AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43614

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43614.t1 MTR_2g018260 92.982 228 13 2 1 228 1 225 4.35e-157 434
MS.gene43614.t1 MTR_1g045130 53.073 179 79 2 51 228 31 205 2.15e-64 199
MS.gene43614.t1 MTR_6g029230 36.478 159 99 1 41 199 40 196 8.09e-35 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43614.t1 AT4G18040 62.705 244 66 3 1 228 1 235 1.48e-105 304
MS.gene43614.t1 AT1G29550 54.321 243 93 4 1 228 1 240 8.94e-89 262
MS.gene43614.t1 AT1G29590 54.321 243 93 4 1 228 1 240 2.05e-88 261
MS.gene43614.t1 AT5G35620 50.000 184 84 5 38 215 12 193 4.05e-58 182
MS.gene43614.t1 AT5G35620 50.000 184 84 5 38 215 12 193 4.05e-58 182
MS.gene43614.t1 AT5G35620 48.148 162 76 5 31 186 5 164 2.42e-47 154
MS.gene43614.t1 AT5G18110 36.478 159 99 1 41 199 35 191 1.59e-30 112

Find 52 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAATTCTTGGACTTTC+TGG 0.032991 2.2:+66391043 MS.gene43614:CDS
TGATCGTCGTTGATGGATTT+TGG 0.121670 2.2:-66390904 None:intergenic
CAAATAGGGTCTTCCCATTT+TGG 0.162249 2.2:-66392166 None:intergenic
CTCACCCTCTTGAGAATTCT+TGG 0.186379 2.2:+66391034 MS.gene43614:CDS
AGCATAAGATTGAACCAAAA+TGG 0.214116 2.2:+66392152 MS.gene43614:CDS
AGGCGGAAAGAATTTCTATT+TGG 0.235213 2.2:+66392434 MS.gene43614:CDS
TTTCTACTGTTGAAGAGTTT+TGG 0.268111 2.2:+66391133 MS.gene43614:CDS
ACTACCCCAATCTTGACTTT+TGG 0.276521 2.2:-66391088 None:intergenic
AAGGAGTTTCTTGATTATAA+TGG 0.309476 2.2:+66393463 MS.gene43614:CDS
ATAGAAATTCTTTCCGCCTT+TGG 0.323105 2.2:-66392430 None:intergenic
GTGACCGGCGGTGAGGGGTT+TGG 0.329982 2.2:-66391007 None:intergenic
CTTCCACCATTAGCACAAAT+AGG 0.334237 2.2:-66392181 None:intergenic
CTTTAGTTGTTGGCAATGAT+TGG 0.357757 2.2:+66392355 MS.gene43614:intron
AGATTGGTGACCGGCGGTGA+GGG 0.372856 2.2:-66391013 None:intergenic
TAAATCCAAAAGTCAAGATT+GGG 0.376321 2.2:+66391083 MS.gene43614:CDS
TGTGATTTGATCGTCGTTGA+TGG 0.385098 2.2:-66390911 None:intergenic
GGCGGTGAGGGGTTTGGAAA+TGG 0.408337 2.2:-66391001 None:intergenic
TGATACTAGCTGGTTGTATA+CGG 0.414513 2.2:+66392234 MS.gene43614:CDS
CTTGATTATAATGGAACCAT+TGG 0.423062 2.2:+66393472 MS.gene43614:CDS
CTAAATCCAAAAGTCAAGAT+TGG 0.429593 2.2:+66391082 MS.gene43614:CDS
AATTCTCAAGAGGGTGAGAT+TGG 0.439429 2.2:-66391029 None:intergenic
TTCCACCATTAGCACAAATA+GGG 0.451905 2.2:-66392180 None:intergenic
TGCTGCTAATGAAGCTGCTC+AGG 0.454593 2.2:+66392465 MS.gene43614:CDS
GCATAAGATTGAACCAAAAT+GGG 0.459310 2.2:+66392153 MS.gene43614:CDS
CAATCTTATGCTTGAAACAA+TGG 0.479043 2.2:-66392141 None:intergenic
GATCATGGAGTTGAAATTTG+TGG 0.481177 2.2:+66392388 MS.gene43614:CDS
ATTGGAGAGCAATTTGATCA+TGG 0.490081 2.2:+66392373 MS.gene43614:CDS
GCATAAACAGCCAACTTGCT+AGG 0.501919 2.2:-66392112 None:intergenic
GTGGAGCAGTTGTGAATGTC+AGG 0.501928 2.2:+66392407 MS.gene43614:CDS
GAAGACCCTATTTGTGCTAA+TGG 0.505377 2.2:+66392175 MS.gene43614:CDS
GGTGAGGGGTTTGGAAATGG+CGG 0.516008 2.2:-66390998 None:intergenic
GTTATATTTCAGATGAGCAT+CGG 0.524149 2.2:+66393430 MS.gene43614:intron
GGGGAAAATCTGATACTAGC+TGG 0.537034 2.2:+66392224 MS.gene43614:CDS
AAACAGCCAACTTGCTAGGA+TGG 0.537147 2.2:-66392108 None:intergenic
ATCAACAACGATCTCGAAGA+AGG 0.542418 2.2:+66390952 MS.gene43614:CDS
TTTGTGCTAATGGTGGAAGA+TGG 0.558543 2.2:+66392185 MS.gene43614:CDS
GATTGGTGACCGGCGGTGAG+GGG 0.563209 2.2:-66391012 None:intergenic
GAGATTGGTGACCGGCGGTG+AGG 0.563789 2.2:-66391014 None:intergenic
CATTCACCATCCTAGCAAGT+TGG 0.564197 2.2:+66392102 MS.gene43614:CDS
ATTTCCAAACCCCTCACCGC+CGG 0.575370 2.2:+66391003 MS.gene43614:CDS
GAGGGGTTTGGAAATGGCGG+AGG 0.595654 2.2:-66390995 None:intergenic
GAGCATCGGAAAACAATGGA+AGG 0.597596 2.2:+66393444 MS.gene43614:CDS
TACATGAAATATAAAGCCAA+TGG 0.599495 2.2:-66393488 None:intergenic
GACCCTATTTGTGCTAATGG+TGG 0.609050 2.2:+66392178 MS.gene43614:CDS
AAGAGGGTGAGATTGGTGAC+CGG 0.612893 2.2:-66391022 None:intergenic
AGATGAGCATCGGAAAACAA+TGG 0.613189 2.2:+66393440 MS.gene43614:intron
AGTTGTGAATGTCAGGCCAA+AGG 0.629792 2.2:+66392414 MS.gene43614:CDS
AAAGTCCAAGAATTCTCAAG+AGG 0.638231 2.2:-66391039 None:intergenic
AAGTCCAAGAATTCTCAAGA+GGG 0.640086 2.2:-66391038 None:intergenic
TGTGAATGTCAGGCCAAAGG+CGG 0.646359 2.2:+66392417 MS.gene43614:CDS
AAATCCAAAAGTCAAGATTG+GGG 0.670525 2.2:+66391084 MS.gene43614:CDS
AGGGTGAGATTGGTGACCGG+CGG 0.679762 2.2:-66391019 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGTTGAAGAATTTTTT+CGG + chr2.2:66391359-66391378 MS.gene43614:intron 15.0%
!!! AAGATTCATTTTTTATTTCT+TGG + chr2.2:66391233-66391252 MS.gene43614:intron 15.0%
!!! AGATTCATTTTTTATTTCTT+GGG + chr2.2:66391234-66391253 MS.gene43614:intron 15.0%
!!! TATGATTCACTATTATTTTT+TGG + chr2.2:66393200-66393219 MS.gene43614:intron 15.0%
!!! TGATTCATTTTTAGTTAATT+GGG + chr2.2:66391322-66391341 MS.gene43614:intron 15.0%
!! AATTGTAAACAATAAGGTAT+TGG - chr2.2:66392715-66392734 None:intergenic 20.0%
!! AGTTTGAATTGTAAACAATA+AGG - chr2.2:66392721-66392740 None:intergenic 20.0%
!! ATTTCTATAAGCTATATTTG+AGG + chr2.2:66391559-66391578 MS.gene43614:intron 20.0%
!! GAATTAGATGGTTATTATAA+TGG + chr2.2:66392603-66392622 MS.gene43614:intron 20.0%
!! GTTTGAGAATTTATATTTCA+GGG + chr2.2:66393105-66393124 MS.gene43614:intron 20.0%
!! TGTTTGAGAATTTATATTTC+AGG + chr2.2:66393104-66393123 MS.gene43614:intron 20.0%
!! TTCCATATAATTTACTCTTA+TGG + chr2.2:66393298-66393317 MS.gene43614:intron 20.0%
!! TTGTCAGCTATTTATTTAAA+TGG + chr2.2:66392863-66392882 MS.gene43614:intron 20.0%
!!! AAGATTCAGTTTTTATGAAT+TGG + chr2.2:66391661-66391680 MS.gene43614:intron 20.0%
!!! AATTCAATCTTTTCAACTTT+AGG + chr2.2:66391971-66391990 MS.gene43614:intron 20.0%
!!! ACAATGTTGAAGAATTTTTT+AGG + chr2.2:66391743-66391762 MS.gene43614:intron 20.0%
!!! ACTGATTATACTTTTGATAA+TGG + chr2.2:66393384-66393403 MS.gene43614:intron 20.0%
!!! AGATTCAGTTTTTATGAATT+GGG + chr2.2:66391662-66391681 MS.gene43614:intron 20.0%
!!! ATTGTAAACAATAAGGTATT+GGG - chr2.2:66392714-66392733 None:intergenic 20.0%
!!! ATTGTTCATTTATTGATTCT+TGG + chr2.2:66392014-66392033 MS.gene43614:intron 20.0%
!!! CTGATTATACTTTTGATAAT+GGG + chr2.2:66393385-66393404 MS.gene43614:intron 20.0%
!!! CTTTTGAATTTGTTTTATGA+GGG + chr2.2:66392261-66392280 MS.gene43614:intron 20.0%
!!! GTGATTCATTTTTAGTTAAT+TGG + chr2.2:66391321-66391340 MS.gene43614:intron 20.0%
!!! TATTAAAAGCGTTTATCAAT+AGG + chr2.2:66391442-66391461 MS.gene43614:intron 20.0%
!!! TGATTATACTTTTGATAATG+GGG + chr2.2:66393386-66393405 MS.gene43614:intron 20.0%
! AACCATCTAATTCTTTCTTT+GGG - chr2.2:66392596-66392615 None:intergenic 25.0%
! AAGGAGTTTCTTGATTATAA+TGG + chr2.2:66393463-66393482 MS.gene43614:CDS 25.0%
! ACAATTCAAAACAGAAGTTA+GGG - chr2.2:66393579-66393598 None:intergenic 25.0%
! ACTAAATTGATAGACCAAAA+AGG - chr2.2:66393067-66393086 None:intergenic 25.0%
! AGCCATAAGAGTAAATTATA+TGG - chr2.2:66393303-66393322 None:intergenic 25.0%
! CAGTTCATGAACAATTTAAA+TGG + chr2.2:66392640-66392659 MS.gene43614:intron 25.0%
! TAAATCCAAAAGTCAAGATT+GGG + chr2.2:66391083-66391102 MS.gene43614:CDS 25.0%
! TAACCATCTAATTCTTTCTT+TGG - chr2.2:66392597-66392616 None:intergenic 25.0%
! TACATGAAATATAAAGCCAA+TGG - chr2.2:66393491-66393510 None:intergenic 25.0%
! TATGCAATCTTATACAAAAG+CGG - chr2.2:66391481-66391500 None:intergenic 25.0%
! TTTGAGGACTTATGAAAATA+AGG + chr2.2:66391575-66391594 MS.gene43614:intron 25.0%
!! AAAATGTTGCAGAAATTTTG+AGG + chr2.2:66391944-66391963 MS.gene43614:intron 25.0%
!! AAGATTCAGTTTTTGTGAAT+TGG + chr2.2:66391277-66391296 MS.gene43614:intron 25.0%
!! AATTGGGTATTTTTCACATT+AGG + chr2.2:66391678-66391697 MS.gene43614:intron 25.0%
!! AGATTCAGTTTTTGTGAATT+GGG + chr2.2:66391278-66391297 MS.gene43614:intron 25.0%
!! ATCGTTCATTTATTGTTTCT+TGG + chr2.2:66391889-66391908 MS.gene43614:intron 25.0%
!! GTTTTTCATCAATACCTTAT+TGG + chr2.2:66392928-66392947 MS.gene43614:intron 25.0%
!! TCATGTTACTGAAATTTTCA+TGG + chr2.2:66392058-66392077 MS.gene43614:intron 25.0%
!! TTTTCAACTTTAGGTCTTTT+GGG + chr2.2:66391980-66391999 MS.gene43614:intron 25.0%
!!! AATTGGGTATTTTTTACGTT+AGG + chr2.2:66391294-66391313 MS.gene43614:intron 25.0%
!!! ATTGGGTATTTTTTACGTTA+GGG + chr2.2:66391295-66391314 MS.gene43614:intron 25.0%
!!! ATTGTTCATTTGTTGTTTCT+TGG + chr2.2:66391205-66391224 MS.gene43614:intron 25.0%
!!! CTTGGTTATTTTTGTATCTT+GGG + chr2.2:66391907-66391926 MS.gene43614:intron 25.0%
!!! GATTCTATCTTTTTCACTTT+TGG + chr2.2:66391163-66391182 MS.gene43614:intron 25.0%
!!! GCTTTTGAATTTGTTTTATG+AGG + chr2.2:66392260-66392279 MS.gene43614:intron 25.0%
!!! TCTTGGGTGTTTTTAATTTT+GGG + chr2.2:66391250-66391269 MS.gene43614:intron 25.0%
!!! TCTTGGTTATTTTTGTATCT+TGG + chr2.2:66391906-66391925 MS.gene43614:intron 25.0%
!!! TCTTGTACTTTTGATTCAAT+AGG + chr2.2:66392493-66392512 MS.gene43614:intron 25.0%
!!! TGATGAATGAAAGTACTAAT+TGG + chr2.2:66392305-66392324 MS.gene43614:intron 25.0%
!!! TTCTTGGGTGTTTTTAATTT+TGG + chr2.2:66391249-66391268 MS.gene43614:intron 25.0%
!!! TTTTAAAATTGCTGAGAGTT+AGG - chr2.2:66393173-66393192 None:intergenic 25.0%
AAATCCAAAAGTCAAGATTG+GGG + chr2.2:66391084-66391103 MS.gene43614:CDS 30.0%
AACTTCAGAGACTATATTAG+TGG - chr2.2:66392964-66392983 None:intergenic 30.0%
AATAAGCTCTTTATCCAAAC+AGG - chr2.2:66391413-66391432 None:intergenic 30.0%
ACCATCTAATTCTTTCTTTG+GGG - chr2.2:66392595-66392614 None:intergenic 30.0%
AGCATAAGATTGAACCAAAA+TGG + chr2.2:66392152-66392171 MS.gene43614:CDS 30.0%
AGTTTAGACTTTGGTCAATT+TGG + chr2.2:66392980-66392999 MS.gene43614:intron 30.0%
CAAGCATAAACAGATACATA+TGG - chr2.2:66393343-66393362 None:intergenic 30.0%
CAATCTTATGCTTGAAACAA+TGG - chr2.2:66392144-66392163 None:intergenic 30.0%
CACAATTCAAAACAGAAGTT+AGG - chr2.2:66393580-66393599 None:intergenic 30.0%
CAGATACATATGGTAATTTC+TGG - chr2.2:66393333-66393352 None:intergenic 30.0%
CTAAATCCAAAAGTCAAGAT+TGG + chr2.2:66391082-66391101 MS.gene43614:CDS 30.0%
GCATAAGATTGAACCAAAAT+GGG + chr2.2:66392153-66392172 MS.gene43614:CDS 30.0%
GTTATATTTCAGATGAGCAT+CGG + chr2.2:66393430-66393449 MS.gene43614:intron 30.0%
TGATGAAAAACAAGAAACAC+AGG - chr2.2:66392919-66392938 None:intergenic 30.0%
TTGATTCAATAGGAACTAGA+TGG + chr2.2:66392503-66392522 MS.gene43614:intron 30.0%
! AACAGTAGAAAAAGTGTAGA+CGG - chr2.2:66391124-66391143 None:intergenic 30.0%
! AACTCTTGTAATTTGTCAGA+TGG + chr2.2:66393237-66393256 MS.gene43614:intron 30.0%
! AGCTTCAATCTTTTCACTTT+AGG + chr2.2:66391385-66391404 MS.gene43614:intron 30.0%
! CTTCAATCTTTTCACTTTAG+GGG + chr2.2:66391387-66391406 MS.gene43614:intron 30.0%
! CTTGATTATAATGGAACCAT+TGG + chr2.2:66393472-66393491 MS.gene43614:CDS 30.0%
! CTTTTCAACTTTAGGTCTTT+TGG + chr2.2:66391979-66391998 MS.gene43614:intron 30.0%
! GCTTCAATCTTTTCACTTTA+GGG + chr2.2:66391386-66391405 MS.gene43614:intron 30.0%
! GGCTTTATATTTCATGTAAG+TGG + chr2.2:66393493-66393512 MS.gene43614:intron 30.0%
! TGGAAAGTTTTCACGTTTTT+TGG + chr2.2:66392883-66392902 MS.gene43614:intron 30.0%
! TGTTGATGTCTTCGATTTTA+GGG - chr2.2:66390939-66390958 None:intergenic 30.0%
! TTCTTGGTAACTTTAGTTGT+TGG + chr2.2:66392345-66392364 MS.gene43614:intron 30.0%
! TTGAGGATTTTCAATATCAG+AGG - chr2.2:66393035-66393054 None:intergenic 30.0%
! TTGTTGATGTCTTCGATTTT+AGG - chr2.2:66390940-66390959 None:intergenic 30.0%
! TTTCTACTGTTGAAGAGTTT+TGG + chr2.2:66391133-66391152 MS.gene43614:CDS 30.0%
!! AATCTTTTGAGAGACTGTTT+AGG - chr2.2:66391647-66391666 None:intergenic 30.0%
!! TTGAATTGTGCTATTTCTGT+AGG + chr2.2:66393590-66393609 MS.gene43614:intron 30.0%
AAAGTCCAAGAATTCTCAAG+AGG - chr2.2:66391042-66391061 None:intergenic 35.0%
AAGTCCAAGAATTCTCAAGA+GGG - chr2.2:66391041-66391060 None:intergenic 35.0%
ACCCCAAAGAAAGAATTAGA+TGG + chr2.2:66392591-66392610 MS.gene43614:intron 35.0%
AGGCGGAAAGAATTTCTATT+TGG + chr2.2:66392434-66392453 MS.gene43614:CDS 35.0%
ATAGAAATTCTTTCCGCCTT+TGG - chr2.2:66392433-66392452 None:intergenic 35.0%
ATGAGCAGTTGTTATGTGAA+TGG + chr2.2:66392787-66392806 MS.gene43614:intron 35.0%
ATTGGAGAGCAATTTGATCA+TGG + chr2.2:66392373-66392392 MS.gene43614:CDS 35.0%
CAAAAAGGTCTAACTGATTG+AGG - chr2.2:66393052-66393071 None:intergenic 35.0%
CTTTAGTTGTTGGCAATGAT+TGG + chr2.2:66392355-66392374 MS.gene43614:intron 35.0%
GAGGACTTATGAAAATAAGG+TGG + chr2.2:66391578-66391597 MS.gene43614:intron 35.0%
GATCATGGAGTTGAAATTTG+TGG + chr2.2:66392388-66392407 MS.gene43614:CDS 35.0%
GTATCTGTTTATGCTTGTGT+TGG + chr2.2:66393346-66393365 MS.gene43614:intron 35.0%
TGATACTAGCTGGTTGTATA+CGG + chr2.2:66392234-66392253 MS.gene43614:CDS 35.0%
TTCCACCATTAGCACAAATA+GGG - chr2.2:66392183-66392202 None:intergenic 35.0%
TTGAGAATTCTTGGACTTTC+TGG + chr2.2:66391043-66391062 MS.gene43614:CDS 35.0%
! ACAGTAGAAAAAGTGTAGAC+GGG - chr2.2:66391123-66391142 None:intergenic 35.0%
! AGCTTCGATCTTTTCACTTT+AGG + chr2.2:66391769-66391788 MS.gene43614:intron 35.0%
! CTCAATCAGTTAGACCTTTT+TGG + chr2.2:66393050-66393069 MS.gene43614:intron 35.0%
! GCTTCGATCTTTTCACTTTA+GGG + chr2.2:66391770-66391789 MS.gene43614:intron 35.0%
!! AGTCTCTGAAGTTTAGACTT+TGG + chr2.2:66392971-66392990 MS.gene43614:intron 35.0%
!! ATGGACTGTGCAATTTTCTA+GGG + chr2.2:66392204-66392223 MS.gene43614:CDS 35.0%
!! CATGTGTGTGTTTTTGTTCT+TGG + chr2.2:66392329-66392348 MS.gene43614:intron 35.0%
!! CTTTTTCACTTTTGGTCTTC+TGG + chr2.2:66391171-66391190 MS.gene43614:intron 35.0%
!!! GACTTTTGGATTTAGCTTGA+GGG - chr2.2:66391077-66391096 None:intergenic 35.0%
!!! TGACTTTTGGATTTAGCTTG+AGG - chr2.2:66391078-66391097 None:intergenic 35.0%
AATTCTCAAGAGGGTGAGAT+TGG - chr2.2:66391032-66391051 None:intergenic 40.0%
ACACCAGCATAAAAACACAC+AGG - chr2.2:66393525-66393544 None:intergenic 40.0%
ACATAACAACTGCTCATCGT+TGG - chr2.2:66392784-66392803 None:intergenic 40.0%
ATCAACAACGATCTCGAAGA+AGG + chr2.2:66390952-66390971 MS.gene43614:CDS 40.0%
CTTCCACCATTAGCACAAAT+AGG - chr2.2:66392184-66392203 None:intergenic 40.0%
GAAGACCCTATTTGTGCTAA+TGG + chr2.2:66392175-66392194 MS.gene43614:CDS 40.0%
GTGGTTCGCTTTAACCAATA+AGG - chr2.2:66392945-66392964 None:intergenic 40.0%
TGGGAGAAAACAAGAAACAC+AGG - chr2.2:66392695-66392714 None:intergenic 40.0%
TGTGATTTGATCGTCGTTGA+TGG - chr2.2:66390914-66390933 None:intergenic 40.0%
! ACTACCCCAATCTTGACTTT+TGG - chr2.2:66391091-66391110 None:intergenic 40.0%
! CAAATAGGGTCTTCCCATTT+TGG - chr2.2:66392169-66392188 None:intergenic 40.0%
! TGGACTGTGCAATTTTCTAG+GGG + chr2.2:66392205-66392224 MS.gene43614:CDS 40.0%
! TTTCACTTTAGGGACCTGTT+TGG + chr2.2:66391780-66391799 MS.gene43614:intron 40.0%
! TTTGTGCTAATGGTGGAAGA+TGG + chr2.2:66392185-66392204 MS.gene43614:CDS 40.0%
!! AGATGAGCATCGGAAAACAA+TGG + chr2.2:66393440-66393459 MS.gene43614:intron 40.0%
!! CTACTGTTGAAGAGTTTTGG+AGG + chr2.2:66391136-66391155 MS.gene43614:CDS 40.0%
!! TTGCCTGTGTGTTTTTATGC+TGG + chr2.2:66393519-66393538 MS.gene43614:intron 40.0%
!!! ATCGTCGTTGATGGATTTTG+GGG - chr2.2:66390905-66390924 None:intergenic 40.0%
!!! GATCGTCGTTGATGGATTTT+GGG - chr2.2:66390906-66390925 None:intergenic 40.0%
!!! GATGGACTGTGCAATTTTCT+AGG + chr2.2:66392203-66392222 MS.gene43614:CDS 40.0%
!!! TGATCGTCGTTGATGGATTT+TGG - chr2.2:66390907-66390926 None:intergenic 40.0%
ACTAGCTGGTTGTATACGGT+AGG + chr2.2:66392238-66392257 MS.gene43614:intron 45.0%
AGTTGTGAATGTCAGGCCAA+AGG + chr2.2:66392414-66392433 MS.gene43614:CDS 45.0%
CATTCACCATCCTAGCAAGT+TGG + chr2.2:66392102-66392121 MS.gene43614:CDS 45.0%
CTCACCCTCTTGAGAATTCT+TGG + chr2.2:66391034-66391053 MS.gene43614:CDS 45.0%
GACCCTATTTGTGCTAATGG+TGG + chr2.2:66392178-66392197 MS.gene43614:CDS 45.0%
GGGGAAAATCTGATACTAGC+TGG + chr2.2:66392224-66392243 MS.gene43614:CDS 45.0%
! GCATAAACAGCCAACTTGCT+AGG - chr2.2:66392115-66392134 None:intergenic 45.0%
! TTTCACTTTAGGGGCCTGTT+TGG + chr2.2:66391396-66391415 MS.gene43614:intron 45.0%
!! AAACAGCCAACTTGCTAGGA+TGG - chr2.2:66392111-66392130 None:intergenic 45.0%
!! GAGCATCGGAAAACAATGGA+AGG + chr2.2:66393444-66393463 MS.gene43614:CDS 45.0%
GTGGAGCAGTTGTGAATGTC+AGG + chr2.2:66392407-66392426 MS.gene43614:CDS 50.0%
TGCTGCTAATGAAGCTGCTC+AGG + chr2.2:66392465-66392484 MS.gene43614:CDS 50.0%
TGTGAATGTCAGGCCAAAGG+CGG + chr2.2:66392417-66392436 MS.gene43614:CDS 50.0%
! AAGAGGGTGAGATTGGTGAC+CGG - chr2.2:66391025-66391044 None:intergenic 50.0%
ATTTCCAAACCCCTCACCGC+CGG + chr2.2:66391003-66391022 MS.gene43614:CDS 55.0%
GGTGAGGGGTTTGGAAATGG+CGG - chr2.2:66391001-66391020 None:intergenic 55.0%
GAGGGGTTTGGAAATGGCGG+AGG - chr2.2:66390998-66391017 None:intergenic 60.0%
GGCGGTGAGGGGTTTGGAAA+TGG - chr2.2:66391004-66391023 None:intergenic 60.0%
! AGATTGGTGACCGGCGGTGA+GGG - chr2.2:66391016-66391035 None:intergenic 60.0%
! AGGGTGAGATTGGTGACCGG+CGG - chr2.2:66391022-66391041 None:intergenic 60.0%
! GAGATTGGTGACCGGCGGTG+AGG - chr2.2:66391017-66391036 None:intergenic 65.0%
! GATTGGTGACCGGCGGTGAG+GGG - chr2.2:66391015-66391034 None:intergenic 65.0%
GTGACCGGCGGTGAGGGGTT+TGG - chr2.2:66391010-66391029 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 66390886 66393668 66390886 ID=MS.gene43614
chr2.2 mRNA 66390886 66393668 66390886 ID=MS.gene43614.t1;Parent=MS.gene43614
chr2.2 exon 66390886 66391157 66390886 ID=MS.gene43614.t1.exon1;Parent=MS.gene43614.t1
chr2.2 CDS 66390886 66391157 66390886 ID=cds.MS.gene43614.t1;Parent=MS.gene43614.t1
chr2.2 exon 66392090 66392255 66392090 ID=MS.gene43614.t1.exon2;Parent=MS.gene43614.t1
chr2.2 CDS 66392090 66392255 66392090 ID=cds.MS.gene43614.t1;Parent=MS.gene43614.t1
chr2.2 exon 66392361 66392486 66392361 ID=MS.gene43614.t1.exon3;Parent=MS.gene43614.t1
chr2.2 CDS 66392361 66392486 66392361 ID=cds.MS.gene43614.t1;Parent=MS.gene43614.t1
chr2.2 exon 66393442 66393507 66393442 ID=MS.gene43614.t1.exon4;Parent=MS.gene43614.t1
chr2.2 CDS 66393442 66393507 66393442 ID=cds.MS.gene43614.t1;Parent=MS.gene43614.t1
chr2.2 exon 66393612 66393668 66393612 ID=MS.gene43614.t1.exon5;Parent=MS.gene43614.t1
chr2.2 CDS 66393612 66393668 66393612 ID=cds.MS.gene43614.t1;Parent=MS.gene43614.t1
Gene Sequence

>MS.gene43614

ATGGTTGTAGAAGAAACCCCAAAATCCATCAACGACGATCAAATCACAAACCCTAAAATCGAAGACATCAACAACGATCTCGAAGAAGGAGAGATCGACGACGATGATTCCTCCGCCATTTCCAAACCCCTCACCGCCGGTCACCAATCTCACCCTCTTGAGAATTCTTGGACTTTCTGGTTTGATAACCCTCAAGCTAAATCCAAAAGTCAAGATTGGGGTAGTTCGATTCGACCCGTCTACACTTTTTCTACTGTTGAAGAGTTTTGGAGGTAAAGATTCTATCTTTTTCACTTTTGGTCTTCTGGCTCTTGAAAAAATTGTTCATTTGTTGTTTCTTGGATGAAAAGATTCATTTTTTATTTCTTGGGTGTTTTTAATTTTGGGTCAAAAGATTCAGTTTTTGTGAATTGGGTATTTTTTACGTTAGGGTTTGTGATTCATTTTTAGTTAATTGGGTATTTCTTGTTTGTAAAATGTTGAAGAATTTTTTCGGTAAAGCTTCAATCTTTTCACTTTAGGGGCCTGTTTGGATAAAGAGCTTATTTGCAGCTTATATTAAAAGCGTTTATCAATAGGCTTATCATGATATCCGCTTTTGTATAAGATTGCATAAGCTGTTTTTTAGCAAAAAAATAATAACGTTGAATTGTTTTTTTATATGCTATAAGTTATTTCTATAAGCTATATTTGAGGACTTATGAAAATAAGGTGGAAAAAGCTTATGAAAATTGTCATAAGCTGTTTTCATAAATTCTCCTAAACAGTCTCTCAAAAGATTCAGTTTTTATGAATTGGGTATTTTTCACATTAGGATTTGTGATTCATTTTTAGTTAATTGGGTATTTCTTGTTTGTACAATGTTGAAGAATTTTTTAGGTAAAGCTTCGATCTTTTCACTTTAGGGACCTGTTTGGATAAAGAGCTTATTTGCAGCTTATATCACAAGCGTTTATTATGTGCTTATCATGATAAGCGCTAATGTATAAGCTTGCATAAAAATATCGTTCATTTATTGTTTCTTGGTTATTTTTGTATCTTGGGTATTTCTTGTTTGTAAAATGTTGCAGAAATTTTGAGGTAAAAATTCAATCTTTTCAACTTTAGGTCTTTTGGGTCATCAAAAATATTGTTCATTTATTGATTCTTGGATATTTTTGTTTCTTAGTTATTCATGTTACTGAAATTTTCATGGTTTTTTCAGCATTTACAATAACATTCACCATCCTAGCAAGTTGGCTGTTTATGCTGATTTCCATTGTTTCAAGCATAAGATTGAACCAAAATGGGAAGACCCTATTTGTGCTAATGGTGGAAGATGGACTGTGCAATTTTCTAGGGGAAAATCTGATACTAGCTGGTTGTATACGGTAGGCTTTTGAATTTGTTTTATGAGGGTATTATTTTGATTATTCTTTGTGATGAATGAAAGTACTAATTGGTCATGTGTGTGTTTTTGTTCTTGGTAACTTTAGTTGTTGGCAATGATTGGAGAGCAATTTGATCATGGAGTTGAAATTTGTGGAGCAGTTGTGAATGTCAGGCCAAAGGCGGAAAGAATTTCTATTTGGACTAAGAATGCTGCTAATGAAGCTGCTCAGGTAATTTCTTGTACTTTTGATTCAATAGGAACTAGATGGTTATGATGTTGAAGAGTAATATTATGATTTTCAAACTAAAGATGTGCATTTGTTATGTGACTGAGACCCCAAAGAAAGAATTAGATGGTTATTATAATGGCATGTATTCAATCACAGTTCATGAACAATTTAAATGGACATTTTTCACATTTTTGTTGCTTCAATGCCTGTGTTTCTTGTTTTCTCCCAATACCTTATTGTTTACAATTCAAACTAGATGATTGCTATAGTGTTACAGATTATTGATTTTTCTAACCAACGATGAGCAGTTGTTATGTGAATGGCTCATTCATATCATTGCTGTTAAATATGAATGTAAAAAGCATGTATTCACAGTTTGTCAGCTATTTATTTAAATGGAAAGTTTTCACGTTTTTTGGTTATTCAATGCCTGTGTTTCTTGTTTTTCATCAATACCTTATTGGTTAAAGCGAACCACTAATATAGTCTCTGAAGTTTAGACTTTGGTCAATTTGGCATCTGTAATTTACAAAATTTCTTAATGACCTCTGATATTGAAAATCCTCAATCAGTTAGACCTTTTTGGTCTATCAATTTAGTCTGAAATCATCATAATGTGTTTGAGAATTTATATTTCAGGGATCACTCTCTCTTGTGAATTTTATTGATTTTAAGACAAATTGCCTAACTCTCAGCAATTTTAAAACTTAATTTATGATTCACTATTATTTTTTGGTAGAAGAGAATTTAAACTCTTGTAATTTGTCAGATGGAGAATGAATGTGTGATTATTATAAATATGCTGCAACAATTCCATATAATTTACTCTTATGGCTTGTGAGACCAGAAATTACCATATGTATCTGTTTATGCTTGTGTTGGTGTCTGTTTGAATCTACTGATTATACTTTTGATAATGGGGAACATATAAACTTAATTTTTTGTTATATTTCAGATGAGCATCGGAAAACAATGGAAGGAGTTTCTTGATTATAATGGAACCATTGGCTTTATATTTCATGTAAGTGGAACTTGCCTGTGTGTTTTTATGCTGGTGTGTTTTTATATTTGTCACTTCATCTTAAATCACCCTAACTTCTGTTTTGAATTGTGCTATTTCTGTAGGATGATGCAAAGAAGCTCGACAGAGCTGCAGCTGCTAAAAACAAATATGTTGTATGA
Protein sequence

>MS.gene43614.t1

MVVEETPKSINDDQITNPKIEDINNDLEEGEIDDDDSSAISKPLTAGHQSHPLENSWTFWFDNPQAKSKSQDWGSSIRPVYTFSTVEEFWSIYNNIHHPSKLAVYADFHCFKHKIEPKWEDPICANGGRWTVQFSRGKSDTSWLYTLLAMIGEQFDHGVEICGAVVNVRPKAERISIWTKNAANEAAQMSIGKQWKEFLDYNGTIGFIFHDDAKKLDRAAAAKNKYVV