AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43604

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43604.t1 MTR_2g018260 91.593 226 18 1 1 226 1 225 3.02e-155 429
MS.gene43604.t1 MTR_1g045130 51.977 177 82 2 51 226 31 205 8.68e-63 194
MS.gene43604.t1 MTR_6g029230 35.220 159 101 1 41 199 40 196 4.14e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43604.t1 AT4G18040 62.397 242 68 2 1 226 1 235 6.06e-105 303
MS.gene43604.t1 AT1G29550 60.386 207 81 1 20 226 35 240 1.02e-90 267
MS.gene43604.t1 AT1G29590 59.903 207 82 1 20 226 35 240 4.89e-90 265
MS.gene43604.t1 AT5G35620 49.457 184 85 6 38 215 12 193 3.00e-55 175
MS.gene43604.t1 AT5G35620 49.457 184 85 6 38 215 12 193 3.00e-55 175
MS.gene43604.t1 AT5G35620 47.531 162 77 6 31 186 5 164 3.36e-44 146
MS.gene43604.t1 AT5G18110 35.570 149 96 0 51 199 43 191 6.66e-28 105

Find 54 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAATTCTTGGACTTTC+TGG 0.032991 2.2:+66330707 MS.gene43604:CDS
CAAATAGGGTCTTCCCATTT+TGG 0.162249 2.2:-66331830 None:intergenic
CTCACCCTCTTGAGAATTCT+TGG 0.186379 2.2:+66330698 MS.gene43604:CDS
AGCATAAGATTGAACCAAAA+TGG 0.214116 2.2:+66331816 MS.gene43604:CDS
TTTCTACTGTTGAAGAGTTT+TGG 0.243222 2.2:+66330797 MS.gene43604:CDS
AGGCGGAAAGAATTTCTATT+TGG 0.271159 2.2:+66332098 MS.gene43604:CDS
TTTCCACCATTAGCACAAAT+AGG 0.313839 2.2:-66331845 None:intergenic
GTGACCGGCGGTGAGGGGTT+TGG 0.329982 2.2:-66330671 None:intergenic
CTTTAGTTGTTGGCAATGAT+TGG 0.357757 2.2:+66332019 MS.gene43604:intron
ACTACCCCAAGGTTTACTTT+TGG 0.361171 2.2:-66330752 None:intergenic
TTTGTGCTAATGGTGGAAAT+TGG 0.362454 2.2:+66331849 MS.gene43604:CDS
AGATTGGTGACCGGCGGTGA+GGG 0.372856 2.2:-66330677 None:intergenic
TGTGATTTGATCGTCGTTGA+TGG 0.385098 2.2:-66330575 None:intergenic
TAAATCCAAAAGTAAACCTT+GGG 0.396453 2.2:+66330747 MS.gene43604:CDS
TTCCACCATTAGCACAAATA+GGG 0.400308 2.2:-66331844 None:intergenic
GGCGGTGAGGGGTTTGGAAA+TGG 0.408337 2.2:-66330665 None:intergenic
TGATACTAGCTGGTTGTATA+CGG 0.414513 2.2:+66331898 MS.gene43604:CDS
AATTCTCAAGAGGGTGAGAT+TGG 0.439429 2.2:-66330693 None:intergenic
TGCTGTTAATGAAGCTGCTC+AGG 0.445927 2.2:+66332129 MS.gene43604:CDS
CTTGATTATAATGAAACCAT+TGG 0.456939 2.2:+66333131 MS.gene43604:CDS
GTGGAGCAGTTGTGAATGTC+AGG 0.457663 2.2:+66332071 MS.gene43604:CDS
GCATAAGATTGAACCAAAAT+GGG 0.459310 2.2:+66331817 MS.gene43604:CDS
ATTGGAGAGCAATTTGATCA+TGG 0.466010 2.2:+66332037 MS.gene43604:CDS
GATCATGGGGTTGAAATTTG+TGG 0.470727 2.2:+66332052 MS.gene43604:CDS
AGTTGTGAATGTCAGGGATA+AGG 0.499701 2.2:+66332078 MS.gene43604:CDS
TTGGAGAGCAATTTGATCAT+GGG 0.505267 2.2:+66332038 MS.gene43604:CDS
GCTCGAACAGCCAACTTGCT+AGG 0.505369 2.2:-66331776 None:intergenic
GAAGACCCTATTTGTGCTAA+TGG 0.505377 2.2:+66331839 MS.gene43604:CDS
GGTGAGGGGTTTGGAAATGG+CGG 0.516008 2.2:-66330662 None:intergenic
GTTATATTTCAGATGAGCAT+CGG 0.524149 2.2:+66333089 MS.gene43604:intron
AACAGTAGAAATAGTGTAGA+CGG 0.531049 2.2:-66330785 None:intergenic
GGGGAAAATCTGATACTAGC+TGG 0.537034 2.2:+66331888 MS.gene43604:CDS
CTAAATCCAAAAGTAAACCT+TGG 0.540172 2.2:+66330746 MS.gene43604:CDS
ATCAACAACGATCTCGAAGA+AGG 0.542418 2.2:+66330616 MS.gene43604:CDS
GAACAGCCAACTTGCTAGGA+TGG 0.556707 2.2:-66331772 None:intergenic
GATTGGTGACCGGCGGTGAG+GGG 0.563209 2.2:-66330676 None:intergenic
GAGATTGGTGACCGGCGGTG+AGG 0.563789 2.2:-66330678 None:intergenic
TACATGAAATATAAAGCCAA+TGG 0.568932 2.2:-66333147 None:intergenic
TATTCACCATCCTAGCAAGT+TGG 0.570667 2.2:+66331766 MS.gene43604:CDS
ATTTCCAAACCCCTCACCGC+CGG 0.575370 2.2:+66330667 MS.gene43604:CDS
ACAGTAGAAATAGTGTAGAC+GGG 0.593157 2.2:-66330784 None:intergenic
GAGGGGTTTGGAAATGGCGG+AGG 0.595654 2.2:-66330659 None:intergenic
GAGCATCGGAAAACAATGGA+AGG 0.597596 2.2:+66333103 MS.gene43604:CDS
GACCCTATTTGTGCTAATGG+TGG 0.605399 2.2:+66331842 MS.gene43604:CDS
AAGAGGGTGAGATTGGTGAC+CGG 0.612893 2.2:-66330686 None:intergenic
AGATGAGCATCGGAAAACAA+TGG 0.613189 2.2:+66333099 MS.gene43604:intron
TGGAGAGCAATTTGATCATG+GGG 0.619622 2.2:+66332039 MS.gene43604:CDS
AAAGTCCAAGAATTCTCAAG+AGG 0.638231 2.2:-66330703 None:intergenic
AAGTCCAAGAATTCTCAAGA+GGG 0.640086 2.2:-66330702 None:intergenic
TGTGAATGTCAGGGATAAGG+CGG 0.669319 2.2:+66332081 MS.gene43604:CDS
AGGGTGAGATTGGTGACCGG+CGG 0.679762 2.2:-66330683 None:intergenic
TGGAGCAGTTGTGAATGTCA+GGG 0.698604 2.2:+66332072 MS.gene43604:CDS
GGTCGAATCGAACTACCCCA+AGG 0.701179 2.2:-66330763 None:intergenic
AAATCCAAAAGTAAACCTTG+GGG 0.727654 2.2:+66330748 MS.gene43604:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGTTGAAGAATTTTTT+AGG + chr2.2:66331023-66331042 MS.gene43604:intron 15.0%
!!! AAGATTCAATTTTTATGAAT+TGG + chr2.2:66331326-66331345 MS.gene43604:intron 15.0%
!!! AAGATTCATTTTTTATTTCT+TGG + chr2.2:66330897-66330916 MS.gene43604:intron 15.0%
!!! AGATTCAATTTTTATGAATT+GGG + chr2.2:66331327-66331346 MS.gene43604:intron 15.0%
!!! AGATTCATTTTTTATTTCTT+GGG + chr2.2:66330898-66330917 MS.gene43604:intron 15.0%
!!! TATGATTCACTATTATTTTT+TGG + chr2.2:66332864-66332883 MS.gene43604:intron 15.0%
!!! TGATTCATTTTTAGTTAATT+GGG + chr2.2:66330986-66331005 MS.gene43604:intron 15.0%
!! AATTGTAAACAATAAGGTAT+TGG - chr2.2:66332383-66332402 None:intergenic 20.0%
!! AGATTGAATTGTAAACAATA+AGG - chr2.2:66332389-66332408 None:intergenic 20.0%
!! ATAAACAGATACATAATTTC+TGG - chr2.2:66332997-66333016 None:intergenic 20.0%
!! ATTTCTATAAGCTATATTTG+AGG + chr2.2:66331224-66331243 MS.gene43604:intron 20.0%
!! GAATTAGATGGTTATTATAA+TGG + chr2.2:66332271-66332290 MS.gene43604:intron 20.0%
!! GATTGAGAATTTATATTTCA+GGG + chr2.2:66332771-66332790 MS.gene43604:intron 20.0%
!! TGATTGAGAATTTATATTTC+AGG + chr2.2:66332770-66332789 MS.gene43604:intron 20.0%
!! TTCCATATAATTTACTCTTA+TGG + chr2.2:66332962-66332981 MS.gene43604:intron 20.0%
!!! AATTCAATCTTTTCAACTTT+AGG + chr2.2:66331635-66331654 MS.gene43604:intron 20.0%
!!! AATTGGGTATTTTTTACATT+AGG + chr2.2:66330958-66330977 MS.gene43604:intron 20.0%
!!! ACAATGTTGAAGAATTTTTT+AGG + chr2.2:66331407-66331426 MS.gene43604:intron 20.0%
!!! ACTATTGAATTTGTTTTATG+AGG + chr2.2:66331924-66331943 MS.gene43604:intron 20.0%
!!! ACTGATTATACTTTTGATAA+TGG + chr2.2:66333043-66333062 MS.gene43604:intron 20.0%
!!! ATTGGGTATTTTTTACATTA+GGG + chr2.2:66330959-66330978 MS.gene43604:intron 20.0%
!!! ATTGTAAACAATAAGGTATT+GGG - chr2.2:66332382-66332401 None:intergenic 20.0%
!!! ATTGTTCATTTATTGATTCT+TGG + chr2.2:66331678-66331697 MS.gene43604:intron 20.0%
!!! CTATTGAATTTGTTTTATGA+GGG + chr2.2:66331925-66331944 MS.gene43604:intron 20.0%
!!! CTGATTATACTTTTGATAAT+GGG + chr2.2:66333044-66333063 MS.gene43604:intron 20.0%
!!! GTGATTCATTTTTAGTTAAT+TGG + chr2.2:66330985-66331004 MS.gene43604:intron 20.0%
!!! TGATGAATTAAAGTACTAAT+TGG + chr2.2:66331969-66331988 MS.gene43604:intron 20.0%
!!! TGATTATACTTTTGATAATG+GGG + chr2.2:66333045-66333064 MS.gene43604:intron 20.0%
! AACCATCTAATTCTTTCTTT+GGG - chr2.2:66332264-66332283 None:intergenic 25.0%
! ACAATTCAAAACAGAAGTTA+GGG - chr2.2:66333238-66333257 None:intergenic 25.0%
! ACTAAATTGATAGACCAAAA+AGG - chr2.2:66332733-66332752 None:intergenic 25.0%
! AGCCATAAGAGTAAATTATA+TGG - chr2.2:66332967-66332986 None:intergenic 25.0%
! CAGTTCATGAACAATTTAAA+TGG + chr2.2:66332308-66332327 MS.gene43604:intron 25.0%
! CTTGATTATAATGAAACCAT+TGG + chr2.2:66333131-66333150 MS.gene43604:CDS 25.0%
! TAAATCCAAAAGTAAACCTT+GGG + chr2.2:66330747-66330766 MS.gene43604:CDS 25.0%
! TAACCATCTAATTCTTTCTT+TGG - chr2.2:66332265-66332284 None:intergenic 25.0%
! TACATGAAATATAAAGCCAA+TGG - chr2.2:66333150-66333169 None:intergenic 25.0%
! TATGCAATCTTATACAAAAG+CGG - chr2.2:66331145-66331164 None:intergenic 25.0%
! TGACAAATATAAAAACACAC+CGG - chr2.2:66333200-66333219 None:intergenic 25.0%
! TTTATAGACTTTGGTCAATT+TGG + chr2.2:66332646-66332665 MS.gene43604:intron 25.0%
! TTTGAGGACTTATGAAAATA+AGG + chr2.2:66331240-66331259 MS.gene43604:intron 25.0%
!! AAAATGTTGCAGAAATTTTG+AGG + chr2.2:66331608-66331627 MS.gene43604:intron 25.0%
!! AAGATTCAGTTTTTGTGAAT+TGG + chr2.2:66330941-66330960 MS.gene43604:intron 25.0%
!! AATTGGGTATTTTTCACATT+AGG + chr2.2:66331343-66331362 MS.gene43604:intron 25.0%
!! AGATTCAGTTTTTGTGAATT+GGG + chr2.2:66330942-66330961 MS.gene43604:intron 25.0%
!! ATCGTTCATTTATTGTTTCT+TGG + chr2.2:66331553-66331572 MS.gene43604:intron 25.0%
!! ATTGGGTATTTTTCACATTA+GGG + chr2.2:66331344-66331363 MS.gene43604:intron 25.0%
!! GTTTTTCATCAATACCTTAT+TGG + chr2.2:66332592-66332611 MS.gene43604:intron 25.0%
!! TTTTCAACTTTAGGTCTTTT+GGG + chr2.2:66331644-66331663 MS.gene43604:intron 25.0%
!!! AATTGTTCATTTGTTGTTTC+TGG + chr2.2:66330868-66330887 MS.gene43604:intron 25.0%
!!! AGTTTTAAAATTGCTGAGTT+AGG - chr2.2:66332839-66332858 None:intergenic 25.0%
!!! ATTGTTCATTTGTTGTTTCT+GGG + chr2.2:66330869-66330888 MS.gene43604:intron 25.0%
!!! CTTGGTTATTTTTGTATCTT+GGG + chr2.2:66331571-66331590 MS.gene43604:intron 25.0%
!!! GTTCTTACTTTTGATTCAAT+AGG + chr2.2:66332161-66332180 MS.gene43604:intron 25.0%
!!! TCGTGTTACTGAAATTTTTA+TGG + chr2.2:66331722-66331741 MS.gene43604:intron 25.0%
!!! TCTTGGGTGTTTTTAATTTT+GGG + chr2.2:66330914-66330933 MS.gene43604:intron 25.0%
!!! TCTTGGTTATTTTTGTATCT+TGG + chr2.2:66331570-66331589 MS.gene43604:intron 25.0%
!!! TTCTTGGGTGTTTTTAATTT+TGG + chr2.2:66330913-66330932 MS.gene43604:intron 25.0%
AAATCCAAAAGTAAACCTTG+GGG + chr2.2:66330748-66330767 MS.gene43604:CDS 30.0%
AACAGTAGAAATAGTGTAGA+CGG - chr2.2:66330788-66330807 None:intergenic 30.0%
AATAAGCTCTTTATCCAAAC+AGG - chr2.2:66331077-66331096 None:intergenic 30.0%
ACCATCTAATTCTTTCTTTG+GGG - chr2.2:66332263-66332282 None:intergenic 30.0%
AGCATAAGATTGAACCAAAA+TGG + chr2.2:66331816-66331835 MS.gene43604:CDS 30.0%
CACAATTCAAAACAGAAGTT+AGG - chr2.2:66333239-66333258 None:intergenic 30.0%
CTAAATCCAAAAGTAAACCT+TGG + chr2.2:66330746-66330765 MS.gene43604:CDS 30.0%
GCATAAGATTGAACCAAAAT+GGG + chr2.2:66331817-66331836 MS.gene43604:CDS 30.0%
GTTATATTTCAGATGAGCAT+CGG + chr2.2:66333089-66333108 MS.gene43604:intron 30.0%
TCTCAGAAGTTTATAGACTT+TGG + chr2.2:66332637-66332656 MS.gene43604:intron 30.0%
TGATGAAAAACAAGAAACAC+AGG - chr2.2:66332583-66332602 None:intergenic 30.0%
TTGATTCAATAGGAACTAGA+TGG + chr2.2:66332171-66332190 MS.gene43604:intron 30.0%
! AACTCTTGTAATTTGTCAGA+TGG + chr2.2:66332901-66332920 MS.gene43604:intron 30.0%
! AGCTTCAATCTTTTCACTTT+AGG + chr2.2:66331049-66331068 MS.gene43604:intron 30.0%
! CTTTTCAACTTTAGGTCTTT+TGG + chr2.2:66331643-66331662 MS.gene43604:intron 30.0%
! GATTCTATCCTTTTCACTTT+TGG + chr2.2:66330827-66330846 MS.gene43604:intron 30.0%
! GCTTCAATCTTTTCACTTTA+GGG + chr2.2:66331050-66331069 MS.gene43604:intron 30.0%
! GGCTTTATATTTCATGTAAG+TGG + chr2.2:66333152-66333171 MS.gene43604:intron 30.0%
! TGGAAAGTTTTCACGTTTTT+TGG + chr2.2:66332547-66332566 MS.gene43604:intron 30.0%
! TGTTGATGTCTTCGATTTTA+GGG - chr2.2:66330603-66330622 None:intergenic 30.0%
! TTCTTGGTAACTTTAGTTGT+TGG + chr2.2:66332009-66332028 MS.gene43604:intron 30.0%
! TTGAGGATTTTCAATATCAG+AGG - chr2.2:66332701-66332720 None:intergenic 30.0%
! TTGTTGATGTCTTCGATTTT+AGG - chr2.2:66330604-66330623 None:intergenic 30.0%
! TTTCTACTGTTGAAGAGTTT+TGG + chr2.2:66330797-66330816 MS.gene43604:CDS 30.0%
!! AACTTCTGAGACTATATTAG+TGG - chr2.2:66332628-66332647 None:intergenic 30.0%
!! AATCTTTTGAGAGACTGTTT+AGG - chr2.2:66331312-66331331 None:intergenic 30.0%
!! TTGAATTGTGCTATTTCTGT+AGG + chr2.2:66333249-66333268 MS.gene43604:intron 30.0%
!!! TACTTTTGGATTTAGCTTGA+GGG - chr2.2:66330741-66330760 None:intergenic 30.0%
!!! TTACTTTTGGATTTAGCTTG+AGG - chr2.2:66330742-66330761 None:intergenic 30.0%
AAAGTCCAAGAATTCTCAAG+AGG - chr2.2:66330706-66330725 None:intergenic 35.0%
AAGTCCAAGAATTCTCAAGA+GGG - chr2.2:66330705-66330724 None:intergenic 35.0%
ACAGTAGAAATAGTGTAGAC+GGG - chr2.2:66330787-66330806 None:intergenic 35.0%
ACCCCAAAGAAAGAATTAGA+TGG + chr2.2:66332259-66332278 MS.gene43604:intron 35.0%
AGCATGTATTCACAGTTCAT+CGG + chr2.2:66332515-66332534 MS.gene43604:intron 35.0%
AGGCGGAAAGAATTTCTATT+TGG + chr2.2:66332098-66332117 MS.gene43604:CDS 35.0%
ATGAGCAGTTGTTATGTGAA+TGG + chr2.2:66332455-66332474 MS.gene43604:intron 35.0%
ATTGGAGAGCAATTTGATCA+TGG + chr2.2:66332037-66332056 MS.gene43604:CDS 35.0%
CAAAAAGGTCTAACTGATTG+AGG - chr2.2:66332718-66332737 None:intergenic 35.0%
CTTTAGTTGTTGGCAATGAT+TGG + chr2.2:66332019-66332038 MS.gene43604:intron 35.0%
GAGGACTTATGAAAATAAGG+TGG + chr2.2:66331243-66331262 MS.gene43604:intron 35.0%
GTACTAATTGGTCATGTATG+TGG + chr2.2:66331981-66332000 MS.gene43604:intron 35.0%
TGATACTAGCTGGTTGTATA+CGG + chr2.2:66331898-66331917 MS.gene43604:CDS 35.0%
TTCCACCATTAGCACAAATA+GGG - chr2.2:66331847-66331866 None:intergenic 35.0%
TTGAGAATTCTTGGACTTTC+TGG + chr2.2:66330707-66330726 MS.gene43604:CDS 35.0%
TTGGAGAGCAATTTGATCAT+GGG + chr2.2:66332038-66332057 MS.gene43604:CDS 35.0%
TTTCCACCATTAGCACAAAT+AGG - chr2.2:66331848-66331867 None:intergenic 35.0%
! AGCTTCGATCTTTTCACTTT+AGG + chr2.2:66331433-66331452 MS.gene43604:intron 35.0%
! ATTGGACTGTGCAATTTTCT+AGG + chr2.2:66331867-66331886 MS.gene43604:CDS 35.0%
! CAGTTCATCGGCTATTTAAA+TGG + chr2.2:66332527-66332546 MS.gene43604:intron 35.0%
! CTCAATCAGTTAGACCTTTT+TGG + chr2.2:66332716-66332735 MS.gene43604:intron 35.0%
! GCTTCGATCTTTTCACTTTA+GGG + chr2.2:66331434-66331453 MS.gene43604:intron 35.0%
! TTGGACTGTGCAATTTTCTA+GGG + chr2.2:66331868-66331887 MS.gene43604:CDS 35.0%
! TTTGTGCTAATGGTGGAAAT+TGG + chr2.2:66331849-66331868 MS.gene43604:CDS 35.0%
!!! CATGTATGTGGTTTTGTTCT+TGG + chr2.2:66331993-66332012 MS.gene43604:intron 35.0%
AATTCTCAAGAGGGTGAGAT+TGG - chr2.2:66330696-66330715 None:intergenic 40.0%
ACATAACAACTGCTCATCGT+TGG - chr2.2:66332452-66332471 None:intergenic 40.0%
AGTTGTGAATGTCAGGGATA+AGG + chr2.2:66332078-66332097 MS.gene43604:CDS 40.0%
ATCAACAACGATCTCGAAGA+AGG + chr2.2:66330616-66330635 MS.gene43604:CDS 40.0%
CCAGAAGACCAAAAGTGAAA+AGG - chr2.2:66330838-66330857 None:intergenic 40.0%
GAAGACCCTATTTGTGCTAA+TGG + chr2.2:66331839-66331858 MS.gene43604:CDS 40.0%
GATCATGGGGTTGAAATTTG+TGG + chr2.2:66332052-66332071 MS.gene43604:CDS 40.0%
GTATCTGTTTATGCTTGTGC+TGG + chr2.2:66333005-66333024 MS.gene43604:intron 40.0%
GTGGTTCACTCTAACCAATA+AGG - chr2.2:66332609-66332628 None:intergenic 40.0%
TATTCACCATCCTAGCAAGT+TGG + chr2.2:66331766-66331785 MS.gene43604:CDS 40.0%
TGGAGAGCAATTTGATCATG+GGG + chr2.2:66332039-66332058 MS.gene43604:CDS 40.0%
TGGGAGAAAACAAGAAACAC+AGG - chr2.2:66332363-66332382 None:intergenic 40.0%
TGTGATTTGATCGTCGTTGA+TGG - chr2.2:66330578-66330597 None:intergenic 40.0%
! ACTACCCCAAGGTTTACTTT+TGG - chr2.2:66330755-66330774 None:intergenic 40.0%
! CAAATAGGGTCTTCCCATTT+TGG - chr2.2:66331833-66331852 None:intergenic 40.0%
! TGGACTGTGCAATTTTCTAG+GGG + chr2.2:66331869-66331888 MS.gene43604:CDS 40.0%
! TTTCACTTTAGGGACCTGTT+TGG + chr2.2:66331060-66331079 MS.gene43604:intron 40.0%
!! AGATGAGCATCGGAAAACAA+TGG + chr2.2:66333099-66333118 MS.gene43604:intron 40.0%
!! CCTTTTCACTTTTGGTCTTC+TGG + chr2.2:66330835-66330854 MS.gene43604:intron 40.0%
!! CTACTGTTGAAGAGTTTTGG+AGG + chr2.2:66330800-66330819 MS.gene43604:CDS 40.0%
!! TTGCCTGTGTGTTTTTATGC+CGG + chr2.2:66333178-66333197 MS.gene43604:intron 40.0%
!!! ATCGTCGTTGATGGATTTTG+CGG - chr2.2:66330569-66330588 None:intergenic 40.0%
ACACCGGCATAAAAACACAC+AGG - chr2.2:66333184-66333203 None:intergenic 45.0%
CTCACCCTCTTGAGAATTCT+TGG + chr2.2:66330698-66330717 MS.gene43604:CDS 45.0%
GACCCTATTTGTGCTAATGG+TGG + chr2.2:66331842-66331861 MS.gene43604:CDS 45.0%
GGGGAAAATCTGATACTAGC+TGG + chr2.2:66331888-66331907 MS.gene43604:CDS 45.0%
TGCTGTTAATGAAGCTGCTC+AGG + chr2.2:66332129-66332148 MS.gene43604:CDS 45.0%
TGGAGCAGTTGTGAATGTCA+GGG + chr2.2:66332072-66332091 MS.gene43604:CDS 45.0%
TGTGAATGTCAGGGATAAGG+CGG + chr2.2:66332081-66332100 MS.gene43604:CDS 45.0%
!! GAGCATCGGAAAACAATGGA+AGG + chr2.2:66333103-66333122 MS.gene43604:CDS 45.0%
GTGGAGCAGTTGTGAATGTC+AGG + chr2.2:66332071-66332090 MS.gene43604:CDS 50.0%
! AAGAGGGTGAGATTGGTGAC+CGG - chr2.2:66330689-66330708 None:intergenic 50.0%
!! GAACAGCCAACTTGCTAGGA+TGG - chr2.2:66331775-66331794 None:intergenic 50.0%
ATTTCCAAACCCCTCACCGC+CGG + chr2.2:66330667-66330686 MS.gene43604:CDS 55.0%
GGTCGAATCGAACTACCCCA+AGG - chr2.2:66330766-66330785 None:intergenic 55.0%
GGTGAGGGGTTTGGAAATGG+CGG - chr2.2:66330665-66330684 None:intergenic 55.0%
! GCTCGAACAGCCAACTTGCT+AGG - chr2.2:66331779-66331798 None:intergenic 55.0%
GAGGGGTTTGGAAATGGCGG+AGG - chr2.2:66330662-66330681 None:intergenic 60.0%
GGCGGTGAGGGGTTTGGAAA+TGG - chr2.2:66330668-66330687 None:intergenic 60.0%
! AGATTGGTGACCGGCGGTGA+GGG - chr2.2:66330680-66330699 None:intergenic 60.0%
! AGGGTGAGATTGGTGACCGG+CGG - chr2.2:66330686-66330705 None:intergenic 60.0%
! GAGATTGGTGACCGGCGGTG+AGG - chr2.2:66330681-66330700 None:intergenic 65.0%
! GATTGGTGACCGGCGGTGAG+GGG - chr2.2:66330679-66330698 None:intergenic 65.0%
GTGACCGGCGGTGAGGGGTT+TGG - chr2.2:66330674-66330693 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 66330550 66333321 66330550 ID=MS.gene43604
chr2.2 mRNA 66330550 66333321 66330550 ID=MS.gene43604.t1;Parent=MS.gene43604
chr2.2 exon 66330550 66330821 66330550 ID=MS.gene43604.t1.exon1;Parent=MS.gene43604.t1
chr2.2 CDS 66330550 66330821 66330550 ID=cds.MS.gene43604.t1;Parent=MS.gene43604.t1
chr2.2 exon 66331754 66331919 66331754 ID=MS.gene43604.t1.exon2;Parent=MS.gene43604.t1
chr2.2 CDS 66331754 66331919 66331754 ID=cds.MS.gene43604.t1;Parent=MS.gene43604.t1
chr2.2 exon 66332025 66332150 66332025 ID=MS.gene43604.t1.exon3;Parent=MS.gene43604.t1
chr2.2 CDS 66332025 66332150 66332025 ID=cds.MS.gene43604.t1;Parent=MS.gene43604.t1
chr2.2 exon 66333101 66333166 66333101 ID=MS.gene43604.t1.exon4;Parent=MS.gene43604.t1
chr2.2 CDS 66333101 66333166 66333101 ID=cds.MS.gene43604.t1;Parent=MS.gene43604.t1
chr2.2 exon 66333271 66333321 66333271 ID=MS.gene43604.t1.exon5;Parent=MS.gene43604.t1
chr2.2 CDS 66333271 66333321 66333271 ID=cds.MS.gene43604.t1;Parent=MS.gene43604.t1
Gene Sequence

>MS.gene43604

ATGGTTGTAGAAGAAACCGCAAAATCCATCAACGACGATCAAATCACAAACCCTAAAATCGAAGACATCAACAACGATCTCGAAGAAGGAGAGATCGACGACGATGATTCCTCCGCCATTTCCAAACCCCTCACCGCCGGTCACCAATCTCACCCTCTTGAGAATTCTTGGACTTTCTGGTTTGATAACCCTCAAGCTAAATCCAAAAGTAAACCTTGGGGTAGTTCGATTCGACCCGTCTACACTATTTCTACTGTTGAAGAGTTTTGGAGGTAAAGATTCTATCCTTTTCACTTTTGGTCTTCTGGCTCTTGAAAAAATTGTTCATTTGTTGTTTCTGGGATGAAAAGATTCATTTTTTATTTCTTGGGTGTTTTTAATTTTGGGTCAAAAGATTCAGTTTTTGTGAATTGGGTATTTTTTACATTAGGGTTTGTGATTCATTTTTAGTTAATTGGGTATTTCTTGTTTGTAAAATGTTGAAGAATTTTTTAGGTAAAGCTTCAATCTTTTCACTTTAGGGACCTGTTTGGATAAAGAGCTTATTTGCAGCTTATATTACAAGCGTTTATCAATATGCTTATCATGATATCCGCTTTTGTATAAGATTGCATAAGCTGTTTTTTAGCAAAAAAAATAATAACGCTGAATTGTTTTTTTATATGCTATAAGTTATTTCTATAAGCTATATTTGAGGACTTATGAAAATAAGGTGGAAAAAGCTTATGAAAATTGTCATAAGCTGTTTTCAAAAATTCTCCTAAACAGTCTCTCAAAAGATTCAATTTTTATGAATTGGGTATTTTTCACATTAGGGTTTGTGATTCATTTTTAGTTAATTGGGTATTTCTTGTTGTACAATGTTGAAGAATTTTTTAGGTAAAGCTTCGATCTTTTCACTTTAGGGACCTGTTTGGATAAAGAGCTTATTTGCAGCATATATTACAAGCGTTTATTATGTGCTTATCATGATAAACGCTAATGTATAAGCTTGCATAAAAATATCGTTCATTTATTGTTTCTTGGTTATTTTTGTATCTTGGGTATTTCTTGTTTGTAAAATGTTGCAGAAATTTTGAGGTAAAAATTCAATCTTTTCAACTTTAGGTCTTTTGGGTCATCAAAAAAATTGTTCATTTATTGATTCTTGGATATTTTTGTTTCTTAGTTATTCGTGTTACTGAAATTTTTATGGTTTTTTCAGCATTTACAATAATATTCACCATCCTAGCAAGTTGGCTGTTCGAGCTGATTTATATTGTTTCAAGCATAAGATTGAACCAAAATGGGAAGACCCTATTTGTGCTAATGGTGGAAATTGGACTGTGCAATTTTCTAGGGGAAAATCTGATACTAGCTGGTTGTATACGGTAGACTATTGAATTTGTTTTATGAGGGTATTATTTTGATTATTCTTTGTGATGAATTAAAGTACTAATTGGTCATGTATGTGGTTTTGTTCTTGGTAACTTTAGTTGTTGGCAATGATTGGAGAGCAATTTGATCATGGGGTTGAAATTTGTGGAGCAGTTGTGAATGTCAGGGATAAGGCGGAAAGAATTTCTATTTGGACTAAGAATGCTGTTAATGAAGCTGCTCAGGTAATTTCTTGTTCTTACTTTTGATTCAATAGGAACTAGATGGTTATGATGTTGAAGAGTAATATTATGATTTTCAAACTAAAGATGTGCATTTGTTATGTGACTGAGACCCCAAAGAAAGAATTAGATGGTTATTATAATGGCATGTATTCAATCACAGTTCATGAACAATTTAAATGGACATTTTTCACATTTTTGTTGCTTCAATGCCTGTGTTTCTTGTTTTCTCCCAATACCTTATTGTTTACAATTCAATCTAGATGATTGCTATAATGTTACAGATTATTGATTTTTCTAACCAACGATGAGCAGTTGTTATGTGAATGGCTCATTCATATCATTGCTGTTAAATATGAATGTAAAAAGCATGTATTCACAGTTCATCGGCTATTTAAATGGAAAGTTTTCACGTTTTTTGGTTATTCAATGCCTGTGTTTCTTGTTTTTCATCAATACCTTATTGGTTAGAGTGAACCACTAATATAGTCTCAGAAGTTTATAGACTTTGGTCAATTTGGCATCTGTAATTTACAGAATTTCTTAATGACCTCTGATATTGAAAATCCTCAATCAGTTAGACCTTTTTGGTCTATCAATTTAGTCTGAAATCATCATAATGTGATTGAGAATTTATATTTCAGGGATCACTCTCTCTTGTGAATTTTATTGATTTTAAGACAAATTGCCTAACTCAGCAATTTTAAAACTTAATTTATGATTCACTATTATTTTTTGGTAGAAGAGAATTTAAACTCTTGTAATTTGTCAGATGGAGAATGAATGTGTGATTATTATACATATGCTGCGACAATTCCATATAATTTACTCTTATGGCTTGTGAGACCAGAAATTATGTATCTGTTTATGCTTGTGCTGGTGTCTGTTTGAATCTACTGATTATACTTTTGATAATGGGGAACATATAAACTTAATTTTTTGTTATATTTCAGATGAGCATCGGAAAACAATGGAAGGAGTTTCTTGATTATAATGAAACCATTGGCTTTATATTTCATGTAAGTGGAACTTGCCTGTGTGTTTTTATGCCGGTGTGTTTTTATATTTGTCACTTCATCTTAAATCACCCTAACTTCTGTTTTGAATTGTGCTATTTCTGTAGGATGATGCAAAGAAGCTCGACAGAGCTGCTAAAAACAAATATGTTGTATGA
Protein sequence

>MS.gene43604.t1

MVVEETAKSINDDQITNPKIEDINNDLEEGEIDDDDSSAISKPLTAGHQSHPLENSWTFWFDNPQAKSKSKPWGSSIRPVYTISTVEEFWSIYNNIHHPSKLAVRADLYCFKHKIEPKWEDPICANGGNWTVQFSRGKSDTSWLYTLLAMIGEQFDHGVEICGAVVNVRDKAERISIWTKNAVNEAAQMSIGKQWKEFLDYNETIGFIFHDDAKKLDRAAKNKYVV