTGATCGATTCTTCCA+AGG	0.558864	8.4:-813841	MS.gene43424:CDS
TTCAAAGCCAAGTGTAGTCA+TGG	0.563942	8.4:+813969	None:intergenic
GTGTGGGCCATGACTACACT+TGG	0.565546	8.4:-813976	MS.gene43424:CDS
TACCAACGTTTGCTATGGGA+AGG	0.599059	8.4:+814157	None:intergenic
TTCCCATAGCAAACGTTGGT+AGG	0.621000	8.4:-814155	MS.gene43424:CDS
CTCCTTCCCATAGCAAACGT+TGG	0.626066	8.4:-814159	MS.gene43424:CDS
GGTAGTAGTAAGGAGCAGCA+AGG	0.645799	8.4:-813865	MS.gene43424:CDS
TAAACGGTGATGATCTTGTG+TGG	0.661494	8.4:-813993	MS.gene43424:CDS
AAACGGTGATGATCTTGTGT+GGG	0.691215	8.4:-813992	MS.gene43424:CDS
GAATCAGATGATGAATCAGG+TGG	0.704177	8.4:-814219	MS.gene43424:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTTCTATGAATATGAT+TGG	-	chr8.4:814176-814195	MS.gene43424:CDS	15.0%
!	GAATATGATTGGTAGTAGTA+AGG	-	chr8.4:814187-814206	MS.gene43424:CDS	30.0%
	CAATTTCCCTATACTTGTGA+AGG	+	chr8.4:814145-814164	None:intergenic	35.0%
	CACAAGTATAGGGAAATTGA+AGG	-	chr8.4:814146-814165	MS.gene43424:CDS	35.0%
	GAGAAGAGGAAGACAATAAA+CGG	-	chr8.4:814053-814072	MS.gene43424:CDS	35.0%
	GAGTTCATAAGCTTCATAAC+AGG	-	chr8.4:814005-814024	MS.gene43424:CDS	35.0%
	GATCATGAAGAAAGCTTTAC+CGG	-	chr8.4:813929-813948	MS.gene43424:CDS	35.0%
!!	AAAAGTGTCAGAAAGAGAAG+AGG	-	chr8.4:814039-814058	MS.gene43424:CDS	35.0%
!!	AGTATTGATCGATTCTTCCA+AGG	-	chr8.4:814221-814240	MS.gene43424:CDS	35.0%
	AAACGGTGATGATCTTGTGT+GGG	-	chr8.4:814070-814089	MS.gene43424:CDS	40.0%
	AGAATATGCAGAGCCTCTTA+AGG	-	chr8.4:814115-814134	MS.gene43424:CDS	40.0%
	AGGGTTACCTTCACAAGTAT+AGG	-	chr8.4:814135-814154	MS.gene43424:CDS	40.0%
	GAATATGCAGAGCCTCTTAA+GGG	-	chr8.4:814116-814135	MS.gene43424:CDS	40.0%
	GAATCAGATGATGAATCAGG+TGG	-	chr8.4:813843-813862	MS.gene43424:CDS	40.0%
	GCAGAATCAGATGATGAATC+AGG	-	chr8.4:813840-813859	MS.gene43424:CDS	40.0%
	GCAGAGAATTACTGCAAGAA+AGG	-	chr8.4:813880-813899	MS.gene43424:CDS	40.0%
	GGGTTACCTTCACAAGTATA+GGG	-	chr8.4:814136-814155	MS.gene43424:CDS	40.0%
	TAAACGGTGATGATCTTGTG+TGG	-	chr8.4:814069-814088	MS.gene43424:CDS	40.0%
	TTCAAAGCCAAGTGTAGTCA+TGG	+	chr8.4:814096-814115	None:intergenic	40.0%
	TTGTGAAGGTAACCCTTAAG+AGG	+	chr8.4:814131-814150	None:intergenic	40.0%
!!	ATCCTACCAACGTTTGCTAT+GGG	+	chr8.4:813912-813931	None:intergenic	40.0%
	AATCAGGTGGTCAAGCTTCA+GGG	-	chr8.4:813856-813875	MS.gene43424:CDS	45.0%
	TTCCCATAGCAAACGTTGGT+AGG	-	chr8.4:813907-813926	MS.gene43424:CDS	45.0%
	TTCGATATCTTTGCTCTCGC+CGG	+	chr8.4:813951-813970	None:intergenic	45.0%
!	GATCCTACCAACGTTTGCTA+TGG	+	chr8.4:813913-813932	None:intergenic	45.0%
!!	TACCAACGTTTGCTATGGGA+AGG	+	chr8.4:813908-813927	None:intergenic	45.0%
	CTCCTTCCCATAGCAAACGT+TGG	-	chr8.4:813903-813922	MS.gene43424:CDS	50.0%
	GAATCAGGTGGTCAAGCTTC+AGG	-	chr8.4:813855-813874	MS.gene43424:CDS	50.0%
	GGTAGTAGTAAGGAGCAGCA+AGG	-	chr8.4:814197-814216	MS.gene43424:CDS	50.0%
	GTGTGGGCCATGACTACACT+TGG	-	chr8.4:814086-814105	MS.gene43424:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	813837	814247	813837	ID=MS.gene43424
chr8.4	mRNA	813837	814247	813837	ID=MS.gene43424.t1;Parent=MS.gene43424
chr8.4	exon	813837	814247	813837	ID=MS.gene43424.t1.exon1;Parent=MS.gene43424.t1
chr8.4	CDS	813837	814247	813837	ID=cds.MS.gene43424.t1;Parent=MS.gene43424.t1

Gene Sequence

>MS.gene43424

ATGGCAGAATCAGATGATGAATCAGGTGGTCAAGCTTCAGGGAGCAGAGAATTACTGCAAGAAAGGCTCCTTCCCATAGCAAACGTTGGTAGGATCATGAAGAAAGCTTTACCGGCGAGAGCAAAGATATCGAAAGAAGCTAAAGAAACGATGCAAGAGTGTGTATCAGAGTTCATAAGCTTCATAACAGGTGAAGCATCTGAAAAGTGTCAGAAAGAGAAGAGGAAGACAATAAACGGTGATGATCTTGTGTGGGCCATGACTACACTTGGCTTTGAAGAATATGCAGAGCCTCTTAAGGGTTACCTTCACAAGTATAGGGAAATTGAAGGTGACAAGAATTTTTCTATGAATATGATTGGTAGTAGTAAGGAGCAGCAAGGTAGTATTGATCGATTCTTCCAAGGTTGA

Protein sequence

>MS.gene43424.t1

MAESDDESGGQASGSRELLQERLLPIANVGRIMKKALPARAKISKEAKETMQECVSEFISFITGEASEKCQKEKRKTINGDDLVWAMTTLGFEEYAEPLKGYLHKYREIEGDKNFSMNMIGSSKEQQGSIDRFFQG